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2. How often do they have sex? A comparative analysis of the population structure of seven eukaryotic microbial pathogens.

Author

Tomasini, Nicolás, Lauthier, Juan José, Ayala, Francisco José, Tibayrenc, Michel, and Diosque, Patricio

Subjects
Humans, Bacteria, Bacterial Infections, Phylogeny, Recombination, Genetic, Genotype, Genes, Bacterial, Genetic Variation, Multilocus Sequence Typing, General Science & Technology
Abstract

The model of predominant clonal evolution (PCE) proposed for micropathogens does not state that genetic exchange is totally absent, but rather, that it is too rare to break the prevalent PCE pattern. However, the actual impact of this "residual" genetic exchange should be evaluated. Multilocus Sequence Typing (MLST) is an excellent tool to explore the problem. Here, we compared online available MLST datasets for seven eukaryotic microbial pathogens: Trypanosoma cruzi, the Fusarium solani complex, Aspergillus fumigatus, Blastocystis subtype 3, the Leishmania donovani complex, Candida albicans and Candida glabrata. We first analyzed phylogenetic relationships among genotypes within each dataset. Then, we examined different measures of branch support and incongruence among loci as signs of genetic structure and levels of past recombination. The analyses allow us to identify three types of genetic structure. The first was characterized by trees with well-supported branches and low levels of incongruence suggesting well-structured populations and PCE. This was the case for the T. cruzi and F. solani datasets. The second genetic structure, represented by Blastocystis spp., A. fumigatus and the L. donovani complex datasets, showed trees with weakly-supported branches but low levels of incongruence among loci, whereby genetic structuration was not clearly defined by MLST. Finally, trees showing weakly-supported branches and high levels of incongruence among loci were observed for Candida species, suggesting that genetic exchange has a higher evolutionary impact in these mainly clonal yeast species. Furthermore, simulations showed that MLST may fail to show right clustering in population datasets even in the absence of genetic exchange. In conclusion, these results make it possible to infer variable impacts of genetic exchange in populations of predominantly clonal micro-pathogens. Moreover, our results reveal different problems of MLST to determine the genetic structure in these organisms that should be considered.

Published
2014

4. Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes

Author

Cura, Carolina I., Mejía-Jaramillo, Ana M., Duffy, Tomás, Burgos, Juan M., Rodriguero, Marcela, Cardinal, Marta V., Kjos, Sonia, Gurgel-Gonçalves, Rodrigo, Blanchet, Denis, De Pablos, Luis M., Tomasini, Nicolás, da Silva, Alexandre, Russomando, Graciela, Cuba, Cesar A. Cuba, Aznar, Christine, Abate, Teresa, Levin, Mariano J., Osuna, Antonio, Gürtler, Ricardo E., Diosque, Patricio, Solari, Aldo, Triana-Chávez, Omar, and Schijman, Alejandro G.

Published
2010
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7. List of Contributors

Author

Aguileta, Gabriela, primary, Alout, Haoues, additional, Baquero, Fernando, additional, Barnabé, Christian, additional, Basset, Patrick, additional, Behr, Marcel A., additional, Biron, David G., additional, Blanc, Dominique S., additional, Byrnes, Edmond J., additional, Cochran, Gregory, additional, Cohan, Frederick M., additional, Comas, Iñaki, additional, Cornel, Anthony J., additional, Crandall, Keith A., additional, Delaporte, Eric, additional, Carlos Dib, Juan, additional, Diosque, Patricio, additional, Djogbénou, Luc, additional, Dorn, Patricia L., additional, Dujardin, Jean-Pierre, additional, Etienne, Lucie, additional, Feil, Edward J., additional, Field, Hugh J., additional, C. Fisher, Matthew, additional, Fouchier, Ron A.M., additional, Gagneux, Sébastien, additional, Carlos Galán, Juan, additional, Gibson, Wendy, additional, van der Giezen, Mark, additional, Gilabert, Aude, additional, Giraud, Tatiana, additional, Gladieux, Pierre, additional, González-Candelas, Fernando, additional, Hanage, William P., additional, Harpending, Henry, additional, Heitman, Joseph, additional, Holzmuller, Philippe, additional, Kopac, Sarah, additional, Koskella, Britt, additional, Krafsur, Elliot S., additional, Labbé, Pierrick, additional, Lanzaro, Gregory C., additional, Lewis, Michael D., additional, Liu, Yu-Tsueng, additional, Luis Martínez, José, additional, Mahungu, Tabitha, additional, de Meeûs, Thierry, additional, Miles, Michael, additional, Missé, Dorothée, additional, Morgan, Andrew D., additional, Morrison, David A., additional, Munster, Vincent J., additional, Nedelkov, Dobrin, additional, Noireau, François, additional, Owen, Andrew, additional, Pasteur, Nicole, additional, Paszkiewicz, Konrad H., additional, Peeters, Martine, additional, Pérez-Losada, Marcos, additional, Polley, Spencer D., additional, Porter, Megan L., additional, Prugnolle, Franck, additional, Robinson, D. Ashley, additional, Shi, Zhengli, additional, Sutherland, Colin J., additional, Telleria, Jenny, additional, Thomas, Jonathan C., additional, Tibayrenc, Michel, additional, Van Regenmortel, Marc H.V., additional, Vere Hodge, Anthony, additional, Verhagen, Josanne H., additional, Vernet, Guy, additional, Viscidi, Raphael P., additional, Wang, Lin-Fa, additional, Weill, Mylène, additional, Wirth, Thierry, additional, and Zanetti, Giorgio, additional

Published
2011
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10. High performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens

Author

Bracamonte, María E., primary, Álvarez, Agustín Moya, additional, Sosa, Andrea M., additional, Hoyos, Carlos L., additional, Lauthier, Juan J., additional, Cajal, Silvana P., additional, Juarez, Marisa, additional, Uncos, Renato E., additional, Sánchez-Valdéz, Fernando J., additional, Acuña, Leonardo, additional, Diosque, Patricio, additional, Basombrío, Miguel A., additional, Nasser, Julio R., additional, Hashiguchi, Yoshihisa, additional, Korenaga, Masataka, additional, Barroso, Paola A., additional, and Marco, Jorge D., additional

Published
2020
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12. TcTASV Antigens of Trypanosoma cruzi: Utility for Diagnosis and High Accuracy as Biomarkers of Treatment Efficacy in Pediatric Patients

Author

Floridia-Yapur, Noelia, primary, Monje-Rumi, Mercedes, additional, Ragone, Paula, additional, Lauthier, Juan J., additional, Tomasini, Nicolás, additional, Alberti D’Amato, Anahí, additional, Diosque, Patricio, additional, Cimino, Rubén, additional, Gil, José F., additional, Sanchez, Daniel O., additional, Nasser, Julio R., additional, and Tekiel, Valeria, additional

Published
2019
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13. Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in trypanosoma cruzi.

Author

588790, Yeo, Matthew, Mauricio, Isabel L., Messenger, Louisa A. M., Lewis, Michael D., Llewellyn, Martin S., Acosta, Nidia, Bhattacharyya, Tapan, Diosque, Patricio, Carrasco, Hernan J., Miles, Michael A., 588790, Yeo, Matthew, Mauricio, Isabel L., Messenger, Louisa A. M., Lewis, Michael D., Llewellyn, Martin S., Acosta, Nidia, Bhattacharyya, Tapan, Diosque, Patricio, Carrasco, Hernan J., and Miles, Michael A.

Abstract

Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed.

Published
2019

17. Additional file 1: Figure S1. of Effects of IFN-γ coding plasmid supplementation in the immune response and protection elicited by Trypanosoma cruzi attenuated parasites

Author

Brandán, Cecilia Pérez, Mesías, Andrea, Parodi, Cecilia, Cimino, Rubén, Brandán, Carolina Pérez, Diosque, Patricio, and Basombrío, Miguel

Abstract

pVXVR-mIFN-γ administration alters the parasite-specific immune response elicited by infection with attenuated parasites by the intramuscular and oral route. Mice (n = 4) were infected (4 weeks apart) with 3 doses of 105 metacyclic trypomatigotes of the attenuated TCC strain, 50 μg of plasmid pVXVR-mIFN-γ or a combination of both by the intramuscular (A-C) or oral (B-D) route. After each infection dose, serum samples were collected for (A-B) T. cruzi specific total IgGs levels and (C-D) serum levels of parasite specific IgG subtypes. (PDF 48 kb)

Published
2017
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22. Seroprevalence of Triatoma virus (Dicistroviridae

Author

Querido, Jailson F B, Echeverría, María G, Marti, Gerardo A, Costa, Rita Medina, Susevich, María L, Rabinovich, Jorge E, Copa, Aydee, Montaño, Nair A, Garcia, Lineth, Cordova, Marisol, Torrico, Faustino, Sánchez-Eugenia, Rubén, Sánchez-Magraner, Lissete, Muñiz-Trabudua, Xabier, López-Marijuan, Ibai, Rozas-Dennis, Gabriela S, Diosque, Patricio, de Castro, Ana M, Robello, Carlos, Rodríguez, Julio S, Altcheh, Jaime, Salazar-Schettino, Paz M, Bucio, Marta I, Espinoza, Bertha, Guérin, Diego M A, Silva, Marcelo Sousa, Instituto de Higiene e Medicina Tropical (IHMT), Global Health and Tropical Medicine (GHTM), and Vector borne diseases and pathogens (VBD)

Subjects
Viral Structural Proteins, Portugal, Research Support, Non-U.S. Gov't, Enzyme-Linked Immunosorbent Assay, Antibodies, Viral, Models, Biological, SDG 3 - Good Health and Well-being, Seroepidemiologic Studies, parasitic diseases, Dicistroviridae, Journal Article, Animals, Humans, Chagas Disease, Triatoma, Americas
Abstract

PMID: 25595198 WOS:000351038800001 BACKGROUND: Chagas disease is caused by Trypanosoma cruzi, and humans acquire the parasite by exposure to contaminated feces from hematophagous insect vectors known as triatomines. Triatoma virus (TrV) is the sole viral pathogen of triatomines, and is transmitted among insects through the fecal-oral route and, as it happens with T. cruzi, the infected insects release the virus when defecating during or after blood uptake. METHODS: In this work, we analysed the occurrence of anti-TrV antibodies in human sera from Chagas disease endemic and non-endemic countries, and developed a mathematical model to estimate the transmission probability of TrV from insects to man, which ranged between 0.00053 and 0.0015. RESULTS: Our results confirm that people with Chagas disease living in Bolivia, Argentina and Mexico have been exposed to TrV, and that TrV is unable to replicate in human hosts. CONCLUSIONS: We presented the first experimental evidence of antibodies against TrV structural proteins in human sera. publishersversion published

Published
2015

23. Epidemiology of American Tegumentary Leishmaniasis andTrypanosoma cruziInfection in the Northwestern Argentina

Author

Hoyos, Carlos L., primary, Cajal, Silvana P., additional, Juarez, Marisa, additional, Marco, Jorge D., additional, Alberti D’Amato, Anahí M., additional, Cayo, Melina, additional, Torrejón, Irma, additional, Cimino, Rubén O., additional, Diosque, Patricio, additional, Krolewiecki, Alejandro J., additional, Nasser, Julio R., additional, and Gil, José F., additional

Published
2016
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24. Seroprevalence of Triatoma virus (Dicistroviridae: Cripaviridae) antibodies in Chagas disease patients

Author

Bioquímica y biología molecular, Biokimika eta biologia molekularra, Querido, Jailson F. B., Echeverría, María G., Martí, Gerardo A., Medina Costa, Rita, Susevich, María L., Rabinovich, Jorge E., Copa, Aydee, Montaño, Nair A., Garcia, Lineth, Cordova, Marisol, Torrico, Faustino, Sánchez Eugenia, Rubén, Sánchez Magraner, Lissete, Muñiz Trabudua, Xabier, López Marijuan, Ibai, Rozas-Dennis, Gabriela S., Diosque, Patricio, De Castro, Ana M., Robello, Carlos, Rodríguez, Julio S., Altcheh, Jaime, Salazar-Schettino, Paz M., Bucio, Marta I., Espinoza, Bertha, Guérin Silva, Diego M. A., Bioquímica y biología molecular, Biokimika eta biologia molekularra, Querido, Jailson F. B., Echeverría, María G., Martí, Gerardo A., Medina Costa, Rita, Susevich, María L., Rabinovich, Jorge E., Copa, Aydee, Montaño, Nair A., Garcia, Lineth, Cordova, Marisol, Torrico, Faustino, Sánchez Eugenia, Rubén, Sánchez Magraner, Lissete, Muñiz Trabudua, Xabier, López Marijuan, Ibai, Rozas-Dennis, Gabriela S., Diosque, Patricio, De Castro, Ana M., Robello, Carlos, Rodríguez, Julio S., Altcheh, Jaime, Salazar-Schettino, Paz M., Bucio, Marta I., Espinoza, Bertha, and Guérin Silva, Diego M. A.

Abstract

Background: Chagas disease is caused by Trypanosoma cruzi, and humans acquire the parasite by exposure to contaminated feces from hematophagous insect vectors known as triatomines. Triatoma virus (TrV) is the sole viral pathogen of triatomines, and is transmitted among insects through the fecal-oral route and, as it happens with T. cruzi, the infected insects release the virus when defecating during or after blood uptake. Methods: In this work, we analysed the occurrence of anti-TrV antibodies in human sera from Chagas disease endemic and non-endemic countries, and developed a mathematical model to estimate the transmission probability of TrV from insects to man, which ranged between 0.00053 and 0.0015. Results: Our results confirm that people with Chagas disease living in Bolivia, Argentina and Mexico have been exposed to TrV, and that TrV is unable to replicate in human hosts. Conclusions: We presented the first experimental evidence of antibodies against TrV structural proteins in human sera.

Published
2015

25. Effects of IFN-γ coding plasmid supplementation in the immune response and protection elicited by Trypanosoma cruzi attenuated parasites.

Author

Brandán, Cecilia Pérez, Mesías, Andrea C., Parodi, Cecilia, Cimino, Rubén O., Brandán, Carolina Pérez, Diosque, Patricio, Basombrío, Miguel Ángel, Pérez Brandán, Cecilia, and Pérez Brandán, Carolina

Subjects
TRYPANOSOMA cruzi, IMMUNE response, PARASITES, IMMUNOGLOBULINS, MICROBIAL virulence, ANIMAL experimentation, CYTOKINES, ENZYME-linked immunosorbent assay, GENES, HEART, INTERFERONS, MICE, PROTOZOA, RESEARCH funding, TRYPANOSOMIASIS, VACCINES, PHENOMENOLOGICAL biology
Abstract

Background: Previous studies showed that a naturally attenuated strain from Trypanosoma cruzi triggers an immune response mainly related to a Th2-type profile. Albeit this, a strong protection against virulent challenge was obtained after priming mice with this attenuated strain. However, this protection is not enough to completely clear parasites from the host. In T. cruzi infection, early Interferon-gamma (IFN-γ) is critical to lead type 1 responses able to control intracellular parasites. Therefore we evaluated whether the co-administration of a plasmid encoding murine IFN-γ could modify the immune response induced by infection with attenuated parasites and improve protection against further infections.Methods: C57BL/6J mice were infected intraperitoneally with three doses of live attenuated parasites in combination with plasmid pVXVR-mIFN-γ. Before each infection dose, sera samples were collected for parasite specific antibodies determination and cytokine quantification. To evaluate the recall response to T. cruzi, mice were challenged with virulent parasites 30 days after the last dose and parasite load in peripheral blood and heart was evaluated.Results: As determined by ELISA, significantly increase in T. cruzi specific antibodies response was detected in the group in which pVXVR-mIFN-γ was incorporated, with a higher predominance of IgG2a subtype in comparison to the group of mice only inoculated with attenuated parasites. At our limit of detection, serum levels of IFN-γ were not detected, however a slight decrease in IL-10 concentrations was observed in groups in which pVXVR-mIFN-γ was supplemented. To analyze if the administration of pVXVR-mIFN-γ has any beneficial effect in protection against subsequent infections, all experimental groups were submitted to a lethal challenge with virulent bloodstream trypomastigotes. Similar levels of challenge parasites were detected in peripheral blood and heart of mice primed with attenuated parasites alone or combined with plasmid DNA. Expansion of IgG antibodies was not significant in TCC+ pVXVR-mIFN-γ; however, the overall tendency to sustain a Th2 profile was maintained.Conclusions: Overall, these results suggest that administration of plasmid pVXVR-mIFN-γ could have beneficial effects on host specific antibody production in response to T. cruzi attenuated infection; however, this outcome is not reflected in an improved protection against further virulent infections. [ABSTRACT FROM AUTHOR]

Published
2017
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26. Immunoenzymatic evaluation of the recombinant SAPA protein of Trypanosoma cruzi in naturally infected dogs

Author

Cimino, Rubén Oscar, Diosque, Patricio, López Quiroga, Inés R., Gil, José Fernando, and Nasser, Julio Rubén

Subjects
purl.org/becyt/ford/1 [https], Ciencias Biológicas, Otras Ciencias Biológicas, ANTÍGENO SAPA, ELISA, TRYPANOSOMA CRUZI, purl.org/becyt/ford/1.6 [https], CIENCIAS NATURALES Y EXACTAS, PERROS
Abstract

En este trabajo evaluamos el antígeno recombinante SAPA (Shed Acute Phase Antigen) para la detección de anticuerpos anti-Trypanosoma cruzi en sueros de perros infectados naturalmente. La técnica utilizada fue el ELISA y los antígenos utilizados fueron un homogenato de parásitos de T. cruzi (ELISA-H) y el recombinante SAPA (ELISA-SAPA). Se analizaron 93 sueros de perros por ELISA-H y ELISA-SAPA, los que fueron agrupados de la siguiente manera: grupo 1 (G1), 11 sueros controles negativos de la ciudad de Salta; grupo 2 (G2), 11 sueros controles positivos, pertenecientes a perros infectados naturalmente con T. cruzi; y grupo 3 (G3), 71 sueros de perros pertenecientes a zona endémica de infección chagásica. La sensibilidad y la especificidad del ELISA-SAPA fueron del 100 %. El índice kappa entre ELISA-H y ELISA-SAPA fue de 0,85. Estos resultados confirman que es adecuado el uso del antígeno recombinante SAPA para el diagnóstico de la infección por T. cruzi en perros. We evaluated the recombinant antigen SAPA (Shed Acute Phase Antigen) for the detection of Trypanosoma cruzi antibodies in sera from naturally infected dogs. The technique used was ELISA and the antigens were a homogenate of parasite T. cruzi (ELISA-H) and the recombinant SAPA (ELISA-SAPA). We analyzed 93 sera from dogs by ELISA-H and ELISA-SAPA, which were grouped as follows: G1: 11 negative control sera from the city of Salta, G2: 11 positive control sera from dogs naturally infected with T. cruzi and G3: 71 samples of dogs belonging to a Chagas disease-endemic area. The sensitivity and specificity of ELISA-SAPA were 100 %. The kappa index between ELISA-H and ELISA-SAPA was 0,85. These results confirm the use of SAPA antigen in the diagnosis of infection with T. cruzi in dogs. Fil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Escuela de Biología. Cátedra de Química Biológica; Argentina Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina Fil: López Quiroga, Inés R.. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Escuela de Biología. Cátedra de Química Biológica; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina Fil: Gil, José Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Nasser, Julio Rubén. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Escuela de Biología. Cátedra de Química Biológica; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina

Published
2012

27. Trypanosoma cruzi I haplogroups in american countries based on spliced leader-intergenic spacer polimorphism

Author

Cura, Carolina Inés, Mejía Jaramillo, Ana M., Duffy, Tomás, Burgos, Juan Miguel, Rodriguero, Marcela Silvina, Cardinal, Marta Victoria, Kjos, Sonia, Gurgel Gonçalves, Rodrigo, Blanchet, Denis, De Pablos, Luis M., Tomasini, Nicolás, da Silva, Alexandre, Russomando, Graciela Mabel, Cuba, Cesar A. Cuba, Aznar, Christine, Abate, Teresa, Levin, Mariano Jorge, Osuna, Antonio, Gurtler, Ricardo Esteban, Diosque, Patricio, Solari, Aldo, Triana Chávez, Omar, and Schijman, Alejandro Gabriel

Subjects
Ciencias Biológicas, purl.org/becyt/ford/1 [https], PCR, DISCRETE TYPING UNIT, Otras Ciencias Biológicas, CHAGAS DISEASE, SPLICED-LEADER INTERGENIC REGION, purl.org/becyt/ford/1.6 [https], CIENCIAS NATURALES Y EXACTAS, GENOTYPE, TRYPANOSOMA CRUZI I
Abstract

The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, allowing identification of 76 genotypes with 54 polymorphic sites from 123 aligned sequences. On the basis of the microsatellite motif proposed by Herrera et al. (2007) to define four haplotypes in Colombia, we could classify these genotypes into four distinct Tc I SL-IR groups, three corresponding to the former haplotypes Ia (11 genotypes), Ib (11 genotypes) and Id (35 genotypes); and one novel group, Ie (19 genotypes). Genotypes harbouring the Tc Ic motif were not detected in our study. Tc Ia was associated with domestic cycles in southern and northern South America and sylvatic cycles in Central and North America. Tc Ib was found in all transmission cycles from Colombia. Tc Id was identified in all transmission cycles from Argentina and Colombia, including Chagas cardiomyopathy patients, sylvatic Brazilian samples and human cases from French Guiana, Panama and Venezuela. Tc Ie gathered five samples from domestic Triatoma infestans from northern Argentina, nine samples from wild Mepraia spinolai and Mepraia gajardoi and two chagasic patients from Chile and one from a Bolivian patient with chagasic reactivation. Mixed infections by Tc Ia. +. Tc Id, Tc Ia. +. Tc Ie and Tc Id. +. Tc Ie were detected in vector faeces and isolates from human and vector samples. In addition, Tc Ia and Tc Id were identified in different tissues from a heart transplanted Chagas cardiomyopathy patient with reactivation, denoting histotropism. Trypanosoma cruzi I SL-IR genotypes from parasites infecting Triatoma gerstaeckeri and Didelphis virginiana from USA, T. infestans from Paraguay, Rhodnius nasutus and Rhodnius neglectus from Brazil and M. spinolai and M. gajardoi from Chile are to our knowledge described for the first time. Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Mejía Jaramillo, Ana M.. Universidad de Antioquia; Colombia Fil: Duffy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Burgos, Juan Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cardinal, Marta Victoria. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Kjos, Sonia. Centers for Disease Control and Prevention; Estados Unidos Fil: Gurgel Gonçalves, Rodrigo. Universidade do Brasília; Brasil Fil: Blanchet, Denis. Universite Des Antilles Et de la Guyane; Guayana Francesa Fil: De Pablos, Luis M.. Universidad de Granada; España Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: da Silva, Alexandre. Centers for Disease Control and Prevention; Estados Unidos Fil: Russomando, Graciela Mabel. Universidad Nacional de Asunción. Rectorado. Instituto de Investigaciones En Cs. de la Salud; Paraguay Fil: Cuba, Cesar A. Cuba. Universidade do Brasília; Brasil Fil: Aznar, Christine. Universite Des Antilles Et de la Guyane; Guayana Francesa Fil: Abate, Teresa. Universidad Central de Venezuela. Facultad de Medicina; Venezuela Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Osuna, Antonio. Universidad de Granada; España Fil: Gurtler, Ricardo Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Solari, Aldo. Facultad de Medicina de la Universidad de Chile; Chile Fil: Triana Chávez, Omar. Universidad de Antioquia; Colombia Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina

Published
2010

28. Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

Author

Ramírez, Juan Carlos, primary, Cura, Carolina Inés, additional, da Cruz Moreira, Otacilio, additional, Lages-Silva, Eliane, additional, Juiz, Natalia, additional, Velázquez, Elsa, additional, Ramírez, Juan David, additional, Alberti, Anahí, additional, Pavia, Paula, additional, Flores-Chávez, María Delmans, additional, Muñoz-Calderón, Arturo, additional, Pérez-Morales, Deyanira, additional, Santalla, José, additional, Marcos da Matta Guedes, Paulo, additional, Peneau, Julie, additional, Marcet, Paula, additional, Padilla, Carlos, additional, Cruz-Robles, David, additional, Valencia, Edward, additional, Crisante, Gladys Elena, additional, Greif, Gonzalo, additional, Zulantay, Inés, additional, Costales, Jaime Alfredo, additional, Alvarez-Martínez, Miriam, additional, Martínez, Norma Edith, additional, Villarroel, Rodrigo, additional, Villarroel, Sandro, additional, Sánchez, Zunilda, additional, Bisio, Margarita, additional, Parrado, Rudy, additional, Maria da Cunha Galvão, Lúcia, additional, Jácome da Câmara, Antonia Cláudia, additional, Espinoza, Bertha, additional, Alarcón de Noya, Belkisyole, additional, Puerta, Concepción, additional, Riarte, Adelina, additional, Diosque, Patricio, additional, Sosa-Estani, Sergio, additional, Guhl, Felipe, additional, Ribeiro, Isabela, additional, Aznar, Christine, additional, Britto, Constança, additional, Yadón, Zaida Estela, additional, and Schijman, Alejandro G., additional

Published
2015
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30. Experimental Evidence of Biological Interactions among Different Isolates of Trypanosoma cruzi from the Chaco Region

Author

Ragone, Paula G., primary, Pérez Brandán, Cecilia, additional, Monje Rumi, Mercedes, additional, Tomasini, Nicolás, additional, Lauthier, Juan J., additional, Cimino, Rubén O., additional, Uncos, Alejandro, additional, Ramos, Federico, additional, Alberti D´Amato, Anahí M., additional, Basombrío, Miguel A., additional, and Diosque, Patricio, additional

Published
2015
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31. Seroprevalence of Triatoma virus (Dicistroviridae: Cripaviridae) antibodies in Chagas disease patients

Author

Querido, Jailson FB, primary, Echeverría, María G, additional, Marti, Gerardo A, additional, Costa, Rita Medina, additional, Susevich, María L, additional, Rabinovich, Jorge E, additional, Copa, Aydee, additional, Montaño, Nair A, additional, Garcia, Lineth, additional, Cordova, Marisol, additional, Torrico, Faustino, additional, Sánchez-Eugenia, Rubén, additional, Sánchez-Magraner, Lissete, additional, Muñiz-Trabudua, Xabier, additional, López-Marijuan, Ibai, additional, Rozas-Dennis, Gabriela S, additional, Diosque, Patricio, additional, de Castro, Ana M, additional, Robello, Carlos, additional, Rodríguez, Julio S, additional, Altcheh, Jaime, additional, Salazar-Schettino, Paz M, additional, Bucio, Marta I, additional, Espinoza, Bertha, additional, Guérin, Diego MA, additional, and Silva, Marcelo Sousa, additional

Published
2015
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32. Trypanosoma cruzi diversity in the Gran Chaco: Mixed infections and differential host distribution of TcV and TcVI

Author

Monje-Rumi, María M., primary, Brandán, Cecilia Pérez, additional, Ragone, Paula G., additional, Tomasini, Nicolás, additional, Lauthier, Juan J., additional, Alberti D’Amato, Anahí M., additional, Cimino, Rubén O., additional, Orellana, Viviana, additional, Basombrío, Miguel A., additional, and Diosque, Patricio, additional

Published
2015
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33. Spliced leader RNA gene promoter sequence heterogeneity in CL-Brener Trypanosoma cruzi reference strain

Author

Cribb, Pamela, Tapia Paredes, Elizabeth, Diosque, Patricio, and Serra, Esteban Carlos

Subjects
Ciencias Biológicas, HYBRID STRAINS, Biología Celular, Microbiología, GENE PROMOTER, TRYPANOSOMA CRUZI, TAXONOMY, CIENCIAS NATURALES Y EXACTAS, SL RNA
Abstract

Trypanosoma cruzi is divided into two phylogenetic lineages, T. cruzi I and T. cruzi II, which contain different spliced leader (SL) RNA gene promoter sequences: Class I SL gene promoter sequences are found in T. cruzi II, and Class II sequences in T. cruzi I. We analysed different SL RNA promoter sequences from CL-Brener reference strain, belonging to T. cruzi II lineage, and detected sequences that differed within the -80/+1 highly conserved region. Indeed, many of these divergent SL promoters present features of T. cruzi I promoters. Some of these sequences were grouped into the T. cruzi I sequences clade by Bayesian analysis. The results presented herein show that sequence heterogeneity in SL RNA gene promoter not only exists between T. cruzi strains but also within CL-Brener strain. These CL-Brener "T. cruzi I-like" sequences could be considered a molecular trace of a hybrid origin of the SL RNA gene and a new evidence for the presence of sequences of T. cruzi I origin into a T. cruzi II strain. The possible origins of these sequences are discussed. Fil: Cribb, Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Tapia Paredes, Elizabeth. Universidad Nacional de Rosario; Argentina Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Serra, Esteban Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina

Published
2004

35. Optimized Multilocus Sequence Typing (MLST) Scheme for Trypanosoma cruzi

Author

Diosque, Patricio, primary, Tomasini, Nicolás, additional, Lauthier, Juan José, additional, Messenger, Louisa Alexandra, additional, Monje Rumi, María Mercedes, additional, Ragone, Paula Gabriela, additional, Alberti-D'Amato, Anahí Maitén, additional, Pérez Brandán, Cecilia, additional, Barnabé, Christian, additional, Tibayrenc, Michel, additional, Lewis, Michael David, additional, Llewellyn, Martin Stephen, additional, Miles, Michael Alexander, additional, and Yeo, Matthew, additional

Published
2014
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36. Development of Peptide-Based Lineage-Specific Serology for Chronic Chagas Disease: Geographical and Clinical Distribution of Epitope Recognition

Author

Bhattacharyya, Tapan, primary, Falconar, Andrew K., additional, Luquetti, Alejandro O., additional, Costales, Jaime A., additional, Grijalva, Mario J., additional, Lewis, Michael D., additional, Messenger, Louisa A., additional, Tran, Trang T., additional, Ramirez, Juan-David, additional, Guhl, Felipe, additional, Carrasco, Hernan J., additional, Diosque, Patricio, additional, Garcia, Lineth, additional, Litvinov, Sergey V., additional, and Miles, Michael A., additional

Published
2014
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37. The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study

Author

De Winne, Koen, primary, Büscher, Philippe, additional, Luquetti, Alejandro O., additional, Tavares, Suelene B. N., additional, Oliveira, Rodrigo A., additional, Solari, Aldo, additional, Zulantay, Ines, additional, Apt, Werner, additional, Diosque, Patricio, additional, Monje Rumi, Mercedes, additional, Gironès, Nuria, additional, Fresno, Manuel, additional, Lopez-Velez, Rogelio, additional, Perez-Molina, José A., additional, Monge-Maillo, Begoña, additional, Garcia, Lineth, additional, and Deborggraeve, Stijn, additional

Published
2014
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39. Congenital Chagas disease involves Trypanosoma cruzi sub-lineage IId in the northwestern province of Salta, Argentina

Author

Corrales, Rosa R.M., Mora, María Celia, Negrette, Olga Sanchez, Lacunza, Diego, Basombrio, Miguel Ángel M.A., Diosque, Patricio, Virreira Bermudez, Myrna, Brenière, Simone Frédérique Rédérique S.F., Corrales, Rosa R.M., Mora, María Celia, Negrette, Olga Sanchez, Lacunza, Diego, Basombrio, Miguel Ángel M.A., Diosque, Patricio, Virreira Bermudez, Myrna, and Brenière, Simone Frédérique Rédérique S.F.

Abstract

Trypanosoma cruzi is genetically classified into six discrete phylogenetic lineages on the basis of different genetic markers. Identifying lineages circulating among humans in different areas is essential to understand the molecular epidemiology of Chagas disease. In the present study, 18 T. cruzi isolates from congenitally infected newborns in the northwestern province of Salta-Argentina were studied by multilocus enzyme electrophoresis (MLEE) and random amplified polymorphic DNA (RAPD). All isolates were typed by MLEE and RAPD as belonging to T. cruzi IId. Analysis of minor variants of TcIId using probes hybridizing with hypervariable domains of kDNA minicircles, detected three variants with a similar distribution among the isolates. Our findings confirm the presence of T. cruzi IId among congenitally infected newborns in northwestern Argentina and support the assumption that human infection by T. cruzi in the Southern Cone countries of Latin America is due principally to T. cruzi II. © 2008 Elsevier B.V. All rights reserved., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2009

41. Epidemiology of American Tegumentary Leishmaniasis and Trypanosoma cruzi Infection in the Northwestern Argentina.

Author

Hoyos, Carlos L., Cajal, Silvana P., Juarez, Marisa, Marco, Jorge D., Alberti D’Amato, Anahí M., Cayo, Melina, Torrejón, Irma, Cimino, Rubén O., Diosque, Patricio, Krolewiecki, Alejandro J., Nasser, Julio R., and Gil, José F.

Subjects
AGE distribution, CONFIDENCE intervals, PROBABILITY theory, LEISHMANIASIS, PUBLIC health surveillance, RESEARCH, RESEARCH funding, STATISTICAL sampling, SEX distribution, OCCUPATIONAL hazards, TRYPANOSOMIASIS, ENVIRONMENTAL exposure, MULTIPLE regression analysis, DISEASE prevalence, CROSS-sectional method, CASE-control method, SEROPREVALENCE, DATA analysis software, DESCRIPTIVE statistics, ODDS ratio, MIXED infections, INFECTIOUS disease transmission, DISEASE risk factors
Abstract

Background. Endemic areas of tegumentary leishmaniasis (TL) in Salta, Argentina, present some overlap zones with the geographical distribution of Chagas disease, with mixed infection cases being often detected. Objectives. The purpose of this study was to determine the magnitude of Leishmania sp. infection and potential associated risk factors, the serologic prevalence of T. cruzi, and the presence of T. cruzi-Leishmania sp. mixed infection in a region of the northwest of Argentina. Methods. Cross-sectional studies were conducted to detect TL prevalence and T. cruzi seroprevalence. A case-control study was conducted to examine leishmaniasis risk factors. Results. Prevalence of TL was 0.17%, seroprevalence of T. cruzi infection was 9.73%, and mixed infection proportion—within the leishmaniasic patients group—was 16.67%. The risk factors associated with TL transmission were sex, age, exposure to bites at work, staying outdoors more than 10 hours/day, bathing in the river, and living with people who had lesions or were infected during the study. Discussion. The endemic pattern of TL seems to involve exposure of patients to vectors in wild as well as peridomestic environment. Cases of T. cruzi infection are apparently due to migration. Therefore, a careful epidemiological surveillance is necessary due to the contraindication of antimonial administration to chagasic patients. [ABSTRACT FROM AUTHOR]

Published
2016
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42. Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina

Author

Ragone, Paula G., primary, Pérez Brandán, Cecilia, additional, Padilla, Angel M., additional, Monje Rumi, Mercedes, additional, Lauthier, Juan J., additional, Alberti D’Amato, Anahí M., additional, Tomasini, Nicolás, additional, Cimino, Ruben O., additional, Romero, Nélida M., additional, Portelli, Marcela, additional, Nasser, Julio R., additional, Basombrío, Miguel A., additional, and Diosque, Patricio, additional

Published
2012
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43. Candidate targets for Multilocus Sequence Typing of Trypanosoma cruzi: Validation using parasite stocks from the Chaco Region and a set of reference strains

Author

Lauthier, Juan J., primary, Tomasini, Nicolás, additional, Barnabé, Christian, additional, Rumi, María M. Monje, additional, D’Amato, Anahí M. Alberti, additional, Ragone, Paula G., additional, Yeo, Matthew, additional, Lewis, Michael D., additional, Llewellyn, Martin S., additional, Basombrío, Miguel A., additional, Miles, Michael A., additional, Tibayrenc, Michel, additional, and Diosque, Patricio, additional

Published
2012
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45. Interest and limitations of Spliced Leader Intergenic Region sequences for analyzing Trypanosoma cruzi I phylogenetic diversity in the Argentinean Chaco

Author

Tomasini, Nicolás, primary, Lauthier, Juan J., additional, Rumi, María M. Monje, additional, Ragone, Paula G., additional, D’Amato, Anahí A. Alberti, additional, Brandan, Cecilia Pérez, additional, Cura, Carolina I., additional, Schijman, Alejandro G., additional, Barnabé, Christian, additional, Tibayrenc, Michel, additional, Basombrío, Miguel A., additional, Falla, Alejandra, additional, Herrera, Claudia, additional, Guhl, Felipe, additional, and Diosque, Patricio, additional

Published
2011
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46. International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

Author

Schijman, Alejandro G., primary, Bisio, Margarita, additional, Orellana, Liliana, additional, Sued, Mariela, additional, Duffy, Tomás, additional, Mejia Jaramillo, Ana M., additional, Cura, Carolina, additional, Auter, Frederic, additional, Veron, Vincent, additional, Qvarnstrom, Yvonne, additional, Deborggraeve, Stijn, additional, Hijar, Gisely, additional, Zulantay, Inés, additional, Lucero, Raúl Horacio, additional, Velazquez, Elsa, additional, Tellez, Tatiana, additional, Sanchez Leon, Zunilda, additional, Galvão, Lucia, additional, Nolder, Debbie, additional, Monje Rumi, María, additional, Levi, José E., additional, Ramirez, Juan D., additional, Zorrilla, Pilar, additional, Flores, María, additional, Jercic, Maria I., additional, Crisante, Gladys, additional, Añez, Néstor, additional, De Castro, Ana M., additional, Gonzalez, Clara I., additional, Acosta Viana, Karla, additional, Yachelini, Pedro, additional, Torrico, Faustino, additional, Robello, Carlos, additional, Diosque, Patricio, additional, Triana Chavez, Omar, additional, Aznar, Christine, additional, Russomando, Graciela, additional, Büscher, Philippe, additional, Assal, Azzedine, additional, Guhl, Felipe, additional, Sosa Estani, Sergio, additional, DaSilva, Alexandre, additional, Britto, Constança, additional, Luquetti, Alejandro, additional, and Ladzins, Janis, additional

Published
2011
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48. Genome-Scale Multilocus Microsatellite Typing of Trypanosoma cruzi Discrete Typing Unit I Reveals Phylogeographic Structure and Specific Genotypes Linked to Human Infection

Author

Llewellyn, Martin S., primary, Miles, Michael A., additional, Carrasco, Hernan J., additional, Lewis, Michael D., additional, Yeo, Matthew, additional, Vargas, Jorge, additional, Torrico, Faustino, additional, Diosque, Patricio, additional, Valente, Vera, additional, Valente, Sebastiao A., additional, and Gaunt, Michael W., additional

Published
2009
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