15 results on '"Dina Zhu"'
Search Results
2. Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice
- Author
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Chao Chen, Dina Zhu, Shuai Zhang, and Wensheng Zhang
- Subjects
Catechin ,Diabetic nephropathy ,CircRNA ,miRNA ,ceRNA ,Nutrition. Foods and food supply ,TX341-641 - Abstract
(+)-Catechin (CE) is mainly found in green and black tea and has many biological effects, such as anti-inflammatory, anti-cancer, anti-viral effects, protecting human organs, especially the kidney. This study aims to identify the circRNAs induced by CE in db/db mice and their roles in diabetic nephropathy progression. After the db/db mice were treated with CE, RNA-seq was performed to identify the differentially expressed circRNA and mRNAs. The ceRNA regulatory network was constructed and analyzed using bioinformatics software and public databases (Cytoscape, ClueGO, MiRWalk, STRING, et al.). Our results revealed that 6 differentially expressed circRNAs are most associated with the cholinergic synapse, neurotrophin signaling pathway, and insulin signaling pathway. Among these, circRNA.5549 and circRNA.4712 might regulate Cd36, Cyp26b1, C8a, Cyp2j13, Grem2 genes through ceRNA regulatory mechanism in the presence of CE treatment. The expanded network of proteins interacting with these 5 genes shows that the TGF-β signaling pathway, signaling pathways regulating pluripotency of stem cell, fat digestion and absorption, and PPAR signaling pathway was highly enriched. Overall, circRNA.5549 and circRNA.4712 exhibit a promotive function in CE-treated db/db mice, especially in circRNA.5549/miR-29a-5P/Cd36 regulatory network, and this evidence suggest that their ceRNA regulatory network might be a therapeutic target for DN in humans.
- Published
- 2022
- Full Text
- View/download PDF
3. Compound Heterozygosity for KLF1 Mutations Causing Hemolytic Anemia in Children: A Case Report and Literature Review
- Author
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Linlin Xu, Dina Zhu, Yanxia Zhang, Guanxia Liang, Min Liang, Xiaofeng Wei, Xiaoqing Feng, Xuedong Wu, and Xuan Shang
- Subjects
KLF1 ,compound heterozygote ,hereditary hemolytic anemia ,children ,chronic non-spherocytic hemolytic anemia ,β-thalassemia ,Genetics ,QH426-470 - Abstract
BackgroundAnemia is one of the most common diseases affecting children worldwide. Hereditary forms of anemia due to gene mutations are difficult to diagnose because they only rely on clinical manifestations. In regions with high prevalence of thalassemia such as southern China, pediatric patients with a hereditary hemolytic anemia (HHA) phenotype are often diagnosed with β-thalassemia. However, HHA can be caused by other gene defects. Here, a case previously diagnosed with thalassemia in a local hospital was sent to our laboratory for further genetic diagnosis. Preliminary molecular testing did not identify any mutations in globin genes.MethodsAll blood samples were collected after informed consent had been obtain from the proband’s parents. Both clinical and genetic analyses were conducted for the patient and her family members, including clinical data collection and sequencing of the KLF1 gene. Relevant literature was reviewed, including genetically confirmed cases with well-documented clinical summaries.ResultsBased on the detailed clinical data for this case, we diagnosed the patient with severe HHA. Sanger sequencing confirmed that there was a mutation on each KLF1 allele in the proband, which is missense mutation c.892G > C (p.Ala298Pro) inherited from father and frameshift mutation c.525_526insCGGCGCC (p.Gly176Argfs∗179) from the mother, respectively. A summary of the KLF1 mutation spectrum and a clarification of genotype–phenotype correlation were performed through a combined analysis of the case and literature studies.ConclusionThis study corrected the misdiagnosis and identified the etiology in a Chinese patient with HHA. Identification of the disease-causing gene is important for the treatment and care of the patient and prevention of another affected childbirth in her family. In addition, this study provided insight to better distinguish HHA patients with β-thalassemia mutations from those with KLF1 mutations.
- Published
- 2021
- Full Text
- View/download PDF
4. Investigation of the mechanism of copy number variations involving the α-globin gene cluster on chromosome 16: two case reports and literature review
- Author
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Dina Zhu, Linlin Xu, Yanxia Zhang, Guanxia Liang, Xiaofeng Wei, Liyan Li, Wangjie Jin, and Xuan Shang
- Subjects
Genetics ,General Medicine ,Molecular Biology - Abstract
Thalassemia is one of the most common single-gene disorder worldwide. An important genetic cause of thalassemia is copy number variations (CNVs) in the α-globin gene cluster. However, there is no unified summary and discussion on the detailed information and mechanisms of these CNVs. In this study, two novel CNVs, a tandem duplication (αααα
- Published
- 2022
5. Identification of haemolytic anaemia caused by glucose phosphate isomerase deficiency in a thalassaemia-endemic region: Correction of misdiagnosis and consideration of the cause of misdiagnosis
- Author
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Dina Zhu, Guanxia Liang, Yanxia Zhang, Xiaofeng Wei, Xuedong Wu, and Xuan Shang
- Subjects
Hematology - Published
- 2022
6. A novel splicing mutation of ANK1 is associated with phenotypic heterogeneity of hereditary spherocytosis in a Chinese family
- Author
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Linlin Xu, Xiaofeng Wei, Guanxia Liang, Dina Zhu, Yanxia Zhang, Yang Zhang, and Xuan Shang
- Subjects
Ankyrins ,China ,Phenotype ,Asian People ,RNA Splicing ,Mutation ,Molecular Medicine ,Humans ,Mutant Proteins ,Molecular Biology - Abstract
Hereditary spherocytosis (HS) is a common hematological genetic disorder that results in anemia, jaundice and splenomegaly. It is caused by mutations in the ANK1, SPTA, SPTB, SLC4A1 and EPB42 genes, which encode red blood cell membrane and skeletal proteins. Patients show high heterogeneity in phenotype and genotype and the genotype-phenotype correlation still requires clarification. Here, a novel splicing mutation (ANK1: c.4391-2 AC) was identified by whole-exome sequencing (WES) and Sanger sequencing in a Chinese boy who exhibited a moderately severe HS phenotype. However, his father exhibited a mild phenotype, despite carrying the same HS-causing mutation. The function of the mutant ANK1 protein was analyzed by both bioinformatics and experimental analysis. The mutant protein (p.N1463Kfs*4) showed a different 3D-structure and altered subcellular localization, when compared with the wild-type ANK1 protein. These changes disrupted the normal cell membrane structure and resulted in spheroidized red blood cells. Amplification of cDNA from the son and his father revealed a difference in expression of the abnormal transcript produced by the splicing mutation. We proposed that the lower expression of the mutant allele may have contributed to the relatively mild symptoms of the father. Our study verified ANK1 c. c.4391-2 AC as a novel pathogenic mutation that causes HS. We have also provided new insights into the interpretation of phenotypic variability within families, which could greatly improve the clinical diagnosis and genetic counseling of HS.
- Published
- 2022
7. Compound Heterozygosity for
- Author
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Linlin, Xu, Dina, Zhu, Yanxia, Zhang, Guanxia, Liang, Min, Liang, Xiaofeng, Wei, Xiaoqing, Feng, Xuedong, Wu, and Xuan, Shang
- Subjects
KLF1 ,hereditary hemolytic anemia ,children ,chronic non-spherocytic hemolytic anemia ,β-thalassemia ,Genetics ,compound heterozygote ,Original Research - Abstract
Background Anemia is one of the most common diseases affecting children worldwide. Hereditary forms of anemia due to gene mutations are difficult to diagnose because they only rely on clinical manifestations. In regions with high prevalence of thalassemia such as southern China, pediatric patients with a hereditary hemolytic anemia (HHA) phenotype are often diagnosed with β-thalassemia. However, HHA can be caused by other gene defects. Here, a case previously diagnosed with thalassemia in a local hospital was sent to our laboratory for further genetic diagnosis. Preliminary molecular testing did not identify any mutations in globin genes. Methods All blood samples were collected after informed consent had been obtain from the proband’s parents. Both clinical and genetic analyses were conducted for the patient and her family members, including clinical data collection and sequencing of the KLF1 gene. Relevant literature was reviewed, including genetically confirmed cases with well-documented clinical summaries. Results Based on the detailed clinical data for this case, we diagnosed the patient with severe HHA. Sanger sequencing confirmed that there was a mutation on each KLF1 allele in the proband, which is missense mutation c.892G > C (p.Ala298Pro) inherited from father and frameshift mutation c.525_526insCGGCGCC (p.Gly176Argfs∗179) from the mother, respectively. A summary of the KLF1 mutation spectrum and a clarification of genotype–phenotype correlation were performed through a combined analysis of the case and literature studies. Conclusion This study corrected the misdiagnosis and identified the etiology in a Chinese patient with HHA. Identification of the disease-causing gene is important for the treatment and care of the patient and prevention of another affected childbirth in her family. In addition, this study provided insight to better distinguish HHA patients with β-thalassemia mutations from those with KLF1 mutations.
- Published
- 2021
8. Characterization of circRNA-Associated-ceRNA Networks in a Senescence-Accelerated Mouse Prone 8 Brain
- Author
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Dina Zhu, Hejian Li, Wensheng Zhang, Hong Li, Chengqiang Feng, and Shuai Zhang
- Subjects
0301 basic medicine ,Senescence ,Axon terminus ,Gene regulatory network ,Mice, Transgenic ,Biology ,Bioinformatics ,Mice ,03 medical and health sciences ,Alzheimer Disease ,Memory ,RNA interference ,Drug Discovery ,microRNA ,Genetics ,medicine ,Animals ,Cluster Analysis ,Gene Regulatory Networks ,RNA, Messenger ,Maze Learning ,Molecular Biology ,Genetic Association Studies ,Pharmacology ,Competing endogenous RNA ,Gene Expression Profiling ,Brain ,Computational Biology ,RNA, Circular ,medicine.disease ,Gene expression profiling ,Disease Models, Animal ,MicroRNAs ,Gene Ontology ,030104 developmental biology ,Gene Expression Regulation ,RNA ,Molecular Medicine ,Original Article ,RNA Interference ,Alzheimer's disease ,Neuroscience - Abstract
Alzheimer's disease (AD) is one of the most common neurodegenerative diseases. Although many researchers have attempted to explain the origins of AD, developing an effective strategy in AD clinical therapy is difficult. Recent studies have revealed a potential link between AD and circRNA-associated-ceRNA networks. However, few genome-wide studies have identified the potential circRNA-associated-ceRNA pairs involved in AD. In this study, we systematically explored the circRNA-associated-ceRNA mechanism in a 7-month-old senescence-accelerated mouse prone 8 (SAMP8) model brain through deep RNA sequencing. We obtained 235 significantly dysregulated circRNA transcripts, 30 significantly dysregulated miRNAs, and 1,202 significantly dysregulated mRNAs. We then constructed the most comprehensive circRNA-associated-ceRNA networks in SAMP8 brain. GO analysis revealed that these networks were involved in regulating the development of AD from various angles, for instance, axon terminus (GO: 0043679) and synapse (GO: 0045202). Following rigorous selection, we discovered that the circRNA-associated-ceRNA networks in this AD mouse model were mainly involved in the regulation of Aβ clearance (Hmgb2) and myelin function (Dio2). This research is the first to provide a systematic dissection of circRNA-associated-ceRNA profiling in SAMP8 mouse brain. The selected circRNA-associated-ceRNA networks can profoundly affect the diagnosis and therapy of AD in the future.
- Published
- 2017
9. Pharmacokinetic Characteristics of Steamed Notoginseng by an Efficient LC-MS/MS Method for Simultaneously Quantifying Twenty-three Triterpenoids
- Author
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Zhaoqi Dong, Dina Zhu, Shiming Li, Wensheng Zhang, Qile Zhou, Dong Li, and Hong Li
- Subjects
Male ,Analyte ,Panax notoginseng ,01 natural sciences ,Rats, Sprague-Dawley ,03 medical and health sciences ,Plasma ,0302 clinical medicine ,Triterpenoid ,Pharmacokinetics ,Tandem Mass Spectrometry ,Lc ms ms ,Animals ,Chromatography, High Pressure Liquid ,Chromatography ,biology ,Chemistry ,Plant Extracts ,010401 analytical chemistry ,General Chemistry ,biology.organism_classification ,Triterpenes ,0104 chemical sciences ,Rats ,030220 oncology & carcinogenesis ,General Agricultural and Biological Sciences - Abstract
Steamed Panax notoginseng (SNG) has been widely used as a restorative medicine instead of the raw one, but its pharmacokinetic profile is entirely unknown. To address this, we've developed an LC-MS/MS method with high efficiency and sensitivity for simultaneous quantification of 23 triterpenoids (notoginsenosides Fa, Fc, R1, 20( S)-R2, 20( R)-R2, ginsenosides F4, Rb1, Rg1, Rd, Re, Rb2, 20( S)-Rh1, 20( R)-Rh1, Rh4, R k1, R k3, 20( S)-Rg2, 20( S)-Rg3, 20( R)-Rg3, Rg5, C-K, 20( S)-PPT, 20( S)-PPD) from SNG in rat plasma. This validated approach exhibits great linearity, precision, accuracy, recovery, and stability for all analytes. Furthermore, we, for the first time, applied this method to the pharmacokinetic study of SNG and proposed Rb1, Fa, Rd, R k1, Rg5, R k3, Rh4, and 20( S)-PPD to be suitable pharmacokinetic markers of SNG due to their high exposure levels of systemic plasma. Hence, this developed approach would be a powerful tool for future in vivo investigation of various sources of notoginseng-related samples.
- Published
- 2018
10. Development and validation of a UFLC-MS/MS method for simultaneous quantification of sixty-six saponins and their six aglycones: Application to comparative analysis of red ginseng and white ginseng
- Author
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Ying-Ping Wang, Xiu-Wei Yang, Qi-Le Zhou, Dina Zhu, and Wei Xu
- Subjects
0301 basic medicine ,Spectrometry, Mass, Electrospray Ionization ,Ginsenosides ,Electrospray ionization ,Clinical Biochemistry ,Pharmaceutical Science ,Panax ,Tandem mass spectrometry ,01 natural sciences ,Analytical Chemistry ,03 medical and health sciences ,Ginseng ,Chemical marker ,Tandem Mass Spectrometry ,Drug Discovery ,Ultra fast ,Spectroscopy ,Chromatography ,Chemistry ,010401 analytical chemistry ,Reproducibility of Results ,Saponins ,0104 chemical sciences ,Triple quadrupole mass spectrometer ,030104 developmental biology ,Chromatography, Liquid - Abstract
A new and sensitive ultra fast liquid chromatography coupled with electrospray ionization triple quadrupole tandem mass spectrometry (UFLC–MS/MS) method was developed to evaluate the quality of Red ginseng (RG) and to find out its chemical markers by comparing with multi-batches of RG and white ginseng (WG). This innovative method could quantify sixty-six saponins and their six aglycones including 10 pairs of 20(S) and 20(R) epimers within 35 min simultaneously. All compounds could be determined in individual multiple-reaction monitoring channel without interference, and the optimized method was rapid, accurate, precise, reproducible and efficient. Using the orthogonal partial least squared discriminant analysis, ginsenosides Rg5, Rh4, Rk1, Rs4, F4, and 20(S)-Rg3 were found to be the characteristic components of RG, the six compounds should be suggested as quality control markers to distinguish RG from WG. These findings will be significant for standardizing the processing procedures of RG and ensuring the consistent quality, as well as consequently the efficacy of RG in clinical applications. Results will be helpful in providing crucial chemical profiles of RG.
- Published
- 2018
11. Analyses of mRNA Profiling through RNA Sequencing on a SAMP8 Mouse Model in Response to Ginsenoside Rg1 and Rb1 Treatment
- Author
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Dina Zhu, Haijing Zhang, Wensheng Zhang, Chengqiang Feng, Shuai Zhang, and Hong Li
- Subjects
0301 basic medicine ,Genetics ,Pharmacology ,SAMP8 ,Messenger RNA ,Ginsenoside Rg1 ,Mechanism (biology) ,Ginsenoside Rb1 ,RNA ,Morris water navigation task ,RNA sequencing ,Computational biology ,Biology ,Alzheimer's disease ,Genome ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Pharmacology (medical) ,KEGG ,Signal transduction ,Protein kinase A ,030217 neurology & neurosurgery ,Original Research ,gene-level - Abstract
Ginsenoside Rg1 and Rb1 are the major ingredients in two medicines called QiShengLi (Z20027165) and QiShengJing (Z20027164) approved by China. These ingredients are believed to mitigate forgetfulness. Numerous studies have confirmed that GRg1 and GRb1 offer protection against Alzheimer’s disease (AD), and our morris water maze experiment also indicated that GRg1 and GRb1 may attenuate memory deficits in the seven-month-old SAMP8 mice; however, comprehensive understanding of their roles in AD remains limited. This study systematically explored the mechanism at the genome level of the anti-AD effects of GRg1 and GRb1 in a senescence-accelerated mouse prone 8 (SAMP8) model through deep RNA sequencing. A total of 74,885 mRNA transcripts were obtained. Expression analysis showed that 1,780 mRNA transcripts were differentially expressed in SAMP8 mice compared with the SAMP8+GRg1 mice. Moreover, 1,066 significantly dysregulated mRNA transcripts were identified between SAMP8 and SAMP8+GRb1 mice. Analyses according to gene ontology and the Kyoto Encyclopedia of Genes and Genomes revealed that oral administration of GRg1 and GRb1 improved the learning performance of the SAMP8 mouse model from various aspects, such as nervous system development and mitogen-activated protein kinase signaling pathway. The most probable AD-related transcriptional responses after medication were predicted and discussed in detail. This study is the first to provide a systematic dissection of mRNA profiling in SAMP8 mouse brain in response to GRg1 and GRb1 treatment. We explained their efficacy thoroughly from the source (gene-level explanation). The findings serve as a theoretical basis for the exploration of GRg1 and GRb1 as functional drugs with anti-AD activity.
- Published
- 2017
12. Simultaneous quantification of twenty-one ginsenosides and their three aglycones in rat plasma by a developed UFLC-MS/MS assay: Application to a pharmacokinetic study of red ginseng
- Author
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Qi-Le Zhou, Dina Zhu, Xiu-Wei Yang, Wei Xu, and Yan-Fang Yang
- Subjects
Male ,Sapogenins ,Ginsenosides ,Electrospray ionization ,Clinical Biochemistry ,Saponin ,Pharmaceutical Science ,Panax ,Tandem mass spectrometry ,01 natural sciences ,Analytical Chemistry ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Ginseng ,Plasma ,0302 clinical medicine ,Pharmacokinetics ,Tandem Mass Spectrometry ,Drug Discovery ,Animals ,Spectroscopy ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Chromatography ,010401 analytical chemistry ,Selected reaction monitoring ,Saponins ,0104 chemical sciences ,Triple quadrupole mass spectrometer ,Rats ,chemistry ,Ginsenoside ,030220 oncology & carcinogenesis ,Drugs, Chinese Herbal - Abstract
To track the pharmacokinetic features of red ginseng (RG), a rapid and sensitive ultra fast liquid chromatographic coupled with electrospray ionization triple quadrupole tandem mass spectrometry (UFLC-MS/MS) method was developed for simultaneous quantification of twenty-one ginsenosides and their three aglycones, including 18 prototype compounds (ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1, Rg5, Rh4, Rk1, Rk3, 20(S)-Rf, 20(S)-Rg2, 20(R)-Rg2, 20(S)-Rg3, 20(R)-Rg3, 20(S)-Rh1, 20(R)-Rh1, 20(S)-NG-R2), and 6 metabolites (ginsenosides 20(S)-Rh2 and Rh3, 20(S)-protopanaxadiol (PPD), 20(S)-protopanaxatriol (PPT), 20(R)-PPT, ginseng saponin compound K) of RG in rat plasma after oral administration of RG water extract at a single dose of 4g/kg body weight to rats. All analytes with internal standard (digoxin) were detected by multiple reaction monitoring in negative ionization mode and separated on an ACQUITY UPLC® BEH RP-C18 column (1.7μm, 100×2.1mm). This established method was well validated in terms of linearity, sensitivity, intra- and inter-day precisions, accuracy, recovery, matrix effect, stability, and had a lower limit of quantification at the concentration range of 0.12-8.12ng/mL for all of analytes. This UFLC-MS/MS approach was successfully applied to the pharmacokinetic study for RG water extract in rats. We firstly proposed that Rb1, Rb2, Rc, Rd, Rg1, Rg5, 20(S)-Rg3, 20(S)-Rh2, and 20(S)-PPD measured in rat plasma were suitable pharmacokinetic markers of RG extract in rats due to their high systemic exposure levels. Thus, this specific and reliable method will be useful for future applications to pharmacokinetic studies for various sources of ginsenoside samples and Panax herbs in vivo.
- Published
- 2016
13. Inhibition of Advanced Glycation End Product Formation by Pu-erh Tea Ameliorates Progression of Experimental Diabetic Nephropathy
- Author
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Yan-Fang Yang, Jun Sheng, Pei-Ping Shen, Wensheng Zhang, Zhi Li, Wenfeng Xin, Lei Wang, Shijun Yan, Dina Zhu, Shao-Chen Guo, Xiao Cong, and Tao Ma
- Subjects
Glycation End Products, Advanced ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Kidney Glomerulus ,Down-Regulation ,Camellia sinensis ,Diabetic nephropathy ,Mice ,chemistry.chemical_compound ,Lactoylglutathione lyase ,Glycation ,Internal medicine ,medicine ,Animals ,Humans ,Diabetic Nephropathies ,Creatinine ,Tea ,biology ,Plant Extracts ,Insulin ,Methylglyoxal ,General Chemistry ,medicine.disease ,Endocrinology ,chemistry ,Disease Progression ,biology.protein ,Advanced glycation end-product ,Female ,General Agricultural and Biological Sciences ,Rosiglitazone ,medicine.drug - Abstract
Accumulation of advanced glycation end products (AGEs) has been implicated in the development of diabetic nephropathy. We investigated the effects of Pu-erh tea on AGE accumulation associated with diabetic nephropathy. Although it did not affect blood glucose levels and insulin sensitivy, Pu-erh tea treatment for 8 weeks attenuated the increases in urinary albumin, serum creatinine, and mesangial matrix in db/db mice. We found that Pu-erh tea prevented diabetes-induced accumulation of AGEs and led to a decreased level of receptor for AGE expression in glomeruli. Both production and clearance of carbonyl compounds, the main precursor of AGE formation, were probably attenuated by Pu-erh tea in vivo independent of glyoxalase I expression. In vitro, HPLC assay demonstrated Pu-erh tea could trap methylglyoxal in a dose-dependent manner. Our study raises the possibility that inhibition of AGE formation by carbonyl trapping is a promising approach to prevent or arrest the progression of diabetic complications.
- Published
- 2012
14. (+)-Catechin ameliorates diabetic nephropathy by trapping methylglyoxal in type 2 diabetic mice
- Author
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Shijun Yan, Qile Zhou, Jun Sheng, Zhi Li, Dina Zhu, Lei Wang, and Wensheng Zhang
- Subjects
Glycation End Products, Advanced ,Male ,medicine.medical_specialty ,Metabolite ,Interleukin-1beta ,Down-Regulation ,Inflammation ,Catechin ,Proinflammatory cytokine ,Nephropathy ,Cell Line ,Diabetes Mellitus, Experimental ,Diabetic nephropathy ,chemistry.chemical_compound ,Mice ,Glycation ,In vivo ,Internal medicine ,medicine ,Animals ,Humans ,Diabetic Nephropathies ,Phosphorylation ,Tumor Necrosis Factor-alpha ,Methylglyoxal ,Transcription Factor RelA ,medicine.disease ,Pyruvaldehyde ,Mice, Inbred C57BL ,Endocrinology ,chemistry ,Disease Progression ,medicine.symptom ,Food Science ,Biotechnology - Abstract
cope Accumulation of glycolytic metabolite methylglyoxal (MG) in diabetic kidney is thought to contribute to the pathogenesis of nephropathy, either as a direct toxin or as a precursor for advanced glycation end products (AGEs). Using (+)-catechin (CE), a novel MG trapper, we investigated whether MG trapping is sufficient to prevent the progression of diabetic nephropathy in type 2 diabetic mice. Methods and results CE markedly trapped exogenous MG in a time- and dose-dependent manner and formed mono-MG-CE and di-MG-CE adducts, which were characterized by HPLC-ESI-Q-TOFMS. In vivo, CE administration for 16 wk significantly ameliorated renal dysfunction in type 2 diabetic db/db mice, partially due to MG trapping, which in turn inhibited AGEs formation and lowered proinflammatory cytokines, including tumor necrosis factor α and IL-1β. Similarly, the MG trapping and cellular signaling inhibition effects of CE were observed in human endothelium-derived cells under high glucose conditions. Conclusion CE might ameliorate renal dysfunction in diabetic mice as consequences of inhibiting AGEs formation and cutting off inflammatory pathway via MG trapping. Thus, CE may be a potential natural product as an MG scavenger against diabetes-related complications.
- Published
- 2014
15. Analyses of mRNA Profiling through RNA Sequencing on a SAMP8 Mouse Model in Response to Ginsenoside Rg1 and Rb1 Treatment.
- Author
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Shuai Zhang, Dina Zhu, Hong Li, Haijing Zhang, Chengqiang Feng, and Wensheng Zhang
- Subjects
MESSENGER RNA ,RNA sequencing - Abstract
Ginsenoside Rg1 and Rb1 are the major ingredients in two medicines called QiShengLi (Z20027165) and QiShengJing (Z20027164) approved by China. These ingredients are believed to mitigate forgetfulness. Numerous studies have confirmed that GRg1 and GRb1 offer protection against Alzheimer's disease (AD), and our morris water maze (MWM) experiment also indicated that GRg1 and GRb1may attenuatememory deficits in the 7-month-old SAMP8 mice; however, comprehensive understanding of their roles in AD remains limited. This study systematically explored the mechanism at the genome level of the anti-AD effects of GRg1 and GRb1 in a senescence-accelerated mouse prone 8 (SAMP8) model through deep RNA sequencing. A total of 74,885 mRNA transcripts were obtained. Expression analysis showed that 1,780 mRNA transcripts were differentially expressed in SAMP8 mice compared with the SAMP8+GRg1 mice. Moreover, 1,066 significantly dysregulated mRNA transcripts were identified between SAMP8 and SAMP8+GRb1 mice. Analyses according to gene ontology and the Kyoto Encyclopedia of Genes and Genomes revealed that oral administration of GRg1 and GRb1 improved the learning performance of the SAMP8 mouse model from various aspects, such as nervous system development and mitogen-activated protein kinase signaling pathway. The most probable AD-related transcriptional responses after medication were predicted and discussed in detail. This study is the first to provide a systematic dissection of mRNA profiling in SAMP8 mouse brain in response to GRg1 and GRb1 treatment. We explained their efficacy thoroughly from the source (gene-level explanation). The findings serve as a theoretical basis for the exploration of GRg1 and GRb1 as functional drugs with anti-AD activity. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
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