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δ13C and δ18O values from sapwood of a singlePinus uncinatatree, from a high elevation site in the Spanish Pyrenees, were determined to evaluate the differences between whole wood and resin-free whole wood. This issue is addressed for the first time withP. uncinataover a 38-year long period. Results are also compared with published isotope values of α-cellulose samples from the same tree. The differences in δ13C and δ18O between whole wood and resin-free whole wood vary within the analytical uncertainty of 0.3 and 0.5 ‰, respectively, indicating that resin extraction is not necessary for sapwood ofP. uncinata. Mean differences between cellulose and whole wood are 0.9 ‰ (δ13C) and 5.0 ‰ (δ18O), respectively. However, further analyses of different species and other sites are needed to evaluate whether the findings reported here are coherent more generally. [ABSTRACT FROM PUBLISHER]