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Your search keyword '"Dong, Tenghui"' showing total 23 results
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23 results on '"Dong, Tenghui"'

8. Research on the speed‐dependence of heat convection in high‐speed EV motors and its application in the model‐based rotor temperature monitoring technology

Author

Dong, Tenghui, Nakamura, Taketsune, Dong, Tenghui, and Nakamura, Taketsune

Abstract

Using the embedded onboard digital thermal model is an economical and effective way to monitor the rotor temperatures of the electric vehicle (EV) propulsion Permanent Magnet Synchronous Motor (PMSM) in real-time. The onboard thermal model is generally in an analytical form with constant thermal resistances. However, in practical situations, the thermal convection intensities on the surfaces of the air gap and the end-cavity vary significantly with the rotational speed, producing a non-negligible influence on the estimation accuracy of the onboard thermal model. Aiming at eliminating this error and improving the accuracy, this paper explores the variation of the airflow's aerodynamics characteristics and the heat convection with the rotational speed through a syncretic study of theoretical, experimental, and numerical methods. It is found that the changing trend of the airflow presents a multi-stage characteristic in the speed range of 0–12, 000 rpm. The speed-dependent convective thermal resistances are formulaically parameterised and then used to replace the related constant thermal resistances in the thermal model. The standard vehicle driving cycle test result shows that this optimisation brings a nearly 11% reduction of the overall estimation error.

Published
2022

21. Methylation of RASSF10 Promotes Cell Proliferation and Serves as a Docetaxel Resistant Marker in Human Breast Cancer

Author

Dong, Tenghui, Zhang, Meiying, Dong, Yan, Herman, James G., van Engeland, Manon, Zhong, Guanglin, Guo, Mingzhou, Pathologie, RS: GROW - Oncology, and RS: GROW - R2 - Basic and Translational Cancer Biology

Abstract

Breast cancer is a leading cause of death among females worldwide. However, the mechanisms of breast cancer remain largely unclear. RASSF10 is frequently methylated in certain types of human cancers. To understand the mechanism of RASSF10 in breast cancer, samples of breast cancer cell lines, primary cancer, breast tissue adjacent to cancer, and normal breast tissue were investigated in this study. Methylation specific PCR (MSP), flow cytometry, western blot, and siRNA knockdown assay were used. Complete methylation was found in MCF7, MDA-MB-435, and MDA-MB-468 cells, partial methylation was found in ZR75-1 cells, and unmethylation was revealed in SKBR3 cells. Loss of RASSF10 expression was found in MCF7, MDA-MB-435, and MDA-MB-468 cells, and high expression of RASSF10 was found in ZR75-1 and SKBR3 cells. The expression of RASSF10 was induced by 5-aza-2'-deoxycytidine (5-Aza) in MCF7, MDA-MB435, and MDA-MB-468 cells. RASSF10 methylation was found in 77.8% (49/63) of primary breast cancer and 17.8% (8/45) of adjacent tissue samples. No methylation was found in normal breast tissue samples. Restoration of RASSF10 expression inhibited cell proliferation and induced G2/M phase arrest in MCF7 and MDA-MB-468 cells. RASSF10 sensitized these cells to docetaxel. RASSF10 induced apoptosis and activated P53 signaling in breast cancer cells. In conclusion, RASSF10 is frequently methylated in human breast cancer and infrequently in adjacent tissue samples. RASSF10 methylation may serve as an early detective marker. The expression of RASSF10 is regulated by promoter region methylation. Since restoration of RASSF10 expression sensitized breast cancer cells to docetaxel, RASSF10 methylation is a potential docetaxel resistant marker. RASSF10 suppresses breast cancer growth by activating P53 signaling.

Published
2015

23. Methylation of RASSF10 promotes cell proliferation and serves as a docetaxel resistant marker in human breast cancer.

Author

Dong T, Zhang M, Dong Y, Herman JG, van Engeland M, Zhong G, and Guo M

Subjects
Biomarkers, Tumor genetics, Breast Neoplasms, DNA, Neoplasm genetics, DNA, Neoplasm metabolism, Docetaxel, Female, Gene Expression Regulation, Neoplastic drug effects, Humans, MCF-7 Cells, Signal Transduction drug effects, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Tumor Suppressor Proteins genetics, Biomarkers, Tumor biosynthesis, Cell Proliferation drug effects, DNA Methylation, Drug Resistance, Neoplasm drug effects, Taxoids pharmacology, Tumor Suppressor Proteins biosynthesis
Abstract

Breast cancer is a leading cause of death among females worldwide. However, the mechanisms of breast cancer remain largely unclear. RASSF10 is frequently methylated in certain types of human cancers. To understand the mechanism of RASSF10 in breast cancer, samples of breast cancer cell lines, primary cancer, breast tissue adjacent to cancer, and normal breast tissue were investigated in this study. Methylation specific PCR (MSP), flow cytometry, western blot, and siRNA knockdown assay were used. Complete methylation was found in MCF7, MDA-MB-435, and MDA-MB-468 cells, partial methylation was found in ZR75-1 cells, and unmethylation was revealed in SKBR3 cells. Loss of RASSF10 expression was found in MCF7, MDA-MB-435, and MDA-MB-468 cells, and high expression of RASSF10 was found in ZR75-1 and SKBR3 cells. The expression of RASSF10 was induced by 5-aza-2'-deoxycytidine (5-Aza) in MCF7, MDA-MB-435, and MDA-MB-468 cells. RASSF10 methylation was found in 77.8% (49/63) of primary breast cancer and 17.8% (8/45) of adjacent tissue samples. No methylation was found in normal breast tissue samples. Restoration of RASSF10 expression inhibited cell proliferation and induced G2/M phase arrest in MCF7 and MDA-MB-468 cells. RASSF10 sensitized these cells to docetaxel. RASSF10 induced apoptosis and activated P53 signaling in breast cancer cells. In conclusion, RASSF10 is frequently methylated in human breast cancer and infrequently in adjacent tissue samples. RASSF10 methylation may serve as an early detective marker. The expression of RASSF10 is regulated by promoter region methylation. Since restoration of RASSF10 expression sensitized breast cancer cells to docetaxel, RASSF10 methylation is a potential docetaxel resistant marker. RASSF10 suppresses breast cancer growth by activating P53 signaling.

Published
2015

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