26 results on '"Dionora, Jacqueline"'
Search Results
2. Natural Diversity in Stomatal Features of Cultivated and Wild Oryza Species
- Author
-
Chatterjee, Jolly, Thakur, Vivek, Nepomuceno, Robert, Coe, Robert A., Dionora, Jacqueline, Elmido-Mabilangan, Abigail, Llave, Abraham Darius, Reyes, Anna Mae Delos, Monroy, Apollo Neil, Canicosa, Irma, Bandyopadhyay, Anindya, Jena, Kshirod K., Brar, Darshan S., and Quick, William Paul
- Published
- 2020
- Full Text
- View/download PDF
3. CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice
- Author
-
Yin, Xiaojia, Biswal, Akshaya K., Dionora, Jacqueline, Perdigon, Kristel M., Balahadia, Christian P., Mazumdar, Shamik, Chater, Caspar, Lin, Hsiang-Chun, Coe, Robert A., Kretzschmar, Tobias, Gray, Julie E., Quick, Paul W., and Bandyopadhyay, Anindya
- Published
- 2017
- Full Text
- View/download PDF
4. Phyllochron duration and changes through rice development shape the vertical leaf size profile
- Author
-
Pablo, Janet P., primary, Clerget, Benoit, additional, Bueno, Crisanta, additional, Dionora, Jacqueline, additional, Domingo, Abigail J., additional, De Guzman, Constancio C., additional, Aguilar, Edna A., additional, Cadiz, Nina M., additional, and Sta Cuz, Pompe C., additional
- Published
- 2022
- Full Text
- View/download PDF
5. Two forward genetic screens for vein density mutants in sorghum converge on a cytochrome P450 gene in the brassinosteroid pathway
- Author
-
Rizal, Govinda, Thakur, Vivek, Dionora, Jacqueline, Karki, Shanta, Wanchana, Samart, Acebron, Kelvin, Larazo, Nikki, Garcia, Richard, Mabilangan, Abigail, Montecillo, Florencia, Danila, Florence, Mogul, Reychelle, Pablico, Paquito, Leung, Hei, Langdale, Jane A., Sheehy, John, Kelly, Steven, and Quick, William Paul
- Published
- 2015
- Full Text
- View/download PDF
6. Dynamics of photosynthetic induction and relaxation within the canopy of rice and two wild relatives
- Author
-
Acevedo‐Siaca, Liana G., primary, Dionora, Jacqueline, additional, Laza, Rebecca, additional, Paul Quick, William, additional, and Long, Stephen P., additional
- Published
- 2021
- Full Text
- View/download PDF
7. A low CO2-responsive mutant of Setaria viridis reveals that reduced carbonic anhydrase limits C4 photosynthesis
- Author
-
Chatterjee, Jolly, primary, Coe, Robert A, additional, Acebron, Kelvin, additional, Thakur, Vivek, additional, Yennamalli, Ragothaman M, additional, Danila, Florence, additional, Lin, Hsiang-Chun, additional, Balahadia, Christian Paolo, additional, Bagunu, Efren, additional, Padhma, Preiya P O S, additional, Bala, Soumi, additional, Yin, Xiaojia, additional, Rizal, Govinda, additional, Dionora, Jacqueline, additional, Furbank, Robert T, additional, von Caemmerer, Susanne, additional, and Quick, William Paul, additional
- Published
- 2021
- Full Text
- View/download PDF
8. Additional file 2 of Natural Diversity in Stomatal Features of Cultivated and Wild Oryza Species
- Author
-
Chatterjee, Jolly, Thakur, Vivek, Nepomuceno, Robert, Coe, Robert A., Dionora, Jacqueline, Elmido-Mabilangan, Abigail, Llave, Abraham Darius, Reyes, Anna Mae Delos, Monroy, Apollo Neil, Canicosa, Irma, Anindya Bandyopadhyay, Kshirod K. Jena, Brar, Darshan S., and Quick, William Paul
- Subjects
food and beverages ,human activities - Abstract
Additional file 2: Table S1. Stomatal number, length, width and area in Oryza family. Table S2. Stomatal structural diversity in different Oryza complexes. Table S3. Diversity in guard cell length (GCL) and guard cell width (GCW) in rice family. Table S4. Guard cell diversity (%) in different Oryza complexes. Table S5. Abaxial and adaxial stomatal conductance (gmax), and carbon isotope discrimination (Δ13C) values in rice family. Table S6. Diversity in stomatal function in different Oryza complexes. Table S7. Accessory traits: Inter-stomatal distance, epidermal cell length (EPL) and width (EPW). Table S8. Leaf morphological traits, vein characters and total stomata. Table S9. Phylogenetic signal in stomatal traits.
- Published
- 2020
- Full Text
- View/download PDF
9. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions
- Author
-
Caine, Robert, Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya, Dionora, Jacqueline, Chater, Caspar, Coe, Robert, Bandyopadhyay, Anindya, Murchie, Erik, swarup, Ranjan, Quick, W. Paul, Gray, Julie, Caine, Robert, Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya, Dionora, Jacqueline, Chater, Caspar, Coe, Robert, Bandyopadhyay, Anindya, Murchie, Erik, swarup, Ranjan, Quick, W. Paul, and Gray, Julie
- Abstract
Much of humanity relies on rice (Oryza sativa) as a food source, but cultivation is water intensive and the crop is vulnerable to drought and high temperatures. Under climate change, periods of reduced water availability and high temperature are expected to become more frequent, leading to detrimental effects on rice yields
- Published
- 2019
10. Key changes in gene expression identified for different stages of C4 evolution in Alloteropsis semialata
- Author
-
Dunning, Luke T., Moreno-Villena, Jose J., Lundgren, Marjorie Ruth, Dionora, Jacqueline, Salazar, Paolo, Adams, Claire, Nyirenda, Florence, Olofsson, Jill K., Mapaura, Anthony, Grundy, Isla, Kayombo, Canisius, Dunning, Lucy, Kentatchime, Fabrice, Ariyarathne, Menaka, Yakandawala, Deepthi, Besnard, Guillaume, Quick, W. Paul, Brautigam, Andrea, Osborne, Colin P., Christin, Pascal-Antoine, Dunning, Luke T., Moreno-Villena, Jose J., Lundgren, Marjorie Ruth, Dionora, Jacqueline, Salazar, Paolo, Adams, Claire, Nyirenda, Florence, Olofsson, Jill K., Mapaura, Anthony, Grundy, Isla, Kayombo, Canisius, Dunning, Lucy, Kentatchime, Fabrice, Ariyarathne, Menaka, Yakandawala, Deepthi, Besnard, Guillaume, Quick, W. Paul, Brautigam, Andrea, Osborne, Colin P., and Christin, Pascal-Antoine
- Abstract
C4 photosynthesis is a complex trait that boosts productivity in tropical conditions. Compared to C3 species, the C4 state seems to require numerous novelties, but species comparisons can be confounded by long divergence times. Here, we exploit the photosynthetic diversity that exists within a single species, the grass Alloteropsis semialata, to detect changes in gene expression associated with different photosynthetic phenotypes. Phylogenetically-informed comparative transcriptomics show that intermediates with a weak C4 cycle are separated from the C3 phenotype by increases in the expression of 58 genes (0.22% of genes expressed in the leaves), including those encoding just three core C4 enzymes: ASP-AT, PCK, and PEPC. The subsequent transition to full C4 physiology was accompanied by increases in another 15 genes (0.06%), including only the core C4 enzyme PPDK. These changes likely created a rudimentary C4 physiology, and isolated populations subsequently improved this emerging C4 physiology, resulting in a patchwork of expression for some C4-accessory genes. Our work shows how C4 assembly in A. semialata happened in incremental steps, each requiring few alterations over the previous one. These create short bridges across adaptive landscapes that likely facilitated the recurrent origins of C4 photosynthesis through a gradual process of evolution.
- Published
- 2019
11. Lateral transfers of large DNA fragments spread functional genes among grasses
- Author
-
Dunning, Luke T, Olofsson, Jill K, Parisod, Christian, Choudhury, Rimjhim Roy, Moreno-Villena, Jose J, Yang, Yang, Dionora, Jacqueline, Quick, W Paul, Park, Minkyu, Bennetzen, Jeffrey L, Besnard, Guillaume, Nosil, Patrik, Osborne, Colin P, Christin, Pascal-Antoine, Dunning, Luke T, Olofsson, Jill K, Parisod, Christian, Choudhury, Rimjhim Roy, Moreno-Villena, Jose J, Yang, Yang, Dionora, Jacqueline, Quick, W Paul, Park, Minkyu, Bennetzen, Jeffrey L, Besnard, Guillaume, Nosil, Patrik, Osborne, Colin P, and Christin, Pascal-Antoine
- Abstract
A fundamental tenet of multicellular eukaryotic evolution is that vertical inheritance is paramount, with natural selection acting on genetic variants transferred from parents to offspring. This lineal process means that an organism's adaptive potential can be restricted by its evolutionary history, the amount of standing genetic variation, and its mutation rate. Lateral gene transfer (LGT) theoretically provides a mechanism to bypass many of these limitations, but the evolutionary importance and frequency of this process in multicellular eukaryotes, such as plants, remains debated. We address this issue by assembling a chromosome-level genome for the grass Alloteropsis semialata, a species surmised to exhibit two LGTs, and screen it for other grass-to-grass LGTs using genomic data from 146 other grass species. Through stringent phylogenomic analyses, we discovered 57 additional LGTs in the A. semialata nuclear genome, involving at least nine different donor species. The LGTs are clustered in 23 laterally acquired genomic fragments that are up to 170 kb long and have accumulated during the diversification of Alloteropsis. The majority of the 59 LGTs in A. semialata are expressed, and we show that they have added functions to the recipient genome. Functional LGTs were further detected in the genomes of five other grass species, demonstrating that this process is likely widespread in this globally important group of plants. LGT therefore appears to represent a potent evolutionary force capable of spreading functional genes among distantly related grass species.
- Published
- 2019
12. Key changes in gene expression identified for different stages of C4 evolution in Alloteropsis semialata
- Author
-
Dunning, Luke T, primary, Moreno-Villena, Jose J, additional, Lundgren, Marjorie R, additional, Dionora, Jacqueline, additional, Salazar, Paolo, additional, Adams, Claire, additional, Nyirenda, Florence, additional, Olofsson, Jill K, additional, Mapaura, Anthony, additional, Grundy, Isla M, additional, Kayombo, Canisius J, additional, Dunning, Lucy A, additional, Kentatchime, Fabrice, additional, Ariyarathne, Menaka, additional, Yakandawala, Deepthi, additional, Besnard, Guillaume, additional, Quick, W Paul, additional, Bräutigam, Andrea, additional, Osborne, Colin P, additional, and Christin, Pascal-Antoine, additional
- Published
- 2019
- Full Text
- View/download PDF
13. A low CO2-responsive mutant of Setaria viridis reveals that reduced carbonic anhydrase limits C4 photosynthesis.
- Author
-
Chatterjee, Jolly, Coe, Robert A, Acebron, Kelvin, Thakur, Vivek, Yennamalli, Ragothaman M, Danila, Florence, Lin, Hsiang-Chun, Balahadia, Christian Paolo, Bagunu, Efren, Padhma, Preiya P O S, Bala, Soumi, Yin, Xiaojia, Rizal, Govinda, Dionora, Jacqueline, Furbank, Robert T, Caemmerer, Susanne von, and Quick, William Paul
- Subjects
CARBONIC anhydrase ,PHOTOSYNTHESIS ,SETARIA ,PYRUVATE carboxylase ,CARBON isotopes ,MALATE dehydrogenase - Abstract
In C
4 species, β-carbonic anhydrase (CA), localized to the cytosol of the mesophyll cells, accelerates the interconversion of CO2 to HCO3 – , the substrate used by phospho enol pyruvate carboxylase (PEPC) in the first step of C4 photosynthesis. Here we describe the identification and characterization of low CO2 -responsive mutant 1 (lcr1) isolated from an N -nitroso- N -methylurea- (NMU) treated Setaria viridis mutant population. Forward genetic investigation revealed that the mutated gene Sevir.5G247800 of lcr1 possessed a single nucleotide transition from cytosine to thymine in a β-CA gene causing an amino acid change from leucine to phenylalanine. This resulted in severe reduction in growth and photosynthesis in the mutant. Both the CO2 compensation point and carbon isotope discrimination values of the mutant were significantly increased. Growth of the mutants was stunted when grown under ambient p CO2 but recovered at elevated p CO2 . Further bioinformatics analyses revealed that the mutation has led to functional changes in one of the conserved residues of the protein, situated near the catalytic site. CA transcript accumulation in the mutant was 80% lower, CA protein accumulation 30% lower, and CA activity ~98% lower compared with the wild type. Changes in the abundance of other primary C4 pathway enzymes were observed; accumulation of PEPC protein was significantly increased and accumulation of malate dehydrogenase and malic enzyme decreased. The reduction of CA protein activity and abundance in lcr1 restricts the supply of bicarbonate to PEPC, limiting C4 photosynthesis and growth. This study establishes Sevir.5G247800 as the major CA allele in Setaria for C4 photosynthesis and provides important insights into the function of CA in C4 photosynthesis that would be required to generate a rice plant with a functional C4 biochemical pathway. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
14. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions
- Author
-
Caine, Robert S., primary, Yin, Xiaojia, additional, Sloan, Jennifer, additional, Harrison, Emily L., additional, Mohammed, Umar, additional, Fulton, Timothy, additional, Biswal, Akshaya K., additional, Dionora, Jacqueline, additional, Chater, Caspar C., additional, Coe, Robert A., additional, Bandyopadhyay, Anindya, additional, Murchie, Erik H., additional, Swarup, Ranjan, additional, Quick, W. Paul, additional, and Gray, Julie E., additional
- Published
- 2018
- Full Text
- View/download PDF
15. High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points
- Author
-
Coe, Robert A., primary, Chatterjee, Jolly, additional, Acebron, Kelvin, additional, Dionora, Jacqueline, additional, Mogul, Reychelle, additional, Lin, HsiangChun, additional, Yin, Xiaojia, additional, Bandyopadhyay, Anindya, additional, Sirault, Xavier R. R., additional, Furbank, Robert T., additional, and Quick, W. Paul, additional
- Published
- 2018
- Full Text
- View/download PDF
16. Key changes in gene expression identified for different stages of C 4 evolution in Alloteropsis semialata.
- Author
-
Dunning, Luke T, Moreno-Villena, Jose J, Lundgren, Marjorie R, Dionora, Jacqueline, Salazar, Paolo, Adams, Claire, Nyirenda, Florence, Olofsson, Jill K, Mapaura, Anthony, Grundy, Isla M, Kayombo, Canisius J, Dunning, Lucy A, Kentatchime, Fabrice, Ariyarathne, Menaka, Yakandawala, Deepthi, Besnard, Guillaume, Quick, W Paul, Bräutigam, Andrea, Osborne, Colin P, and Christin, Pascal-Antoine
- Subjects
GENE expression ,TROPICAL conditions ,PHYSIOLOGY ,BIOLOGICAL evolution ,ASPARTATE aminotransferase ,PHENOTYPES - Abstract
C
4 photosynthesis is a complex trait that boosts productivity in tropical conditions. Compared with C3 species, the C4 state seems to require numerous novelties, but species comparisons can be confounded by long divergence times. Here, we exploit the photosynthetic diversity that exists within a single species, the grass Alloteropsis semialata , to detect changes in gene expression associated with different photosynthetic phenotypes. Phylogenetically informed comparative transcriptomics show that intermediates with a weak C4 cycle are separated from the C3 phenotype by increases in the expression of 58 genes (0.22% of genes expressed in the leaves), including those encoding just three core C4 enzymes: aspartate aminotransferase, phosphoenolpyruvate carboxykinase, and phosphoenolpyruvate carboxylase. The subsequent transition to full C4 physiology was accompanied by increases in another 15 genes (0.06%), including only the core C4 enzyme pyruvate orthophosphate dikinase. These changes probably created a rudimentary C4 physiology, and isolated populations subsequently improved this emerging C4 physiology, resulting in a patchwork of expression for some C4 accessory genes. Our work shows how C4 assembly in A. semialata happened in incremental steps, each requiring few alterations over the previous step. These create short bridges across adaptive landscapes that probably facilitated the recurrent origins of C4 photosynthesis through a gradual process of evolution. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
17. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions.
- Author
-
Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, and Gray, Julie E.
- Subjects
DROUGHT tolerance ,PHOTOSYNTHESIS ,PHYSIOLOGICAL effects of heat ,WATER conservation ,CLIMATE change - Abstract
Summary: Much of humanity relies on rice (Oryza sativa) as a food source, but cultivation is water intensive and the crop is vulnerable to drought and high temperatures. Under climate change, periods of reduced water availability and high temperature are expected to become more frequent, leading to detrimental effects on rice yields.We engineered the high‐yielding rice cultivar 'IR64' to produce fewer stomata by manipulating the level of a developmental signal. We overexpressed the rice epidermal patterning factor OsEPF1, creating plants with substantially reduced stomatal density and correspondingly low stomatal conductance.Low stomatal density rice lines were more able to conserve water, using c. 60% of the normal amount between weeks 4 and 5 post germination. When grown at elevated atmospheric CO2, rice plants with low stomatal density were able to maintain their stomatal conductance and survive drought and high temperature (40°C) for longer than control plants. Low stomatal density rice gave equivalent or even improved yields, despite a reduced rate of photosynthesis in some conditions.Rice plants with fewer stomata are drought tolerant and more conservative in their water use, and they should perform better in the future when climate change is expected to threaten food security. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
18. The Evolutionary Basis of Naturally Diverse Rice Leaves Anatomy
- Author
-
Chatterjee, Jolly, primary, Dionora, Jacqueline, additional, Elmido-Mabilangan, Abigail, additional, Wanchana, Samart, additional, Thakur, Vivek, additional, Bandyopadhyay, Anindya, additional, Brar, Darshan S., additional, and Quick, William Paul, additional
- Published
- 2016
- Full Text
- View/download PDF
19. High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO2 compensation points.
- Author
-
Coe, Robert A., Chatterjee, Jolly, Acebron, Kelvin, Dionora, Jacqueline, Mogul, Reychelle, Lin, HsiangChun, Yin, Xiaojia, Bandyopadhyay, Anindya, Sirault, Xavier R. R., Furbank, Robert T., and Quick, W. Paul
- Subjects
CHLOROPHYLL spectra ,PHOTOSYNTHESIS ,RICE ,PLANT mutation ,PHENOTYPES ,CARBON 4 photosynthesis - Abstract
To assist with efforts to engineer a C
4 photosynthetic pathway into rice, forward-genetic approaches are being used to identify the genes modulating key C4 traits. Currently, a major challenge is how to screen for a variety of different traits in a high-throughput manner. Here we describe a method for identifying C4 mutant plants with increased CO2 compensation points. This is used as a signature for decreased photosynthetic efficiency associated with a loss of C4 function. By exposing plants to a CO2 concentration close to the CO2 compensation point of a wild-type plant, individuals can be identified from measurements of chlorophyll a fluorescence. We use this method to screen a mutant population of the C4 monocot Setaria viridis (L.) P.Beauv. generated using N-nitroso-N-methylurea (NMU). Mutants were identified at a frequency of 1 per 157 lines screened. Forty-six candidate lines were identified and one line with a heritable homozygous phenotype selected for further characterisation. The CO2 compensation point of this mutant was increased to a value similar to that of C3 rice. Photosynthesis and growth was significantly reduced under ambient conditions. These data indicate that the screen was capable of identifying mutants with decreased photosynthetic efficiency. Characterisation and next-generation sequencing of all the mutants identified in this screen may lead to the discovery of novel genes underpinning C4 photosynthesis. These can be used to engineer a C4 photosynthetic pathway into rice. Engineering rice to perform a more efficient form of photosynthesis could increase yields by roughly 50%. Identifying the genes controlling this supercharged C4 photosynthetic process is a significant challenge. Using a rapid and efficient screening method we identify a large number of mutant plants that can be used to reverse engineer and understand this process. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
20. Lateral transfers of large DNA fragments spread functional genes among grasses
- Author
-
Dunning, Luke T., Olofsson, Jill K., Parisod, Christian Gérard, Choudhury, Rimjhim Roy, Moreno-Villena, Jose J., Yang, Yang, Dionora, Jacqueline, Quick, W. Paul, Park, Minkyu, Bennetzen, Jeffrey L., Besnard, Guillaume, Nosil, Patrik, Osborne, Colin P., and Christin, Pascal-Antoine
- Subjects
15. Life on land ,580 Plants (Botany) - Abstract
In multicellular organisms, exchange of genetic information occurs mainly among individuals belonging to the same species through sexual reproduction. Lateral gene transfer between distantly related taxa has been demonstrated in some cases, but its frequency and evolutionary importance have been controversial. By comparing genomes of many grasses, we show that large blocks of DNA containing functional genes are laterally passed among distantly related species. Some of these genes are then used by the recipient species, expanding their genetic toolkit. The spread of functional genes across grasses that have developed distinct physiological and ecological adaptations may therefore represent a significant evolutionary driving force in this globally important group of plants.
21. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions
- Author
-
Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, Gray, Julie E., Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, and Gray, Julie E.
- Abstract
Much of humanity relies on rice (Oryza sativa) as a food source, but cultivation is water intensive and the crop is vulnerable to drought and high temperatures. Under climate change, periods of reduced water availability and high temperature are expected to become more frequent, leading to detrimental effects on rice yields. We engineered the high-yielding rice cultivar ‘IR64’ to produce fewer stomata by manipulating the level of a developmental signal. We overexpressed the rice epidermal patterning factor OsEPF1, creating plants with substantially reduced stomatal density and correspondingly low stomatal conductance. Low stomatal density rice lines were more able to conserve water, using c. 60% of the normal amount between weeks 4 and 5 post germination. When grown at elevated atmospheric CO2, rice plants with low stomatal density were able to maintain their stomatal conductance and survive drought and high temperature (40°C) for longer than control plants. Low stomatal density rice gave equivalent or even improved yields, despite a reduced rate of photosynthesis in some conditions. Rice plants with fewer stomata are drought tolerant and more conservative in their water use, and they should perform better in the future when climate change is expected to threaten food security.
- Full Text
- View/download PDF
22. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions
- Author
-
Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, Gray, Julie E., Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, and Gray, Julie E.
- Abstract
Much of humanity relies on rice (Oryza sativa) as a food source, but cultivation is water intensive and the crop is vulnerable to drought and high temperatures. Under climate change, periods of reduced water availability and high temperature are expected to become more frequent, leading to detrimental effects on rice yields. We engineered the high-yielding rice cultivar ‘IR64’ to produce fewer stomata by manipulating the level of a developmental signal. We overexpressed the rice epidermal patterning factor OsEPF1, creating plants with substantially reduced stomatal density and correspondingly low stomatal conductance. Low stomatal density rice lines were more able to conserve water, using c. 60% of the normal amount between weeks 4 and 5 post germination. When grown at elevated atmospheric CO2, rice plants with low stomatal density were able to maintain their stomatal conductance and survive drought and high temperature (40°C) for longer than control plants. Low stomatal density rice gave equivalent or even improved yields, despite a reduced rate of photosynthesis in some conditions. Rice plants with fewer stomata are drought tolerant and more conservative in their water use, and they should perform better in the future when climate change is expected to threaten food security.
- Full Text
- View/download PDF
23. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions
- Author
-
Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, Gray, Julie E., Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, and Gray, Julie E.
- Abstract
Much of humanity relies on rice (Oryza sativa) as a food source, but cultivation is water intensive and the crop is vulnerable to drought and high temperatures. Under climate change, periods of reduced water availability and high temperature are expected to become more frequent, leading to detrimental effects on rice yields. We engineered the high-yielding rice cultivar ‘IR64’ to produce fewer stomata by manipulating the level of a developmental signal. We overexpressed the rice epidermal patterning factor OsEPF1, creating plants with substantially reduced stomatal density and correspondingly low stomatal conductance. Low stomatal density rice lines were more able to conserve water, using c. 60% of the normal amount between weeks 4 and 5 post germination. When grown at elevated atmospheric CO2, rice plants with low stomatal density were able to maintain their stomatal conductance and survive drought and high temperature (40°C) for longer than control plants. Low stomatal density rice gave equivalent or even improved yields, despite a reduced rate of photosynthesis in some conditions. Rice plants with fewer stomata are drought tolerant and more conservative in their water use, and they should perform better in the future when climate change is expected to threaten food security.
- Full Text
- View/download PDF
24. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions
- Author
-
Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, Gray, Julie E., Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, and Gray, Julie E.
- Abstract
Much of humanity relies on rice (Oryza sativa) as a food source, but cultivation is water intensive and the crop is vulnerable to drought and high temperatures. Under climate change, periods of reduced water availability and high temperature are expected to become more frequent, leading to detrimental effects on rice yields. We engineered the high-yielding rice cultivar ‘IR64’ to produce fewer stomata by manipulating the level of a developmental signal. We overexpressed the rice epidermal patterning factor OsEPF1, creating plants with substantially reduced stomatal density and correspondingly low stomatal conductance. Low stomatal density rice lines were more able to conserve water, using c. 60% of the normal amount between weeks 4 and 5 post germination. When grown at elevated atmospheric CO2, rice plants with low stomatal density were able to maintain their stomatal conductance and survive drought and high temperature (40°C) for longer than control plants. Low stomatal density rice gave equivalent or even improved yields, despite a reduced rate of photosynthesis in some conditions. Rice plants with fewer stomata are drought tolerant and more conservative in their water use, and they should perform better in the future when climate change is expected to threaten food security.
- Full Text
- View/download PDF
25. Rice with reduced stomatal density conserves water and has improved drought tolerance under future climate conditions
- Author
-
Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, Gray, Julie E., Caine, Robert S., Yin, Xiaojia, Sloan, Jennifer, Harrison, Emily L., Mohammed, Umar, Fulton, Timothy, Biswal, Akshaya K., Dionora, Jacqueline, Chater, Caspar C., Coe, Robert A., Bandyopadhyay, Anindya, Murchie, Erik H., Swarup, Ranjan, Quick, W. Paul, and Gray, Julie E.
- Abstract
Much of humanity relies on rice (Oryza sativa) as a food source, but cultivation is water intensive and the crop is vulnerable to drought and high temperatures. Under climate change, periods of reduced water availability and high temperature are expected to become more frequent, leading to detrimental effects on rice yields. We engineered the high-yielding rice cultivar ‘IR64’ to produce fewer stomata by manipulating the level of a developmental signal. We overexpressed the rice epidermal patterning factor OsEPF1, creating plants with substantially reduced stomatal density and correspondingly low stomatal conductance. Low stomatal density rice lines were more able to conserve water, using c. 60% of the normal amount between weeks 4 and 5 post germination. When grown at elevated atmospheric CO2, rice plants with low stomatal density were able to maintain their stomatal conductance and survive drought and high temperature (40°C) for longer than control plants. Low stomatal density rice gave equivalent or even improved yields, despite a reduced rate of photosynthesis in some conditions. Rice plants with fewer stomata are drought tolerant and more conservative in their water use, and they should perform better in the future when climate change is expected to threaten food security.
- Full Text
- View/download PDF
26. High-throughput chlorophyll fluorescence screening of Setaria viridis for mutants with altered CO 2 compensation points.
- Author
-
Coe RA, Chatterjee J, Acebron K, Dionora J, Mogul R, Lin H, Yin X, Bandyopadhyay A, Sirault XRR, Furbank RT, and Quick WP
- Abstract
To assist with efforts to engineer a C4 photosynthetic pathway into rice, forward-genetic approaches are being used to identify the genes modulating key C4 traits. Currently, a major challenge is how to screen for a variety of different traits in a high-throughput manner. Here we describe a method for identifying C4 mutant plants with increased CO2 compensation points. This is used as a signature for decreased photosynthetic efficiency associated with a loss of C4 function. By exposing plants to a CO2 concentration close to the CO2 compensation point of a wild-type plant, individuals can be identified from measurements of chlorophyll a fluorescence. We use this method to screen a mutant population of the C4 monocot Setaria viridis (L.)P.Beauv. generated using N-nitroso-N-methylurea (NMU). Mutants were identified at a frequency of 1 per 157 lines screened. Forty-six candidate lines were identified and one line with a heritable homozygous phenotype selected for further characterisation. The CO2 compensation point of this mutant was increased to a value similar to that of C3 rice. Photosynthesis and growth was significantly reduced under ambient conditions. These data indicate that the screen was capable of identifying mutants with decreased photosynthetic efficiency. Characterisation and next-generation sequencing of all the mutants identified in this screen may lead to the discovery of novel genes underpinning C4 photosynthesis. These can be used to engineer a C4 photosynthetic pathway into rice.
- Published
- 2018
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.