52 results on '"Diego Zappa"'
Search Results
2. The Impact of Reinsurance Strategies on Capital Requirements for Premium Risk in Insurance
- Author
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Gian Paolo Clemente, Nino Savelli, and Diego Zappa
- Subjects
capital requirement for premium risk ,collective risk model ,reinsurance strategies ,Solvency II ,Insurance ,HG8011-9999 - Abstract
New risk-based solvency requirements for insurance companies across European markets have been introduced by Solvency II and will come in force from 1 January 2016. These requirements, derived by a Standard Formula or an Internal Model, will be by far more risk-sensitive than the required solvency margin provided by the current legislation. In this regard, a Partial Internal Model for Premium Risk is developed here for a multi-line Non-Life insurer. We follow a classical approach based on a Collective Risk Model properly extended in order to consider not only the volatility of aggregate claim amounts but also expense volatility. To measure the effect of risk mitigation, suitable reinsurance strategies are pursued. We analyze how naïve coverage as conventional Quota Share and Excess of Loss reinsurance may modify the exact moments of the distribution of technical results. Furthermore, we investigate how alternative choices of commission rates in proportional treaties may affect the variability of distribution. Numerical results are also figured out in the last part of the paper with evidence of different effects for small and large companies. The main reasons for these differences are pointed out.
- Published
- 2015
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3. Appunti di statistica II: Note a uso degli studenti
- Author
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Diego Zappa, Silvia Facchinetti, Silvia Facchinetti
- Published
- 2020
4. A comparison of geospatial models for car crash risk
- Author
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Andrea Bucci, Alfredo Cartone, Adelia Evangelista, Andrea Marletta, Cantaluppi, Gabriele, Giardino, Giorgio, Zappa, Diego, Gabriele Cantaluppi (ORCID:0000-0001-5234-3900), Diego Zappa (ORCID:0000-0003-4335-4530), Andrea Bucci, Alfredo Cartone, Adelia Evangelista, Andrea Marletta, Cantaluppi, Gabriele, Giardino, Giorgio, Zappa, Diego, Gabriele Cantaluppi (ORCID:0000-0001-5234-3900), and Diego Zappa (ORCID:0000-0003-4335-4530)
- Abstract
The assessment of risk related to car crashes in road networks is a topic relevant for both social impact as well as for the related political/administrative decisions. By means of Monte Carlo simulation we study the performance of different models aimed at evaluating the number of crash occurrences that can be expected at each road street segment level in a network of streets in a certain time window.
- Published
- 2023
5. Geo-referenced data and complex networks for measuring road accident risk
- Author
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Andrea Bucci, Alfredo Cartone, Adelia Evangelista, Andrea Marletta, Cantaluppi, Gabriele, Clemente, Gian Paolo, Della Corte, Francesco, Zappa, Diego, Gabriele Cantaluppi (ORCID:0000-0001-5234-3900), Gian Paolo Clemente (ORCID:0000-0001-6795-4595), Francesco Della Corte (ORCID:0000-0003-2436-8240), Diego Zappa (ORCID:0000-0003-4335-4530), Andrea Bucci, Alfredo Cartone, Adelia Evangelista, Andrea Marletta, Cantaluppi, Gabriele, Clemente, Gian Paolo, Della Corte, Francesco, Zappa, Diego, Gabriele Cantaluppi (ORCID:0000-0001-5234-3900), Gian Paolo Clemente (ORCID:0000-0001-6795-4595), Francesco Della Corte (ORCID:0000-0003-2436-8240), and Diego Zappa (ORCID:0000-0003-4335-4530)
- Abstract
The assessment of risk related to car crashes in road networks is a relevant topic for both social impact and the related political/administrative decisions. To this end, we show how the spatial objects and the information concerning the structure of the roads along with the crash history can be used to map the risk related to any road of a network. In particular, we follow a combined approach. On the one hand, a statistical model is developed in order to assess the risk on the basis of a set of features related to the characteristics of the streets. On the other hand, from the spatial object we build a weighted network, where the assessed risk of each segment is used as a weight. We study the topology structure of the graph and we show how classical network indicators can provide meaningful insights about the risk of an area.
- Published
- 2023
6. Model‐based process capability indices: The dry‐etching semiconductor case study
- Author
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Diego Zappa, Riccardo Borgoni, Borgoni, R, and Zappa, D
- Subjects
sampling network ,Materials science ,business.industry ,Process capability ,dry-etching semiconductor processes, mixture distributions, process capability indices, sampling network ,Management Science and Operations Research ,process capability indices ,Semiconductor ,Settore SECS-S/01 - STATISTICA ,Mixture Distributions ,Optoelectronics ,dry-etching semiconductor processes ,Dry etching ,Safety, Risk, Reliability and Quality ,business - Abstract
Process capability indices are widely used to check quality standards both at the production level and for business activity. They consider the location and the deviation from specification limits and targets. The literature contains many contributions on this topic both in the univariate and the multivariate context. Motivated by a real semiconductor case study, we discuss the role of rational subgroups and the challenge they present in the computation of capability indices, especially when data refer to lots of products. In addition, our context involves a mix of problems: unilateral specification limit, nonsymmetric distribution of the data, evidence of data from a mixture of distributions, and the need to filter one component of the mixture. After solving the previous issues and because of the peculiar characteristics of semiconductor processes based on the so called “wafers,” we contribute to the literature a proposal on how to compute capability indices in the case of heteroscedastic spatial processes. With a generalized additive model, we show that it is possible to estimate a capability surface that allows the identification of regions expected to not be fully compliant with the desired quality standards.
- Published
- 2020
7. Non‐parametric local capability indices for industrial planar manufacts: An application to the etching phase in the microelectronic industry
- Author
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Riccardo Borgoni, Vincenzo Emanuele Farace, Diego Zappa, Borgoni, R, Farace, V, and Zappa, D
- Subjects
Bayesian semiparametric quantile regression ,thin plate spline ,Settore SECS-S/01 - STATISTICA ,Modeling and Simulation ,microelectronics ,SECS-S/01 - STATISTICA ,dry etching ,Management Science and Operations Research ,General Business, Management and Accounting ,microelectronic - Abstract
Process capability indices are routinely used to estimate the mean-variability performance of industrial products with respect to both targets and specification limits. However, when the target variable is defined over a planar surface of a manufact, it is relevant to assess the capability of the production process locally, that is, at any spatial location of the surface, in particular if the manufact has to be split into pieces to obtain single production items. In this article, focusing on the specification introduced by Clements [Qual Prog., 22, 95–100], we suggest an approach based on additive quantile models to estimate, in a Bayesian paradigm, the index locally. We demonstrate its use in the context of the etching phase of the integrated circuit fabrication process. Since capability of etching processes is typically assessed for batches of wafers, we also propose two algorithms based on resampling to perform local capability analysis at the lot level.
- Published
- 2022
8. Appunti di statistica II: Note ad uso degli studenti
- Author
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Diego Zappa, Silvia Facchinetti
- Published
- 2013
9. Identification of spatial defects in semiconductor manufacturing
- Author
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Diego Zappa, Chiara Galimberti, Riccardo Borgoni, Borgoni, R, Galimberti, C, and Zappa, D
- Subjects
Computer science ,Semiconductor device fabrication ,business.industry ,Pattern recognition ,Management Science and Operations Research ,Minimum spanning tree ,p-value control chart ,General Business, Management and Accounting ,Identification (information) ,alpha-shapes ,Settore SECS-S/01 - STATISTICA ,Modeling and Simulation ,integrated circuits fabrication ,minimum spanning tree algorithm ,alpha-shape ,Artificial intelligence ,business ,p-value control charts ,Alpha shape - Abstract
In this article, we investigate the occurrence of defects in integrated circuit fabrication and show how spatial analysis can be effective in grasping and representing spatial regularities in defect patterns on the silicon supports, called wafers, used to produce microchips. Defects occurring on the wafer surface are the main cause of yield loss in the semiconductor industry; hence, to promptly detect an excess of defects and identify their spatial structure is crucial to the entire fabrication process. To address this hard-to-solve problem, this article proposes a concatenation of different methods, namely, a control chart, a clustering algorithm, and a graphical tool. First, a control chart based on the p-value of an appropriate test recognizes spatially structured defects on the wafer area. Then a clustering procedure grounded on the minimum spanning tree algorithm is adopted to identify those regions more prone to defect occurrences. Finally, alpha-shapes are employed to display their shape effectively. The suggested procedure proves to be extremely fast and effective, allowing its implementation in-line during the fabrication process. This provides a great advantage in modern microelectronics where items tend to be highly specialized and often produced in small lots. In particular, due to the Monte Carlo nature of the procedure, the control chart proposed hereafter does not require gold standard data to be set. This is particularly advantageous for small lot production, which is typically limited in time and does not permit to collect long time series of the charting statistics.
- Published
- 2021
10. Assessing the Risk of Car Crashes in Road Networks
- Author
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Andrea Gilardi, Riccardo Borgoni, Diego Zappa, Borgoni, R, Gilardi, A, and Zappa, D
- Subjects
Typology ,Index (economics) ,Sociology and Political Science ,05 social sciences ,Open data ,General Social Sciences ,050109 social psychology ,Regression analysis ,Crash ,Pedestrian ,Spatial data ,Transport engineering ,Arts and Humanities (miscellaneous) ,Settore SECS-S/01 - STATISTICA ,0502 economics and business ,Developmental and Educational Psychology ,Zero-inflated model ,0501 psychology and cognitive sciences ,Business ,050207 economics ,Spatial analysis ,Road safety index ,Zero inflated regression model - Abstract
Worldwide, thousands of people die annually in highway-related crashes and millions are injured. Hence, car wrecks have very high direct social costs but also relevant indirect economic effects such as an adverse impact on the burden of hospitalization and an increased health expenditure. The analysis of car crash data has long been used as a basis for influencing highway and vehicle designs but also, and perhaps more importantly, to support local authorities in allocating resources aimed at improving road safety and making political decisions to mitigate road risks in the most exposed areas. In this paper, we show how a range of information collected from open data sources concerning the structure of the road network (road typology, traffic lights, pedestrian crossings, etc.), socio-demographical dimensions and crash history can be proficiently used for this aim. We adopt a dynamic Zero Inflated Poisson (ZIP) regression model to define two indexes. The first index, derived from the counting component of the ZIP model, measures how prone to crash risk a segment is. The other, derived by the zero component of the ZIP model, represents a measure of the likelihood of segments to not be exposed to crashes. Focussing on the city of Milan (Northern Italy), we found that the most relevant determinant of road risk proneness is crash history and that structural characteristics of the road are much more relevant than demographic information. Finally, we show how this information can be spatialized to produce maps of crash proneness and predict future spatial risk indexes.
- Published
- 2021
11. Text Mining in Insurance: From Unstructured Data to Meaning
- Author
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Zappa, Diego, Borrelli, Mattia, Clemente, Gian Paolo, Savelli, Nino, Diego Zappa (ORCID:0000-0003-4335-4530), Gian Paolo Clemente (ORCID:0000-0001-6795-4595), Nino Savelli (ORCID:0000-0001-8818-8367), Zappa, Diego, Borrelli, Mattia, Clemente, Gian Paolo, Savelli, Nino, Diego Zappa (ORCID:0000-0003-4335-4530), Gian Paolo Clemente (ORCID:0000-0001-6795-4595), and Nino Savelli (ORCID:0000-0001-8818-8367)
- Abstract
Every day, insurance companies collect an enormous quantity of text data from multiple sources. We present a strategy to make beneficial use of the large amount of information available in documents by exploiting natural language processing. After a brief review of the basics of text mining, we describe a case study in which, by analyzing the accident narratives written by the researchers of the National Highway Traffic Safety Administration of the U.S. Department of Transportation, we aim to extract latent information that can be used to fine-tune policy premiums. The process involves two steps. First, we classify the reports according to the relevance of their content to determine the risk profiles of the people involved. Next, we use these profiles to create new latent risk covariates for a company’s ratemaking process.
- Published
- 2021
12. Assessing the risk of Car Crashes in Road Networks
- Author
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Borgoni Riccardo, Michelangeli Alessandra, Borgoni, Riccardo, Gilardi, Andrea, Zappa, Diego, Diego Zappa (ORCID:0000-0003-4335-4530), Borgoni Riccardo, Michelangeli Alessandra, Borgoni, Riccardo, Gilardi, Andrea, Zappa, Diego, and Diego Zappa (ORCID:0000-0003-4335-4530)
- Abstract
Worldwide thousands of people die annually in highway-related crashes and millions are injured. By 2030 highway-related crashes will be the 5-th leading cause of death in the world according to the WHO (Mannering and Bhat, 2014). The statistical analysis of accident data has historically been fundamental to develop road-safety policies aiming at saving lives and reducing injuries severity. In the absence of detailed driving-related data (such as acceleration and braking) and crash-related data (such as those made available from vehicle black-boxes) that would help improve the identification of cause and effect relationships, a large majority of research has addressed the problem in terms of understanding the factors that affect the frequency of crashes, i.e. the number of crashes occurring in some geographical space over some specified time periods.[...]
- Published
- 2021
13. Assessing the Risk of Car Accidents in Road Networks
- Author
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Andrea Gilardi, Riccardo Borgoni, Diego Zappa, Borgoni, R, Michelangeli, A, Gilardi, A, and Zappa, D
- Subjects
SECS-S/01 - STATISTICA ,Open data, spatial data, Zero inflated regression model - Published
- 2020
14. Spatial Modeling of Driver Crash Risk using Georeferenced Data
- Author
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Riccardo Borgoni, Gilardi Andrea, Diego Zappa, Borgoni, Riccardo, Michelangeli, Alessandra, Borgoni, R, Gilardi, A, and Diego, Z
- Subjects
Geospatial modeling ,Risk of accident ,Insurance Premium - Published
- 2020
15. Assessing the Risk of Car Accidents in Road Networks
- Author
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Borgoni, R, Michelangeli, A, Gilardi, A, Zappa, D, Andrea Gilardi, Riccardo Borgoni, Diego Zappa, Borgoni, R, Michelangeli, A, Gilardi, A, Zappa, D, Andrea Gilardi, Riccardo Borgoni, and Diego Zappa
- Published
- 2020
16. Spatial Modeling of Driver Crash Risk using Georeferenced Data
- Author
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Borgoni, Riccardo, Michelangeli, Alessandra, Borgoni, R, Gilardi, A, Diego, Z, Riccardo Borgoni, Gilardi Andrea, Diego Zappa, Borgoni, Riccardo, Michelangeli, Alessandra, Borgoni, R, Gilardi, A, Diego, Z, Riccardo Borgoni, Gilardi Andrea, and Diego Zappa
- Published
- 2020
17. Spatial modelling of driver crash risk using georeferenced data
- Author
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Borgoni Riccardo, Michelangeli Alessandra, Borgoni, Riccardo, Gilardi, Andrea, Zappa, Diego, Diego Zappa (ORCID:0000-0003-4335-4530), Borgoni Riccardo, Michelangeli Alessandra, Borgoni, Riccardo, Gilardi, Andrea, Zappa, Diego, and Diego Zappa (ORCID:0000-0003-4335-4530)
- Abstract
This contribution deals with a quite common but relevant issue in insurance. Suppose to be interested in covering a risk (e.g. motor accident, life/health risks, risk of disability, adverse weather events, etc...) and that you want to stipulate a contract to protect yourself against the loss of economic resources in case that the event occurs. In such circumstances any insurer will ask you to pay an amount (called premium) that approximately equals the pure premium (i.e. the amount that corresponds to the expected loss times the odds of the event) plus the so called loadings (i.e. the amount of money that consider both the gain of the contractor and administrative/process costs). The challenging point is: how to compute the odds? In particular, how to compute the probability of an adverse event given covariates that should be considered explanatory of the risk? [...]
- Published
- 2020
18. Modeling inter-country spatial financial interactions with Graphical Lasso: An application to sovereign co-risk evaluation
- Author
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Giuseppe Arbia, Riccardo Bramante, Silvia Facchinetti, and Diego Zappa
- Subjects
Economics and Econometrics ,Settore SECS-S/03 - STATISTICA ECONOMICA ,Conditional dependence ,Credit default swap ,Network dependence ,Computer science ,05 social sciences ,regional financial contagion ,Graph theory ,Context (language use) ,Spatial conditional dependence ,01 natural sciences ,Urban Studies ,010104 statistics & probability ,Lasso (statistics) ,Search algorithm ,0502 economics and business ,Systemic risk ,Econometrics ,050207 economics ,0101 mathematics ,Spurious relationship - Abstract
We propose a model to extract significant risk spatial interactions between countries adopting the Graphical Lasso algorithm, used in graph theory to sort out spurious conditional correlations. In this context, the major issue is the definition of the penalization parameter. We propose a search algorithm aimed at the best separation of the variables (expressed in terms of conditional dependence) given an a priori desired partition. The case study focuses on Credit Default Swap (CDS) returns over the period 2009–2017. The proposed algorithm is used to estimate the spatial systemic risk relationship between Peripheral and Core Countries in the Euro Area.
- Published
- 2018
19. Modelling Outstanding Claims with Mixed Compound Processes in insurance
- Author
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Nino Savelli, Gian Paolo Clemente, and Diego Zappa
- Subjects
Solvency II ,Solvency ,Collective risk model ,Actuarial science ,Computer science ,media_common.quotation_subject ,Reserve Risk ,General insurance ,Payment ,Settore SECS-S/06 - METODI MATEMATICI DELL'ECONOMIA E DELLE SCIENZE ATTUARIALI E FINANZIARIE ,Cox process ,Average cost method ,Line of business ,Capital requirement ,Probability distribution ,Stochastic models for claims reserve ,media_common - Abstract
In general insurance, measuring the uncertainty of future loss payments and estimating the claims reserve are primary goals of actuaries. To deal with these tricky tasks, a broad literature is available on deterministic and stochastic approaches, most of which aims at straightforwardly modelling the overall claims reserve. In this paper by an extended, very general and reproducible case-study, we analyze the reserving process by attributing to each cell of the lower part of the run-off triangle a Compound mixed Poisson Process, calibrated upon both the numbers of claims and future average costs and considering as well the dependence among incremental claims. We provide analytically the moments of both incremental payments and the total reserve. Furthermore, we accordingly consider the probability distribution of the claims reserve, which is necessary for the assessment of the Risk Reserve capital requirement in a Solvency II framework. To test the impact of the model under different scenarios, insurers and lines of business, the case study is thoroughly analyzed by exploiting the Fisher-Lange average cost method.
- Published
- 2019
20. Spatial Logistic Regression for Events Lying on a Network: Car Crashes in Milan
- Author
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Andrea Gilardi, Riccardo Borgoni, Diego Zappa, Giuseppe Arbia, Stefano Peluso, Alessia Pini and Giulia Rivellini, Gilardi, A, Borgoni, R, and Zappa, D
- Subjects
urban geography, car accidents, open data ,urban geography ,Settore SECS-S/01 - STATISTICA ,car accidents ,open data - Abstract
In this paper we propose a methodology to estimate the probability that a car accident occurs in urban roads. Our approach is based on logistic regression and takes into account the particular nature of the data which conforms to a spatial point pattern on a network. Using the open data on street networks provided within the OpenStreetMap project, we estimate the probability of car accidents for every street in the municipality of Milan.
- Published
- 2019
21. Online detection of financial time series peaks and troughs: A probability-based approach
- Author
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Silvia Facchinetti, Riccardo Bramante, and Diego Zappa
- Subjects
Settore SECS-S/03 - STATISTICA ECONOMICA ,Series (mathematics) ,Computer science ,time varying parameters ,Settore SECS-S/01 - STATISTICA ,Econometrics ,Turning point detection ,financial time series ,Analysis ,Computer Science Applications ,Information Systems - Published
- 2019
22. Monitoring Network Selection in Semiconductor Manufacturing Process
- Author
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Jens Bischoff, Agnes Backhausz, Rossella Berni, Sonja Kunt, Luis Macro-Almagro, Antonio Pievatolo, Marco Seavra dos ei, Murat Caner Testik, Borgoni, Riccardon, Zappa, Diego, Diego Zappa (ORCID:0000-0003-4335-4530), Jens Bischoff, Agnes Backhausz, Rossella Berni, Sonja Kunt, Luis Macro-Almagro, Antonio Pievatolo, Marco Seavra dos ei, Murat Caner Testik, Borgoni, Riccardon, Zappa, Diego, and Diego Zappa (ORCID:0000-0003-4335-4530)
- Abstract
The monitoring of spatial production processes typically involves sampling network to gather information about the status of the process. Sampling costs are often not marginal and once the process has been accurately calibrated, in order to save money, it might be appropriate to reduce the dimension of the sampling grid. In some cases, the allocation of a new network of less dimension is not free of constraints and it might be necessary the selection of a subgrid extracted from the original network (Borgoni and Zappa, 2017). We discuss a computer intensive method to find the best balance between the grid representativeness, i.e. how much the new grid represents the starting network, the grid accuracy, i.e. how much the new grid is close to the subgrid that will be extracted from the full network, and the spatial coverage, i.e. how much the selected subgrid is spatially spread and able to represent the sample space. In a not constrained context, the best allocation of a networks of a given size on a bounded domain is an issue widely discussed in the literature (see Dean et al, 2015, for a recent comprehensive book). In general, in our context, given a network defined according to some criteria, two are the issues we have to face with. First, how to find the subgrid maximally representative of both the sample space and the assigned grid. Furthermore, if no information are available about the shape of the response surface, because the experimental domain is circular, any grid can be rotated while keeping invariant its optimality property. Second, it is necessary to assess the prediction accuracy when the subgrid is used to estimate the expected response surface. Additionally, the procedure must be flexible so that it can be applied whatever is the initial network and able to include, if available, expert knowledge about sub regions. For example, in semiconductor processes, engineers know that the production process close to the borders of the wafer is often affected by
- Published
- 2019
23. Spatial Logistic Regression for Events Lying on a Network: Car Crashes in Milan
- Author
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Giuseppe Arbia, Stefano Peluso, Alessia Pini, Giulia Rivellini, Gilardi, Andrea, Borgoni, Riccardo, Zappa, Diego, Diego Zappa (ORCID:0000-0003-4335-4530), Giuseppe Arbia, Stefano Peluso, Alessia Pini, Giulia Rivellini, Gilardi, Andrea, Borgoni, Riccardo, Zappa, Diego, and Diego Zappa (ORCID:0000-0003-4335-4530)
- Abstract
In this paper we propose a methodology to estimate the probability that a car accident occurs in urban roads. Our approach is based on logistic regression and takes into account the particular nature of the data which conforms to a spatial point pattern on a network. Using the open data on street networks provided within the OpenStreetMap project, we estimate the probability of car accidents for every street in the municipality of Milan.
- Published
- 2019
24. Online detection of financial time series peaks and troughs: A probability-based approach
- Author
-
Bramante, Riccardo, Facchinetti, Silvia, Zappa, Diego, riccardo bramante (ORCID:0000-0002-2647-5720), silvia facchinetti (ORCID:0000-0002-3753-0406), diego zappa (ORCID:0000-0003-4335-4530), Bramante, Riccardo, Facchinetti, Silvia, Zappa, Diego, riccardo bramante (ORCID:0000-0002-2647-5720), silvia facchinetti (ORCID:0000-0002-3753-0406), and diego zappa (ORCID:0000-0003-4335-4530)
- Abstract
The problem related to the identification of a change in time series trajectories plays a crucial role in many contexts. In this paper, we propose a flexible and computationally efficient procedure for turning point identification based on hypothesis testing applied to the difference between two consecutive slopes in a rolling regression framework. Along with the description of the methodology, to measure the performance of the method we have applied it to the S&P 500 Stock Index and its subsector indices. By using an in-sample/out-of-sample approach we compare results with the profit/losses we could obtain by using themoving average crossover strategy. Results show that the operating signals obtained by our proposal may better enable financial analysts to make profitable decisions. Finally we present an extensive simulation study to show the weaknesses and strengths of the proposal under different expected returns and volatility scenarios.
- Published
- 2019
25. Control Limits
- Author
-
Diego ZAPPA
- Subjects
false alarm rate ,upper and lower control limits ,Settore SECS-S/01 - STATISTICA ,average run length ,control charts ,process control - Published
- 2016
26. Global versus local beta models: A partitioned distribution approach
- Author
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Diego Zappa and Riccardo Bramante
- Subjects
Economics and Econometrics ,050208 finance ,Truncated normal distribution ,Capital asset pricing model ,media_common.quotation_subject ,Mixture of distributions ,05 social sciences ,Minimum distance ,Disjoint sets ,portfolio optimization ,Normal distribution ,Minimum Distance Approach ,Settore SECS-S/01 - STATISTICA ,0502 economics and business ,Statistics ,Economics ,Econometrics ,050207 economics ,Portfolio optimization ,Finance ,Normality ,Stock (geology) ,media_common - Abstract
This study investigates the assumption that stock riskiness, captured by the market global beta, is constant over the market returns domain. To relax this assumption we propose to model stock returns through disjoint truncated normal distributions fit by means of the Minimum Distance Approach. This provides a set of disjoint conditional regions where normality still holds but it allows to decompose the unconditional beta into local ones referred to each region. In the case study we show that this approach, while preserving the simplicity of describing data by normal distributions, significantly improves the accuracy of the fit, compensating the well-known inadequacy of the standard normal distribution to fit returns in the tails. An extensive out of sample returns predictive test shows that the quality of prediction obtained with our methodology globally has similar statistical properties as for the standard global beta, but it outperforms the latter especially when the negative returns' domain is considered.
- Published
- 2016
27. The Impact of Reinsurance Strategies on Capital Requirements for Premium Risk in Insurance
- Author
-
Nino Savelli, Gian Paolo Clemente, and Diego Zappa
- Subjects
Reinsurance ,Solvency II ,Strategy and Management ,Economics, Econometrics and Finance (miscellaneous) ,Commission ,jel:C ,lcsh:HG8011-9999 ,lcsh:Insurance ,jel:M4 ,jel:K2 ,jel:G0 ,jel:G1 ,Accounting ,jel:G2 ,jel:G3 ,ddc:330 ,Capital requirement ,Risk management ,collective risk model ,Solvency ,Actuarial science ,Solvency ratio ,business.industry ,Settore SECS-S/06 - METODI MATEMATICI DELL'ECONOMIA E DELLE SCIENZE ATTUARIALI E FINANZIARIE ,jel:M2 ,capital requirement for premium risk ,reinsurance strategies ,Quota share ,Business ,Volatility (finance) - Abstract
New risk-based solvency requirements for insurance companies across European markets have been introduced by Solvency II and will come in force from 1 January 2016. These requirements, derived by a Standard Formula or an Internal Model, will be by far more risk-sensitive than the required solvency margin provided by the current legislation. In this regard, a Partial Internal Model for Premium Risk is developed here for a multi-line Non-Life insurer. We follow a classical approach based on a Collective Risk Model properly extended in order to consider not only the volatility of aggregate claim amounts but also expense volatility. To measure the effect of risk mitigation, suitable reinsurance strategies are pursued. We analyze how naïve coverage as conventional Quota Share and Excess of Loss reinsurance may modify the exact moments of the distribution of technical results. Furthermore, we investigate how alternative choices of commission rates in proportional treaties may affect the variability of distribution. Numerical results are also figured out in the last part of the paper with evidence of different effects for small and large companies. The main reasons for these differences are pointed out.
- Published
- 2015
28. Sovereign co-risk measures in the Euro Area
- Author
-
Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi, Davide Piacentino, Arbia, Giuseppe, Bramante, Riccardo, Facchinetti, Silvia, Zappa, Diego, Giuseppe Arbia (ORCID:0000-0001-5173-3931), Riccardo Bramante (ORCID:0000-0002-2647-5720), Silvia Facchinetti (ORCID:0000-0002-3753-0406), Diego Zappa (ORCID:0000-0003-4335-4530), Antonino Abbruzzo, Eugenio Brentari, Marcello Chiodi, Davide Piacentino, Arbia, Giuseppe, Bramante, Riccardo, Facchinetti, Silvia, Zappa, Diego, Giuseppe Arbia (ORCID:0000-0001-5173-3931), Riccardo Bramante (ORCID:0000-0002-2647-5720), Silvia Facchinetti (ORCID:0000-0002-3753-0406), and Diego Zappa (ORCID:0000-0003-4335-4530)
- Abstract
We propose a method to extract significant risk interactions between Countries adopting the Graphical Lasso algorithm, used in graph theory to sort out the spurious effect of common components. In this context, the major issue is the definition of the penalization parameter. We propose a search algorithm aimed at the best separation of the variables (expressed in terms of conditional dependence) given an a priori desired partition. The case study focuses on Sovereign Bond Yields over the period 2009–2017. The proposed algorithm is used in systemic risk estimation of the Euro area sovereigns.
- Published
- 2018
29. Selecting subgrids from a spatial monitoring network: Proposal and application in semiconductor manufactoring process
- Author
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Riccardo Borgoni, Diego Zappa, Borgoni, R, and Zappa, D
- Subjects
Computer science ,0211 other engineering and technologies ,monitoring networks ,02 engineering and technology ,Management Science and Operations Research ,computer.software_genre ,01 natural sciences ,Representativeness heuristic ,010104 statistics & probability ,dual response surface model ,dual response surface models ,Production (economics) ,statistics for microelectronics ,0101 mathematics ,Dimension (data warehouse) ,Safety, Risk, Reliability and Quality ,monitoring network ,Selection (genetic algorithm) ,021103 operations research ,dual response surface models, monitoring networks, statistics for microelectronics ,Process (computing) ,Sampling (statistics) ,statistics for microelectronic ,Grid ,Dual (category theory) ,Settore SECS-S/01 - STATISTICA ,Data mining ,computer - Abstract
The monitoring of spatial production processes typically involves sampling network to gather information about the status of the process. Sampling costs are often not marginal, and once the process has been accurately calibrated, it might be appropriate to reduce the dimension of the sampling grid. This aim is often achieved through the allocation of a brand new network of less dimension. In some cases that is not possible and it might be necessary the selection of a subgrid extracted from the original network. Motivated by a real semiconductor problem, we propose a method to extract a monitoring subgrid from a given one, based upon grid representativeness, accuracy, and spatial coverage of the subgrid and, if available, by expert knowledge of the weights to be assigned to those areas where production may need greater precision. Discussion is mainly focused on circular spatial domain, since, in microelectronics, the basic production support, called wafer, is a circle. Straightforward generalizations to different spatial domains are possible. Furthermore, conditionally upon the availability of experimental data, we check the loss of accuracy by fitting a dual mean-variance response surface on the reduced grid. Joining the latter information and the criteria used to select the subgrid, we provide additional guidelines on how to fine-tune the subgrid selection. Real case studies are used to show the effectiveness of the proposal.
- Published
- 2017
30. Statistics for microelectronics
- Author
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Riccardo Borgoni, Laura Deldossi, Diego Zappa, Luigi Radaelli, Borgoni, R, Deldossi, L, Radaelli, L, and Zappa, D
- Subjects
Settore SECS-S/01 - STATISTICA ,business.industry ,Computer science ,Modeling and Simulation ,SECS-S/01 - STATISTICA ,Microelectronics ,Management Science and Operations Research ,Statistics microelectronics ,business ,General Business, Management and Accounting ,Manufacturing engineering ,STATISTICS FOR MICROELECTRONICS - Abstract
This paper introduces a special issue entirely devoted to the topic 'Statistics for Microelectronics'. The relevance of the theme for this journal is due to both the primary role that microelectronics has in many business activities of the modern society and to the complexity of the production process of integrated circuits that are obtained by several different steps performed on a wafer (i.e., a thin silicon slice of a few inches of diameter). Advanced statistical methods are necessary to monitor and improve this process because the yield of this industrial manufacturing is typically tiny and requires a very high precision. After a brief review of the semiconductor manufacturing process, we point out how statistical methodologies can contribute to this. Finally, we introduce the papers that discuss some key aspects of this subject. Copyright (c) 2013 John Wiley & Sons, Ltd.
- Published
- 2013
31. Optimal reduction of a spatial monitoring grid: Proposals and applications in process control
- Author
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Valeria Tritto, Luigi Radaelli, Diego Zappa, Riccardo Borgoni, Borgoni, R, Radaelli, L, Tritto, V, and Zappa, D
- Subjects
Statistics and Probability ,In process control ,business.industry ,Computer science ,Applied Mathematics ,Kriging, Simulated annealing, Spatial sampling, Statistics for microelectronics ,Process (computing) ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Grid ,Simulated annealing ,Reduction (complexity) ,Kriging ,Computational Mathematics ,Simulating annealing ,Statistics for microelectronics ,Computational Theory and Mathematics ,Settore SECS-S/01 - STATISTICA ,Hardware_INTEGRATEDCIRCUITS ,Deposition (phase transition) ,Microelectronics ,Wafer ,Spatial sampling ,Process engineering ,business - Abstract
Deposition of silicon dioxide (SiO2) is a critical step of integrated circuit manufacturing; hence it is monitored during the manufacturing process at a grid of points defined on the wafer area. Since collecting thickness measurements is expensive, it is a compelling issue to investigate how a sub grid can be identified. A strategy based on spatial prediction and simulating annealing is proposed to tackle the problem which proved to be effective when applied to a real process. A diagnostic device for monitoring the deposition process is also discussed which can be usefully adopted in the day-to-day activity by practitioners acting in process control of a microelectronics fab. © 2012 Elsevier B.V. All rights reserved.
- Published
- 2013
32. Lower and Upper Specification Limits (LSL, USL)
- Author
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Diego Zappa
- Subjects
Control limits ,Settore SECS-S/01 - STATISTICA ,Process capability index ,Process control ,Control chart ,control charts ,Mathematics ,Reliability engineering ,capability index - Published
- 2016
33. Measurement uncertainty with nested mixed effect models
- Author
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Laura Deldossi and Diego Zappa
- Subjects
Measure (data warehouse) ,Engineering ,ANOVA gauge R&R ,business.industry ,Process (engineering) ,Quantitative Evaluations ,Management Science and Operations Research ,Statistics ,Mixed effects ,Measurement uncertainty ,Variance components ,Safety, Risk, Reliability and Quality ,business ,Focus (optics) - Abstract
Measurement uncertainty in experiments is receiving increasing interest among practitioners both for quantitative and qualitative evaluations. Applications may be found, for example, to compare experiments run in different laboratories, to certify the precision of gauges used in the masurement process or to measure uncertainty in a classification process, i.e. when objects are qualitatively evaluated by appraisers. In this paper, after having briefly presented how to measure uncertainty using gauge repeatability and reproducibility studies, we focus on a problem quite relevant for practitioners: how to deal with the presence of correlation among ‘replications’. This issue is common to many food/agriculture experiments. By a modification of a nested mixed effect design, we describe the impact of dependencies among replications on measurement capability. Copyright © 2011 John Wiley & Sons, Ltd.
- Published
- 2011
34. ISO 5725 and GUM: comparison and comments
- Author
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Diego Zappa and Laura Deldossi
- Subjects
Measure (data warehouse) ,Statistical design ,General Chemical Engineering ,Design of experiments ,Iso standards ,General Chemistry ,Physics::Data Analysis ,Statistics and Probability ,Industrial engineering ,GR&R ,ISO standard ,Settore SECS-S/01 - STATISTICA ,Statistics ,Safety, Risk, Reliability and Quality ,Instrumentation ,Mathematics ,Statistician - Abstract
We compare the approach to measure uncertainties proposed in ISO 5725 and GUM from a statistician point of view. In particular we give some warnings to the application of the expanded uncertainty introduced in GUM when the input variables are few and we report some considerations on the relevant role of the interactions among the input variables in the measurement equation as well as the role of statistical design of experiments to measure uncertainties.
- Published
- 2008
35. From tetraploid to diploid, a pangenomic approach to identify genes lost during synthetic diploidization of Eragrostis curvula
- Author
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Jose Carballo, Andrés Martin Bellido, Juan Pablo Selva, Diego Zappacosta, Cristian Andres Gallo, Emidio Albertini, Mario Caccamo, and Viviana Echenique
- Subjects
diploidization ,Eragrostis curvula ,plant reproduction ,ploidy ,apomixis ,Plant culture ,SB1-1110 - Abstract
IntroductionIn Eragrostis curvula, commonly known as weeping lovegrass, a synthetic diploidization event of the facultative apomictic tetraploid Tanganyika INTA cv. originated from the sexual diploid Victoria cv. Apomixis is an asexual reproduction by seeds in which the progeny is genetically identical to the maternal plant.MethodsTo assess the genomic changes related to ploidy and to the reproductive mode occurring during diploidization, a mapping approach was followed to obtain the first E. curvula pangenome assembly. In this way, gDNA of Tanganyika INTA was extracted and sequenced in 2x250 Illumina pair-end reads and mapped against the Victoria genome assembly. The unmapped reads were used for variant calling, while the mapped reads were assembled using Masurca software.ResultsThe length of the assembly was 28,982,419 bp distributed in 18,032 contigs, and the variable genes annotated in these contigs rendered 3,952 gene models. Functional annotation of the genes showed that the reproductive pathway was differentially enriched. PCR amplification in gDNA and cDNA of Tanganyika INTA and Victoria was conducted to validate the presence/absence variation in five genes related to reproduction and ploidy. The polyploid nature of the Tanganyika INTA genome was also evaluated through the variant calling analysis showing the single nucleotide polymorphism (SNP) coverage and allele frequency distribution with a segmental allotetraploid pairing behavior.DiscussionThe results presented here suggest that the genes were lost in Tanganyika INTA during the diploidization process that was conducted to suppress the apomictic pathway, affecting severely the fertility of Victoria cv.
- Published
- 2023
- Full Text
- View/download PDF
36. A novel approach to evaluate Repeatability and Reproducibility for ordinal data
- Author
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Laura Deldossi and Diego Zappa
- Subjects
Statistics and Probability ,Ordinal data ,Measurement systems analysis ,Reproducibility ,Cumulative distribution function ,Repeatability ,computer.software_genre ,CUB models ,Gauge capability ,Level of measurement ,Measurement system analysis ,Settore SECS-S/01 - STATISTICA ,Data mining ,computer ,Mathematics - Abstract
We propose a novel usage of CUB models in order to evaluate Repeatability and Reproducibility (R&R) for ordinal data in business and industrial experiments. This is a context where there is a small group of appraisers who have to evaluate a sample of objects classifying them according to ordinal categories. By comparing the cumulative distribution functions obtained fitting CUB models to judgments given by appraisers, we give both graphical and analytical instruments to assess R&R for an ordinal measurement system. The approach is applied to the real-life example reported in de Mast and van Wieringen (2010).
- Published
- 2014
37. A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula
- Author
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María Cielo Pasten, José Carballo, Jimena Gallardo, Diego Zappacosta, Juan Pablo Selva, Juan Manuel Rodrigo, Viviana Echenique, and Ingrid Garbus
- Subjects
Eragrostis curvula ,apomixis ,sRNA libraries ,miRNA ,miRNA-mRNA interaction ,Plant culture ,SB1-1110 - Abstract
Weeping lovegrass (Eragrostis curvula [Shrad.] Nees) is a perennial grass typically established in semi-arid regions, with good adaptability to dry conditions and sandy soils. This polymorphic complex includes both sexual and apomictic cytotypes, with different ploidy levels (2x-8x). Diploids are known to be sexual, while most polyploids are facultative apomicts, and full apomicts have also been reported. Plant breeding studies throughout the years have focused on achieving the introgression of apomixis into species of agricultural relevance, but, given the complexity of the trait, a deeper understanding of the molecular basis of regulatory mechanisms of apomixis is still required. Apomixis is thought to be associated with silencing or disruption of the sexual pathway, and studies have shown it is influenced by epigenetic mechanisms. In a previous study, we explored the role of miRNA-mRNA interactions using two contrasting E. curvula phenotypes. Here, the sexual OTA-S, the facultative Don Walter and the obligate apomictic Tanganyika cDNA and sRNA libraries were inquired, searching for miRNA discovery and miRNA expression regulation of genes related to the reproductive mode. This allowed for the characterization of seven miRNAs and the validation of their miRNA-target interactions. Interestingly, a kinesin gene was found to be repressed in the apomictic cultivar Tanganyika, targeted by a novel miRNA that was found to be overexpressed in this genotype, suggestive of an involvement in the reproductive mode expression. Our work provided additional evidence of the contribution of the epigenetic regulation of the apomictic pathway.
- Published
- 2022
- Full Text
- View/download PDF
38. On the use of the market model R-square as a measure of stock price efficiency
- Author
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Riccardo Bramante, Diego Zappa, and Giovanni Petrella
- Subjects
Delay ,Settore SECS-S/03 - STATISTICA ECONOMICA ,Coefficient of determination ,Financial economics ,General Business, Management and Accounting ,Price discovery ,Measure (mathematics) ,Stock price ,Direct measure ,Interpretation (model theory) ,Price efficiency ,Settore SECS-P/11 - ECONOMIA DEGLI INTERMEDIARI FINANZIARI ,Accounting ,Econometrics ,Economics ,R-square ,Market model ,Finance - Abstract
The R-square of the market model is a very popular measure of stock price efficiency. However, its interpretation is far from being unambiguous. Some scholars argue that the R-square is a direct measure of efficiency, others argue that the R-square is an indirect measure of efficiency. This paper contributes to the literature in two ways. First, we model the relationship between the market model R-square and the delay in the price discovery process and, second, we find that the correlation between R-square and delay is consistently negative. We conclude that the R-square is a direct measure of price efficiency.
- Published
- 2013
39. Confidence intervals for variance components in Measurement System Capability studies
- Author
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Laura Deldossi and Diego Zappa
- Subjects
Statistics and Probability ,Measurement systems analysis ,System of measurement ,Process (computing) ,Variance (accounting) ,Gauge (firearms) ,Confidence interval ,Robust confidence intervals ,Confidence coefficients ,repeatability and reproducibility ,modified large sample ,Settore SECS-S/01 - STATISTICA ,Statistics ,generalized confidence interval ,CDF-based nonparametric confidence interval ,Mathematics - Abstract
In Measurement System Analysis a relevant issue is how to find confidence intervals for the parameters used to evaluate the capability of a gauge. In literature approximate solutions are available but they produce so wide intervals that they are often not effective in the decision process. In this article we introduce a new approach and, with particular reference to the parameter γR, i.e., the ratio of the variance due to the process and the variance due to the instrument, we show that, under quite realistic assumptions, we obtain confidence intervals narrower than other methods. An application to a real microelectronic case study is reported.
- Published
- 2012
40. Quality Standards and Control Charts Applied to Customer Surveys
- Author
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Diego Zappa, Laura Deldossi, and Ron S. Kenett
- Subjects
Engineering ,Engineering management ,business.industry ,media_common.quotation_subject ,Control chart ,Quality (business) ,Operations management ,business ,media_common - Published
- 2011
41. Measurement Errors and Uncertainty: A Statistical Perspective
- Author
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Laura Deldossi and Diego Zappa
- Subjects
Ordinal data ,Measure (data warehouse) ,Observational error ,CUB ,Computer science ,System of measurement ,Perspective (graphical) ,measurment uncertainty ,Industrial engineering ,CUB models ,Settore SECS-S/01 - STATISTICA ,Bounded function ,GRR ,Categorical variable ,Categorical scale - Abstract
Evaluation of measurement systems is necessary in many industrial contexts. The literature on this topic is mainly focused on how to measure uncertainties for systems that yield continuous output. Few references are available for categorical data and they are briefly recalled in this paper. Finally a new proposal to measure uncertainty when the output is bounded ordinal is introduced.
- Published
- 2011
42. Calibration Confidence Regions Using Empirical Likelihood
- Author
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Diego Zappa
- Subjects
Computer science ,Calibration (statistics) ,Process (computing) ,empirical likelihood ,Function (mathematics) ,calibration ,Weighting ,Empirical likelihood ,Settore SECS-S/01 - STATISTICA ,Statistics ,Econometrics ,Point (geometry) ,Computational problem ,data depth ,Confidence region - Abstract
The literature on multivariate calibration shows an increasing interest in non-parametric or semiparametric methods. Using Empirical Likelihood (EL). we present a semiparametric approach to find multivariate calibration confidence regions and we show how a unique optimum calibration point may be found weighting the EL profile function. In addition, a freeware VBA for Excel© program has been implemented to solve the many relevant computational problems. An example taken from a process of a semiconductor industry is presented.
- Published
- 2007
43. Confidence Regions in Multivariate Calibration: A Proposal
- Author
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Diego Zappa and Silvia Salini
- Subjects
Distribution (mathematics) ,Observational error ,Calibration (statistics) ,Statistics ,Bayesian probability ,Process capability index ,Econometrics ,Context (language use) ,Mathematics ,Parametric statistics ,Confidence region - Abstract
Most of the papers on calibration are based on either classic or bayesian parametric context. In addition to the typical problems of the parametric approach (choice of the distribution for the measurement errors, choice of the model that links the sets of variables, etc.), a relevant problem in calibration is the construction of confidence region for the unknown levels of the explanatory variables. In this paper we propose a semiparametric approach, based on simplicial depth, to test the hypothesis of linearity of the link function and then how to find calibration depth confidence regions.
- Published
- 2005
44. Some Notes on Confidence Regions in Multivariate Calibration
- Author
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Silvia Salini and Diego Zappa
- Subjects
Statistics ,Multivariate calibration ,Depth function ,Humanities ,Mathematics - Abstract
La maggior parte dei lavori sulla taratura statistica univariata e multivariata propongono contributi in ambito parametrico inquadrato in un contesto statistico classico o bayesiano. Oltre alla presenza dei problemi tipici dell'approccio parametrico (scelta della legge distribuzionale, del modello, ecc.), uno dei problemi piu rilevanti e la costruzione di regioni di confidenza per gli incogniti livelli delle variabili esplicative. Al fine di superare alcuni di tali problemi, nel presente lavoro si propone un approccio di tipo semiparametrico, basato sulla nozione di funzione depth: l'obiettivo e, dopo aver verificato con un apposito test se il modello di taratura puo essere di tipo lineare, costruire regioni depth di taratura nell'intorno degli incogniti livelli delle vere misure dell'esperimento.
- Published
- 2003
45. ‘Statistics for microelectronics’, ASMBI, v. 29, issue 4, July/August 2013, pages 315-318
- Author
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Diego Zappa, Luigi Radaelli, Laura Deldossi, and Riccardo Borgoni
- Subjects
Engineering ,business.industry ,Modeling and Simulation ,Library science ,Microelectronics ,Management Science and Operations Research ,business ,General Business, Management and Accounting - Published
- 2013
46. The role of statistical methodogies in the study or quality design of apparatuses and production systems
- Author
-
Umberto Magagnoli and Diego Zappa
- Subjects
Engineering ,Total quality management ,business.industry ,Industrial production ,media_common.quotation_subject ,Random effects model ,Industrial engineering ,Field (computer science) ,Industrial design ,Production (economics) ,Control chart ,Quality (business) ,business ,Simulation ,media_common - Abstract
Up to the 60s the design criteria of industrial production systems were mainly based on the new findings achieved in that period in technical and physical environments and generally on techniques having deterministic nature. Examples can be typically found in the field of structural project that must support mechanic, dielectric and thermic stress. The functional forms of the relationship between stress (cause), strain and performance changes (effects) were supposed to be known using variables or parameters related to the size of the item under consideration. This kind of design enabled the collection and control of the unknown and random components by using the so-called safety coefficients. As a consequence of this approach, the existence of random effects in industrial work was ignored and a deterministic point of view was accepted.
- Published
- 1995
47. Eragrostis curvula, a Model Species for Diplosporous Apomixis
- Author
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Jose Carballo, Diego Zappacosta, Juan Pablo Selva, Mario Caccamo, and Viviana Echenique
- Subjects
cytoembryology ,ovule development ,reproduction ,Botany ,QK1-989 - Abstract
Eragrostis curvula (Schrad.) Ness is a grass with a particular apomictic embryo sac development called Eragrostis type. Apomixis is a type of asexual reproduction that produces seeds without fertilization in which the resulting progeny is genetically identical to the mother plant and with the potential to fix the hybrid vigour from more than one generation, among other advantages. The absence of meiosis and the occurrence of only two rounds of mitosis instead of three during embryo sac development make this model unique and suitable to be transferred to economically important crops. Throughout this review, we highlight the advances in the knowledge of apomixis in E. curvula using different techniques such as cytoembryology, DNA methylation analyses, small-RNA-seq, RNA-seq, genome assembly, and genotyping by sequencing. The main bulk of evidence points out that apomixis is inherited as a single Mendelian factor, and it is regulated by genetic and epigenetic mechanisms controlled by a complex network. With all this information, we propose a model of the mechanisms involved in diplosporous apomixis in this grass. All the genetic and epigenetic resources generated in E. curvula to study the reproductive mode changed its status from an orphan to a well-characterised species.
- Published
- 2021
- Full Text
- View/download PDF
48. A High-Density Linkage Map of the Forage Grass Eragrostis curvula and Localization of the Diplospory Locus
- Author
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Diego Zappacosta, Jimena Gallardo, José Carballo, Mauro Meier, Juan Manuel Rodrigo, Cristian A. Gallo, Juan Pablo Selva, Juliana Stein, Juan Pablo A. Ortiz, Emidio Albertini, and Viviana Echenique
- Subjects
linkage map ,weeping lovegrass ,apomixis ,polyploid ,QTL ,synteny ,Plant culture ,SB1-1110 - Abstract
Eragrostis curvula (Schrad.) Nees (weeping lovegrass) is an apomictic species native to Southern Africa that is used as forage grass in semiarid regions of Argentina. Apomixis is a mechanism for clonal propagation through seeds that involves the avoidance of meiosis to generate an unreduced embryo sac (apomeiosis), parthenogenesis, and viable endosperm formation in a fertilization-dependent or -independent manner. Here, we constructed the first saturated linkage map of tetraploid E. curvula using both traditional (AFLP and SSR) and high-throughput molecular markers (GBS-SNP) and identified the locus controlling diplospory. We also identified putative regulatory regions affecting the expressivity of this trait and syntenic relationships with genomes of other grass species. We obtained a tetraploid mapping population from a cross between a full sexual genotype (OTA-S) with a facultative apomictic individual of cv. Don Walter. Phenotypic characterization of F1 hybrids by cytoembryological analysis yielded a 1:1 ratio of apomictic vs. sexual plants (34:27, X2 = 0.37), which agrees with the model of inheritance of a single dominant genetic factor. The final number of markers was 1,114 for OTA-S and 2,019 for Don Walter. These markers were distributed into 40 linkage groups per parental genotype, which is consistent with the number of E. curvula chromosomes (containing 2 to 123 markers per linkage group). The total length of the OTA-S map was 1,335 cM, with an average marker density of 1.22 cM per marker. The Don Walter map was 1,976.2 cM, with an average marker density of 0.98 cM/marker. The locus responsible for diplospory was mapped on Don Walter linkage group 3, with other 65 markers. QTL analyses of the expressivity of diplospory in the F1 hybrids revealed the presence of two main QTLs, located 3.27 and 15 cM from the diplospory locus. Both QTLs explained 28.6% of phenotypic variation. Syntenic analysis allowed us to establish the groups of homologs/homeologs for each linkage map. The genetic linkage map reported in this study, the first such map for E. curvula, is the most saturated map for the genus Eragrostis and one of the most saturated maps for a polyploid forage grass species.
- Published
- 2019
- Full Text
- View/download PDF
49. Differential Methylation Patterns in Apomictic vs. Sexual Genotypes of the Diplosporous Grass Eragrostis curvula
- Author
-
Jose Carballo, Diego Zappacosta, Gianpiero Marconi, Jimena Gallardo, Marco Di Marsico, Cristian A. Gallo, Mario Caccamo, Emidio Albertini, and Viviana Echenique
- Subjects
apomixis ,DNA methylation ,diplospory ,eragrostis ,epigenetics ,Botany ,QK1-989 - Abstract
DNA methylation is an epigenetic mechanism by which a methyl group is added to a cytosine or an adenine. When located in a gene/regulatory sequence it may repress or de-repress genes, depending on the context and species. Eragrostis curvula is an apomictic grass in which facultative genotypes increases the frequency of sexual pistils triggered by epigenetic mechanisms. The aim of the present study was to look for correlations between the reproductive mode and specific methylated genes or genomic regions. To do so, plants with contrasting reproductive modes were investigated through MCSeEd (Methylation Context Sensitive Enzyme ddRad) showing higher levels of DNA methylation in apomictic genotypes. Moreover, an increased proportion of differentially methylated positions over the regulatory regions were observed, suggesting its possible role in regulation of gene expression. Interestingly, the methylation pathway was also found to be self-regulated since two of the main genes (ROS1 and ROS4), involved in de-methylation, were found differentially methylated between genotypes with different reproductive behavior. Moreover, this work allowed us to detect several genes regulated by methylation that were previously found as differentially expressed in the comparisons between apomictic and sexual genotypes, linking DNA methylation to differences in reproductive mode.
- Published
- 2021
- Full Text
- View/download PDF
50. An in vitro assay for pre-screening resistance to Fusarium head blight in durum wheat
- Author
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Daniela SORESI, Diego ZAPPACOSTA, Antonio GARAYALDE, Rubén MIRANDA, and Alicia CARRERA
- Subjects
Fusarium graminearum ,FHB ,scab ,Botany ,QK1-989 - Abstract
Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a wheat disease that causes yield losses and mycotoxin accumulation in the grain. In germplasm of durum wheat (Triticum turgidum L. ssp. durum), genetic variability for resistance is scarce. To develop an improved assay for FHB resistance in wheat, we evaluated the effect of F. graminearum inoculation on seed germination and seedling growth and relationships of these parameters with disease severity. A genotype set was examined, comprising ten durum wheats as well as two common wheats, including susceptible and introgressed resistant lines. Genotypes were additionally screened using molecular markers linked to known FHB resistance QTLs. Significant differences were detected among inoculated genotypes for germination, coleoptile length, coleoptile weight, root weight and disease severity. Seedling variables were shown to be correlated across experiments validating repeatability of the in vitro assay. Using multiple regression analysis, coleoptile length was correlated with FHB severity (P
- Published
- 2015
- Full Text
- View/download PDF
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