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8 results on '"Dobiasova, S."'

2. Candida krusei, a multidrug-resistant opportunistic fungal pathogen: geographic and temporal trends from the ARTEMIS DISK Antifungal Surveillance Program, 2001 to 2005

Author

Pfaller, M.A, Diekema, D.J, Gibbs, D.L, Newell, V.A, Nagy, E, Dobiasova, S, Rinaldi, M, Barton, R, Veselov, A, Finquelievich, J, Tiraboschi, N, Ellis, D, Fameree, D, van den Abeele, A.-M, Senterre, J.-M, Lopez Colombo, A, Rennie, R, Sanche, S, Bijie, H, Xu, Y, Fu, W, Zhong, N.S, Rivas, P, de Bedout, C, Mendez, M, Vega, R, Mallatova, N, Ayabaca, J, Zurita, J, Mallie, M, Candolfi, E, Fegeler, W, Haase, P.D.G, Rodloff, A, Bar, W, Czaika, V, Petrikos, G, Puskás, E, Dóczi, I, Mestyan, G, Nikolova, R, Banerjee, U, Keller, N, Tullio, V, Carlo Schito, G, D'Antonio, D, Martino, P, Peng, N.K, Alpuche, C, Santos, J, Morfin Ortero, R, Zaidi, M, Meis, J.F, Lingaas, E, Dzierzanowska, D, Pawliszyn, W, Luz Martins, M, Albuquerque, L, Rosado, L, Velho, R, Amorim, J, Ilina, V.N, Kretchikova, O.I, Klyasova, G.A, Rozanova, S.M, Multykh, I.G, Klimko, N.N, Agapova, E.D, Dmitrieva, N.V, Al-Rasheed, A.M, Shibl, A, Trupl, J, Helena, H, Hoosen, A, Wadula, J, Janse van Rensburg, M.N, Duse, A, Lee, K, Kim, M.-N, del Palacio, A, Sanchez-Sousa, A, Bille, J, Muhlethaler, K, Chang, S.-C, Wang, J.-H, Gur, D, Korten, V, Paul, J, Brown, D, Kibbler, C, Weightman, N, Gould, I.M, Rennison, C, Barnes, R, Vazquez, J, Larone, D, Reyes, H, Santiago, A., and İç Hastalıkları

Subjects
Microbiology (medical), Antifungal Agents, Asia, Microbial Sensitivity Tests, Mycology, Biology, Flucytosine, Microbiology, Middle East, chemistry.chemical_compound, Drug Resistance, Fungal, Amphotericin B, Candida krusei, polycyclic compounds, medicine, Humans, Candida, Voriconazole, Geography, Broth microdilution, Candidiasis, Micafungin, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, Europe, Latin America, chemistry, Hematologic Neoplasms, Africa, North America, Anidulafungin, Caspofungin, medicine.drug
Abstract

Candida krusei is well known as a fungal pathogen for patients with hematologic malignancies and for transplant recipients. Using the ARTEMIS Antifungal Surveillance Program database, we describe geographic and temporal trends in the isolation of C. krusei from clinical specimens and the in vitro susceptibilities of 3,448 isolates to voriconazole as determined by CLSI (formerly NCCLS) disk diffusion testing. In addition, we report the in vitro susceptibilities of bloodstream infection isolates of C. krusei to amphotericin B (304 isolates), flucytosine (254 isolates), anidulafungin (121 isolates), caspofungin (300 isolates), and micafungin (102 isolates) as determined by CLSI broth microdilution methods. Geographic differences in isolation were apparent; the highest frequency of isolation was seen for the Czech Republic (7.6%) and the lowest for Indonesia, South Korea, and Thailand (0 to 0.3%). Overall, 83% of isolates were susceptible to voriconazole, ranging from 74.8% in Latin America to 92.3% in North America. C. krusei was most commonly isolated from hematology-oncology services, where only 76.7% of isolates were susceptible to voriconazole. There was no evidence of increasing resistance of C. krusei to voriconazole from 2001 to 2005. Decreased susceptibilities to amphotericin B (MIC at which 90% of isolates were inhibited [MIC 90 ], 4 μg/ml) and flucytosine (MIC 90 , 16 μg/ml) were noted, whereas 100% of isolates were inhibited by ≤2 μg/ml of anidulafungin (MIC 90 , 0.06 μg/ml), micafungin (MIC 90 , 0.12 μg/ml) or caspofungin (MIC 90 , 0.25 μg/ml). C. krusei is an uncommon but multidrug-resistant fungal pathogen. Among the systemically active antifungal agents, the echinocandins appear to be the most active against this important pathogen.

Published
2008

3. Candida krusei, a Multidrug-Resistant Opportunistic Fungal Pathogen: Geographic and Temporal Trends from the ARTEMIS DISK Antifungal Surveillance Program, 2001 to 2005

Author

Pfaller, M. A., Diekema, D. J., Gibbs, D. L., Newell, V. A., Nagy, E., Dobiasova, S., Rinaldi, M., Barton, R., and Veselov, A.

Abstract

ABSTRACTCandida kruseiis well known as a fungal pathogen for patients with hematologic malignancies and for transplant recipients. Using the ARTEMIS Antifungal Surveillance Program database, we describe geographic and temporal trends in the isolation of C. kruseifrom clinical specimens and the in vitro susceptibilities of 3,448 isolates to voriconazole as determined by CLSI (formerly NCCLS) disk diffusion testing. In addition, we report the in vitro susceptibilities of bloodstream infection isolates of C. kruseito amphotericin B (304 isolates), flucytosine (254 isolates), anidulafungin (121 isolates), caspofungin (300 isolates), and micafungin (102 isolates) as determined by CLSI broth microdilution methods. Geographic differences in isolation were apparent; the highest frequency of isolation was seen for the Czech Republic (7.6%) and the lowest for Indonesia, South Korea, and Thailand (0 to 0.3%). Overall, 83% of isolates were susceptible to voriconazole, ranging from 74.8% in Latin America to 92.3% in North America. C. kruseiwas most commonly isolated from hematology-oncology services, where only 76.7% of isolates were susceptible to voriconazole. There was no evidence of increasing resistance of C. kruseito voriconazole from 2001 to 2005. Decreased susceptibilities to amphotericin B (MIC at which 90% of isolates were inhibited [MIC90], 4 µg/ml) and flucytosine (MIC90, 16 µg/ml) were noted, whereas 100% of isolates were inhibited by =2 µg/ml of anidulafungin (MIC90, 0.06 µg/ml), micafungin (MIC90, 0.12 µg/ml) or caspofungin (MIC90, 0.25 µg/ml). C. kruseiis an uncommon but multidrug-resistant fungal pathogen. Among the systemically active antifungal agents, the echinocandins appear to be the most active against this important pathogen.

Published
2008
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7. In Silico and In Vitro Studies of Mycotoxins and Their Cocktails; Their Toxicity and Its Mitigation by Silibinin Pre-Treatment.

Author

Tran VN, Viktorova J, Augustynkova K, Jelenova N, Dobiasova S, Rehorova K, Fenclova M, Stranska-Zachariasova M, Vitek L, Hajslova J, and Ruml T

Subjects
ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, Animals, Cell Line, Cell Survival drug effects, Coculture Techniques, Comet Assay, Computer Simulation, Dietary Supplements, Drug Interactions, Humans, Mice, Silybum marianum chemistry, Mycotoxins toxicity, Protective Agents pharmacology, Silybin pharmacology
Abstract

Mycotoxins found in randomly selected commercial milk thistle dietary supplement were evaluated for their toxicity in silico and in vitro. Using in silico methods, the basic physicochemical, pharmacological, and toxicological properties of the mycotoxins were predicted using ACD/Percepta. The in vitro cytotoxicity of individual mycotoxins was determined in mouse macrophage (RAW 264.7), human hepatoblastoma (HepG2), and human embryonic kidney (HEK 293T) cells. In addition, we studied the bioavailability potential of mycotoxins and silibinin utilizing an in vitro transwell system with differentiated human colon adenocarcinoma cells (Caco-2) simulating mycotoxin transfer through the intestinal epithelial barrier. The IC 50 values for individual mycotoxins in studied cells were in the biologically relevant ranges as follows: 3.57-13.37 nM (T-2 toxin), 5.07-47.44 nM (HT-2 toxin), 3.66-17.74 nM (diacetoxyscirpenol). Furthermore, no acute toxicity was obtained for deoxynivalenol, beauvericin, zearalenone, enniatinENN-A, enniatin-A1, enniatin-B, enniatin-B1, alternariol, alternariol-9-methyl ether, tentoxin, and mycophenolic acid up to the 50 nM concentration. The acute toxicity of these mycotoxins in binary combinations exhibited antagonistic effects in the combinations of T-2 with DON, ENN-A1, or ENN-B, while the rest showed synergistic or additive effects. Silibinin had a significant protective effect against both the cytotoxicity of three mycotoxins (T-2 toxin, HT-2 toxin, DAS) and genotoxicity of AME, AOH, DON, and ENNs on HEK 293T. The bioavailability results confirmed that AME, DAS, ENN-B, TEN, T-2, and silibinin are transported through the epithelial cell layer and further metabolized. The bioavailability of silibinin is very similar to mycotoxins poor penetration.

Published
2020
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8. Auxarthron ostraviense sp. nov., and A. umbrinum associated with non-dermatophytic onychomycosis.

Author

Hubka V, Dobiasova S, Lyskova P, Mallatova N, Chlebkova J, Skorepova M, Kubatova A, Dobias R, Chudickova M, and Kolarik M

Subjects
Adult, Antifungal Agents pharmacology, Clotrimazole pharmacology, DNA, Fungal chemistry, DNA, Fungal genetics, DNA, Ribosomal Spacer chemistry, DNA, Ribosomal Spacer genetics, Female, Fungal Proteins genetics, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Sequence Data, Naphthalenes pharmacology, Onygenales classification, Onygenales genetics, Psoriasis complications, Sequence Analysis, DNA, Terbinafine, Onychomycosis microbiology, Onygenales isolation & purification
Abstract

Auxarthron is a genus within the Onygenales encompassing keratinophilic species with typical ascomata (gymnothecia) consisting of anastomosing network of thick-walled hyphae and small globose or oblate ascospores. No association of this genus with clinically relevant cases of human or animal infection has been reported. This paper describes the isolation of an undescribed Auxarthron species as an agent of proven onychomycosis affecting almost all fingernails in a man with psoriasis. The causality of the isolated fungus was verified by repeated sampling and direct microscopy revealing irregular septate hyphae. Based on micro- and macromorphological features and unique sequence data (ITS region, benA and RPB2 gene), the isolated fungus is proposed as the new species A. ostraviense. The sibling species of A. ostraviense, A. umbrinum, was isolated from three patients with suspected onychomycosis and a detailed clinical history is provided for one of these patients. All four isolates were tested for susceptibility to selected antifungal agents. Terbinafine and clotrimazole appear to be effective in vitro. The morphological identification of Auxarthron spp. is non-trivial, time-consuming and requires cultivation media other than Sabouraud glucose agar which is routinely used in dermatomycology.

Published
2013
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