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30 results on '"Distel, Robert J."'

2. Recurrent ubiquitin B silencing in gynecological cancers establishes dependence on ubiquitin C

Author

Kedves, Alexia T., Gleim, Scott, Liang, Xiaoyou, Bonal, Dennis M., Harbinski, Fred, Sigoillot, Frederic, Sanghavi, Sneha, Benander, Christina, George, Elizabeth, Gokhale, Prafulla C., Nguyen, Quang-De, Kirschmeier, Paul T., Distel, Robert J., Jenkins, Jeremy, Goldberg, Michael S., and Forrester, William C.

Subjects
Ubiquitin -- Research, Gene silencing -- Research, Ovarian cancer -- Genetic aspects -- Prognosis -- Research, Health care industry
Abstract

Transcriptional repression of ubiquitin B (UBB) is a cancer-subtype-specific alteration that occurs in a substantial population of patients with cancers of the female reproductive tract. UBB is 1 of 2 genes encoding for ubiquitin as a polyprotein consisting of multiple copies of ubiquitin monomers. Silencing of UBB reduces cellular UBB levels and results in an exquisite dependence on ubiquitin C (UBC), the second polyubiquitin gene. UBB is repressed in approximately 30% of high-grade serous ovarian cancer (HGSOC) patients and is a recurrent lesion in uterine carcinosarcoma and endometrial carcinoma. We identified ovarian tumor cell lines that retain UBB in a repressed state, used these cell lines to establish orthotopic ovarian tumors, and found that inducible expression of a UBC-targeting shRNA led to tumor regression, and substantial long-term survival benefit. Thus, we describe a recurrent cancer-specific lesion at the level of ubiquitin production. Moreover, these observations reveal the prognostic value of UBB repression and establish UBC as a promising therapeutic target for ovarian cancer patients with recurrent UBB silencing., Introduction Target discovery in oncology has focused predominantly on 2 basic principles: oncogene addiction (1) and synthetic lethality (2). Identifying cancer-specific gain-of-function alterations in oncogenes such as ABL, BRAF, EGFR, [...]

Published
2017
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8. RATA: A method for high-throughput identification of RNA bound transcription factors

Author

Schmidt, Karyn, Buquicchio, Frank, Carroll, Johanna S., Distel, Robert J., Novina, Carl D., Schmidt, Karyn, Buquicchio, Frank, Carroll, Johanna S., Distel, Robert J., and Novina, Carl D.

Abstract

Long non-coding RNAs (lncRNAs) regulate critical cellular processes and their dysregulation contributes to multiple diseases. Although only a few lncRNAs have defined mechanisms, many of these characterized lncRNAs interact with transcription factors to regulate gene expression, suggesting a common mechanism of action. Identifying RNA-boundtranscription factors is especially challenging due to inefficient RNA immunoprecipitation and low abundance of many transcription factors. Here we describe a highly sensitive, user-friendly, and inexpensive technique called RATA (RNA-associated transcription factor array), which utilizes a MS2-aptamer pulldown strategy coupled with transcription factor activation arrays for identification of transcription factors associated with a nuclear RNA of interest. RATA requires only ~5 million cells and standard molecular biology reagents for multiplexed identification of up to 96 transcription factors in 2–3 d. Thus, RATA offers significant advantages over other technologies for analysis of RNA-transcription factor interactions.

Published
2017

10. Accelerating research into clinical setting

Author

Kuo, Winston Patrick and Distel, Robert J.

Subjects
Biotechnology -- Usage, Biotechnology laboratories -- Services, Biotechnology industry, Business
Abstract

Harvard University's Laboratory for Innovative Transnational Technologies (LIIT) seeks different technologies for use by in biotechnology research. It will perform comparisons between competing technologies before validating them. The laboratory will focus on hosting technologies that can benefit local researchers.

Published
2008

11. Alternative Methods for Characterization of Extracellular Vesicles

Author

Momen-Heravi, Fatemeh, primary, Balaj, Leonora, additional, Alian, Sara, additional, Tigges, John, additional, Toxavidis, Vasilis, additional, Ericsson, Maria, additional, Distel, Robert J., additional, Ivanov, Alexander R., additional, Skog, Johan, additional, and Kuo, Winston Patrick, additional

Published
2012
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13. A Rapid and Sensitive Enzymatic Method for Epidermal Growth Factor Receptor Mutation Screening

Author

Jänne, Pasi A., primary, Borras, Ana M., additional, Kuang, Yanan, additional, Rogers, Andrew M., additional, Joshi, Victoria A., additional, Liyanage, Hema, additional, Lindeman, Neal, additional, Lee, Jeffrey C., additional, Halmos, Balazs, additional, Maher, Elizabeth A., additional, Distel, Robert J., additional, Meyerson, Matthew, additional, and Johnson, Bruce E., additional

Published
2006
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15. The lncRNA SLNCR1Mediates Melanoma Invasion through a Conserved SRA1-like Region

Author

Schmidt, Karyn, Joyce, Cailin E., Buquicchio, Frank, Brown, Adam, Ritz, Justin, Distel, Robert J., Yoon, Charles H., and Novina, Carl D.

Abstract

Long non-coding RNAs (lncRNAs) have been implicated in numerous physiological processes and diseases, most notably cancers. However, little is known about the mechanism of many functional lncRNAs. We identified an abundantly expressed lncRNA associated with decreased melanoma patient survival. Increased expression of this lncRNA, SLNCR1, mediates melanoma invasion through a highly conserved sequence similar to that of the lncRNA SRA1. Using a sensitive technique we term RATA (RNA-associated transcription factor array), we show that the brain-specific homeobox protein 3a (Brn3a) and the androgen receptor (AR) bind within and adjacent to SLNCR1’s conserved region, respectively. SLNCR1, AR, and Brn3a are specifically required for transcriptional activation of matrix metalloproteinase 9 (MMP9) and increased melanoma invasion. Our observations directly link AR to melanoma invasion, possibly explaining why males experience more melanoma metastases and have an overall lower survival in comparison to females.

Published
2016
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16. Haploid expression of a mouse testis a-Tubulin gene

Author

Distel, Robert J., Kleene, Kenneth C., and Hecht, Norman B.

Subjects
Biological research -- Research, Biology, Experimental -- Research, DNA -- Research, Spermatogenesis in animals -- Research, Genetic research -- Research, Reproduction -- Research, Science and technology, Research
Abstract

Spermatogenesis in mammals is a continuous process in which mitotic proliferation of spermatogonia is followed by meiosis and differentiation of haploid spermatids into mature spermatozoa (1). The major morphological changes [...]

Published
1984

30. A new device for rapid isolation by size and characterization of rare circulating tumor cells.

Author

Desitter I, Guerrouahen BS, Benali-Furet N, Wechsler J, Jänne PA, Kuang Y, Yanagita M, Wang L, Berkowitz JA, Distel RJ, and Cayre YE

Subjects
Carcinoma, Non-Small-Cell Lung blood, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung pathology, Cell Line, Tumor, Cell Separation instrumentation, Cell Separation methods, Cell Size, Colorectal Neoplasms blood, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, ErbB Receptors genetics, Exons, Filtration instrumentation, Filtration methods, Gene Deletion, HT29 Cells, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Lung Neoplasms blood, Lung Neoplasms genetics, Lung Neoplasms pathology, Reverse Transcriptase Polymerase Chain Reaction, Cytological Techniques instrumentation, Cytological Techniques methods, Neoplastic Cells, Circulating pathology
Abstract

Background: Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells., Materials and Methods: The ScreenCell® devices are single-use and low-cost innovative devices that use a filter to isolate and sort tumor cells by size., Results: The ScreenCell® Cyto device is able to isolate rare, fixed, tumor cells, with a high recovery rate. Cells are well preserved morphologically. Immunocytochemistry and FISH assays can be performed directly on the filter. The ScreenCell® CC device allows isolation of live cells able to grow in culture. High quality genetic materials can be obtained directly from tumor cells isolated on the ScreenCell® MB device filter., Conclusion: Due to their reduced size, versatility, and capacity to isolate CTCs within minutes, the ScreenCell® devices may be able to simplify and improve non-invasive access to tumor cells.

Published
2011

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