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Your search keyword '"Doireann Gordon"' showing total 3 results
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3 results on '"Doireann Gordon"'

1. A fluorogenic probe for granzyme B enables in-biopsy evaluation and screening of response to anticancer immunotherapies

Author

Jamie I. Scott, Lorena Mendive-Tapia, Doireann Gordon, Nicole D. Barth, Emily J. Thompson, Zhiming Cheng, David Taggart, Takanori Kitamura, Alberto Bravo-Blas, Edward W. Roberts, Jordi Juarez-Jimenez, Julien Michel, Berber Piet, I. Jolanda de Vries, Martijn Verdoes, John Dawson, Neil O. Carragher, Richard A. O’ Connor, Ahsan R. Akram, Margaret Frame, Alan Serrels, and Marc Vendrell

Subjects
Science
Abstract

Granzyme B is found in activated T cells and can be used as a marker of T cell activation. Here, the authors generate a fluorescent probe that can detect Granzyme B levels in tumours, and has the potential to be used as a biomarker of response to immunotherapy.

Published
2022
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3. A Palette of Minimally Tagged Sucrose Analogues for Real‐Time Raman Imaging of Intracellular Plant Metabolism

Author

Ailsa Geddis, Karl Oparka, Marc Vendrell, Anke Reinders, Martin Lee, Kirsten Knox, William J. Tipping, Fabio de Moliner, Doireann Gordon, and John M. Ward

Subjects
Azides, Sucrose, Cell Membrane Permeability, Imaging Agents, Spectrum Analysis, Raman, 010402 general chemistry, 01 natural sciences, Catalysis, chemistry.chemical_compound, symbols.namesake, Coumarins, Plant Cells, Yeasts, Moiety, QD, Plant Proteins, Microscopy, Chemistry, 010405 organic chemistry, Communication, imaging, Membrane Transport Proteins, Fructose, Transporter, General Chemistry, General Medicine, Plant cell, Communications, 0104 chemical sciences, Kinetics, Biochemistry, sugars, Alkynes, transport, symbols, Metabolome, stimulated Raman scattering, Indicators and Reagents, Raman spectroscopy, probes, Intracellular, Raman scattering
Abstract

Sucrose is the main saccharide used for long‐distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real‐time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne‐tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real‐time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy., We describe a chemical strategy to prepare sucrose derivatives with vibrational tags and the characterisation of the first optical analogue of sucrose able to image real‐time trafficking of sucrose in live plant cells.

Published
2021
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