1. Diversity of microbial community and its metabolic potential for nitrogen and sulfur cycling in sediments of Phu Quoc island, Gulf of Thailand
- Author
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Ngoc Tung Quach, Quoc Viet Nguyen, Douglas J. H. Shyu, Cao Bang Phi, Quyet-Tien Phi, Wen-Jun Li, Hang Thuy Dam, Dinh Man Tran, Heonjoong Kang, Van Thuoc Doan, Thanh Ha Le, Thi Hanh Nguyen Vu, Hoang Ha Chu, Kim Thoa Nguyen, and Quang Huy Nguyen
- Subjects
Geologic Sediments ,Thaumarchaeota ,Nitrogen ,Microbiology ,03 medical and health sciences ,Microbial ecology ,RNA, Ribosomal, 16S ,Media Technology ,Nitrogen cycle ,Environmental Microbiology - Research Paper ,030304 developmental biology ,0303 health sciences ,biology ,Bacteria ,030306 microbiology ,Ecology ,Phylum ,Microbiota ,Planctomycetes ,Biodiversity ,biology.organism_classification ,Thailand ,Archaea ,Microbial population biology ,Proteobacteria ,Sulfur - Abstract
Although Phu Quoc island, Gulf of Thailand possesses diverse marine and coastal ecosystems, biodiversity and metabolic capability of microbial communities remain poorly investigated. The aim of our study was to evaluate the biodiversity and metabolic potential of sediment microbial communities in Phu Quoc island. The marine sediments were collected from three different areas and analyzed by using 16S rRNA gene-based amplicon approach. A total of 1,143,939 reads were clustered at a 97% sequence similarity into 8,331 unique operational taxonomic units, representing 52 phyla. Bacteria and archaea occupied averagely around 86% and 14%, respectively, of the total prokaryotic community. Proteobacteria, Planctomycetes, Chloroflexi, and Thaumarchaeota were the dominant phyla in all sediments, which were involved in nitrogen and sulfur metabolism. Sediments harboring of higher nitrogen sources were found to coincide with increased abundance of archaeal phylum Thaumarchaeota. Predictive functional analysis showed high abundance prokaryotic genes associated with nitrogen cycling including nifA-Z, amoABC, nirA, narBIJ, napA, nxrAB, nrfA-K, nirBD, nirS, nirK, norB-Z, nlnA, ald, and ureA-J, based on taxonomic groups detected by 16S rRNA sequencing. Although the key genes involved in sulfur cycling were found to be at low to undetectable levels, the other genes encoding for sulfur-related biological processes were present, suggesting that alternative pathways may be involved in sulfur cycling at our study site. In conclusion, our study for the first time shed light on diversity of microbial communities in Phu Quoc island. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-021-00481-8.
- Published
- 2020