18 results on '"Dominguez GA"'
Search Results
2. Incidental finding of rare hemoglobin: hemoglobin Bari in northeast Spain
- Author
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Lahoz Alonso Raquel, Romero Sánchez Naiara, González Sánchez Ruth, Escobar Medina Antonia, López Martos Aurora M., Domínguez García Marta, Beneitez Pastor David, Prieto Grueso Montserrat, Blanco Álvarez Adoración, Urban Giralt Susana, and Esteve Alcalde Patricia
- Subjects
hba1c ,hemoglobin bari ,hplc ,Medical technology ,R855-855.5 - Abstract
Cation exchange high-performance liquid chromatography (HPLC) is one of the techniques available for determining glycated hemoglobin (HbA1c) and also the method of choice for structural hemoglobinopathies screening. The objective of this case is to show how in a routine HbA1c test it is possible to incidentally find a hemoglobinopathy.
- Published
- 2023
- Full Text
- View/download PDF
3. Depressive and Anxious Symptoms Increase with Problematic Technologies Use Among Adults: The Effects of Personal Factors Related to Health Behavior
- Author
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Méndez-López F, Oliván-Blázquez B, Domínguez García M, López-Del-Hoyo Y, Tamayo-Morales O, and Magallón-Botaya R
- Subjects
depression ,anxiety ,problematic information and communication technologies use ,technology addiction ,personal health factors ,Psychology ,BF1-990 ,Industrial psychology ,HF5548.7-5548.85 - Abstract
Fátima Méndez-López,1,2 Bárbara Oliván-Blázquez,1– 3 Marta Domínguez García,1,2,4 Yolanda López-Del-Hoyo,2,3,5 Olaya Tamayo-Morales,2,6 Rosa Magallón-Botaya1,2,7 1Primary Care Research Unit (GAIAP), Health Research Institute of Aragon (IISA), Zaragoza, Spain; 2Network for Research on Chronicity, Primary Care and Health Promotion (RICAPPS), Health Institute Carlos III, Madrid, Spain; 3Department of Psychology and Sociology, University of Zaragoza, Zaragoza, Spain; 4Aragonese Healthcare Service (SALUD), Zaragoza, Spain; 5Mental Health Research in Primary Care Unit, Health Research Institute of Aragon (IISA), Zaragoza, Spain; 6Primary Care Research Unit of Salamanca (APISAL), Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain; 7Department of Medicine, Psychiatry and Dermatology, University of Zaragoza, Zaragoza, SpainCorrespondence: Bárbara Oliván-Blázquez, Primary care research unit, Health Research Institute of Aragon, 13 San Juan Bosco AVE FL 0, Zaragoza, 50009, Spain, Tel +34 976 50 65 78, Email bolivan@unizar.esBackground: Depression and anxiety disorders are a significant and growing health problem that has a significant impact on psychosocial functioning and quality of life. The onset and severity of mental health problems have been related to various biological, psychosocial, and behavioral variables.Purpose: The purpose of this study was to explore the association among the severity of depression and anxiety, problematic information and communications technology (ICT) use, and some related personal factors with health behavior among adults. It also analyzes the moderating role of personal factors in the relationship between the problematic use of ICT and anxiety and depression.Patients and Methods: Descriptive, bivariate, multivariate and moderation analyzes of data from 391 participants of 35– 74 years old in primary health care centers located in Aragón (Spain) were performed between July 2021 and July 2022. The primary outcome was the severity of depressive and anxious symptoms as continuous variable.Results: Low sense of coherence (β = − 0.058; p = 0.043), low self-esteem (β = − 0.171; p=0.002), and low self-efficacy (β = − 0.122; p= 0.001), are predictors of having more severe depressive symptoms. Furthermore, low self-esteem (β = − 0.120; p= 0.012), low self-efficacy (β = − 0.092; p=0.004), and high problematic use of ICT (β = 0.169; p = 0.001), are predictors of having more severe anxiety symptoms. Moderation analyzes were significant in the effect of self-efficacy (b = − 0.040, p=0.001) and resilience (b = − 0.024, p=0.033) on the relationship between problematic ICT use and anxiety.Conclusion: The problematic use of ICT and personal factors are related to depressive and anxiety symptoms. The interrelationship between problematic ICT use, personal factors, and depression needs to be further explored.Keywords: depression, anxiety, problematic information and communication technologies use, technology addiction, personal health factors
- Published
- 2023
4. Lectin-type oxidized LDL receptor 1 defines a population of polymorphonuclear myeloid-derived suppressor cells in cancer patients
- Author
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Condamine, T, Dominguez, GA, Youn, JI, Kossenkov, A, Mony, S, Alicea-Torres, K, Tsyganov, E, Hashimoto, A, Nefedova, Y, Lin, C, Partlova, S, Garfall, A, Vogl, DT, Xu, X, Knight, SC, Malietzsis, G, Lee, GH, Eruslanov, E, Albelda, SM, Wang, X, Mehta, JL, Bewtra, M, Rustgi, A, Hockstein, N, Witt, R, Masters, G, Nam, B, Smirnov, D, Sepulveda, MA, Gabrilovich, DI, Biotechnology and Biological Sciences Research Cou, and Biotechnology and Biological Sciences Research Council
- Abstract
Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) are important regulators of immune responses in cancer and have been directly implicated in the promotion of tumor progression. However, the heterogeneity of these cells and the lack of distinct markers hamper the progress in understanding the biology and clinical importance of these cells. Using partial enrichment of PMN-MDSC with gradient centrifugation, we determined that low-density PMN-MDSC and high-density neutrophils from the same cancer patients had a distinct gene profile. The most prominent changes were observed in the expression of genes associated with endoplasmic reticulum (ER) stress. Unexpectedly, low-density lipoprotein (LDL) was one of the most increased regulators, and its receptor oxidized LDL receptor 1 (OLR1) was one of the most overexpressed genes in PMN-MDSC. Lectin-type oxidized LDL receptor-1 (LOX-1) encoded by OLR1 was practically undetectable in neutrophils in peripheral blood of healthy donors, whereas 5 to 15% of total neutrophils in cancer patients and 15 to 50% of neutrophils in tumor tissues were LOX-1+. In contrast to their LOX-1− counterparts, LOX-1+ neutrophils had gene signature, potent immunosuppressive activity, up-regulation of ER stress, and other biochemical characteristics of PMN-MDSCs. Moreover, induction of ER stress in neutrophils from healthy donors up-regulated LOX-1 expression and converted these cells to suppressive PMN-MDSCs. Thus, we identified a specific marker of human PMN-MDSC associated with ER stress and lipid metabolism, which provides new insights into the biology and potential therapeutic targeting of these cells.
- Published
- 2016
5. Juventud y educación en Cuba: Estrategia de inclusión social femenina
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Domínguez García, María Isabel
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- 2012
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6. Estrategias en la comunidad autónoma de Cataluña
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Salleras Sanmartí Lluís, Domínguez García Àngela, Batalla i Clavé Joan, and Prats i Coll Ramón
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Medicine ,Public aspects of medicine ,RA1-1270 - Published
- 1999
7. Detecting Prostate Cancer Using Pattern Recognition Neural Networks With Flow Cytometry-Based Immunophenotyping in At-Risk Men.
- Author
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Dominguez GA, Polo AT, Roop J, Campisi AJ, Somer RA, Perzin AD, Gabrilovich DI, and Kumar A
- Abstract
Current screening methods for prostate cancer (PCa) result in a large number of false positives making it difficult for clinicians to assess disease status, thus warranting advancements in screening and early detection methods. The goal of this study was to design a liquid biopsy test that uses flow cytometry-based immunophenotyping and artificial neural network (ANN) analysis to detect PCa. Numerous myeloid and lymphoid cell populations, including myeloid-derived suppressor cells, were measured from 156 patients with PCa, 123 with benign prostatic hyperplasia (BPH), and 99 male healthy donor (HD) controls. Using pattern recognition neural network (PRNN) analysis, a type of ANN, PCa detection compared against HD resulted in 96.6% sensitivity, 87.5% specificity, and an area under the curve (AUC) value of 0.97. Detecting patients with higher risk disease (⩾Gleason 7) against lower risk disease (BPH/Gleason 6) resulted in 92.0% sensitivity, 42.7% specificity, and an AUC of 0.72. This study suggests that analyzing flow cytometry immunophenotyping data with PRNNs may prove to be a useful tool to improve PCa detection and reduce the number of unnecessary prostate biopsies performed each year., Competing Interests: Declaration of Conflicting Interests:The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: G.A.D., A.T.P*, J.R., A.J.C., and A.K. are employees (or former employees*) of Anixa Diagnostics Corp. and have ownership (including patents) interests., (© The Author(s) 2020.)
- Published
- 2020
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8. Unique pattern of neutrophil migration and function during tumor progression.
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Patel S, Fu S, Mastio J, Dominguez GA, Purohit A, Kossenkov A, Lin C, Alicea-Torres K, Sehgal M, Nefedova Y, Zhou J, Languino LR, Clendenin C, Vonderheide RH, Mulligan C, Nam B, Hockstein N, Masters G, Guarino M, Schug ZT, Altieri DC, and Gabrilovich DI
- Subjects
- Aged, Animals, Disease Progression, Female, Humans, Male, Mice, Mice, Inbred C57BL, Middle Aged, Chemotaxis, Leukocyte immunology, Neoplasms immunology, Neoplasms pathology, Neutrophil Infiltration immunology, Neutrophils immunology
- Abstract
Although neutrophils have been linked to the formation of the pre-metastatic niche, the mechanism of their migration to distant, uninvolved tissues has remained elusive. We report that bone marrow neutrophils from mice with early-stage cancer exhibited much more spontaneous migration than that of control neutrophils from tumor-free mice. These cells lacked immunosuppressive activity but had elevated rates of oxidative phosphorylation and glycolysis, and increased production of ATP, relative to that of control neutrophils. Their enhanced spontaneous migration was mediated by autocrine ATP signaling through purinergic receptors. In ectopic tumor models and late stages of cancer, bone marrow neutrophils demonstrated potent immunosuppressive activity. However, these cells had metabolic and migratory activity indistinguishable from that of control neutrophils. A similar pattern of migration was observed for neutrophils and polymorphonuclear myeloid-derived suppressor cells from patients with cancer. These results elucidate the dynamic changes that neutrophils undergo in cancer and demonstrate the mechanism of neutrophils' contribution to early tumor dissemination.
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- 2018
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9. Mercury assessment, macrobenthos diversity and environmental quality conditions in the Salado Estuary (Gulf of Guayaquil, Ecuador) impacted by anthropogenic influences.
- Author
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Calle P, Monserrate L, Medina F, Calle Delgado M, Tirapé A, Montiel M, Ruiz Barzola O, Cadena OA, Dominguez GA, and Alava JJ
- Subjects
- Animals, Biodiversity, Bivalvia classification, Ecuador, Geologic Sediments chemistry, Seawater chemistry, Bivalvia drug effects, Environmental Monitoring methods, Estuaries, Mercury analysis, Water Pollutants, Chemical analysis, Water Quality
- Abstract
Water and sediment quality, macrobenthos diversity and mercury levels were assessed in the Salado Estuary, Gulf of Guayaquil (Ecuador) during 2008, 2009 and 2014. Severe hypoxia, anoxia and large fluctuations of salinity occurred in an impacted sector within Guayaquil city relative to a mangrove area within the Salado Mangroves Faunal Production Reserve. Significant inter-site and temporal differences were observed for dissolved oxygen, salinity, total dissolved solids, percentage of silts and clays, and species diversity. Macrobenthos' species richness for both sectors was greater during 2008. Sediments revealed high concentrations of total mercury (THg) (1.20-2.76 mg kg
-1 dw), exceeding Ecuador's SQG (0.1 mg kg-1 dw). Sediment THg were significantly lower in 2014 than 2008/09. Biota sediment sccumulation factor values for mussels (3.0 to 34), indicate high bioaccumulation potential from mercury-contaminated sediments. This work highlights the need to develop stronger environmental policies to protect the Salado Estuary from anthropogenic stressors., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2018
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10. CD38+ M-MDSC expansion characterizes a subset of advanced colorectal cancer patients.
- Author
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Karakasheva TA, Dominguez GA, Hashimoto A, Lin EW, Chiu C, Sasser K, Lee JW, Beatty GL, Gabrilovich DI, and Rustgi AK
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- ADP-ribosyl Cyclase 1 immunology, Adult, Aged, Animals, Antibodies, Monoclonal, Colorectal Neoplasms immunology, Esophageal Neoplasms immunology, Female, Humans, Immunosuppressive Agents pharmacology, Lymphocytes, Male, Membrane Glycoproteins immunology, Mice, Middle Aged, Monocytes, Pennsylvania, ADP-ribosyl Cyclase 1 metabolism, Colorectal Neoplasms metabolism, Esophageal Neoplasms metabolism, Leukocytes, Mononuclear metabolism, Membrane Glycoproteins metabolism, Myeloid-Derived Suppressor Cells metabolism, Neutrophils metabolism
- Abstract
Background: Myeloid-derived suppressor cells (MDSCs) are a population of immature immune cells with several protumorigenic functions. CD38 is a transmembrane receptor-ectoenzyme expressed by MDSCs in murine models of esophageal cancer. We hypothesized that CD38 could be expressed on MDSCs in human colorectal cancer (CRC), which might allow for a new perspective on therapeutic targeting of human MDSCs with anti-CD38 monoclonal antibodies in this cancer., Methods: Blood samples were collected from 41 CRC patients and 8 healthy donors, followed by peripheral blood mononuclear cell (PBMC) separation. Polymorphonuclear (PMN-) and monocytic (M-) MDSCs and CD38 expression levels were quantified by flow cytometry. The immunosuppressive capacity of M-MDSCs from 10 CRC patients was validated in a mixed lymphocyte reaction (MLR) assay., Results: A significant expansion of CD38+ M-MDSCs and a trend of expansion of CD38+ PMN-MDSCs (accompanied by a trend of increased CD38 expression on both M- and PMN-MDSCs) were observed in PBMCs of CRC patients when compared with healthy donors. The CD38+ M-MDSCs from CRC patients were found to be immunosuppressive when compared with mature monocytes. CD38+ M- and PMN-MDSC frequencies were significantly higher in CRC patients who previously received treatment when compared with treatment-naive patients., Conclusions: This study provides a rationale for an attempt to target M-MDSCs with an anti-CD38 monoclonal antibody in metastatic CRC patients., Funding: NCI P01-CA14305603, the American Cancer Society, Scott and Suzi Lustgarten Family Colon Cancer Research Fund, Hansen Foundation, and Janssen Research and Development.
- Published
- 2018
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11. Selective Targeting of Myeloid-Derived Suppressor Cells in Cancer Patients Using DS-8273a, an Agonistic TRAIL-R2 Antibody.
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Dominguez GA, Condamine T, Mony S, Hashimoto A, Wang F, Liu Q, Forero A, Bendell J, Witt R, Hockstein N, Kumar P, and Gabrilovich DI
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- Adult, Aged, Aged, 80 and over, Antibodies, Monoclonal administration & dosage, Disease-Free Survival, Humans, Immunosuppression Therapy, Lymphocyte Activation drug effects, Lymphocyte Activation immunology, Middle Aged, Monocytes immunology, Myeloid Cells immunology, Myeloid-Derived Suppressor Cells immunology, Neoplasms immunology, Receptors, TNF-Related Apoptosis-Inducing Ligand agonists, Immunotherapy, Myeloid-Derived Suppressor Cells drug effects, Neoplasms drug therapy, Receptors, TNF-Related Apoptosis-Inducing Ligand immunology
- Abstract
Purpose: Myeloid-derived suppressor cells (MDSC) are one of the major contributors to immune suppression in cancer. We recently have demonstrated in preclinical study that MDSCs are sensitive to TRAIL receptor 2 (TRAIL-R2) agonist. The goal of this study was to clinically test the hypothesis that targeting TRAIL-R2 can selectively eliminate MDSCs. Experimental Design: The TRAIL-R2 agonistic antibody (DS-8273a) has been tested in 16 patients with advanced cancers enrolled in a phase I trial. The antibody (24 mg/kg) was administered intravenously once every 3 weeks till disease progression, unacceptable toxicities, or withdrawal of consent. The safety and the presence of various populations of myeloid and lymphoid cells in peripheral blood and tumor tissues were evaluated. Results: The treatment was well tolerated with only mild to moderate adverse events attributable to the study drug. Treatment with DS-8273a resulted in reduction of the elevated numbers of MDSCs in the peripheral blood of most patients to the levels observed in healthy volunteers. However, in several patients, MDSCs rebounded back to the pretreatment level by day 42. In contrast, DS-8273a did not affect the number of neutrophils, monocytes, and other populations of myeloid and lymphoid cells. Decrease in MDSCs inversely correlated with the length of progression-free survival. In tumors, DS-8273a treatment resulted in a decrease of MDSCs in 50% of the patients who were able to provide pre- and on-treatment biopsies. Conclusions: Targeting TRAIL-R2 resulted in elimination of different populations of MDSCs without affecting mature myeloid or lymphoid cells. These data support the use of this antibody in combination immmunotherapy of cancer. Clin Cancer Res; 23(12); 2942-50. ©2016 AACR ., (©2016 American Association for Cancer Research.)
- Published
- 2017
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12. Measurement of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry.
- Author
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Dominguez GA, Perlo J, Fraenza CC, and Anoardo E
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- Cholesterol chemistry, Dimyristoylphosphatidylcholine chemistry, Elastic Modulus, Magnetic Resonance Spectroscopy methods, Unilamellar Liposomes chemistry
- Abstract
The elastic properties of lipid membranes can be conveniently characterized through the bending elastic modulus κ. Elasticity directly affects the deformability of a membrane, morphological and shape transitions, fusion, lipid-protein interactions, etc. It is also a critical property for the formulation of ultradeformable liposomes, and of interest for the design of theranostic liposomes for efficient drug delivery systems and/or different imaging contrast agents. Measurements of κ in liposome membranes have been made using the fast field cycling nuclear magnetic relaxometry technique. We analyze the capability of the technique to provide a consistent value of the measured quantity under certain limiting conditions. Relaxation dispersions were measured acquiring a minimal quantity of points, within a reduced Larmor frequency range and, under inferior experimental conditions (in the presence of magnetic field in-homogeneity and lower power supply stability). A simplified model is discussed, showing practical advantages when fitting the data within the reduced frequency range. Experiments are contrasted with standard measurements performed in a state-of-the-art relaxometer. The methodology was tested in samples of 1,2-dimyristoyl-sn-glycero-3-phosphocholine with different percentiles of cholesterol. We observe a tendency to a decrease in κ with increasing temperature, and a tendency to increase with the cholesterol percentile., (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2016
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13. Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients.
- Author
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Condamine T, Dominguez GA, Youn JI, Kossenkov AV, Mony S, Alicea-Torres K, Tcyganov E, Hashimoto A, Nefedova Y, Lin C, Partlova S, Garfall A, Vogl DT, Xu X, Knight SC, Malietzis G, Lee GH, Eruslanov E, Albelda SM, Wang X, Mehta JL, Bewtra M, Rustgi A, Hockstein N, Witt R, Masters G, Nam B, Smirnov D, Sepulveda MA, and Gabrilovich DI
- Abstract
Polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) are important regulators of immune responses in cancer and have been directly implicated in promotion of tumor progression. However, the heterogeneity of these cells and lack of distinct markers hampers the progress in understanding of the biology and clinical importance of these cells. Using partial enrichment of PMN-MDSC with gradient centrifugation we determined that low density PMN-MDSC and high density neutrophils from the same cancer patients had a distinct gene profile. Most prominent changes were observed in the expression of genes associated with endoplasmic reticulum (ER) stress. Surprisingly, low-density lipoprotein (LDL) was one of the most increased regulators and its receptor oxidized LDL receptor 1 OLR1 was one of the most overexpressed genes in PMN-MDSC. Lectin-type oxidized LDL receptor 1 (LOX-1) encoded by OLR1 was practically undetectable in neutrophils in peripheral blood of healthy donors, whereas 5-15% of total neutrophils in cancer patients and 15-50% of neutrophils in tumor tissues were LOX-1
+ . In contrast to their LOX-1- counterparts, LOX-1+ neutrophils had gene signature, potent immune suppressive activity, up-regulation of ER stress, and other biochemical characteristics of PMN-MDSC. Moreover, induction of ER stress in neutrophils from healthy donors up-regulated LOX-1 expression and converted these cells to suppressive PMN-MDSC. Thus, we identified a specific marker of human PMN-MDSC associated with ER stress and lipid metabolism, which provides new insight to the biology and potential therapeutic targeting of these cells.- Published
- 2016
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14. Effects of charge and surface ligand properties of nanoparticles on oxidative stress and gene expression within the gut of Daphnia magna.
- Author
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Dominguez GA, Lohse SE, Torelli MD, Murphy CJ, Hamers RJ, Orr G, and Klaper RD
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- Animals, Daphnia genetics, Daphnia metabolism, Digestive System metabolism, Female, Gold chemistry, Ligands, Nanoparticles chemistry, Oxidative Stress genetics, Static Electricity, Water metabolism, Water Pollutants, Chemical chemistry, Daphnia drug effects, Digestive System drug effects, Gene Expression drug effects, Gold toxicity, Nanoparticles adverse effects, Oxidative Stress drug effects, Water Pollutants, Chemical toxicity
- Abstract
Concern has been raised regarding the current and future release of engineered nanomaterials into aquatic environments from industry and other sources. However, not all nanomaterials may cause an environmental impact and identifying which nanomaterials may be of greatest concern has been difficult. It is thought that the surface groups of a functionalized nanoparticles (NPs) may play a significant role in determining their interactions with aquatic organisms, but the way in which surface properties of NPs impact their toxicity in whole organisms has been minimally explored. A major point of interaction of NPs with aquatic organisms is in the gastrointestinal tract as they ingest particulates from the water column or from the sediment. The main goal of this study was to use model gold NP (AuNPs) to evaluate the potential effects of the different surfaces groups on NPs on the gut of an aquatic model organism, Daphnia magna. In this study, we exposed daphnids to a range of AuNPs concentrations and assessed the impact of AuNP exposure in the daphnid gut by measuring reactive oxygen species (ROS) production and expression of genes associated with oxidative stress and general cellular stress: glutathione S-transferase (gst), catalase (cat), heat shock protein 70 (hsp70), and metallothionein1 (mt1). We found ROS formation and gene expression were impacted by both charge and the specific surface ligand used. We detected some degree of ROS production in all NP exposures, but positively charged AuNPs induced a greater ROS response. Similarly, we observed that, compared to controls, both positively charged AuNPs and only one negatively AuNP impacted expression of genes associated with cellular stress. Finally, ligand-AuNP exposures showed a different toxicity and gene expression profile than the ligand alone, indicating a NP specific effect., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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15. The direction of migration of T-lymphocytes under flow depends upon which adhesion receptors are engaged.
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Dominguez GA, Anderson NR, and Hammer DA
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- Anisotropy, Cell Adhesion physiology, Cell Movement physiology, Cells, Cultured, Humans, Shear Strength physiology, Blood Flow Velocity physiology, Cell Adhesion Molecules metabolism, Intercellular Adhesion Molecule-1 metabolism, T-Lymphocytes cytology, T-Lymphocytes physiology, Vascular Cell Adhesion Molecule-1 metabolism
- Abstract
T-lymphocyte migration is important for homing, cell trafficking, and immune surveillance. T-lymphocytes express lymphocyte function-associated antigen-1 (LFA-1; αLβ2) and very late antigen-4 (VLA-4; α4β1), which bind to their cognate ligands, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). These adhesive interactions provide T-lymphocytes with the ability to withstand hemodynamic shear forces to facilitate adhesion and migration along the blood endothelium. Recently, it has been shown that T-lymphocytes will crawl upstream against the direction of flow on surfaces functionalized with ICAM-1. Here, we have investigated whether the identity of the receptor and the magnitude of its engagement affects the direction of T-lymphocyte migration under flow. We used microcontact printed ICAM-1 and VCAM-1 PDMS surfaces on which density and type of adhesion molecule can be tightly controlled and non-specific adhesion adequately blocked. Using a laminar flow chamber, we demonstrate that T-lymphocytes migrate either upstream or downstream dependent upon ligand type, ligand concentration and shear rate. T-lymphocytes were found to migrate upstream on ICAM-1 but downstream on VCAM-1 surfaces - a behavior unique to T-lymphocytes. By varying concentrations of ICAM-1 and VCAM-1, directed migration under flow was observed to be dependent upon the type and concentration of ligand. As shear rates increase, T-lymphocytes favor upstream migration when any ICAM-1 is present, even in the presence of substantial amounts of VCAM-1. Furthermore, a loss of cytoskeletal polarity was observed upon introduction of fluid flow with reorganization that is dependent upon ligand presentation. These results indicate that T-lymphocytes exhibit two different modes of motility - upstream or downstream - under fluid flow that depends on ligand composition and the shear rate.
- Published
- 2015
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16. Control of transcriptional repression of the vitellogenin receptor gene in largemouth bass (Micropterus salmoides) by select estrogen receptors isotypes.
- Author
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Dominguez GA, Bisesi JH Jr, Kroll KJ, Denslow ND, and Sabo-Attwood T
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- Animals, Base Sequence, Bass genetics, Benzhydryl Compounds pharmacology, Binding Sites, Cloning, Molecular, Down-Regulation, Egg Proteins drug effects, Egg Proteins genetics, Estradiol pharmacology, Estrogen Receptor alpha agonists, Estrogen Receptor alpha genetics, Estrogen Receptor beta agonists, Estrogen Receptor beta genetics, Ethinyl Estradiol pharmacology, Fish Proteins drug effects, Fish Proteins genetics, Genes, Reporter, HEK293 Cells, Humans, Insulin pharmacology, Molecular Sequence Data, Phenols pharmacology, Promoter Regions, Genetic, RNA, Messenger metabolism, Receptors, Cell Surface drug effects, Receptors, Cell Surface genetics, Sp1 Transcription Factor metabolism, Transfection, Bass metabolism, Egg Proteins metabolism, Estrogen Receptor alpha metabolism, Estrogen Receptor beta metabolism, Fish Proteins metabolism, Receptors, Cell Surface metabolism, Transcription, Genetic drug effects
- Abstract
The vitellogenin receptor (Vtgr) plays an important role in fish reproduction. This receptor functions to incorporate vitellogenin (Vtg), a macromolecule synthesized and released from the liver in the bloodstream, into oocytes where it is processed into yolk. Although studies have focused on the functional role of Vtgr in fish, the mechanistic control of this gene is still unexplored. Here we report the identification and analysis of the first piscine 5' regulatory region of the vtgr gene which was cloned from largemouth bass (Micropterus salmoides). Using this putative promoter sequence, we investigated a role for hormones, including insulin and 17β-estradiol (E2), in transcriptional regulation through cell-based reporter assays. No effect of insulin was observed, however, E2 was able to repress transcriptional activity of the vtgr promoter through select estrogen receptor subtypes, Esr1 and Esr2a but not Esr2b. Electrophoretic mobility shift assay demonstrated that Esr1 likely interacts with the vtgr promoter region through half ERE and/or SP1 sites, in part. Finally we also show that ethinylestradiol (EE2), but not bisphenol-A (BPA), represses promoter activity similarly to E2. These results reveal for the first time that the Esr1 isoform may play an inhibitory role in the expression of LMB vtgr mRNA under the influence of E2, and potent estrogens such as EE2. In addition, this new evidence suggests that vtgr may be a target of select endocrine disrupting compounds through environmental exposures., (© The Author 2014. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2014
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17. Effect of adhesion and chemokine presentation on T-lymphocyte haptokinesis.
- Author
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Dominguez GA and Hammer DA
- Subjects
- Animals, Chemokine CCL19 metabolism, Chemokine CCL21 metabolism, Dose-Response Relationship, Drug, Homeostasis, Humans, Immunoglobulin G chemistry, Intercellular Adhesion Molecule-1 metabolism, Ligands, Mice, Protein Binding, Protein Structure, Tertiary, Receptors, CCR7 metabolism, T-Lymphocytes immunology, Vascular Cell Adhesion Molecule-1 metabolism, Cell Adhesion, Cell Movement, Chemokines metabolism, T-Lymphocytes cytology
- Abstract
Motility is critical for the function of T-lymphocytes. Motility in T-lymphocytes is driven by the occupancy of chemokine receptors by chemokines, and modulated by adhesive interactions. However, it is not well understood how the combination of adhesion and chemokine binding affects T-lymphocyte migration. We used microcontact printing on polymeric substrates to measure how lymphocyte migration is quantitatively controlled by adhesion and chemokine ligation. Focusing only on random motion, we found that T-lymphocytes exhibit biphasic motility in response to the substrate concentration of either ICAM-1 or VCAM-1, and generally display more active motion on ICAM-1 surfaces. Furthermore, we examined how the combination of the homeostatic chemokines CCL19 and CCL21 contribute to motility. By themselves, CCL19 and CCL21, ligands for CCR7, elicit biphasic motility, but their combination synergistically increases CCR7 mediated chemokinesis on ICAM-1. By presenting CCL21 with ICAM-1 on the surface with soluble CCL19, we observed random motion that is greater than what is observed with soluble chemokines alone. These data suggest that ICAM-1 has a greater contribution to motility than VCAM-1 and that both adhesive interactions and chemokine ligation work in concert to control T-lymphocyte motility.
- Published
- 2014
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18. Identification and transcriptional modulation of the largemouth bass, Micropterus salmoides, vitellogenin receptor during oocyte development by insulin and sex steroids.
- Author
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Dominguez GA, Quattro JM, Denslow ND, Kroll KJ, Prucha MS, Porak WF, Grier HJ, and Sabo-Attwood TL
- Subjects
- Amino Acid Sequence, Animals, Cloning, Molecular, Egg Proteins metabolism, Female, Gene Expression Regulation, Developmental drug effects, Molecular Sequence Data, Oocytes metabolism, Oocytes physiology, Oogenesis drug effects, Oogenesis genetics, Phylogeny, Receptors, Cell Surface metabolism, Sequence Homology, Amino Acid, Transcription, Genetic drug effects, Transcription, Genetic physiology, Bass genetics, Bass metabolism, Bass physiology, Egg Proteins genetics, Gonadal Steroid Hormones pharmacology, Insulin pharmacology, Oocytes drug effects, Receptors, Cell Surface genetics
- Abstract
Fish vitellogenin synthesized and released from the liver of oviparous animals is taken up into oocytes by the vitellogenin receptor. This is an essential process in providing nutrient yolk to developing embryos to ensure successful reproduction. Here we disclose the full length vtgr cDNA sequence for largemouth bass (LMB) that reveals greater than 90% sequence homology with other fish vtgr sequences. We classify LMB Vtgr as a member of the low density lipoprotein receptor superfamily based on conserved domains and categorize as the short variant that is devoid of the O-glycan segment. Phylogenetic analysis places LMB Vtgr sequence into a well-supported monophyletic group of fish Vtgr. Real-time PCR showed that the greatest levels of LMB vtgr mRNA expression occurred in previtellogenic ovarian tissues. In addition, we reveal the effects of insulin, 17beta-estradiol (E(2)), and 11-ketotestosterone (11-KT) in modulation of vtgr, esr, and ar mRNAs in previtellogenic oocytes. Insulin increased vtgr expression levels in follicles ex vivo while exposure to E(2) or 11-KT did not result in modulation of expression. However, both steroids were able to repress insulin-induced vtgr transcript levels. Coexposure with insulin and E(2) or of insulin and 11-KT increased ovarian esr2b and ar mRNA levels, respectively, which suggest a role for these nuclear receptors in insulin-mediated signaling pathways. These data provide the first evidence for the ordered stage-specific expression of LMB vtgr during the normal reproductive process and the hormonal influence of insulin and sex steroids on controlling vtgr transcript levels in ovarian tissues.
- Published
- 2012
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