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The aurora arc is a separate auroral structure from the aurora oval, whose location and morphology are related to various solar-terrestrial circumstances. However, because of the low occurring frequency of aurora arc and the lack of the automatic identification technique, it can only be manually distinguished from a huge number of observed images, which is very inefficient. In order to improve the identification efficiency, we propose an identification algorithm based on YOLOX network and Convolutional Block Attention Module attention mechanism. Using the aurora images observed by Special Sensor Ultraviolet Spectrographic Imager carried by the Defense Meteorological Satellite Program F16-F19 satellites from 2013 to 2019, the automatic detection models for global and local areas were trained separately. The identification outputs will be integrated by calculating the intersection. According to the test results, the event identification precision is 86% and the position identification precision is 79%, both of which are greater than the results before integration. Therefore, the proposed method is not only able to identify whether the image contains the aurora arcs, but also accurately locate them, making it a highly effective tool for the advancement of future study. [ABSTRACT FROM AUTHOR]

Dimethylsulfoniopropionate (DMSP) is a vital sulfur-containing compound with worldwide significance, serving as the primary precursor for dimethyl sulfide (DMS), a volatile sulfur compound that plays a role in atmospheric chemistry and influences the Earth's climate on a global scale. The study investigated the ability of four bacterial strains, namely Acidimangrovimonas sediminis MS2-2 (MS2-2), Hartmannibacter diazotrophicus E18T (E18T), Rhizobium lusitanum 22705 (22705), and Nitrospirillum iridis DSM22198 (DSM22198), to produce and degrade DMSP. These strains were assessed for their DMSP synthesis ability with the mmtN linked to non-ribosomal peptide synthase (NRPS) gene. The results showed that MS2-2, and E18T bacteria, which contained the mmtN but not linked to an NRPS gene, increased DMSP production with increasing salinity. The highest production of DMSP was achieved at 25 PSU when either methionine was added or low nitrogen conditions were present, yielding 1656.03 ± 41.04 and 265.59 ± 9.17 nmol/mg protein, respectively, and subsequently under the conditions of methionine addition or low nitrogen, both strains reached their maximum DMSP production at 25 PSU. Furthermore, the strains MS2-2, E18T, and 22705 with the mmtN gene but not linked to an NRPS gene were found to be involved in DMS production. This research contributes to the understanding of the genes involved in DMSP biosynthesis in bacteria that produce DMSP. [ABSTRACT FROM AUTHOR]

Egypt has been reporting several subnational socioeconomic indicators for more than three decades. However, utilizing these valuable datasets for monitoring long temporal trends in local development and inequalities has been hindered by the lack of a key indicator, the Gross Domestic Product (GDP), which was only reported subnationally starting 2013. This paper aims to address this data gap, by employing satellite-generated nighttime lights (NTL) and machine learning, to estimate subnational GDP in Egypt from 1992 to 2012. The paper relies on the harmonized global nighttime lights dataset that extends from 1992 to 2021, to carry out a twofold process. First, it validates NTL as a useful proxy for subnational economic activity in Egypt using econometric methods; then it estimates missing GDP using machine learning algorithms. Results show that the concentration of nearly the entire Egyptian population densely around the Nile River is challenging to nighttime lights accuracy; however, upon accounting for population density and agricultural activity, NTL could serve as a valuable proxy for subnational GDP in Egypt, and consequently a coherent GDP dataset is constructed since 1992. [ABSTRACT FROM AUTHOR]

Human settlements have historically thrived near rivers due to enhanced navigation and trade, and the availability of water supply and resources. The use of night light data, representing economic activities, provides a novel approach to studying the interactions between human activity and rivers over time. Here, we use the Defense Meteorological Satellite Program (DMSP) stable night light data from 2000 to 2013 as a proxy for human presence and activities to quantify the statistical relationships between night light presence and intensity in the Indus Basin, Asia. We test how these data are affected by proximity to trunk channels and by channel type (single/multi-thread) in the study area. We find that night light presence is enhanced by 26% within a 0 to 5 km proximity range of the Indus River and its tributaries, relative to the basin as a whole. We interpret this to represent increased human presence and activity within this zone. However, the mean intensity is lower near the river and higher away from the river, signifying denser settlements, such as towns and cities, which are preferentially located away from the Indus and its tributaries. Moreover, the enhancement of lit pixels signifying human presence and activities is increased by 18% near single-thread sections of the Indus River, compared to segments of the Indus displaying multi-thread morphologies. We suggest that this is due to the enhanced stability of single-threaded channels, relative to mobile multi-threaded channel reaches. This study demonstrates how night lights are an important tool in studying the relationship between human presence and river dynamics in large catchments such as the Indus, and we suggest that these data will have an important role in assessing differential flood spatial and social vulnerability at a regional scale. [ABSTRACT FROM AUTHOR]

Biogenic volatile organic compounds (VOCs) play key roles in coral reef ecosystems, where, together with dimethylated sulfur compounds, they are indicators of ecosystem health and are used as defense strategies and infochemicals. Assessment and prediction of the exchange rates of VOCs between the oceans and atmosphere, with implications for atmospheric reactivity and climate, are hampered by poor knowledge of the regulating processes and their temporal variability, including diel cycles. Here, we measured the variation over 36h of the concentrations of DMSPCs (dimethylsulfoniopropionate (DMSP)-related compounds, namely DMSP, dimethylsulfoxide, acrylate, dimethylsulfide, and methanethiol as dimethyl disulfide) and VOCs (COS, CS2, isoprene, the iodomethanes CH3I and CH2ClI, and the bromomethanes CHBr3 and CH2Br2), in surface waters inside the shallow, northern coral-reef lagoon of Mo'orea (French Polynesia) and 4 km offshore, in the tropical open ocean. Comparisons with concurrent measurements of sea surface temperature, solar radiation, biogeochemical variables (nutrients, organic matter), and the abundances and taxonomic affiliations of microbial plankton were conducted with the aim to explain interconnections between DMSPCs, VOCs, and their environment across diel cycles. In open ocean waters, deeper surface mixing and low nutrient levels resulted in low phytoplankton biomass and bacterial activity. Consequently, the diel patterns of VOCs were more dependent on photochemical reactions, with daytime increases for several compounds including dissolved dimethylsulfoxide, COS, CS2, CH3I, and CH2ClI. A eukaryotic phytoplankton assemblage dominated by dinoflagellates and haptophytes provided higher cell-associated DMSP concentrations, yet the occurrence of DMSP degradation products (dimethylsulfide, dimethyl disulfide) was limited by photochemical loss. Conversely, in the shallow back reef lagoon the proximity of seafloor sediments, corals and abundant seaweeds resulted in higher nutrient levels, more freshly-produced organic matter, higher bacterial activity, and larger algal populations of Mamiellales, diatoms and Cryptomonadales. Consequently, DMSP and dimethylsulfoxide concentrations were lower but those of most VOCs were higher. A combination of photobiological and photochemical processes yielded sunny-daytime increases and nighttime decreases of dimethylsulfide, dimethyl disulfide, COS, isoprene, iodomethanes and bromomethanes. Our results illustrate the important role of solar radiation in DMSPC and VOC cycling, and are relevant for the design of sampling strategies that seek representative and comparable measurements of these compounds. [ABSTRACT FROM AUTHOR]

This article presents a statistical analysis of overlapping double ion-energy dispersion events in the northern cusp ("double dispersion"). Double dispersion in either cusp is a phenomenon associated with multiple reconnections occurring on the dayside magnetosphere as a result of its constant interaction with the variable solar wind. Using observations from a low Earth orbiting (LEO) Defense Meteorological Satellite Program (DMSP) satellite, we analyze 138 dayside events selected by the automatic algorithm extended from our previous work. We conducted a correlation study between the number of detected double dispersion events and 1) the month of the year to analyze the seasonal response of the cusp, and 2) solar wind interplanetary magnetic field (IMF) components and clock/cone angles to investigate its relationship with magnetic reconnection. We found that dispersion events occur more frequently during the northern summer months (i.e., when the dipole is tilted Sunward) and when the By component of IMF is positive. In addition, we provide a machine-readable list of the events and the code used to automatically detect the events. [ABSTRACT FROM AUTHOR]

Proton auroras, when viewed from space, are subjected to Doppler red shifts. Reports on space‐based observations of Doppler shift of H Lyman‐alpha (Ly‐α) spectral line associated with proton precipitation are still scarce. This study, as a first attempt, extracts Doppler shifted H Ly‐α spectral profiles resulting from proton precipitation from data acquired with the Special Sensor Ultraviolet Spectrographic Imager (SSUSI) on board the Defense Meteorological Satellite Program (DMSP)/F16 spacecraft. SSUSI consists of a scanning imaging spectrograph, which covers the far ultraviolet spectrum from ∼1,120 to 1,850 Å, with 160 spectral bins and a spectral resolution of ∼20 Å. We show redshift Ly‐α spectra observed by SSUSI when its field of view traverses the auroral ovals. The inferred proton energy and energy flux are in close agreement with the DMSP SSJ in situ particle measurements. This study demonstrates the potential capability of SSUSI in monitoring the energetics of proton precipitation. Plain Language Summary: Proton precipitation can produce hydrogen lines of emissions known as proton auroras after protons become neutralized and excited through charge exchange with atmospheric hydrogen atoms. Unlike electron auroras, proton auroras are subjected to wavelength shifts called the Doppler effect, because the light emitter is in quick motion. The Doppler shift measurements possibly allows us to estimate precipitating proton energy. When viewed from space, the Doppler effect predicts a shift toward longer wavelengths. As a first attempt, we extract the hydrogen Lyman‐alpha line profile from the Special Sensor Ultraviolet Spectrographic Imager (SSUSI) on board the Defense Meteorological Satellite Program (DMSP) F16 satellite and infer the amount of Doppler wavelength shift to the average energy of the precipitating protons. We compared the result with the SSJ particle detector on board the same DMSP satellite, and they are reasonably in good agreement although they were slightly offset between the two instruments' looking directions. This study demonstrates the potential capability of SSUSI in monitoring the energetics of proton precipitation. Key Points: First report on Doppler shift Lyman‐alpha emissions observed by DMSP/SSUSIThe redshift Lyman‐alpha emissions are associated with auroral proton precipitationThe inferred proton energy flux and mean energy are qualitatively in agreement with particle observations [ABSTRACT FROM AUTHOR]

Satellite‐detected night lights data are widely used to evaluate economic impacts of sanctions. Such data should be free from political manipulation. However, measurement errors in these data, from blurring and bottom‐coding, are rarely considered. To study such errors, we use a difference‐in‐differences analysis of impacts of closing the Kaesong Industrial Zone in North Korea—a sanction South Korea imposed in 2016. Luminosity in the affected region declined by a precisely estimated 50 percent. When using the popular Defense Meteorological Satellite Program (DMSP) night lights data the apparent impacts are imprecisely estimated and far smaller. Measurement errors in DMSP data may distort evaluations of sanctions. [ABSTRACT FROM AUTHOR]

Around 83 percent of the world's population live in light-polluted areas, of which 40 percent live in places that are continuously lit by “ALAN (Artificial Light at Night)”. The light pollution caused by “ALAN” is growing day by day. Thus, the risk of environmental damage is growing in aquatic areas by increasing the “ALAN”. In this way, this research investigates the artificial light at night’s a “ALAN” effect on Gambusia Holbrooke “mosquitofish” in Iran. To obtain the ALAN data, we relied on the information from the operational linear satellite scanning data related to the Metrological Defense Satellite Program (DMSP/OLS). the data provided by the DMSP satellite belonged to 1992 and 2013 as images. The images were clipped by the geographical information systems (GIS), and the images were classified into five classes based on the light pollution intensity. Afterwards, the changes in light pollution by area were calculated and reported. The aim of this research was to examine the effect of ALAN on the Gambusia holbrooki. Two types of treatments were provided for G. holbrooki: control and ALAN treatments. In both treatments, fish were kept for 60 days, and after that period, their shoaling and hiding behaviors were examined and recorded by a web camera. The results indicated that by increasing the ALAN, especially near the coastlines, the risk of harmful effects on G. holbrooki could be increased. The findings suggested that the ALAN might influence G. holbrooki behavior. The shoaling behavior of the ALAN treatments samples revealed that the time spent shoaling individually at night and during the day was decreased. As a result, in the hiding behavior experiment, G. holbrooki moved less at night after ALAN exposure but did not hide, indicating that the fish are unlikely to swim away or hide when exposed to light at night. As a result, they may be more vulnerable to predation. [ABSTRACT FROM AUTHOR]

ABSTRACT Methanethiol (MT) is a sulfur-containing compound produced during dimethylsulfoniopropionate (DMSP) degradation by marine bacteria. The C–S bond of MT can be cleaved by methanethiol oxidases (MTOs) to release a sulfur atom. However, the cleaving process remains unclear, and the species of sulfur product is uncertain. It has long been assumed that MTOs produce hydrogen sulfide (H2S) from MT. Herein, we studied the MTOs in the Rhodobacteraceae family—whose members are important DMSP degraders ubiquitous in marine environments. We identified 57 MTOs from 1,904 Rhodobacteraceae genomes. These MTOs were grouped into two major clusters. Cluster 1 members share three conserved cysteine residues, while cluster 2 members contain one conserved cysteine residue. We examined the products of three representative MTOs both in vitro and in vivo. All of them produced sulfane sulfur other than H2S from MT. Their conserved cysteines are substrate-binding sites in which the MTO–S–S–CH3 complex is formed. This finding clarified the sulfur product of MTOs and enlightened the MTO-catalyzing process. Moreover, this study connected DMSP degradation with sulfane sulfur metabolism, filling a critical gap in the DMSP degradation pathway and representing new knowledge in the marine sulfur cycle field.IMPORTANCEThis study overthrows a long-time assumption that methanethiol oxidases (MTOs) cleave the C–S bond of methanethiol to produce both H2S and H2O2—the former is a strong reductant and the latter is a strong oxidant. From a chemistry viewpoint, this reaction is difficult to happen. Investigations on three representative MTOs indicated that sulfane sulfur (S0) was the direct product, and no H2O2 was produced. Finally, the products of MTOs were corrected to be S0 and H2O. This finding connected dimethylsulfoniopropionate (DMSP) degradation with sulfane sulfur metabolism, filling a critical gap in the DMSP degradation pathway and representing new knowledge in the marine sulfur cycle field.

Biogenic volatile organic compounds (VOCs) play key roles in coral reef ecosystems, where, together with dimethylated sulfur compounds, they are indicators of ecosystem health and are used as defense strategies and infochemicals. Assessment and prediction of the exchange rates of VOCs between the oceans and atmosphere, with implications for atmospheric reactivity and climate, are hampered by poor knowledge of the regulating processes and their temporal variability, including diel cycles. Here, we measured the variation over 36h of the concentrations of DMSPCs (dimethylsulfoniopropionate (DMSP)-related compounds, namely DMSP, dimethylsulfoxide, acrylate, dimethylsulfide, and methanethiol as dimethyl disulfide) and VOCs (COS, CS2, isoprene, the iodomethanes CH3I and CH2ClI, and the bromomethanes CHBr3 and CH2Br2), in surface waters inside the shallow, northern coral-reef lagoon of Mo’orea (French Polynesia) and 4 km offshore, in the tropical open ocean. Comparisons with concurrent measurements of sea surface temperature, solar radiation, biogeochemical variables (nutrients, organic matter), and the abundances and taxonomic affiliations of microbial plankton were conducted with the aim to explain interconnections between DMSPCs, VOCs, and their environment across diel cycles. In open ocean waters, deeper surface mixing and low nutrient levels resulted in low phytoplankton biomass and bacterial activity. Consequently, the diel patterns of VOCs were more dependent on photochemical reactions, with daytime increases for several compounds including dissolved dimethylsulfoxide, COS, CS2, CH3I, and CH2ClI. A eukaryotic phytoplankton assemblage dominated by dinoflagellates and haptophytes provided higher cell-associated DMSP concentrations, yet the occurrence of DMSP degradation products (dimethylsulfide, dimethyl disulfide) was limited by photochemical loss. Conversely, in the shallow back reef lagoon the proximity of seafloor sediments, corals and abundant seaweeds resulted in higher nutrient levels, more freshly-produced organic matter, higher bacterial activity, and larger algal populations of Mamiellales, diatoms and Cryptomonadales. Consequently, DMSP and dimethylsulfoxide concentrations were lower but those of most VOCs were higher. A combination of photobiological and photochemical processes yielded sunny-daytime increases and nighttime decreases of dimethylsulfide, dimethyl disulfide, COS, isoprene, iodomethanes and bromomethanes. Our results illustrate the important role of solar radiation in DMSPC and VOC cycling, and are relevant for the design of sampling strategies that seek representative and comparable measurements of these compounds.

Abstract Proton auroras, when viewed from space, are subjected to Doppler red shifts. Reports on space‐based observations of Doppler shift of H Lyman‐alpha (Ly‐α) spectral line associated with proton precipitation are still scarce. This study, as a first attempt, extracts Doppler shifted H Ly‐α spectral profiles resulting from proton precipitation from data acquired with the Special Sensor Ultraviolet Spectrographic Imager (SSUSI) on board the Defense Meteorological Satellite Program (DMSP)/F16 spacecraft. SSUSI consists of a scanning imaging spectrograph, which covers the far ultraviolet spectrum from ∼1,120 to 1,850 Å, with 160 spectral bins and a spectral resolution of ∼20 Å. We show redshift Ly‐α spectra observed by SSUSI when its field of view traverses the auroral ovals. The inferred proton energy and energy flux are in close agreement with the DMSP SSJ in situ particle measurements. This study demonstrates the potential capability of SSUSI in monitoring the energetics of proton precipitation.

This article presents a statistical analysis of overlapping double ion-energy dispersion events in the northern cusp (“double dispersion”). Double dispersion in either cusp is a phenomenon associated with multiple reconnections occurring on the dayside magnetosphere as a result of its constant interaction with the variable solar wind. Using observations from a low Earth orbiting (LEO) Defense Meteorological Satellite Program (DMSP) satellite, we analyze 138 dayside events selected by the automatic algorithm extended from our previous work. We conducted a correlation study between the number of detected double dispersion events and 1) the month of the year to analyze the seasonal response of the cusp, and 2) solar wind interplanetary magnetic field (IMF) components and clock/cone angles to investigate its relationship with magnetic reconnection. We found that dispersion events occur more frequently during the northern summer months (i.e., when the dipole is tilted Sunward) and when the By component of IMF is positive. In addition, we provide a machine-readable list of the events and the code used to automatically detect the events.

Human settlements have historically thrived near rivers due to enhanced navigation and trade, and the availability of water supply and resources. The use of night light data, representing economic activities, provides a novel approach to studying the interactions between human activity and rivers over time. Here, we use the Defense Meteorological Satellite Program (DMSP) stable night light data from 2000 to 2013 as a proxy for human presence and activities to quantify the statistical relationships between night light presence and intensity in the Indus Basin, Asia. We test how these data are affected by proximity to trunk channels and by channel type (single/multi-thread) in the study area. We find that night light presence is enhanced by 26% within a 0 to 5 km proximity range of the Indus River and its tributaries, relative to the basin as a whole. We interpret this to represent increased human presence and activity within this zone. However, the mean intensity is lower near the river and higher away from the river, signifying denser settlements, such as towns and cities, which are preferentially located away from the Indus and its tributaries. Moreover, the enhancement of lit pixels signifying human presence and activities is increased by 18% near single-thread sections of the Indus River, compared to segments of the Indus displaying multi-thread morphologies. We suggest that this is due to the enhanced stability of single-threaded channels, relative to mobile multi-threaded channel reaches. This study demonstrates how night lights are an important tool in studying the relationship between human presence and river dynamics in large catchments such as the Indus, and we suggest that these data will have an important role in assessing differential flood spatial and social vulnerability at a regional scale.

In situ testing of candidate odorant cues directly in coral-reef communities has the advantage that, in principle, it permits the behavioral responses of all fish species to be evaluated in the species’ natural environments. The success of in situ testing depends critically, however, on a suitable method of odorant release. We provide here a proof-of-concept for a new method of release that (instead of using distilled water as an ejection medium) ejects odorant-containing local reef water remotely and silently over an extended time period using gravity as the motive force. The ejected water, being neutrally buoyant, remains in the reef community, dispersing in local currents. From our observations of fish behavior during ejection of candidate odorants in Curaçao reefs, we confirm that brown chromis (Chromis multilineata) are likely attracted to dimethylsulfoniopropionate (DMSP), and we obtain suggestive evidence that they are attracted to a mix of three betaines: glycine betaine, proline betaine, and trigonelline. Creole wrasse (Clepticus parrae) and yellowhead wrasse (Halichoeres garnoti) are likely repelled by DMSP. Many observed fish species gave no evidence of being affected by either DMSP or betaines.

Dimethylsulfoniopropionate (DMSP) and related organic sulfur compounds play key roles in global sulfur cycling. Bacteria have been found to be important DMSP producers in seawater and surface sediments of the aphotic Mariana Trench (MT). However, detailed bacterial DMSP cycling in the Mariana Trench subseafloor remains largely unknown. Here, the bacterial DMSP-cycling potential in a Mariana Trench sediment core (7.5 m in length) obtained at a 10,816-m water depth was investigated using culture-dependent and -independent methods. The DMSP content fluctuated along the sediment depth and reached the highest concentration at 15 to 18 cm below the seafloor (cmbsf). dsyB was the dominant known DMSP synthetic gene, existing in 0.36 to 1.19% of the bacteria, and was identified in the metagenome-assembled genomes (MAGs) of previously unknown bacterial DMSP synthetic groups such as Acidimicrobiia, Phycisphaerae, and Hydrogenedentia. dddP, dmdA, and dddX were the major DMSP catabolic genes. The DMSP catabolic activities of DddP and DddX retrieved from Anaerolineales MAGs were confirmed by heterologous expression, indicating that such anaerobic bacteria might participate in DMSP catabolism. Moreover, genes involved in methanethiol (MeSH) production from methylmercaptopropionate (MMPA) and dimethyl sulfide (DMS), MeSH oxidation, and DMS production were highly abundant, suggesting active conversions between different organic sulfur compounds. Finally, most culturable DMSP synthetic and catabolic isolates possessed no known DMSP synthetic and catabolic genes, and actinomycetes could be important groups involved in both DMSP synthesis and catabolism in Mariana Trench sediment. This study extends the current understanding of DMSP cycling in Mariana Trench sediment and highlights the need to uncover novel DMSP metabolic genes/pathways in extreme environments. [ABSTRACT FROM AUTHOR]

The microbial cycling of dimethylsulfoniopropionate (DMSP) and the resulting gaseous catabolites dimethylsulfide (DMS) or methylmercaptan (MeSH) play key roles in the global sulfur cycle and potentially climate regulation. As the ocean–atmosphere boundary, the sea surface microlayer (SML) is important for the generation and emission of DMS and MeSH. However, understanding of the microbial DMSP metabolism remains limited in the SML. Here, we studied the spatiotemporal differences for DMS/DMSP, bacterial community structure and the key bacterial DMSP metabolic genes between SML and subsurface seawater (SSW) samples in the eastern China marginal seas (the East China Sea and Yellow Sea). In general, DMSPd and DMSPt concentrations, and the abundance of total, free-living and particle-associated bacteria were higher in SML than that in SSW. DMSP synthesis (~7.81-fold for dsyB, ~2.93-fold for mmtN) and degradation genes (~5.38-fold for dmdA, ~6.27-fold for dddP) detected in SML were more abundant compared with SSW samples. Free-living bacteria were the main DMSP producers and consumers in eastern Chinese marginal sea. Regionally, the bacterial community structure was distinct between the East China Sea and the Yellow Sea. The abundance of DMSP metabolic genes (dsyB, dmdA, and dddP) and genera in the East China Sea were higher than those of the Yellow Sea. Seasonally, DMSP/DMS level and DMSP metabolic genes and bacteria were more abundant in SML of the East China Sea in summer than in spring. Different from those in spring, Ruegeria was the dominant DMSP metabolic bacteria. In conclusion, the DMSP synthesis and degradation showed significant spatiotemporal differences in the SML of the eastern China marginal seas, and were consistently more active in the SML than in the SSW. [ABSTRACT FROM AUTHOR]

Dimethylsulfoniopropionate (DMSP) is a ubiquitous organosulfur compound with key ecological roles in marine environments. This paper offers a brief insight into the mechanisms, environmental diversity, and importance of DMSP-mediated marine microbial interactions, including algae–microzooplankton interactions, bacteria–microzooplankton interactions, and algae–bacteria interactions. We also highlight current challenges that warrant further investigation. [ABSTRACT FROM AUTHOR]

Global Navigation Satellite System's (GNSS) positioning calculation is prone to ionospheric errors. Single frequency GNSS users receive ionospheric corrections through broadcast ionospheric models. Therefore, the accuracy of ionospheric models must be validated based on various geographic and geomagnetic conditions. In this work, an attempt is made to validate NeQuick2 electron density (Ne) using multiple sources of space-based and ground-based data at the Arabian Peninsula and for low solar activity conditions. These sources include space-based data from Swarm, DMSP and COSMIC-2 satellite constellations and ground-based data from GNSS receiver and the ionosonde. The period of this study is 1 year from October 2019 to September 2020. Analysis shows that the agreement between NeQuick2 and experimental Ne close to the peak density height depends on seasons and time of the day with the largest errors found in Autumn and during the daytime. NeQuick2 generally overestimates Ne during the daytime. During the early morning and evening hours, Ne estimates seem to be fairly accurate with slight underestimation in Winter and Spring. Estimation of slab thickness by NeQuick2 is found to be close to the values calculated using collocated ionosonde and GNSS receiver. [ABSTRACT FROM AUTHOR]

Microplastics (MPs) are widespread ocean pollutants and many studies have explored their effects. However, research on MPs combined impact with copper (Cu) on dimethylated sulfur compound production is limited. Dimethyl sulfide (DMS) is an important biogenic sulfur compound related to global temperatures. This study examined the ecotoxicological effects of polyamide 6 MPs and Cu on dimethylsulfoniopropionate (DMSP), DMS, and dimethyl sulfoxide (DMSO) production in Manila clams (Ruditapes philippinarum). Our findings showed that MPs and Cu increased oxidative stress, indicated by higher superoxide anion radical production and malondialdehyde levels while decreasing glutathione contents and increasing superoxide dismutase activities. Additionally, MPs and Cu exposure reduced DMS and dissolved DMSO (DMSO d) concentrations due to decreased grazing. These results contribute to a better understanding of the ecotoxicological effects of MPs/Cu on bivalves and their roles in the organic sulfur cycle, suggesting a need for further research on long-term impacts on them. [Display omitted] • Ecotoxicology effects and dimethylated sulfur yield were assessed under MPs and Cu. • Superoxide dismutase activity increased to defense oxidative stress from MPs/Cu. • R. philippinarum grazing promoted dimethylated sulfur compounds production. • MPs/Cu inhibited dimethyl sulfide yield due to reduced ingestion rates of clams. [ABSTRACT FROM AUTHOR]

On the bow shock in front of Earth’s magnetosphere flows a current due to the curl of the interplanetary magnetic field across the shock. The closure of this current remains uncertain; it is unknown whether the bow shock current closes with the Chapman-Ferraro current system on the magnetopause, along magnetic field lines into the ionosphere, through the magnetosheath, or some combination thereof. We present simultaneous observations from Magnetosphere Multiscale (MMS), AMPERE, and Defense Meteorological Satellite Program (DMSP) during a period of strong By, weakly negative Bz, and very small Bx. This IMF orientation should lead to a bow shock current flowing mostly south to north on the shock. AMPERE shows a current poleward of the Region 1 and Region 2 Birkeland currents flowing into the northern polar cap and out of the south, the correct polarity for bow shock current to be closing along open field lines. A southern Defense Meteorological Satellite Program F18 flyover confirms that this current is poleward of the convection reversal boundary. Additionally, we investigate the bow shock current closure for the above-mentioned solar wind conditions using an MHD simulation of the event. We compare the magnitude of the modeled bow shock current due to the IMF By component to the magnitude of the modeled high-latitude current that corresponds to the real current observed in AMPERE and by Defense Meteorological Satellite Program. In the simulation, the current poleward of the Region 1 currents is about 37% as large as the bow shock Iz in the northern ionosphere and 60% in the south. We conclude that the evidence points to at least a partial closure of the bow shock current through the ionosphere.

Unicellular algae, termed phytoplankton, greatly impact the marine environment by serving as the basis of marine food webs and by playing central roles in the biogeochemical cycling of elements. The interactions between phytoplankton and heterotrophic bacteria affect the fitness of both partners. It is becoming increasingly recognized that metabolic exchange determines the nature of such interactions, but the underlying molecular mechanisms remain underexplored. Here, we investigated the molecular and metabolic basis for the bacterial lifestyle switch, from coexistence to pathogenicity, in Sulfitobacter D7 during its interaction with Emiliania huxleyi, a cosmopolitan bloom-forming phytoplankter. To unravel the bacterial lifestyle switch, we analyzed bacterial transcriptomes in response to exudates derived from algae in exponential growth and stationary phase, which supported the Sulfitobacter D7 coexistence and pathogenicity lifestyles, respectively. In pathogenic mode, Sulfitobacter D7 upregulated flagellar motility and diverse transport systems, presumably to maximize assimilation of E. huxleyi-derived metabolites released by algal cells upon cell death. Algal dimethylsulfoniopropionate (DMSP) was a pivotal signaling molecule that mediated the transition between the lifestyles, supporting our previous findings. However, the coexisting and pathogenic lifestyles were evident only in the presence of additional algal metabolites. Specifically, we discovered that algae-produced benzoate promoted the growth of Sulfitobacter D7 and hindered the DMSP-induced lifestyle switch to pathogenicity, demonstrating that benzoate is important for maintaining the coexistence of algae and bacteria. We propose that bacteria can sense the physiological state of the algal host through changes in the metabolic composition, which will determine the bacterial lifestyle during interaction.

Pc5 ultralow frequency waves are important for transferring energy between the magnetosphere and ionosphere. While many observations have been performed on Pc5 waves properties, it has been difficult to determine the source region, signal propagation path, and the two‐dimensional structure of Pc5 waves beyond coverage by a small number of satellites. Pc5 waves often show a dawn‐dusk asymmetry, but the cause of the asymmetry is under debate. To address these issues, we used conjunction events between the THEMIS satellites and all‐sky imagers and analyzed two Pc5 wave events that were stronger on the dawnside. For both events, the Pc5 waves propagated from dawnside magnetopause toward the nightside magnetosphere. The Pc5 waves were also associated with dawnside magnetopause surface waves, which were probably induced by the Kelvin‐Helmholtz instability. The ionospheric equivalent currents identified multiple vortices on the dawnside associated with quasi‐periodic auroral arcs and much weaker perturbations on the duskside. Global auroral imaging also presented a similar dawn‐dusk asymmetry with multiple arcs on the dawnside, while only one or two major arcs existed on the duskside. Pc5 waves in the magnetosphere had an anti‐phase relation between the total magnetic field and thermal pressure, with a slower propagation velocity compared with magnetohydrodynamic waves. The Poynting flux was anti‐sunward with an oscillating field‐aligned component. These properties suggest that Pc5 waves were slow or drift mirror mode waves coupled with standing Alfven waves. The ground‐based and multi‐satellite observations provide crucial information for determining the Pc5 waves properties, possible source region, and signal propagation path. Plain Language Summary: Pc5 ultralow frequency waves (1.66–6.66 mHz) are large‐scale waves in space, and Pc5 waves are capable of interacting with particles and modulating higher frequency waves, resulting in energy transfer in the magnetosphere‐ionosphere system. However, it is difficult to find the exact signal propagation path and identify the source due to the limited sparse satellite observations in space. We investigated two Pc5 wave events by using the conjunctions of ground magnetometers, all‐sky imagers, and Time History of Events and Macroscale Interaction During Substorms (THEMIS) satellites. We found that the aurora shows periodic intensification and the ionospheric currents had multiple vortices with dawn‐dusk asymmetry. The Pc5 waves in the magnetosphere are identified as standing waves that were coupled with surface waves at the magnetopause traveling anti‐sunward. We suggest that magnetopause surface waves were induced by Kelvin‐Helmholtz instability and drive the Pc5 waves. The dawn‐dusk asymmetry of Kelvin‐Helmholtz instability in the magnetosheath may explain the current asymmetric pattern in the ionosphere. Key Points: Two Pc5 wave events we analyzed were highly dawn‐dusk asymmetric, with stronger magnetic field perturbations on the dawnside than dusksideSlow or drift mirror mode waves coupled with standing Alfven waves in the magnetosphere were driven by the magnetopause surface wavesSurface waves by Kelvin‐Helmholtz instability along the magnetopause is suggested to be the source of the dawnside Pc5 waves [ABSTRACT FROM AUTHOR]

The fluctuations in the electron density of the F-region in the ionosphere as compared to the background electron density are the sign of plasma irregularities which is a well-known phenomenon. They occur during post-sunset hours and degrade ionospheric radio wave communication majorly the navigation signals. To explore the features of equatorial plasma bubbles (EPBs) over the Indian region throughout the solar maximum year 2014 and the solar moderate year 2017, we have examined day and night hours plasma irregularities from Defense Meteorological Satellite Program (DMSP) with spacecraft as F15 with total crossings as 9698. From the total crossings, 1115 crossings were obtained with EPBs degrading the communication and navigation signals. The distribution of EPBs was studied monthly, seasonally, and longitudinally for the solar maximum and moderate years which has given significant results. We have also analyzed the occurrence of EPBs during the geomagnetic storms with Disturbed storm time index (Dst) ≤ -100 nT occurred in the solar maximum and moderate years. We have observed that the regular occurrence of EPBs was there during the initial and main phase but smoothens up in course of the recovery phases of the storm. These results are discussed with those of earlier results and are in good agreement too. The observed inferences will help to improve the telecommunication sector in the Indian region. [ABSTRACT FROM AUTHOR]

Contriving immobilized bioreagent is of great significance to enhance bioremediation of marine oil pollution. However, there remains a notable scarcity of correlational study conducted at deep sea condition. Herein, we first developed a baroduric microsphere encasing biotic and chemical materials to remediate oil-contaminated sediments at deep-sea microcosm. Total oil degradation efficiency of microsphere-treated group reached 71% within a month, representing an approximate 35% increase compared to natural remediation. Absorption and biodegradation by microsphere provided a comparable contribution to oil elimination. Together with scanning electron microscope observation, the physical mechanism was that the reticulate structure of microsphere surface facilitating oil adsorption and bacteria attachment. Via metabarcoding analysis for meta and metabolically-active microbes, we demonstrated the primary working center was located at the microsphere. Proteobacteria, Firmicutes, Bacteroidota and Desulfobacterota were the key activated bacteria. More importantly, we revealed the ecological mechanisms were associated with the following aspects: 1) the addition of microsphere significantly improved the metabolic activity of bacteria (particularly including several oil-degrading taxa); 2) the microspheres enhanced ecological stability and microbial functional diversification during bioremediation; 3) expressing activity of pathways involving oil component degradation, biosurfactant production, biofilm architecture, biogeochemical and energy cycling all were observed to be up-regulated in microsphere-treated samples. Altogether, our results provide important theoretical guidance and data support on application of immobilization technology in removing in-situ oil pollution of deep-sea sediment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

ABSTRACT Dimethylsulfoniopropionate (DMSP) is an abundant organic compound in marine surface water and source of dimethyl sulfide (DMS), the largest natural sulfur source to the upper atmosphere. Marine bacteria either mineralize DMSP through the demethylation pathway or transform it to DMS through the cleavage pathway. Factors that regulate which pathway is utilized are not fully understood. In chemostat experiments, the marine Roseobacter Ruegeria pomeroyi DSS-3 was exposed to oxidative stress either during growth with H2O2 or by mutation of the gene encoding catalase. Oxidative stress reduced expression of the genes in the demethylation pathway and increased expression of those encoding the cleavage pathway. These results are contrary to the sulfur demand hypothesis, which theorizes that DMSP metabolism is driven by sulfur requirements of bacterial cells. Instead, we find strong evidence consistent with oxidative stress control over the switch in DMSP metabolism from demethylation to DMS production in an ecologically relevant marine bacterium. IMPORTANCE Dimethylsulfoniopropionate (DMSP) is the most abundant low-molecular-weight organic compound in marine surface water and source of dimethyl sulfide (DMS), a climatically active gas that connects the marine and terrestrial sulfur cycles. Marine bacteria are the major DMSP consumers, either generating DMS or consuming DMSP as a source of reduced carbon and sulfur. However, the factors regulating the DMSP catabolism in bacteria are not well understood. Marine bacteria are also exposed to oxidative stress. RNA sequencing (RNA-seq) experiments showed that oxidative stress induced in the laboratory reduced expression of the genes encoding the consumption of DMSP via the demethylation pathway and increased the expression of genes encoding DMS production via the cleavage pathway in the marine bacterium Ruegeria pomeroyi. These results support a model where DMS production in the ocean is regulated in part by oxidative stress.

Volatile organic compounds (VOCs) are constituents of marine ecosystems including coral reefs, where they are sources of atmospheric reactivity, indicators of ecosystem state, components of defense strategies, and infochemicals. Most VOCs result from sunlight-related processes; however, their light-driven dynamics are still poorly understood. We studied the spatial variability of a suite of VOCs, including dimethylsulfide (DMS), and the other dimethylsulfoniopropionate-derived compounds (DMSPCs), namely, DMSP, acrylate, and dimethylsulfoxide (DMSO), in waters around colonies of two scleractinian corals (Acropora pulchra and Pocillopora sp.) and the brown seaweed Turbinaria ornata in Mo’orean reefs, French Polynesia. Concentration gradients indicated that the corals were sources of DMSPCs, but less or null sources of VOCs other than DMS, while the seaweed was a source of DMSPCs, carbonyl sulfide (COS), and poly-halomethanes. A focused study was conducted around an A. pulchra colony where VOC and DMSPC concentrations and free-living microorganism abundances were monitored every 6 h over 30 h. DMSPC concentrations near the polyps paralleled sunlight intensity, with large diurnal increases and nocturnal decrease. rDNA metabarcoding and metagenomics allowed the determination of microbial diversity and the relative abundance of target functional genes. Seawater near coral polyps was enriched in DMS as the only VOC, plus DMSP, acrylate, and DMSO, with a large increase during the day, coinciding with high abundances of symbiodiniacean sequences. Only 10 cm below, near the coral skeleton colonized by a turf alga, DMSPC concentrations were much lower and the microbial community was significantly different. Two meters down current from the coral, DMSPCs decreased further and the microbial community was more similar to that near the polyps than that near the turf alga. Several DMSP cycling genes were enriched in near-polyp with respect to down-current waters, namely, the eukaryotic DMS production and DMS oxidation encoding genes, attributed to the coral and the algal symbiont, and the prokaryotic DMS production gene dddD, harbored by coral-associated Gammaproteobacteria. Our results suggest that solar radiation-induced oxidative stress caused the release of DMSPCs by the coral holobiont, either directly or through symbiont expulsion. Strong chemical and biological gradients occurred in the water between the coral branches, which we attribute to layered hydrodynamics.

The history of satellite remote sensing of nighttime lights traces it is roots back the U.S. Air Force Defense Meteorological Satellite Program (DMSP) which began flying polar orbiting satellites with cloud imaging sensors in the 1970’s. The meteorologists found that they could improve weather predictions with visible and thermal band imagery. The visible band channels on early DMSP satellites worked well in the daytime—but were unable to see clouds at nights. Visible band low light imaging was added in the mid-1970’s using photomultiplier tubes to amplify the signal, enabling the detection of moonlit clouds. In 1992, NOAA established a digital archive for DMSP data at the National Geophysical Data Center in Boulder, Colorado. The Earth Observation Group (EOG) was formed in 1994 and dedicated it is efforts to making global nighttime light data products for uses by the science and policy communities. Many of the shortcomings of DMSP data were addressed with the NASA/NOAA Visible Infrared Imaging Radiometer Suite (VIIRS) day/night band (DNB), which opened a new era in nighttime light studies. In total, EOG has produced 65 annual global nighttime light products and over 650 monthly products.

The organic sulfur compounds dimethylsulfoniopropionate (DMSP) and dimethyl sulfoxide (DMSO) play major roles in the marine microbial food web and have substantial climatic importance as sources and sinks of dimethyl sulfide (DMS). Seasonal shifts in the abundance and diversity of the phytoplankton and bacteria that cycle DMSP are likely to impact marine DMS (O) (P) concentrations, but the dynamic nature of these microbial interactions is still poorly resolved. Here, we examined the relationships between microbial community dynamics with DMS (O) (P) concentrations during a 2-year oceanographic time series conducted on the east Australian coast. Heterogenous temporal patterns were apparent in chlorophyll a (chl a) and DMSP concentrations, but the relationship between these parameters varied over time, suggesting the phytoplankton and bacterial community composition were affecting the net DMSP concentrations through differential DMSP production and degradation. Significant increases in DMSP were regularly measured in spring blooms dominated by predicted high DMSP-producing lineages of phytoplankton (Heterocapsa , Prorocentrum , Alexandrium , and Micromonas), while spring blooms that were dominated by predicted low DMSP-producing phytoplankton (Thalassiosira) demonstrated negligible increases in DMSP concentrations. During elevated DMSP concentrations, a significant increase in the relative abundance of the key copiotrophic bacterial lineage Rhodobacterales was accompanied by a three-fold increase in the gene, encoding the first step of DMSP demethylation (dmdA). Significant temporal shifts in DMS concentrations were measured and were significantly correlated with both fractions (0.2–2 μm and >2 μm) of microbial DMSP lyase activity. Seasonal increases of the bacterial DMSP biosynthesis gene (dsyB) and the bacterial DMS oxidation gene (tmm) occurred during the spring-summer and coincided with peaks in DMSP and DMSO concentration, respectively. These findings, along with significant positive relationships between dsyB gene abundance and DMSP, and tmm gene abundance with DMSO, reinforce the significant role planktonic bacteria play in producing DMSP and DMSO in ocean surface waters. Our results highlight the highly dynamic nature and myriad of microbial interactions that govern sulfur cycling in coastal shelf waters and further underpin the importance of microbial ecology in mediating important marine biogeochemical processes. [ABSTRACT FROM AUTHOR]

The location of the polar cap boundary is typically determined by using low‐orbit satellite measurements in which the boundary is identified by its unique signature of a sharp decrease in energy and particle flux poleward of the auroral oval. In principle, this decrease in precipitating particles should appear as a concomitant sharp change in auroral luminosity. Based on a few events, Blanchard et al. (1995, https://doi.org/10.1029/94JA02631) suggested that a dramatic gradient in redline aurora may also be an indicator of the polar cap boundary. In recent years, advances in capabilities and the deployment of ground‐based all‐sky imagers have ushered in a new era of auroral measurements. Auroral imaging has moved well beyond the capabilities of the instrumentation in the previous study in terms of both spatial and temporal resolution. We now have access to decades of optical data from arrays spanning a huge spatial range, enabling a fresh examination of the relationship between redline aurora, particle precipitation, and the polar cap open closed boundary. In this study, we use data from the Defense Meteorological Satellite Program satellites in conjunction with the University of Calgary's Redline Emission Geospace Observatory (630.0 nm) data to assess the viability of automated detection of the 2‐dimensional polar cap boundary. Our results exhibit good agreement between the optical and particle polar cap boundary and suggest that a luminosity in redline emission could serve as a reasonable proxy for the location of the electron poleward boundary, while providing both high temporal and spatial resolution maps of the open‐closed boundary. Plain Language Summary: Named for its shape, the auroral oval is an oval‐shaped region located near the north and south poles where the aurora is formed. Studying the location of the equatorward and poleward limits of the auroral oval is fundamental to evaluate the dynamics and responses of the near‐Earth space environment. In particular, the poleward boundary corresponds to a region in which Earth's magnetic field transitions from closed (connected to Earth on both sides) to open field lines (connected to the solar wind on one side); because of this topological change in magnetic field, the location of this boundary is fundamental to understand mass and energy transport in the whole magnetosphere‐ionosphere system. While satellite data can determine the exact location of this boundary, they only provide point measurements. Auroral images are capable of detecting the luminosity around this boundary; however, many qualitative studies consider the region immediately poleward of this luminosity as the oval's poleward boundary. In this paper, we analyze the location of this boundary in a case study by using satellite data combined with auroral imaging data. Key Points: Polar cap boundary determined with Defense Meteorological Satellite Program particle data allows for examination of the boundary in optical redline dataWe find good agreement between the auroral boundary determined by auroral luminosity and those determined by particle fluxLuminosity method can be applied during active geomagnetic conditions, providing high spatial and temporal resolution polar cap boundary [ABSTRACT FROM AUTHOR]

Bacteria have evolved mechanisms that allow them to adapt to changes in osmolarity and some species have adapted to live optimally in high salinity environments such as in the marine ecosystem. Most bacteria that live in high salinity do so by the biosynthesis and/or uptake of compatible solutes, small organic molecules that maintain the turgor pressure of the cell. Osmotic stress response mechanisms and their regulation among marine heterotrophic bacteria are poorly understood. In this review, we discuss what is known about compatible solute metabolism and transport and new insights gained from studying marine bacteria belonging to the family Vibrionaceae.

The organic sulfur compounds dimethylsulfoniopropionate (DMSP) and dimethyl sulfoxide (DMSO) play major roles in the marine microbial food web and have substantial climatic importance as sources and sinks of dimethyl sulfide (DMS). Seasonal shifts in the abundance and diversity of the phytoplankton and bacteria that cycle DMSP are likely to impact marine DMS (O) (P) concentrations, but the dynamic nature of these microbial interactions is still poorly resolved. Here, we examined the relationships between microbial community dynamics with DMS (O) (P) concentrations during a 2-year oceanographic time series conducted on the east Australian coast. Heterogenous temporal patterns were apparent in chlorophyll a (chl a) and DMSP concentrations, but the relationship between these parameters varied over time, suggesting the phytoplankton and bacterial community composition were affecting the net DMSP concentrations through differential DMSP production and degradation. Significant increases in DMSP were regularly measured in spring blooms dominated by predicted high DMSP-producing lineages of phytoplankton (Heterocapsa, Prorocentrum, Alexandrium, and Micromonas), while spring blooms that were dominated by predicted low DMSP-producing phytoplankton (Thalassiosira) demonstrated negligible increases in DMSP concentrations. During elevated DMSP concentrations, a significant increase in the relative abundance of the key copiotrophic bacterial lineage Rhodobacterales was accompanied by a three-fold increase in the gene, encoding the first step of DMSP demethylation (dmdA). Significant temporal shifts in DMS concentrations were measured and were significantly correlated with both fractions (0.2–2 μm and >2 μm) of microbial DMSP lyase activity. Seasonal increases of the bacterial DMSP biosynthesis gene (dsyB) and the bacterial DMS oxidation gene (tmm) occurred during the spring-summer and coincided with peaks in DMSP and DMSO concentration, respectively. These findings, along with significant positive relationships between dsyB gene abundance and DMSP, and tmm gene abundance with DMSO, reinforce the significant role planktonic bacteria play in producing DMSP and DMSO in ocean surface waters. Our results highlight the highly dynamic nature and myriad of microbial interactions that govern sulfur cycling in coastal shelf waters and further underpin the importance of microbial ecology in mediating important marine biogeochemical processes.

Dimethylsulfoniopropionate (DMSP) is an important molecule in the marine sulfur cycle, produced in large amounts by corals and their dinoflagellate endosymbionts, Symbiodiniaceae. Although corals are known to harbour bacteria that can catabolise DMSP, the recent discovery of bacteria capable of producing DMSP in coastal and deep-sea environments raises the possibility of a bacterial contribution to the DMSP output of corals. Here, 157 bacteria associated with four common coral species were isolated and screened for their ability to produce DMSP by targeting dsyB, a key gene involved in DMSP biosynthesis. Approximately 9% (14 out of 157) of the bacterial isolates harboured dsyB, all being members of the Alphaproteobacteria. The ability of these isolates to produce DMSP was confirmed by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) measurements. A dsyB-harbouring strain, Shimia aestuarii AMM-P-2, was selected for genome sequencing. This strain harbours the complete genetic machinery to (i) assimilate sulfate and synthesise the DMSP precursors, cysteine and methionine; (ii) demethylate DMSP and generate methanethiol; (iii) cleave DMSP, generating dimethyl sulfide (DMS) and acrylate; and (iv) utilise or detoxify acrylate. The impacts of varied environmental factors (temperature, salinity, light and UV radiation) on S. aestuarii AMM-P-2 DMSP biosynthesis were characterised. DMSP levels in S. aestuarii AMM-P-2 increased almost two-fold under both hypersaline conditions (40 PSU) and high UV exposure. DMSP catabolism through the cleavage pathway also increased under these conditions, producing the antioxidants DMS and acrylate, a potential response to the oxidative stress generated. Overall, our results reveal that coral-associated bacteria can synthesize DMSP and may therefore contribute to DMSP production by the coral holobiont.

Shimia strain SK013 is an aerobic, Gram-negative, rod shaped alphaproteobacterium affiliated with the Roseobacter group within the family Rhodobacteraceae. The strain was isolated from surface sediment (0-1 cm) of the Skagerrak at 114 m below sea level. The 4,049,808 bp genome of Shimia str. SK013 comprises 3,981 protein-coding genes and 47 RNA genes. It contains one chromosome and no extrachromosomal elements. The genome analysis revealed the presence of genes for a dimethylsulfoniopropionate lyase, demethylase and the trimethylamine methyltransferase (mttB) as well as genes for nitrate, nitrite and dimethyl sulfoxide reduction. This indicates that Shimia str. SK013 is able to switch from aerobic to anaerobic metabolism and thus is capable of aerobic and anaerobic sulfur cycling at the seafloor. Among the ability to convert other sulfur compounds it has the genetic capacity to produce climatically active dimethyl sulfide. Growth on glutamate as a sole carbon source results in formation of cell-connecting filaments, a putative phenotypic adaptation of the surface-associated strain to the environmental conditions at the seafloor. Genome analysis revealed the presence of a flagellum (fla1) and a type IV pilus biogenesis, which is speculated to be a prerequisite for biofilm formation. This is also related to genes responsible for signalling such as N-acyl homoserine lactones, as well as quip-genes responsible for quorum quenching and antibiotic biosynthesis. Pairwise similarities of 16S rRNA genes (98.56 % sequence similarity to the next relative S. haliotis) and the in silico DNA-DNA hybridization (21.20 % sequence similarity to S. haliotis) indicated Shimia str. SK013 to be considered as a new species. The genome analysis of Shimia str. SK013 offered first insights into specific physiological and phenotypic adaptation mechanisms of Roseobacter-affiliated bacteria to the benthic environment.

The acyl-coenzyme A (CoA) dehydrogenase family enzyme DmdC catalyzes the third step in the dimethylsulfoniopropionate (DMSP) demethylation pathway, the oxidation of 3-methylmercaptopropionyl-CoA (MMPA-CoA) to 3-methylthioacryloyl-CoA (MTA-CoA). To study its substrate specificity, the recombinant DmdC1 from Ruegeria pomeroyi was characterized. In addition to MMPA-CoA, the enzyme was highly active with short-chain acyl-CoAs, with Km values for MMPA-CoA, butyryl-CoA, valeryl-CoA, caproyl-CoA, heptanoyl-CoA, caprylyl-CoA, and isobutyryl-CoA of 36, 19, 7, 11, 14, 10, and 149 μM, respectively, and kcat values of 1.48, 0.40, 0.48, 0.73, 0.46, 0.23, and 0.01 s-1, respectively. Among these compounds, MMPA-CoA was the best substrate. The high affinity of DmdC1 for its substrate supports the model for kinetic regulation of the demethylation pathway. In contrast to DmdB, which catalyzes the formation of MMPA-CoA from MMPA, CoA, and ATP, DmdC1 was not affected by physiological concentrations of potential effectors, such as DMSP, MMPA, ATP, and ADP. Thus, compared to the other enzymes of the DMSP demethylation pathway, DmdC1 has only minimal adaptations for DMSP metabolism compared to other enzymes in the same family with similar substrates, supporting the hypothesis that it evolved relatively recently from a short-chain acyl-CoA dehydrogenase involved in fatty acid oxidation. IMPORTANCE We report the kinetic properties of DmdC1 from the model organism R. pomeroyi and close an important gap in the literature. While the crystal structure of this enzyme was recently solved and its mechanism of action described (X. Shao, H. Y. Cao, F. Zhao, M. Peng, et al., Mol Microbiol 111:1057-1073, 2019, https://doi.org/10 .1111/mmi.14211), its substrate specificity and sensitivity to potential effectors was never examined. We show that DmdC1 has a high affinity for other short-chain acylCoAs in addition to MMPA-CoA, which is the natural substrate in DMSP metabolism and is not affected by the potential effectors tested. This evidence supports the hypothesis that DmdC1 possesses few adaptations to DMSP metabolism and likely evolved relatively recently from a short-chain acyl-CoA dehydrogenase involved in fatty acid oxidation. This work is important because it expands our understanding of the adaptation of marine bacteria to the increased availability of DMSP about 250 million years ago. [ABSTRACT FROM AUTHOR]

The identification and development of talent towards expertise is the primary goal for many professional cricket organisations. The purpose of this study was to utilise retrospective County Age Group (CAG; U10–U19) match performance data to develop age-specific benchmarks for young cricketers to achieve senior professional status. Moreover, results were aligned to the Development Model of Sport Participationto better understand the developmental trajectories of bowlers and batters. The study consisted of 251 male players from an English professional first-class county cricket club who were categorised into two skill-sets dependant on their match performance data (bowlers: n = 118; batters: n = 133). Bowling and batting averages, as well as wickets taken and runs scored, were used for analysis. No significant differences were identified for bowlers who were successful and unsuccessful in achieving professional status prior to U17. In contrast, batters who achieved professional status displayed significantly superior match performance data throughout the pathway. Overall, this data: (a) provides evidence of performance targets for young aspiring cricketers to achieve expertise in English cricket, (b) highlights that bowlers and batters typically follow different development trajectories, and (c) offers an analytical tool for key stakeholders to develop an evidence-based talent identification programme. [ABSTRACT FROM AUTHOR]

Dimethylsulfoniopropionate (DMSP) is one of the most abundant organic sulfur compounds in the oceans, which is mainly degraded by bacteria through two pathways, a cleavage pathway and a demethylation pathway. Its volatile catabolites dimethyl sulfide (DMS) and methanethiol (MT) in these pathways play important roles in the global sulfur cycle and have potential influences on the global climate. Intense DMS/DMSP cycling occurs in the Arctic. However, little is known about the diversity of cultivable DMSP-catabolizing bacteria in the Arctic and how they catabolize DMSP. Here, we screened DMSP-catabolizing bacteria from Arctic samples and found that bacteria of four genera (Psychrobacter, Pseudoalteromonas, Alteromonas, and Vibrio) could grow with DMSP as the sole carbon source, among which Psychrobacter and Pseudoalteromonas are predominant. Four representative strains (Psychrobacter sp. K31L, Pseudoalteromonas sp. K222D, Alteromonas sp. K632G, and Vibrio sp. G41H) from different genera were selected to probe their DMSP catabolic pathways. All these strains produce DMS and MT simultaneously during their growth on DMSP, indicating that all strains likely possess the two DMSP catabolic pathways. On the basis of genomic and biochemical analyses, the DMSP catabolic pathways in these strains were proposed. Bioinformatic analysis indicated that most Psychrobacter and Vibrio bacteria have the potential to catabolize DMSP via the demethylation pathway and that only a small portion of Psychrobacter strains may catabolize DMSP via the cleavage pathway. This study provides novel insights into DMSP catabolism in marine bacteria. [ABSTRACT FROM AUTHOR]

Abstract In this study, we have derived field-aligned currents (FACs) from magnetometers onboard the Defense Meteorological Satellite Project (DMSP) satellites. The magnetic latitude versus local time distribution of FACs from DMSP shows comparable dependences with previous findings on the intensity and orientation of interplanetary magnetic field (IMF) B y and B z components, which confirms the reliability of DMSP FAC data set. With simultaneous measurements of precipitating particles from DMSP, we further investigate the relation between large-scale FACs and precipitating particles. Our result shows that precipitation electron and ion fluxes both increase in magnitude and extend to lower latitude for enhanced southward IMF B z , which is similar to the behavior of FACs. Under weak northward and southward B z conditions, the locations of the R2 current maxima, at both dusk and dawn sides and in both hemispheres, are found to be close to the maxima of the particle energy fluxes; while for the same IMF conditions, R1 currents are displaced further to the respective particle flux peaks. Largest displacement (about 3.5°) is found between the downward R1 current and ion flux peak at the dawn side. Our results suggest that there exists systematic differences in locations of electron/ion precipitation and large-scale upward/downward FACs. As outlined by the statistical mean of these two parameters, the FAC peaks enclose the particle energy flux peaks in an auroral band at both dusk and dawn sides. Our comparisons also found that particle precipitation at dawn and dusk and in both hemispheres maximizes near the mean R2 current peaks. The particle precipitation flux maxima closer to the R1 current peaks are lower in magnitude. This is opposite to the known feature that R1 currents are on average stronger than R2 currents.

We use the first data set from the ground‐based TEC and scintillation receiver located at the Norwegian Research Station Troll in Queen Maud Land, Antarctica to analyze in detail the ionospheric response during geomagnetic disturbances on February 26–27 and March 18–19, 2018. By combining the Troll data with complementary measurements (scintillation receivers, magnetometers, SuperDARN, DMSP satellites), we demonstrate that plasma irregularities above this part of Queen Maud Land can be associated with the expansion of the auroral oval, related structuring on its edges, as well as with strong flow shears in the evening and at nighttime, which are further modulated by the energetic particle precipitation. Phase scintillation indices correlate well with the magnetometer data. Key Points: Plasma irregularities over Troll and SANAE‐4 stations in Queen Maud Land, Antarctica are associated with the edges of expanding auroral ovalPlasma irregularities are related to strong plasma flow shears modulated by auroral particle precipitationsIonospheric structuring is reflected in enhanced phase scintillation index [ABSTRACT FROM AUTHOR]

ABSTRACT The SAR116 clade within the class Alphaproteobacteria represents one of the most abundant groups of heterotrophic bacteria inhabiting the surface of the ocean. The small number of cultured representatives of SAR116 (only two to date) is a major bottleneck that has prevented an in-depth study at the genomic level to understand the relationship between genome diversity and its role in the marine environment. In this study, we use all publicly available genomes to provide a genomic overview of the phylogeny, metabolism, and biogeography within the SAR116 clade. This increased genomic diversity has led to the discovery of two subclades that, despite coexisting in the same environment, display different properties in their genomic makeup. One represents a novel subclade for which no pure cultures have been isolated and is composed mainly of single-amplified genomes (SAGs). Genomes within this subclade showed convergent evolutionary trajectories with more streamlined features, such as low GC content (ca. 30%), short intergenic spacers (

Osmolytes are naturally occurring organic compounds that protect cells against various forms of stress. Highly polar, zwitterionic osmolytes are often used by marine algae and bacteria to counteract salinity or temperature stress. We investigated the effect of several stress conditions including different salinities, temperatures, and exposure to organic metabolites released by the alga Tetraselmis striata on the halophilic heterotrophic bacterium Pelagibaca bermudensis. Using ultra-high-performance liquid chromatography (UHPLC) on a ZIC-HILIC column and high-resolution electrospray ionization mass spectrometry, we simultaneously detected and quantified the eleven highly polar compounds dimethylsulfoxonium propionate (DMSOP), dimethylsulfoniopropionate (DMSP), gonyol, cysteinolic acid, ectoine, glycine betaine (GBT), carnitine, sarcosine, choline, proline, and 4-hydroxyproline. All compounds are newly described in P. bermudensis and potentially involved in physiological functions essential for bacterial survival under variable environmental conditions. We report that adaptation to various forms of stress is accomplished by adjusting the pattern and amount of the zwitterionic metabolites.

We provide high‐resolution maps of quasi‐static Poynting flux (PF) in each hemisphere based on nine‐satellite years of Defense Meteorological Satellite Program (DMSP) data. Conjugate comparisons from ∼850 km reveal more quasi‐static PF arriving in the northern hemisphere (NH) than the southern hemisphere (SH). This tendency is clear in the dawn‐dusk sectors and during intervals when Kp < 3, which accounts for ∼80% of the study interval. Summer‐to‐summer comparisons indicate this asymmetry is partially associated with more NH solar illumination, which supports stronger NH field‐aligned currents (FAC). Differing hemispheric FAC configurations may also play a role. Our findings support and broaden earlier reports of similar NH preference for the deposition of Alfvenic PF. Regionally the NH has stronger dusk‐region PF, while the SH has stronger mid‐morning PF. We find PF deposition in the near‐cusp regions that rivals and often exceeds the PF intensity in the auroral zones. Plain Language Summary: One of the responses to solar wind forcing of geospace is the generation of currents that flow along Earth's magnetic fields. These currents exchange information between the magnetosphere and ionosphere about the state of the system. Steady interactions dissipate energy on the large scale as the currents flow through Earth's resistive upper atmosphere. Non‐steady interactions also dissipate energy, but on multiple scales as the magnetosphere and ionosphere "negotiate" how the changes are to be made in the plasmas of both systems. We map medium‐ and large‐scale hemispheric differences in quasi‐static electromagnetic energy (Poynting flux‐‐PF) delivered to the two polar regions. On a multi‐year basis, the northern hemisphere (NH) receives more electromagnetic energy than the southern hemisphere in almost every sector. Use of nine‐satellite years of full‐component electric and magnetic field measurements from the Defense Meteorological Satellite Program (DMSP) spacecraft are key to showing medium‐sized PF structures near the polar cusps. We show PF deposition in the near‐cusp regions that rivals and often exceeds the PF intensity in the auroral zones. Our findings support and broaden a recent report of similar NH preference for the deposition of PF in the noon‐midnight meridian. Key Points: Nine‐satellite years of DMSP data provide large‐scale views of quasi‐steady Poynting flux (PF)in each hemisphere, independentlyStronger solar illumination and magnetic perturbations in the northern hemisphere (NH) appear to be responsible for the PF excessDuring the respective summer seasons in co‐sampled regions, the NH receives 25% more PF than the southern hemisphere [ABSTRACT FROM AUTHOR]

Haptophytes and Dinoflagellates are two cosmopolitan algae associated with dimethylsulfoniopropionate (DMSP) synthesis, which regulates the marine biogenic flux of DMS to the atmosphere with potential effects on global climate. Attempting to reveal the potential impact of atmospheric deposition on the growth of high‐DMSP producers, four bioassay experiments were conducted in the western North Pacific (WNP) by adding aerosols, nutrients, and trace metals. Our results showed that the percentage of high‐DMSP producers increased substantially from coastal seas (<1%) to the open ocean (∼17%) with the dominance of Dinophyceae and Haptophyceae, respectively. Aerosol additions largely increased the percentage of high‐DMSP producing species in the subtropical gyre of WNP. Specifically, atmospheric dissolved inorganic nitrogen, soluble Cu and Fe promoted Dinophyceae taxa, Chrysochromulina, and Phaeocystis and E. huxleyi, respectively. It is very likely that atmospheric deposition could lift the relative abundance of high‐DMSP producers in the vast oligotrophic oceans and potentially contribute to the climate change. Plain Language Summary: Dimethylsulfoniopropionate (DMSP) is an important precursor of dimethyl sulfide (DMS) and metabolite of algae, which may alter the radiative balance and regulate the global climate. We have conducted four bioassay experiments in different regions of the WNP, simulating atmospheric inputs of nutrients and trace metals analogue to their contents in aerosols. The proportion of high‐DMSP producers increased substantially with the distance offshore, and the dominant species transferred from Dinophyceae to Haptophyceae. It was demonstrated that atmospheric deposition could elevate the proportion of high‐DMSP producers in the oligotrophic ocean rather than in coastal seas. Their larger size compared with Cyanobacteria and special mechanism of metal digestion might be the reason. With the intensive human activities and increasing atmospheric deposition, this stimulation effect may be significantly enhanced and the subsequent climate effects are non‐negligible, especially in those oligotrophic regions. Our results may provide new insights into the ecological effects and climate change. Key Points: Dinophyceae and Haptophyceae were the prime high‐dimethylsulfoniopropionate (DMSP) producers in the coastal and oligotrophic oceans, respectivelyAtmospheric deposition could elevate the proportion of high‐DMSP producers in the oligotrophic oceanDissolved inorganic nitrogen and Cu increased the proportion of Chrysochromulina whilst Fe benefited Phaeocystis and E. huxleyi [ABSTRACT FROM AUTHOR]

The aim of this study was to determine the influence of bacteria and organic matter on the occurrence of dimethyl sulfide (DMS) over the shelf life of Nannochloropsis oceanica concentrates. Two concentrates were compared: Control treatment, composed of biomass collected by centrifugation and diluted to a cell density of 5 × 109 cells·mL−1, and a washed concentrate treatment, where the biomass underwent successive centrifugations to remove dissolved organic matter (DOM) and bacteria before being diluted to a cell density of 5 × 109 cells·mL−1. Both concentrates were stored at two temperatures: 23 °C for 10 days and 4 °C for 85 days. Cellular viability, bacterial density, DOM, and DMS concentration were assessed. The washing process was responsible for removing approximately 84 % of the bacterial load from the concentrates. This significant reduction played a crucial role in decreasing the concentrations of DOM and DMS over the storage period. Furthermore, the process did not impair the cellular viability of microalgae. The findings underscore the importance of carefully managing bacterial and organic components in microalgal concentrates, especially when considering the preservation of freshness and cellular viability, which are key factors in applications such as larviculture. • There is an influence of organic load on the appearance of DMS over the storage time of Nannochloropsis oceanica concentrates; • The washing method was described as a way to reduce the organic load of microalgae concentrates; • Washing process did not impair the cellular viability of microalgae. [ABSTRACT FROM AUTHOR]