Search

Your search keyword '"Domingo, Gonzalo J."' showing total 361 results
Start Over Author "Domingo, Gonzalo J."
361 results on '"Domingo, Gonzalo J."'

3. Multiplex Human Malaria Array: Quantifying Antigens for Malaria Rapid Diagnostics

Author

Jang, Ihn Kyung, Tyler, Abby, Lyman, Chris, Rek, John C, Arinaitwe, Emmanuel, Adrama, Harriet, Murphy, Maxwell, Imwong, Mallika, Proux, Stephane, Haohankhunnatham, Warat, Barney, Rebecca, Rashid, Andrew, Kalnoky, Michael, Kahn, Maria, Golden, Allison, Nosten, François, Greenhouse, Bryan, Gamboa, Dionicia, and Domingo, Gonzalo J

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Clinical Sciences, Vector-Borne Diseases, Infectious Diseases, Malaria, Rare Diseases, Infection, Good Health and Well Being, Antigens, Protozoan, DNA, Protozoan, Diagnostic Tests, Routine, Humans, Multiplex Polymerase Chain Reaction, Plasmodium, Sensitivity and Specificity, Species Specificity, Medical and Health Sciences, Tropical Medicine, Biomedical and clinical sciences, Health sciences
Abstract

Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex™ Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs-Plasmodium falciparum-specific histidine-rich protein 2 (HRP2), P. falciparum-specific lactate dehydrogenase (Pf LDH), Plasmodium vivax -specific LDH (Pv LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction-confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. P. falciparum culture strains and samples from Peru indicated that HRP2 and Pf LDH combined improves detection of P. falciparum parasites with hrp2 and hrp3 deletions. This array can be used for antigen-based malaria screening and detecting hrp2/3 deletion mutants of P. falciparum.

Published
2020

4. Simultaneous Quantification of Plasmodium Antigens and Host Factor C-Reactive Protein in Asymptomatic Individuals with Confirmed Malaria by Use of a Novel Multiplex Immunoassay.

Author

Jang, Ihn Kyung, Tyler, Abby, Lyman, Chris, Kahn, Maria, Kalnoky, Michael, Rek, John C, Arinaitwe, Emmanuel, Adrama, Harriet, Murphy, Maxwell, Imwong, Mallika, Ling, Clare L, Proux, Stephane, Haohankhunnatham, Warat, Rist, Melissa, Seilie, Annette M, Hanron, Amelia, Daza, Glenda, Chang, Ming, Das, Smita, Barney, Rebecca, Rashid, Andrew, Landier, Jordi, Boyle, David S, Murphy, Sean C, McCarthy, James S, Nosten, François, Greenhouse, Bryan, and Domingo, Gonzalo J

Subjects
Humans, Plasmodium, Malaria, L-Lactate Dehydrogenase, C-Reactive Protein, Protozoan Proteins, Antigens, Protozoan, Diagnostic Tests, Routine, Immunoassay, Sensitivity and Specificity, Endemic Diseases, Adult, Child, Child, Preschool, Infant, Uganda, Myanmar, Asymptomatic Infections, P. falciparum, P. vivax, diagnostics, malaria, P. falciparum, P. vivax, Vector-Borne Diseases, Infectious Diseases, Rare Diseases, Clinical Research, 4.2 Evaluation of markers and technologies, 4.1 Discovery and preclinical testing of markers and technologies, Infection, Microbiology, Biological Sciences, Agricultural and Veterinary Sciences, Medical and Health Sciences
Abstract

Malaria rapid diagnostic tests (RDTs) primarily detect Plasmodium falciparum antigen histidine-rich protein 2 (HRP2) and the malaria-conserved antigen lactate dehydrogenase (LDH) for P. vivax and other malaria species. The performance of RDTs and their utility is dependent on circulating antigen concentration distributions in infected individuals in a population in which malaria is endemic and on the limit of detection of the RDT for the antigens. A multiplexed immunoassay for the quantification of HRP2, P. vivax LDH, and all-malaria LDH (pan LDH) was developed to accurately measure circulating antigen concentration and antigen distribution in a population with endemic malaria. The assay also measures C-reactive protein (CRP) levels as an indicator of inflammation. Validation was conducted with clinical specimens from 397 asymptomatic donors from Myanmar and Uganda, confirmed by PCR for infection, and from participants in induced blood-stage malaria challenge studies. The assay lower limits of detection for HRP2, pan LDH, P. vivax LDH, and CRP were 0.2 pg/ml, 9.3 pg/ml, 1.5 pg/ml, and 26.6 ng/ml, respectively. At thresholds for HRP2, pan LDH, and P. vivax LDH of 2.3 pg/ml, 47.8 pg/ml, and 75.1 pg/ml, respectively, and a specificity ≥98.5%, the sensitivities for ultrasensitive PCR-confirmed infections were 93.4%, 84.9%, and 48.9%, respectively. Plasmodium LDH (pLDH) concentration, in contrast to that of HRP2, correlated closely with parasite density. CRP levels were moderately higher in P. falciparum infections with confirmed antigenemia versus those in clinical specimens with no antigen. The 4-plex array is a sensitive tool for quantifying diagnostic antigens in malaria infections and supporting the evaluation of new ultrasensitive RDTs.

Published
2019

10. Clinical performance validation of the STANDARD G6PD test: A multi-country pooled analysis

Author

Adissu, Wondimagegn, primary, Brito, Marcelo, additional, Garbin, Eduardo, additional, Macedo, Marcela, additional, Monteiro, Wuelton, additional, Mukherjee, Sandip Kumar, additional, Myburg, Jane, additional, Alam, Mohammad Shafiul, additional, Bancone, Germana, additional, Bansil, Pooja, additional, Pal, Sampa, additional, Sharma, Abhijit, additional, Zobrist, Stephanie, additional, Bryan, Andrew, additional, Chu, Cindy S., additional, Das, Santasabuj, additional, Domingo, Gonzalo J., additional, Hann, Amanda, additional, Kublin, James, additional, Lacerda, Marcus V. G., additional, Layton, Mark, additional, Ley, Benedikt, additional, Murphy, Sean C., additional, Nosten, Francois, additional, Pereira, Dhélio, additional, Price, Ric N., additional, Talukdar, Arunansu, additional, Yilma, Daniel, additional, and Gerth-Guyette, Emily, additional

Published
2023
Full Text
View/download PDF

12. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types

Author

Golden, Allison, primary, Oliveira-Silva, Michelle, additional, Slater, Hannah, additional, Vieira, Alexia Martines, additional, Bansil, Pooja, additional, Gerth-Guyette, Emily, additional, Leader, Brandon T., additional, Zobrist, Stephanie, additional, Braga Ferreira, Alan Kennedy, additional, Santos de Araujo, Erika Crhistina, additional, de Lucena Cruz, Catherine Duran, additional, Garbin, Eduardo, additional, Bizilj, Greg T., additional, Carlson, Sean J., additional, Sagalovsky, Mariana, additional, Pal, Sampa, additional, Gupta, Vin, additional, Wolansky, Leo, additional, Boyle, David S., additional, Vieira Dall’Acqua, Deusilene Souza, additional, Naveca, Felipe Gomes, additional, do Nascimento, Valdinete Alves, additional, Villalobos Salcedo, Juan Miguel, additional, Drain, Paul K., additional, Costa, Alexandre Dias Tavares, additional, Pereira, Dhélio, additional, and Domingo, Gonzalo J., additional

Published
2023
Full Text
View/download PDF

15. Assessing the Operational Feasibility of Integrating Point-of-Care G6PD Testing into Plasmodium vivax Malaria Management in Vietnam

Author

Gerth-Guyette, Emily, primary, Nguyen, Huyen Thanh, additional, Nowak, Spike, additional, Hoang, Nga Thu, additional, Mai, Đặng Thị Tuyết, additional, Thị Sang, Vũ Thị, additional, Long, Nguyễn Đức, additional, Mvundura, Mercy, additional, Nguyen, Nhu, additional, Domingo, Gonzalo J., additional, and Phúc, Bùi Quang, additional

Published
2023
Full Text
View/download PDF

17. Clinical performance validation of the STANDARD G6PD Test: A multi-country pooled analysis

Author

Adissu, Wondimagegn, primary, Brito, Marcelo, additional, Garbin, Eduardo, additional, Macedo, Marcela, additional, Monteiro, Wuelton, additional, Mukherjee, Sandip, additional, Myburg, Jane, additional, Alam, Mohammad Shafiul, additional, Bancone, Germana, additional, Bansil, Pooja, additional, Pal, Sampa, additional, Sharma, Abhijit, additional, Zobrist, Stephanie, additional, Bryan, Andrew, additional, Chu, Cindy S, additional, Das, Santasabuj, additional, Domingo, Gonzalo J, additional, Hann, Amanda, additional, Kublin, James, additional, Lacerda, Marcus VG, additional, Layton, Mark, additional, Ley, Benedikt, additional, Murphy, Sean C, additional, Nosten, Francois, additional, Pereira, Dhélio, additional, Price, Ric N, additional, Talukdar, Arunansu, additional, Yilma, Daniel, additional, and Gerth-Guyette, Emily, additional

Published
2022
Full Text
View/download PDF

18. Correction: Quantification of glucose-6-phosphate dehydrogenase activity by spectrophotometry: A systematic review and meta-analysis

Author

Pfeffer, Daniel A., Ley, Benedikt, Howes, Rosalind E., Adu, Patrick, Alam, Mohammad Shafiul, Bansil, Pooja, Boum, Yap, Brito, Marcelo, Charoenkwan, Pimlak, Clements, Archie, Cui, Liwang, Deng, Zeshuai, Egesie, Ochaka Julie, Espino, Fe Esperanza, von Fricken, Michael E., Hamid, Muzamil Mahdi Abdel, He, Yongshu, Henriques, Gisela, Khan, Wasif Ali, Khim, Nimol, Kim, Saorin, Lacerda, Marcus, Lon, Chanthap, Mekuria, Asrat Hailu, Menard, Didier, Monteiro, Wuelton, Nosten, François, Oo, Nwe Nwe, Pal, Sampa, Palasuwan, Duangdao, Parikh, Sunil, Pasaribu, Ayodhia Pitaloka, Poespoprodjo, Jeanne Rini, Price, David J., Roca-Feltrer, Arantxa, Roh, Michelle E., Saunders, David L., Spring, Michele D., Sutanto, Inge, LeyThriemer, Kamala, Weppelmann, Thomas A., Seidlein, Lorenz von, Satyagraha, Ari Winasti, Bancone, Germana, Domingo, Gonzalo J., and Price, Ric N.

Subjects
Phosphates -- Analysis, Glucose -- Analysis, Biological sciences
Abstract

Author(s): Daniel A. Pfeffer, Benedikt Ley, Rosalind E. Howes, Patrick Adu, Mohammad Shafiul Alam, Pooja Bansil, Yap Boum, Marcelo Brito, Pimlak Charoenkwan, Archie Clements, Liwang Cui, Zeshuai Deng, Ochaka Julie [...]

Published
2020
Full Text
View/download PDF

19. Quantitative G6PD Deficiency Screening in Routine Malaria Diagnostic Units in the Brazilian Amazon (SAFEPRIM): An Operational Mixed-Methods Study

Author

Brito-Sousa, Jose Diego, primary, Murta, Felipe, additional, Vitor-Silva, Sheila, additional, Sampaio, Vanderson, additional, Mendes, Maxwell, additional, Souza, Brenda, additional, Batista, Talita, additional, Santos, Alicia, additional, Marques, Leonardo, additional, Barbosa, Laila, additional, Balieiro, Patricia, additional, Silva-Neto, Alexandre, additional, Rabello, Renata, additional, Brito, Marcelo, additional, Silva, Emanuelle, additional, Rodovalho, Sheila, additional, Arcanjo, Ana Ruth, additional, Melo, Gisely, additional, Recht, Judith, additional, Domingo, Gonzalo J., additional, Valle, Suiane, additional, Souza, Rodrigo, additional, Nakagawa, Theresa, additional, Monteiro, Wuelton, additional, and Lacerda, Marcus, additional

Published
2022
Full Text
View/download PDF

20. Antigen concentration, viral load, and test performance for SARS-CoV-2 in multiple specimen types

Author

Golden, Allison, primary, Oliveira-Silva, Michelle, additional, Slater, Hannah, additional, Vieira, Alexia Martines, additional, Bansil, Pooja, additional, Gerth-Guyette, Emily, additional, Leader, Brandon T., additional, Zobrist, Stephanie, additional, Braga Ferreira, Alan Kennedy, additional, Santos de Araujo, Erika Crhistina, additional, de Lucena Cruz, Catherine Duran, additional, Garbin, Eduardo, additional, Bizilj, Greg T., additional, Carlson, Sean J., additional, Sagalovsky, Mariana, additional, Pal, Sampa, additional, Gupta, Vin, additional, Wolansky, Leo, additional, Boyle, David S., additional, Vieira Dall’Acqua, Deusilene Souza, additional, Naveca, Felipe Gomes, additional, do Nascimento, Valdinete Alves, additional, Villalobos Salcedo, Juan Miguel, additional, Drain, Paul K., additional, Costa, Alexandre Dias Tavares, additional, Pereira, Dhélio, additional, and Domingo, Gonzalo J, additional

Published
2022
Full Text
View/download PDF

21. Genetic Variants of Glucose-6-Phosphate Dehydrogenase and Their Associated Enzyme Activity: A Systematic Review and Meta-Analysis

Author

Pfeffer, Daniel A., primary, Satyagraha, Ari Winasti, additional, Sadhewa, Arkasha, additional, Alam, Mohammad Shafiul, additional, Bancone, Germana, additional, Boum, Yap, additional, Brito, Marcelo, additional, Cui, Liwang, additional, Deng, Zeshuai, additional, Domingo, Gonzalo J., additional, He, Yongshu, additional, Khan, Wasif A., additional, Kibria, Mohammad Golam, additional, Lacerda, Marcus, additional, Menard, Didier, additional, Monteiro, Wuelton, additional, Pal, Sampa, additional, Parikh, Sunil, additional, Roca-Feltrer, Arantxa, additional, Roh, Michelle, additional, Sirdah, Mahmoud M., additional, Wang, Duoquan, additional, Huang, Qiuying, additional, Howes, Rosalind E., additional, Price, Ric N., additional, and Ley, Benedikt, additional

Published
2022
Full Text
View/download PDF

28. Screening for Severe Acute Respiratory Syndrome Coronavirus 2 in Close Contacts of Individuals With Confirmed Infection: Performance and Operational Considerations

Author

Zobrist, Stephanie, primary, Oliveira-Silva, Michelle, additional, Vieira, Alexia Martines, additional, Bansil, Pooja, additional, Gerth-Guyette, Emily, additional, Leader, Brandon T, additional, Golden, Allison, additional, Slater, Hannah, additional, Cruz, Catherine Duran de Lucena, additional, Garbin, Eduardo, additional, Sagalovsky, Mariana, additional, Pal, Sampa, additional, Gupta, Vin, additional, Wolansky, Leo, additional, Dall’Acqua, Deusilene Souza Vieira, additional, Naveca, Felipe Gomes, additional, Nascimento, Valdinete Alves do, additional, Salcedo, Juan Miguel Villalobos, additional, Drain, Paul K, additional, Costa, Alexandre Dias Tavares, additional, Domingo, Gonzalo J, additional, and Pereira, Dhélio, additional

Published
2022
Full Text
View/download PDF

29. Additional file 1 of The in-vivo dynamics of Plasmodium falciparum HRP2: implications for the use of rapid diagnostic tests in malaria elimination

Author

Marquart, Louise, Webb, Lachlan, O’Rourke, Peter, Gatton, Michelle L., Hsiang, Michelle S., Kalnoky, Michael, Jang, Ihn Kyung, Ntuku, Henry, Mumbengegwi, Davis R., Domingo, Gonzalo J., McCarthy, James S., and Britton, Sumudu

Abstract

Additional file 1: Material S1. Anti-malarial treatment details for IBSM individuals. Table S1. Antimalarial treatment given for the 15 IBSM individuals from four different studies, as identified with the clinical trial name and clinical trial ID. The antimalarial and dose given at day of treatment on Day 7 is detailed for each subject. Material S2. Methods to fit PfHRP2 model to the 6 individuals from the Namibia longitudinal cohort study. Table S2. Model performance of the Base model and red the Final model for the 15 IBSM individuals. Performance measures were the number (%) of PfHRP2 observations within the range of the predicted minimum and maximum PfHRP2 concentrations, the residual sum of squares (RSS) and the residual mean sum of square (RMSE). Table S3. Model performance of the updated model with PfHRP2 half-life estimate of 1.67 days applied to 6 individuals aged between 23 and 27 years from the study in Namibia in participants with Plasmodium falciparum mono-infection. Performance measures were the number (%) of PfHRP2 observations within the range of the predicted minimum and maximum PfHRP2 concentrations, the residual sum of squares (RSS), residual mean sum of square (RMSE), the predicted and observed days above 800 or 80 pg/mL. Figure S1. Fits of the Base Model and the Final Model for the 15 IBSM individuals. The observed parasitemia over the course of infection is represented by black solid line, observed PfHRP2 concentration is represented by circles (pre-treatment in solid circles and post-treatment in open circles), and the predicted minimum and maximum PfHRP2 concentration from the model is shown as blue and red dashed lines, respectively. Figure S2. Simulated parasitemia growth and clearance over the course of the infection for each of the 6 individuals from the Namibia longitudinal cohort study is represented by the black line. The observed parasitemia (parasites/mL) during the study are represented by the blue triangles and the replicating parasites used as input into the PfHRP2 model are represented by open circles. Figure S3. Fits of the final model with elimination half-life of 1.67 days to the 6 individuals from the Namibia longitudinal cohort study, with observed PfHRP2 concentration represented by closed circles, and minimum and maximum predicted PfHRP2 concentration represented by the blue and red dashed lines, respectively. The dashed grey horizontal line represents the threshold of 800 pg/mL and the solid grey horizontal line represents the threshold of 80 pg/mL which correspond to the positivity threshold of an RDT and usRDT respectively.

Published
2022
Full Text
View/download PDF

30. Additional file 1 of Performance and utility of more highly sensitive malaria rapid diagnostic tests

Author

Slater, Hannah C., Ding, Xavier C., Knudson, Sophia, Bridges, Daniel J., Moonga, Hawela, Saad, Neil J., De Smet, Martin, Bennett, Adam, Dittrich, Sabine, Slutsker, Laurence, and Domingo, Gonzalo J.

Subjects
parasitic diseases, equipment and supplies, health care economics and organizations
Abstract

Additional file 1. Estimating population-level prevalence by HS-RDT and PCR in Owalla et al.

Published
2022
Full Text
View/download PDF

31. Additional file 1 of Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

Author

Jang, Ihn Kyung, Jiménez, Alfons, Rashid, Andrew, Barney, Rebecca, Golden, Allison, Ding, Xavier C., Domingo, Gonzalo J., and Mayor, Alfredo

Subjects
parasitic diseases
Abstract

Additional file 1: Table S1. Comparison of two multiplex platforms for the quantitative assessment of malaria antigens. Table S2. Characteristics of the Q-Plex and xMAP assays. Table S3. Linear relation between the WHO international standard P. falciparum and P. vivax antigens and assay concentration results in whole blood or DBS. Regression analysis showed the fit of the data with R2 values that met the acceptance criteria (R2 ≥ 0.85). One operator conducted three experiments over a week. Figure S1. HRP2 concentration in (A) whole blood and (B) DBS plotted against the parasitemia. Three subsets of dilution-series samples composed of different P. falciparum laboratory strains (W2, Dd2, and 3BD5) were tested by using the Q-Plex and xMAP. Measured concentration was plotted on the y-axis against parasitemia (par/mL) on the x-axis. Red and blue symbols and regression lines represent samples spiked with different strains and test types. Figure S2. Correlation analysis. Correlation plots of antigen concentration corrected against the WHO international standard P. falciparum and P. vivax antigens, P. falciparum culture, and clinical samples in (A) whole blood and (B) DBS. The Pearson correlation r value and the equation of the regression line obtained from analysis using log transformed data are shown. Outlier sample sets were color-coded.

Published
2022
Full Text
View/download PDF

35. Repeatability and reproducibility of a handheld quantitative G6PD diagnostic

Author

Ley, Benedikt, primary, Winasti Satyagraha, Ari, additional, Kibria, Mohammad Golam, additional, Armstrong, Jillian, additional, Bancone, Germana, additional, Bei, Amy K., additional, Bizilj, Greg, additional, Brito, Marcelo, additional, Ding, Xavier C., additional, Domingo, Gonzalo J., additional, von Fricken, Michael E., additional, Gornsawun, Gornpan, additional, Lam, Brandon, additional, Menard, Didier, additional, Monteiro, Wuelton, additional, Ongarello, Stefano, additional, Pal, Sampa, additional, Panggalo, Lydia Visita, additional, Parikh, Sunil, additional, Pfeffer, Daniel A., additional, Price, Ric N., additional, da Silva Orfano, Alessandra, additional, Wade, Martina, additional, Wojnarski, Mariusz, additional, Worachet, Kuntawunginn, additional, Yar, Aqsa, additional, Alam, Mohammad Shafiul, additional, and Howes, Rosalind E., additional

Published
2022
Full Text
View/download PDF

36. Screening for SARS-CoV-2 in close contacts of individuals with confirmed infection: performance and operational considerations

Author

Zobrist, Stephanie, primary, Oliveira-Silva, Michelle, additional, Vieira, Alexia Martines, additional, Bansil, Pooja, additional, Gerth-Guyette, Emily, additional, Leader, Brandon T, additional, Golden, Allison, additional, Slater, Hannah, additional, Cruz, Catherine Duran de Lucena, additional, Garbin, Eduardo, additional, Sagalovsky, Mariana, additional, Pal, Sampa, additional, Gupta, Vin, additional, Wolansky, Leo, additional, Dall Acqua, Deusilene Souza Vieira, additional, Naveca, Felipe Gomes, additional, Nascimento, Valdinete Alves do, additional, Villalobos Salcedo, Juan Miguel, additional, Drain, Paul K, additional, Costa, Alexandre Dias Tavares, additional, Domingo, Gonzalo J, additional, and Pereira, Dhelio, additional

Published
2022
Full Text
View/download PDF

38. Reference and point-of-care testing for G6PD deficiency: Blood disorder interference, contrived specimens, and fingerstick equivalence and precision

Author

Pal, Sampa, primary, Myburgh, Jane, additional, Bansil, Pooja, additional, Hann, Amanda, additional, Robertson, Lynn, additional, Gerth-Guyette, Emily, additional, Ambler, Gwen, additional, Bizilj, Greg, additional, Kahn, Maria, additional, Zobrist, Stephanie, additional, Manis, Michelle R., additional, Styke, Nickolas A., additional, Allan, Vajra, additional, Ansbro, Richard, additional, Akingbade, Tobi, additional, Bryan, Andrew, additional, Murphy, Sean C., additional, Kublin, James G., additional, Layton, Mark, additional, and Domingo, Gonzalo J., additional

Published
2021
Full Text
View/download PDF

39. Evaluation of a point-of-care diagnostic to identify glucose-6-phosphate dehydrogenase deficiency in Brazil

Author

Zobrist, Stephanie, primary, Brito, Marcelo, additional, Garbin, Eduardo, additional, Monteiro, Wuelton M., additional, Clementino Freitas, Suellen, additional, Macedo, Marcela, additional, Soares Moura, Aline, additional, Advani, Nicole, additional, Kahn, Maria, additional, Pal, Sampa, additional, Gerth-Guyette, Emily, additional, Bansil, Pooja, additional, Domingo, Gonzalo J., additional, Pereira, Dhelio, additional, and Lacerda, Marcus VG, additional

Published
2021
Full Text
View/download PDF

40. Screening Antibodies Raised against the Spike Glycoprotein of SARS-CoV-2 to Support the Development of Rapid Antigen Assays

Author

Cantera, Jason L., primary, Cate, David M., additional, Golden, Allison, additional, Peck, Roger B., additional, Lillis, Lorraine L., additional, Domingo, Gonzalo J., additional, Murphy, Eileen, additional, Barnhart, Bryan C., additional, Anderson, Caitlin A., additional, Alonzo, Luis F., additional, Glukhova, Veronika, additional, Hermansky, Gleda, additional, Barrios-Lopez, Brianda, additional, Spencer, Ethan, additional, Kuhn, Samantha, additional, Islam, Zeba, additional, Grant, Benjamin D., additional, Kraft, Lucas, additional, Herve, Karine, additional, de Puyraimond, Valentine, additional, Hwang, Yuri, additional, Dewan, Puneet K., additional, Weigl, Bernhard H., additional, Nichols, Kevin P., additional, and Boyle, David S., additional

Published
2021
Full Text
View/download PDF

41. Usability of a point-of-care diagnostic to identify glucose-6-phosphate dehydrogenase deficiency: a multi-country assessment of test label comprehension and results interpretation

Author

Gerth-Guyette, Emily, primary, Adissu, Wondimagegn, additional, Brito, Marcelo, additional, Garbin, Eduardo, additional, Macedo, Marcela, additional, Sharma, Abhijit, additional, Das, Santasabuj, additional, Lacerda, Marcus V. G., additional, Pereira, Dhélio, additional, Talukdar, Arunansu, additional, Yilma, Daniel, additional, Pal, Sampa, additional, Zobrist, Stephanie, additional, and Domingo, Gonzalo J., additional

Published
2021
Full Text
View/download PDF

42. Cost analysis of centralized viral load testing for antiretroviral therapy monitoring in Nicaragua, a low-HIV prevalence, low-resource setting

Author

Gerlach, Jay, Sequeira, Magda, Alvarado, Vivian, Cerpas, Christian, Balmaseda, Angel, Gonzalez, Alcides, Santos, Tala, Levin, Carol E., Amador, Juan Jose, and Domingo, Gonzalo J.

Subjects
Antiviral agents -- Patient outcomes, Viremia -- Measurement, Drug utilization -- Economic aspects, Health
Abstract

Background: HIV viral load testing as a component of antiretroviral therapy monitoring is costly. Understanding the full costs and the major sources of inefficiency associated with viral load testing is critical for optimizing the systems and technologies that support the testing process. The objective of our study was to estimate the costs associated with viral load testing performed for antiretroviral therapy monitoring to both patients and the public healthcare system in a low‐HIV prevalence, low‐resource country. Methods: A detailed cost analysis was performed to understand the costs involved in each step of performing a viral load test in Nicaragua, from initial specimen collection to communication of the test results to each patient's healthcare provider. Data were compiled and cross referenced from multiple information sources: laboratory records, regional surveillance centre records, and scheduled interviews with the key healthcare providers responsible for HIV patient care in five regions of the country. Results: The total average cost of performing a viral load test in Nicaragua varied by region, ranging from US$99.01 to US$124.58, the majority of which was at the laboratory level: $88.73 to $97.15 per specimen, depending on batch size. The average cost to clinics at which specimens were collected ranged from $3.31 to $20.92, depending on the region. The average cost per patient for transportation, food, lodging and lost income ranged from $3.70 to $14.93. Conclusions: The quantitative viral load test remains the single most expensive component of the process. For the patient, the distance of his or her residence from the specimen collection site is a large determinant of cost. Importantly, the efficiency of results reporting has a large impact on the cost per result delivered to the clinician and utility of the result for patient monitoring. Detailed cost analysis can identify opportunities for removing barriers to effective antiretroviral therapy monitoring programmes in limited‐resource countries with low HIV prevalence., Background Recent progress in managing antiretroviral therapy (ART) in patients with HIV/AIDS in low‐resource settings has outpaced the scaling‐up of tests to support effective case management. CD4 and HIV viral [...]

Published
2010
Full Text
View/download PDF

43. Real-life implementation of a G6PD deficiency screening qualitative test into routine vivax malaria diagnostic units in the Brazilian Amazon (SAFEPRIM study)

Author

Brito-Sousa, Jose Diego, primary, Murta, Felipe, additional, Vitor-Silva, Sheila, additional, Sampaio, Vanderson S., additional, Mendes, Maxwell O., additional, Brito, Marcelo A. M., additional, Batista, Talita S. B., additional, Santos, Alicia P. C., additional, Marques, Leonardo L. G., additional, Barbosa, Laila R. A., additional, Melo, Marly M., additional, Baia-da-Silva, Djane C., additional, Silva-Neto, Alexandre V., additional, Santos, Thalie C., additional, Souza, Brenda K. A., additional, Figueiredo, Erick F. G., additional, Silva, Emanuelle L., additional, Rodovalho, Sheila, additional, Nakagawa, Theresa H., additional, Arcanjo, Ana Ruth, additional, Siqueira, André M., additional, Melo, Gisely C., additional, Recht, Judith, additional, Domingo, Gonzalo J., additional, Bassat, Quique, additional, Bancone, Germana, additional, Monteiro, Wuelton M., additional, and Lacerda, Marcus V. G., additional

Published
2021
Full Text
View/download PDF

45. Additional file 1 of Ultra-sensitive RDT performance and antigen dynamics in a high-transmission Plasmodium falciparum setting in Mali

Author

Reichert, Emily N., Hume, Jen C. C., Issaka Sagara, Healy, Sara A., Mahamadoun H. Assadou, Merepen A. Guindo, Barney, Rebecca, Rashid, Andy, Ihn Kyung Yang, Golden, Allison, Domingo, Gonzalo J., Duffy, Patrick E., and Slater, Hannah C.

Subjects
parasitic diseases
Abstract

Additional file 1: Figure S1. Scatterplot showing correlation between parasite count by microscopy and HRP2 concentration quantified by Q-Plex ELISA (R2 = 0.29) for all samples P. falciparum positive by microscopy (> 0 parasites/µL). Abbreviations: ELISA, enzyme-linked immunosorbent assay; HRP2, histidine rich protein 2; pLDH, Plasmodium lactate dehydrogenase. Figure S2. Scatterplot showing correlation between parasite count by microscopy and pLDH concentration quantified by Q-Plex ELISA (R2 = 0.61) for all samples P. falciparum positive by microscopy (> 0 parasites/µL). Table S1. Antigen ratios by direct skin feed (DSF) infectivity status. Geometric means for the two antigens of interest, HRP2 and pLDH, along with the geometric mean HRP2:pLDH ratio for study participants that were DSF-positive (n = 11) or DSF-negative (n = 603), grouped by microscopy result. Individuals positive by DSF had a significantly lower HRP2:pLDH ratio (p = 0.02) on average in reference to DSF and microscopy negative individuals. Abbreviations: geo, geometric; HRP2, histidine rich protein 2; pLDH, Plasmodium lactate dehydrogenase; SD, standard deviation.

Published
2020
Full Text
View/download PDF

46. An Experimental Human Blood-Stage Model for Studying Plasmodium malariae Infection

Author

Woodford, John, Collins, K.A., Odedra, Anand, Wang, Claire, Jang, Ihn Kyung, Domingo, Gonzalo J., Otto, Thomas D., McCarthy, James S., Woodford, John, Collins, K.A., Odedra, Anand, Wang, Claire, Jang, Ihn Kyung, Domingo, Gonzalo J., Otto, Thomas D., and McCarthy, James S.

Abstract

Contains fulltext : 217369.pdf (Publisher’s version ) (Open Access)

Published
2020

48. A conserved cis peptide bond is necessary for the activity of Bowman-Birk inhibitor protein

Author

Brauer, Arnd B.E., Domingo, Gonzalo J., Cooke, Robert M., Matthews, Stephen J., and Leatherbarrow, Robin J.

Subjects
Structure-activity relationships (Biochemistry) -- Analysis, Proteins -- Structure, Proteases -- Analysis, Chemical bonds -- Influence, Biological sciences, Chemistry
Abstract

Substitution of proline residues with alanine in the P3'-P4' cis-Pro-trans-Pro sequence of the Bowman-Birk inhibitor family of protease inhibitors reveals that the proline at P3' and a cis peptide bond at that position are essential for the biological activity. Further, P4' stabilizes the cis conformation.

Published
2002

Catalog

Books, media, physical & digital resources
See catalog results