Search

Your search keyword '"Dieguez, Hernán H."' showing total 13 results
Start Over Author "Dieguez, Hernán H."
13 results on '"Dieguez, Hernán H."'

8. Therapeutic benefit of environmental enrichment on optic neuritis

Author

Aranda, Marcos L., primary, González Fleitas, María F., additional, Dieguez, Hernán H., additional, Milne, Georgia A., additional, Devouassoux, Julián D., additional, Keller Sarmiento, María I., additional, Chianelli, Mónica, additional, Sande, Pablo H., additional, Dorfman, Damián, additional, and Rosenstein, Ruth E., additional

Published
2019
Full Text
View/download PDF

12. Superior cervical gangliectomy induces non-exudative age-related macular degeneration in mice

Author

Dieguez, Hernán H., Romeo, Horacio E., González Fleitas, María F., Aranda, Marcos L., Milne, Georgia A., Rosenstein, Ruth E., and Dorfman, Damián

Abstract

Non-exudative age-related macular degeneration, a prevalent cause of blindness, is a progressive and degenerative disease characterized by alterations in Bruch's membrane, retinal pigment epithelium, and photoreceptors exclusively localized in the macula. Although experimental murine models exist, the vast majority take a long time to develop retinal alterations and, in general, these alterations are ubiquitous, with many resulting from non-eye-specific genetic manipulations; additionally, most do not always reproduce the hallmarks of human age-related macular degeneration. Choroid vessels receive sympathetic innervation from the superior cervical ganglion, which, together with the parasympathetic system, regulates blood flow into the choroid. Choroid blood flow changes have been involved in age-related macular degeneration development and progression. At present, no experimental models take this factor into account. The aim of this work was to analyze the effect of superior cervical gangliectomy (also known as ganglionectomy) on the choroid, Bruch's membrane, retinal pigment epithelium and retina. Adult male C57BL/6J mice underwent unilateral superior cervical gangliectomy and a contralateral sham procedure. Although superior cervical gangliectomy induced ubiquitous choroid and choriocapillaris changes, it induced Bruch's membrane thickening, loss of retinal pigment epithelium melanin content and retinoid isomerohydrolase, the appearance of drusen-like deposits, and retinal pigment epithelium and photoreceptor atrophy, exclusively localized in the temporal side. Moreover, superior cervical gangliectomy provoked a localized increase in retinal pigment epithelium and photoreceptor apoptosis, and a decline in photoreceptor electroretinographic function. Therefore, superior cervical gangliectomy recapitulated the main features of human non-exudative age-related macular degeneration, and could become a new experimental model of dry age-related macular degeneration, and a useful platform for developing new therapies.

Published
2018
Full Text
View/download PDF

13. An ethologically relevant paradigm to assess visual contrast sensitivity in rodents.

Author

Calanni JS, Aranda ML, Dieguez HH, Dorfman D, Schmidt TM, and Rosenstein RE

Abstract

In the animal kingdom, threat information is perceived mainly through vision. The subcortical visual pathway plays a critical role in the rapid processing of visual information-induced fear, and triggers a response. Looming-evoked behavior in rodents, mimicking response to aerial predators, allowed identify the neural circuitry underlying instinctive defensive behaviors; however, the influence of disk/background contrast on the looming-induced behavioral response has not been examined, either in rats or mice. We studied the influence of the dark disk/gray background contrast in the type of rat and mouse defensive behavior in the looming arena, and we showed that rat and mouse response as a function of disk/background contrast adjusted to a sigmoid-like relationship. Both sex and age biased the contrast-dependent response, which was dampened in rats submitted to retinal unilateral or bilateral ischemia. Moreover, using genetically manipulated mice, we showed that the three type of photoresponsive retinal cells (i.e., cones, rods, and intrinsically photoresponsive retinal ganglion cells (ipRGCs)), participate in the contrast-dependent response, following this hierarchy: cones ˃> rods ˃>>ipRGCs. The cone and rod involvement was confirmed using a mouse model of unilateral non-exudative age-related macular degeneration, which only damages canonical photoreceptors and significantly decreased the contrast sensitivity in the looming arena.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results