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Uroleucon australe Nieto Nafría & Mier Durante, sp. n. Types. Holotype: apterous viviparous female, CHILE, Aysén Region, Capitán Prat province, Villa O’Higgins: La Baranda (48º 15’ S, 72º 21’ W, 430 m a.s.l.), on Adesmia boronioides; 18-January-2019; Mier Durante, Nieto Nafría and Ortego leg., sample CHI-546, specimen number 2 on a slide with one paratype; collection of the University of León (León, Spain). Paratypes (9 viviparous apterous females, 49 oviparous females and 11 males): CHILE, same data as that of the holotype, 6 apterous viviparous females and 4 oviparous females; CHILE, Aysén Region, General Carrera province, General Carrera lake South bank (46º 37’ S, 72º 21’ W, 520 m a.s.l.), 20-January-2019, 2 apterous viviparous females; CHILE, Magallanes Region, Última Esperanza province, Sarmiento de Gamboa lake South bank (51º 02’ S, 72º 47’ W, 160 m a.s.l.), 5-February-2016, 6 oviparous females and 4 males; CHILE, Última Esperanza province, Cerro Castillo 5 km South (51º 17’ S, 72º 24’ W, 140 m a.s.l.), 6-February-2016, 1 viviparous apterous female, 39 oviparous females and 7 males; all them on Adesmia boronioides, Mier Durante, Nieto Nafría and Ortego leg.; collections of the University of León (León, Spain), Jaime Ortego (Mendoza, Argentina) and The Natural History Museum (London, United Kingdom). Apterous viviparous females. Figures 1 D–1I. Based on 10 specimens. Colour when alive matte green due to the presence of whitish waxy powder. Body 3.23–3.70 mm long and pear-shaped with long antennae and legs and noticeable siphunculi and cauda. Mounted specimens more-or-less very light brown with light brown to blackishbrown head, appendages, siphunculi, genital and anal plates and cauda, see details below. Setae on dorsum of body and antennae and several on legs thick and with apex truncate; other setae more-or-less pointed. Marginal tubercles absent. Head, including clypeus and mandibular and maxillary laminae, medium-brown. Frons sinuous, medialfrontal tubercle lower than the frontal-lateral (or antennal) tubercles. Cephalic dorsum with two pale anterior setae and four pale posterior setae. Antennal segment I darker than head, mainly on its inner side, and dorsally striate; antennal segment II pigmented as the head and weakly ornamented on its dorsal side; antennal segment III nearly smooth and progressively darkened from base, pigmented like segment II to a blackish brown extreme distal portion; antennal segment IV with transverse marks, and dark brown with a distal portion blackish brown; segments V and VI imbricated and dark brown. Sensoria round and with thick walls; primary sensoria ciliate; secondary sensoria small, non-protruding, more-or-less aligned from the end of the paler part of the segment. Rostrum reaching past middle coxae; proximal rostral segments (I and II) are pigmented as head and as middle strip of segment III, whose sides are very pigmented, almost black, as much as the ultimate segment (= rostral segments IV+V in Blackman & Eastop 2019), which is narrow. In the legs, the dominant pigmentation is similar to that of the head, except for areas internal-proximal of coxae, dorsal-distal of femora and distal about one eighth of tibiae, and entire tarsi, which can be darker up to blackish brown. Leg segments are smooth, except for tarsi. Thoracic and abdominal dorsum with shriveled appearance and no more pigmented than the cephalic dorsum, with small, brown spiracular and intersegmental-muscular-sclerites only present on thorax. Setiferous sclerites are completely absent in several specimens, in the most “ornamented” specimens they are present on abdominal segments 6 to 8 and on marginal areas of segments 3 and 4, although not associated with all setae; note that thoracic and abdominal setiferous sclerites are frequent, wide and well pigmented in most Uroleucon, but usually absent in species living on Adesmia. Siphunculi are progressively pigmented from medium-brown to dark brown, cylindrical and more-or-less as thick as the hind tibiae on most of their length, enlarged at its base, trumpet-shaped at the apex, with very small flange, and with small polygonal cells arranged in 8 to 10 rows and much more clearly defined than those of U. adesmiae and U. nahuelhuapense. Subgenital plate bicolored, being paler on the lateral-posterior edge. Anal plate as dark as the darkest part of siphunculi. Cauda lanceolate, more-or-less blunt at the apex, no darker than the palest part of siphunculi, with long and delicate setae. Metric and meristic features are in Table 3. Alate viviparous females. Unknown. Oviparous females. Figure 3D. Based on 49 specimens (24 measured, taken from all samples). Very similar to viviparous apterous females, with the following differences: (1) bright green when alive; (2) hind tibia somewhat swollen on proximal third with scent plates extending distally beyond the swollen portion; (3) pigmented zones of appendages paler, especially in specimens from Magallanes region, which have no brown areas on coxae and femora; (4) siphunculi paler and not so trumpet-shaped, presenting a slight flange; (5) cauda also paler. Quantitative features are in Table 3. Males. Figures 3E and 3F. Based on 11 specimens. Apterous. Brown when alive. They are also very similar to apterous viviparous females, although slightly smaller, with siphunculi shorter than viviparae and oviparae, less markedly trumped-shaped and with a more-or-less marked flange, and cauda also shorter and more-or-less triangular. Scent plates are present in the hind tibiae, which are very slightly thickened than the tibiae of the other legs; the presence of these pheromonal structures is very surprising because they are typical of oviparous females in Aphidinae. Mounted specimens show: an intense pigmentation of antennal segments III to VI; abundant dorsal setiferous sclerites on thoracic and abdominal segments as pigmented as the intersegmental and spiracular sclerites, those on segment 8 coalescing in some specimens; a transverse band on segment 7; and sometimes few and very scattered ventral sclerites on other abdominal segments. Quantitative data are in Table 3. ......continued on the next page Etymology. The specific name australe is an epithet in neutral gender because Uroleucon is neutral, that means “belonging or relative to the south” and refers to the very southern distribution of the species. Bionomics. Uroleucon australe feeds on the small stems of small plants of Adesmia boronioides Hook. f. (Fabaceae), which is its only known host. It can be conjectured that the alate viviparae in this species are infrequent, because they and their nymphs are unknown, and alatae are also infrequent in the other two Uroleucon species living on Adesmia,. Males and oviparous females have been collected in the middle of summer, which shows that the life cycle of the species is abbreviated. As the males are apterous, it is clear that the species is monoecious, not host-alternating. The presence of scent plates in several apterous viviparous females is not surprising if they belong to autumnal generations. All studied males bear scent plates, but it is not possible to ensure that this is a universal character of the U. australe males, because the studied specimens were collected in two locations very close to each other. Geographical distribution. The new species is currently known in four Chilean localities with low altitude and south to parallel 46º S, in the Aysén and Magallanes regions, north and south of the Patagonian ice fields, which are connected in viable ways for the movement of insects in general by two corridors east and west of those fields. The eastern corridor has characteristics of climate and vegetation that are more similar to those of the collection sites, whilst the western corridor, formed by islands and fjords, is very humid and forested. Thus, it is possible that the species could also be found in Chubut and Santa Cruz (Argentina) in areas near the mountain range. Molecular analyses. Comparisons of U. australe sp. n. COI and tRNAleu-COII sequences with other South American Uroleucon, as well as northern hemisphere species, confirmed that U. australe was genetically most similar to U. nahuelhuapense (results not shown). Genetic distances between U. australe and U. nahuelhuapense were 1.7% for COI and 1.1% for tRNAleu-COII. These distances are within the range found previously for closely related aphid species (Foottit et al. 2008; Nieto Nafría et al. 2019), and corroborate the distinction of U. australe sp. n. based on morphology. Taxonomic discussion. The differences between Uroleucon species are usually based on the qualitative and quantitative characters exhibited by the respective apterous viviparous females; in fact, the keys to Uroleucon species by Blackman & Eastop (2019), and the successive keys to identify the South American Uroleucon species (de Carvalho et al. 1998; Mier Durante and Ortego 2008; Nieto Nafría et al. 2019) refer to characteristics of this morph, which may be the only one known, although features of alate viviparae are complementarily used in several disjunctive couplets. Apterae of several South American Uroleucon species differ from related species by some characteristics, sometimes very few, which can overlap in part, such as in the differences between U. bereticum (Blanchard), U. macolai (Blanchard) and U. mendocinum Mier Durante and Ortego. The same occurs in other species-rich aphid genera, for example, between specimens of Aphis fabae Scopoli, A. rumicis Linnaeus and A. solanella Theobald, or even in genera with few species, for example between apterous viviparae of Hyperomyzus lactucae (Linnaeus) and of H. pallidus Hille Ris Lambers. To carry out the identifications in these cases, several characters with overlapping ranges, sometimes extensive, must be studied in a large series of specimens. However, sometimes a solitary character can resolve the identification, even if it is qualitative and infrequently used for the identification of aphids of that group. Apterous viviparous females of U. nahuelhuapense and U. australe sp. n. are very similar to each other in quantitative features, and the only appreciable differences between them, although largely overlapping, are listed in Table 4. These differences have to be assessed with caution because the quantity of samples studied is small and their geographical origin is limited or very limited. However, the two species can be separated by two very obvious qualitative characteristics: body colour when alive and shape of siphunculi; apterous viviparous females of U. nahuelhuapense are bright green whereas those of U. australe sp. n. are matte green, and siphunculi of U. nahuelhuapense are subconical (Figure 1B), whereas those of U. australe sp. n. are distally trumpet-shaped. (Figure 1G). In certain cases, alate viviparous females assist in distinguishing some South American Uroleucon species, but the alatae of U. australe sp. n. are not known. On the contrary, oviparous females and males of U. nahuelhuapense and U. australe sp. n. are known and they help with taxonomic separation of the two species. The contribution of oviparous females to identification is small, because like apterous viviparae the oviparae of the two species are very similar. Useful features of oviparae in U. nahuelhuapense and U. australe sp. n. are listed in Table 4. Contrary to what is typical for aphids, the males of U. australe sp. n. have a greater number of distinguishing characters than are found in the females, particularly the viviparous females. Several differences between males of both species (Table 4) refer to the siphunculi and the cauda, which greatly influence the general appearance of these specimens and allow us to easily separate them (Figures 3B and 3E). The key by Nieto Nafría et al. (2019) can be used to identify apterous viviparous females of U. australe sp. n. after minor adjustments in disjunctive couplets 13, 14 and 15, which are in bold and underlined font, and including the new disjunctive couplet 15’, as follows: 13. Siphunculi usually at least 1.5 times cauda, which is 0.24−0.47 mm and can have 13 or less setae or 17– 41 setae, if more than 17 then ultimate rostral segment 1.1–1.4 times hind tarsi second segment, and antennal segment VI processus terminalis at most 4. 6 times antennal segment VI base and antennal segments I and II paler than most of the antennal flagellum............ 14 - {without changes} Siphunculi usually at most 1.6 times cauda, which is 0.30−0.83 mm and has 9−31 setae............. 18 14 {without changes} First tarsal segments with 3 setae. Siphunculi shorter than 1.8 times caudal length. [Antennal segment III with 2–8 secondary sensoria in apterae (restricted to proximal half) and 6–22 in alatae]. Pale to medium-green in life. On Baccharis species and Hysterionica jasionoides. Argentina: Catamarca, La Rioja, Mendoza, San Juan; Chile: Coquimbo.................................................................................................... U. essigi - First tarsal segments usually with 4–5 setae, if only 3 setae present on some legs then siphunculi 1.6 –2.3 times cauda.... 15 15 Apterae with 2– 15 secondary sensoria restricted to proximal 53% of the length of segment. On Adesmia boronioides. [ Anten- nal segment VI processus terminalis 3.2– 4.6 times antennal segment VI base; ultimate rostral segment 1.1–1.4 times hind tarsi second segment, which is 0.13– 0-18 mm; siphunculi 1.5 –2.3 times cauda, which is 0.33 –0.47 mm. Setae on antennal segment III 28– 53 μm ].......................................................................................15’ - {without changes} Apterae with 4–16 secondary sensoria extending over most of length of segment. Other above-mentioned characters are not presented together. On species of Asteraceae................................................ 16 15’ Distal part of siphunculi cylindrical and with a conspicuous terminal flange. Bright emerald green in life. Cauda with 17–29 setae. Siphunculus usually more 9.1–15.2 (exceptionally down to 8.4) times its width at the beginning of reticulation. Second segment of hind tarsi 0.13–0.17 mm. 10–21 dorsal setae on abdominal segments 2 to 5, being the spinal ones 23–50 μm long. Argentina: Neuquén..................................................................... U. nahuelhuapense - Distal part of siphunculi trumpet-shaped and with a small terminal flange. Matte green with whitish was powder in life. Cauda with 26–41 setae. Siphunculus 8.3–11.3 times its width at the beginning of reticulation. Second segment of hind tarsi 0.16–0.18 mm. 12–15 dorsal setae on abdominal segments 2 to 5, being the spinal ones 25–38 μm long. Chile: Aysén and Magallanes......................................................................................... U. australe sp.n.

Durante, M. Pilar Mier, Ortego, Jaime, Von Dohlen, Carol D., Nafría, Juan Manuel Nieto (2020): A further contribution to the knowledge of Uroleucon species (Hemiptera, Aphididae) living on Adesmia (Fabaceae) in southern South America, with description of a new species from Chile. Zootaxa 4748 (3): 548-560, DOI: https://doi.org/10.11646/zootaxa.4748.3.8

Uroleucon adesmiae Mier Durante & Ortego, 2008 Studied new material. ARGENTINA: Chubut province, Cushamen department, Epuyén: near 40 and 70 roads cross (42º 12’ S, 71º 23’ W, 380 m a.s.l.), on Adesmia boronioides, 12-January-2019, Mier Durante, Nieto Nafría and Ortego leg., 8 apterous viviparous females [6 measured], collection of the University of León (León, Spain). The apterous viviparous females collected in Epuyén are very similar to those previously studied (Mier Durante et al. 2008; Nieto Nafría et al. 2019), although with small variations in the limits of the ranges of several quantitative characteristics; most noteworthy variations are in Table 1. The latitude of Epuyén is intermediate between the latitudes of the known extremes of distribution of the species. Uroleucon adesmiae had until now been recorded from one or several species of Adesmia, which were not identified to species due to their stage of growth; however, they were clearly not A. boronioides. They were most likely one or several species of Adesmia recorded from the corresponding areas of the Neuquén and Chubut provinces by Ulibarri & Burkart (2000). From all these data and those available on U. nahuelhuapense and U. australe (see below) it can be concluded that U. adesmiae has the widest distribution and diversity of host plants of the three species of Uroleucon living on Adesmia., Published as part of Durante, M. Pilar Mier, Ortego, Jaime, Von Dohlen, Carol D. & Nafría, Juan Manuel Nieto, 2020, A further contribution to the knowledge of Uroleucon species (Hemiptera, Aphididae) living on Adesmia (Fabaceae) in southern South America, with description of a new species from Chile, pp. 548-560 in Zootaxa 4748 (3) on page 550, DOI: 10.11646/zootaxa.4748.3.8, http://zenodo.org/record/3701139, {"references":["Mier Durante, M. P. & Ortego, J. (2008) A new species of Uroleucon (Hemiptera: Aphididae) living on Adesmia (Fabaceae). Florida Entomologist, 91 (3), 367 - 371. https: // doi. org / 10.1653 / 0015 - 4040 (2008) 91 [367: ANSOUH] 2.0. CO; 2","Nieto Nafria, J. M., von Dohlen, C. D., Moreno-Gonzalez, V., Ortego, J. & Mier Durante, M. P. (2019) The species of Uroleucon (Hemiptera: Aphididae) living on Adesmia (Fabaceae) in Argentina, with description of a new species. Zootaxa, 4555 (4), 561 - 572. https: // doi. org / 10.11646 / zootaxa. 4555.4.7","Ulibarri, E. A. & Burkart, A. (2000) Sinopsis de las especies de Adesmia (Leguminosae, Adesmieae) de la Argentina. Darwiniana, 38 (1 - 2), 59 - 126. [http: // www. redalyc. org / articulo. oa? id = 66938208]"]}

Uroleucon nahuelhuapense Nieto Nafría & von Dohlen, 2019 Studied new material. ARGENTINA, Neuquén province, Los Lagos department, Santa María: km. 32 road 231 (40º 55’ S 71º 26’ W, 800 m a.s.l.) —species type locality—, on Adesmia boronioides, 10-January-2019, Ortego leg., 12 apterous viviparous females, 9 alate viviparous females, 19 oviparous females and 14 males, collections of the University of León (León, Spain) and The Natural History Museum (London, United Kingdom). Apterous viviparous females. Figures 1 A–1C. Mounted specimens of the new material show varying pigmentation patterns; some are similar to those of the type-specimens, while others show more extensive or intense head, antennae, legs and siphunculi pigmentation (Figure 1). In quantitative characters the new specimens are similar to those of type-specimens, although they extend the ranges of several features (Table 2); it does not appear that greater or lesser measurements or quantities are correlated with greater or lesser pigmentation. Alate viviparous females. Figure 2. Colour when alive bright emerald green, similar to apterous viviparae, with more-or-less dark brown head, antennae, most of legs and siphunculi. Body 2.88–3.88 mm long and pearshaped with long legs and antennae. In mounted specimens, abdomen pale in general with strong contrast to head and thorax, including antennae, rostrum and legs; these and the siphunculi are strongly pigmented, similar to those of the well-pigmented apterous viviparae. Alatae are also similar to apterae in many other qualitative characters (see Nieto Nafría et al. (2019) for apterous females) differing from them by having large and pigmented marginal sclerites on abdominal segments 1 to 4 that bear several setae, and small abdominal intersegmental muscular sclerites that are paler than the neighbouring segmental sclerites. Metric and meristic features in Table 2. Oviparous females. Figures 3A and 3C. Similar overall in both alive and mounted specimens to apterous viviparous females, and also in body length and pigmentation variation. Differing in having a more pigmented subgenital plate with more setae, and a slightly swollen proximal part of the hind tibiae bearing most of the scent plates, which are diverse in size and form. Metric and meristic data are in Table 2. Males. Figure 3B. Apterous. Brown-greenish when alive. They are also very similar to females, although slightly smaller, with shorter siphunculi and cauda; the cauda is more or less triangular. When mounted, pigmentation is also varied, although coxae, trochanters and cauda are darker than in females. In addition, setiferous sclerites on thoracic and abdominal segments are conspicuously larger and darker, marginal sclerites tend to coalesce between them, and there are dispersed ventral sclerites on abdominal segments 2 to 6 and a transverse band on segment 7. Quantitative features are in Table 2. ......continued on the next page Bionomics and geographical distribution. The new specimens do not change what is already known of the species with regard to its host plant, Adesmia boronioides, or geographical distribution, which is restricted to the eastern bank of Nahuel Huapi lake in the Argentinian province of Neuquén (Nieto Nafría et al. 2019). Discussion. The observed differences in pigmentation between specimens of all morphs can be explained as intraspecific variability independent of geography or host plant. The variation affects specimens from the same location and on the same plants, which were collected at the same or similar time of year (the new specimens were collected in mid-January and the type specimens were collected in mid-December). The great similarities that the specimens of this species —viviparous and oviparous females and males— present generally, in many qualitative details and most quantitative features, are highly remarkable. The apterism of the males and the small swelling of hind tibiae in oviparous females can be related to a monoecious, non-host-alternating, life cycle. The brevity of the life cycle of the species is also very remarkable; sexuales are present in the first summer month in a locality of very moderate altitude, which undoubtedly hinders collection of this species in other locations and impedes the knowledge of its distribution., Published as part of Durante, M. Pilar Mier, Ortego, Jaime, Von Dohlen, Carol D. & Nafría, Juan Manuel Nieto, 2020, A further contribution to the knowledge of Uroleucon species (Hemiptera, Aphididae) living on Adesmia (Fabaceae) in southern South America, with description of a new species from Chile, pp. 548-560 in Zootaxa 4748 (3) on pages 550-553, DOI: 10.11646/zootaxa.4748.3.8, http://zenodo.org/record/3701139, {"references":["Nieto Nafria, J. M., von Dohlen, C. D., Moreno-Gonzalez, V., Ortego, J. & Mier Durante, M. P. (2019) The species of Uroleucon (Hemiptera: Aphididae) living on Adesmia (Fabaceae) in Argentina, with description of a new species. Zootaxa, 4555 (4), 561 - 572. https: // doi. org / 10.11646 / zootaxa. 4555.4.7"]}

Uroleucon nahuelhuapense Nieto Nafría & von Dohlen, sp. n. Types. Holotype: apterous viviparous female, ARGENTINA, Neuquén province, Villa La Angostura: Santa María (road 231, km. 32), 40° 55 'S 71° 26’ W, 800 m; on Adesmia boronioides; December 14, 2009; Mier Durante, Ortego and Nieto Nafría leg., sample ARG 1670, specimen number 1; collection of the University of León (León, Spain). Paratypes: 32 viviparous apterous females of the same sample, collected at the same time as the holotype; collections of the University of León (León, Spain), Jaime Ortego (Mendoza, Argentina) and The Natural History Museum (London, United Kingdom). Apterous viviparous females. Figure 1. Based on 33 specimens. Colour when alive bright emerald green with brownish-green siphunculi. Body 2.90-3.53 mm long and pear-shaped with long legs and antennae. Mounted specimens more-or-less light brown with darkened head, rostrum, anal plate, cauda and usually antennae and legs, and brown to dark-brown siphunculi. Setae on dorsum of body, antennae and legs (except those on tarsi and end of tibiae) thick and with apex indistinct or truncate; other setae more-or-less pointed. Marginal tubercles absent. Several specimens have one or two very minute cephalic spinal tubercles. Frons sinuous, medial-frontal tubercle lower than the frontal-lateral (or antennal) tubercles. Cephalic setae with the more usual pattern in the South American species of the genus: behind the four aligned frontal setae there are from front to back one pair (anterior discal), one pair (posterior discal) and a transverse postero-cephalic line with four setae. Antennal segments I and II similar in colour to head or in part darker than it, especially around the ventro-medial muscular impressions of segment I and near the end of segment II; antennal segment III smooth as in the proximal ones, with a short proximal part pale brown as in the proximal segments and progressively darkened to a dark brown or near-black apical ring; remainder of antenna progressively darkened and imbricated. Sensoria round and with thick walls; primary sensoria ciliate; secondary sensoria small, non-protruding and aligned, with the most proximal one at the end of the paler part of the segment. Rostrum surpassing middle coxae and darkening to apex. Ultimate rostral segment relatively narrow. Coxae and trochanters more-or-less pigmented than head and most of femora, which present a more-or-less extensive dorso-apical area darker than the remaining segments. Tibiae somewhat darker than the femora and with 1/7-1/8 of their length apical dark brown or almost black. Tarsi short and as dark as the distal end of tibiae and second segment with ventral setae. Setiferous sclerites on thorax and on pre-siphuncular abdominal segments small or very small, mostly unpigmented, near inconspicuous. Pre- and post-siphuncular sclerites absent. Intersegmental muscular sclerites absent. Spiracular sclerites small and light brown. Siphunculi progressively pigmented, brown to dark brown, cylindrical with enlarged proximal part, in most of the length a little wider than hind tibiae; transverse striae in the paler anterior half, scales progressively more marked on the distal portion prior to reticulation, which is ill defined; flange little marked. Setiferous sclerites on abdominal segments 6 to 8 and more-or-less light pigmented; those on VIII segment can be partially fused, without forming a continuous transverse sclerite. Subgenital plate pale, not darker than the ventral part of anterior abdominal segment and paler than anal plate and cauda, which are scarcely pigmented. Cauda lanceolate, with a marked proximal narrowing and more-or-less blunt at the apex; its setae are all similar in length and longer than the caudal width at the middle. Metric and meristic features in Table 2. Alate viviparous females and sexuales. Unknown. Etymology. The specific name nahuelhuapense is a supposed demonym of Nahuel Huapi lake, in neutral gender because Uroleucon is neutral. Bionomics. Aphids feed on the small stems of Adesmia boronioides Hook. f. (Fabaceae), which is its only known host plant. Sexuales are unknown, but the species is likely to be holocyclic, because of cold temperatures during winter. Geographical distribution. The new species is currently known in one locality of the Andean slopes of the Argentinean province of Neuquén, very close to the shore of the Nahuel Huapi lake. It is possible that the species occurs on the same plant species or on other species of Adesmia in other localities of similar altitude in Argentina or even in Chile. Molecular analyses. Amplification and sequencing of the tRNAleu-COII locus were performed successfully and resulted in divergent sequences for all South American taxa. Sequences obtained were deposited in GenBank (Table 1). Taxonomic discussion. M.P. Mier and J. Ortego had some difficulty in 2008 convincing reviewers of the manuscript describing U. adesmiae that the new species should be classified as Uroleucon, because its host plant was in Fabaceae, not Asteraceae or Campanulaceae, the usual host-plant families of Uroleucon (Mier Durante & Ortego, 2008). Furthermore, several morphological features of U. adesmiae were not typical of Uroleucon (almost total absence of setiferous sclerites as well as pre- and post-siphuncular sclerites). Justification of U. nahuelhuapense sp. n. as a Uroleucon is simpler because there is a great resemblance to U. adesmiae and U. payuniense Ortego & Nieto Nafría 2007 (see Mier Durante & Ortego, 2008), and because it shares the genus of host plant with U. adesmiae. This classification is further justified by genetic evidence. The tRNAleu-COII mitochondrial sequences confirm the correct placement of U. nahuelhuapense sp. n. (as well as U. adesmiae) within Uroleucon (Figure 2). Both species living on Adesmia form a cluster with other South American native Uroleucon and one Euro-Asiatic species, U. rapunculoidis (Börner, 1939). While U. nahuelhuapense sp. n. and U. adesmiae are clustered most closely to each other, the magnitudes of their pairwise patristic distance (sum of branch lengths on the dendrogram) and Tamura-Nei distance are as great or greater than the distances between other established, valid species in the comparison (Table 3). Thus, these two taxa exhibit species-level genetic distances from each other and from other described Uroleucon, both South American and Northern Hemisphere species. It is generally accepted that the subgeneric classification of Uroleucon requires a thorough revision (see Blackman & Eastop, 2018). Our phylogenetic studies of Uroleucon in progress suggest that the South American species are not a monophyletic group, and that the subgenera as currently understood are not monophyletic (see also Moran et al., 1999). Until the necessary revision can be undertaken, however, it seems appropriate to place Uroleucon nahuelhuapense sp. n. in the subgenus Lambersius Olive, 1965, according to the concept of that subgenus in Nieto Nafría et al. (2007). Uroleucon nahuelhuapense sp. n. and U. adesmiae, the two species of Uroleucon living on Adesmia species, can be morphologically distinguished from one another by antennal pigmentation (darker in U. nahuelhuapense sp. n. than in U. adesmiae) and by different numbers of setae on the cephalic dorsum, on the ultimate rostral segment, on both dorsum and venter of abdominal presiphuncular segments, on genital plate and on cauda (see Table 2). Thirty species of Uroleucon have been recorded from South America: U. adesmiae Mier Durante & Ortego, 2008, U. aeneum (Hille Ris Lambers, 1939), U. ambrosiae (Thomas, 1878), U. bereticum (Blanchard, 1922), U. brevisiphon de Carvalho, 1998, U. chilense (Essig, 1953), U. compositae (Theobald, 1915), U. erigeronense (Thomas, 1878), U. essigi de Carvalho, 1998, U. eumadiae Delfino & Gonzales, 2005, U. garnicai Delfino, 1991, U. gochnatiae Delfino, 1994, U. gravicorne (Patch, 1919), U. jaceae (Linnaeus, 1758), U. littorale (Blanchard, 1939), U. macolai (Blanchard, 1932), U. malarguense Ortego & Nieto Nafría, 2007, U. mendocinum Mier Durante & Ortego, 2007, U. muermosum (Essig, 1953), U. nahuelhuapense sp.n., U. nuble (Essig, 1953), U. patagonicum Nieto Nafría & Seco Fernández, 2007, U. payuniense Ortego & Nieto Nafría, 2007, U. petrohuense de Carvalho, 1998, U. pseudomuermosum de Carvalho, 1998, U. riojanum Nieto Nafría & Mier Durante, 2007, U. rudbeckiae (Fitch, 1851), U. sonchi (Linnaeus, 1767), U. tessariae Delfino, 1994 and U. tucumani (Essig, 1953). Four of them: U. aeneum, U. compositae, U. jaceae and U. sonchi are species wide-distributed and not native to the Americas, they can be easily separated to the others 26 species, which are South American or North American native species, because they have coxae wholly dark brown to black, as pigmented or nearly as pigmented as distal apices of femora, tibiae and most part of siphunculi, which are dark-brown to black sometimes with paler middle portion. In other 26 species coxae are yellowish brown to brown, less pigmented than distal part of siphunculi and usually less pigmented than the distal area of femora, and siphunculi are variably pigmented but if the middle part is not as dark as proximal and distal part then cauda is triangular shaped, not lanceolate. Most part of these 26 species are restricted to South America, only U. ambrosiae (Thomas, 1878), U. erigeronense (Thomas, 1878), U. gravicorne (Patch, 1919) and U. rudbeckiae (Fitch, 1851) are also known in North America. parentheses are exceptional data, or very exceptional if parentheses are duplicates. Bold characters in the Uroleucon adesmiae column indicate that the previously known corresponding limit has been modified with the new data. Arrows, single or double, indicate the most important characteristics to differentiate both South American species of Uroleucon living on Adesmia. ......continued on the next page The following key permits the separation of the Uroleucon species with relatively pale coxae, as it has been detailed before, recorded from South America. It is based on that by Nieto Nafría et al. (2007), which in turn was based on the key by de Carvalho et al. (1998); several disjunctive couplets have been simplified. Uroleucon littorale was described from a locality in the Argentine province of Entre Ríos, it has not yet been collected and its original description is not too eloquent; it is very similar to U. tucumani, of which it can be synonymous in opinion by Blackman & Eastop (2018); it is not included in the key. The measurements are lengths unless otherwise indicated. In brackets are morphological data which do not have correspondence in the other proposition of the disjunctive, but which are useful to secure identification. Information on the distribution of each species has been actualized from our bibliographic registers. It includes countries for South America, regions for Chile and provinces for Argentina. Records from the Federal Capital of Argentina, Ciudad Autónoma de Buenos Aires, are joined to records from the Buenos Aires province. The names of Chilean regions Aysén del General Carlos Ibáñez del Campo, Libertador General Bernardo O’Higgins and Metropolitana de Santiago are simplified respectively to Aysén, O’Higgins and Santiago. 1 Ventral hairs on proximal 2/3 of hind tarsi second segment small or atrophied. [Siphunculus 1.0–1.2 times cauda. Marginal tubercles present on abdominal segment 2 to 5. Alatae with protuberant secondary sensoria on antennal segment III. Green in life]. On composite species, mainly of genera Erigeron and Solidago. Probably Neartic in origin; Brazil, Colombia, Venezuela......................................................................................... U. gravicorne - Ventral hairs on proximal 2/3 of hind tarsi second segment similar in shape and length to others ventral hairs on this segment and on other tarsi.................................................................................... 2 2 Antennal segment VI processus terminalis longer than 1.2 mm. Siphunculi at least 2.3 times cauda, thin, brown with a pale proximal portion, and reticulated at most on 15% of total length. [Probably pale green with brown o brownish head and partially appendages and siphunculi when alive.] On Senecio smithii, Symphyotrichum squamatus and perhaps other composite. Chile: Aysén, Ñuble.............................................................................. U. nuble - Antennal segment VI processus terminalis shorter than 1.00 mm. Siphunculi shorter or longer than 2.3 times cauda, but if longer it has a different appearance or pigmentation or reticulation of those mentioned above........................ 3 3 Posterior part of cephalic dorsum (behind the discal two setae of vertex) with 6–11 setae............................. 4 - Posterior part of cephalic dorsum (behind the discal two setae of vertex) usually with 4 setae (the general feature in the genus)................................................................................................... 6 4 Cauda triangular or long-pentagonal and 0.22‾ 0.30 mm. Abdominal segment 2 to 6 with 1‾2 marginal setae each side [and 5‾13 spinal and pleural setae]. Red with siphunculi dark brown when alive. [Siphunculi brown to dark brown in general often with a less pigmented middle portion]. On Mutisia spinosa. Argentina: Chubut and Neuquén............. U. patagonicum - Cauda lanceolate and 0.27–0.40 mm. Abdominal segments 2 to 4 with 3 or more marginal setae each side. Green or brownish green in life......................................................................................... 5 5 Siphunculi yellowish or very light-brown with paler proximal 1/4. Abdominal segments 2 to 6 with 6‾12 marginal setae each side and 16–27 setae on spinal and pleural areas. Antennal segment VI processus terminalis at least 1.0 times antennal segment III. Siphuncular width at the base 2.4–4.3 times its width at the beginning of the reticulation, which is well sculptured and extends over 12.2–18.9% of its length. Ultimate rostral segment with 7–10 accessory setae. Cauda with 6–9 setae. Dorsoabdominal setae 50–60 µm. Green in life. On Grindelia chiloensis. Argentina: Mendoza................... U. payuniense - Siphunculi homogeneous brown. Abdominal segments 2 to 6 with 3–6 marginal setae each side and 7–20 setae on spinal and pleural areas. Antennal segment VI processus terminalis at most 1.1 times antennal segment III. Siphuncular width at the base 1.6–2.9 times its width at the beginning of the reticulation, which is poorly sculptured and extends over only 8.0–13.4% its length, exceptionally to 14.3%. Ultimate rostral segment with 10–15 accessory setae. Cauda with 10–15 setae. Dorsoabdominal setae 35–45 µm. Dark green to brownish green in life. On Adesmia. Argentina: Chubut and Neuquén...... U. adesmiae 6 Dorso-cephalic setae 18‾36 µm. [Greenish yellow to yellowish pale green in life. Siphunculi with paler proximal portion, more evident in alatae than apterae. Antennal segment III with 4‾25 secondary sensoria on proximal 1/ 2 in apterae and 20‾41 secondary sensoria in alatae]. On species of several composite genera, in South America Baccharis, Conyza, Erigeron, Gutierrezia, Haplopappus and Hysterionica. Nearctic introduced species; Brazil, Colombia, Peru, Venezuela; Argentina: Buenos Aires, Córdoba, Entre Ríos, Jujuy, Mendoza, Salta, San Luis, Santa Fe, Santiago del Estero; Chile: Maule, Santiago, Valparaíso...................................................................................... U. erigeronense - Distal setae on cauda variable in length, if shorter than other caudal setae then usually pointed. Dorso-cephalic setae 30‾75 µm.................................................................................................... 7 7 Ultimate rostral segment 0.21‾ 0.27 mm and 1.5‾1.8 times hind tarsi second segment.............................. 8 - Ultimate rostral segment 0.12‾ 0.22 mm and 0.8‾1.4 times hind tarsi second segment.............................. 9 8 Ultimate rostral segment 0.23‾ 0.27 mm, with 6‾10 accessory setae. Dark reddish to brown or black when alive. On Acrisione denticulata and perhaps on species of Senecio. Brazil; Chile: La Araucanía, Los Lagos................... U. muermosum - Ultimate rostral segment 0.21‾ 0.23 mm, with 18–24 accessory setae. Shiny green in life. On Madia chilensis and M. sativa. Chile: Santiago.............................................................................. U. eumadiae 9 Marginal tubercles protuberant, frequently on abdominal segment 5 and sometimes on abdominal segments 2 to 4. Ultimate rostral segment with 4‾5 accessory setae. [Apterae with 14‾26 secondary sensoria distributed on nearly whole length of antennal segment III.] Probably pale green in life. On unknown composite. Chile: Los Lagos.................. U. petrohuense - Marginal papillae absent on abdominal segment 5, if present on abdominal segments 2 to 4 then not protuberant. Ultimate rostral segment usually with 5 or more accessory setae......................................................... 10 10 Antennal segment VI processus terminalis at most 3.8 times antennal segment VI base. Siphunculi reticulated on at least 35% of their length and yellowish brown to light brown, like cauda, which is lanceolate. Dark-green with dark-brown head. On Baccharis, Gochnatia glutinosa, Gutierrezia, Hyaloceris cinerea and Senecio subulatus. Bolivia; Argentina: Catamarca, Jujuy, La Rioja, Mendoza, Salta, Tucumán............................................................... U. gochnatiae - Antennal segment VI processus terminalis usually at least 3.8 times antennal segment VI base; if 3.1‾3.9 times then siphunculi or cauda h

Uroleucon adesmiae Mier Durante and Ortego, 2008. Studied material. ARGENTINA: Chubut province, Estancia ���San Rom��n��� (30 km East from Esquel on road number 40), 42�� 59' S 71�� 06' W, 730 m; on Adesmia sp.; December 6, 2009: 6 apterous viviparous females. ARGENTINA: Neuqu��n province, La Bomba (km 2302 on road number 40), 39�� 36' S 70�� 22' W, 770 m; on Adesmia sp.; December 13, 2009: 1 apterous viviparous female. Collection of the University of Le��n (Le��n, Spain). Apterous viviparous females of both samples do not show differences in qualitative features, including colour when alive and pigmentation when mounted on slides, in comparison to the type specimens of the species (R��o Mayo [approximately 45�� 41��� S 70�� 16��� W, 510 m] (Argentina, Chubut), on Adesmia sp., December 13, 2004). However there are some differences in the maximum and minimum limits of many of the measured characters (Table 2). These differences can be explained because the measurements and quantities in the original description were based exclusively on the type specimens, which were most likely closely related to each other, because they had been collected on the same day and location and on plants that grew very close to each other. In contrast, the measurements determined here were taken from specimens collected in two localities distant from the type locality (Estancia ���San Rom��n��� at 280 km and La Bomba at 640 km from the type locality of R��o Mayo). It is possible that the intra-specific diversity is even greater because all the studied specimens are from the same month and it is possible that the apterous viviparous females might show inter-generational variability., Published as part of Nafr��a, Juan Manuel Nieto, Von Dohlen, Carol D., Moreno-Gonz��lez, V��ctor, Ortego, Jaime & Durante, M. Pilar Mier, 2019, The species of Uroleucon (Hemiptera: Aphididae) living on Adesmia (Fabaceae) in Argentina, with the description of a new species, pp. 561-572 in Zootaxa 4555 (4) on page 562, DOI: 10.11646/zootaxa.4555.4.7, http://zenodo.org/record/2585355, {"references":["Mier Durante, M. P. & Ortego, J. (2008) A new species of Uroleucon (Hemiptera: Aphididae) living on Adesmia (Fabaceae). Florida Entomologist, 91, 367 - 371. https: // doi. org / 10.1653 / 0015 - 4040 (2008) 91 [367: ANSOUH] 2.0. CO; 2"]}

Uroleucon nahuelhuapense Nieto Nafr��a & von Dohlen, sp. n. Types. Holotype: apterous viviparous female, ARGENTINA, Neuqu��n province, Villa La Angostura: Santa Mar��a (road 231, km. 32), 40�� 55 'S 71�� 26��� W, 800 m; on Adesmia boronioides; December 14, 2009; Mier Durante, Ortego and Nieto Nafr��a leg., sample ARG 1670, specimen number 1; collection of the University of Le��n (Le��n, Spain). Paratypes: 32 viviparous apterous females of the same sample, collected at the same time as the holotype; collections of the University of Le��n (Le��n, Spain), Jaime Ortego (Mendoza, Argentina) and The Natural History Museum (London, United Kingdom). Apterous viviparous females. Figure 1. Based on 33 specimens. Colour when alive bright emerald green with brownish-green siphunculi. Body 2.90-3.53 mm long and pear-shaped with long legs and antennae. Mounted specimens more-or-less light brown with darkened head, rostrum, anal plate, cauda and usually antennae and legs, and brown to dark-brown siphunculi. Setae on dorsum of body, antennae and legs (except those on tarsi and end of tibiae) thick and with apex indistinct or truncate; other setae more-or-less pointed. Marginal tubercles absent. Several specimens have one or two very minute cephalic spinal tubercles. Frons sinuous, medial-frontal tubercle lower than the frontal-lateral (or antennal) tubercles. Cephalic setae with the more usual pattern in the South American species of the genus: behind the four aligned frontal setae there are from front to back one pair (anterior discal), one pair (posterior discal) and a transverse postero-cephalic line with four setae. Antennal segments I and II similar in colour to head or in part darker than it, especially around the ventro-medial muscular impressions of segment I and near the end of segment II; antennal segment III smooth as in the proximal ones, with a short proximal part pale brown as in the proximal segments and progressively darkened to a dark brown or near-black apical ring; remainder of antenna progressively darkened and imbricated. Sensoria round and with thick walls; primary sensoria ciliate; secondary sensoria small, non-protruding and aligned, with the most proximal one at the end of the paler part of the segment. Rostrum surpassing middle coxae and darkening to apex. Ultimate rostral segment relatively narrow. Coxae and trochanters more-or-less pigmented than head and most of femora, which present a more-or-less extensive dorso-apical area darker than the remaining segments. Tibiae somewhat darker than the femora and with 1/7-1/8 of their length apical dark brown or almost black. Tarsi short and as dark as the distal end of tibiae and second segment with ventral setae. Setiferous sclerites on thorax and on pre-siphuncular abdominal segments small or very small, mostly unpigmented, near inconspicuous. Pre- and post-siphuncular sclerites absent. Intersegmental muscular sclerites absent. Spiracular sclerites small and light brown. Siphunculi progressively pigmented, brown to dark brown, cylindrical with enlarged proximal part, in most of the length a little wider than hind tibiae; transverse striae in the paler anterior half, scales progressively more marked on the distal portion prior to reticulation, which is ill defined; flange little marked. Setiferous sclerites on abdominal segments 6 to 8 and more-or-less light pigmented; those on VIII segment can be partially fused, without forming a continuous transverse sclerite. Subgenital plate pale, not darker than the ventral part of anterior abdominal segment and paler than anal plate and cauda, which are scarcely pigmented. Cauda lanceolate, with a marked proximal narrowing and more-or-less blunt at the apex; its setae are all similar in length and longer than the caudal width at the middle. Metric and meristic features in Table 2. Alate viviparous females and sexuales. Unknown. Etymology. The specific name nahuelhuapense is a supposed demonym of Nahuel Huapi lake, in neutral gender because Uroleucon is neutral. Bionomics. Aphids feed on the small stems of Adesmia boronioides Hook. f. (Fabaceae), which is its only known host plant. Sexuales are unknown, but the species is likely to be holocyclic, because of cold temperatures during winter. Geographical distribution. The new species is currently known in one locality of the Andean slopes of the Argentinean province of Neuqu��n, very close to the shore of the Nahuel Huapi lake. It is possible that the species occurs on the same plant species or on other species of Adesmia in other localities of similar altitude in Argentina or even in Chile. Molecular analyses. Amplification and sequencing of the tRNAleu-COII locus were performed successfully and resulted in divergent sequences for all South American taxa. Sequences obtained were deposited in GenBank (Table 1). Taxonomic discussion. M.P. Mier and J. Ortego had some difficulty in 2008 convincing reviewers of the manuscript describing U. adesmiae that the new species should be classified as Uroleucon, because its host plant was in Fabaceae, not Asteraceae or Campanulaceae, the usual host-plant families of Uroleucon (Mier Durante & Ortego, 2008). Furthermore, several morphological features of U. adesmiae were not typical of Uroleucon (almost total absence of setiferous sclerites as well as pre- and post-siphuncular sclerites). Justification of U. nahuelhuapense sp. n. as a Uroleucon is simpler because there is a great resemblance to U. adesmiae and U. payuniense Ortego & Nieto Nafr��a 2007 (see Mier Durante & Ortego, 2008), and because it shares the genus of host plant with U. adesmiae. This classification is further justified by genetic evidence. The tRNAleu-COII mitochondrial sequences confirm the correct placement of U. nahuelhuapense sp. n. (as well as U. adesmiae) within Uroleucon (Figure 2). Both species living on Adesmia form a cluster with other South American native Uroleucon and one Euro-Asiatic species, U. rapunculoidis (B��rner, 1939). While U. nahuelhuapense sp. n. and U. adesmiae are clustered most closely to each other, the magnitudes of their pairwise patristic distance (sum of branch lengths on the dendrogram) and Tamura-Nei distance are as great or greater than the distances between other established, valid species in the comparison (Table 3). Thus, these two taxa exhibit species-level genetic distances from each other and from other described Uroleucon, both South American and Northern Hemisphere species. It is generally accepted that the subgeneric classification of Uroleucon requires a thorough revision (see Blackman & Eastop, 2018). Our phylogenetic studies of Uroleucon in progress suggest that the South American species are not a monophyletic group, and that the subgenera as currently understood are not monophyletic (see also Moran et al., 1999). Until the necessary revision can be undertaken, however, it seems appropriate to place Uroleucon nahuelhuapense sp. n. in the subgenus Lambersius Olive, 1965, according to the concept of that subgenus in Nieto Nafr��a et al. (2007). Uroleucon nahuelhuapense sp. n. and U. adesmiae, the two species of Uroleucon living on Adesmia species, can be morphologically distinguished from one another by antennal pigmentation (darker in U. nahuelhuapense sp. n. than in U. adesmiae) and by different numbers of setae on the cephalic dorsum, on the ultimate rostral segment, on both dorsum and venter of abdominal presiphuncular segments, on genital plate and on cauda (see Table 2). Thirty species of Uroleucon have been recorded from South America: U. adesmiae Mier Durante & Ortego, 2008, U. aeneum (Hille Ris Lambers, 1939), U. ambrosiae (Thomas, 1878), U. bereticum (Blanchard, 1922), U. brevisiphon de Carvalho, 1998, U. chilense (Essig, 1953), U. compositae (Theobald, 1915), U. erigeronense (Thomas, 1878), U. essigi de Carvalho, 1998, U. eumadiae Delfino & Gonzales, 2005, U. garnicai Delfino, 1991, U. gochnatiae Delfino, 1994, U. gravicorne (Patch, 1919), U. jaceae (Linnaeus, 1758), U. littorale (Blanchard, 1939), U. macolai (Blanchard, 1932), U. malarguense Ortego & Nieto Nafr��a, 2007, U. mendocinum Mier Durante & Ortego, 2007, U. muermosum (Essig, 1953), U. nahuelhuapense sp.n., U. nuble (Essig, 1953), U. patagonicum Nieto Nafr��a & Seco Fern��ndez, 2007, U. payuniense Ortego & Nieto Nafr��a, 2007, U. petrohuense de Carvalho, 1998, U. pseudomuermosum de Carvalho, 1998, U. riojanum Nieto Nafr��a & Mier Durante, 2007, U. rudbeckiae (Fitch, 1851), U. sonchi (Linnaeus, 1767), U. tessariae Delfino, 1994 and U. tucumani (Essig, 1953). Four of them: U. aeneum, U. compositae, U. jaceae and U. sonchi are species wide-distributed and not native to the Americas, they can be easily separated to the others 26 species, which are South American or North American native species, because they have coxae wholly dark brown to black, as pigmented or nearly as pigmented as distal apices of femora, tibiae and most part of siphunculi, which are dark-brown to black sometimes with paler middle portion. In other 26 species coxae are yellowish brown to brown, less pigmented than distal part of siphunculi and usually less pigmented than the distal area of femora, and siphunculi are variably pigmented but if the middle part is not as dark as proximal and distal part then cauda is triangular shaped, not lanceolate. Most part of these 26 species are restricted to South America, only U. ambrosiae (Thomas, 1878), U. erigeronense (Thomas, 1878), U. gravicorne (Patch, 1919) and U. rudbeckiae (Fitch, 1851) are also known in North America. parentheses are exceptional data, or very exceptional if parentheses are duplicates. Bold characters in the Uroleucon adesmiae column indicate that the previously known corresponding limit has been modified with the new data. Arrows, single or double, indicate the most important characteristics to differentiate both South American species of Uroleucon living on Adesmia. ......continued on the next page The following key permits the separation of the Uroleucon species with relatively pale coxae, as it has been detailed before, recorded from South America. It is based on that by Nieto Nafr��a et al. (2007), which in turn was based on the key by de Carvalho et al. (1998); several disjunctive couplets have been simplified. Uroleucon littorale was described from a locality in the Argentine province of Entre R��os, it has not yet been collected and its original description is not too eloquent; it is very similar to U. tucumani, of which it can be synonymous in opinion by Blackman & Eastop (2018); it is not included in the key. The measurements are lengths unless otherwise indicated. In brackets are morphological data which do not have correspondence in the other proposition of the disjunctive, but which are useful to secure identification. Information on the distribution of each species has been actualized from our bibliographic registers. It includes countries for South America, regions for Chile and provinces for Argentina. Records from the Federal Capital of Argentina, Ciudad Aut��noma de Buenos Aires, are joined to records from the Buenos Aires province. The names of Chilean regions Ays��n del General Carlos Ib����ez del Campo, Libertador General Bernardo O���Higgins and Metropolitana de Santiago are simplified respectively to Ays��n, O���Higgins and Santiago. 1 Ventral hairs on proximal 2/3 of hind tarsi second segment small or atrophied. [Siphunculus 1.0���1.2 times cauda. Marginal tubercles present on abdominal segment 2 to 5. Alatae with protuberant secondary sensoria on antennal segment III. Green in life]. On composite species, mainly of genera Erigeron and Solidago. Probably Neartic in origin; Brazil, Colombia, Venezuela......................................................................................... U. gravicorne - Ventral hairs on proximal 2/3 of hind tarsi second segment similar in shape and length to others ventral hairs on this segment and on other tarsi.................................................................................... 2 2 Antennal segment VI processus terminalis longer than 1.2 mm. Siphunculi at least 2.3 times cauda, thin, brown with a pale proximal portion, and reticulated at most on 15% of total length. [Probably pale green with brown o brownish head and partially appendages and siphunculi when alive.] On Senecio smithii, Symphyotrichum squamatus and perhaps other composite. Chile: Ays��n, ��uble.............................................................................. U. nuble - Antennal segment VI processus terminalis shorter than 1.00 mm. Siphunculi shorter or longer than 2.3 times cauda, but if longer it has a different appearance or pigmentation or reticulation of those mentioned above........................ 3 3 Posterior part of cephalic dorsum (behind the discal two setae of vertex) with 6���11 setae............................. 4 - Posterior part of cephalic dorsum (behind the discal two setae of vertex) usually with 4 setae (the general feature in the genus)................................................................................................... 6 4 Cauda triangular or long-pentagonal and 0.22��� 0.30 mm. Abdominal segment 2 to 6 with 1���2 marginal setae each side [and 5���13 spinal and pleural setae]. Red with siphunculi dark brown when alive. [Siphunculi brown to dark brown in general often with a less pigmented middle portion]. On Mutisia spinosa. Argentina: Chubut and Neuqu��n............. U. patagonicum - Cauda lanceolate and 0.27���0.40 mm. Abdominal segments 2 to 4 with 3 or more marginal setae each side. Green or brownish green in life......................................................................................... 5 5 Siphunculi yellowish or very light-brown with paler proximal 1/4. Abdominal segments 2 to 6 with 6���12 marginal setae each side and 16���27 setae on spinal and pleural areas. Antennal segment VI processus terminalis at least 1.0 times antennal segment III. Siphuncular width at the base 2.4���4.3 times its width at the beginning of the reticulation, which is well sculptured and extends over 12.2���18.9% of its length. Ultimate rostral segment with 7���10 accessory setae. Cauda with 6���9 setae. Dorsoabdominal setae 50���60 ��m. Green in life. On Grindelia chiloensis. Argentina: Mendoza................... U. payuniense - Siphunculi homogeneous brown. Abdominal segments 2 to 6 with 3���6 marginal setae each side and 7���20 setae on spinal and pleural areas. Antennal segment VI processus terminalis at most 1.1 times antennal segment III. Siphuncular width at the base 1.6���2.9 times its width at the beginning of the reticulation, which is poorly sculptured and extends over only 8.0���13.4% its length, exceptionally to 14.3%. Ultimate rostral segment with 10���15 accessory setae. Cauda with 10���15 setae. Dorsoabdominal setae 35���45 ��m. Dark green to brownish green in life. On Adesmia. Argentina: Chubut and Neuqu��n...... U. adesmiae 6 Dorso-cephalic setae 18���36 ��m. [Greenish yellow to yellowish pale green in life. Siphunculi with paler proximal portion, more evident in alatae than apterae. Antennal segment III with 4���25 secondary sensoria on proximal 1/ 2 in apterae and 20���41 secondary sensoria in alatae]. On species of several composite genera, in South America Baccharis, Conyza, Erigeron, Gutierrezia, Haplopappus and Hysterionica. Nearctic introduced species; Brazil, Colombia, Peru, Venezuela; Argentina: Buenos Aires, C��rdoba, Entre R��os, Jujuy, Mendoza, Salta, San Luis, Santa Fe, Santiago del Estero; Chile: Maule, Santiago, Valpara��so...................................................................................... U. erigeronense - Distal setae on cauda variable in length, if shorter than other caudal setae then usually pointed. Dorso-cephalic setae 30���75 ��m.................................................................................................... 7 7 Ultimate rostral segment 0.21��� 0.27 mm and 1.5���1.8 times hind tarsi second segment.............................. 8 - Ultimate rostral segment 0.12��� 0.22 mm and 0.8���1.4 times hind tarsi second segment.............................. 9 8 Ultimate rostral segment 0.23��� 0.27 mm, with 6���10 accessory setae. Dark reddish to brown or black when alive. On Acrisione denticulata and perhaps on species of Senecio. Brazil; Chile: La Araucan��a, Los Lagos................... U. muermosum - Ultimate rostral segment 0.21��� 0.23 mm, with 18���24 accessory setae. Shiny green in life. On Madia chilensis and M. sativa. Chile: Santiago.............................................................................. U. eumadiae 9 Marginal tubercles protuberant, frequently on abdominal segment 5 and sometimes on abdominal segments 2 to 4. Ultimate rostral segment with 4���5 accessory setae. [Apterae with 14���26 secondary sensoria distributed on nearly whole length of antennal segment III.] Probably pale green in life. On unknown composite. Chile: Los Lagos.................. U. petrohuense - Marginal papillae absent on abdominal segment 5, if present on abdominal segments 2 to 4 then not protuberant. Ultimate rostral segment usually with 5 or more accessory setae......................................................... 10 10 Antennal segment VI processus terminalis at most 3.8 times antennal segment VI base. Siphunculi reticulated on at least 35% of their length and yellowish brown to light brown, like cauda, which is lanceolate. Dark-green with dark-brown head. On Baccharis, Gochnatia glutinosa, Gutierrezia, Hyaloceris cinerea and Senecio subulatus. Bolivia; Argentina: Catamarca, Jujuy, La Rioja, Mendoza, Salta, Tucum��n............................................................... U. gochnatiae - Antennal segment VI processus terminalis usually at least 3.8 times antennal segment VI base; if 3.1���3.9 times then siphunculi or cauda h, Published as part of Nafr��a, Juan Manuel Nieto, Von Dohlen, Carol D., Moreno-Gonz��lez, V��ctor, Ortego, Jaime & Durante, M. Pilar Mier, 2019, The species of Uroleucon (Hemiptera: Aphididae) living on Adesmia (Fabaceae) in Argentina, with the description of a new species, pp. 561-572 in Zootaxa 4555 (4) on pages 563-570, DOI: 10.11646/zootaxa.4555.4.7, http://zenodo.org/record/2585355, {"references":["Mier Durante, M. P. & Ortego, J. (2008) A new species of Uroleucon (Hemiptera: Aphididae) living on Adesmia (Fabaceae). Florida Entomologist, 91, 367 - 371. https: // doi. org / 10.1653 / 0015 - 4040 (2008) 91 [367: ANSOUH] 2.0. CO; 2","Nieto Nafria, J. M., Mier Durante, M. P., Ortego, J. & Seco Fernandez, M. V. (2007) The genus Uroleucon (Hemiptera: Aphididae: Macrosiphini) in Argentina, with descriptions of five new species. The Canadian Entomologist, 139, 154 - 178. https: // doi. org / 10.4039 / n 05 - 100","Blackman, R. L. & Eastop, V. F. (2018) Aphids on World's plants. An online identification and information guide. Available from: http: // www. aphidsonworldsplants. info (accessed 28 June 2018)","Moran, N. A., Kaplan, M. E., Gelsey, M. J., Murphy, T. G. & Scholes, E. A. (1999) Phylogenetics and evolution of the aphid genus Uroleucon based on mitochondrial and nuclear DNA sequences. Systematic Entomology, 24, 85 - 93. https: // doi. org / 10.1046 / j. 1365 - 3113.1999.00076. x"]}

A new Rhus gall aphid species Nurudea zhengii Ren, sp. nov. collected from the Mountain Qixing in Shangrao County, Jiangxi Province, China is described and illustrated from alate viviparous female. The new species differs from the other Nurudea species in the length and proportion of antennal segments, the structure of antennal secondary sensilla, and the flower-like shape of the galls that are formed on its primary host. Its primary host plant is Rhus hypoleuca, whereas other Nurudea species are on R. chinensis.

Ageniella (Cyrtagenia) fallax (Arlé, 1947) (FIg. 5) Ameragenia fallax ArLé, 1947, REv. dE ENt., vOL. 18, p. 426 [HOLOtypE: ♀, BRAZIL, NItEróI (MNRJ [?]), LOSt]. Distribution. ARgENTINa, BRaZIL, COLOmbIa, PaNama, PERU (CaSTRO-HUERTaS et al. 2014). Host. UNkNOWN. Remarks. IN ThE ORIgINaL pUbLIcaTION, ARLÉ (1947) aSSIgNS ThE hOLOTYpE TO ThE aUThOR’S cOLLEcTION, WhIch WaS MUSEU NacIONaL dO RIO dE JaNEIRO (MNRJ) aT ThE TImE. NEVERThELESS, ThE hOLOTYpE Of A. fallax (FIg. 5) IS NOT dEpOSITEd aT MNRJ. DR. ROgER ARLÉ aLSO WORkEd aT ThE MPEG, IN PaRÁ, BRaZIL, WhIch WaS VISITEd bY CW aNd cONfIRmEd ThaT ThE hOLOTYpE mIghT bE LOST, aLONg WITh OThER TYpES dEScRIbEd bY DR. ARLÉ. IN ThE fUTURE, a NEOTYpE maY NEEd TO bE dESIgNaTEd., Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on page 144, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065

Ageniella (Alasagenia) flavipennis (Banks, 1946), comb. nov. Lissagenia flavipennis BaNkS, 1946, MUS. COMp. ZOOL. BULL., vOL. 96, p. 457 [HOLOtypE: ♀, BRAZIL, SaNta CatarINa (MCZ #26779), ExaMINEd]. Distribution. BRaZIL. Host. Enoploctenus cyclothorax (BERTkaU) (ARaNEaE: CTENIdaE) (SaNTOS et al. 2017). Remarks. ThIS IS ThE TYpE SpEcIES fOR Lissagenia. EVaNS (1973) dIffERENTIaTEd A. (Alasagenia) fROm A. (Lissagenia) bY ThE pRESENcE Of SERRaTE TIbIaE—WhEN VIEWEd IN pROfILE—IN ThE fORmER gROUp. HOWEVER, maLES Of A. (Alasagenia) Lack SpINES IN ThE hINd TIbIa aS WELL. WE jUSTIfY ThE NEW cOmbINaTION, aNd cONSEQUENTLY ThE NEW SUbgENUS SYNONYm, bY A. flavipennis haVINg SpEcImENS SETOSE aNd LaRgE, ThE cLYpEUS IS LaRgE aNd TRapEZOIdaL, ThE mESOpLEURUm IS pROjEcTEd LaTERaLLY IN fEmaLES, aNd ThE maLE SpEcImENS Lack STRONg SpINES ON ThE dORSaL facE Of ThE hINd TIbIa. ALL ThESE chaRacTERS aRE dIagNOSTIc fOR SUbgENUS Alasagenia. SaNTOS et al. (2017) REpORTEd fEmaLES Of A. flavipennis NESTINg cOmmUNaLLY IN BRaZIL, SãO PaULO STaTE, bY USINg mUd NESTS. ThIS WaS ThE fIRST REcORd Of a SpEcIES Of Ageniella bOTh NESTINg cOmmUNaLLY aNd USINg pRObabLY pRE-ExISTINg mUd NESTS.

Ageniella (Ameragenia) similaris (Banks, 1946) Ameragenia similaris BaNkS, 1946, MUS. COMp. ZOOL. BULL., vOL. 96, p. 433 [HOLOtypE: ♀, BRAZIL, SãO PaULO (MCZ #26764), ExaMINEd]. Distribution. BRaZIL. Host. UNkNOWN. Remarks. ThE bOdY-ShapE IS SOmEWhaT SImILaR TO ThE NEaRcTIc SpEcIES A. arcuata (BaNkS). Ageniella similaris, hOWEVER, LackS ThE ROW Of ScaLE-LIkE SpINES SEEN IN A. arcuata aNd pRESENT IN aLL NEaRcTIc fEmaLES Of Ageniella (Priophanes) (A. similaris haS TWO ROWS Of SpINES IN ThE hINd TIbIa, bUT ThEY aRE NOT ScaLE-LIkE). MOREOVER, ThE bOdY IS cOVEREd bY SILVER SETaE. BaNkS (1946) mORphOLOgIcaLLY RELaTEd ThIS SpEcIES TO A. (Priophanes) rufigaster (BaNkS), bUT dIffERENTIaTE ThEm fROm A. similaris bY ThE LaTER haVINg LEgS mOSTLY bLack, ThE hEad IS mORE SETacEOUS, ThE STIgma IS SLENdER aNd paLER, aNd ThE INNER SpUR IS ShORTER IN A. similaris ThaN IN A. rufigaster. BESIdES, BaNkS (1946) STaTEd ThaT ThE cELL 3RS IS LONgER IN A. similaris. ThE ORIgINaL dEScRIpTION IS ThE ONLY REcORd Of ThIS SpEcIES., Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on page 143, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065

Ageniella (Priophanes) rufitarsis (Fox, 1897), comb. nov. (FIg. 12) Salius rufitarsis FOx, 1897, Acad. Nat. ScI. PhILa. PrOc., vOL. 49, p. 268 [HOLOtypE: ♀, BRAZIL, Chapada (CMNH), ExaMINEd]. Distribution. BRaZIL. Host. UNkNOWN. Remarks. SamE SITUaTION aS ThE pREVIOUS SpEcIES dEScRIbEd fOR A. citricornis (FOx) (SEE REmaRkS). ThE SpEcIES haS ThE fORE TIbIa, INcLUdINg ThE VERY dISTaL pORTION Of ThE fORE fEmUR, mId aNd hINd TaRSI ORaNgE-RUfOUS; ThE apIcaL aNd SUbapIcaL TaRSI Of ThE mId LEg aRE bROWN; aNd ThE aNTENNaE haVE ThE SIx fIRST SEgmENTS ORaNgE-RUfOUS, ThE SEVENTh SEgmENT haS bOTh, ORaNgE aNd bROWN cOLORS, WhEREaS ThE REmaININg aNTENNaL SEgmENTS aRE bROWN., Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on page 149, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065

Key to the species of Ameragenia from Brazil (females) 1 Forewing maculated with dark bands or distinct dark spots..................................................... 2 - Forewing without maculations of dark bands or dark spots, if dark spot present, then very weak...................... 10 2 Integument black and rufous............................................................................. 3 - Integument entirely black............................................................................... 5 3 Clypeus large, apical margin with two median teeth; head and pronotum rufous; metasoma black with blue metallic reflections; forewing hyaline with two dark bands.................................................. A. sanguinolenta (Smith) - Clypeus various; head black (clypeus rufous in A. fabricii), pronotum various; metasoma various; forewing with or without dark bands........................................................................................... 4 4 Metasoma entirely rufous; mesosoma and head black; forewings with two dark bands, the one on the basal vein narrow.......................................................................................... A. similaris (Banks) - Metasoma black; mesosoma entirely or partially rufous, head black, clypeus rufous; forewing with two dark bands, the most apical incomplete........................................................................ A. fabricii (Banks) 5 Forewing with dark spots partially covering cell 2M and fully covering cell 2Rs; cell 2Rs 0.69 X as long as 3 Rs; clypeus trap- ezoidal, slightly arched; fore tibia pale brown................................................ A. agitata (Smith) - Forewing with one or two transversal dark bands; cells 2Rs and 3Rs different size; clypeus various; fore tibia black........ 6 6 Forewing with only one distinct dark band (sometime inconspicuous); silver setae covering integument on face and distal por- tion of propodeum..................................................................................... 7 - Forewing with two distinct dark bands..................................................................... 8 7 Mid and hind femur, and inner side of fore femur pale brown; absence of blue reflections on the hindwing venation and integu- ment................................................................................ A. rustica (Fabricius) - Fore, mid and hind femur black; blue reflections on hindwing venation and integument................. A. zeteki (Banks) 8 Integument black without golden pubescence; antennae yellow or at least some segments yellow..................... 9 - Integument black with golden pubescence, except propodeum, which has silver pubescence; antennal segments all black.......................................................................................... A. banksii (Banks) 9 Clypeus black; antennal segments 7–10 yellowish............................................. A. citricornis (Fox) - Clypeus pale brown; antennal segments entirely yellow........................................ A. pretiosa (Banks) 10 Integument black with fore, mid and hind tibia pale brown; forewing with a faint dark spot on cells 2Rs and 2M........ 11 - Integument black, tibia black; forewing various............................................................ 12 11 Integument with faint bluish reflections on head and metasoma; frons covered with silver scale-like setae from torulus to clypeus; clypeus trapezoidal................................................................ A. cleora (Banks) - Integument without bluish reflections on head and metasoma; frons without silver scale-like setae; clypeus rounded............................................................................................... A. varipes (Fox) 12 Clypeus large, arched, anterior margin angulate medially......................................... A. clypeata (Fox) - Clypeus truncate..................................................................................... 13 13 Legs and antennae entirely black; cell 2Rs about 0.65 X as long as 3Rs, tCu2 vein curved, very arched, as well as vein tCu1......................................................................................... A. thione (Banks) - Legs and antennae black, tibia with yellowish coloration; cells 2Rs and 3Rs almost equal size............. A. serrula (Fox), Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on pages 138-139, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065

Ageniella (Priophanes) cingulata (Fox, 1897), comb. nov. (FIgS. 8, 9) Agenia cingulata FOx, 1897, Acad. Nat. ScI. PhILa. PrOc., vOL. 49, p. 243–244 [HOLOtypE: ♂, BRAZIL, Chapada (CMNH), ExaMINEd]. Distribution. BRaZIL. Host. UNkNOWN. Remarks. ThE SpEcImEN WaS STUdIEd aT ThE CaRNEgIE COLLEcTION aNd had a LabEL addEd bY DREISbach aNd daTEd fROm 1961, WhIch IdENTIfIEd IT aS Priophanes nigerrima (FOx). TO OUR kNOWLEdgE, NO fORmaL SYNONYm WaS madE aNd WE abSTaIN fROm makINg ThE SYNONYm fOR Lack Of jUSTIfIcaTION. Ageniella nigerrima (FIgS. 10, 11) IS kNOWN fROm fEmaLES, WhEREaS A. cingulata (FIg. 8) IS kNOWN fROm maLES. ThIS SpEcImEN IS aNOThER SpEcIES fOUNd amONg FOx’S TYpES IN ThE CaRNEgIE COLLEcTION (SEE A. (Ameragenia) citricornis (FOx) REmaRkS).

Ageniella (Ameragenia) agitata (Smith, 1873), comb. nov. Agenia agitata SMIth, 1873, ANN. Mag. Nat. HISt., vOL. (4)11, p. 445 [HOLOtypE: ♀, BRAZIL, Pará: SaNtaréM (BMNH), ExaMINEd]. Salius (Priocnemis) setaceicornis FOx, 1897, Acad. Nat. ScI. PhILa. PrOc., vOL. 49, p. 269 [HOLOtypE: ♀, BRAZIL, [Pará], SaNtaréM (CMNH), ExaMINEd], syn. nov. Distribution. BRaZIL. Host. UNkNOWN. Remarks. Ageniella agitata IS RELaTEd TO A. plagosa (BaNkS) baSEd ON SImILaRITIES IN mORphOLOgY. ThEY dIffER IN fOREWINg VENaTION aNd cLYpEaL ShapE. Ageniella agitata haS ThE 2 RS cELL aLmOST aS LONg aS WIdE aNd WITh ThE EdgES aNgULaTE, WhEREaS A. plagosa haS 2 RS LONgER ThaN WIdE WITh ThE RS VEIN cURVEd. ThE cLYpEUS IN A. agitata IS SOmEWhaT aRchEd, WITh ThE apIcaL maRgIN ROUNdEd aNd cOVEREd WITh SILVER ScaLE-LIkE SETaE; A. plagosa haS ThE cLYpEUS TRapEZOIdaL WITh ThE apIcaL maRgIN STRaIghT aNd LackINg SILVER SETaE ON IT. WE jUSTIfY SYNONYmIZINg Salius setaceicornis FOx WITh A. agitata baSEd ON ThE fOREWINg VENaTION dEScRIbEd abOVE. MOREOVER, ThE fOREWINg haS INfUScaTE SpOTS paRTIaLLY cOVERINg ThE cELL 2M aNd fULLY cOVERINg ThE cELL 2 RS; ThE hEad aNd ThE cLYpEUS Of S. setaceicornis IS aRchEd WITh ThE apIcaL maRgIN ROUNdEd; ThE cLYpEUS aNd ThE pORTION RIghT abOVE IT aRE cOVEREd bY SILVER ScaLE-LIkE SETaE, aS IN A. agitata.

Ageniella (Ameragenia) gracilenta (Smith, 1873) Agenia gracilenta SMIth, 1873, ANN. Mag. Nat. HISt., vOL. (4)11, p. 448 [HOLOtypE: ♂, BRAZIL, Pará (BMNH), NOt ExaMINEd]. Distribution. BRaZIL. Host. UNkNOWN. Remarks. ThE SpEcIES WaS baSEd ON a maLE SpEcImEN fROm PaRÁ STaTE. PLacEmENT IN Ameragenia IS UNcERTaIN aNd IT IS pRObabLY a maLE Of a kNOWN fEmaLE. BEcaUSE ThE TYpE WaS NOT ExamINEd, WE abSTaIN fROm pROpOSINg SYNONYm Of ThIS SpEcIES., Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on pages 140-141, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065

Ageniella (Alasagenia) erichsoni (Banks, 1944) (FIg. 1) Alasagenia erichsoni BaNkS, 1944, ZOOLOgIca, vOL. 29, p. 107 [HOLOtypE: ♀, BRITISH GUIANA, KatarbO (MCZ #26180), ExaMINEd]. Priophanes major BaNkS, 1945, BOL. ENt. VENEz., vOL. 4, p. 122 [HOLOtypE: ♀, TRINIDAD, POrt Of SpaIN (CUIC), ExaMINEd], syn. nov. Distribution. BRaZIL (NEW REcORd), TRINIdad aNd TObagO, GUIaNa (FERNÁNdEZ 2000). Examined material. BRAZIL: MA, 1 ♀, ImpERaTRIZ, BaNaNaL, ARmadILha SUSpENSa 1.6 m, 19–21.VII.1989, F.F. Ramos coll. (MPEG-HYM #1109904663); 1 ♀ Alto Turlaçu, Aldeia Gurupluna, Armadilha suspensa 1.6 m, 26.ii– 02.III.1990, HELdER QUEIROZ cOLL. (MPEG-HYM #11090462). Host. UNkNOWN. Remarks. WE ExpaNd ThE RaNgE Of ThE SpEcIES TO NORThEaSTERN Of BRaZIL. SpEcIES Of A. erichsoni (FIg. 1) aRE dISTINgUIShEd bY haVINg a LaRgE bOdY, faIRLY SETOSE INTEgUmENT, aNd baNdEd fOREWINg. WE jUSTIfY SYNONYmIZINg A. major WITh A. erichsoni baSEd ON ThE fOLLOWINg ShaREd chaRacTERS: bLack INTEgUmENT; pRESENcE Of LONg-abUNdaNT SILVER pUbEScENcE ON ThE INTEgUmENT, WITh SpOTS Of ScaLE-LIkE SILVER pUbEScENcE ON ThE facE, mESOpLEURON, pROpOdEUm, aNd mETaSOma; LaRgE, TRapEZOIdaL cLYpEUS; SERRaTE hINd TIbIa; pRESENcE Of TWO daRk baNdS, ONE paRTIaLLY cOVERINg cELLS R, 1M, 1CU–2CU, aNd A (LESS daRk IN ThE LaST cELL), ThE OThER baNd cOVERS ThE cELLS 2R 1,1RS–2RS, aNd 2M; aNd ThE apEx Of fOREWINg IS daRkENEd., Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on pages 137-138, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065

Ageniella (Ameragenia) clypeata (Fox, 1897), comb. nov. Salius clypeatus FOx, 1897, Acad. Nat. ScI. PhILa. PrOc., vOL. 49, p. 270 [HOLOtypE: ♀, BRAZIL, [Pará: SaNtaréM] (CMNH), ExaMINEd]. Distribution. BRaZIL. Host. UNkNOWN. Remarks. SamE SITUaTION aS pREVIOUSLY dEScRIbEd fOR A. citricornis (FOx) (SEE REmaRkS).

Ageniella (Ameragenia) fragilis (Fox, 1897) Agenia fragilis FOx, 1897, Acad. Nat. ScI. PhILa. PrOc., vOL. 49, p. 243–244 [HOLOtypE: ♂, [BRAZIL, Pará]: SaNtaréM (CMNH), ExaMINEd]. Distribution. BRaZIL, HONdURaS, COLOmbIa (FERNÁNdEZ 2000; WaIchERT et al. 2014). Host. UNkNOWN. Remarks. ThE SpEcIES WaS dEScRIbEd baSEd ON a SINgLE maLE SpEcImEN. ThIS maLE RESEmbLES SpEcImENS Of Auplopus SpINOLa aNd Priocnemis SchIødTE bY haVINg ThE cLYpEUS WhITISh aNd ROUNdEd aNd ThE fIRST mETaSOmaL TERgUm WIdE, NOT aS pETIOLaTE aS SEEN IN maLES Of Ageniella., Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on page 141, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065

Ageniella (Priophanes) pallicornis (Banks, 1946) Priophanes pallicornis BaNkS, 1946, MUS. COMp. ZOOL. BULL., vOL. 96, p. 445 [HOLOtypE: ♀, BRAZIL, SãO PaULO (CUIC), ExaMINEd]. Distribution. BRaZIL. Host. UNkNOWN. Remarks. ThIS SpEcIES IS mORphOLOgIcaLLY RELaTEd TO maNY OThER SpEcIES ThaT OccUR IN BRaZIL SUch aS A. dolorosa (BaNkS). ThEY aLL haVE ThE INTEgUmENT bLack WITh bLUISh mETaLLIc REfLEcTIONS. ThE fOREWINg, hOWEVER, IS ThE maIN dISTINcTION. Ageniella pallicornis haS TWO daRk baNdS; ThE fIRST baNd paRTIaLLY cOVERS VEINS R, 1CU, 2CU, aNd 1M, ThE OThER paRTIaLLY cOVERS cELLS 2R1, 1RS, 2M aNd 1M, aNd fULLY cOVERS 2RS; WhEREaS A. dolorosa haS hYaLINE WINg WITh a daRk SpOT ON 2RS aNd 2M. MOREOVER, A. pallicornis haS paLE bROWN fORE aNd hINd LEgS, dIffERINg fROm A. rufitarsis (FIgS. 11, 12) ThaT haS ThE fIRST SEVEN aNTENNaL SEgmENTS aNd aLL TaRSI ORaNgE-RUfOUS., Published as part of Waichert, Cecilia, Colombo, Wesley Dondoni, Von Dohlen, Carol D. & Pitts, James P., 2018, Taxonomic contributions to Ageniella Banks, 1912 (Hymenoptera: Pompilidae) from Brazil, pp. 133-153 in Zootaxa 4403 (1) on page 149, DOI: 10.11646/zootaxa.4403.1.8, http://zenodo.org/record/1212065