35 results on '"Dmitrenko OA"'
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2. A New Approach to Establishing the Set of Phages for Typing Methicillin-ResistantStaphylococcus aureus
- Author
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Akatov Ak, Zueva Vs, and Dmitrenko Oa
- Subjects
Electrophoresis ,0301 basic medicine ,Pharmacology ,Staphylococcus aureus ,viruses ,030106 microbiology ,Biology ,medicine.disease_cause ,Methicillin-resistant Staphylococcus aureus ,Virology ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Oncology ,030220 oncology & carcinogenesis ,medicine ,Humans ,Methicillin Resistance ,Pharmacology (medical) ,Typing ,Staphylococcus Phages ,Bacteriophage Typing ,Prophage ,Phage typing - Abstract
A new approach to using experimental phages for typing methicillin-resistant S. aureus (MRSA) non-sensitive to the phages of International Basic Set (IBS) is described. The collection Includes phage 85, modified on a culture of MRSA, and 5 phages induced from MRSA strains isolated in clinics of Moscow in 1975-76. Firstly, the modified phage selects cultures according to the specific character of its restriction-modification system, then the induced phages differentiate the selected strains into 5 groups (1, 2, 3, 4, 5) based on the specificity of the prophages they contain. Group 1 strains can further be differentiated into 5 subgroups (A, B, C, D, E) by additional phages. Forty-one MRSA strains isolated in 1987-90 in various hospitals of Moscow showing no sensitivity to IBS phages, were lysed by the modified phage, 15 of them belonging to Group 2 and isolated in the traumatological hospital, 26 belonging to Group 1 and were circulating in the burn center. Twenty-three strains of Group 1 appertain to subgroup 1B and were isolated over a 4-year period from the burned surface of patients and from the throat of a medical staff carrier.
- Published
- 1991
3. Prevalence of genes encoding exfoliative toxins among Staphylococcus hyicus isolated in Russia and Germany
- Author
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Dmitrenko Oa, J. Alber, T. Kanbar, Michael Zschöck, Reinhard Weiss, Christoph Lämmler, I. A. Shilov, A. V. Voytenko, and Alexander L. Gintsburg
- Subjects
Exfoliative toxin ,biology ,Base Sequence ,Toxin ,Swine ,Staphylococcus ,Molecular Sequence Data ,General Medicine ,medicine.disease_cause ,biology.organism_classification ,Polymerase Chain Reaction ,Microbiology ,Russia ,Exfoliatins ,Epidermitis, Exudative, of Swine ,Genes, Bacterial ,Germany ,Multiplex polymerase chain reaction ,medicine ,Animals ,Exfoliative Toxins ,Amino Acid Sequence ,Gene ,Sequence Alignment ,Staphylococcus hyicus - Abstract
Summary In the present study, previously characterized Staphylococcus hyicus isolated in Russia (n = 23) and Germany (n = 17) were investigated for the prevalence of the exfoliative toxin encoding genes exhA, exhB, exhC and exhD by multiplex PCR resulting in the detection of exhD positive strains among the S. hyicus isolated from pigs with exudative epidermitis in Russia and the detection of exhC and exhD for one and two strains isolated from exudative epidermitis in Germany respectively. The toxin gene negative strains were generally isolated from apparently healthy pigs, from other animals and from specimens where the relation between the isolation of S. hyicus and the clinical symptoms remained unclear. Partial sequencing of the toxin genes of selected exhC and exhD positive strains and comparing the sequencing results with sequences of exhC and exhD reference strains revealed an almost complete identity. The results of the present study were in agreement with the findings of Andresen and Ahrens (J. Appl. Microbiol., 96, 2004, 1265) and Andresen (J. Vet. Rec., 157, 2005, 376) that the presented multiplex PCR could be used to investigate S. hyicus for toxinogenic potential and that there is an association between the presence of toxin genes in S. hyicus strains from exudative epidermitis. However, comparable with the S. hyicus strains isolated in Germany which were investigated previously by Andresen (J. Vet. Rec., 157, 2005, 376), exhD seems to predominate in S. hyicus strains from Russia.
- Published
- 2006
4. Lysogeny of methicillin-resistant Staphylococcus aureus and the role of prophages in transfer of conjugative and non-conjugative plasmids
- Author
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Zueva Vs, Nesterenko Ln, Dmitrenko Oa, and Akatov Ak
- Subjects
Pharmacology ,Staphylococcus aureus ,Strain (chemistry) ,Mitomycin C ,Conjugative plasmid ,Biology ,Staphylococcal Infections ,bacterial infections and mycoses ,medicine.disease_cause ,Virology ,Methicillin-resistant Staphylococcus aureus ,Microbiology ,Infectious Diseases ,Plasmid ,Oncology ,Lysogenic cycle ,Conjugation, Genetic ,medicine ,Humans ,Pharmacology (medical) ,Methicillin Resistance ,Staphylococcus Phages ,Lysogeny ,Prophage ,Plasmids - Abstract
SummaryThe lysogcnicity of 49 strains of methicillin-resistant S. aureus (MRSA) isolated in Moscow clinics in the 1970s and 80s was studied by the method of mitomycin C induction. It was found that one strain had phage of serogroup B, 33 strains had serogroup F phages and 15 strains had phages of both serogroups. In the course of genetic crossing on nitrocellulose filters it was demonstrated that serogroups B and F prophages contained in recipient cells 1) increase the frequency of transfer of conjugative plasmid pG873 and 2) mobilize transfer of non-conjugative plasmids pE994 and rms7.
- Published
- 1991
5. [The modern characteristics of species identification of coagulase-positive bacteria of Staphylococcus genus.]
- Author
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Balbutskaya AA, Dmitrenko OA, and Skvortsov VN
- Subjects
- Animals, Coagulase, Humans, Polymerase Chain Reaction, Staphylococcus enzymology, Staphylococcal Infections diagnosis, Staphylococcus classification
- Abstract
The development of molecular techniques of research in the end of XX century permitted to broaden nomenclature of species forming genus Staphylococcus that nowadays numbers 51 species and 27 sub-species. The pathogenic species of genus have a capacity to coagulate blood plasma of mammals forming group of coagulase-positive staphylococci including 7 species: S. aureus, S. delphini, S. intermedius, S. pseudintermedius, S. lutrae, S. schleiferi ssp. сoagulans, S hyicus. In clinical practice, S.aureus is considered as the most virulent among staphylococci. The cumulated data testifies increasing etiologic significance of other representatives of group of coagulase-positive staphylococci in human and animal infection pathology. The keen attention is needed to be paid to Staphylococcus intermedius of group (SIG), uniting three close kindred species: S. pseudintermedius, S. intermedius, S. delphini. Among them the most broadly prevailed are methicillin-resistant clones of S. pseudintermedius, capable to bring on in patient various pyoinflammatory diseases. The laboratory methods based on phenotype tests, provide no opportunity to differentiate coagulase-positive staphylococci because of significant similarity of phenotype characteristics in certain representatives of this group. Te comparative analysis was implemented concerning efficiency of various methods of species identification of coagulase-positive staphylococci: biochemical, molecular genetic (multi-primer polymerase chain reaction for identifying differences in gene structure of thermonuclease, analysis of polymorphism of lengths of restricting fragments of catalase gene and their sequencing), matrix-activated laser desorptional/ionizing time-of-flight mass-spectrometry (MALDI-ToF MS) with various modes of probe preparation. The analysis was applied to 117 isolates of representatives of SIG, separated from ill and healthy individuals of small domestic animals, clinical isolates form patients of hospitals. The multi-primer polymerase chain reaction permitted to identify 97% of isolates, analysis of polymorphism of lengths of restricting fragments of catalase gene - 100% of isolates that confirms efficiency of molecular genetic methods of analysis. The MALDI-ToF MS requires replenishment data base of mass-spectrometer and application of the mode of preliminary protein extraction of samples fo increasing efficiency of species identification of coagulase-positive staphylococci., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
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6. [SPECIFIC FEATURES OF ECOLOGY, PATHOGENIC PROPERTIES, AND ROLE OF THE STAPHYLOCOCCUS INTERMEDIUS GROUP MEMBERS IN ANIMAL AND HUMAN INFECTIOUS PATHOLOGY.]
- Author
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Dmitrenko OA, Balbutskaya AA, and Skvortsov VN
- Subjects
- Animals, Humans, Drug Resistance, Bacterial, Staphylococcal Infections drug therapy, Staphylococcal Infections epidemiology, Staphylococcus intermedius metabolism, Staphylococcus intermedius pathogenicity, Virulence Factors metabolism
- Abstract
The changes in the nomenclature of species in the genus Staphylococcus, including the most pathogenic cluster of the coagulase-positive staphylococci, are represented. Presently, besides S. aureus, this cluster consists of 6 species: S. intermedius, S. schleiferi ssp. coagulans, S. lutrae, S. hyicus, S. pseudintermedius, and S. delphini. A particular attention was paid to the Staphylococcus intermedius group (SIG), which includes three closely related coagulase-positive bacterial species: S. intermedius, S. pseudintermedius, and S. delphini. The hosts of SIG species are various mammals and birds, which live in a close contact with humans. The current knowledge about the virulence factors and pathogenicity for animals and humans are analyzed. The diffic6lties of the species identification, the features of ecology and epidemiology, antimicrobial resistance were reviewed. The biological features of S. pseudintermedius, which has the greatest similarity with S. aureus, are considered in the context of the properties of newly emerging pathogens.
- Published
- 2016
7. Molecular Mechanisms of Ceftaroline Susceptibility Reduction in Methicillin-Resistant Staphylococcus aureus.
- Author
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Gostev VV, Kalinogorskaya OS, Dmitrenko OA, Tsvetkova IA, and Sidorenko SV
- Abstract
Ceftaroline is a unique cephalosporin with activity against methicillin resistant Staphylococcus aureus (MRSA). It was approved for clinical use in the USA, Europe and Russian Federation since 2010 for the treatment of the skin and soft tissue infection and community-acquired pneumoniae. In the present study there was used molecular typing of 24 isolates of MRSA with reduced susceptibility to ceftaroline. For 8 isolates belonging to different genetic lines (ST8, ST239 and ST228) and requiring MICs there were determined antibiotic concentrations preventing formation of resistant mutants (mutant prevention concentration) and the ranges of the mutant selection window (MSW). The last majority of the isolates with reduced susceptibility to ceftaroline (MIC of 2 mcg/ml) belonged to the clonal line ST228. The whole genome sequencing of two isolates of ST228 showed that they belonged to the epidemic South Germany genetic line and were characterized by the presence of mutations in PBP2a (N146K) and PBP2 (C197Y) responsible for reduced susceptibility. The highest rates of MPC (32 mcg/ml) and MSW (2-16 mcg/ml) were observed in the clinical isolates belonging to the genetic line ST8. The isolates of ST239 and ST228 had the selection window within 2-4 mcg/ml. No dependence of the MIC and MPC/MSW levels was detected.
- Published
- 2016
8. [Structural Polymorphism of Genome Islands Encoding Resistance to Beta-Lactams in Coagulase-Negative Staphylococci Isolated at Hospitals of the Russian Federation].
- Author
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Dmitrenko OA, Ankirskaya AS, Lyubasovskaya LA, Kovalishena OV, Popov DA, Gostev VV, and Sidorenko SV
- Subjects
- Anti-Bacterial Agents pharmacology, Bacterial Proteins metabolism, Bacterial Typing Techniques, Chromosomes, Bacterial chemistry, Gene Expression, Hospitals, Humans, Interspersed Repetitive Sequences, Microbial Sensitivity Tests, Molecular Sequence Data, Recombinases metabolism, Russia, Staphylococcal Infections drug therapy, Staphylococcal Infections microbiology, Staphylococcus drug effects, Staphylococcus isolation & purification, Staphylococcus pathogenicity, beta-Lactams pharmacology, Bacterial Proteins genetics, Genomic Islands, Polymorphism, Genetic, Recombinases genetics, Staphylococcus genetics, beta-Lactam Resistance genetics
- Abstract
Coagulase-negative staphylococci (CoNS) are considered as a reservoir of mobile genetic elements and first of all of the staphylococcal cassette chromosome mec (SCCmec), defining staphylococci resistance to beta-lactams. Types II, IV, IVa, V, VII and VIII SCCmec were detected among 95 staphylococcal strains isolated in different regions of the Russian Federation. Subtypes C1a, C1b, C1c and C1 SCCmec were also identified (class B mec complex and two complexes of ccr1 and ccr2 genes recombinases). Some other cassette types carrying A, C1 and C2 classes of the mec complexes in combination with various recombinase genes were detected. The S.epidermidis isolates mainly formed cassettes carrying mec complex B, while the S. haemolyticus isolates had cassettes carrying classes C1 and C2 mec complex. Out of 9 isolates of S. hominis 5 isolates carried a new type cassette: class A mec complex in combination with the complex of the recombinase ccr1 genes. SCCmec was not identified in S. capitis and S. pasteuri. Their representatives carried either mec complex (1 isolate of S. pasteuri) or the recombinase complexes (2 isolates of S. capitis). The detected SCCmec variants in CoNS could be a source of emergence of new genetic lines of MRSA.
- Published
- 2015
9. xMAP-based analysis of three most prevalent staphylococcal toxins in Staphylococcus aureus cultures.
- Author
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Simonova MA, Petrova EE, Dmitrenko OA, Komaleva RL, Shoshina NS, Samokhvalova LV, Valyakina TI, and Grishin EV
- Subjects
- Culture Media analysis, Exotoxins metabolism, Staphylococcus aureus chemistry, Staphylococcus aureus growth & development, Exotoxins analysis, Immunoassay methods, Staphylococcus aureus metabolism
- Abstract
Detection of staphylococcal toxins presents a great interest for medical diagnostics. Screening of clinical samples for the presence of several types of staphylococcal toxins using traditional methods-biological tests on animals or cell cultures as well as ELISA-is laborious. Multiplex detection methods would simplify testing. We have designed an xMAP-based assay to detect three staphylococcal toxins-enterotoxins A and B (SEA and SEB) and toxic shock syndrome toxin (TSST)-in cultural supernatants obtained from different strains of Staphylococcus aureus. The limits of detection of SEA, SEB, and TSST multiplex detection in S. aureus growth medium were 10, 1,000, and 5 pg/mL, respectively. Fifty-nine samples of S. aureus cultural supernatants were tested with the xMAP assay. The developed assay has proved highly effective detection of the natural toxins in the samples obtained due to bacterial cells cultivation. In prospect, the developed test system can be used in clinical diagnostics and in monitoring of foodstuffs and environmental objects.
- Published
- 2014
- Full Text
- View/download PDF
10. [Detection of Staphylococcus aureus toxins using immuno-PCR].
- Author
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Maerle AV, Riazantsev DIu, Dmitrenko OA, Petrova EIa, Komaleva RL, Sergeev IV, Trofimov DIu, and Zavriev SK
- Subjects
- Bacterial Toxins chemistry, Bacterial Toxins genetics, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Bacterial isolation & purification, Enterotoxins chemistry, Enterotoxins genetics, Enzyme-Linked Immunosorbent Assay, Humans, Shock, Septic diagnosis, Shock, Septic microbiology, Staphylococcal Infections microbiology, Staphylococcus aureus chemistry, Staphylococcus aureus genetics, Staphylococcus aureus pathogenicity, Superantigens chemistry, Superantigens genetics, Bacterial Toxins isolation & purification, Enterotoxins isolation & purification, Polymerase Chain Reaction methods, Staphylococcal Infections diagnosis, Superantigens isolation & purification
- Abstract
A highly sensitive test system, based on the method of immuno-PCR, was developed for the detection of two staphylococcal toxins: enterotoxin A (SEA) and toxic shock syndrome toxin (TSST). A key element of the developed systems was obtaining supramolecular complexes of bisbiotinylated oligodeoxynucleotides and streptavidin, which were used as DNA tags. Specificity studies showed no cross-reactivity when determining SEA and TSST. The sensitivity of detection of these toxins in the culture supernatants S. aureus was not less than 10 pg/mL.
- Published
- 2014
- Full Text
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11. [The architectonics of microbe ecology in the purulent surgery department of municipal clinical hospital].
- Author
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Mironov AIu and Dmitrenko OA
- Subjects
- Bacterial Infections drug therapy, Bacterial Infections microbiology, Drug Resistance, Bacterial genetics, Ecology, Enterobacteriaceae genetics, Enterobacteriaceae isolation & purification, Enterobacteriaceae pathogenicity, Humans, Microbial Sensitivity Tests, Moscow, Anti-Bacterial Agents therapeutic use, Gram-Negative Bacteria classification, Gram-Negative Bacteria drug effects, Gram-Negative Bacteria genetics, Gram-Negative Bacteria isolation & purification, Gram-Positive Bacteria classification, Gram-Positive Bacteria drug effects, Gram-Positive Bacteria genetics, Gram-Positive Bacteria isolation & purification, Hospitals, Municipal, Staphylococcus aureus genetics, Staphylococcus aureus isolation & purification, Staphylococcus aureus pathogenicity
- Abstract
S. aureus, S. pyogenes, P. aerugenosa, E. coli, P mirabilis, A. baumannii, S. epidermidis, K. pneumoniae, E. faecalis, E. cloacae are the priority pathogens of various forms of pyoinflammatory diseases. They form the architectonics of microbe ecology of the purulent surgery department of multi-type hospital of regional level with S.aureus subsp.aureus playing dominating role. In case of unchanged specter of priority pathogens of pyoinflammatory and pyoseptic diseases the number of gram-positive coccuses decreases at the expense of decrease of number of streptococcuses and staphylococcuses. At the same time, the number of enterobacteria and gram-negative non-fermentative bacteria increases. The resistance ofgram-positive coccuses increases regarding erythromycin, clyndamicin and cyprofloxacin. The resistance of gram-negative bacilli increases regarding ciprofloxacin, cephalosporins of III-IV generations, amikacin. The resistance is the highest among clinical isolates MRSA, K. pneumoniae, A. baumannii. The vancomycin is active regarding all gram-positive pathogens. The carbapenems are active regarding all enterobacteriae. The carbapenems, cefoperazone/tazobactam, cefepime are most active regarding non-fermentative glucose oxidizing bacteria. The netimicin is active regarding A.baumannii. The polymyxine is active regarding P. aerugenosa. The circulation of S. aureus hospital strain of particular genotype is established confirming the propagation of epidemic S. aureus strains in Moscow multi-type medical institutions. The strains are genetically affined to epidemic strains in European and other countries according the international data base (http://SpaServer.ridom.de). The genetic typing of S. aureus ssp. aureus out-hospital hemocultures detected their considerable genetic variety. The epidemic relationship between isolates from different patients is not established. The algorithms of rationale antibacterial chemotherapy of pyoinflammatory and pyoseptic diseases are developed to be implemented in the purulent surgery department of municipal clinical hospital of Moscow.
- Published
- 2012
12. [Development of Staphylococcus Haemolyticus multilocus sequencing scheme and its use for molecular-epidemiologic analysis of strains isolated in hospitals in Russian federation in 2009-2010].
- Author
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Voronina OL, Kunda MS, Dmitrenko OA, Lunin VG, and Gintsburg AL
- Subjects
- Base Sequence, Coagulase analysis, Cross Infection epidemiology, Genes, Bacterial, Genotype, Hospitals, Humans, Molecular Sequence Data, Phylogeny, Russia epidemiology, Staphylococcal Infections epidemiology, Staphylococcus haemolyticus classification, Staphylococcus haemolyticus genetics, Bacterial Typing Techniques, Cross Infection microbiology, Sequence Analysis, DNA methods, Staphylococcal Infections microbiology, Staphylococcus haemolyticus isolation & purification
- Abstract
Aim: Development of Staphylococcus haemolyticus strain typing method based on multilocus sequencing for resolving problems of molecular epidemiology., Materials and Methods: 102 strains of coagulase negative staphylococci (CNS) isolated in hospitals of various specialization in N. Novgorod and Moscow were studied. Species identification of strain was performed by using tuf gene fragment sequencing, S. haemolyticus strain differentiation--by MLST results. eBURST approach was used for cluster analysis of MLST data; structural changes in tagatose-6-phosphate kinase were studied by using InterProScan platform and SWISS-MODEL site programs; MLST scheme gene allele variability analysis was performed by using MEGA4.0 program package., Results: In the 102 strains sampled CNS was detected in 28 strains of the S. haemolyticus species. The MLST scheme developed for the first time for S. haemolyticus including mvaK, rphE, tphK, gtr, arcC, triA, aroE genes allowed the differentiation of the sampled strains by 11 genotypes. Strains with ST 3, 8, 6, 1, 4, 5 and 11 differed by highest epidemiologic significance. Cluster and phylogenetic analysis of the data obtained showed a high adaptive ability of the nosocomial S. haemolyticus strains. Multiresistance to antibacterial preparations was detected in the analyzed strains., Conclusion: The MLST method developed was effective in the differentiation of S. haemolyticus strains that circulate in hospitals and threaten both neonates and hospitalized adult patients.
- Published
- 2011
13. Prevalence of genes encoding exfoliative toxins among Staphylococcus hyicus isolated in Russia and Germany.
- Author
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Kanbar T, Voytenko AV, Alber J, Lämmler C, Weiss R, Zschöck M, Shilov IA, Dmitrenko OA, and Gintsburg AL
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Epidermitis, Exudative, of Swine metabolism, Exfoliatins metabolism, Germany, Molecular Sequence Data, Polymerase Chain Reaction methods, Polymerase Chain Reaction veterinary, Russia, Sequence Alignment, Staphylococcus metabolism, Swine, Epidermitis, Exudative, of Swine microbiology, Exfoliatins genetics, Genes, Bacterial, Staphylococcus genetics
- Abstract
In the present study, previously characterized Staphylococcus hyicus isolated in Russia (n=23) and Germany (n=17) were investigated for the prevalence of the exfoliative toxin encoding genes exhA, exhB, exhC and exhD by multiplex PCR resulting in the detection of exhD positive strains among the S. hyicus isolated from pigs with exudative epidermitis in Russia and the detection of exhC and exhD for one and two strains isolated from exudative epidermitis in Germany respectively. The toxin gene negative strains were generally isolated from apparently healthy pigs, from other animals and from specimens where the relation between the isolation of S. hyicus and the clinical symptoms remained unclear. Partial sequencing of the toxin genes of selected exhC and exhD positive strains and comparing the sequencing results with sequences of exhC and exhD reference strains revealed an almost complete identity. The results of the present study were in agreement with the findings of Andresen and Ahrens (J. Appl. Microbiol., 96, 2004, 1265) and Andresen (J. Vet. Rec., 157, 2005, 376) that the presented multiplex PCR could be used to investigate S. hyicus for toxinogenic potential and that there is an association between the presence of toxin genes in S. hyicus strains from exudative epidermitis. However, comparable with the S. hyicus strains isolated in Germany which were investigated previously by Andresen (J. Vet. Rec., 157, 2005, 376), exhD seems to predominate in S. hyicus strains from Russia.
- Published
- 2006
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14. Identification of Staphylococcus hyicus by polymerase chain reaction mediated amplification of species specific sequences of superoxide dismutase A encoding gene sodA.
- Author
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Voytenko AV, Kanbar T, Alber J, Lämmler C, Weiss R, Prenger-Berninghoff E, Zschöck M, Akineden O, Hassan AA, and Dmitrenko OA
- Subjects
- Phylogeny, Polymerase Chain Reaction, Bacterial Proteins genetics, Staphylococcus classification, Staphylococcus genetics, Superoxide Dismutase genetics
- Abstract
A species specific PCR test, based on manganese-dependent superoxide dismutase A encoding gene sodA, was developed for the identification of Staphylococcus hyicus, an important bacterial pathogen in pigs. The designed primers allowed a rapid and reliable identification of phenotypically characterized S. hyicus, isolated in Russia, Germany and Denmark. No cross reactivities could be observed investigating staphylococcal reference strains representing 18 different species and subspecies. The use of the described primers might improve a future diagnosis of this bacterial pathogen.
- Published
- 2006
- Full Text
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15. [Detection of the genes of pyrogenic toxins of superantigens in clinical isolates of methicillin resistant Staphylococcus aureus].
- Author
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Dmitrenko OA, Prokhorov VIa, Fluer FS, Suborova TN, Volkov II, Karabak VI, and Gintsburg AL
- Subjects
- Anti-Bacterial Agents pharmacology, Bacteremia epidemiology, Bacterial Toxins genetics, Drug Resistance, Bacterial, Enterotoxins genetics, Genetic Markers genetics, Hospitals, Humans, Methicillin pharmacology, Molecular Epidemiology, Polymerase Chain Reaction, Russia epidemiology, Shock, Septic epidemiology, Staphylococcus aureus drug effects, Staphylococcus aureus immunology, Superantigens genetics, Cross Infection epidemiology, Disease Outbreaks, Staphylococcal Infections epidemiology, Staphylococcus aureus genetics
- Abstract
The content of methicillin resistant S. aureus (MRSA) genes, coding the synthesis of staphylococcal enterotoxins A, B, C (sea, seb, sec) and the toxin of the toxic shock syndrome (tst-H) which was classified with pyrogenic toxins of superantigens (PTSAgs), was studied with the use of PCR amplification. The study revealed the specific features of the content of genes sea and sec, detected in epidemic strains, identified earlier and found to circulate in Russian hospitals. Among the isolates, genetically related to international epidemic strain EMRSA-1, isolates containing no gene sea were detected, while among the isolates genetically related to strain EMRSA-2, isolates containing not only gene sea, but also gene sec were detected, which was indicative of the tendence of this epidemic strain in the direction of further acquisition of pathogenicity genes. As revealed in further studies, among the cultures obtained in bacteriemia, 88% contained gene sea. Two out of three isolates obtained from patients with the symptoms of toxic shock also contained this gene. The differences in the content of genes PTSAgs (sea, seb, sec and tst-H) could serve as a genetic criterium for the differention of isolates circulating in a hospital, as well as for a more complete characterization of the epidemic strains MRSA. The determination of the given genetic markers in genetic strains in circulating strains will make it possible to prognosticate the structure, severity and outcomes of hospital infections. The conditions of PCR amplification for the determination of genes sea, seb, sec and tst-H, as well as multiplex PCR for the determination of genes sea and seb, were developed.
- Published
- 2006
16. [Molecular genetic typing of methicillin-resistant Staphylococcus aureus strains, isolated in hospitals of different regions of the Russia and Belarus].
- Author
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Dmitrenko OA, Shaginian IA, Prokhorov VIa, Matveev SM, and Gintsburg AL
- Subjects
- Bacterial Proteins genetics, Bacterial Typing Techniques methods, Coagulase genetics, Cross Infection epidemiology, Cross Infection microbiology, Hospitals, Community, Humans, Molecular Epidemiology, Polymorphism, Restriction Fragment Length, Republic of Belarus epidemiology, Russia epidemiology, Staphylococcal Infections microbiology, Staphylococcus aureus drug effects, Staphylococcus aureus enzymology, Staphylococcus aureus genetics, Methicillin Resistance genetics, Staphylococcal Infections epidemiology, Staphylococcus aureus classification
- Abstract
The study of the length of the amplification products of the coagulase gene with the subsequent restriction analysis (the method of PCR--restrictive fragment length polymorphism, or RFLP) was used for typing 90 S. aureus strains. Among the strains under study, 78 were methicillin-resistant S. aureus (MRSA) strains, including 74 obtained in 1986 - 2002 in hospitals of different cities of the Russia and Belarus, as well as epidemic strains EMRSA-1, -2, -3, -12, obtained from the National Laboratory of Health, London (UK). The use of this method made it possible to type all the strains under study, which were differentiated into 9 groups by means of endonuclease Sfo1 and 7 groups by means of Alu1. Majority of clinical MRSA strains, belonged, according to the type of restriction, to groups 4 and 5. The study of the coagulase gene by the method of PCR - RFLP made it possible: to analyze the epidemic situation in hospitals for a period of several years; to compare the properties of strains isolated in different hospitals; to establish the genetic relationship of strains, isolated in 1998 - 2002, with strains, isolated in 1986 - 1990. The results of the study suggest that at least two epidemic MRSA strains, genetically similar to international strains, circulate in hospitals of Russia.
- Published
- 2005
17. [A study of the polymorphism of mec dna in methycillin-resistant strains of Staphylococcus aureus isolated at permanent stations in different regions of Russia].
- Author
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Dmitrenko OA, Shaginian IA, and Gintsburg AL
- Subjects
- Coagulase genetics, DNA Transposable Elements genetics, DNA, Bacterial genetics, Polymerase Chain Reaction, Russia, Staphylococcus aureus drug effects, Staphylococcus aureus isolation & purification, Cross Infection microbiology, Genes, Bacterial, Methicillin Resistance genetics, Polymorphism, Genetic, Staphylococcal Infections microbiology, Staphylococcus aureus genetics
- Abstract
Polymorphism of the chromosome staphylococcus cassette mec (SCCmec), a mobile and heterological genetic element providing resistance to beta-lactam antibiotics was studied in methycillin-resistant strains of Staphylococcus aureus (MRSA) isolated at permanent stations situated in different regions of Russia. Type SCCmec was identified using the PCR method by determining allotypes of 3 different structural genetic complexes incorporated in the cassettes mec. It was found that the isolates studied in this work contained 3 different types of SCCmec: I, III, and IVb. Both isolates containing 2 different copies of SCCmec and isolates containing defective copies of SCCmec were identified. It was demonstrated that determination of the SCC-mec type provided an opportunity to differentiate the isolates studied in this work from one another. The isolates attributed to the same genotype variant (identified by polymorphism of coagulase gene) but isolated at different hospitals located in different regions of Russia were found to contain the same type of the chromosome staphylococcus cassette mec, whereas the isolates of different coagulase groups (i.e., different genotype variants) contained different types of SCCmec. It was found that at least 2 epidemic strains circulated in the permanent hospitals of Russia. The strains differ from one another by the polymorphism of the coagulase gene and the mec DNA polymorphism. According to results of studies of several molecular markers (including mec DNA), these strains proved to be identical to the international strains EMRSA-1 and EMRSA-2. Possible mechanisms of MRSA formation and circulation in Russia and CIS countries are discussed.
- Published
- 2005
18. [A system of step-by-step differentiation of methicillin-resistant Staphylococcus aureus].
- Author
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Zueva VS, Dmitrenko OA, Shaginian IA, and Akatov AK
- Subjects
- Bacteriophage Typing, Genotype, Phenotype, Reproducibility of Results, Genetic Testing methods, Methicillin Resistance genetics, Staphylococcus aureus genetics
- Abstract
One hundred and eighty four strains of methicillin resistant Staphylococcus aureus (MRSA) isolated in pyosurgical and burn departments of Moscow, Minsk, Omsk, Tbilisi, Vologda, Smolensk and Dushanbe were differentiated with using phages of the International Set (BIS), two collections of experimental phages and two-probe fingerprinting. More than 50 per cent of the isolates could not be typed by the BIS phages. The Experimental Collection of the N.F. Gamaleya Research Institute of Epidemiology and Microbiology (Moscow) was more useful in the differentiation in comparison to the Experimental Collection of the London Health Centre. A new approach applied by us to the establishment of our collection i.e. screening of cultures with a definite specificity of the system of the restriction-modification (by a modified phage 85) followed by their differentiation with respect to the specificity of the prophages (by the induced phages with the respective modification specificity) provided reliable and reproducible results. Our collection made it possible to classify the phage type 85 strains which as was confirmed by the fingerprinting data belonged to 3 different genotype variants. The fingerprinting had no advantages over the typing by the phages of the more extended phage set and was recommended for differentiation of the strains (14 per cent) not sensitive to the phages used. A step-by-step scheme for typing MRSA easy for use in clinical and epidemiological laboratories is described.
- Published
- 1997
19. [The epidemiological surveillance of hospital infections linked to methicillin-resistant Staphylococcus aureus].
- Author
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Musina LT, Semina NA, Gladkova KK, Dmitrenko OA, Dalmatov VV, Obukhova TM, Stasenko VA, Nikolaeva TN, Adarchenko AA, and Gladkova EG
- Subjects
- Anti-Bacterial Agents pharmacology, Bacteriophage Typing, Cross Infection microbiology, Humans, Microbial Sensitivity Tests, Russia epidemiology, Seasons, Staphylococcal Infections microbiology, Staphylococcus aureus classification, Staphylococcus aureus drug effects, Cross Infection epidemiology, Methicillin Resistance, Population Surveillance, Staphylococcal Infections epidemiology, Staphylococcus aureus isolation & purification
- Abstract
As revealed in the realization of the epidemiological surveillance of hospital infections caused by methicillin-resistant S. aureus (MRSA) in different types of hospital, MRSA strains causing purulent inflammatory diseases belong to different clones. The complex marking of MRSA made it possible to determine the presence of the same clone in different hospitals and to detect the outbreaks of hospital infections caused by different clones of MRSA in one hospital. It was found necessary to supplement the commercial international phage-typing set with pages permitting the detection of the specific system of restriction-modification in MRSA.
- Published
- 1995
20. [Additional differentiation of methicillin resistant strains of Staphylococcus aureus typed by the international phage bank].
- Author
-
Zyeva VS, Dmitrenko OA, Akatov AK, and Gladkova KK
- Subjects
- Bacteriophage Typing, Microbial Sensitivity Tests, Methicillin Resistance physiology, Staphylococcus aureus drug effects
- Abstract
In the methicillin resistant strains of Staphylococcus aureus (MRSA) typed by the International Set phages the host specificity of the restriction-modification of the phage 85 DNA was determined, the finger printing of the cell DNA was carried out with using two probes and the lytic spectrum of the phages induced in them was studied. Four clones with different specificity of the restriction-modification system (rm89, rm108, rm121 and rm947) differing from that of strain PS 85 which is the host of phage 85 were detected. The strains belonging to the modification types m89, m108 and m121 contained prophages (within the respective groups) with similar lytic spectra when tested with the use of the PS strains of the International Collection and had cross antiphage immunity. Six phage variants were detected among the phages induced in the strains with the modification type m947 which could be indicative of the clone heterogeneity.
- Published
- 1994
21. [Experimental substantiation of population heterogeneity in methicillin resistant Staphylococcus aureus].
- Author
-
Musina LT, Gladkova KK, Dmitrenko OA, Gladkova EG, Semina IE, Petrash P, Dalmatov VV, Nikolaeva TN, Reshed'ko GK, and Adarchenko AA
- Subjects
- Bacterial Typing Techniques, Bacteriophages physiology, Species Specificity, Staphylococcus aureus classification, Staphylococcus aureus virology, Methicillin Resistance genetics, Staphylococcus aureus drug effects
- Abstract
A comprehensive intraspecies typing of the cultures of MRSA collected during inspection of drug resistance in causative agents of intrahospital infections was performed. The following parameters were investigated: antibiotic resistance, toxin production, sensitivity to the phages of the International Set and the phages of an experimental collection providing the isolation of strains with definite specificity of the restriction-modification system. Different clones of methicillin resistant S. aureus were found to be circulating on the territory of the CIS.
- Published
- 1994
22. [Conjugative plasmid transfer in Staphylococcus aureus?].
- Author
-
Zueva VS, Klitsunova NV, Dmitrenko OA, and Nesterenko LN
- Subjects
- Collodion, Culture Media, Drug Resistance, Microbial genetics, Filtration instrumentation, Lysogeny genetics, Microscopy, Electron, Staphylococcus aureus ultrastructure, Conjugation, Genetic genetics, Plasmids genetics, Staphylococcus aureus genetics
- Abstract
The microbiological and electron-microscopic study of the transfer of conjugative (pG873) and nonconjugative (rms7 and pE994) plasmids in two systems, on nitrocellulose filters and in a fluid culture medium, was carried out. In both systems the low frequency of the transfer of plasmid pG873 or the absence of the transfer of plasmids rms7 and pE994 were observed when nonlysogenic recipients were used for crossing. The presence of prophage in recipient cells increased the rate of the detection of gentamicin-sensitive transconjugants 100-fold and provided to reveal the transfer rms7 and pE994 plasmids. The electron-microscopic study of specimens with lysogenic recipients revealed a picture which can be interpreted as the fusion of two cells. Such picture was not observed in crossings with a nonlysogenic recipient and in preparations obtained from separate donor and recipient cultures.
- Published
- 1994
23. [A new collection of phages for typing methicillin resistant Staphylococcus aureus].
- Author
-
Zueva VS, Dmitrenko OA, Gladkova KK, and Zueva EA
- Subjects
- Burns microbiology, Female, Humans, Pregnancy, Pregnancy Complications microbiology, Staphylococcus Phages, Staphylococcus aureus drug effects, Staphylococcus aureus isolation & purification, Wounds and Injuries microbiology, Bacteriophage Typing methods, Methicillin Resistance, Staphylococcus aureus classification
- Abstract
A new collection of phages for typing methicillin-resistant S. aureus (MRSA) is proposed. The collection includes phage 85 (modified by the MRSA strain), capable of selecting strains with the similar specificity of the restriction-modification system, and 9 MRSA-induced phages. The latter differentiate MRSA strains according to the specificity of prophages present in bacterial cells. The use of this phage collection has permitted the typing of MRSA strains insensitive to the phages of the international collection. Among these cultures an epidemic strain has been detected and the source of its spread in the burn center has been established.
- Published
- 1994
24. [Phage-mediated conjugative transfer of plasmids in Staphylococcus aureus].
- Author
-
Zueva VS, Nesterenko LN, and Dmitrenko OA
- Subjects
- Calcium Chloride pharmacology, Culture Media, In Vitro Techniques, Plasmids drug effects, Staphylococcus Phages drug effects, Staphylococcus aureus drug effects, Staphylococcus aureus genetics, Stimulation, Chemical, Conjugation, Genetic physiology, Plasmids genetics, Staphylococcus Phages genetics, Transduction, Genetic genetics
- Abstract
It was shown possible to transfer nonconjugative plasmids during joint cultivation of the donor and recipient cells by transduction and phage-mediated conjugation. In the latter case it was necessary that the phage in the medium was free and the prophage was present in the recipient cells. Differences in the regularities of the transfer of the nonconjugative plasmids mobilized by the conjugative plasmid or phage were observed.
- Published
- 1992
25. [Effect of prophages on transfer frequency of conjugative plasmid G 873].
- Author
-
Zueva VS, Nesterenko LN, Dmitrenko OA, and Witte W
- Subjects
- Animals, Calcium Chloride pharmacology, Culture Media, Humans, In Vitro Techniques, Lysogeny drug effects, Lysogeny genetics, Plasmids genetics, R Factors drug effects, R Factors genetics, Rabbits, Staphylococcus Phages drug effects, Staphylococcus aureus drug effects, Transduction, Genetic drug effects, Conjugation, Genetic physiology, Methicillin pharmacology, Methicillin Resistance genetics, Staphylococcus Phages genetics, Staphylococcus aureus genetics, Transduction, Genetic genetics
- Abstract
Transfer of the conjugative plasmid G873 on filters and mixed cultivation of the donor and recipient cells in liquid media is described. In the both systems the use of the lysogenic recipient cells (phages of serogroups B and F) in the crossings increased mor than 100-fold the frequency of plasmid transfer. The conjugative transfer of the plasmid in the mixed cultivation system was proved. The conjugative transfer required the presence (while not obligatory) of calcium chloride and was restricted by the serum factors.
- Published
- 1991
26. A new approach to establishing the set of phages for typing methicillin-resistant Staphylococcus aureus.
- Author
-
Akatov AK, Zueva VS, and Dmitrenko OA
- Subjects
- Electrophoresis, Humans, Staphylococcus aureus drug effects, Bacteriophage Typing methods, Methicillin Resistance, Staphylococcus Phages, Staphylococcus aureus classification
- Abstract
A new approach to using experimental phages for typing methicillin-resistant S. aureus (MRSA) non-sensitive to the phages of International Basic Set (IBS) is described. The collection Includes phage 85, modified on a culture of MRSA, and 5 phages induced from MRSA strains isolated in clinics of Moscow in 1975-76. Firstly, the modified phage selects cultures according to the specific character of its restriction-modification system, then the induced phages differentiate the selected strains into 5 groups (1, 2, 3, 4, 5) based on the specificity of the prophages they contain. Group 1 strains can further be differentiated into 5 subgroups (A, B, C, D, E) by additional phages. Forty-one MRSA strains isolated in 1987-90 in various hospitals of Moscow showing no sensitivity to IBS phages, were lysed by the modified phage, 15 of them belonging to Group 2 and isolated in the traumatological hospital, 26 belonging to Group 1 and were circulating in the burn center. Twenty-three strains of Group 1 appertain to subgroup 1B and were isolated over a 4-year period from the burned surface of patients and from the throat of a medical staff carrier.
- Published
- 1991
- Full Text
- View/download PDF
27. [The mechanism of the resistance of methicillin-resistant Staphylococcus aureus to phages from the International Collection].
- Author
-
Zueva VS, Dmitrenko OA, Krupina EA, Belikov NG, and Nesterenko LN
- Subjects
- Bacteriophage Typing methods, Cross Infection microbiology, Humans, Staphylococcal Infections microbiology, Staphylococcus Phages physiology, Staphylococcus aureus isolation & purification, Virus Replication, Methicillin antagonists & inhibitors, Penicillin Resistance, Staphylococcus aureus classification
- Abstract
The resistance of methicillin-resistant staphylococci to phage 85 is due to the presence of a certain system restriction modification in microbial cells. The loss of the capacity for restricting phage DNA by the cell as the consequence of the loss of the mec determinant is not accompanied by the loss of its capacity for modifying phage DNA.
- Published
- 1990
28. [Effect of interferon type I on staphylococcal persistence and indices of body immunoreactivity].
- Author
-
Spivak NIa, Lisovenko VG, Zueva VS, Dmitrenko OA, and Beliaev DL
- Subjects
- Animals, Chick Embryo, Hypersensitivity, Delayed immunology, Hypersensitivity, Delayed microbiology, Immunity, Interferon Type I isolation & purification, Kidney microbiology, Mice, Mice, Inbred CBA, Mice, Inbred Strains, Staphylococcal Infections microbiology, Staphylococcal Infections mortality, Staphylococcus isolation & purification, Time Factors, Interferon Type I immunology, Staphylococcal Infections immunology
- Abstract
After the subcutaneous injection of type I (alpha) interferon into mice their survival rate in staphylococcal infection greatly increased. At the same time duration of staphylococcal persistence in these animals and the number of persisting staphylococci were found to decrease. After the injection of interferon the splenocytes of the treated animals showed a higher capacity for interferon production. During the whole experiment the characteristics of delayed hypersensitivity in these animals showed a tendency towards normalization in comparison with those in infected mice receiving no interferon.
- Published
- 1984
29. [Antistaphylococcal activity in interferon preparations].
- Author
-
Kuznetsov VP, Zueva VS, Dmitrenko OA, and Beliaev DL
- Subjects
- Animals, Humans, Immunization, Immunoglobulins immunology, Immunoglobulins isolation & purification, Interferon Type I antagonists & inhibitors, Nasal Mucosa microbiology, Sheep immunology, Time Factors, Interferon Type I pharmacology, Staphylococcus aureus drug effects
- Abstract
Antistaphylococcal bacteriostatic and bactericidal activity was detected in the preparations of human leucocytic interferon of the native type. Such an activity could not be neutralized by the interferon antibodies. However, it was usually lost during the process of interferon purification. It is concluded that this antistaphylococcal activity is not related to interferon and is due to other factors which can be isolated with induced leucocytes.
- Published
- 1982
30. [Dynamics od streptomycin sensitivity in staphylococci from the nasal mucosa of patients after discharge from a surgical hospital].
- Author
-
Dmitrenko OA and Zueva VS
- Subjects
- Humans, Microbial Sensitivity Tests, Patient Discharge, Staphylococcus isolation & purification, Time Factors, Nasal Mucosa microbiology, Staphylococcus drug effects, Streptomycin pharmacology
- Published
- 1979
31. [Drug resistance stability in staphylococci under different conditions. The mechanism of the recovery of streptomycin sensitivity of a staphylococcal population in patients 7 months after hospital discharge].
- Author
-
Zueva VS and Dmitrenko OA
- Subjects
- Drug Resistance, Microbial, Humans, Nasal Mucosa microbiology, Staphylococcal Infections microbiology, Time Factors, Staphylococcus drug effects, Streptomycin pharmacology
- Abstract
A method for analysis of antibiotic sensitivity restoration in staphylococcal populations in humans is described. The mechanism of streptomycin sensitivity restoration in staphylococcal populations on the nasal mucosa of 59 patients 7 months after discharging from surgical stationary was studied. Quantitative estimates for loss of superinfecting streptomycin-resistant staphylococci, initial streptomycin-resistant staphylococci and resistance determinants by the initial streptomycin-resistant staphylococci are presented.
- Published
- 1978
32. [Methicillin-resistant Staphylococci].
- Author
-
Zueva VS, Dmitrenko OA, Akatov AK, Zueva EA, and Pasynkova LN
- Subjects
- Bacteriophage Typing, Cross Infection microbiology, Disease Outbreaks, Humans, Plasmids, Staphylococcal Infections microbiology, Staphylococcus classification, Staphylococcus genetics, Methicillin antagonists & inhibitors, Penicillin Resistance, Staphylococcus drug effects
- Published
- 1988
33. [Experimental phages for differentiating nontypable methicillin-resistant Staphylococcus aureus].
- Author
-
Zueva EA, Dmitrenko OA, and Akatov AK
- Subjects
- Genetic Variation, Lysogeny, Staphylococcus Phages isolation & purification, Virology methods, Virus Activation, Bacteriophage Typing methods, Methicillin antagonists & inhibitors, Penicillin Resistance, Staphylococcus aureus classification
- Abstract
The possibility of using phages, isolated from the lysogenic cultures of methicillin-resistant staphylococci and modified in methicillin-resistant cultures of phage 85, for the differentiation of nontypable staphylococcal strains has been studied. The variants of phage 85 cannot be used for the determination of differences between the strains of methicillin-resistant cultures; they are not suited for typing. For this purpose the collection of phages isolated from lysogenic methicillin-resistant cultures should be used.
- Published
- 1988
34. [In vivo stability of various drug resistance plasmids in staphylococci].
- Author
-
Zueva VS, Linevich IuG, Witte W, and Dmitrenko OA
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Drug Resistance, Microbial, Staphylococcus aureus drug effects, Plasmids drug effects, R Factors drug effects, Staphylococcus aureus genetics
- Published
- 1978
35. [Stability of drug resistance in staphylococci under various conditions and the effect on it of prolonged culture storage on artificial nutrient media].
- Author
-
Zueva VS, Dmitrenko OA, and Linevich IuG
- Subjects
- Anti-Bacterial Agents pharmacology, Drug Resistance, Microbial, Microbial Sensitivity Tests, Time Factors, Culture Media pharmacology, Preservation, Biological, Staphylococcus drug effects
- Abstract
The literature and experimental data on stability of resistance ot benzylpenicillin, streptomycin, tetracycline, chloramphenicol, erythromycin, kanamycin and methicillin in staphylococci stored for prolonged periods of time on artificial nutrient media were systematized. When the staphylococcal cultures were not in contact iwth the antibiotics for 5 to 18 months, sensitive cells to separate drugs were detected in 51.0--67.4 per cent of the strains. The number of such cells in the populations of some strains ranged from 1 to 100.0 per cent. The effect of the resistance multiplicity on the stability of resistance to benzylpenicillin, tetracycline, chloramphenicol and erythromycin was not observed.
- Published
- 1978
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