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181 results on '"Domingues, Maria Rosário"'

6. Mapping the lipidome in mitochondria‐associated membranes (MAMs) in an in vitro model of Alzheimer's disease.

Author

Fernandes, Tânia, Melo, Tânia, Conde, Tiago, Neves, Bruna, Domingues, Pedro, Resende, Rosa, Pereira, Cláudia F., Moreira, Paula I., and Domingues, Maria Rosário

Subjects
ALZHEIMER'S disease, UNFOLDED protein response, LIPID metabolism, LIPID synthesis, ORGANELLES, PATHOLOGICAL physiology, MITOCHONDRIA
Abstract

The disruption of mitochondria‐associated endoplasmic reticulum (ER) membranes (MAMs) plays a relevant role in Alzheimer's disease (AD). MAMs have been implicated in neuronal dysfunction and death since it is associated with impairment of functions regulated in this subcellular domain, including lipid synthesis and trafficking, mitochondria dysfunction, ER stress‐induced unfolded protein response (UPR), apoptosis, and inflammation. Since MAMs play an important role in lipid metabolism, in this study we characterized and investigated the lipidome alterations at MAMs in comparison with other subcellular fractions, namely microsomes and mitochondria, using an in vitro model of AD, namely the mouse neuroblastoma cell line (N2A) over‐expressing the APP familial Swedish mutation (APPswe) and the respective control (WT) cells. Phospholipids (PLs) and fatty acids (FAs) were isolated from the different subcellular fractions and analyzed by HILIC‐LC–MS/MS and GC–MS, respectively. In this in vitro AD model, we observed a down‐regulation in relative abundance of some phosphatidylcholine (PC), lysophosphatidylcholine (LPC), and lysophosphatidylethanolamine (LPE) species with PUFA and few PC with saturated and long‐chain FA. We also found an up‐regulation of CL, and antioxidant alkyl acyl PL. Moreover, multivariate analysis indicated that each organelle has a specific lipid profile adaptation in N2A APPswe cells. In the FAs profile, we found an up‐regulation of C16:0 in all subcellular fractions, a decrease of C18:0 levels in total fraction (TF) and microsomes fraction, and a down‐regulation of 9‐C18:1 was also found in mitochondria fraction in the AD model. Together, these results suggest that the over‐expression of the familial APP Swedish mutation affects lipid homeostasis in MAMs and other subcellular fractions and supports the important role of lipids in AD physiopathology. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Fatty Acid Profiling as a Tool for Fostering the Traceability of the Halophyte Plant Salicornia ramosissima and Contributing to Its Nutritional Valorization.

Author

Ricardo, Fernando, Veríssimo, Ana Carolina, Maciel, Elisabete, Domingues, Maria Rosário, and Calado, Ricardo

Subjects
OMEGA-3 fatty acids, HALOPHYTES, GAS chromatography/Mass spectrometry (GC-MS), MARINE algae, CULTIVATED plants, TRUST
Abstract

Salicornia ramosissima, commonly known as glasswort or sea asparagus, is a halophyte plant cultivated for human consumption that is often referred to as a sea vegetable rich in health-promoting n-3 fatty acids (FAs). Yet, the effect of abiotic conditions, such as salinity and temperature, on the FA profile of S. ramosissima remains largely unknown. These factors can potentially shape its nutritional composition and yield unique fatty acid signatures that can reveal its geographical origin. In this context, samples of S. ramosissima were collected from four different locations along the coastline of mainland Portugal and their FAs were profiled through gas chromatography–mass spectrometry. The lipid extracts displayed a high content of essential FAs, such as 18:2n-6 and 18:3n-3. In addition to an epoxide fatty acid exclusively identified in samples from the Mondego estuary, the relative abundance of FAs varied between origin sites, revealing that FA profiles can be used as site-specific lipid fingerprints. This study highlights the role of abiotic conditions on the nutritional profile of S. ramosissima and establishes FA profiling as a potential avenue to trace the geographic origin of this halophyte plant. Overall, the present approach can make origin certification possible, safeguard quality, and enhance consumers' trust in novel foods. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Water-Soluble Saccharina latissima Polysaccharides and Relation of Their Structural Characteristics with In Vitro Immunostimulatory and Hypocholesterolemic Activities

Author

Moreira, Ana S. P., primary, Gaspar, Diana, additional, Ferreira, Sónia S., additional, Correia, Alexandra, additional, Vilanova, Manuel, additional, Perrineau, Marie-Mathilde, additional, Kerrison, Philip D., additional, Gachon, Claire M. M., additional, Domingues, Maria Rosário, additional, Coimbra, Manuel A., additional, Coreta-Gomes, Filipe M., additional, and Nunes, Cláudia, additional

Published
2023
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22. Advances and Challenges in Plant Sterol Research: Fundamentals, Analysis, Applications and Production.

Author

Evtyugin, Dmitry D., Evtuguin, Dmitry V., Casal, Susana, and Domingues, Maria Rosário

Subjects
DRUG delivery systems, SUSTAINABILITY, STEROLS, TERPENES, CHEMICAL structure
Abstract

Plant sterols (PS) are cholesterol-like terpenoids widely spread in the kingdom Plantae. Being the target of extensive research for more than a century, PS have topped with evidence of having beneficial effects in healthy subjects and applications in food, cosmetic and pharmaceutical industries. However, many gaps in several fields of PS's research still hinder their widespread practical applications. In fact, many of the mechanisms associated with PS supplementation and their health benefits are still not fully elucidated. Furthermore, compared to cholesterol data, many complex PS chemical structures still need to be fully characterized, especially in oxidized PS. On the other hand, PS molecules have also been the focus of structural modifications for applications in diverse areas, including not only the above-mentioned but also in e.g., drug delivery systems or alternative matrixes for functional foods and fats. All the identified drawbacks are also superimposed by the need of new PS sources and technologies for their isolation and purification, taking into account increased environmental and sustainability concerns. Accordingly, current and future trends in PS research warrant discussion. [ABSTRACT FROM AUTHOR]

Published
2023
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26. Mapping the Polar Lipidome of Macroalgae using LC‐MS‐Based Approaches for Add‐Value Applications.

Author

Lopes, Diana, Rey, Felisa, Melo, Tânia, P. Moreira, Ana S., Marques, Francisca, Abreu, Maria H., Domingues, Pedro, and Domingues, Maria Rosário

Subjects
BETAINE, GLYCOLIPIDS, LIQUID chromatography-mass spectrometry, MARINE algae, FATTY acid analysis, MARINE art
Abstract

Marine macroalgae are emerging as sustainable and healthy food, and a source of lipids with nutritional and bioactive properties. Nonetheless, the lipidome of macroalgae is little explored compared to other photosynthetic organisms. Most of the literature is devoted to the analysis of fatty acid (FA) from macroalgae, because of they are a source of long‐chain omega‐3 and omega‐6 polyunsaturated FA, which can be used as an alternative to fish oil. However, FA occurs mostly esterified in other lipids, such as polar lipids, that still overlooked. The data published on the study of polar lipids (phospholipids, glycolipids, betaine lipids) pinpoint their remarkable characteristics and bioactive properties which mainly remain unexplored. The lipidome of macroalgae seems to be species‐specific, while some lipid classes appear to be characteristic of phyla. Lipidome plasticity of macroalgae is verified to change under abiotic and biotic factors which may modulate the functionality and add‐value of the biomass. This biochemical plasticity represents an advantage to obtain ingredients for target applications and niche of markets. This review provides an overview on macroalgae lipidome characterization, addressing analysis workflow based on liquid chromatography coupled to mass spectrometry, and discussing the bioactive properties and biotechnological applications of macroalga lipids. Practical Applications: The current state of the art on marine macroalgae lipidomics is reviewed, including the lipid characterization using modern liquid chromatography‐mass spectrometry, the distribution of polar lipids by macroalgae species and phylum, their bioactive properties, and potential biotechnological applications. In a time of increasing awareness of sustainably produced marine resources, the lipids from macroalgae are still underexplored. This review provides information for smart valorization of macroalgae as source of add‐value lipids contributing to the development of blue bioeconomy. [ABSTRACT FROM AUTHOR]

Published
2023
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28. Potential Anti-Obesity, Anti-Steatosis, and Anti-Inflammatory Properties of Extracts from the Microalgae Chlorella vulgaris and Chlorococcum amblystomatis under Different Growth Conditions

Author

Regueiras, Ana, primary, Huguet, Álvaro, additional, Conde, Tiago, additional, Couto, Daniela, additional, Domingues, Pedro, additional, Domingues, Maria Rosário, additional, Costa, Ana Margarida, additional, Silva, Joana Laranjeira da, additional, Vasconcelos, Vitor, additional, and Urbatzka, Ralph, additional

Published
2021
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37. Maternal obesity programs white and brown adipose tissue transcriptome and lipidome in offspring in a sex-dependent manner

Author

Savva, Christina, primary, Helguero, Luisa A., additional, González-Granillo, Marcela, additional, Melo, Tânia, additional, Couto, Daniela, additional, Buyandelger, Byambajav, additional, Gustafsson, Sonja, additional, Liu, Jianping, additional, Domingues, Maria Rosário, additional, Li, Xidan, additional, and Korach-André, Marion, additional

Published
2021
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38. Valuing Bioactive Lipids from Green, Red and Brown Macroalgae from Aquaculture, to Foster Functionality and Biotechnological Applications

Author

Lopes, Diana, primary, Melo, Tânia, additional, Rey, Felisa, additional, Meneses, Joana, additional, Monteiro, Fátima Liliana, additional, Helguero, Luisa A., additional, Abreu, Maria Helena, additional, Lillebø, Ana Isabel, additional, Calado, Ricardo, additional, and Domingues, Maria Rosário, additional

Published
2020
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40. Lipidomic analysis of human primary hepatocytes following LXR activation with GW3965 identifies AGXT2L1 as a main target associated to changes in phosphatidylethanolamine

Author

Santinha, Deolinda, primary, Klopot, Anna, additional, Marques, Igor, additional, Ellis, Ewa, additional, Jorns, Carl, additional, Johansson, Helene, additional, Melo, Tânia, additional, Antonson, Per, additional, Jakobsson, Tomas, additional, Félix, Vítor, additional, Gustafsson, Jan-Åke, additional, Domingues, Maria Rosário, additional, Mode, Agneta, additional, and Helguero, Luisa A., additional

Published
2020
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42. Errors in protein synthesis increase the level of saturated fatty acids and affect the overall lipid profiles of yeast

Author

Araújo, Ana Rita D., primary, Melo, Tânia, additional, Maciel, Elisabete A., additional, Pereira, Clara, additional, Morais, Catarina M., additional, Santinha, Deolinda R., additional, Tavares, Joana F., additional, Oliveira, Helena, additional, Jurado, Amália S., additional, Costa, Vítor, additional, Domingues, Pedro, additional, Domingues, Maria Rosário M., additional, and Santos, Manuel A. S., additional

Published
2018
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44. Glycation impairs hepatic lipid metabolism and glucose tolerance in high-fat diet-induced obese rats, contributing to the onset of NAFLD

Author

Neves, Christian, Sereno, José, Simões, Cláudia, Rodrigues, Tiago, Castelhano, João, Gonçalves, Judith, Bento, Guida, Gonçalves, Sónia, Domingues, Maria Rosário, Seiça, Raquel, Castelo-Branco, Miguel, and Matafome, Paulo

Abstract

Objectives: Non-alcoholic fatty liver disease (NAFLD) is an insulin resistance- and lipotoxicity-related condition. We evaluated the role of glycation, often present without overt hyperglycaemia like in prediabetes and obesity1, in the dysregulation of hepatic lipid metabolism and in the induction of glucose dysmetabolism. Methods: An animal model of high-triglyceride diet-induced obesity (HFD), methylglyoxal (MG)-induced glycation (MG) or both (HFDMG) was used to asses liver lipid species in vivo by 1H nuclear magnetic resonance spectroscopy (MRS) and ex vivo by mass spectrometry (MS) and gas chromatography (GC), followed by western blotting and histologic analysis (n=6/group). Results: Although the high-fat diet increased liver fat mass in the MRS, only MG supplementation decreased unsaturated lipids, esterification and triglyceride levels (MRS and MS). HFDMG group also had decreased levels of plasmalogens (antioxidant phospholipids) and cardiolipins (mitochondrial phospholipids) in MS and GC analysis. Moreover, when combined to the high-fat diet glycation decreased insulin signalling and upregulated de novo fatty acid synthesis pathways. Conclusions: Although the high-fat diet alone increased liver fat fraction, only the association with glycation changed lipid metabolism, contributing to the hepatic lipotoxicity observed in NAFLD. Such mechanisms contribute to the vicious circle between insulin resistance, glucose dysmetabolism and NAFLD progression., Journal of International Society of Antioxidants in Nutrition & Health, Vol 3, No 2 (2016)

Published
2016
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