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Abstract: The activity of glycolytic enzymes and the expression levels of myosin RNA was monitored in the white muscle of juvenile spotted wolffish (Anarhichas minor) reared under different temperature regimes. [...]

Abstract Background The insulin signaling pathway (ISP) has a key role in major physiological events like carbohydrate metabolism and growth regulation. The ISP has been well described in vertebrates and in a few invertebrate model organisms but remains largely unexplored in non-model invertebrates. This study is the first detailed genomic study of this pathway in a crustacean species, Daphnia pulex. Results The Daphnia pulex draft genome sequence assembly was scanned for major components of the ISP with a special attention to the insulin-like receptor. Twenty three putative genes are reported. The pathway appears to be generally well conserved as genes found in other invertebrates are present. Major findings include a lower number of insulin-like peptides in Daphnia as compared to other invertebrates and the presence of multiple insulin-like receptors (InR), with four genes as opposed to a single one in other invertebrates. Genes encoding for the Dappu_InR are likely the result of three duplication events and bear some unusual features. Dappu_InR-4 has undergone extensive evolutionary divergence and lacks the conserved site of the catalytic domain of the receptor tyrosine kinase. Dappu_InR-1 has a large insert and lacks the transmembranal domain in the β-subunit. This domain is also absent in Dappu_InR-3. Dappu_InR-2 is characterized by the absence of the cystein-rich region. Real-time q-PCR confirmed the expression of all four receptors. EST analyses of cDNA libraries revealed that the four receptors were differently expressed under various conditions. Conclusions Duplications of the insulin receptor genes might represent an important evolutionary innovation in Daphnia as they are known to exhibit extensive phenotypic plasticity in body size and in the size of defensive structures in response to predation.

The article discusses a research which described a novel salmon gill poxvirus (SGPV) variant among Atlantic salmon in Eastern Canada. It attempts to isolate and identify the variant. It discusses the results of disease testing, quantitative polymerase chain reaction and transmission electron microscopy.

This is the first report of early-life developmental characteristics (coloration pattern, growth and survival) and genetic identification of the wolffish interspecific hybrid between Anarhichas minor and A. lupus, both endangered species in Canadian coastal water and of interest for cold-water aquaculture diversification. A first growth trial at 8 °C featuring pure strains A. minor and the hybrid A. minor × A. lupus in triplicates was conducted in 2006 during the period from 0 to 140 days post-hatch. A second growth trial was runned in 2007 featuring A. minor, A. lupus and the reciprocal hybrids A. minor × A. lupus and A. lupus × A. minor. Egg development indicators and early-hatching characteristics are reported. [ABSTRACT FROM AUTHOR]

Rainbow smelt is a small fish that accumulates glycerol in winter as a cryoprotectant when the animal is in seawater. Glycerol is synthesized in liver from different substrates that all lead to the formation of glycerol-3-phosphate (G3P). This study assesses whether glycerol is produced by a direct dephosphorylation of G3P by a phosphatase (G3Pase) or by a cycling through the glycerolipid pool followed by lipolysis. Foremost, concentrations of on-board glycerolipids and activity of G3Pase and of enzymes involved in lipid metabolism were measured in smelt liver over the glycerol cycle. Concentrations of on-board glycerolipids did not change over the cycle and were too low to significantly contribute directly to glycerol production but activities of enzymes involved in both potential pathways were up-regulated at the onset of glycerol accumulation. A second experiment conducted with isolated hepatic cells producing glycerol showed 1) that on-board glycerolipids were not sufficient to produce the glycerol released even though phospholipids could account for up to 17% of it, 2) that carbon cycling through the glycerolipid pool was not involved as glycerol was produced at similar rates following inhibition of this pathway, and 3) that G3Pase activity measured was sufficient to allow the synthesis of glycerol at the rate observed. These results are the first to clearly support G3Pase as the metabolic step leading to glycerol production in rainbow smelt and the first to provide strong support for a G3Pase in any animal species.

Cephalopods have relatively high rates of protein synthesis compared to rates of protein degradation, along with minimal carbohydrate and lipid reserves. During food deprivation on board protein is catabolized as a metabolic fuel. The aim of the current study was to assess whether biochemical indices of protein synthesis and proteolytic mechanisms were altered in cuttlefish, Sepia officinalis, starved for 7 days. In mantle muscle, food deprivation is associated with a decrease in protein synthesis, as indicated by a decrease in the total RNA level and dephosphorylation of key signaling molecules, such as the eukaryote binding protein, 4E-BP1 (regulator of translation) and Akt. The ubiquitination-proteasome system (UPS) is activated as shown by an increase in the levels of proteasome β-subunit mRNA, polyubiquitinated protein, and polyubiquitin mRNA. As well, cathepsin activity levels are increased, suggesting increased proteolysis through the lysosomal pathway. Together, these mechanisms could supply amino acids as metabolic fuels. In gill, the situation is quite different. It appears that during the first stages of starvation, both protein synthesis and protein degradation are enhanced in gill. This is based upon increased phosphorylation of 4E-BP1 and enhanced levels of UPS indicators, especially 20S proteasome activity and polyubiquitin mRNA. It is proposed that an increased protein turnover is related to gill remodeling perhaps to retain essential hemolymph-borne compounds.

Rainbow smelt accumulate high amounts of glycerol in winter. In smelt, there is a predictable profile of plasma glycerol levels that starts to increase in November (<5 μmol/ml), peaks in mid-February (>200 μmol/ml), and thereafter decreases to reach the initial levels in the beginning of May. The aim of this study was to investigate the respective role of the two main mechanisms that might be involved in glycerol clearance from mid-February: 1) breakdown of glycerol to glycerol-3-phosphate through the action of the glycerol kinase (GK) and 2) direct loss toward the environment. Over the entire glycerol cycle, loss to water represents a daily loss of ∼10% of the total glycerol content of fish. GK activities were very low in all tissues investigated and likely have a minor quantitative role in the glycerol cycle. These results suggest that glycerol levels are dictated by the rate of glycerol synthesis (accelerated and deactivated during the accumulation and decrease stages, respectively). Although not important in glycerol clearance, GK in liver might have an important metabolic function for other purposes, such as gluconeogenesis, as evidenced by the significant increase of activity at the end of the cycle.