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1,939 results on '"Dong, Xian"'

5. Correlation of distribution characteristics and dynamic changes of gut microbiota with the efficacy of immunotherapy in EGFR-mutated non-small cell lung cancer

Author

Luo, Wei-Chi, Mei, Shi-Qi, Huang, Zi-Jian, Chen, Zhi-Hong, Zhang, Yi-Chen, Yang, Ming-Yi, Liu, Jia-Qi, Xu, Jing-Yan, Yang, Xiao-Rong, Zhong, Ri-Wei, Tang, Li-Bo, Yin, Lin-Xi, Deng, Yu, Peng, Ying-Long, Lu, Chang, Chen, Bao-Long, Ke, Dong-Xian, Tu, Hai-Yan, Yang, Jin-Ji, Xu, Chong-Rui, Wu, Yi-Long, and Zhou, Qing

Published
2024
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24. Water masses influence the variation of microbial communities in the Yangtze River Estuary and its adjacent waters

Author

Wen-Dong Xian, Jinhui Chen, Zheng Zheng, Junjie Ding, Yinli Xi, Yiying Zhang, Wu Qu, Chunyu Tang, Changlin Li, Xuezhu Liu, Wei Li, and Jianxin Wang

Subjects
Yangtze river estuary, water masses, bacterial diversity, network topology, community assembly, Microbiology, QR1-502
Abstract

The Yangtze River estuary (YRE) are strongly influenced by the Kuroshio and terrigenous input from rivers, leading to the formation of distinct water masses, however, there remains a limited understanding of the full extent of this influence. Here the variation of water masses and bacterial communities of 58 seawater samples from the YRE and its adjacent waters were investigated. Our findings suggested that there were 5 water masses in the studied area: Black stream (BS), coastal water in the East China Sea (CW), nearshore mixed water (NM), mixed water in the middle and deep layers of the East China Sea (MM), and deep water blocks in the middle of the East China Sea (DM). The CW mass harbors the highest alpha diversity across all layers, whereas the NM mass exhibits higher diversity in the surface layer but lower in the middle layers. Proteobacteria was the most abundant taxa in all water masses, apart from that, in the surface layer masses, Cyanobacterium, Bacteroidota, and Actinobacteriota were the highest proportion in CW, while Bacteroidota and Actinobacteriota were the highest proportion in NM and BS; in the middle layer, Bacteroidota and Actinobacteriota were dominant phylum in CW and BS masses, but Cyanobacterium was main phylum in NM mass; in the bottom layer, Bacteroidota and Actinobacteriota were the dominant phylum in CW, while Marininimicrobia was the dominated phylum in DM and MM masses. Network analysis suggests water masses have obvious influence on community topological characteristics, moreover, community assembly across masses also differ greatly. Taken together, these results emphasized the significant impact of water masses on the bacterial composition, topological characteristics and assembly process, which may provide a theoretical foundation for predicting alterations in microbial communities within estuarine ecosystems under the influence of water masses.

Published
2024
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31. Lysosomal Potassium Channels

Author

Huang, Peng, Xu, Mengnan, Wu, Yi, Rizvi Syeda, Alia Kazim, Dong, Xian-Ping, Michel, Martin C., Editor-in-Chief, Barrett, James E., Editorial Board Member, Centurión, David, Editorial Board Member, Flockerzi, Veit, Editorial Board Member, Geppetti, Pierangelo, Editorial Board Member, Hofmann, Franz B., Editorial Board Member, Meier, Kathryn Elaine, Editorial Board Member, Page, Clive P., Editorial Board Member, Wang, KeWei, Editorial Board Member, Wahl-Schott, Christian, editor, and Biel, Martin, editor

Published
2023
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37. Characterization of a thermophilic and glucose-tolerant GH1 β-glucosidase from hot springs and its prospective application in corn stover degradation

Author

Yu-Ying Huang, Zhi-Hua Lv, Hong-Zhao Zheng, Qian Zhu, Meng-Ting Liu, Peng Sang, Fei Wang, Dan Zhu, Wen-Dong Xian, and Yi-Rui Yin

Subjects
hot spring, β-glucosidase, thermophilic, thermostability, glucose tolerance, Microbiology, QR1-502
Abstract

Introductionβ-Glucosidase serves as the pivotal rate-limiting enzyme in the cellulose degradation process, facilitating the hydrolysis of cellobiose and cellooligosaccharides into glucose. However, the widespread application of numerous β-glucosidases is hindered by their limited thermostability and low glucose tolerance, particularly in elevated-temperature and high-glucose environments.MethodsThis study presents an analysis of a β-glucosidase gene belonging to the GH1 family, denoted lqbg8, which was isolated from the metagenomic repository of Hehua hot spring located in Tengchong, China. Subsequently, the gene was cloned and heterologously expressed in Escherichia coli BL21(DE3). Post expression, the recombinant β-glucosidase (LQBG8) underwent purification through a Ni affinity chromatography column, thereby enabling the in-depth exploration of its enzymatic properties.ResultsLQBG8 had an optimal temperature of 70°C and an optimum pH of 5.6. LQBG8 retained 100 and 70% of its maximum activity after 2-h incubation periods at 65°C and 70°C, respectively. Moreover, even following exposure to pH ranges of 3.0–10.0 for 24 h, LQBG8 retained approximately 80% of its initial activity. Notably, the enzymatic prowess of LQBG8 remained substantial at glucose concentrations of up to 3 M, with a retention of over 60% relative activity. The kinetic parameters of LQBG8 were characterized using cellobiose as substrate, with Km and Vmax values of 28 ± 1.9 mg/mL and 55 ± 3.2 μmol/min/mg, respectively. Furthermore, the introduction of LQBG8 (at a concentration of 0.03 mg/mL) into a conventional cellulase reaction system led to an impressive 43.7% augmentation in glucose yield from corn stover over a 24-h period. Molecular dynamics simulations offered valuable insights into LQBG8’s thermophilic nature, attributing its robust stability to reduced fluctuations, conformational changes, and heightened structural rigidity in comparison to mesophilic β-glucosidases.DiscussionIn summation, its thermophilic, thermostable, and glucose-tolerant attributes, render LQBG8 ripe for potential applications across diverse domains encompassing food, feed, and the production of lignocellulosic ethanol.

Published
2023
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45. Thermophilic Dehalococcoidia with unusual traits shed light on an unexpected past

Author

Marike Palmer, Jonathan K Covington, En-Min Zhou, Scott C Thomas, Neeli Habib, Cale O Seymour, Dengxun Lai, Juliet Johnston, Ameena Hashimi, Jian-Yu Jiao, Alise R Muok, Lan Liu, Wen-Dong Xian, Xiao-Yang Zhi, Meng-Meng Li, Leslie P Silva, Benjamin P Bowen, Katherine Louie, Ariane Briegel, Jennifer Pett-Ridge, Peter K Weber, Elitza I Tocheva, Tanja Woyke, Trent R Northen, Xavier Mayali, Wen-Jun Li, and Brian P Hedlund

Published
2023
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