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6. Impaired competence in flagellar mutants of Bacillus subtilis is connected to the regulatory network governed by DegU.:Running title: Hindered competence by lack of motility

Author

Hölscher, Theresa, Schiklang, Tina, Dragos, Anna, Dietel, Anne-Kathrin, Kost, Christian, and Kovács, Ákos T.

Subjects
Flagella, Competence, Viscosity, Phosphorylation, Bacillus subtilis
Abstract

The competent state is a developmentally distinct phase, in which bacteria are able to take up and integrate exogenous DNA into their genome. Bacillus subtilis is one of the naturally competent bacterial species and the domesticated laboratory strain 168 is easily transformable. In this study, we report a reduced transformation frequency of B. subtilis mutants lacking functional and structural flagellar components. This includes hag, the gene encoding the flagellin protein forming the filament of the flagellum. We confirm that the observed decrease of the transformation frequency is due to reduced expression of competence genes, particularly of the main competence regulator comK. The impaired competence is due to an increase in the phosphorylated form of the response regulator DegU, which is involved in regulation of both flagellar motility and competence. Altogether, our study identified a close link between motility and natural competence in B. subtilis suggesting that hindrance in motility has great impact on differentiation of this bacterium not restricted only to the transition towards sessile growth stage.

Published
2018
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9. Impaired competence in flagellar mutants of <italic>Bacillus subtilis</italic> is connected to the regulatory network governed by DegU.

Author

Hölscher, Theresa, Schiklang, Tina, Dragoš, Anna, Dietel, Anne‐Kathrin, Kost, Christian, and Kovács, Ákos T.

Subjects
BACILLUS subtilis genetics, BACTERIAL genomes, FLAGELLIN, PHOSPHORYLATION, BACTERIAL growth
Abstract

Summary: The competent state is a developmentally distinct phase, in which bacteria are able to take up and integrate exogenous DNA into their genome. Bacillus subtilis is one of the naturally competent bacterial species and the domesticated laboratory strain 168 is easily transformable. In this study, we report a reduced transformation frequency of B. subtilis mutants lacking functional and structural flagellar components. This includes hag, the gene encoding the flagellin protein forming the filament of the flagellum. We confirm that the observed decrease of the transformation frequency is due to reduced expression of competence genes, particularly of the main competence regulator gene comK. The impaired competence is due to an increase in the phosphorylated form of the response regulator DegU, which is involved in regulation of both flagellar motility and competence. Altogether, our study identified a close link between motility and natural competence in B. subtilis suggesting that hindrance in motility has great impact on differentiation of this bacterium not restricted only to the transition towards sessile growth stage. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

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