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380 results on '"Dinten, Jean-Marc"'

1. The SOLUS instrument: Optical characterization of the first hand-held probe for multimodal imaging (ultrasound and multi-wavelength time-resolved diffuse optical tomography)

Author

Maffeis, Giulia, Di Sieno, Laura, Dalla Mora, Alberto, Pifferi, Antonio, Tosi, Alberto, Conca, Enrico, Giudice, Andrea, Ruggeri, Alessandro, Tisa, Simone, Flocke, Alexander, Rosinski, Bogdan, Dinten, Jean-Marc, Perriollat, Mathieu, Lavaud, Jonathan, Arridge, Simon, Di Sciacca, Giuseppe, Farina, Andrea, Panizza, Pietro, Venturini, Elena, Gordebeke, Peter, and Taroni, Paola

Published
2024
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2. Optical elastic scattering for early label-free identification of clinical pathogens

Author

Genuer, Valentin, Gal, Olivier, Méteau, Jérémy, Marcoux, Pierre, Schultz, Emmanuelle, Lacot, Éric, Maurin, Max, and Dinten, Jean-Marc

Subjects
Physics - Biological Physics, Physics - Optics
Abstract

We report here on the ability of elastic light scattering in discriminating Gram+, Gram-and yeasts at an early stage of growth (6h). Our technique is non-invasive, low cost and does require neither skilled operators nor reagents. Therefore it is compatible with automation. It is based on the analysis of the scattering pattern (scatterogram) generated by a bacterial microcolony growing on agar, when placed in the path of a laser beam. Measurements are directly performed on closed Petri dishes. The characteristic features of a given scatterogram are first computed by projecting the pattern onto the Zernike orthogonal basis. Then the obtained data are compared to a database so that machine learning can yield identification result. A 10-fold cross-validation was performed on a database over 8 species (15 strains, 1906 scatterograms), at 6h of incubation. It yielded a 94% correct classification rate between Gram+, Gram-and yeasts. Results can be improved by using a more relevant function basis for projections, such as Fourier-Bessel functions. A fully integrated instrument has been installed at the Grenoble hospital's laboratory of bacteriology and a validation campaign has been started for the early screening of SA and MRSA (Staphylococcus aureus, methicillin-resistant S. aureus) carriers. Up to now, all the published studies about elastic scattering were performed in a forward mode, which is restricted to transparent media. However, in clinical diagnostics, most of media are opaque, such as blood-supplemented agar. That is why we propose a novel scheme capable of collecting back-scattered light which provides comparable results.

Published
2016
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3. Optical Detection and Sizing of Single Nanoparticles Using Continuous Wetting Films

Author

Hennequin, Yves, Allier, Cédric P, McLeod, Euan, Mudanyali, Onur, Migliozzi, Daniel, Ozcan, Aydogan, and Dinten, Jean-Marc

Subjects
Physical Sciences, Biomedical and Clinical Sciences, Engineering, Nanotechnology, Bioengineering, Equipment Design, Equipment Failure Analysis, Granulovirus, Image Enhancement, Lenses, Membranes, Artificial, Microscopy, Nanoparticles, Reproducibility of Results, Sensitivity and Specificity, Staphylococcus epidermidis, Wettability, wetting films, optical microscopy, computational imaging, nanoparticle detection, virus detection, bacteria Imaging, Nanoscience & Nanotechnology
Abstract

The physical interaction between nanoscale objects and liquid interfaces can create unique optical properties, enhancing the signatures of the objects with subwavelength features. Here we show that the evaporation on a wetting substrate of a polymer solution containing submicrometer or nanoscale particles creates liquid microlenses that arise from the local deformations of the continuous wetting film. These microlenses have properties similar to axicon lenses that are known to create beams with a long depth of focus. This enhanced depth of focus allows detection of single nanoparticles using a low-magnification microscope objective lens, achieving a relatively wide field-of-view, while also lifting the constraints on precise focusing onto the object plane. Hence, by creating these liquid axicon lenses through spatial deformations of a continuous thin wetting film, we transfer the challenge of imaging individual nanoparticles to detecting the light focused by these lenses. As a proof of concept, we demonstrate the detection and sizing of single nanoparticles (100 and 200 nm), CpGV granuloviruses, as well as Staphylococcus epidermidis bacteria over a wide field-of-view of 5.10 × 3.75 mm(2) using a 5× objective lens with a numerical aperture of 0.15. In addition to conventional lens-based microscopy, this continuous wetting-film-based approach is also applicable to lens-free computational on-chip imaging, which can be used to detect single nanoparticles over a large field-of-view of >20-30 mm(2). These results could be especially useful for high-throughput field analysis of nanoscale objects using compact and cost-effective microscope designs.

Published
2013

5. Initial examples of the SOLUS multimodal potential

Author

Maffeis, Giulia, primary, Pifferi, Antonio, additional, Dalla Mora, Alberto, additional, Di Sieno, Laura, additional, Cubeddu, Rinaldo, additional, Tosi, Alberto, additional, Conca, Enrico, additional, Giudice, Andrea, additional, Ruggeri, Alessandro, additional, Tisa, Simone, additional, Flocke, Alexander, additional, Rosinski, Bogdan, additional, Dinten, Jean-Marc, additional, Perriollat, Mathieu, additional, Fraschini, Christophe, additional, Lavaud, Jonathan, additional, Arridge, Simon, additional, Di Sciacca, Giuseppe, additional, Farina, Andrea, additional, Panizza, Pietro, additional, Venturini, Elena, additional, Gordebeke, Peter, additional, and Taroni, Paola, additional

Published
2023
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6. Breast lesion classification based on absorption and composition parameters: a look at SOLUS first outcomes

Author

Maffeis, Giulia, primary, Pifferi, Antonio, additional, Dalla Mora, Alberto D., additional, Di Sieno, Laura, additional, Cubeddu, Rinaldo, additional, Tosi, Alberto, additional, Conca, Enrico, additional, Giudice, Andrea, additional, Ruggeri, Alessandro, additional, Tisa, Simone, additional, Flocke, Alexander, additional, Rosinski, Bogdan, additional, Dinten, Jean-Marc, additional, Perriollat, Mathieu, additional, Fraschini, Christophe, additional, Lavaud, Jonathan, additional, Arridge, Simon, additional, Di Sciacca, Giuseppe, additional, Farina, Andrea, additional, Panizza, Pietro, additional, Venturini, Elena, additional, Gordebeke, Peter, additional, and Taroni, Paola, additional

Published
2023
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9. SOLUS: a novel multimodal approach to ultrasound and diffuse optics imaging of breast cancer

Author

Pifferi, Antonio, primary, Dalla Mora, Alberto, additional, Di Sieno, Laura, additional, Maffeis, Giulia, additional, Tosi, Alberto, additional, Conca, Enrico, additional, Sesta, Vincenzo, additional, Giudice, Andrea, additional, Ruggeri, Alessandro, additional, Tisa, Simone, additional, Flocke, Alexander, additional, Rosinski, Bogdan, additional, Dinten, Jean-Marc, additional, Perriollat, Mathieu, additional, Sportouche, Hélène, additional, Fraschini, Christophe, additional, Arridge, Simon, additional, Di Sciacca, Giuseppe, additional, Farina, Andrea, additional, Panizza, Pietro, additional, Venturini, Elena, additional, Gordebeke, Peter, additional, Zolda, Pamela, additional, and Taroni, Paola, additional

Published
2021
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11. Model reduction using wavelet multiresolution technique applied to fluorescence diffuse optical tomography

Author

Landragin-Frassati, Anne, Dinten, Jean-Marc, Georges, Didier, and Da Silva, Anabela

Subjects
Optics -- Research, Fluorescence -- Methods, Optical tomography -- Methods, Astronomy, Physics
Abstract

Fluorescence diffuse optical tomography is a powerful tool for the investigation of molecular events in studies for new therapeutic developments. Here, the stress is put on the mathematical problem of tomography, which can be formulated in terms of an estimation of physical parameters appearing as a set of partial differential equations and solved by the finite element method. This method is well known to be time consuming, and our principal objective is to reduce the model in order to speed up computation. A method based on a wavelet multiresolution technique is presented in detail. A validation study was conducted on synthetic data and experiments. [c] 2009 Optical Society of America OCIS codes: 000.4430, 100.7410, 170.3880.

Published
2009

12. Optical calibration protocol for an x-ray and optical multimodality tomography system dedicated to small-animal examination

Author

Da Silva, Anabela, Leabad, Mehdi, Driol, Clemence, Bordy, Thomas, Debourdeau, Mathieu, Dinten, Jean-Marc, Peltie, Philippe, and Rizo, Philippe

Subjects
Animal experimentation -- Methods, Calibration -- Methods, X-rays -- Usage, Optical tomography -- Usage, Optical tomography -- Methods, Astronomy, Physics
Abstract

A small-animal multimodality tomography system dedicated to the coregistration of fluorescence optical signal and x-ray measurements has been developed in our laboratory. The purpose of such a system is to offer the possibility of getting in vivo anatomical and functional information simultaneously. Moreover, anatomical measurements can be used as a regularization factor to achieve more accurate reconstructions of the biodistribution of fluorochromes and to speed up treatment. A dedicated acquisition protocol has been established, and the methodology of the reconstruction of the three-dimensional distribution of the biomarkers under cylindrical geometry consistent with classic computed tomography has been implemented. A phantom study was conducted to evaluate and to fix the parameters for the coregistration. These test experiments were reproduced by considering anesthetized mice that had thin glass tubes containing fluorochromes inserted into their esophagus. The instrument is also used for an in vivo biological study conducted on mice with lung tumors, tagged with near-infrared optical probes (targeting probes such as Transferin-AlexaFluor750). OCIS codes: 170.3880, 110.6955, 110.4190, 170.6280, 170.7440, 170.3660.

Published
2009

13. SOLUS: an innovative multimodal imaging system to improve breast cancer diagnosis through diffuse optics and ultrasounds

Author

Pifferi, Antonio, primary, Dalla Mora, Alberto, additional, Di Sieno, Laura, additional, Ferocino, Edoardo, additional, Tosi, Alberto, additional, Conca, Enrico, additional, Sesta, Vincenzo, additional, Giudice, Andrea, additional, Ruggeri, Alessandro, additional, Tisa, Simone, additional, Flocke, Alexander, additional, Rosinski, Bogdan, additional, Dinten, Jean-Marc, additional, Perriollat, Mathieu, additional, Fraschini, Christophe, additional, Sportouche, Hélène, additional, Arridge, Simon, additional, Di Sciacca, Giuseppe, additional, Farina, Andrea, additional, Panizza, Pietro, additional, Venturini, Elena, additional, Gordebeke, Peter, additional, Zolda, Pamela, additional, and Taroni, Paola, additional

Published
2021
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14. Estimation of optical properties of turbid media: experimental comparison of spatially and temporally resolved reflectance methods

Author

Falconet, Julie, Laidevant, Aurelie, Sablong, Raphael, da Silva, Anabela, Berger, Michel, Jaillon, Franck, Perrin, Emmanuel, Dinten, Jean-Marc, and Saint-Jalmes, Herve

Subjects
Reflectance -- Measurement, Absorption of light -- Research, Light scattering -- Research, Astronomy, Physics
Abstract

We compare two methods for the optical characterization of turbid media. The estimates of the absorption and reduced scattering coefficients ([[mu].sub.a] and [[mu]'.sub.s]) by a spatially resolved method and a time-resolved method are performed on tissue-like phantoms. Aqueous suspension of microspheres and Intralipid are used as scattering media with the addition of ink as an absorber. [[mu]'.sub.s] is first measured on weakly absorbing media. The robustness of these measurements is then tested with respect to a variation of [[mu].sub.a]. The spatially resolved method gave more accurate estimates for [[mu]'.sub.s] whereas the time-resolved method gave better results for [[mu].sub.a] estimates. OCIS codes: 170.5280, 170.3660, 170.7050, 170.6920, 170.3880.

Published
2008

17. Effects of the surface boundary on the determination of the optical properties of a turbid medium with time-resolved reflectance

Author

Laidevant, Aurelie, da Silva, Anabela, Berger, Michel, and Dinten, Jean-Marc

Subjects
Optics -- Research, Diffusion -- Research, Astronomy, Physics
Abstract

Solutions of the time-dependent diffusion equation were developed to take into account the depth of the source and the detector inside a semi-infinite medium. These solutions permitted an evaluation of optical properties at different depths below the surface by fitting time-resolved data. Measurements were performed on liquid optical phantoms with optical fibers for delivering and collecting light. A time-correlated single-photon-counting chain was used for electronic detection. The determination of optical properties underlines the continuity between the surface model and the infinite model and shows the depth at which the derived solutions can be applied. OCIS codes: 170.6920, 170.5280, 170.7050, 170.3660.

Published
2006

18. Contact, high-resolution spatial diffuse reflectance imaging system for skin condition diagnosis: a first-in-human clinical trial

Author

Koenig, Anne, primary, Petitdidier, Nils, additional, Grateau, Henri, additional, Characoun, Samarmar, additional, Ghaith, Abdallah, additional, Verges, Samuel, additional, Doutreleau, Stéphane, additional, Gharbi, Sadok, additional, Gerbelot, Rémi, additional, Gioux, Sylvain, additional, and Dinten, Jean-Marc, additional

Published
2021
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20. SOLUS Project: Bringing Innovation into Breast Cancer Diagnosis and in the Time-Domain Diffuse Optical Field

Author

Di Sieno, Laura, primary, Mora, Alberto Dalla, additional, Ferocino, Edoardo, additional, Pifferi, Antonio, additional, Tosi, Alberto, additional, Conca, Enrico, additional, Sesta, Vincenzo, additional, Giudice, Andrea, additional, Ruggeri, Alessandro, additional, Tisa, Simone, additional, Flocke, Alexander, additional, Rosinski, Bogdan, additional, Dinten, Jean-Marc, additional, Perriollat, Mathieu, additional, Savery, David, additional, Sportouche, Hélène, additional, Arridge, Simon, additional, Farina, Andrea, additional, Panizza, Pietro, additional, Venturini, Elena, additional, Gordebeke, Peter, additional, Zolda, Pamela, additional, and Taroni, Paola, additional

Published
2020
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24. A Performance Comparison between Continuous-Wave and Time-Domain Diffuse Optical Tomography for Deep Inclusions by Using Small Source-Detector Separations

Author

Orive-Miguel, David, Dinten, Jean-Marc, Mars, Jerome, Condat, Laurent, Hervé, Lionel, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), GIPSA - Signal Images Physique (GIPSA-SIGMAPHY), Département Images et Signal (GIPSA-DIS), Grenoble Images Parole Signal Automatique (GIPSA-lab ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Grenoble Images Parole Signal Automatique (GIPSA-lab ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut Polytechnique de Grenoble - Grenoble Institute of Technology-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), GIPSA - Architecture, Géométrie, Perception, Images, Gestes (GIPSA-AGPIG), GDR ISIS, GDR ONDES, Club Physique et Imagerie Optique de la SFO, and Mars, Jerome

Subjects
[INFO.INFO-IM] Computer Science [cs]/Medical Imaging, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing
Abstract

International audience; Continuous-wave (CW) is the most widely used and commercially available technique for performing biomedical diffuse optics tomography. Nevertheless, CW has been proved to suffer from non-uniqueness problems and large source-detector distances have to be used in order to detect inclusions that are deep from the surface. In order to overcome the former drawback frequency-domain approach has been developed although it also suffers from the latter deficiency. Recently, time domain (TD) diffuse optics has attracted huge interest from the scientific community due to its inherit capacity to associate photons arrival time with the maximum depth that they have reached ; the late arrival photons are the photons that have travelled most and presumably have gone deeper in the tissue. Moreover, not long ago it was proved that in TD there is no need of large source-detector distances for the reconstruction of deep layers of human tissue ; in fact small source-detector separations (s.d.s.) improve the contrast and the absolute number of detected photons. However, no comparison analysis has been done in terms of reconstruction quality when using small s.d.s. In this work finite element method is used to do a computational comparison between CW and TD approaches where special emphasis is put on the quality of reconstructions 1) with an inclusion at deep levels and 2) when using small s.d.s. TD reconstructions were done using Mellin-Laplace (TD-ML) transform which is fast to calculate and stable to noise. For the experiments, synthetic computationally generated data was used which was convolved with a single-photon avalanche diode detector instrumental response function.

Published
2017

28. SOLUS: an innovative multimodal imaging system to improve breast cancer diagnosis through diffuse optics and ultrasounds

Author

Fantini, Sergio, Taroni, Paola, Pifferi, Antonio, Dalla Mora, Alberto, Di Sieno, Laura, Ferocino, Edoardo, Tosi, Alberto, Conca, Enrico, Sesta, Vincenzo, Giudice, Andrea, Ruggeri, Alessandro, Tisa, Simone, Flocke, Alexander, Rosinski, Bogdan, Dinten, Jean-Marc, Perriollat, Mathieu, Fraschini, Christophe, Sportouche, Hélène, Arridge, Simon, Di Sciacca, Giuseppe, Farina, Andrea, Panizza, Pietro, Venturini, Elena, Gordebeke, Peter, Zolda, Pamela, and Taroni, Paola

Published
2021
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34. 3D lens-free time-lapse microscopy for 3D cell culture

Author

Berdeu, Anthony, additional, Momey, Fabien, additional, Laperrousaz, Bastien, additional, Bordy, Thomas, additional, Gidrol, Xavier, additional, Dinten, Jean-Marc, additional, Picollet-D'hahan, Nathalie, additional, and Allier, Cédric, additional

Published
2017
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39. Dynamics of cell and tissue growth acquired by means of 25 mm² to 10 cm² lensfree imaging

Author

Momey, Fabien, Coutard, Jean-Guillaume, Bordy, Thomas, Navarro, Fabrice, Menneteau, Mathilde, Dinten, Jean-Marc, Allier, Cédric, Laboratoire Hubert Curien [Saint Etienne] (LHC), Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), SPIE, Laboratoire Hubert Curien (LHC), and Institut d'Optique Graduate School (IOGS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[INFO.INFO-IM]Computer Science [cs]/Medical Imaging
Abstract

International audience; In this paper, we discuss a new methodology based on lens-free imaging to perform wound healing assay with unprecedented statistics. Our video lens-free microscopy setup is a simple optical system featuring only a CMOS sensor and a semi coherent illumination system. Yet it is a powerful means for the real-time monitoring of cultivated cells. It presents several key advantages, e.g., integration into standard incubator, compatibility with standard cell culture protocol, simplicity and ease of use. It can perform the follow-up in a large field of view (25 mm2) of several crucial parameters during the culture of cells i.e. their motility, their proliferation rate or their death. Consequently the setup can gather large statistics both in space and time. But in the case of tissue growth experiments, the field of view of 25 mm2 remains not sufficient and results can be biased depending on the position of the device with respect to the recipient of the cell culture. Hence, to conduct exhaustive wound healing assay, here we propose to enlarge the field of view up to 10 cm2 through two different approaches. The first method consists in performing a scan of the cell culture by moving the source/sensor couple and then stitch the stack of images. The second is to make an acquisition by scanning with a line scan camera. The two approaches are compared in term of resolution, complexity and acquisition time. Next we have performed acquisitions of wound healing assay (keratinocytes HaCaT) both in real-time (25 mm2) and in final point (10 cm2) to assess the combination of these two complementary modalities. In the future, we aim at combining directly super wide field of view acquisitions (>10 cm2) with real time ability inside the incubator.

Published
2015

40. Etude du vieillisement cutané par spectroscopie de réflectance diffuse appliquée au collagène

Author

Roig, Blandine, Guilbert, Marie, Koenig, Anne, Piot, Olivier, Perraut, François, Manfait, Michel, Jeannesson, Pierre, Dinten, Jean-Marc, Rayet, Béatrice, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Médicaments : Dynamique Intracellulaire et Architecture Nucléaire (MéDIAN), and Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

47. Highlighting the impact of aging on type I collagen: label-free investigation using confocal reflectance microscopy and diffuse reflectance spectroscopy in 3D matrix model

Author

Guilbert, Marie, primary, Roig, Blandine, additional, Terryn, Christine, additional, Garnotel, Roselyne, additional, Jeannesson, Pierre, additional, Sockalingum, Ganesh D., additional, Manfait, Michel, additional, Perraut, François, additional, Dinten, Jean-Marc, additional, Koenig, Anne, additional, and Piot, Olivier, additional

Published
2016
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48. Toward noninvasive assessment of flap viability with time-resolved diffuse optical tomography: a preclinical test on rats

Author

Di Sieno, Laura, primary, Bettega, Georges, additional, Aribert, Marion, additional, Berger, Michel, additional, Hamou, Cynthia, additional, Dalla Mora, Alberto, additional, Puszka, Agathe, additional, Grateau, Henri, additional, Contini, Davide, additional, Hervé, Lionel, additional, Coll, Jean-Luc, additional, Dinten, Jean-Marc, additional, Pifferi, Antonio, additional, and Planat-Chrétien, Anne, additional

Published
2016
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