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20. Microsatellite records for volume 11, issue 1

Author

Chen, Da-Qing, Dong, Zaijie, Fang, Min, Fu, Jianjun, Jia, Xiang-Yang, Jiang, Bingjie, Li, Jingmiao, Li, Qingqing, Liao, Suhui, Liu, Ping, Liu, Yulin, Shu, Guocheng, Shu, Xiaoxiao, Sun, Xuefang, Huiwu-Tian, Wang, Lanmei, Wei, Lan, Xie, Feng, and Zhu, Wenbin

Published
2019
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31. Screening and Identification of Interacting Proteins of Mitfa in Red Tilapia.

Author

Jiang, Bingjie, Wang, Lanmei, Fu, Jianjun, Zhu, Wenbin, Luo, Mingkun, and Dong, Zaijie

Subjects
PROTEOMICS, TILAPIA, GENE expression, RECOMBINANT proteins, FISH skin, MELANOGENESIS
Abstract

Skin color variation and differentiation are bottlenecks in the aquaculture industry of red tilapia. The mitfa gene primarily regulates melanocyte survival and proliferation in fish skin pigmentation. In this study, a recombinant expression vector for Mitfa expression was successfully constructed. After induction and purification, nearly 600 μg of Mitfa protein was purified from the fermentation supernatant. His pull-down assay, followed by LC-MS/MS analysis, revealed 10 proteins that interact with the Mitfa protein. Five of these proteins were annotated in the melanogenesis and tyrosine metabolism pathways, including Krt, Tpm, Myh, Tyr and Dct. The purified Mitfa recombinant protein was injected into red tilapia, and its activity and function were examined. The expression levels of the mitfa, tyr, tyrp1, and dct genes increased greatly in the dorsal skin after injection (p < 0.05); meanwhile, the melanin content and tyrosinase activity increased significantly (p < 0.05). These findings provide a theoretical basis for comprehending the function and regulatory mechanism of the red tilapia mitfa gene. [ABSTRACT FROM AUTHOR]

Published
2023
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34. Transcriptome Analysis of Skin Color Variation During and after Overwintering of Malaysian Red Tilapia

Author

mingkun Luo, Zhu Wenbin, Dong Zaijie, Jianjun Fu, Bingjie Jiang, and Lanmei Wang

Subjects
Transcriptome, food.ingredient, food, Variation (linguistics), Skin color, Zoology, Tilapia, Biology, Overwintering
Abstract

The commercial value of red tilapia is hampered by variations in skin color during overwintering. In this study, three types skin of red tilapia, including the skin remained pink color during and after overwintering (WP), the skin changed from pink color to black color during overwintering and remained black color after overwintering (PB), and the skin changed from pink color to black color during overwintering but recovered to pink color when the temperature rose after overwintering (BP), were used to analyze their molecular mechanisms of color variation. The transcriptome results revealed that the PB, WP, and BP libraries had 42, 43, and 43 million clean reads, respectively. The top 10 abundance mRNAs and specific mRNAs (specificity measure SPM > 0.9) were screened. After comparing intergroup gene expression levels, there were 2528, 1924 and 1939 differentially expressed genes (DEGs) between BP and PB, BP and WP, and PB and WP, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of color-related mRNAs showed that a number of DEGs, including tyrp1, tyr, pmel, mitf, mc1r, asip, tat, hpdb and foxd3, might play a potential role in pigmentation. Additionally, the co-expression patterns of genes were detected within the pigment-related pathways by PPI network from PB_WP group. Furthermore, DEGs from the apoptosis and autophagy pathways, such as baxα, beclin1, and atg7, might be involved in the fading of red tilapia melanocytes. The findings will aid in understanding the molecular mechanism underlying skin color variation in red tilapia during and after overwintering, as well as lay a foundation for future research aimed at improving red tilapia skin color characteristics.

Published
2021

35. Cover image

Author

Luo, Mingkun, primary, Lu, Guoqing, additional, Yin, Haoran, additional, Wang, Lanmei, additional, Atuganile, Malambugi, additional, and Dong, Zaijie, additional

Published
2021
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43. Cover Image

Author

Yin, Haoran, primary, Luo, Mingkun, additional, Luo, Wentao, additional, Wang, Lanmei, additional, Zhu, Wenbin, additional, Fu, Jianjun, additional, and Dong, Zaijie, additional

Published
2020
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49. Comparative analysis of the gut microbiota in bighead carp under different culture patterns.

Author

Luo, Mingkun, An, Rui, Fu, Jianjun, Wan, Shunpeng, Zhu, Wenbin, Wang, Lanmei, and Dong, Zaijie

Subjects
BIGHEAD carp, GUT microbiome, CARP, NILE tilapia, FISH farming, GASTROINTESTINAL contents, MULTIDIMENSIONAL scaling
Abstract

Aims: To investigate the phylogenetic composition and functional potential of bighead carp (Hypophthalmichthys nobilis) gut microbiome in two rearing patterns (bighead carp polycultured with Oreochromis niloticus in pond A and bighead carp polycultured with Cyprinus carpio in pond B, respectively), as well as the changes of plankton in the cultured water at four different time points. Methods and Results: The intestinal contents were sequenced using Illumina HiSeq of bacterial 16S rRNA. Cyanophyta and Chlorophyta were the prevalent phytoplankton in the water, whereas Rotifers and Protozoa were the predominant zooplankton. In all, 779,563 quality‐filtered sequences and 8870 amplicon sequence variants were obtained from 24 samples that numbered T1A1 to T4A3 and T1B1 to T4B3, resulting in 35 phyla, with Proteobacteria, Firmicutes, Fusobacteria and Cyanobacteria dominating. According to alpha diversity and beta diversity measurements, the bacterial communities were diverse, Chao1 richness and Pielou's evenness were significantly lower in the T2B and T4B groups. The gut bacterial communities of T1A, T1B, T2A and T2B groups differed from those of other samples, which formed distinctly clusters with principal coordinate analysis and non‐metric multidimensional scaling analysis. PICRUSt2 predictive function analysis revealed that different culture patterns influenced the gut microbiota metabolic capacity. Conclusions: Intestinal bacteria belonging to the phyla Proteobacteria, Firmicutes, Cyanobacteria and Fusobacteria are better suited to inhabit in various environments and perform specific functions. Furthermore, contact with the external environment and nutrient intake also stimulate the variety of intestinal microbiotas in polycultured bighead carp. Significance and Impact of the Study: This is the first comprehensive, high‐throughput investigation of gut microbiota diversity in bighead carp during various seasons in two polycultured patterns and provide preliminary information on gut microbiome composition and changes, laying a crucial foundation for future research on fish culture patterns in various environments. [ABSTRACT FROM AUTHOR]

Published
2022
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