33 results on '"Dik, Andre"'
Search Results
2. Neuronal Mitochondrial Calcium Uniporter (MCU) Deficiency Is Neuroprotective in Hyperexcitability by Modulation of Metabolic Pathways and ROS Balance
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Bierhansl, Laura, Gola, Lukas, Narayanan, Venu, Dik, Andre, Meuth, Sven G., Wiendl, Heinz, and Kovac, Stjepana
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- 2024
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3. Translational imaging of TSPO reveals pronounced innate inflammation in human and murine CD8 T cell–mediated limbic encephalitis
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Gallus, Marco, Roll, Wolfgang, Dik, Andre, Barca, Cristina, Zinnhardt, Bastian, Hicking, Gordon, Mueller, Christoph, Naik, Venu Narayanan, Anstötz, Max, Krämer, Julia, Rolfes, Leoni, Wachsmuth, Lydia, Pitsch, Julika, van Loo, Karen MJ, Räuber, Saskia, Okada, Hideho, Wimberley, Catriona, Strippel, Christine, Golombeck, Kristin S, Johnen, Andreas, Kovac, Stjepana, Groß, Catharina C, Backhaus, Philipp, Seifert, Robert, Lewerenz, Jan, Surges, Rainer, Elger, Christian E, Wiendl, Heinz, Ruck, Tobias, Becker, Albert J, Faber, Cornelius, Jacobs, Andreas H, Bauer, Jan, Meuth, Sven G, Schäfers, Michael, and Melzer, Nico
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Biomedical and Clinical Sciences ,Immunology ,Neurosciences ,Biomedical Imaging ,Clinical Research ,2.1 Biological and endogenous factors ,Aetiology ,Animals ,Humans ,Mice ,Carrier Proteins ,Inflammation ,Limbic Encephalitis ,Positron-Emission Tomography ,Receptors ,GABA - Abstract
Autoimmune limbic encephalitis (ALE) presents with new-onset mesial temporal lobe seizures, progressive memory disturbance, and other behavioral and cognitive changes. CD8 T cells are considered to play a key role in those cases where autoantibodies (ABs) target intracellular antigens or no ABs were found. Assessment of such patients presents a clinical challenge, and novel noninvasive imaging biomarkers are urgently needed. Here, we demonstrate that visualization of the translocator protein (TSPO) with [18F]DPA-714-PET-MRI reveals pronounced microglia activation and reactive gliosis in the hippocampus and amygdala of patients suspected with CD8 T cell ALE, which correlates with FLAIR-MRI and EEG alterations. Back-translation into a preclinical mouse model of neuronal antigen-specific CD8 T cell-mediated ALE allowed us to corroborate our preliminary clinical findings. These translational data underline the potential of [18F]DPA-714-PET-MRI as a clinical molecular imaging method for the direct assessment of innate immunity in CD8 T cell-mediated ALE.
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- 2023
4. [18F]DPA-714-PET-MRI reveals pronounced innate immunity in human anti-LGI1 and anti-CASPR2 limbic encephalitis.
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Roll, Wolfgang, Bauer, Jan, Dik, Andre, Mueller, Christoph, Backhaus, Philipp, Räuber, Saskia, Zinnhardt, Bastian, Gallus, Marco, Wimberley, Catriona, Körtvelyessy, Peter, Schindler, Philipp, Stenzel, Werner, Elger, Christian E., Becker, Albert, Lewerenz, Jan, Wiendl, Heinz, Meuth, Sven G., Schäfers, Michael, and Melzer, Nico
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EPILEPSY ,ANTI-NMDA receptor encephalitis ,NATURAL immunity ,ENCEPHALITIS ,SCIENCE education - Abstract
This document, published in the Journal of Neurology, explores the use of [18F]DPA-714-PET-MRI as a diagnostic tool for autoimmune limbic encephalitis (ALE) with specific autoantibodies. The study involved two patients with these autoantibodies, and the results showed increased tracer uptake in certain areas of the brain. The study suggests that [18F]DPA-714-PET-MRI could be a valuable tool for diagnosing ALE and assessing the immune response in the brain. However, further research is needed to fully understand its potential for clinical use in ALE. [Extracted from the article]
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- 2024
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5. Cerebrospinal fluid proteomics indicates immune dysregulation and neuronal dysfunction in antibody associated autoimmune encephalitis
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Räuber, Saskia, Schroeter, Christina B., Strippel, Christine, Nelke, Christopher, Ruland, Tillmann, Dik, Andre, Golombeck, Kristin S., Regner-Nelke, Liesa, Paunovic, Manuela, Esser, Daniela, Münch, Christian, Rosenow, Felix, van Duijn, Martijn, Henes, Antonia, Ruck, Tobias, Amit, Ido, Leypoldt, Frank, Titulaer, Maarten J., Wiendl, Heinz, Meuth, Sven G., Meyer zu Hörste, Gerd, Melzer, Nico, Räuber, Saskia, Schroeter, Christina B., Strippel, Christine, Nelke, Christopher, Ruland, Tillmann, Dik, Andre, Golombeck, Kristin S., Regner-Nelke, Liesa, Paunovic, Manuela, Esser, Daniela, Münch, Christian, Rosenow, Felix, van Duijn, Martijn, Henes, Antonia, Ruck, Tobias, Amit, Ido, Leypoldt, Frank, Titulaer, Maarten J., Wiendl, Heinz, Meuth, Sven G., Meyer zu Hörste, Gerd, and Melzer, Nico
- Abstract
Autoimmune Encephalitis (AE) spans a group of non-infectious inflammatory conditions of the central nervous system due to an imbalanced immune response. Aiming to elucidate the pathophysiological mechanisms of AE, we applied an unsupervised proteomic approach to analyze the cerebrospinal fluid (CSF) protein profile of AE patients with autoantibodies against N-methyl-D-aspartate receptor (NMDAR) (n = 9), leucine-rich glioma-inactivated protein 1 (LGI1) (n = 9), or glutamate decarboxylase 65 (GAD65) (n = 8) compared to 9 patients with relapsing-remitting multiple sclerosis as inflammatory controls, and 10 patients with somatic symptom disorder as non-inflammatory controls. We found a dysregulation of the complement system, a disbalance between pro-inflammatory and anti-inflammatory proteins on the one hand, and dysregulation of proteins involved in synaptic transmission, synaptogenesis, brain connectivity, and neurodegeneration on the other hand to a different extent in all AE subtypes compared to non-inflammatory controls. Furthermore, elevated levels of several proteases and reduction in protease inhibitors could be detected in all AE subtypes compared to non-inflammatory controls. Moreover, the different AE subtypes showed distinct protein profiles compared to each other and inflammatory controls which may facilitate future identification of disease-specific biomarkers. Overall, CSF proteomics provides insights into the complex pathophysiological mechanisms of AE, including immune dysregulation, neuronal dysfunction, neurodegeneration, and altered protease function.
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- 2023
6. Myelination- and immune-mediated MR-based brain network correlates
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Cerina, Manuela, Muthuraman, Muthuraman, Gallus, Marco, Koirala, Nabin, Dik, Andre, Wachsmuth, Lydia, Hundehege, Petra, Schiffler, Patrick, Tenberge, Jan-Gerd, Fleischer, Vinzenz, Gonzalez-Escamilla, Gabriel, Narayanan, Venu, Krämer, Julia, Faber, Cornelius, Budde, Thomas, Groppa, Sergiu, and Meuth, Sven G.
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- 2020
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7. Cerebrospinal fluid proteomics indicates immune dysregulation and neuronal dysfunction in antibody associated autoimmune encephalitis
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Räuber, Saskia, primary, Schroeter, Christina B., additional, Strippel, Christine, additional, Nelke, Christopher, additional, Ruland, Tillmann, additional, Dik, Andre, additional, Golombeck, Kristin S., additional, Regner-Nelke, Liesa, additional, Paunovic, Manuela, additional, Esser, Daniela, additional, Münch, Christian, additional, Rosenow, Felix, additional, van Duijn, Martijn, additional, Henes, Antonia, additional, Ruck, Tobias, additional, Amit, Ido, additional, Leypoldt, Frank, additional, Titulaer, Maarten J., additional, Wiendl, Heinz, additional, Meuth, Sven G., additional, Meyer zu Hörste, Gerd, additional, and Melzer, Nico, additional
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- 2023
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8. Management and prognostic markers in patients with autoimmune encephalitis requiring ICU treatment
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Schubert, Julia, Brämer, Dirk, Huttner, Hagen B., Gerner, Stefan T., Fuhrer, Hannah, Melzer, Nico, Dik, Andre, Prüss, Harald, Ly, Lam-Than, Fuchs, Kornelius, Leypoldt, Frank, Nissen, Gunnar, Schirotzek, Ingo, Dohmen, Christian, Bösel, Julian, Lewerenz, Jan, Thaler, Franziska, Kraft, Andrea, Juranek, Aleksandra, Ringelstein, Marius, Sühs, Kurt-Wolfram, Urbanek, Christian, Scherag, André, Geis, Christian, Witte, Otto W., and Günther, Albrecht
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- 2019
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9. Amygdala enlargement and emotional responses in (autoimmune) temporal lobe epilepsy
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Holtmann, Olga, Schlossmacher, Insa, Moenig, Constanze, Johnen, Andreas, Rutter, Lisa-Marie, Tenberge, Jan-Gerd, Schiffler, Patrick, Everding, Judith, Golombeck, Kristin S., Strippel, Christine, Dik, Andre, Schwindt, Wolfram, Wiendl, Heinz, Meuth, Sven G., Bruchmann, Maximilian, Melzer, Nico, and Straube, Thomas
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- 2018
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10. genome-wide association study in autoimmune neurological syndromes with anti-GAD65 autoantibodies.
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Strippel, Christine, Herrera-Rivero, Marisol, Wendorff, Mareike, Tietz, Anja K, Degenhardt, Frauke, Witten, Anika, Schroeter, Christina, Nelke, Christopher, Golombeck, Kristin S, Madlener, Marie, Rüber, Theodor, Ernst, Leon, Racz, Attila, Baumgartner, Tobias, Widman, Guido, Doppler, Kathrin, Thaler, Franziska, Siebenbrodt, Kai, Dik, Andre, and Kerin, Constanze
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GENOME-wide association studies ,GLUTAMATE decarboxylase ,LOCUS (Genetics) ,AUTOANTIBODIES ,PROTEIN kinase C ,EPILEPSY - Abstract
Autoimmune neurological syndromes (AINS) with autoantibodies against the 65 kDa isoform of the glutamic acid decarboxylase (GAD65) present with limbic encephalitis, including temporal lobe seizures or epilepsy, cerebellitis with ataxia, and stiff-person-syndrome or overlap forms. Anti-GAD65 autoantibodies are also detected in autoimmune diabetes mellitus, which has a strong genetic susceptibility conferred by human leukocyte antigen (HLA) and non-HLA genomic regions. We investigated the genetic predisposition in patients with anti-GAD65 AINS. We performed a genome-wide association study (GWAS) and an association analysis of the HLA region in a large German cohort of 1214 individuals. These included 167 patients with anti-GAD65 AINS, recruited by the German Network for Research on Autoimmune Encephalitis (GENERATE), and 1047 individuals without neurological or endocrine disease as population-based controls. Predictions of protein expression changes based on GWAS findings were further explored and validated in the CSF proteome of a virtually independent cohort of 10 patients with GAD65-AINS and 10 controls. Our GWAS identified 16 genome-wide significant (P < 5 × 10
−8 ) loci for the susceptibility to anti-GAD65 AINS. The top variant, rs2535288 [ P = 4.42 × 10−16 , odds ratio (OR) = 0.26, 95% confidence interval (CI) = 0.187–0.358], localized to an intergenic segment in the middle of the HLA class I region. The great majority of variants in these loci (>90%) mapped to non-coding regions of the genome. Over 40% of the variants have known regulatory functions on the expression of 48 genes in disease relevant cells and tissues, mainly CD4+ T cells and the cerebral cortex. The annotation of epigenomic marks suggested specificity for neural and immune cells. A network analysis of the implicated protein-coding genes highlighted the role of protein kinase C beta (PRKCB) and identified an enrichment of numerous biological pathways participating in immunity and neural function. Analysis of the classical HLA alleles and haplotypes showed no genome-wide significant associations. The strongest associations were found for the DQA1*03:01-DQB1*03:02-DRB1*04:01HLA haplotype (P = 4.39 × 10−4 , OR = 2.5, 95%CI = 1.499–4.157) and DRB1*04:01 allele (P = 8.3 × 10−5 , OR = 2.4, 95%CI = 1.548–3.682) identified in our cohort. As predicted, the CSF proteome showed differential levels of five proteins (HLA-A/B, C4A, ATG4D and NEO1) of expression quantitative trait loci genes from our GWAS in the CSF proteome of anti-GAD65 AINS. These findings suggest a strong genetic predisposition with direct functional implications for immunity and neural function in anti-GAD65 AINS, mainly conferred by genomic regions outside the classical HLA alleles. [ABSTRACT FROM AUTHOR]- Published
- 2023
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11. Neuropsychological Performance in Autoimmune Limbic Encephalitis: Evidence from an Immunotherapy-Naïve Cohort
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Mueller, Christoph, primary, Langenbruch, Lisa, additional, Rau, Johanna M H, additional, Brix, Tobias, additional, Strippel, Christine, additional, Dik, Andre, additional, Golombeck, Kristin S, additional, Mönig, Constanze, additional, Johnen, Andreas, additional, Räuber, Saskia, additional, Wiendl, Heinz, additional, Meuth, Sven G, additional, Bölte, Jens, additional, Kovac, Stjepana, additional, and Melzer, Nico, additional
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- 2022
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12. Impact of T cells on neurodegeneration in anti‐GAD65 limbic encephalitis
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Dik, Andre, primary, Widman, Guido, additional, Schulte‐Mecklenbeck, Andreas, additional, Witt, Juri‐Alexander, additional, Pitsch, Julika, additional, Golombeck, Kristin S., additional, Wagner, Jan, additional, Gallus, Marco, additional, Strippel, Christine, additional, Hansen, Niels, additional, Mönig, Constanze, additional, Räuber, Saskia, additional, Wiendl, Heinz, additional, Elger, Christian E., additional, Surges, Rainer, additional, Meuth, Sven G., additional, Helmstaedter, Christoph, additional, Gross, Catharina C., additional, Becker, Albert J., additional, and Melzer, Nico, additional
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- 2021
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13. Determinants of cognition in autoimmune limbic encephalitis—A retrospective cohort study
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Mueller, Christoph, primary, Langenbruch, Lisa M., additional, Rau, Johanna M. H., additional, Brix, Tobias, additional, Strippel, Christine, additional, Dik, Andre, additional, Golombeck, Kristin S., additional, Moenig, Constanze, additional, Raeuber, Saskia J., additional, Kovac, Stjepana, additional, Wiendl, Heinz, additional, Meuth, Sven G., additional, Bölte, Jens, additional, Johnen, Andreas, additional, and Melzer, Nico, additional
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- 2021
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14. Cd8+ t-lymphocyte–driven limbic encephalitis results in temporal lobe epilepsy
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Pitsch, Julika, van Loo, Karen M. J., Naik, Venu Narayanan, Wachsmuth, Lydia, Faber, Cornelius, Surges, Rainer, Kurts, Christian, Schoch, Susanne, Melzer, Nico, Becker, Albert J., Gallus, Marco, Dik, Andre, Kamalizade, Delara, Baumgart, Ann-Kathrin, Gnatkovsky, Vadym, Müller, Johannes Alexander, Opitz, Thoralf, and Hicking, Gordon
- Abstract
Annals of neurology 89(4), 666-685 (2021). doi:10.1002/ana.26000, Published by Wiley-Blackwell, Hoboken, NJ
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- 2021
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15. CD8+ T‐Lymphocyte–Driven Limbic Encephalitis Results in Temporal Lobe Epilepsy
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Pitsch, Julika, primary, van Loo, Karen M. J., additional, Gallus, Marco, additional, Dik, Andre, additional, Kamalizade, Delara, additional, Baumgart, Ann‐Kathrin, additional, Gnatkovsky, Vadym, additional, Müller, Johannes Alexander, additional, Opitz, Thoralf, additional, Hicking, Gordon, additional, Naik, Venu Narayanan, additional, Wachsmuth, Lydia, additional, Faber, Cornelius, additional, Surges, Rainer, additional, Kurts, Christian, additional, Schoch, Susanne, additional, Melzer, Nico, additional, and Becker, Albert J., additional
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- 2021
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16. Additional file 5 of Myelination- and immune-mediated MR-based brain network correlates
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Cerina, Manuela, Muthuraman, Muthuraman, Gallus, Marco, Nabin Koirala, Dik, Andre, Wachsmuth, Lydia, Hundehege, Petra, Schiffler, Patrick, Jan-Gerd Tenberge, Fleischer, Vinzenz, Gonzalez-Escamilla, Gabriel, Venu Narayanan, Krämer, Julia, Faber, Cornelius, Budde, Thomas, Groppa, Sergiu, and Meuth, Sven G.
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mental disorders - Abstract
Additional file 5: Figure S5. The amyloid precursor protein (APP) accumulation upon de- and remyelination in frontal neocortical regions. Exemplary pictures show APP staining in the lower layers of frontal neocortical regions in control conditions (left), at 6 weeks after starting the cuprizone diet (cupri 6 weeks – full demyelination, middle), and at full remyelination 6 weeks after reintroduction of normal food (right). Note that an increase of positive cells, indicating an accumulation of APP in the neuronal soma, occurred slowly at the onset of the cuprizone diet to reach a significant threshold at remyelinating phases. On the right, bar graphs show the number of APP positive cells (cells/mm2) in Cx.
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- 2020
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17. Additional file 6 of Myelination- and immune-mediated MR-based brain network correlates
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Cerina, Manuela, Muthuraman, Muthuraman, Gallus, Marco, Nabin Koirala, Dik, Andre, Wachsmuth, Lydia, Hundehege, Petra, Schiffler, Patrick, Jan-Gerd Tenberge, Fleischer, Vinzenz, Gonzalez-Escamilla, Gabriel, Venu Narayanan, Krämer, Julia, Faber, Cornelius, Budde, Thomas, Groppa, Sergiu, and Meuth, Sven G.
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Additional file 6: Figure S6. Exploratory and grooming behavior were altered by cuprizone diet and its withdrawn. (a) Bar graph showing quantification of vertical exploratory behavior. Animals show a significant increase in comparison to control 2- and 6 weeks after the beginning of the diet. (b) Bar graph showing the quantification of grooming behavior. The latter is often considered a indicator of stress levels I rodents and here it is significantly decreased, in comparison to control, in almost all experimental groups. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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- 2020
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18. Additional file 2 of Myelination- and immune-mediated MR-based brain network correlates
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Cerina, Manuela, Muthuraman, Muthuraman, Gallus, Marco, Nabin Koirala, Dik, Andre, Wachsmuth, Lydia, Hundehege, Petra, Schiffler, Patrick, Jan-Gerd Tenberge, Fleischer, Vinzenz, Gonzalez-Escamilla, Gabriel, Venu Narayanan, Krämer, Julia, Faber, Cornelius, Budde, Thomas, Groppa, Sergiu, and Meuth, Sven G.
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Additional file 2: Figure S2. Representation of technical and biological replicates for histological evaluation of PLP intensity, astrocytosis and microglial activation in the Cx. (a) Scatter plots and graphs show the variability of the data acquired and used for histological evaluation on myelin intensity by using the specific marker PLP in the cortex (upper panel). (b) Scatter plots and graphs show the variability of the data acquired and used for histological evaluation of the number of astrocytes by using the specific marker GFAP in the cortex (mid panel). Simplified bar graphs are shown in the main figures.
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- 2020
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19. Additional file 3 of Myelination- and immune-mediated MR-based brain network correlates
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Cerina, Manuela, Muthuraman, Muthuraman, Gallus, Marco, Nabin Koirala, Dik, Andre, Wachsmuth, Lydia, Hundehege, Petra, Schiffler, Patrick, Jan-Gerd Tenberge, Fleischer, Vinzenz, Gonzalez-Escamilla, Gabriel, Venu Narayanan, Krämer, Julia, Faber, Cornelius, Budde, Thomas, Groppa, Sergiu, and Meuth, Sven G.
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Additional file 3: Figure S3. Structural and anatomical thalamic grey matter changes during de- and remyelination in the cuprizone model. (a) Exemplary pictures show staining for the specific myelin marker PLP in coronal mouse slices containing the ventrobasal complex of the thalamus (VB) in control conditions (left), at 6 weeks after starting the cuprizone diet (cupri 6 weeks – full demyelination, middle), and at full remyelination 6 weeks after reintroduction of normal food (right). Note the decreased signal for PLP indicating demyelination in the cupri 6 weeks group in comparison to control, and a persistent low PLP signal during remyelination. On the right, bar graphs show the quantification of myelin loss and regain for all groups and all investigated time points. (b) Exemplary pictures show staining for the specific astrocytic marker GFAP in coronal mouse slices containing the ventrobasal complex of the thalamus in control conditions (left), at 6 weeks after starting the cuprizone diet (cupri 6 weeks – full demyelination, middle), and at full remyelination 6 weeks after reintroduction of normal food (right). Note the increased number of astrocytes indicating astrocytosis in the cupri 6 weeks group in comparison to control and remy 6 weeks groups. On the right, bar graphs show the number of astrocytes (cells/mm2) in VB, this increased according to diet progression and cuprizone withdrawal.
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- 2020
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20. Additional file 4 of Myelination- and immune-mediated MR-based brain network correlates
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Cerina, Manuela, Muthuraman, Muthuraman, Gallus, Marco, Nabin Koirala, Dik, Andre, Wachsmuth, Lydia, Hundehege, Petra, Schiffler, Patrick, Jan-Gerd Tenberge, Fleischer, Vinzenz, Gonzalez-Escamilla, Gabriel, Venu Narayanan, Krämer, Julia, Faber, Cornelius, Budde, Thomas, Groppa, Sergiu, and Meuth, Sven G.
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nervous system - Abstract
Additional file 4: Figure S4. Representation of technical and biological replicates for histological evaluation of PLP intensity, astrocytosis and microglial activation in the thalamus. (a) Scatter plots and graphs show the variability of the data acquired and used for histological evaluation on myelin intensity by using the specific marker PLP in the thalamus (upper panel). (b) Scatter plots and graphs show the variability of the data acquired and used for histological evaluation of the number of astrocytes by using the specific marker GFAP in the thalamus (mid panel).
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- 2020
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21. Additional file 1 of Myelination- and immune-mediated MR-based brain network correlates
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Cerina, Manuela, Muthuraman, Muthuraman, Gallus, Marco, Nabin Koirala, Dik, Andre, Wachsmuth, Lydia, Hundehege, Petra, Schiffler, Patrick, Jan-Gerd Tenberge, Fleischer, Vinzenz, Gonzalez-Escamilla, Gabriel, Venu Narayanan, Krämer, Julia, Faber, Cornelius, Budde, Thomas, Groppa, Sergiu, and Meuth, Sven G.
- Abstract
Additional file 1: Figure S1. Representation of technical and biological replicates for histological evaluation of PLP intensity, astrocytosis and microglial activation in the CC. (a) Scatter plots and graphs show the variability of the data acquired and used for histological evaluation on myelin intensity by using the specific marker PLP in the corpus callosum (upper panel). (b) Scatter plots and graphs show the variability of the data acquired and used for histological evaluation of the number of astrocytes by using the specific marker GFAP in the corpus callosum (mid panel). Simplified bar graphs are shown in the main figures.
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- 2020
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22. Rituximab Treatment and Long-term Outcome of Patients With Autoimmune Encephalitis
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Thaler, Franziska S, Zimmermann, Luise, Urbanek, Christian, Pfefferkorn, Thomas, Philipsen, Alexandra, Piepgras, Johannes, von Podewils, Felix, Priller, Josef, Pröbstel, Anne-Katrin, Helena Rau, Johanna Maria, Räuber, Saskia Jania, Reimann, Gernot, Reinecke, Raphael, Sommer, Claudia, Ringelstein, Marius, Rohner, Hendrik, Rosenow, Felix, Rostásy, Kevin, Rüegg, Stephan, Schaumberg, Jens, Schmidt, Jens, Schmütz, Ina-Isabelle, Schreiber, Stephan, Schreyer, Gesa, Doppler, Kathrin, Schröder, Ina, Schuster, Simon, Seidel, Günter, Senel, Makbule, Siebenbrodt, Kai, Stammel, Oliver, Stangel, Martin, Stolze, Henning, Stoppe, Muriel, Storm Van's Gravesande, Karin, Penner, Loana, Sybre, Steffen, Tauber, Simone, Bergh, Florian Then, Trebst, Corinna, Trendelenburg, George, Trollmann, Regina, Tumani, Hayrettin, Türedi, Methab, von Mering, Matthias, Wagner, Judith, Lewerenz, Jan, Weissert, Robert, Wiendl, Heinz, Wildemann, Brigitte, Witt, Karsten, Wöpking, Sigrid, Wunderlich, Benjamin, Zieger, Lara, Roessling, Rosa, Finke, Carsten, Prüß, Harald, Melzer, Nico, Wandinger, Klaus-Peter, Kammermeier, Stefan, Leypoldt, Frank, Kümpfel, Tania, Encephalitis, German Network for Research on Autoimmune, Adelmann, Michael, Appeltshauser, Luise, Ayzenberg, Ilya, Baade-Büttner, Carolin, van Baalen, Andreas, Baatz, Sebastian, Balint, Bettina, Strippel, Christine, Bauer, Sebastian, Baumgartner, Annette, Benesch, Sonka, Berger, Robert, Berning, Sascha, Bernsen, Sarah, Bien, Christian, Bien, Corinna, Binder, Andreas, Bittner, Stefan, Bittner, Daniel, Blaes, Franz, Blaschek, Astrid, Dargvainiene, Justina, Dik, Andre, Dreesmann, Mona, Ebinger, Friedrich, Edelhoff, Lena, Ehrlich, Sven, Eisenhut, Katharina, Kraft, Andrea, Endres, Dominique, Entscheva, Marina, Faiss, Jürgen Hartmut, Fazeli, Walid Ahmad, Finke, Alexander, Fitzner, Dirk, Flotats-Bastardas, Marina, Paul, Friedemann, Friese, Manuel, Gallus, Marco, Sühs, Kurt-Wolfram, Gebhard, Marcel, Geis, Christian, Gorsler, Anna, Grau, Armin, Grauer, Oliver, Groß, Catharina, Gül, Halime, Handreka, Robert, Hansen, Niels, Häusler, Martin, Wickel, Jonathan, Havla, Joachim, Ha-Yeun, Chung, Heide, Wolfgang, Held, Valentin, Hellwig, Kerstin, Hillebrand, Philip, Hoffmann, Frank, Oy, Ulrich Hofstadt-van, Ismail, Fatme Seval, Jansen, Martina, Kaufmann, Max, Kellinghaus, Christoph, Knake, Susanne, Körtvelyessy, Peter, Kovac, Stjepana, Krämer, Markus, Krogias, Christos, Lehrich, Christoph, Linsa, Andreas, Lünemann, Jan, Markewitz, Robert, Malter, Michael, Melzer, Kristin Stefanie, Menge, Til, Meuth, Sven, Meyer Zu Hörste, Gerd, Mönig, Constanze, Mono, Marie-Luise, Nagel, Michael, Neumann-Haefelin, Tobias, and Obrocki, Jost
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Adult ,Male ,medicine.medical_specialty ,immunology [Anti-N-Methyl-D-Aspartate Receptor Encephalitis] ,anti-GAD65 autoantibody ,Disease ,Real world evidence ,Gastroenterology ,immunology [Autoimmune Diseases of the Nervous System] ,pharmacology [Immunosuppressive Agents] ,anti-CASPR2 autoantibody ,administration & dosage [Rituximab] ,Modified Rankin Scale ,Internal medicine ,Outcome Assessment, Health Care ,immunology [Autoantibodies] ,Humans ,Medicine ,In patient ,ddc:610 ,Registries ,immunology [Encephalitis] ,anti-NMDA receptor autoantibody ,Aged ,Autoantibodies ,Autoimmune encephalitis ,pharmacology [Rituximab] ,biology ,business.industry ,Middle Aged ,drug therapy [Encephalitis] ,Neurology ,Cohort ,administration & dosage [Immunosuppressive Agents] ,anti-leucine-rich glioma-inactivated 1 autoantibody ,biology.protein ,drug therapy [Autoimmune Diseases of the Nervous System] ,Female ,Rituximab ,Neurology (clinical) ,Antibody ,drug therapy [Anti-N-Methyl-D-Aspartate Receptor Encephalitis] ,business ,Immunosuppressive Agents ,Follow-Up Studies ,medicine.drug - Abstract
Background and ObjectivesTo determine the real-world use of rituximab in autoimmune encephalitis (AE) and to correlate rituximab treatment with the long-term outcome.MethodsPatients with NMDA receptor (NMDAR)-AE, leucine-rich glioma-inactivated-1 (LGI1)- AE, contactin-associated protein-like-2 (CASPR2)-AE, or glutamic acid decarboxylase 65 (GAD65) disease from the GErman Network for Research on AuToimmune Encephalitis who had received at least 1 rituximab dose and a control cohort of non–rituximab-treated patients were analyzed retrospectively.ResultsOf the 358 patients, 163 (46%) received rituximab (NMDAR-AE: 57%, CASPR2-AE: 44%, LGI1-AE: 43%, and GAD65 disease: 37%). Rituximab treatment was initiated significantly earlier in NMDAR- and LGI1-AE (median: 54 and 155 days from disease onset) compared with CASPR2-AE or GAD65 disease (median: 632 and 1,209 days). Modified Rankin Scale (mRS) scores improved significantly in patients with NMDAR-AE, both with and without rituximab treatment. Although being more severely affected at baseline, rituximab-treated patients with NMDAR-AE more frequently reached independent living (mRS score ≤2) (94% vs 88%). In LGI1-AE, rituximab-treated and nontreated patients improved, whereas in CASPR2-AE, only rituximab-treated patients improved significantly. No improvement was observed in patients with GAD65 disease. A significant reduction of the relapse rate was observed in rituximab-treated patients (5% vs 13%). Detection of NMDAR antibodies was significantly associated with mRS score improvement. A favorable outcome was also observed with early treatment initiation.DiscussionWe provide real-world data on immunosuppressive treatments with a focus on rituximab treatment for patients with AE in Germany. We suggest that early and short-term rituximab therapy might be an effective and safe treatment option in most patients with NMDAR-, LGI1-, and CASPR2-AE.Class of EvidenceThis study provides Class IV evidence that rituximab is an effective treatment for some types of AE.
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- 2021
23. Myelination- and immune mediated MR-based brain network correlates
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Cerina, Manuela, primary, Muthuraman, Muthuraman, additional, Gallus, Marco, additional, Koirala, Nabin, additional, Dik, Andre, additional, Wachsmuth, Lydia, additional, Hundehege, Petra, additional, Schiffler, Patrick, additional, Tenberge, Jan-Gerd, additional, Fleischer, Vinzenz, additional, Gonzalez-Escamilla, Gabriel, additional, Narayanan, Venu, additional, Krämer, Julia, additional, Faber, Cornelius, additional, Budde, Thomas, additional, Groppa, Sergiu, additional, and Meuth, Sven G, additional
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- 2020
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24. CD8+ T‐Lymphocyte–Driven Limbic Encephalitis Results in Temporal Lobe Epilepsy.
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Pitsch, Julika, Loo, Karen M. J., Gallus, Marco, Dik, Andre, Kamalizade, Delara, Baumgart, Ann‐Kathrin, Gnatkovsky, Vadym, Müller, Johannes Alexander, Opitz, Thoralf, Hicking, Gordon, Naik, Venu Narayanan, Wachsmuth, Lydia, Faber, Cornelius, Surges, Rainer, Kurts, Christian, Schoch, Susanne, Melzer, Nico, and Becker, Albert J.
- Subjects
TEMPORAL lobe epilepsy ,KILLER cells ,ENCEPHALITIS ,T cells ,LYMPHOCYTE subsets - Abstract
Objective: Limbic encephalitis (LE) comprises a spectrum of inflammatory changes in affected brain structures including the presence of autoantibodies and lymphoid cells. However, the potential of distinct lymphocyte subsets alone to elicit key clinicopathological sequelae of LE potentially inducing temporal lobe epilepsy (TLE) with chronic spontaneous seizures and hippocampal sclerosis (HS) is unresolved. Methods: Here, we scrutinized pathogenic consequences emerging from CD8+ T cells targeting hippocampal neurons by recombinant adeno‐associated virus‐mediated expression of the model‐autoantigen ovalbumin (OVA) in CA1 neurons of OT‐I/RAG1−/− mice (termed "OVA‐CD8+ LE model"). Results: Viral‐mediated antigen transfer caused dense CD8+ T cell infiltrates confined to the hippocampal formation starting on day 5 after virus transduction. Flow cytometry indicated priming of CD8+ T cells in brain‐draining lymph nodes preceding hippocampal invasion. At the acute model stage, the inflammatory process was accompanied by frequent seizure activity and impairment of hippocampal memory skills. Magnetic resonance imaging scans at day 7 of the OVA‐CD8+ LE model revealed hippocampal edema and blood–brain barrier disruption that converted into atrophy until day 40. CD8+ T cells specifically targeted OVA‐expressing, SIINFEKL‐H‐2Kb–positive CA1 neurons and caused segmental apoptotic neurodegeneration, astrogliosis, and microglial activation. At the chronic model stage, mice exhibited spontaneous recurrent seizures and persisting memory deficits, and the sclerotic hippocampus was populated with CD8+ T cells escorted by NK cells. Interpretation: These data indicate that a CD8+ T‐cell–initiated attack of distinct hippocampal neurons is sufficient to induce LE converting into TLE‐HS. Intriguingly, the role of CD8+ T cells exceeds neurotoxic effects and points to their major pathogenic role in TLE following LE. ANN NEUROL 2021;89:666–685 [ABSTRACT FROM AUTHOR]
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- 2021
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25. An Assay to Determine Mechanisms of Rapid Autoantibody-Induced Neurotransmitter Receptor Endocytosis and Vesicular Trafficking in Autoimmune Encephalitis
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Amedonu, Elsie, primary, Brenker, Christoph, additional, Barman, Sumanta, additional, Schreiber, Julian A., additional, Becker, Sebastian, additional, Peischard, Stefan, additional, Strutz-Seebohm, Nathalie, additional, Strippel, Christine, additional, Dik, Andre, additional, Hartung, Hans-Peter, additional, Budde, Thomas, additional, Wiendl, Heinz, additional, Strünker, Timo, additional, Wünsch, Bernhard, additional, Goebels, Norbert, additional, Meuth, Sven G., additional, Seebohm, Guiscard, additional, and Melzer, Nico, additional
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- 2019
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26. Genetic predisposition in anti-LGI1 and anti-NMDA receptor encephalitis
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Mueller, Stefanie H, Färber, Anna, Sühs, Kurt-Wolfram, Lempert, Thomas, Winter, Benjamin, Schluh, Giesela, Gass, Achim, Hagenah, Ulrich, Hausler, Martin Georg, König, Schiffner, C., Tauber, Simone, Ayzenbeig, Ilya, Ringelstein, Marius, Ingo, Kleiter, Hellwig, Kerstin, Krogias, Christos, Heneka, Michael, Widmann, Guido, Witt, Juri-Alexander, Niehusmann, Pitt, Wagner, Jan, Schoch, Susanne, Becker, Albert, Kellinghaus, Christoph, Helmstaedter, Christoph, Elger, Christian, Kehrer, Stefanie, Langenfurth, Anika, Spruth, Eike, Priller, Josef, Paul, Friedemann, Harms, Lutz, Finke, Carsten, Wöpking, Sigrid, Bien, Christian G, Schneider, Auke, Seitz, Rüdiger, Goebels, Norbert, Aktas, Orhan, Huttner, Hagen, Gemer, Stefan, Staykov, Dimitre, van Elst, Ludger Tebartz, Endres, Dominique, Kraft, Andrea, Stich, Oliver, Rauer, Sebastian, Baumgartner, Annette, Neubauer, Bemd, Hofmann, Regina, Schirotzek, Ingo, Döppner, Thorsten, von Podewils, Felix, Süße, M., Dressel, Alexander, Zettl, Uwe K, Kaufmann, Max, Fleischer, Sabine, Heesen, Christoph, Stellmann, Jan-Patrick, Friese, Manuel, Ufer, Frederike, Trebst, Corinna, Stangel, Martin, Schönenberger, Silvia, Ehrlich, Sven, Wildemann, Brigitte, Jarius, Sven, Bösel, Julian, Roepke, Luise, Brämer, Dirk, Strubl, Julia, Kunze, Albrecht, Geis, Christian, Günther, Albrecht, Grote, Susann, Handreka, Robert, Maker, Michael, Dohmen, Christian, Stoppe, Muriel, Bergh, Florian Then, Ettrich, Barbara, Schilling, Ruth, Koertvelyessy, Peter, Bittner, Daniel, Spreer, Annette, Methner, Axel, Rostásy, Kevin, Pledl, Hans-Wemer, Hoyer, Carolin, Anlonso, Angelika, Szabo, Kristina, Held, Valentin, Tackenberg, B., Meuth, Sven, Philipp, Konstanze, Golombeck, Stefanie, Posevitz-Fejfar, Anita, Then Bergh, Florian, Wuerdemann, Till, Dik, Andre, Strippel, Christine, Möning, Constanze, Wiendl, Heinz, Melzer, Nico, Weissert, Robert, Fuchs, Komelius, Retzlaff, Nele, Schroeder, Herr, Prüss, Harald, Faiss, Jürgen H, Zettl, Uwe, Fousse, Mathias, Ragoschke-Schumm, Andreas, van Baalen, Andreas, Stumer, Klarissa, Berg, Daniela, Peuser, Anna, Nissen, Gunnar, Schmütz, Isa, Leypoldt, Frank, Lieb, Wolfgang, Bartsch, Thorsten, Falk, Kim Christin, Hummel, Katja, Moser, Andreas, Wandinger, Klaus Peter, Becker, Felicitas, Krumbholz, Markus, Bischof, Felix, Ihle, Jennifer, Dürr, Marc, Franke, Andre, Tumani, Hayrettin, Lewerenz, Jan, Doppler, Kathrin, Sommer, Claudia, Kuhlenbäumer, Gregor, Wandinger, Klaus-Peter, Encephalitis, German Network for Research on Autoimmune, Krämer, Markus, Lyss, Birgit, Schreiber, Stephan, Faiss, J., Wallenfelsova, Eva, Othman, Jalal, Sinnecker, Tim, Riedlinger, Arne, Fonseca, Patricia, Kern, Peter, Bogarin, Yuri, Hoffmann, Anna, Schwabe, Julia, Golombeck, Kristin S, Seidel, Gunter, Herwig, Antje, Rostasy, Kevin, Stolze, Henning, Körber, Rüdiger, Wiese, Lars, Schulze-Amberger, Jörg, Heide, Wolfgang, Geis, Tobias, Kümpfel, Tania, Blaes, Franz, Köhler, Wolfgang, Dogan, Muejgan, Bien, Christian, Kramme, Corinna, Grofß, Martin, Thomsen, Jens, Pulkowski, Ulrich, Hoepner, Robert, Thaler, Franziska, Schindler, Kaspar, Lippert, Julian, Marziniak, Martin, Ewering, Carina, Reimann, Gemot, Juranek, Aleksandra, Kanitz, Rolf-Dieter, Obrocki, Jost, Pfefferkorn, Thomas, Schimrigk, Sebastian, Elisak, Martin, Urbanek, Christian, Schön, Frauke, Schabet, Martin, Melville, Jutta, Finke, Alexander, Henningsen, Henning, Hansen, Hans-Christian, Küpper, Philipp, Berning, Sascha, Nagel, Michael, Hofstadt, Ulrich, Trendelenburg, George, Heider, Steffen, Linsa, Andreas, Muth, Carolin, Borggräfe, Ingo, Blaschek, Astrid, Laurent, Sarah, Havla, Joachim, Menge, Til, Bieniek, Rolf, Hoffmann, Frank, Philipsen, Alexandra, Gorsler, Anna, Pomschlegel, Sebastian, and Tesch, Manfred
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Adult ,Male ,Adolescent ,genetics [Protein Serine-Threonine Kinases] ,Hashimoto Disease ,Protein Serine-Threonine Kinases ,LGI1 protein, human ,genetics [Protein-Serine-Threonine Kinases] ,Polymorphism, Single Nucleotide ,Receptors, N-Methyl-D-Aspartate ,Young Adult ,Doublecortin-Like Kinases ,immunology [Receptors, N-Methyl-D-Aspartate] ,genetics [Hashimoto Disease] ,Humans ,Genetic Predisposition to Disease ,ddc:610 ,immunology [Encephalitis] ,genetics [Genetic Predisposition to Disease] ,Aged ,Autoantibodies ,metabolism [Hashimoto Disease] ,Intracellular Signaling Peptides and Proteins ,Proteins ,genetics [Encephalitis] ,DCLK2 protein, human ,Middle Aged ,Protein-Serine-Threonine Kinases ,genetics [Proteins] ,immunology [Proteins] ,immunology [Hashimoto Disease] ,genetics [Polymorphism, Single Nucleotide] ,metabolism [Encephalitis] ,Encephalitis ,metabolism [Autoantibodies] ,Female ,Genome-Wide Association Study - Abstract
We performed a genome-wide association study in 1,194 controls and 150 patients with anti-N-methyl-D-aspartate receptor (anti-NMDAR, n = 96) or anti-leucine-rich glioma-inactivated1 (anti-LGI1, n = 54) autoimmune encephalitis. Anti-LGI1 encephalitis was highly associated with 27 single-nucleotide polymorphisms (SNPs) in the HLA-II region (leading SNP rs2858870 p = 1.22 × 10
- Published
- 2017
27. Contradictory effects of erythropoietin on inhibitory synaptic transmission in left and right prelimbic cortex of mice
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Dik, Andre, primary, Saffari, Roja, additional, Zhang, Mingyue, additional, and Zhang, Weiqi, additional
- Published
- 2018
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28. Onconeural antigen spreading in paraneoplastic neurological disease due to small cell lung cancer
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Dik, Andre, primary, Strippel, Christine, additional, Mönig, Constanze, additional, Golombeck, Kristin S, additional, Schulte-Mecklenbeck, Andreas, additional, Wiendl, Heinz, additional, Meuth, Sven G, additional, Johnen, Andreas, additional, Gross, Catharina C, additional, and Melzer, Nico, additional
- Published
- 2018
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29. Treating refractory post-herpetic anti-N-methyl-d-aspartate receptor encephalitis with rituximab
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Strippel, Christine, primary, Mönig, Constanze, additional, Golombeck, Kristin S., additional, Dik, Andre, additional, Bönte, Kathrin, additional, Kovac, Stjepana, additional, Schulte-Mecklenbeck, Andreas, additional, Wiendl, Heinz, additional, Meuth, Sven G., additional, Johnen, Andreas, additional, Gross, Catharina C., additional, and Melzer, Nico, additional
- Published
- 2017
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30. A genome-wide association study in autoimmune neurological syndromes with anti-GAD65 autoantibodies
- Author
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Strippel, Christine, Herrera-Rivero, Marisol, Wendorff, Mareike, Tietz, Anja K., Degenhardt, Frauke, Witten, Anika, Schroeter, Christina, Nelke, Christopher, Golombeck, Kristin S., Madlener, Marie, Rueber, Theodor, Ernst, Leon, Racz, Attila, Baumgartner, Tobias, Widman, Guido, Doppler, Kathrin, Thaler, Franziska, Siebenbrodt, Kai, Dik, Andre, Kerin, Constanze, Rauber, Saskia, Gallus, Marco, Kovac, Stjepana, Grauer, Oliver M., Grimm, Alexander, Pruess, Harald, Wickel, Jonathan, Geis, Christian, Lewerenz, Jan, Goebels, Norbert, Ringelstein, Marius, Menge, Til, Tackenberg, Bjoern, Kellinghaus, Christoph, Bien, Christian G., Kraft, Andrea, Zettl, Uwe, Ismail, Fatme Seval, Ayzenberg, Ilya, Urbanek, Christian, Suhs, Kurt-Wolfram, Tauber, Simone C., Mues, Sigrid, Kortvelyessy, Peter, Markewitz, Robert, Paliantonis, Asterios, Elger, Christian E., Surges, Rainer, Sommer, Claudia, Kumpfel, Tania, Gross, Catharina C., Lerche, Holger, Wellmer, Jorg, Quesada, Carlos M., Bergh, Florian Then, Wandinger, Klaus-Peter, Becker, Albert J., Kunz, Wolfram S., zu Hoerste, Gerd Meyer, Malter, Michael P., Rosenow, Felix, Wiendl, Heinz, Kuhlenbaumer, Gregor, Leypoldt, Frank, Lieb, Wolfgang, Franke, Andre, Meuth, Sven G., Stoll, Monika, Melzer, Nico, Strippel, Christine, Herrera-Rivero, Marisol, Wendorff, Mareike, Tietz, Anja K., Degenhardt, Frauke, Witten, Anika, Schroeter, Christina, Nelke, Christopher, Golombeck, Kristin S., Madlener, Marie, Rueber, Theodor, Ernst, Leon, Racz, Attila, Baumgartner, Tobias, Widman, Guido, Doppler, Kathrin, Thaler, Franziska, Siebenbrodt, Kai, Dik, Andre, Kerin, Constanze, Rauber, Saskia, Gallus, Marco, Kovac, Stjepana, Grauer, Oliver M., Grimm, Alexander, Pruess, Harald, Wickel, Jonathan, Geis, Christian, Lewerenz, Jan, Goebels, Norbert, Ringelstein, Marius, Menge, Til, Tackenberg, Bjoern, Kellinghaus, Christoph, Bien, Christian G., Kraft, Andrea, Zettl, Uwe, Ismail, Fatme Seval, Ayzenberg, Ilya, Urbanek, Christian, Suhs, Kurt-Wolfram, Tauber, Simone C., Mues, Sigrid, Kortvelyessy, Peter, Markewitz, Robert, Paliantonis, Asterios, Elger, Christian E., Surges, Rainer, Sommer, Claudia, Kumpfel, Tania, Gross, Catharina C., Lerche, Holger, Wellmer, Jorg, Quesada, Carlos M., Bergh, Florian Then, Wandinger, Klaus-Peter, Becker, Albert J., Kunz, Wolfram S., zu Hoerste, Gerd Meyer, Malter, Michael P., Rosenow, Felix, Wiendl, Heinz, Kuhlenbaumer, Gregor, Leypoldt, Frank, Lieb, Wolfgang, Franke, Andre, Meuth, Sven G., Stoll, Monika, and Melzer, Nico
- Abstract
Autoimmune neurological syndromes (AINS) with autoantibodies against the 65kDa isoform of the glutamic acid decarboxylase (GAD65) present with limbic encephalitis, including temporal lobe seizures or epilepsy, cerebellitis with ataxia, and stiff-person-syndrome or overlap forms. Anti-GAD65 autoantibodies are also detected in autoimmune diabetes mellitus, which has a strong genetic susceptibility conferred by human leukocyte antigen (HLA) and non-HLA genomic regions. We investigated the genetic predisposition in patients with anti-GAD65 AINS. We performed a genome-wide association study (GWAS) and an association analysis of the HLA region in a large German cohort of 1214 individuals. These included 167 patients with anti-GAD65 AINS, recruited by the German Network for Research on Autoimmune Encephalitis (GENERATE), and 1047 individuals without neurological or endocrine disease as population-based controls. Predictions of protein expression changes based on GWAS findings were further explored and validated in the CSF proteome of a virtually independent cohort of 10 patients with GAD65-AINS and 10 controls. Our GWAS identified 16 genome-wide significant (P < 5 x 10(-8)) loci for the susceptibility to anti-GAD65 AINS. The top variant, rs2535288 [P = 4.42 x 10(-16), odds ratio (OR) = 0.26, 95% confidence interval (CI) = 0.187-0.358], localized to an intergenic segment in the middle of the HLA class I region. The great majority of variants in these loci (>90%) mapped to non-coding regions of the genome. Over 40% of the variants have known regulatory functions on the expression of 48 genes in disease relevant cells and tissues, mainly CD4(+) T cells and the cerebral cortex. The annotation of epigenomic marks suggested specificity for neural and immune cells. A network analysis of the implicated protein-coding genes highlighted the role of protein kinase C beta (PRKCB) and identified an enrichment of numerous biological pathways participating in immunity and neural function
31. A genome-wide association study in autoimmune neurological syndromes with anti-GAD65 autoantibodies.
- Author
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Strippel C, Herrera-Rivero M, Wendorff M, Tietz AK, Degenhardt F, Witten A, Schroeter C, Nelke C, Golombeck KS, Madlener M, Rüber T, Ernst L, Racz A, Baumgartner T, Widman G, Doppler K, Thaler F, Siebenbrodt K, Dik A, Kerin C, Räuber S, Gallus M, Kovac S, Grauer OM, Grimm A, Prüss H, Wickel J, Geis C, Lewerenz J, Goebels N, Ringelstein M, Menge T, Tackenberg B, Kellinghaus C, Bien CG, Kraft A, Zettl U, Ismail FS, Ayzenberg I, Urbanek C, Sühs KW, Tauber SC, Mues S, Körtvélyessy P, Markewitz R, Paliantonis A, Elger CE, Surges R, Sommer C, Kümpfel T, Gross CC, Lerche H, Wellmer J, Quesada CM, Then Bergh F, Wandinger KP, Becker AJ, Kunz WS, Meyer Zu Hörste G, Malter MP, Rosenow F, Wiendl H, Kuhlenbäumer G, Leypoldt F, Lieb W, Franke A, Meuth SG, Stoll M, and Melzer N
- Subjects
- Humans, Proteome genetics, Histocompatibility Antigens Class II, HLA Antigens, Haplotypes, Alleles, Autoantibodies, HLA-DRB1 Chains genetics, Genome-Wide Association Study, Genetic Predisposition to Disease genetics
- Abstract
Autoimmune neurological syndromes (AINS) with autoantibodies against the 65 kDa isoform of the glutamic acid decarboxylase (GAD65) present with limbic encephalitis, including temporal lobe seizures or epilepsy, cerebellitis with ataxia, and stiff-person-syndrome or overlap forms. Anti-GAD65 autoantibodies are also detected in autoimmune diabetes mellitus, which has a strong genetic susceptibility conferred by human leukocyte antigen (HLA) and non-HLA genomic regions. We investigated the genetic predisposition in patients with anti-GAD65 AINS. We performed a genome-wide association study (GWAS) and an association analysis of the HLA region in a large German cohort of 1214 individuals. These included 167 patients with anti-GAD65 AINS, recruited by the German Network for Research on Autoimmune Encephalitis (GENERATE), and 1047 individuals without neurological or endocrine disease as population-based controls. Predictions of protein expression changes based on GWAS findings were further explored and validated in the CSF proteome of a virtually independent cohort of 10 patients with GAD65-AINS and 10 controls. Our GWAS identified 16 genome-wide significant (P < 5 × 10-8) loci for the susceptibility to anti-GAD65 AINS. The top variant, rs2535288 [P = 4.42 × 10-16, odds ratio (OR) = 0.26, 95% confidence interval (CI) = 0.187-0.358], localized to an intergenic segment in the middle of the HLA class I region. The great majority of variants in these loci (>90%) mapped to non-coding regions of the genome. Over 40% of the variants have known regulatory functions on the expression of 48 genes in disease relevant cells and tissues, mainly CD4+ T cells and the cerebral cortex. The annotation of epigenomic marks suggested specificity for neural and immune cells. A network analysis of the implicated protein-coding genes highlighted the role of protein kinase C beta (PRKCB) and identified an enrichment of numerous biological pathways participating in immunity and neural function. Analysis of the classical HLA alleles and haplotypes showed no genome-wide significant associations. The strongest associations were found for the DQA1*03:01-DQB1*03:02-DRB1*04:01HLA haplotype (P = 4.39 × 10-4, OR = 2.5, 95%CI = 1.499-4.157) and DRB1*04:01 allele (P = 8.3 × 10-5, OR = 2.4, 95%CI = 1.548-3.682) identified in our cohort. As predicted, the CSF proteome showed differential levels of five proteins (HLA-A/B, C4A, ATG4D and NEO1) of expression quantitative trait loci genes from our GWAS in the CSF proteome of anti-GAD65 AINS. These findings suggest a strong genetic predisposition with direct functional implications for immunity and neural function in anti-GAD65 AINS, mainly conferred by genomic regions outside the classical HLA alleles., (© The Author(s) 2022. Published by Oxford University Press on behalf of the Guarantors of Brain.)
- Published
- 2023
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- View/download PDF
32. CD8 + T-Lymphocyte-Driven Limbic Encephalitis Results in Temporal Lobe Epilepsy.
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Pitsch J, van Loo KMJ, Gallus M, Dik A, Kamalizade D, Baumgart AK, Gnatkovsky V, Müller JA, Opitz T, Hicking G, Naik VN, Wachsmuth L, Faber C, Surges R, Kurts C, Schoch S, Melzer N, and Becker AJ
- Subjects
- Animals, Blood-Brain Barrier pathology, CA1 Region, Hippocampal pathology, Epilepsy, Temporal Lobe psychology, Hippocampus pathology, Homeodomain Proteins genetics, Limbic Encephalitis psychology, Lymph Nodes pathology, Magnetic Resonance Imaging, Memory Disorders etiology, Memory Disorders psychology, Mice, Inbred C57BL, Mice, Knockout, Neurons pathology, Ovalbumin genetics, Ovalbumin immunology, Peptide Fragments genetics, Seizures genetics, Seizures pathology, Mice, CD8-Positive T-Lymphocytes pathology, Epilepsy, Temporal Lobe etiology, Epilepsy, Temporal Lobe pathology, Limbic Encephalitis complications, Limbic Encephalitis pathology
- Abstract
Objective: Limbic encephalitis (LE) comprises a spectrum of inflammatory changes in affected brain structures including the presence of autoantibodies and lymphoid cells. However, the potential of distinct lymphocyte subsets alone to elicit key clinicopathological sequelae of LE potentially inducing temporal lobe epilepsy (TLE) with chronic spontaneous seizures and hippocampal sclerosis (HS) is unresolved., Methods: Here, we scrutinized pathogenic consequences emerging from CD8
+ T cells targeting hippocampal neurons by recombinant adeno-associated virus-mediated expression of the model-autoantigen ovalbumin (OVA) in CA1 neurons of OT-I/RAG1-/- mice (termed "OVA-CD8+ LE model")., Results: Viral-mediated antigen transfer caused dense CD8+ T cell infiltrates confined to the hippocampal formation starting on day 5 after virus transduction. Flow cytometry indicated priming of CD8+ T cells in brain-draining lymph nodes preceding hippocampal invasion. At the acute model stage, the inflammatory process was accompanied by frequent seizure activity and impairment of hippocampal memory skills. Magnetic resonance imaging scans at day 7 of the OVA-CD8+ LE model revealed hippocampal edema and blood-brain barrier disruption that converted into atrophy until day 40. CD8+ T cells specifically targeted OVA-expressing, SIINFEKL-H-2Kb -positive CA1 neurons and caused segmental apoptotic neurodegeneration, astrogliosis, and microglial activation. At the chronic model stage, mice exhibited spontaneous recurrent seizures and persisting memory deficits, and the sclerotic hippocampus was populated with CD8+ T cells escorted by NK cells., Interpretation: These data indicate that a CD8+ T-cell-initiated attack of distinct hippocampal neurons is sufficient to induce LE converting into TLE-HS. Intriguingly, the role of CD8+ T cells exceeds neurotoxic effects and points to their major pathogenic role in TLE following LE. ANN NEUROL 2021;89:666-685., (© 2020 The Authors. Annals of Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association.)- Published
- 2021
- Full Text
- View/download PDF
33. Management and prognostic markers in patients with autoimmune encephalitis requiring ICU treatment.
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Schubert J, Brämer D, Huttner HB, Gerner ST, Fuhrer H, Melzer N, Dik A, Prüss H, Ly LT, Fuchs K, Leypoldt F, Nissen G, Schirotzek I, Dohmen C, Bösel J, Lewerenz J, Thaler F, Kraft A, Juranek A, Ringelstein M, Sühs KW, Urbanek C, Scherag A, Geis C, Witte OW, and Günther A
- Subjects
- Adult, Female, Humans, Intensive Care Units statistics & numerical data, Male, Middle Aged, Outcome Assessment, Health Care, Prognosis, Registries, Risk Factors, Young Adult, Critical Care statistics & numerical data, Encephalitis diagnosis, Encephalitis therapy, Hashimoto Disease diagnosis, Hashimoto Disease therapy
- Abstract
Objective: To assess intensive care unit (ICU) complications, their management, and prognostic factors associated with outcomes in a cohort of patients with autoimmune encephalitis (AE)., Methods: This study was an observational multicenter registry of consecutively included patients diagnosed with AE requiring Neuro-ICU treatment between 2004 and 2016 from 18 tertiary hospitals. Logistic regression models explored the influence of complications, their management, and diagnostic findings on the dichotomized (0-3 vs 4-6) modified Rankin Scale score at hospital discharge., Results: Of 120 patients with AE (median age 43 years [interquartile range 24-62]; 70 females), 101 developed disorders of consciousness, 54 autonomic disturbances, 42 status epilepticus, and 39 severe sepsis. Sixty-eight patients were mechanically ventilated, 85 patients had detectable neuronal autoantibodies, and 35 patients were seronegative. Worse neurologic outcome at hospital discharge was associated with necessity of mechanical ventilation (sex- and age-adjusted OR 6.28; 95% CI, 2.71-15.61) tracheostomy (adjusted OR 6.26; 95% CI, 2.68-15.73), tumor (adjusted OR 3.73; 95% CI, 1.35-11.57), sepsis (adjusted OR 4.54; 95% CI, 1.99-10.43), or autonomic dysfunction (adjusted OR 2.91; 95% CI, 1.24-7.3). No significant association was observed with autoantibody type, inflammatory changes in CSF, or pathologic MRI., Conclusion: In patients with AE, mechanical ventilation, sepsis, and autonomic dysregulation appear to indicate longer or incomplete convalescence. Classic ICU complications better serve as prognostic markers than the individual subtype of AE. Increased awareness and effective management of these AE-related complications are warranted, and further prospective studies are needed to confirm our findings and to develop specific strategies for outcome improvement.
- Published
- 2018
- Full Text
- View/download PDF
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