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23 results on '"Dong, Haiqiang"'

6. CRISPR/Cas9‐mediated mutations of FANTASTIC FOUR gene family for creating early flowering mutants in tomato.

Author

Shang, Lele, Tao, Jinbao, Song, Jianwen, Wang, Yaru, Zhang, Xingyu, Ge, Pingfei, Li, Fangman, Dong, Haiqiang, Gai, Wenxian, Grierson, Donald, Ye, Zhibiao, and Zhang, Yuyang

Subjects
GENE families, TOMATO breeding, FLOWERING time, CRISPRS, AGRICULTURE, TOMATOES
Abstract

Summary: Flowering time is of great agricultural importance and the timing and extent of flowering usually determines yield and availability of flowers, fruits and seeds. Identification of genes determining flowering has important practical applications for tomato breeding. Here we demonstrate the roles of the FANTASTIC FOUR (FAF) gene family in regulating tomato flowering time. In this plant‐specific gene family, SlFAF1/2a shows a constitutive expression pattern during the transition of the shoot apical meristem (SAM) from vegetative to reproductive growth and significantly influences flowering time. Overexpressing SlFAF1/2a causes earlier flowering compared with the transformations of other genes in the FAF family. SlFAF1/2c also positively regulates tomato flowering, although to a lesser extent. The other members of the SlFAF gene family, SlFAF1/2b, SlFAF3/4a and SlFAF3/4b, are negative regulators of tomato flowering and faf1/2b, faf3/4a and faf3/4b single mutants all display early flowering. We generated a series of early flowering mutants using the CRISPR/Cas9 editing system, and the faf1/2b faf3/4a faf3/4b triple mutant flowering earliest compared with other mutants. More importantly, these mutants show no adverse effect on yield. Our results have uncovered the role of the FAF gene family in regulating tomato flowering time and generated early flowering germplasms for molecular breeding. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Multiple-model GWAS identifies optimal allelic combinations of quantitative trait loci for malic acid in tomato

Author

Gai, Wenxian, primary, Yang, Fan, additional, Yuan, Liangdan, additional, ul Haq, Saeed, additional, Wang, Yaru, additional, Wang, Ying, additional, Shang, Lele, additional, Li, Fangman, additional, Ge, Pingfei, additional, Dong, Haiqiang, additional, Tao, Jinbao, additional, Wang, Fei, additional, Zhang, Xingyu, additional, and Zhang, Yuyang, additional

Published
2023
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9. A 21-bp InDel in the promoter of STP1 selected during tomato improvement accounts for soluble solid content in fruits

Author

Wang, Ying, primary, Shi, Chunmei, additional, Ge, Pingfei, additional, Li, Fangman, additional, Zhu, Lihui, additional, Wang, Yaru, additional, Tao, Jinbao, additional, Zhang, Xingyu, additional, Dong, Haiqiang, additional, Gai, Wenxian, additional, Wang, Fei, additional, Ye, Zhibiao, additional, Grierson, Donald, additional, Xu, Wei, additional, and Zhang, Yuyang, additional

Published
2023
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12. Exogenous γ-Aminobutyric Acid (GABA) Treatment Suppresses Ethylene Biosynthesis in Hardy Kiwifruit (Actinidia aruguta) During Postharvest Storage

Author

Dong, Haiqiang, Lan, Qingqing, Zhou, Yuwen, Zhou, Yufei, Bao, Zeyang, Wen, Qingde, Xiang, Chongjian, Zheng, Caixia, and Li, Xu

Subjects
horticulture
Abstract

γ-Aminobutyric acid (GABA) plays an important role in plant development and postharvest properties of fruits. However, studies on regulation of kiwifruit ripening by GABA are scarce. Here, we evaluted physicochemical characters and the expression profile of genes related to ripening in hardy kiwifruit treated with exogenous GABA compared to control during postharvest storage period. The results showed that (1) exogenous GABA treatment not only improved the fruit quality but also inhibited respiration rate and ethylene production, as well as reduced the enzyme activities of ACC oxidase (ACO) and ACC synthase (ACS), via down-regulating the expression of AaACO1 and AaACO3, AaACS1 and AaACS2 during kiwifruit storage period; (2) on the one hand, overexpression of AaGAD1 or AaGAD4 gene which is involved in GABA biosynthesis inhibited ethylene production by reducing the enzyme activities of ACO and ACS, whereas silencing of AaGAD1 or AaGAD4 gene led to the reverse effect. On the other hand, overexpression of AaGAD1 or AaGAD4 decreased the expression levels of AaACO1 and AaACO3, AaACS1 and AaACS2, whereas silencing of AaGAD1 or AaGAD4 gene increased the expression levels of these four genes. Above all, our findings demonstrate that exogenous GABA treatment could improve the storage quality and extend the shelf life of kiwifruit, Additionally, the content of GABA was participated in the regulation of ethylene biosynthesis at the molecular level.

Published
2022

16. Effect of shading on ascorbic acid accumulation and biosynthetic gene expression during tomato fruit development and ripening

Author

Tamim, Safir Ahmad, primary, Li, Fangman, additional, Wang, Ying, additional, Shang, Lele, additional, Zhang, Xingyu, additional, Tao, Jinbao, additional, Wang, Yaru, additional, Gai, Wenxian, additional, Dong, Haiqiang, additional, Ahiakpa, John Kojo, additional, Mumtaz, Muhammad Ali, additional, and Zhang, Yuyang, additional

Published
2022
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21. A 21-bp InDel in the promoter of STP1selected during tomato improvement accounts for soluble solid content in fruits

Author

Wang, Ying, Shi, Chunmei, Ge, Pingfei, Li, Fangman, Zhu, Lihui, Wang, Yaru, Tao, Jinbao, Zhang, Xingyu, Dong, Haiqiang, Gai, Wenxian, Wang, Fei, Ye, Zhibiao, Grierson, Donald, Xu, Wei, and Zhang, Yuyang

Abstract

Domestication and improvement are important processes that generate the variation in genome and phonotypes underlying crop improvement. Unfortunately, during selection for certain attributes, other valuable traits may be inadvertently discarded. One example is the decline in fruit soluble solids content (SSC) during tomato breeding. Several genetic loci for SSC have been identified, but few reports on the underlying mechanisms are available. In this study we performed a genome-wide association study (GWAS) for SSC of the red-ripe fruits in a population consisting of 481 tomato accessions with large natural variations and found a new quantitative trait locus, STP1, encoding a sugar transporter protein. The causal variation of STP1, a 21-bp InDel located in the promoter region 1124 bp upstream of the start codon, alters its expression. STP1Insertionaccessions with an 21-bp insertion have higher SSC than STP1Deletionaccessions with the 21-bp deletion. Knockout of STP1in TS-23 with high SSC using CRISPR/Cas9 greatly decreased SSC in fruits. In vivoand in vitroassays demonstrated that ZAT10-LIKE, a zinc finger protein transcription factor (ZFP TF), can specifically bind to the promoter of STP1Insertionto enhance STP1expression, but not to the promoter of STP1Deletion, leading to lower fruit SSC in modern tomatoes. Diversity analysis revealed that STP1was selected during tomato improvement. Taking these results together, we identified a naturally occurring causal variation underlying SSC in tomato, and a new role for ZFP TFs in regulating sugar transporters. The findings enrich our understanding of tomato evolution and domestication, and provide a genetic basis for genome design for improving fruit taste.

Published
2023
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22. The Artificial Promoter rMdAG2I Confers Flower-specific Activity in Malus.

Author

Dong, Haiqiang, Liu, Lanxin, Fan, Xingqiang, Asghar, Sumeera, Li, Yi, Wang, Yi, Xu, Xuefeng, Wu, Ting, Zhang, Xinzhong, Qiu, Changpeng, Han, Zhenhai, and Li, Wei

Subjects
*ORNAMENTAL plants, *NICOTIANA benthamiana, *APPLES, *COLLISIONS (Nuclear physics), *PROMOTERS (Genetics), *ARABIDOPSIS thaliana, *AGROBACTERIUM
Abstract

Genetic modifications of floral organs are important in the breeding of Malus species. Flower-specific promoters can be used to improve floral organs specifically, without affecting vegetative organs, and therefore developing such promoters is highly desirable. Here, we characterized two paralogs of the Arabidopsis thaliana gene AGAMOUS (AG) from Malus domestica (apple): MdAG1 and MdAG2. We then isolated the second-intron sequences for both genes, and created four artificial promoters by fusing each intron sequence to a minimal 35S promoter sequence in both the forward and reverse directions. When transferred into tobacco (Nicotiana benthamiana) by Agrobacterium tumefaciens-mediated stable transformation, one promoter, rMdAG2I, exhibited activity specifically in flowers, whereas the other three also showed detectable activity in vegetative organs. A test of the four promoters' activities in the ornamental species Malus micromalus by Agrobacterium-mediated transient transformation showed that, as in tobacco, only rMdAG2I exhibited a flower-specific expression pattern. Through particle bombardment transformation, we demonstrated that rMdAG2I also had flower-specific activity in the apple cultivar 'Golden Delicious'. The flower-specific promoter rMdAG2I, derived from M. domestica, thus has great potential for use in improving the floral characteristics of ornamental plants, especially the Malus species. [ABSTRACT FROM AUTHOR]

Published
2019
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