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Practically, high-yield conversion of biomass into value-added products at low cost is a primary goal for any lignocellulosic refinery. In the industrial context, the limitation in the practical adaptation of the conventional techniques practically involves multiple reactors for the conversion of biomass to bioproducts. Therefore, the present manuscript critically reviewed the advancements in one-pot reaction systems with a major focus on the scientific production of value-added products from lignocellulosic biomass. In view of that, the novelty of one-pot reactions is shown during the fractionation of biomass into their individual constituents. The importance of the direct conversion of cellulose and lignin into a range of valuable products including organic acids and platform chemicals are separately discussed. Finally, the article is concluded with the opportunities, existing troubles, and possible solutions to overcome the challenges in lignocellulosic biorefinery. This article will assist the readers to identify the economic-friendly-one-pot conversion of lignocellulosic biomass.

The application of on-site produced cellulolytic enzymes in place of commercial enzymes towards hydrolytic preparations of reducing sugars using inexpensive lignocellulosic wastes is considered the most efficient strategy to accomplish a cost-effective biofuel production process. Along with improved production, intrinsic and systematic performance evaluation of the produced enzyme during the hydrolysis process through kinetic intervention remains a crucial requirement for achieving the improved performance of the process. With this motivation, the present study primarily deals with the nutritionally optimized production strategy of cellulases from rice straw (RS) waste using Trichoderma reesei (MTCC 164). The highest cellulase production was obtained 8.09 ± 0.32 g/l in batch mode at optimized combinations of 3.5% (w/v) RS inducer, 3.0% (w/v) lactose, and 1.5% (w/v) peptone. Production was further improved through pH-regulated (pH 5.5 to 6.5) fed-batch fermentations. The enzyme produced at pH 6 was considered for hydrolysis studies at 4 to 10% (w/w) solid loading due to reasonable exoglucanase, endoglucanase, and maximum β-glucosidase activity levels of 9.3 U/ml, 3.87 U/ml, and 2.65 U/ml respectively. Multi-reaction systematic kinetic modeling was implemented to evaluate enzyme performance during hydrolysis, and the values of inhibitory kinetic parameters (K

Present study focused on optimizing bioprocess condition for microalgal lutein production. From previous baseline yields of biomass (3.46 g/L) and lutein (13.7 mg/g), this study examined few key parameters. The 3X:3X ratio macro- and micronutrients was the most affecting parameter with highest biomass and lutein yields of 4.61 g/L and 14.3 mg/g. Temperature 30 °C enhanced the lutein up to 17.3 mg/g but reduced the biomass to 3 g/L. The light effects study showed 10k lux was most effective for lutein up to 14 mg/g, and effect of increasing salinity (25-75%) was detrimental. All the above parameters' optimization resulted in a lipid content of 22.5-26.5%. A maximum lutein productivity and yield of 0.451 mg/L/d and 65.74 mg/L with a 3X:3X macro- and micronutrient ratio was achieved. The Chlorella sorokiniana Kh12 strain exhibited one of the highest yields among recent reports; hence it could be a source for commercial lutein production.

Reducing sugar generation from lignocellulosic biomass (LCB) is closely linked with biomass characteristics, pretreatment and enzymatic hydrolysis conditions. In this study curated experimental data from literature was used to develop multivariate regression and artificial neural network (ANN) model considering nine predictors (i.e., cellulose, hemicellulose, lignin content, cellulose-lignin ratio, acid concentration, temperature, time, pretreatment severity, and enzyme concentration). Selected reduced polynomial model (R

In the continual march to a predominantly urbanized civilization, anthropogenic activities have increased scrupulously, industrialization have occurred, economic growth has increased, and natural resources are being exploited, causing huge waste management problems, disposal issues, and the evolution of several pollutants. In order to have a sustainable environment, these pollutants need to be removed and degraded. Bioremediation employing microorganisms or enzymes can be used to treat the pollutants by degrading and/or transforming the pollutants into different form which is less or non-toxic to the environment. Laccase is a diverse enzyme/biocatalyst belonging to the oxidoreductase group of enzymes produced by microorganisms. Due to its low substrate specificity and monoelectronic oxidation of substrates in a wide range of complexes, it is most commonly used to degrade chemical pollutants. For degradation of emerging pollutants, laccase can be efficiently employed; however, large-scale application needs reusability, thermostability, and operational stability which necessitated strategies like immobilization and engineering of robust laccase possessing desirable properties. Immobilization of laccase for bioremediation, and treatment of wastewater for degrading emerging pollutants have been focussed for sustainable development. Challenges of employing biocatalysts for these applications as well as engineering robust laccase have been highlighted in this study.

The novel therapeutic agents are frequently produced from marine actinomycetes and are still being explored due to their vast diversity. In this research, we isolated five marine actinomycetes (BN01, BN12, BN13, BN14 and GB15) from the soil sediments of the coastal region of Chennai, Tamil Nadu, India. The morphological and physicochemical properties of the isolates were characterized. The 16S rRNA sequencing analysis showed that the isolates were belonging to two genera namely Streptomyces and Saccharopolyspora. The extracellular crude secondary metabolites were extracted from the culture filtrate of all the marine actinomycetes isolated and tested for the antimicrobial, antioxidant and anticancer potential adopting standard methods. The results revealed that the extracts of the isolates BN01, BN14, and GB15 exhibited antimicrobial activity against M. luteus, E. coli, and S. aureus, with the maximum zone of inhibition of 19 mm, 21 mm, and 27 mm respectively, at the concentration of 10 mg/mL. The extract of the isolate BN12 showed excellent radical scavenging activity of 97.75 % at the concentration of 60 µg/mL and has the half-maximal inhibitory concentration (IC50) of 8.66 µg/mL. The anticancer activity results showed that the extract of the isolate BN12 had better inhibitory activity against the MCF 7 cell line with the IC50 value of 261.01 µg/µL.

Consolidated bioprocessing (CBP) is characterized by a single-step production of value-added compounds directly from biomass in a single vessel. This strategy has the capacity to revolutionize the whole biorefinery concept as it can significantly reduce the infrastructure input and use of chemicals for various processing steps which can make it economically and environmentally benign. Although the proof of concept has been firmly established in the past, commercialization has been limited due to the low conversion efficiency of the technology. Either a native single microbe, genetically modified microbe or a consortium can be employed. The major challenge in developing a cost-effective and feasible CBP process is the recognition of bifunctional catalysts combining the capability to use the substrates and transform them into value-added products with high efficiency. This article presents an in-depth analysis of the current developments in CBP around the globe and the possibilities of advancements in the future.

A systematic evaluation of microorganism's potential towards biosynthesis of cellulases from inexpensive lignocellulosic feedstock through appropriate kinetic modelling facilitates understanding, optimization and designing of an effective industrial cellulase enzyme production process. The present study aims to optimize a submerged fungal cultivation strategy for cellulase production from abundantly available newspaper wastes (NPW). A combined pretreatment strategy consisting diluted, 1% (v v

Pennisetum purpureum, commonly known as elephant grass, possesses an immense ability to be recognized as bioenergy crop due to its high carbohydrate content and ample availability in the northeastern states of India. In the present study, an alkaline treatment was conducted using NaOH of different concentrations (1%, 2% and 3% (w/v)) at varying reaction periods of 30, 60 and 90 min with an aspiration of maximum delignification and an enhanced recovery of carbohydrates from biomass. A maximum delignification of 81.0% (w/w) was obtained when the biomass was pretreated with 2% (w/v) of NaOH for 60 min while in the presence of 3% (w/v) of NaOH, the highest cellulose recovery of 72.3% (w/w) was accounted at 90 min of reaction time. During saccharification of biomass, a maximum 18.4 g/L of glucose was produced using 20 filter paper unit of commercial cellulase after 48 h of enzymatic reaction with a cellulose conversion of more than 46%. Furthermore, product inhibition study showed the significant inhibitory effect on enzymatic production of sugars even in the presence of 2 g/L glucose and xylose in the reaction system. The severity of alkaline treatment of the untreated and treated biomass was evaluated using different physiochemical analyses. Moreover, the extracted lignin obtained after acid precipitation of alkaline filtrate was characterised using UV-Visible spectrophotometer. Finally, two-way ANOVA revealed a significant variation in the production of glucose and xylose from biomass even when glucose was added at a fixed concentration (P value = 0.00044 <

In the present context of energy crisis, exploration of lignocellulosic biomass has emerged as potential substitute to maintain environmental sustainability. However, the conversion of biomass into value-added products still faces challenges to find a suitable unit operation. The stubborn dependency on the cost intensive enzymatic system, limits an effective saccharification to hydrolyze the biomass. India has been one of the top most agriculturally enriched countries with broad scope of utilizing waste residue that remains as an unused biomass at harvested locations. Hence, in the present script, an overview on the latest R&D initiatives taken by Government of India are briefed to highlight the projects based on biofuels and in addition, global scenario of biofuel production is comprehensively discussed. Further, critical analysis on the advancement of different pretreatment operations are highlighted through latest inventions. Thereafter, biochemistry of cellulase enzyme with essential factors were explored to understand the mechanistic interactions involved during saccharification of biomass. An insight on the latest accomplishments of various fermentation process provides an in depth understanding of metabolic engineering based on the genetic studies of fermentative microorganisms. Finally, the article is concluded with brief discussions on fate of the derivatives obtained from macromolecules such as cellulose and lignin.

Over the last few decades, the globe has much relied on fossil fuels; however, environmental concerns forced the World to look at biofuel as an alternative for stable economic development. Biofuel also facilitates national energy security maintenance and reduces environmental complications. The present study is focused on an in-depth analysis of bioenergy policy measures undertaken by various federal agencies of different countries in order to shed light on the bottlenecks that impede biofuel's growth as a sustainable and alternative fuel. An in-depth assessment of feedstock utilization, blending targets, and policy assistance schemes have been thoroughly reviewed. In addition, the potential of commercial firms for the production of bioenergy is highlighted in order to grasp the current bioenergy market scenario better. Finally, the article is concluded with the viewpoints of the authors to address the standing issues of global bioenergy generation.

Rapidly changing bioremediation prospects are key drive to develop sustainable options that can offer extra benefits rather than only environmental remediation. Algal remediating is gaining utmost attention due to its mesmerising sustainable features, removing odour and toxicity, co-remediating numerous common and emerging inorganic and organic pollutants from gaseous and aqueous environments, and yielding biomass for a range of valuable products refining. Moreover, it also improves carbon footprint via carbon-capturing offers a better option than any other non-algal process for several high CO

Bacterial cellulose (BC) represents a novel bio-origin nonomaterial with its unique properties having diverse applications. Increased market demand and low yield are the major reason for its higher cost. Bacteria belonging to Komagataeibacter sp are the most exploited ones for BC production. Development of a cost-effective bioprocess for higher BC production is desirable. Though static fermentation modes have been majorly employed for BC production using tray fermenters, agitated mode has also been employed successfully with air-lift fermenters as well as stirred tank reactors. Bioprocess advances in recent years has led BC production to an upper level; however, challenges of aeration requirement and labor cost towards the higher end is associated with static cultivation at large scale. We have discussed the bioprocess development for BC production in recent years along with the challenges associated and the path forward.

There is an immediate global requirement for an ingenious strategy for food waste conversion to biofuels in order to replace fossil fuels with renewable resources. Food waste conversion to bioethanol could lead to a sustainable process having the dual advantage of resolving the issue of food waste disposal as well as meeting the energy requirements of the increasing population. Food waste is increasing at the rate of 1.3 billion tonnes per year, considered to be one-third of global food production. According to LCA studies discarding these wastes is detritus to the environment, therefore; it is beneficial to convert the food waste into bioethanol. The CO

Increased urbanization and industrialization accelerated demand for energy, large-scale waste output, and negative environmental consequences. Therefore, the implementation of an effective solid-waste-management (SWM) policy for the handling of food waste is of great importance. The global food waste generation is estimated at about 1.6 gigatons/yr which attributes to an economic revenue of 750 billion USD. It can be converted into high-value enzymes, surfactants, Poly-hydroxybutyrate, biofuels, etc. However, the heterogeneous composition of food with high organic load and varying moisture content makes their transformation into value-added products difficult. This review aims to bring forth the possibilities and repercussions of food waste management. The socio-economic challenges related to SWM are comprehensively discussed particularly in terms of environmental concern. The engineering aspect in the collection, storage, and biotransformation of food waste into useful value-added products such as biofuels, advanced biomaterials, bioactive compounds, and platform chemicals are critically reviewed for efficient food waste management.

The rising consumption of the popular non-alcoholic beverage tea and its derivative products caused massive growth in worldwide tea production in the last decade, leading to the generation of huge quantities of waste tea residues every year. Most of these wastes are usually burnt or disposed in landfills without proper treatment which results in serious environmental issues by polluting water, air and soil. In the recent times, 'waste to wealth' is a fast-growing concept for environment friendly sustainable development. Utilization of the large amount of tea wastes for the production of low-cost adsorbents to reduce the expenses of water and wastewater treatment can be a sustainable way of management of these wastes which at the same time will improve circular economy also. This review endeavours to evaluate the potential of both raw and modified tea wastes towards the adsorption of pollutants from wastewater. The production of various adsorptive materials such as biochar, activated carbon, nanocomposites, hydrogels, nanoparticles from tea wastes are summarized. The advancements in their applications for the removal of different emerging contaminants from wastewater as well as potable water, air and soil are exhaustively reviewed. The outcome of the present review reveals that tea waste and its derivatives are appropriate candidates to be used as adsorbents that show tremendous effectiveness in cleaning the environment. This article will provide the readers with an in-depth knowledge on the sustainable utilization of tea waste as adsorbent materials and will assist them to explore this abundant cheap waste biomass for environmental remediation.