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Infectious diseases, bacteria resistance and environmental issues are very serious problems emerged recently in aquaculture and negatively impact to public health. The reason is lack of effectiveness in using antibiotics against the mentioned matters resulting in high residue of antibiotics in exposed animal and human body. Drug delivery nanosystems have been proved as great potential approach to obtain high efficacy in treatment of many types of diseases thanks to excellent ability in delivery and distribution of drug to the targeted sites. In this work Fe3O4based drug delivery nanosystem loading doxycycline was prepared by multi-step procedure. Morphology, size, molecular structure, zeta potential and efficiency of loading antibiotic were determined by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) methods. The results showed that the system have spherical shape and mean size of 103 nm and zeta potential of ?38.9 mV. Therefore, the system was a nanosystem and stable in aqueous dispersion and could load doxycycline antibiotic up to 85.3%. Drug delivery nano system loaded doxycycline showed the efficacy, and prevention of disease on white leg shrimp. This system has the potential to provide effective prevention and treatment of disease in aquaculture.

Nowadays, the raising in agriculture productions is required to meet the demand for growing population. Besides macronutrients (nitrogen, phosphorus and potassium, and to a lesser degree, calcium, sulfur and magnesium) requirements, plants need micronutrients (copper, iron, zinc, boron.) to ensure productivity as well as high agricultural quality. The activity of these elements is greatly affected by very small changes in environmental factors such as pH, mechanical composition and organic matter in the soil. Therefore, there must be another way to effectively bring these elements to the crop. This leads to research and development of a new fertilizer to provide nutrients more effectively. This study reports the synthesis of a new nanofertilizer incorporating micronutrients with hydroxyapatite (nHA) and its impacts on Asparagus officinalisgermination. FESEM images show that the nanofertilizer particles were in rod-shaped with diameters of 20-25 nm and length of approximately 86 nm, while EDX image reveals micronutrient compositions: Ag (696 mg kg?1), Zn (619 mg kg?1), Cu (427 mg kg?1), Co (519 mg kg?1g) and Fe (751 mg kg?1). The 10-day-long Asparagus officinalisseeds test proved a faster germination rate (46.4 ± 1.2 mm/10 days) compared to that of normal treatment (35.3 ± 0.8 mm/10 days). When asparagus was used the micronutrients nano system, they had the ability to grow faster than unused. These results suggest promising application of nanoferitilizer in agriculture.

Recently, high residue of antibiotics in aquaculture products, which is a serious problem in aquaculture industry, has caused not only difficulties for export but also human health problems. In fact, many publications mentioned solutions for alleviating antibiotic content in disease treatment. Application of metal and metal oxides nanoparticles in bacterial treatment has been attracted abundant interests because of their distinctive properties in killing bacteria. For example, TiO2nanoparticles exhibit photocatalytic property in water producing reactive radicals for killing bacteria. Silver nanoparticles can interact with bacterial membrane and molecular structure leading to dysfuction of bacteria. In this study, a drug delivery system combining doxycycline (an antibiotic), TiO2and silver nanoparticles was prepared by multi-step procedure with the aim of improving bacterial treatment effectiveness. Morphology, size, zeta potential, compositions, doxycycline content of the nanoparticles were characterized by field emission scanning electron microscopy (FESEM), x-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and UV-visible spectrometer (UV-Vis) methods. The results showed that doxycycline loaded Ag decorated TiO2nanoparticles have spherical shape with diameter in the range of 50-100 nm, zeta potential of ?34 mV. In vitrotests on White-leg shrimp (Litopenaeus vannamei)infected Vibrio parahaemolyticusdemonstrated the potential of nanoparticles in preventing and treating disease induced by Vibrio parahaemolyticuson white-leg shrimp.

Development of chemoresistance is a significant restriction on the success of cancer treatment. Combination chemotherapy and drug delivery nanosystem are two promising strategies to overcome this limitation. Administration of two or more anticancer drugs at the same time can promote synergistic effect and suppress drug resistance through distinct mechanisms of action. Drug delivery nanosystem, on the other hand, improves delivery, efficacy and safety of drugs, and also can escape from some mechanisms of drug resistance. In this study we prepared drug delivery nanosystems from copolymers of lactic acid (PLA) and d-?-tocopheryl polyethylene glycol 1000 succinate (TPGS). The nanosystems incorporated with folic acid as targeting agent were used to load curcumin (Cur) and paclitaxel (PTX) contemporaneously and denoted as (Cur??+??PTX)-PLA-TPGS-Fol. The results showed that (Cur??+??PTX)-PLA-TPGS-Fol nanoparticles has average size range of 100-200?nm depending on the ratio between PLA and TPGS. Loading efficacy of the two drugs was about 35%-83% with the highest encapsulation efficiency belonged to the system with the highest ratio of PLA. All of the prepared nanosystems with single drug or in combination exhibited strong cytotoxicity to cancer cells, but the combination was more effective in case of A549 cancer cell line. These results showed that our combination of Cur and PTX in our drug delivery nanosystem can be a promising candidate for cancer treatment.

Curcumin is a polyphenol from turmeric Curcuma longaL that has been proved to possess numerous biological and pharmaceutical activities, including anti-cancer properties. However, curcumin has only limited clinical applications due to the aqueous insolubility characteristic that reduces its biological availability. On the other hand, using nanoparticles as drug delivery system has potential as it increases solubility of hydrophobic substances such as curcumin. Furthermore, nanoparticles can protect and control release of drug. Therefore, the objective of this project is to prepare nanoparticles by polymeric encapsulating curcumin by 1-3/1-6 ?-glucan extracted from Vietnamese mushrooms to increase drug delivery efficiency and biological effect. Method of the preparation is nano-precipitation. The produced curcumin-?-glucan-nanoparticles (NanoGluCur) takes spherical shape with 60-70 nm in diameter. As expected, water solubility of curcumin increases about 180 times, from 0.6 ?g ml?1to 0.11 mg ml?1. Loading capacity of NanoGluCur is 18.16%. In vitrocytotoxicity and anti-tumor promoting effects of NanoGluCur were also investigated. Results revealed that NanoGluCur is able to inhibit the growth of two human cancer cell lines Hep-G2 and LU-1 with IC50values of 6.82 and 15.53 mg ml?1, respectively, while free curcumin expresses the activity with IC50values of 7.41 and 18.82 mg ml?1. At the concentration of 40 mg ml?1, NanoGluCur showed anti-tumor promoting effects in reducing tumor size by 59.93% and tumor density by 40.52%, while the percentages caused by pristine curcumin were 41.36% and 29.14%, respectively. These results demonstrated dual effect of 1-3/1-6 ?-glucan encapsulated curcumin nanoparticles: higher water solubility and better in vitroanti-cancer effects compared to free curcumin and 1-3/1-6 ?-glucan, expectedly. This observation can potentially open a new approach in research and manufacture of functional foods from medicinal mushrooms.

Theranostics, which is the combination of both therapeutic and diagnostic capacities in one dose, is a promising tool for both clinical application and research. Although there are many chromophores available for optical imaging, their applications are limited due to the photobleaching property or intrinsic toxicity. Curcumin, a natural compound extracted from the rhizome of curcuma longa, is well known thanks to its bio-pharmaceutical activities and strong fluorescence as biocompatible probe for bio-imaging. In this study, we aimed to fabricate a system with dual functions: diagnostic and therapeutic, based on poly(lactide)-tocopheryl polyethylene glycol succinate (PLA-TPGS) micelles co-loaded curcumin (Cur) and paclitaxel (PTX). Two kinds of curcumin nanoparticle (NP) were fabricated and characterized by Fourier transform infrared spectroscopy, field emission scanning electron microscopy and dynamic light scattering methods. The cellular uptake and fluorescent activities of curcumin in these systems were also tested by bioassay studies, and were compared with paclitaxe-oregon. The results showed that (Cur + PTX)-PLA-TPGS NPs is a potential system for cancer theranostics.