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133 results on '"Ding, Aixiang"'

12. Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

Author

Li, Shuai, Li, Yaoxuan, Zhang, Shiji, Fang, Haixiao, Huang, Ze, Zhang, Duoteng, Ding, Aixiang, Uvdal, Kajsa, Hu, Zhang-Jun, Huang, Kai, Li, Lin, Li, Shuai, Li, Yaoxuan, Zhang, Shiji, Fang, Haixiao, Huang, Ze, Zhang, Duoteng, Ding, Aixiang, Uvdal, Kajsa, Hu, Zhang-Jun, Huang, Kai, and Li, Lin

Abstract

Temperature homeostasis is critical for cells to perform their physiological functions. Among the diverse methods for temperature detection, fluorescent temperature probes stand out as a proven and effective tool, especially for monitoring temperature in cells and suborganelles, with a specific emphasis on mitochondria. The utilization of these probes provides a new opportunity to enhance our understanding of the mechanisms and interconnections underlying various physiological activities related to temperature homeostasis. However, the complexity and variability of cells and suborganelles necessitate fluorescent temperature probes with high resolution and sensitivity. To meet the demanding requirements for intracellular/subcellular temperature detection, several strategies have been developed, offering a range of options to address this challenge. This review examines four fundamental temperature-response strategies employed by small molecule and polymer probes, including intramolecular rotation, polarity sensitivity, Forster resonance energy transfer, and structural changes. The primary emphasis was placed on elucidating molecular design and biological applications specific to each type of probe. Furthermore, this review provides an insightful discussion on factors that may affect fluorescent thermometry, providing valuable perspectives for future development in the field. Finally, the review concludes by presenting cutting-edge response strategies and research insights for mitigating biases in temperature sensing. In this review, we primarily summarized four temperature-response strategies. Then, we further analyzed the chemical modifications and biological applications of the probes. Finally, we have provided a prospective on the future development of probes., Funding Agencies|National Natural Science Foundation of China [22077101]; CPSF [GZB20230379]; China Postdoctoral Science Foundation [2023M742919]; XMU; Fundamental Research Funds for the Central Universities; STINT Joint China-Sweden Mobility Project [CH2017-7243]; Carl Tryggers Stiftelse [CTS 23:2433]; Swedish Government Strategic Research Area in Materials Science on Advanced Functional Materials at Linkoeping University [2009 0097]

Published
2024
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17. Rational Design and Biological Application of Hybrid Fluorophores.

Author

Zhang, Shiji, Qu, Yunwei, Zhang, Duoteng, Li, Shuai, Tang, Fang, Ding, Aixiang, Hu, Lei, Zhang, Jin, Wang, Hui, Huang, Kai, and Li, Lin

Subjects
FLUOROPHORES, CYANINES, STAINS & staining (Microscopy), LIFE sciences, OPTICAL materials, CELL anatomy
Abstract

Fluorophores are considered powerful tools for not only enabling the visualization of cell structures, substructures, and biological processes, but also making for the quantitative and qualitative measurement of various analytes in living systems. However, most fluorophores do not meet the diverse requirements for biological applications in terms of their photophysical and biological properties. Hybridization is an important strategy in molecular engineering that provides fluorophores with complementarity and multifunctionality. This review summarizes the basic strategies of hybridization with four classes of fluorophores, including xanthene, cyanine, coumarin, and BODIPY with a focus on their structure‐property relationship (SPR) and biological applications. This review aims to provide rational hybrid ideas for expanding the reservoir of knowledge regarding fluorophores and promoting the development of newly produced fluorophores for applications in the field of life sciences. [ABSTRACT FROM AUTHOR]

Published
2024
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24. 4D Cell‐Condensate Bioprinting

Author

Ding, Aixiang, primary, Lee, Sang Jin, additional, Tang, Rui, additional, Gasvoda, Kaelyn L., additional, He, Felicia, additional, and Alsberg, Eben, additional

Published
2022
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25. Red‐emissive Dual‐state Fluorogenic Probe for Wash‐free Imaging of Lipid Droplets in Living Cells and Fatty Liver Tissues.

Author

Wang, Hui, Zhang, Cuifeng, Hu, Lei, Tang, Fang, Wang, Yaxuan, Ding, Feiyang, Lu, Jingwen, and Ding, Aixiang

Subjects
FATTY liver, ADIPOSE tissues, NON-alcoholic fatty liver disease, LIVER cells, FLUORESCENT probes
Abstract

Lipid droplet (LD) dysfunction can result in various diseases, such as nonalcoholic fatty liver disease. Imaging agents built on dual‐state emission (DSE) molecules that fluoresce in both dilute solutions and the aggregated state are receiving attention as this type of probe could provide bright fluorescence signals at variable concentrations, avoiding false signal readout caused by the concentration fluctuation in living systems. Herein, we identified a red emissive molecule featuring DSE, from three newly synthesized molecules, for specific detection of LDs in live cells. The bioimaging abilities have been well confirmed by optical spectroscopies, theoretical calculations, cell experiments, as well as animal studies. The DSE probe is effective for LD detection at concentrations ranging from 1 μM to 100 μM while retaining high brightness and signal fidelity. This study provides a knowledge base for the future design of DSE‐active fluorescent probes for understanding LD‐related diseases. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Jammed Micro-Flake Hydrogel for 4D Living Cell Bioprinting

Author

Ding, Aixiang, Jeon, Oju, Cleveland, David, Gasvoda, Kaelyn, Wells, Derrick, Lee, Sang Jin, and Alsberg, Eben

Subjects
Tissue Engineering, Tissue Scaffolds, Printing, Three-Dimensional, Bioprinting, Hydrogels, Article
Abstract

Four-dimensional (4D) bioprinting is promising to build cell-laden constructs (bioconstructs) with complex geometries and functions for tissue/organ regeneration applications. The development of hydrogel-based 4D bioinks, especially those allowing living cell printing, with easy preparation, defined composition, and controlled physical properties is critically important for 4D bioprinting. Here, a single-component jammed micro-flake hydrogel (MFH) system with heterogeneous size distribution, which differs from the conventional granular microgel, has been developed as a new cell-laden bioink for 4D bioprinting. This jammed cytocompatible MFH features scalable production and straightforward composition with shear-thinning, shear-yielding, and rapid self-healing properties. As such, it can be smoothly printed into stable 3D bioconstructs, which can be further crosslinked to form a gradient in crosslinking density when a photoinitiator and a UV absorber are incorporated. After being subject to shape morphing, a variety of complex bioconstructs with well-defined configurations and high cell viability were obtained. Based on this system, 4D cartilage-like tissue formation was demonstrated as a proof-of-concept. The establishment of this versatile new 4D bioink system may open up a number of applications in tissue engineering.

Published
2022

37. Induction of 4D spatiotemporal geometric transformations in high cell density tissues via shape changing hydrogels

Author

Lee, Yu Bin, Jeon, Oju, Lee, Sang Jin, Ding, Aixiang, Wells, Derrick, and Alsberg, Eben

Subjects
sense organs, Article
Abstract

Developing and healing tissues begin as a cellular condensation. Spatiotemporal changes in tissue geometry, transformations in the spatial distribution of the cells and extracellular matrix, are essential for its evolution into a functional tissue. 4D materials, 3D materials capable of geometric changes, may have the potential to recreate the aforementioned biological phenomenon. However, most reported 4D materials are non-degradable and/or not biocompatible, which limits their application in regenerative medicine, and to date there are no systems controlling the geometry of high density cellular condensations and differentiation. Here, we describe 4D high cell density tissues based on shape-changing hydrogels. By sequential photocrosslinking of oxidized and methacrylated alginate (OMA) and methacrylated gelatin (GelMA), bi-layered hydrogels presenting controllable geometric changes without any external stimuli were fabricated. Fibroblasts and human adipose-derived stem cells (ASCs) were incorporated at concentrations up to 1.0 × 10(8) cells/mL to the 4D constructs, and controllable shape changes were achieved in concert with ASCs differentiated down chondrogenic and osteogenic lineages. Bioprinting of the high density cell-laden OMA and GelMA permitted the formation of more complex constructs with defined 4D geometric changes, which may further expand the promise of this approach in regenerative medicine applications.

Published
2021

45. Complex-Formation-Enhanced Fluorescence Quenching Effect for Efficient Detection of Picric Acid

Author

Ding, Aixiang, Yang, Longmei, Zhang, Yuyang, Zhang, Gaobin, Kong, Lin, Zhang, Xuanjun, Tian, Yupeng, Tao, Xutang, Yang, Jiaxiang, Ding, Aixiang, Yang, Longmei, Zhang, Yuyang, Zhang, Gaobin, Kong, Lin, Zhang, Xuanjun, Tian, Yupeng, Tao, Xutang, and Yang, Jiaxiang

Abstract

Amine-functionalized alpha-cyanostilbene derivatives (Z)-2-(4-aminophenyl)-3-(4-butoxyphenyl) acrylonitrile (ABA) and (Z)-3-(4-butoxyphenyl)-2-[4-(butylamino) phenyl] acrylonitrile (BBA) were designed for specific recognition of picric acid (PA), an environmental and biological pollutant. The 1:1 host-guest complexes formed between the chemosensors and PA enhanced fluorescence quenching, thus leading to sensitive and selective detection in aqueous media and the solid phase., Funding Agencies|National Natural Science Foundation of China [21101001, 50873001, 61107014]; Natural Science Foundation of Anhui Province [1208085MB21]; 211 Project of Anhui University; Team for Scientific Innovation Foundation of Anhui Province [2006KJ007TD]; Swedish government (SFO-Mat-Liu) [2009-00971]

Published
2014
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