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Made available in DSpace on 2013-08-12T19:03:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-01 Made available in DSpace on 2013-09-30T18:19:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:33:15Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:33:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FUNDAMENTO: A radiografia de tórax e o eletrocardiograma (ECG) tem sido criticados em razão de sua baixa sensibilidade no diagnóstico da Hipertrofia Ventricular Esquerda (HVE) quando comparada ao ecocardiograma. Entretanto, esse exame não está disponível nos centros de atendimento primário para toda a população de hipertensos. OBJETIVO: Avaliar se a radiografia de tórax associada ao eletrocardiograma apresenta acurácia que justifique seu uso na detecção de HVE em hipertensos, bem como a utilidade do índice cardiotorácico e das radiografias oblíquas na avaliação das dimensões das câmaras cardíacas esquerdas. MÉTODOS: Estudo prospectivo realizado em 177 pacientes hipertensos consecutivos, através da radiografia do tórax, eletrocardiograma e ecocardiograma. Testes de acurácia foram utilizados para comparar esses métodos utilizando a ecocardiografia convencional como padrão de referência. RESULTADOS: O índice cardiotorácico mostrou sensibilidade de 17% para o diagnóstico de HVE, somente detectando alterações cardíacas mais acentuadas. As incidências póstero-anterior e perfil tiveram sensibilidade de 52%, aumentando para 54% quando a radiografia de tórax foi associada ao eletrocardiograma. As incidências oblíquas não melhoraram significativamente a acurácia da radiografia de tórax que, por sua vez, apresentou alta especificidade e boa sensibilidade para a detecção do aumento da aorta. Foi interessante notar que essa alteração estava presente em metade dos hipertensos com HVE. CONCLUSÃO: A associação da radiografia de tórax com eletrocardiograma é útil na avaliação inicial de hipertensos para o diagnóstico de hipertrofia ventricular esquerda, especialmente se a ecocardiografia não estiver disponível. Background: Chest radiography and electrocardiogram have been criticized due to their low sensitivity for Left Ventricular Hypertrophy diagnosis compared to echocardiogram. This one, however, is not available in primary health care centers to all hypertensive population.Objective: To evaluate whether the association chest radiography-electrocardiogram provides the accuracy to justify its use in left ventricular hypertrophy detection in hypertensive patients, as well as the usefulness of the cardiothoracic ratio and oblique radiographs in relation to frontal and lateral views in evaluating dimensions of left cardiac chambers.Methods: This was a prospective study including 177 consecutive hypertensive patients through chest radiography, electrocardiogram and echocardiography. Accuracy test was used to compare these methods using echocardiography as gold standard.Results: The cardiothoracic ratio showed 17% sensitivity for detection of left ventricular hypertrophy, only indicating cardiac alterations at an advanced stage. Frontal plus lateral views showed sensitivity of 52%, which rose to 54% when chest radiography was associated with electrocardiogram. The oblique views did not significantly improve chest radiography accuracy. Chest radiography presented high specificity and elevated sensitivity for detection of aortal enlargement. Interestingly, this alteration was present in half of the hypertensive patients with left ventricular hypertrophy.Conclusion: We conclude that the association chest radiography-electrocardiogram is useful for the screening of hypertensive patients for the diagnosis of left ventricular hypertrophy, especially if echocardiogram is unavailable. (Arq Bras Cardiol 2012;99(3):825-833) Univ Estadual Paulista, UNESP, Fac Med Botucatu, Dept Doencas Tropicais & Diagnost Imagem, Botucatu, SP, Brazil Univ Estadual Paulista, UNESP, Fac Med Botucatu, Dept Clin Med, Botucatu, SP, Brazil Univ Estadual Paulista, UNESP, Fac Med Botucatu, Dept Doencas Tropicais & Diagnost Imagem, Botucatu, SP, Brazil Univ Estadual Paulista, UNESP, Fac Med Botucatu, Dept Clin Med, Botucatu, SP, Brazil FAPESP: 04/09460-5

Made available in DSpace on 2022-04-28T20:51:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-05-29 Hepatitis C is transmitted primarily parenterally by contaminated blood and is often associated with: intravenous drug abuse, invasive procedures, blood transfusions, acupuncture, tattooing, and alcohol and tobacco use. This study aimed to quantify and evaluate the risk factors among blood donors, volunteer blood donors and replacement individuals, infected or not by the C virus. The main transmission routes of C virus were identified in 55 men and 25 women (GI) monitored by the Ambulatory Unit of the Department of Tropical Diseases, Botucatu Medical School, and in 24 men and 26 women (GII), all active blood donors at the Bauru State Hospital Transfusional Agency. Both groups were similar in: tobacco and alcohol consumption, sexual behavior, tattooing and illicit drug use. The duration of alcohol and tobacco consumption and blood transfusions in GI were longer, whereas the option for steady partners, condom use, disposable materials and piercings were predominant in GII. In conclusion, the risk factors for hepatitis C demonstrate the necessity of health policies that act on the primary and secondary prevention levels (respectively, reduction of infection incidence and hepatopathy risk). Bauru State Hospital Botucatu Medical School São Paulo State University, UNESP, Bauru, São Paulo State Department of Tropical Diseases Botucatu Medical School São Paulo State University, UNESP, Botucatu, São Paulo State Assessoria de Planejamento do Hospital Estadual Bauru Universidade Estadual Paulista, UNESP, Av. Eng. Luis Edmundo Carrijo Coube, 1-100, Bauru, SP, 17.033-360 Bauru State Hospital Botucatu Medical School São Paulo State University, UNESP, Bauru, São Paulo State Department of Tropical Diseases Botucatu Medical School São Paulo State University, UNESP, Botucatu, São Paulo State Assessoria de Planejamento do Hospital Estadual Bauru Universidade Estadual Paulista, UNESP, Av. Eng. Luis Edmundo Carrijo Coube, 1-100, Bauru, SP, 17.033-360

Made available in DSpace on 2013-08-12T18:00:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 Made available in DSpace on 2013-09-30T18:38:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:27Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:34:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G(1) = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G(2) = 27); and patients on HAART with undetectable VL for at least the past six months (G(3) = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G(4) = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-alpha, IL-2, INF-gamma, IL-4 and IL-10. All patients were submitted to VL determination and CD4(+) and CD8(+) T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (>80% - r(2) > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-gamma required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-gamma in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4(+) T cells, mainly during primary infection, persisting only in those with INF-gamma phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur. São Paulo State Univ, UNESP, Botucatu Med Sch, Dept Trop Dis, Botucatu, SP, Brazil São Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil São Paulo State Univ, UNESP, Botucatu Med Sch, Dept Trop Dis, Botucatu, SP, Brazil São Paulo State Univ, UNESP, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil

Made available in DSpace on 2022-04-28T20:48:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 The unfavorable evolution of a young ovine during hyperimmunization process with Crotalus durissus terrificus venom was investigated in order to differentiate its origin between ophidic envenomation and copper toxicosis. Clinical, laboratory, necroscopic and histological exams as well as evaluation and measurement of heavy metals (copper) in the kidneys and in the liver were carried out. Blood counts revealed anemia and serological tests showed high levels of blood urea nitrogen, creatinine, aspartate aminotransferase, creatine phosphokinase, total bilirubin and indirect bilirubin; which indicates liver, kidney and skeletal muscle damages. At necropsy, the animal presented hepatopathy and nephropathy. Histological examination revealed renal and hepatic features that may imply copper intoxication. Copper levels were 237.8 μg/g in the liver and 51.2 μg/g in the kidneys. Although the amount of metal found in both organs was below the level that can cause death, according to the literature, anatomopathological signs were suggestive of copper intoxication. Therefore, the hypothesis of metal toxicosis during the hyperimmunization process became more consistent than the crotalic envenomation one. Department of Tropical Diseases Botucatu Medical School São Paulo State University, Botucatu, São Paulo State Center for the Study of Venoms and Venomous Animals CEVAP São Paulo State University, Botucatu, São Paulo State Radiobiology Supervision Nuclear Energy Research Institute IPEN/CNEN-SP, São Paulo, São Paulo State Clinical Laboratory of Veterinary Veterinary Medicine and Animal Husbandry School São Paulo State University, Botucatu, São Paulo State Centro de Estudos de Venenos e Animais Peçonhentos CEVAP UNESP, Caixa Postal 577, Botucatu, SP, 18618-000, Brasil Department of Tropical Diseases Botucatu Medical School São Paulo State University, Botucatu, São Paulo State Center for the Study of Venoms and Venomous Animals CEVAP São Paulo State University, Botucatu, São Paulo State Clinical Laboratory of Veterinary Veterinary Medicine and Animal Husbandry School São Paulo State University, Botucatu, São Paulo State Centro de Estudos de Venenos e Animais Peçonhentos CEVAP UNESP, Caixa Postal 577, Botucatu, SP, 18618-000, Brasil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:43:54Z No. of bitstreams: 1 S1806-37132006000500011.pdf: 62774 bytes, checksum: 6456d5f897795b22473450658432ca68 (MD5) Made available in DSpace on 2013-08-22T18:43:54Z (GMT). No. of bitstreams: 1 S1806-37132006000500011.pdf: 62774 bytes, checksum: 6456d5f897795b22473450658432ca68 (MD5) Previous issue date: 2006-10-01 Made available in DSpace on 2013-09-30T18:19:25Z (GMT). No. of bitstreams: 2 S1806-37132006000500011.pdf: 62774 bytes, checksum: 6456d5f897795b22473450658432ca68 (MD5) S1806-37132006000500011.pdf.txt: 26036 bytes, checksum: cc57cd19ccb6a9ae5553ff4dd04d7c3c (MD5) Previous issue date: 2006-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:38Z No. of bitstreams: 2 S1806-37132006000500011.pdf: 62774 bytes, checksum: 6456d5f897795b22473450658432ca68 (MD5) S1806-37132006000500011.pdf.txt: 26036 bytes, checksum: cc57cd19ccb6a9ae5553ff4dd04d7c3c (MD5) Made available in DSpace on 2014-05-20T13:34:38Z (GMT). No. of bitstreams: 2 S1806-37132006000500011.pdf: 62774 bytes, checksum: 6456d5f897795b22473450658432ca68 (MD5) S1806-37132006000500011.pdf.txt: 26036 bytes, checksum: cc57cd19ccb6a9ae5553ff4dd04d7c3c (MD5) Previous issue date: 2006-10-01 OBJETIVO: Avaliar os resultados do teste tuberculínico e relacioná-los com a presença ou não de tuberculose em atividade e com a contagem de linfócitos T CD4+/CD8+. MÉTODOS: Foram revisados 802 prontuários de pacientes com síndrome da imunodeficiência adquirida atendidos no período de agosto de 1985 a março de 2003. Cento e oitenta e cinco pacientes realizaram o teste tuberculínico (23,1%) e, destes, 107 eram do sexo masculino (57,8%). A média de idade no grupo de reatores ao teste tuberculínico foi de 30,6 anos, com desvio-padrão de 6,62 anos, e entre os não reatores de 34,45 anos com desvio-padrão de 10,32 anos. Foram constituídos dois grupos de estudo: reatores ao teste tuberculínico, com 28 pacientes, e não reatores ao teste tuberculínico, com 157 pacientes. RESULTADOS: Grande parte dos indivíduos foi pouco responsiva ao teste tuberculínico. Constatou-se, no grupo de reatores, maior porcentagem de indivíduos com tuberculose ativa à época da realização do teste, quando se comparou com os não reatores. Dez pacientes entre os reatores e onze entre os não reatores apresentavam alguma forma clínica de tuberculose em atividade à época da realização do teste, sendo que seis do primeiro grupo e oito do segundo tinham contagem de linfócitos T CD4+ menor que 200 células/mm³. CONCLUSÃO: Indurações maiores do que 5 mm não se relacionaram com contagens absolutas mais altas de células T CD4+. OBJECTIVE: To evaluate tuberculin test results and relate them to the presence or absence of active tuberculosis, as well as to CD4+ and CD8+ T-lymphocyte counts. METHOD: The charts of 802 patients with acquired immunodeficiency syndrome treated between August of 1985 and March of 2003 were reviewed. of the 185 patients submitted to tuberculin tests (23.1%), 107 (57.8%) were male, and 78 (42.2%) were female. Patients were divided into two study groups: tuberculin test reactors (n = 28); and tuberculin test non-reactors (n = 157). Among the reactors, the mean age was 30.60 years, with a standard deviation of 6.62 years, compared with 34.45 years, with a standard deviation of 10.32 years, among the non-reactors. RESULTS: Most of the individuals tested presented only a mild response to the tuberculin test. We found that, at the time of the test, the percentage of individuals with active tuberculosis was greater in the reactor group than in the non-reactor group. During the test period, 10 reactor group patients and 11 non-reactor group patients presented some clinical form of active tuberculosis. In addition, CD4+ T-lymphocyte counts were lower than 200 cells/mm³ in 6 reactor group patients and in 8 non-reactor group patients. CONCLUSION: Indurations greater than 5 mm were unrelated to higher absolute CD4+ T-cell counts. Universidade Estadual Paulista 'Julio Mesquista Filho' Faculdade de Medicina de Botucatu Universidade Federal do Ceará (UFC) Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem Universidade Estadual Paulista 'Julio Mesquista Filho' Faculdade de Medicina de Botucatu Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem

To report nine additional well-defined cases with infiltrative myelopathy by paracoccidioidomycosis (PCM), to describe the specific lesions and infection-related stromal abnormalities, to review the literature on this type of involvement and to introduce a new cause of granulomatous lesions of bone marrow.Different bone marrow specimens were studied (aspirated smears, aspirated clots, biopsy imprints and biopsies) from nine patients with acute or subacute forms of PCM known to have PCM infiltrative myelopathy.The biopsy specimens were the best for demonstrating bone marrow involvement by PCM. The lesions varied from compact and focal granulomas with few fungal cells to numerous disseminated fungal cells within a loose granulomatous inflammatory reaction, with a continuum between these extremes suggesting a spectrum of immune response to the fungi. Other findings such as bone marrow fibrosis, parenchymal coagulative necrosis and bone necrosis were also observed in the affected areas.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:22:02Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:40:13Z : No. of bitstreams: 1 2-s2.0-33846294386.pdf: 286370 bytes, checksum: 7fdca13ec6a50f7e9f657a080ff0e3d0 (MD5) Made available in DSpace on 2014-05-27T11:22:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-11-01 Laboratory profile of young ovines was studied in order to evaluate and compare their antiserum production from natural and Cobalt-60 irradiated Crotalus durissus terrificus (C.d.t.) venoms. The parameters analyzed included complete blood count, and urea, creatinine, aspartate aminotransferase, total proteins, albumin and globulin serum measurements. Three groups of six animals each were used. Group 1 (G1) received natural C.d.t. venom; Group 2 (G2) received irradiated C.d.t. venom; and Group 3 (G3) was used as control and did not receive venom, only adjuvants, using seven venom inoculations. During the experimental period, animals were fortnightly weighed. According to clinical and weight evaluation, sheep in post-weaning phase showed no changes in their physiological profiles but had excellent weight gain. The parameters analyzed were not statistically different (p

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:06:20Z No. of bitstreams: 1 S0037-86822004000100008.pdf: 129254 bytes, checksum: 7e09e9e460e766045d0c9412a70b5ae0 (MD5) Made available in DSpace on 2013-08-22T19:06:20Z (GMT). No. of bitstreams: 1 S0037-86822004000100008.pdf: 129254 bytes, checksum: 7e09e9e460e766045d0c9412a70b5ae0 (MD5) Previous issue date: 2004-02-01 Made available in DSpace on 2013-09-30T18:23:41Z (GMT). No. of bitstreams: 2 S0037-86822004000100008.pdf: 129254 bytes, checksum: 7e09e9e460e766045d0c9412a70b5ae0 (MD5) S0037-86822004000100008.pdf.txt: 24506 bytes, checksum: aeee34546544e1a7fb9855f346263f5e (MD5) Previous issue date: 2004-02-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:24Z No. of bitstreams: 2 S0037-86822004000100008.pdf: 129254 bytes, checksum: 7e09e9e460e766045d0c9412a70b5ae0 (MD5) S0037-86822004000100008.pdf.txt: 24506 bytes, checksum: aeee34546544e1a7fb9855f346263f5e (MD5) Made available in DSpace on 2014-05-20T13:34:24Z (GMT). No. of bitstreams: 2 S0037-86822004000100008.pdf: 129254 bytes, checksum: 7e09e9e460e766045d0c9412a70b5ae0 (MD5) S0037-86822004000100008.pdf.txt: 24506 bytes, checksum: aeee34546544e1a7fb9855f346263f5e (MD5) Previous issue date: 2004-02-01 Com o objetivo de estudar a soroprevalência de vírus linfotrópico de células T humanas I/II (HTLV-I/II), vírus da imunodeficiência humana, sífilis e toxoplasmose, em gestantes atendidas em unidade básicas de saúde do município de Botucatu - São Paulo - Brasil, bem como os fatores de risco para a infecção pelo HTLV -I/II, foram realizados inquérito sorológico e avaliação dos resultados de exames solicitados na rotina do prénatal. em 913 gestantes, a soroprevalência de HTLV- I e de HTLV- II foi de 0,1%. Sífilis, toxoplasmose e infecção pelo HIV foram encontradas. Nenhum dos fatores de risco pesquisados mostrou-se seguro para identificar gestantes com infecção pelo HTLV- I/II. A comparação da proporção de gestantes infectadas e de doadores de sangue da região sudeste do Brasil com testes reagentes para HTLV- I/II não mostrou diferença estatística. In order to asses the soroprevalence of HTLV- I/II, HIV, siphylis, and toxoplasmosis infection among pregnant women seen at Basic Health Units in the municipality of Botucatu - São Paulo - Brazil, and risk factors for HTLV- I/II infection, sorologic surveys were carried out and results of sorologic surveys carried in pregnance routine evaluation were assessed. Seroprevalence was 0.1% (1 in 913) for both HTLV-I and HTLV-II. Seroprevalence was 0.1% (1 in 913) for both HTLV-I and HTLV-II. Siphylis, toxoplasmosis and HIV were identified. None of the factors or characteristics risks was found to be useful to identify those prenant women HTLV-II infected. Infection among pregnant women and southeast brazilian blood donnors were not diferent in statistical evaluation. Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Pediatria Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Pediatria Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:07:20Z No. of bitstreams: 1 S0037-86822013000100088.pdf: 799341 bytes, checksum: fdee9a6aaa5088745bb2556126de8289 (MD5) Made available in DSpace on 2013-08-22T19:07:20Z (GMT). No. of bitstreams: 1 S0037-86822013000100088.pdf: 799341 bytes, checksum: fdee9a6aaa5088745bb2556126de8289 (MD5) Previous issue date: 2013-01-01 Made available in DSpace on 2013-09-30T20:11:46Z (GMT). No. of bitstreams: 2 S0037-86822013000100088.pdf: 799341 bytes, checksum: fdee9a6aaa5088745bb2556126de8289 (MD5) S0037-86822013000100088.pdf.txt: 18528 bytes, checksum: 5d2abb4b49ce36b9754cf15aacf2c65a (MD5) Previous issue date: 2013-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:18:14Z No. of bitstreams: 2 S0037-86822013000100088.pdf: 799341 bytes, checksum: fdee9a6aaa5088745bb2556126de8289 (MD5) S0037-86822013000100088.pdf.txt: 18528 bytes, checksum: 5d2abb4b49ce36b9754cf15aacf2c65a (MD5) Made available in DSpace on 2014-05-20T15:18:14Z (GMT). No. of bitstreams: 2 S0037-86822013000100088.pdf: 799341 bytes, checksum: fdee9a6aaa5088745bb2556126de8289 (MD5) S0037-86822013000100088.pdf.txt: 18528 bytes, checksum: 5d2abb4b49ce36b9754cf15aacf2c65a (MD5) Previous issue date: 2013-01-01 ,,,,,INTRODUCTION:,,,,, While the incidence of HIV infection and AIDS is increasing in small Brazilian cities, epidemiological studies are often conducted in large urban centers. ,,,,,,,,,,,,,METHODS: ,,,,, Our group conducted a retrospective analysis of survival determinants among 358 patients who attended a reference unit in a small city.,,,,,,,,,,,,,,,,,,RESULTS: ,,,,,Death risk was lower among men that had sex with men, patients with an HIV-seropositive partner, and those admitted after highly active antiretroviral therapy (HAART) was available. ,,,,,,,,,,,,,CONCLUSIONS:,,,,, The study documents the striking beneficial effect of HAART. The finding of other groups with improved survival may aid in the development of programmatic strategies.,,,, Universidade Federal de Mato Grosso do Sul Departamento de Ciências Biológicas e Saúde Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem

Chest radiography and electrocardiogram have been criticized due to their low sensitivity for Left Ventricular Hypertrophy diagnosis compared to echocardiogram. This one, however, is not available in primary health care centers to all hypertensive population.To evaluate whether the association chest radiography-electrocardiogram provides the accuracy to justify its use in left ventricular hypertrophy detection in hypertensive patients, as well as the usefulness of the cardiothoracic ratio and oblique radiographs in relation to frontal and lateral views in evaluating dimensions of left cardiac chambers.This was a prospective study including 177 consecutive hypertensive patients through chest radiography, electrocardiogram and echocardiography. Accuracy test was used to compare these methods using echocardiography as gold standard.The cardiothoracic ratio showed 17% sensitivity for detection of left ventricular hypertrophy, only indicating cardiac alterations at an advanced stage. Frontal plus lateral views showed sensitivity of 52%, which rose to 54% when chest radiography was associated with electrocardiogram. The oblique views did not significantly improve chest radiography accuracy. Chest radiography presented high specificity and elevated sensitivity for detection of aortal enlargement. Interestingly, this alteration was present in half of the hypertensive patients with left ventricular hypertrophy.We conclude that the association chest radiography-electrocardiogram is useful for the screening of hypertensive patients for the diagnosis of left ventricular hypertrophy, especially if echocardiogram is unavailable.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:43:32Z No. of bitstreams: 1 S1678-91992010000100003.pdf: 22083 bytes, checksum: 0187d0c8fcfaabfc0cec136e2063effa (MD5) Made available in DSpace on 2013-08-22T18:43:32Z (GMT). No. of bitstreams: 1 S1678-91992010000100003.pdf: 22083 bytes, checksum: 0187d0c8fcfaabfc0cec136e2063effa (MD5) Previous issue date: 2010-01-01 Made available in DSpace on 2013-09-30T18:17:52Z (GMT). No. of bitstreams: 2 S1678-91992010000100003.pdf: 22083 bytes, checksum: 0187d0c8fcfaabfc0cec136e2063effa (MD5) S1678-91992010000100003.pdf.txt: 6185 bytes, checksum: 32c07ca259ab2240a75717f717f5a986 (MD5) Previous issue date: 2010-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:33:43Z No. of bitstreams: 2 S1678-91992010000100003.pdf: 22083 bytes, checksum: 0187d0c8fcfaabfc0cec136e2063effa (MD5) S1678-91992010000100003.pdf.txt: 6185 bytes, checksum: 32c07ca259ab2240a75717f717f5a986 (MD5) Made available in DSpace on 2014-05-20T13:33:43Z (GMT). No. of bitstreams: 2 S1678-91992010000100003.pdf: 22083 bytes, checksum: 0187d0c8fcfaabfc0cec136e2063effa (MD5) S1678-91992010000100003.pdf.txt: 6185 bytes, checksum: 32c07ca259ab2240a75717f717f5a986 (MD5) Previous issue date: 2010-01-01 UNESP Emeritus of the Botucatu Medical School FAMESP Medical and Hospital Development Foundation UNESP Emeritus of the Botucatu Medical School

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:24:33Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:41:13Z : No. of bitstreams: 1 2-s2.0-78149421826.pdf: 1381462 bytes, checksum: de9ad9d0b3960645486c36dca048dc3c (MD5) Made available in DSpace on 2014-05-27T11:24:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-12-01 The ELISA technique was used to evaluate and compare young ovine humoral immune response during crotalic antiserum production. Animals were clinically evaluated throughout this process, and the neutralizing capacity of antisera raised against natural (NV) and Cobalt-60 irradiated (IrV) venoms of Crotalus durissus terrificus (C.d.t.) was verified by means of in vitro challenges. Three groups of six animals each were used: G1 received NV; G2 was inoculated with IrV; and G3 was used as control. Animals received six immunizations during 84 days at 14-day intervals. ELISA of antibody profile showed significant difference (p

Made available in DSpace on 2013-09-27T14:54:10Z (GMT). No. of bitstreams: 1 WOS000264071400011.pdf: 453007 bytes, checksum: 1a30bebaf25f96f84063290d1ff2e778 (MD5) Previous issue date: 2009-03-01 Made available in DSpace on 2013-09-30T18:18:57Z (GMT). No. of bitstreams: 1 WOS000264071400011.pdf: 453007 bytes, checksum: 1a30bebaf25f96f84063290d1ff2e778 (MD5) Previous issue date: 2009-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:19Z No. of bitstreams: 1 WOS000264071400011.pdf: 453007 bytes, checksum: 1a30bebaf25f96f84063290d1ff2e778 (MD5) Made available in DSpace on 2014-05-20T13:34:19Z (GMT). No. of bitstreams: 1 WOS000264071400011.pdf: 453007 bytes, checksum: 1a30bebaf25f96f84063290d1ff2e778 (MD5) Previous issue date: 2009-03-01 To report the first eight bone marrow necrosis (BMN) cases related to paracoccidioidomycosis (PCM) from patient autopsies with well-documented bone marrow (BM) histology and cytology.A retrospective evaluation was performed on BM specimens from eight autopsied patients from Botucatu University Hospital with PCM-related BMN. Relevant BMN literature was searched and analysed.All eight patients had acute PCM. Six had histological only (biopsies) and two cytological only (smears) specimens. Five biopsy specimens revealed severe and one mild coagulation patterned necrotic areas. Five had osteonecrosis. The cytological specimens also showed typical BMN patterns. Paracoccidioides brasiliensis yeast forms were visible within necrotic areas in all cases. São Paulo State Univ, UNESP, Botucatu Med Sch, Clin Dept,Haematol Serv, BR-18618970 Botucatu, SP, Brazil São Paulo State Univ, Dept Trop Dis & Image Diag, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil São Paulo State Univ, Dept Pathol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil São Paulo State Univ, Dept Dermatol & Radiotherapy, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil São Paulo State Univ, UNESP, Botucatu Med Sch, Clin Dept,Haematol Serv, BR-18618970 Botucatu, SP, Brazil São Paulo State Univ, Dept Trop Dis & Image Diag, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil São Paulo State Univ, Dept Pathol, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil São Paulo State Univ, Dept Dermatol & Radiotherapy, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:06:37Z No. of bitstreams: 1 S0037-86822007000500005.pdf: 163435 bytes, checksum: c2b72100310e22589016d96ea2d5d1a5 (MD5) Made available in DSpace on 2013-08-22T19:06:37Z (GMT). No. of bitstreams: 1 S0037-86822007000500005.pdf: 163435 bytes, checksum: c2b72100310e22589016d96ea2d5d1a5 (MD5) Previous issue date: 2007-10-01 Made available in DSpace on 2013-09-30T18:19:50Z (GMT). No. of bitstreams: 2 S0037-86822007000500005.pdf: 163435 bytes, checksum: c2b72100310e22589016d96ea2d5d1a5 (MD5) S0037-86822007000500005.pdf.txt: 29190 bytes, checksum: b7ced3b9fc0f44b60cc1c6fed6321f02 (MD5) Previous issue date: 2007-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:34:24Z No. of bitstreams: 2 S0037-86822007000500005.pdf: 163435 bytes, checksum: c2b72100310e22589016d96ea2d5d1a5 (MD5) S0037-86822007000500005.pdf.txt: 29190 bytes, checksum: b7ced3b9fc0f44b60cc1c6fed6321f02 (MD5) Made available in DSpace on 2014-05-20T13:34:24Z (GMT). No. of bitstreams: 2 S0037-86822007000500005.pdf: 163435 bytes, checksum: c2b72100310e22589016d96ea2d5d1a5 (MD5) S0037-86822007000500005.pdf.txt: 29190 bytes, checksum: b7ced3b9fc0f44b60cc1c6fed6321f02 (MD5) Previous issue date: 2007-10-01 O objetivo deste estudo foi o de procurar evidências da transmissão vetorial da doença de Chagas, nos domicílios e peridomicílios de indivíduos residentes em municípios da região de Botucatu, que tiveram xenodiagnóstico positivos. Foram estudados 58 indivíduos e foi coletada amostra do sangue para a realização de exames laboratoriais. Os resultados deste estudo mostraram que os indivíduos, de ambos os grupos, tinham baixa escolaridade e exerciam profissões que não exigiam qualificações técnicas. Houve discreto predomínio de indivíduos do sexo feminino. Quando comparadas às condições anteriores, verificou-se que houve discreta melhora nas condições de habitação, por outro lado, houve aumento de moradores em zona rural. Os indivíduos nascidos antes de 1983, apresentaram conhecimento e contato com triatomídeo estaticamente mais elevado quando comparado com os nascidos a partir 1983. A análise e comparação dos resultados das sorologias, referentes aos hemaglutinação passiva indireta, imunofluorescência indireta e ensaio imunoenzimático, mostrou que o ELISA apresentou maior sensibilidade. Os resultados deste estudo mostram que a população nascida a partir de 1983 não conhecia o vetor transmissor da doença de Chagas. The aim of this study was to look for evidence of vector transmission of Chagas disease, in and around the homes of individuals living in municipalities in the Botucatu region who presented a positive xenodiagnosis. Fifty-eight subjects were studied and blood samples were collected from them for laboratory analysis. The results from this study showed that the individuals in both groups presented low formal education levels and had been working in unskilled professions There was a slight predominance of females. In comparison with previous conditions, it was observed that there had been a slight improvement in the subjects' housing conditions, but on the other hand there had been an increase in the numbers of individuals living in rural areas. Subjects born before 1983 presented statistically greater knowledge of and contact with triatomines than did those born from 1983 onwards. Analysis and comparison of the results from the serological tests, including indirect passive hemagglutination, indirect immunofluorescence and immunoenzymatic assay, showed that ELISA presented greatest sensitivity. The results from this study have shown that the population born from 1983 onwards did not know about the transmission vector for Chagas disease. Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Enfermagem Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Enfermagem Universidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Doenças Tropicais e Diagnóstico por Imagem

The aim of this study was to look for evidence of vector transmission of Chagas disease, in and around the homes of individuals living in municipalities in the Botucatu region who presented a positive xenodiagnosis. Fifty-eight subjects were studied and blood samples were collected from them for laboratory analysis. The results from this study showed that the individuals in both groups presented low formal education levels and had been working in unskilled professions There was a slight predominance of females. In comparison with previous conditions, it was observed that there had been a slight improvement in the subjects' housing conditions, but on the other hand there had been an increase in the numbers of individuals living in rural areas. Subjects born before 1983 presented statistically greater knowledge of and contact with triatomines than did those born from 1983 onwards. Analysis and comparison of the results from the serological tests, including indirect passive hemagglutination, indirect immunofluorescence and immunoenzymatic assay, showed that ELISA presented greatest sensitivity. The results from this study have shown that the population born from 1983 onwards did not know about the transmission vector for Chagas disease.

Chronic viral hepatitis is currently the most common indication for liver transplantation (OLT). Knowing the serological profile of patients on the liver transplant waiting list (LTWL) is essential to manage prophylactic and therapeutic strategies pre- and post-OLT. The aim of this study was to determine the hepatitis B virus (HBV) and hepatitis C virus (HCV) serological profile on the LTWL. Methods. Serological data were collected from 44 candidates included on the LTWL from May 2003 to November 2004. HBV and HCV serological profiles were performed by microenzyme immunoassay. Results. Twenty-eight patients (66.7%) lacked HBV serological markers. Anti-HBs was detected in 9.5% and was positive for HBsAg, anti-HBc, IgM anti-HBc, or HbeAg in 4.8% of patients, probably related to reactivation of chronic infection. In 7.1% of patients, the markers demonstrated serological cure of infection. In HCV patients, 41.5% were positive. There was HBV and HCV co-infection in 12.2% of patients. Conclusion. HBV infection in 21.4% of the patients corroborates the need to use more efficient protocols for prophylactic and therapeutic management pre- and post-OLT. The high prevalence of HCV infection reinforces the need to follow adequate protocols to avoid related complications and guarantee rational and universal use of more efficient drugs.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:21:51Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:34:43Z : No. of bitstreams: 1 2-s2.0-33645884428.pdf: 245365 bytes, checksum: bfea6279fe6b6ca984fc5d81ce119a7b (MD5) Made available in DSpace on 2014-05-27T11:21:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-04-26 The aim of this paper was to evaluate the immune reconstitution of HIV-1 patients subjected to highly active antiretroviral therapy (HAART) for two years or more according to CD 45RA and CD 45RO cell count; determination of IL-2, IFN-γ, IL-4, IL-10 and TNF-α serum levels; CD 4 + T and CD 8 + T lymphocyte count; and plasma viral load (VL) determination. For this purpose, a cross sectional study was carried out in the Tropical Diseases Area, Botucatu School of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil. Between June 2001 and April 2002, 37 HIV-1 infected patients were evaluated, 13 with treatment indication but untreated (G1), 9 subjected to HAART for 5-7 months (G2), and 15 treated for two years or more (G3); both treated groups used medication regularly and without failure. Forty-nine normal individuals were studied as controls (GC-1 and GC-2). There was a tendency (p[G2=G3]; pG3; G2=intermediate]>GC-1; p[GC-2=G3]; pGC-1; p

Made available in DSpace on 2021-07-14T10:39:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-12. Added 1 bitstream(s) on 2021-07-14T11:34:57Z : No. of bitstreams: 1 S1678-91992005000400006.pdf: 363142 bytes, checksum: c01bd4adc7ea040098d4aa1216611db0 (MD5) ELISA was used to evaluate, accompany, and compare the humoral immune response of Swiss mice during hyperimmunization with native and Cobalt-60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge, and the side effects were assessed. The animals immunization with one LD50 of each venom occurred on days 1, 15, 21, 30, and 45, when blood samples were collected; challenges happened on the 60th day. Results showed that ELISA was efficient in evaluating, accompanying and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co-irradiation. The sera produced with native venom showed neutralizing potency and capacity similar to those of the sera produced with irradiated venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom, however, necrosis and death occurred in animals inoculated with native venom. Universidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals Universidade Estadual Paulista, Botucatu School of Medicine IPEN/CNEN, Nuclear Energy Research Institute Universidade Estadual Paulista, School of Engineering Universidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals Universidade Estadual Paulista, Botucatu School of Medicine Universidade Estadual Paulista, School of Engineering

Made available in DSpace on 2021-07-14T10:30:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-12. Added 1 bitstream(s) on 2021-07-14T11:31:55Z : No. of bitstreams: 1 S1678-91992005000400009.pdf: 227196 bytes, checksum: 0558ae490fb1b3613f8725349788fa3e (MD5) Among domestic animals, dogs are considered to be the major reservoirs of trypanosomatids and, due to their proximity to man, the presence of these parasites in dogs is an alert to actions aiming at triatomine control. Fifty dogs (26 males and 24 females), aged from 2 months to 15 years, belonging to 30 chronic Chagas’ disease individuals from 15 different municipalities in the western region of São Paulo State, Brazil, were subjected to blood collection for the following tests: artificial xenodiagnosis, blood culture, and Polymerase Chain Reaction (PCR). Forty-three (86%) out of 50 dogs were positive to at least one of the tests performed; 34 (68%) were positive to xenodiagnosis, 30 (60%) to blood culture, and 25 (50%) to PCR for T. cruzi and/or T. rangeli. Although triatomines were not detected during the intra and peridomiciliary inspections in the dog owners’ residences, the results obtained demonstrate that there is a transmission cycle whereby triatomine vector may be participating in the infection epidemiological chain. Universidade Estadual Paulista, Botucatu School of Medicine Universidade Estadual Paulista, Institute of Biosciences Universidade Estadual do Paraná Universidade Estadual Paulista, Botucatu School of Veterinary Medicine and Animal Husbandry Universidade Estadual Paulista, Botucatu School of Medicine Universidade Estadual Paulista, Institute of Biosciences Universidade Estadual Paulista, Botucatu School of Veterinary Medicine and Animal Husbandry

Made available in DSpace on 2021-07-14T10:39:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-09. Added 1 bitstream(s) on 2021-07-14T11:34:56Z : No. of bitstreams: 1 S1678-91992005000300008.pdf: 630382 bytes, checksum: 79322498599e862b0d903c9e02291228 (MD5) ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom. CNEN, IPEN Universidade Estadual Paulista, School of Engineering Universidade Estadual Paulista, Botucatu School of Medicine Universidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals Universidade Estadual Paulista, School of Engineering Universidade Estadual Paulista, Botucatu School of Medicine Universidade Estadual Paulista, Center for the Study of Venoms and Venomous Animals