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The article presents the results of a probabilistic analysis of resources in agricultural processes. The authors performed a comparative analysis of the effectiveness of using the GERT method for assessing the probabilistic-time characteristics of processes with simulation modeling in the Anylogic environment. For the study, the process of “Approval of an application for the purchase of agricultural products” is described. This process is first presented in the ARIS eEPC notation, and then it is translated into the GERT network. Based on the obtained model, the process completion time was predicted. The mathematical expectation and variance of the process execution were calculated. Based on the results obtained, conclusions were drawn regarding labor intensity and accuracy. It is shown that the GERT network and the simulation model are close in accuracy. However, for the given task, the GERT method is a less labor-intensive and more accurate method. This study is important for managing and optimizing processes in agricultural enterprises to improve the efficiency of decision making.

Background: Congenital hypothyroidism screening traditionally relies on detecting elevated thyroid-stimulating hormone levels, yet this approach may not detect a specific type of congenital hypothyroidism caused by iodotyrosine dehalogenase-1 (Dehal1) deficiency. The deficiency of this enzyme prevents the deiodination of mono-iodotyrosine (MIT) and di-iodotyrosine (DIT) in the process of iodine recycling. This underscores the potential use of iodotyrosine or its metabolites as non-invasive urinary biomarkers for early diagnosis of congenital hypothyroidism. However, the urinary metabolites of MIT/DIT have not yet been discovered. Thus, this study aimed to identify the urinary metabolites of iodotyrosine in experimental models., Method: Gas chromatography mass spectrometry was used to identify the urinary metabolites of iodotyrosine following intraperitoneal injection of MIT in rats. An isotope dilution mass spectrometric assay was developed for assessment of identified metabolites. Urine samples from Dehal1 knockout mice were used to confirm the results., Results: We identified novel iodotyrosine metabolites, 3-iodo-4-hydroxyphenylacetic acid (IHPA), and 3,5-diiodo-4-hydroxyphenylacetic acid (Di-IHPA) as the primary urinary metabolites of MIT and DIT respectively. The concentrations of urinary IHPA and Di-IHPA were significantly higher in Dehal1 knockout mice., Conclusion: Our findings suggest that IHPA is detected in larger quantities and may hold more clinical significance than previously identified biomarkers like MIT and DIT, making it a promising candidate for diagnosing congenital hypothyroidism or other conditions associated with iodine recycling inhibition., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Background: Multiple sclerosis is a chronic disease of the central nervous system characterized by inflammation, neurodegeneration, and failure of the central nervous system's repair mechanisms. The role of infectious agents against the background of genetic predisposition is currently considered a possible pathogenesis factor of this disease., Case Presentation: We report the case of a 52-year-old white (Russian) female musician with 15-year history of relapsing-remitting multiple sclerosis who had repeatedly received conventional therapy without much benefit. In 2017, she was admitted to the outpatient department of the Institute of Virology, where she was treated with erythromycin and acyclovir (tablet forms), which were not applied in the traditional way but through the "device for transfer of information from the drug to the human body." The received effect led to suppression of the disease activity, a significant reduction in the symptoms of the disease, prevention of further increase in neurological manifestations of the disease, and improvement in the dynamics of the manifestation of the disease according to brain magnetic resonance imaging., Conclusion: The described case report is innovative and presents for the first time the results of a noninvasive approach to the treatment of a patient with multiple sclerosis in whom information about various medications was introduced into different parts of the body, including the brain. The results obtained may indicate a possible role of infectious agents in the genesis of multiple sclerosis. It indicates a potential impact on them by using a "device for transfer of information from the drug to the human body." The study was conducted in accordance with the principles of the Declaration of Helsinki. It was approved by the Institutional Review Board of the Research Institute of Virology of Uzbekistan (no. 12/8-1500, 1/3/2017)., Competing Interests: Declarations. Consent for publication: Written informed consent was obtained from the patient's legal guardian to publish this case report of a 59-year-old female patient and any accompanying images. A copy of the written consent is available for review by the Editor-in- Chief of this journal. Competing interests: The author declares no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© 2024. The Author(s).)