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In 2023, the European Commission released a legislative proposal for a Directive on Soil Monitoring and Resilience which aims to define a legal framework to achieve healthy soils across the European Union (EU) by 2050. A key component of the initial Directive is the mandate for Member States to establish basic geographic soil governance units, referred to as soil districts, and appoint a district-specific authority to oversee the implementation of soil health assessments. This paper proposes an operational definition of the districts following the conditions outlined in the proposal for the Directive and discusses various attention points for their implementation. Tentative districts were developed for seven EU countries, considering soil type, climate, topography, and land cover factors, starting from the smallest existing administrative unit (i.e. municipalities). Experts were asked to report on the applicability of the proposed districts within well-known pedo-ecological regions and discuss the relevance of the districts for establishing an EU-wide monitoring network and reporting on soil health and degradation. The outcomes highlight the need for detailed soil maps to account for specific soil types when stratifying countries into soil districts. The soilscape approach allows for a consistent method to defining soil districts across Member States. This enables contrasting soils within a district to be managed in a similar manner, with soil degradation/health thresholds applied to each district based on land cover. However, it is unclear whether soil districts as currently formulated in the Directive are in fact the right tool to support local soil management and monitoring of soil health. Districts can help ensure that all soil conditions are covered in a monitoring system, but they may not provide support for soil management or monitoring at a local scale due to short-scale soil variability and threats affecting soil management within the same soilscape. Beyond the use of districts for designing a European/national scale monitoring system, the districts can help create animations and other educational tools to promote soil literacy and connectivity of users to soils locally.

Various stakeholders, such as modelers, policy makers, farmers, and environmental regulators need reliable soil bulk density and coarse fragment content data. These two soil parameters are necessary to calculate soil carbon and nutrients stocks, to estimate water availability for plants, or to assess soil compaction. However, measuring these two parameters is labor intensive and time consuming. Therefore, many agricultural and environmental studies often miss these two soil parameters. Here, we provide four datasets, one with bulk density and coarse fragment contents of topsoil and subsoil, measured in two campaigns of the French Soil Quality Monitoring Network (RMQS for its acronym in French), a second one with the average values for bulk density and coarse fragments of the two campaigns at 0–30 cm and 30–50 cm. The third and the fourth ones are the raw data needed to calculate the two first datasets divided by campaign. In addition, the R script for calculating the depth-weighted values per soil layer is provided

A recent assessment states that 60–70% of soils in Europe are considered degraded. Protecting such valuable resource require knowledge on soil status through monitoring systems. In Europe, different types of monitoring networks currently exist in parallel. Many EU Member states (MS) developed their own national soil information monitoring system (N-SIMS), some being in place for decades. In parallel in 2009, the European Commission extended the periodic Land Use/Land Cover Area Frame Survey (LUCAS) led by EUROSTAT to sample and analyse the main properties of topsoil in EU in order to develop a homogeneous dataset for EU.Both sources of information are needed to support European policies on soil health evaluation. However, a question remains whether the assessment obtained by using soil properties from both monitoring programs (N-SIMS and LUCAS Soil) are comparable, and what could be the limitations of using either one dataset or the other.Conducted in the context of European Joint Programme (EJP) SOIL, this study shows the results of a comparison between N-SIMS and LUCAS Soil programs among 12 different EU member states including BE, DE, DK, EE, ES, FR, DE, HU, IT, NL, PL, SE and SK. The comparison was done on: (i) the sampling strategies including site densities, land cover and soil type distribution; (ii) the statistical distribution of three soil properties (organic carbon, pH and clay content); (iii) two potential indicators of soil quality (i.e. OC/Clay ratio and pH classes). The results underlined substantial differences in soil properties statistical distributions between N-SIMS and LUCAS Soil in many member states, particularly for woodland and grassland soils, affecting the evaluation of soil health using indicators. Such differences might be explained by both the monitoring strategy and sampling or analytical protocols exposing the potential effect of data source on European and national policies. The results demonstrate the need to work towards data harmonization and in the light of the Soil Monitoring Law, to carefully design the future of soil monitoring in Europe taking into account both LUCAS Soil and N-SIMS considering the significant impact of the monitoring strategies and protocols on soil health indicators.

We provide six short building blocks to devise adaptable soil definitions. These buiding blocks start from a simple and straightforward metaphor and progressively evolve when adopting geophysical, analytical, biogeochemical, cultural, and services-driven points of view. These progressive changes end up with a straightforward, yet most important message for a broad audience. We discuss these building blocks by comparing them to recently proposed soil definitions. Our aims are to initiate a debate on i) what should be, or should not be named soil, ii) what a soil definition should be, iii) the need for both a generic definition and a set of adaptable definitions, based on building blocks, commonly accepted by soil scientists, and iv) the need to adapt our language to diverse audiences. Finally, we propose a definition in one sentence.

Soil compaction is one of the most important and readily mitigated threats to soil health. Digital Soil Mapping (DSM) has emerged as an efficient method to provide broad-scale maps by combining soil information with environmental covariates. Until now, soil information input to DSM has been mainly composed of point-based quantitative measurements of soil properties and/or of soil type/horizon classes derived from laboratory analysis, point observations, or soil maps. In this study, we used field estimates of soil compaction to map soil behavior to compaction at a national scale. The results from a previous study enabled clustering of six different behaviors using the in situ field observations. Mapping potential responses to soil compaction is an effective land management tool for preventing future compaction. Random forest was used to make spatial predictions of soil behavior to compaction over cultivated soils of mainland France (about 210,000 km2). Modeling was performed at 90 m resolution. The map enabled us to spatially identify clusters of possible responses to compaction. Most clusters were consistent with known geographic distributions of some soil types and properties. This consistency was checked by comparing maps with both national and local-scale external sources of soil information. The best spatial predictors were available digital maps of soil properties (clay, silt, sand, organic carbon (SOC) content, and pH), some indicators of soil structural quality using SOC and clay content, and environmental covariates (T °C and relief-related covariates). Predicted maps were interpretable to support management recommendations to mitigate soil compactness at the soil–scape scale. Simple observational field data that are usually collected by soil surveyors, then stored and available in soil databases, provide valuable input data for digital mapping of soil behavior to compaction and assessment of inherent soil sensitivity to compaction.

The soil organic carbon (SOC) to clay-sized particles ratio (SOC/clay) has recently been selected as an indicator of the soil organic matter status in managed mineral soils within the framework of the European Soil Monitoring Law proposal. This indicator was initially developed to predict soil structural quality in a local study in Switzerland and was subsequently tested at national scales in England and Wales, and in Germany. In this study, we evaluated if the SOC/clay ratio was relevant to assess the structural quality of soils at the national scale in mainland France. We additionally evaluated its variant, SOC/(silt

Digital soil mapping (DSM) is commonly conducted using input soil attributes derived from laboratory analyses of geographically referenced samples. Field observations are often abundant and can offer a dense source of soil data that has the potential to enhance DSM predictions. However, they are not widely used due to to concerns about subjectivity and data quality. This study investigates the usefulness of hand-feel soil texture (HFST) data for DSM. We processed HFST data obtained from forest soils in France from two inventory campaigns: (i) HFST determination from systematic 1 km2 grid observations in France utilizing a specialized soil texture triangle, and (ii) HFST observations from soil survey samples, using a different texture triangle. Both sets of HFST data were used as input soil variables, with the same covariates, for predicting topsoil texture In a sizable, forested area through a DSM method. By employing independent sampling and laboratory soil analyses in selected areas, we uncovered measurement bias in one of the datasets. However, intriguingly, these biased observations identified subtle yet highly specific and unexpected patterns of sands in terraces due to alluvial deposits along small rivers. Thus, field soil observations, even if they are biased, should not be dismissed solely based on their overall predictive performance. It is essential to carefully examine predicted maps and covariates to determine whether patterns may have pedological and/or lithological origins and if they are pertinent for enhancing DSM predictions, enhancing soil process understanding, and meeting the requirements of end users. Numerous HFST are available worldwide, these datasets are usually disregarded for DSM. Here we contend that efforts should be put in recovering these data, and their potential for enhancing DSM and deepening our understanding of soil processes.

Soils are a finite resource that is under threat, mainly due to human pressure. Therefore, there is an urgent need to produce maps of soil properties, functions and behaviors that can support land management and various stakeholders’ decisions. Compaction is a major threat to soil functions, such as water infiltration and storage, and crops’ root growth. However, there is no general agreement on a universal and easy-to-implement indicator of soil susceptibility to compaction. The proposed indicators of soil compaction require numerous analytical determinations (mainly bulk density measurements) that are cost prohibitive to implement. In this study, we used data collected in numerous in situ topsoil observations during conventional soil survey and compared field observations to usual indicators of soil compactness. We unraveled the relationships between field estimates of soil compactness and measured soil properties. Most of the quantitative indicators proposed by the literature were rather consistent with the ordering of soil compactness classes observed in the field. The best relationship was obtained with an indicator using bulk density and clay (BDr2) to define three classes of rooting limitation. We distinguished six clusters of topsoil behaviors using hierarchical clustering. These clusters exhibited different soil behaviors to compaction that were related to soil properties, such as particle-size fractions, pH, CaCO3 and organic carbon content, cation exchange capacity, and some BDr2 threshold values. We demonstrate and discuss the usefulness of field observations to assess topsoil behavior to compaction. The main novelty of this study is the use of large numbers of qualitative field observations of soil profiles and clustering to identify contrasting behavior. To our knowledge, this approach has almost never been implemented. Overall, analysis of qualitative and quantitative information collected in numerous profiles offers a new way to discriminate some broad categories of soil behavior that could be used to support land management and stakeholders’ decisions.

Soil organic carbon (SOC) plays a crucial role in soil health and global carbon cycling, therefore accurate estimates of its spatial distribution are important for managing soil health and mitigating global climate change. Digital soil mapping shows its potential to provide accurate and high-resolution spatial distribution of SOC across scales. To convert SOC content to SOC density (SOCD), two inference trajectories exist for predicting SOCD in digital soil mapping: the direct approach (calculate-then-model) and indirect approach (model-then-calculate). However, there is a lack of comprehensive exploration regarding the differences in their performance in SOCD estimates, particularly in regions characterized by diverse pedoclimatic conditions. To bridge this knowledge gap, we evaluated the two approaches based on model performance of SOCD in France. Using 916 topsoils (0−20 ​cm) from the LUCAS Soil 2018 and 24 environmental covariates, random forest model and forward recursive feature selection were used to build the spatial predictive models of SOCD using direct and indirect approaches. The results show that, using random forest model and full covariates, both approaches show moderate performance (R2 ​= ​0.28−0.32). By utilizing forward recursive feature selection model, the number of predictors was reduced from 24 to 9, enhancing model performance for direct approach (R2 of 0.35), with no improvement for indirect approach (R2 of 0.28). The mean SOCD of the French topsoil was 5.29 and 6.14 ​kg ​m−2 by direct and indirect approaches, resulting in SOC stock of 2.8 and 3.3 ​Pg, respectively. We found that the direct approach clearly underestimated the high SOCD (>9 ​kg ​m−2), while the indirect approach performed much better for high SOCD. Our findings serve as a valuable reference for SOCD mapping, thereby providing a scientific basis for maintaining soil health.

Soils are at the crossroads of many existential issues that humanity is currently facing. Soils are a finite resource that is under threat, mainly due to human pressure. There is an urgent need to map and monitor them at field, regional, and global scales in order to improve their management and prevent their degradation. This remains a challenge due to the high and often complex spatial variability inherent to soils. Over the last four decades, major research efforts in the field of pedometrics have led to the development of methods allowing to capture the complex nature of soils. As a result, digital soil mapping (DSM) approaches have been developed for quantifying soils in space and time. DSM and monitoring have become operational thanks to the harmonization of soil databases, advances in spatial modeling and machine learning, and the increasing availability of spatiotemporal covariates, including the exponential increase in freely available remote sensing (RS) data. The latter boosted research in DSM, allowing the mapping of soils at high resolution and assessing the changes through time. We present a review of the main contributions and developments of French (inter)national research, which has a long history in both RS and DSM. Thanks to the French SPOT satellite constellation that started in the early 1980s, the French RS and soil research communities have pioneered DSM using remote sensing. This review describes the data, tools, and methods using RS imagery to support the spatial predictions of a wide range of soil properties and discusses their pros and cons. The review demonstrates that RS data are frequently used in soil mapping (i) by considering them as a substitute for analytical measurements, or (ii) by considering them as covariates related to the controlling factors of soil formation and evolution. It further highlights the great potential of RS imagery to improve DSM, and provides an overview of the main challenges and prospects related to digital soil mapping and future sensors. This opens up broad prospects for the use of RS for DSM and natural resource monitoring.

Satellite-based soil organic carbon content (SOC) mapping over wide regions is generally hampered by the low soil sampling density and the diversity of soil sampling periods. Some unfavorable topsoil conditions, such as high moisture, rugosity, the presence of crop residues, the limited amplitude of SOC values and the limited area of bare soil when a single image is used, are also among the influencing factors. To generate a reliable SOC map, this study addresses the use of Sentinel-2 (S2) temporal mosaics of bare soil (S2Bsoil) over 6 years jointly with soil moisture products (SMPs) derived from Sentinel 1 and 2 images, SOC measurement data and other environmental covariates derived from digital elevation models, lithology maps and airborne gamma-ray data. In this study, we explore (i) the dates and periods that are preferable to construct temporal mosaics of bare soils while accounting for soil moisture and soil management; (ii) which set of covariates is more relevant to explain the SOC variability. From four sets of covariates, the best contributing set was selected, and the median SOC content along with uncertainty at 90% prediction intervals were mapped at a 25-m resolution from quantile regression forest models. The accuracy of predictions was assessed by 10-fold cross-validation, repeated five times. The models using all the covariates had the best model performance. Airborne gamma-ray thorium, slope and S2 bands (e.g., bands 6, 7, 8, 8a) and indices (e.g., calcareous sedimentary rocks, “calcl”) from the “late winter–spring” time series were the most important covariates in this model. Our results also indicated the important role of neighboring topographic distances and oblique geographic coordinates between remote sensing data and parent material. These data contributed not only to optimizing SOC mapping performance but also provided information related to long-range gradients of SOC spatial variability, which makes sense from a pedological point of view.

Soil Security includes dimensions, soil capability, soil condition, soil capital, soil connectivity and soil codification (the “five C's”). This article provides a short review on how soil mapping, digital soil mapping and soil monitoring systems (SM, DSM and SMS) over large areas contribute to these five C's at scales ranging from country to globe. Changes and the evolution in aims of SM, DSM and SMS were driven both by main issues related to policy priorities and associated advances in science and technology. This review shows that SM, DSM and SMS can provide the basis for assessing soil capability and condition over large areas, especially if we assume that capability mainly depends on rather stable soil attributes. Repeated DSM or SMS are appropriated tool to monitor changes in soil condition at these scales. They may even allow mapping changes in soil capability. However, broad-scale SM, DSM and SMS have not yet fully achieved the provision of information concerning the delivery of some soil functions and soil-based ecosystem services. Although significant progress in estimating the capital dimension of soil security has been achieved, there is need to progress monitoring changes in soil capital. Broad-scale SM, DSM and SMS has great potential to increase soil connectivity. The main challenge is adapting our language and our communication to the target audience. There are encouraging initiatives to enhance soil codification. Codification issues are largely driven by the political agenda, there is still an urgent need to increase soil connectivity, especially towards citizens, NGOs and policy-makers.

The spatial assessment of soil organic carbon (SOC) is a major environmental challenge, notably for evaluating soil carbon stocks. Recent works have shown the capability of Sentinel-2 to predict SOC content over temperate agroecosystems characterized with annual crops. However, because spectral models are only applicable on bare soils, the mapping of SOC is often obtained on limited areas. A possible improvement for increasing the number of pixels on which SOC can be retrieved by inverting bare soil reflectance spectra, consists of using optical images acquired at several dates. This study compares different approaches of Sentinel–2 images temporal mosaicking to produce a composite multi-date bare soil image for predicting SOC content over agricultural topsoils. A first approach for temporal mosaicking was based on a per-pixel selection and was driven by soil surface characteristics: bare soil or dry bare soil with/without removing dry vegetation. A second approach for creating composite images was based on a per-date selection and driven either by the models performance from single-date, or by average soil surface indicators of bare soil or dry bare soil. To characterize soil surface, Sentinel-1 (S1)-derived soil moisture and/or spectral indices such as normalized difference vegetation index (NDVI), Normalized Burn Ratio 2 (NBR2), bare soil index (BSI) and a soil surface moisture index (S2WI) were used either separately or in combination. This study highlighted the following results: i) none of the temporal mosaic images improved model performance for SOC prediction compared to the best single-date image; ii) of the per-pixel approaches, temporal mosaics driven by the S1-derived moisture content, and to a lesser extent, by NBR2 index, outperformed the mosaic driven by the BSI index but they did not increase the bare soil area predicted; iii) of the per-date approaches, the best trade-off between predicted area and model performance was achieved from the temporal mosaic driven by the S1-derived moisture content (R2 ~ 0.5, RPD ~ 1.4, RMSE ~ 3.7 g.kg-1) which enabled to more than double (*2.44) the predicted area. This study suggests that a number of bare soil mosaics based on several indicators (moisture, bare soil, roughness…), preferably in combination, might maintain acceptable accuracies for SOC prediction whilst extending over larger areas than single-date images.

There is a need to update soil maps and monitor soil organic carbon (SOC) in the upper horizons or plough layer for enabling decision support and land management, while complying with several policies, especially those favoring soil carbon storage. This review paper is dedicated to the satellite-based spectral approaches for SOC assessment that have been achieved from several satellite sensors, study scales and geographical contexts in the past decade. Most approaches relying on pure spectral models have been carried out since 2019 and have dealt with temperate croplands in Europe, China and North America at the scale of small regions, of some hundreds of km2: dry combustion and wet oxidation were the analytical determination methods used for 50% and 35% of the satellite-derived SOC studies, for which measured topsoil SOC contents mainly referred to mineral soils, typically cambisols and luvisols and to a lesser extent, regosols, leptosols, stagnosols and chernozems, with annual cropping systems with a SOC value of ~15 g·kg−1 and a range of 30 g·kg−1 in median. Most satellite-derived SOC spectral prediction models used limited preprocessing and were based on bare soil pixel retrieval after Normalized Difference Vegetation Index (NDVI) thresholding. About one third of these models used partial least squares regression (PLSR), while another third used random forest (RF), and the remaining included machine learning methods such as support vector machine (SVM). We did not find any studies either on deep learning methods or on all-performance evaluations and uncertainty analysis of spatial model predictions. Nevertheless, the literature examined here identifies satellite-based spectral information, especially derived under bare soil conditions, as an interesting approach that deserves further investigations. Future research includes considering the simultaneous analysis of imagery acquired at several dates i.e., temporal mosaicking, testing the influence of possible disturbing factors and mitigating their effects fusing mixed models incorporating non-spectral ancillary information.

Les sols forestiers sont le plus généralement pauvres et la moitié d’entre eux a une valeur de pHeau inférieure à 5 L’écosystème s’est lentement adapté à cet état de fait via le processus complexe des cycles biogéochimiques, où le recyclage massif des éléments utiles conduit à une frugalité apparente des peuplements forestiers et à un ensemble de fonctions très satisfaisantes, dont la production Toute réduction de fertilité dans ses composantes physique, chimique et biologique, totalement interactives, se traduit par une dégradation de ces fonctions La contrainte physique liée à la mécanisation des opérations sylvicoles présente un risque majeur quant à la durabilité des sols Les effets du tassement sont analysés dans cet article à partir des résultats obtenus dans deux sites expérimentaux installés sur les sols sensibles du plateau Lorrain, suivis pendant une douzaine d’années Les résultats montrent que si la dégradation des sols est rapide, leur restauration est lente Des travaux sur le long terme sont indispensables Quoi qu’il en soit, la protection des sols, en particulier de leur structure physique, représente un enjeu majeur.

In agronomy, soil organic carbon (SOC) content is important for the development and growth of crops. From an environmental monitoring viewpoint, SOC sequestration is essential for mitigating the emission of greenhouse gases into the atmosphere. SOC dynamics in cropland soils should be further studied through various approaches including remote sensing. In order to predict SOC content over croplands in southwestern France (area of 22,177 km²), this study addresses (i) the influence of the dates on which Sentinel-2 (S2) images were acquired in the springs of 2017–2018 as well as the influence of the soil sampling period of a set of samples collected between 2005 and 2018, (ii) the use of soil moisture products (SMPs) derived from Sentinel-1/2 satellites to analyze the influence of surface soil moisture on model performance when included as a covariate, and (iii) whether the spatial distribution of SOC as mapped using S2 is related to terrain-derived attributes. The influences of S2 image dates and soil sampling periods were analyzed for bare topsoil. The dates of the S2 images with the best performance (RPD ≥ 1.7) were 6 April and 26 May 2017, using soil samples collected between 2016 and 2018. The soil sampling dates were also analyzed using SMP values. Soil moisture values were extracted for each sample and integrated into partial least squares regression (PLSR) models. The use of soil moisture as a covariate had no effect on the prediction performance of the models; however, SMP values were used to select the driest dates, effectively mapping topsoil organic carbon. S2 was able to predict high SOC contents in the specific soil types located on the old terraces (mesas) shaped by rivers flowing from the southwestern Pyrénées.

In the interesting paper “Non-Flat Earth Recalibrated for Terrain and Topsoil” published in Soil Systems [...]

Although numerous studies have demonstrated the key role of bacterial diversity in soil functions and ecosystem services, little is known about the variations and determinants of such diversity on a nationwide scale. The overall objectives of this study were i) to describe the bacterial taxonomic richness variations across France, ii) to identify the ecological processes (i.e. selection by the environment and dispersal limitation) influencing this distribution, and iii) to develop a statistical predictive model of soil bacterial richness. We used the French Soil Quality Monitoring Network (RMQS), which covers all of France with 2,173 sites. The soil bacterial richness (i.e. OTU number) was determined by pyrosequencing 16S rRNA genes and related to the soil characteristics, climatic conditions, geomorphology, land use and space. Mapping of bacterial richness revealed a heterogeneous spatial distribution, structured into patches of about 111km, where the main drivers were the soil physico-chemical properties (18% of explained variance), the spatial descriptors (5.25%, 1.89% and 1.02% for the fine, medium and coarse scales, respectively), and the land use (1.4%). Based on these drivers, a predictive model was developed, which allows a good prediction of the bacterial richness (R2adj of 0.56) and provides a reference value for a given pedoclimatic condition.

[This corrects the article DOI: 10.1371/journal.pone.0186766.].

The spatial assessment of soil organic carbon (SOC) is a major environmental challenge, notably for evaluating soil carbon stocks. Recent works have shown the capability of Sentinel-2 optical data to predict SOC content over temperate agroecosystems characterized by annual crops, using a single acquisition date. Considering a Sentinel-2 time series, this work intends to analyze the impact of acquisition date, and related weather and soil surface conditions on the prediction performance of topsoil SOC content (plough layer). A Sentinel-2 time-series was gathered, comprised of the dates corresponding to both the maximum of bare soil coverage and minimum of cloud coverage. Cross-validated partial least squares regression (PLSR) models were constructed between soil reflectance image spectra, and SOC content analyzed from 329 top soil samples collected over the study area. Cross-validation R2 ranged from 0.005 to 0.58, root mean square error from 5.86 to 3.02 g·kg−1 and residual prediction deviation values from 1.0 to 1.5 (without unit), according to date. The main factors influencing these differences were soil roughness, in conjunction with soil moisture, and the cloud and cloud shadow cover of the entire tile. The best performing dates were spring dates characterized by both lowest soil surface roughness and moisture content. Normalized difference vegetation index (NDVI) values below 0.35 did not influence prediction performance. This consolidates the previous results obtained during single date acquisitions and offers wider perspectives for the further use of Sentinel-2 into multidate mosaics for digital soil mapping.

Spatial scaling of microorganisms has been demonstrated over the last decade. However, the processes and environmental filters shaping soil microbial community structure on a broad spatial scale still need to be refined and ranked. Here, we compared bacterial and fungal community composition turnovers through a biogeographical approach on the same soil sampling design at a broad spatial scale (area range: 13300 to 31000 km2): i) to examine their spatial structuring; ii) to investigate the relative importance of environmental selection and spatial autocorrelation in determining their community composition turnover; and iii) to identify and rank the relevant environmental filters and scales involved in their spatial variations. Molecular fingerprinting of soil bacterial and fungal communities was performed on 413 soils from four French regions of contrasting environmental heterogeneity (Landes

The soil variability that we evidenced within the Pomerol vineyard (first part) in France (near to Bordeaux) led us to compare the behaviour of Merlot noir, which is the main local cultivar in this area. This study was conducted on four sites, which belong to the main soil map units of the area. Soils (FAO) were: a deep (A) and a shallow (C) planosols with abrupt textural changes between uppest sandy-gravelly horizons and sandy clay (A) or heavy clay (C) deeper layers, a very sandy well-drained neoluvisol (B), and a very stony cambisol with a temporary watertable (D). Water uptake by vines was assessed by leaf water potential measurements undertaken from June to September. Both observations on shoot elongation and leaf water potential measurements were performed together. The year 1995 exhibited a mild winter, then followed by both temperature and insolation higher than mean values. The rainfall in summer was less than the mean value, and the climatic water balance was negative. As a consequence, the vine phenology exhibited a very early development. Various water behaviours were observed amongst the four experimental plots: some were strongly linked to c1imatic events whereas others showed regulating mechanisms attributable to inherent soil properties such as texture and structure. The vine on soil D exhibited the greatest variations in water potential, showing significant periods of droughtiness during summer. On this soil, the variations in leaf water potential were strongly linked to rainfall events in summer. The vine on soil B kept the less negative values of water potential in relation to the very deep root development which was observed in this light and well-drained sandy material. The vine on soil A had a high water uptake during the period of shoot development, but showed a significant reduction of water availability in august. The most progressive regulation of water uptake was observed on soil C, and could be attributed to the elongation of roots with shrinking during summer in the deep c1ayey horizons. The water behaviour variability was mainly linked both to textural and structural soil properties intluencing soil water retention, aeration, and rooting development. The longest shoots were observed in soil (A) where the elongation speed was the highest. On soil (B) shoot elongation was signiticantly reduced, although water supply was the highest. This was attributed to a low nutrient availability in this very sandy soil. The elongation speed in site (D) was rather chaotic, and was related to marked variations in water supply. The lowest shoot elongation was observed in the shallow planosol (C). Preliminary results on grape quality are showed, and seem to be consistent with these behaviours. These results are consistent with previous studies on various vines of the Bordeaux region which have already shown that various soil properties might induce a control on water behaviour in soil and thus might result in similar consequencies for water availability for vines. The discussion points out the important role played by water uptake control mechanisms on vintage quality in this area.

The Pomerol vineyard (France) is located 35 km east from Bordeaux, on the left side of the Isle river. Its area is approximately 800 ha. The geology is made of a system of fluvial terraces, including gravels and stones of various sizes. The underlying substratum is made of tertiary clays and sands. This stratification of terraces of various thickness leads to overlays of very different materials in which various soil development are observed. We studied the distribution of soils and produced a 1/25 000 soil map showing four main soil scapes and 11 soil maping units. The four main soil scapes were : a plateau, mainly characterised by the presence of swelling clays ; a large area (south and west) made of filtering sands on underlying more clayey materials ; the lower terraces with deep sandy soils ; and areas of immature soils from recent colluvial or alluvial origin. The soil survey pointed out the great variability of soils within the Pomerol vineyard. The textural properties exhibited a very broad range from very sandy to heavy clayey ones, with abrupt changes, both laterally and vertically. These various textural changes might induce various water behaviours. All basic soil data were stored into a geographical information system. Besides its interest for map production, this computerization enables a better characterization of mapping units variability. Actually, from a soil map user point of view, the range of variation - and even the statistical distribution - of a given parameter is as important as the mean observed value. This study gives an exarnple of spatial output taking into account this variability. These georeferenced data will also allow the overlay with other sources of geographical information.

Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen® method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affected by length polymorphism and may provide sufficiently small targets, a crucial criterion for enhancing accuracy and reproducibility of the detection technique. An in silico analysis of 33 primer sets targeting the 18S rRNA gene was performed to select the primer set with the best potential for real-time Q-PCR: short amplicon length; good fungal specificity and coverage. The best consensus between specificity, coverage and amplicon length among the 33 sets tested was the primer set FR1/FF390. This in silico analysis of the specificity of FR1/FF390 also provided additional information to the previously published analysis on this primer set. The specificity of the primer set FR1/FF390 for Fungi was validated in vitro by cloning--sequencing the amplicons obtained from a real time Q-PCR assay performed on five independent soil samples. This assay was also used to evaluate the sensitivity and reproducibility of the method. Finally, fungal abundance in samples from 24 soils with contrasting physico-chemical and environmental characteristics was examined and ranked to determine the importance of soil texture, organic carbon content, C∶N ratio and land use in determining fungal abundance in soils.