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Additional file 2: Fig. S2. The ultrastructure of HSPCs changed. The photomicrographs of TEM showed that nuclear membrane, perinuclear cisternae, chromatin edge, inclusion body in cytoplasm of HSPC changed. Old mouse HSPC showed similar but less morphologic changes than model HSPC.

Additional file 1: Fig. S1. The percentage of SA-β-gal stain-positive cells. The percentage of SA-β-gal stain-positive cells increased gradually at day2, 4, 6, 8.

Additional file 3: Fig. S3. Proteomics KEGG pathway analysis of differentially expressed proteins. (a,b), Pyruvate kinase were significantly increased and pyruvate dehydrogenase E1were reduced in the model cells. (c,d), Dkc1, GAR1, Nop10, Nog1 and ribosomal proteins in the ribosome-sized subunits also decreased significantly in the model cells. (e), U2AF1 (U2AF) 1 were significantly decreased in the model cells. (f), Lysosomal related proteins such as Cathepsin, TPP1, lysosomal membrane protein LIMP, lysosomal-associated membrane protein (LAMP), and ATPeV were significantly increased in the model cells.