432 results on '"Diaz-Perales, A."'
Search Results
2. Virus-like Particles as Vaccines for Allergen-Specific Therapy: An Overview of Current Developments
- Author
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Helena Berreiros-Hortala, Gonzalo Vilchez-Pinto, Araceli Diaz-Perales, Maria Garrido-Arandia, and Jaime Tome-Amat
- Subjects
virus-like particles ,therapy ,allergy ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Immune engineering and modulation are the basis of a novel but powerful tool to treat immune diseases using virus-like particles (VLPs). VLPs are formed by the viral capsid without genetic material making them non-infective. However, they offer a wide variety of possibilities as antigen-presenting platforms, resulting in high immunogenicity and high efficacy in immune modulation, with low allergenicity. Both animal and plant viruses are being studied for use in the treatment of food allergies. These formulations are combined with adjuvants, T-stimulatory epitopes, TLR ligands, and other immune modulators to modulate or enhance the immune response toward the presented allergen. Here, the authors present an overview of VLP production systems, their immune modulation capabilities, and the applicability of actual VLP-based formulations targeting allergic diseases.
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- 2024
- Full Text
- View/download PDF
3. Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTipTM Probes: Application to the Pru p 3-Phytosphingosine System
- Author
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Rafael Daza, María Garrido-Arandia, Daniel Corregidor-Ortiz, Carla Isabel Pérez, Luis Colchero, Raquel Tabraue-Rubio, Manuel Elices, Gustavo V. Guinea, Araceli Diaz-Perales, and José Pérez-Rigueiro
- Subjects
affinity atomic force microscopy ,force spectroscopy ,AFM ,functionalization ,phytosphingosine ,lipid transfer protein ,Technology - Abstract
The interaction between the plant lipid transfer protein Pru p 3 and phytosphingosine was assessed using an atomic force microscope. Phytosphingosine was covalently immobilized on DeepTipTM probes and Pru p 3 on MicroDeckTM functionalized substrates. Single-molecular interaction events between both molecules were retrieved and classified and the distribution for each one of the identified types was calculated. A success rate of over 70% was found by comparing the number of specific Pru p 3-phytosphingosine interaction events with the total number of recorded curves. The analysis of the distribution established among the various types of curves was further pursued to distinguish between those curves that can mainly be used for assessing the recognition between phytosphingosine (sensor molecule) and Pru p 3 (target molecule) in the context of affinity atomic force microscopy, and those that entail details of the interaction and might be employed in the context of force spectroscopy. The successful application of these functionalized probes and substrates to the characterization of the low-intensity hydrophobic interaction characteristic of this system is a clear indication of the potential of exploiting this approach with an extremely wide range of different biological molecules of interest. The possibility of characterizing molecular assembly events with single-molecule resolution offers an advantageous procedure to plough into the field of molecular biomimetics.
- Published
- 2023
- Full Text
- View/download PDF
4. Are Physicochemical Properties Shaping the Allergenic Potency of Plant Allergens?
- Author
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Costa, Joana, Bavaro, Simona Lucia, Benedé, Sara, Diaz-Perales, Araceli, Bueno-Diaz, Cristina, Gelencser, Eva, Klueber, Julia, Larré, Colette, Lozano-Ojalvo, Daniel, Lupi, Roberta, Mafra, Isabel, Mazzucchelli, Gabriel, Molina, Elena, Monaci, Linda, Martín-Pedraza, Laura, Piras, Cristian, Rodrigues, Pedro M., Roncada, Paola, Schrama, Denise, Cirkovic-Velickovic, Tanja, Verhoeckx, Kitty, Villa, Caterina, Kuehn, Annette, Hoffmann-Sommergruber, Karin, and Holzhauser, Thomas
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- 2022
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5. Are Physicochemical Properties Shaping the Allergenic Potency of Animal Allergens?
- Author
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Costa, Joana, Villa, Caterina, Verhoeckx, Kitty, Cirkovic-Velickovic, Tanja, Schrama, Denise, Roncada, Paola, Rodrigues, Pedro M., Piras, Cristian, Martín-Pedraza, Laura, Monaci, Linda, Molina, Elena, Mazzucchelli, Gabriel, Mafra, Isabel, Lupi, Roberta, Lozano-Ojalvo, Daniel, Larré, Colette, Klueber, Julia, Gelencser, Eva, Bueno-Diaz, Cristina, Diaz-Perales, Araceli, Benedé, Sara, Bavaro, Simona Lucia, Kuehn, Annette, Hoffmann-Sommergruber, Karin, and Holzhauser, Thomas
- Published
- 2022
- Full Text
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6. NLRP3 priming due to skin damage precedes LTP allergic sensitization in a mouse model
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Pazos-Castro, Diego, Gonzalez-Klein, Zulema, Montalvo, Alma Yuste, Hernandez-Ramirez, Guadalupe, Romero-Sahagun, Alejandro, Esteban, Vanesa, Garrido-Arandia, Maria, Tome-Amat, Jaime, and Diaz-Perales, Araceli
- Published
- 2022
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- View/download PDF
7. NLRP3 priming due to skin damage precedes LTP allergic sensitization in a mouse model
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Diego Pazos-Castro, Zulema Gonzalez-Klein, Alma Yuste Montalvo, Guadalupe Hernandez-Ramirez, Alejandro Romero-Sahagun, Vanesa Esteban, Maria Garrido-Arandia, Jaime Tome-Amat, and Araceli Diaz-Perales
- Subjects
Medicine ,Science - Abstract
Abstract Allergic sensitization is initiated by protein and epithelia interaction, although the molecular mechanisms leading this encounter toward an allergic phenotype remain unknown. Here, we apply the two-hit hypothesis of inflammatory diseases to the study of food allergy sensitization. First, we studied the effects of long-term depilation in mice by analyzing samples at different time points. Several weeks of depilation were needed until clear immunological changes were evidenced, starting with upregulation of NLRP3 protein levels, which was followed by overexpression of Il1b and Il18 transcripts. Secondly, we assessed the effects of allergen addition (in this case, Pru p 3 in complex with its natural lipid ligand) over depilated skin. Systemic sensitization was evaluated by intraperitoneal provocation with Pru p 3 and measure of body temperature. Anaphylaxis was achieved, but only in mice sensitized with Prup3_complex and not treated with the NLRP3 inhibitor MCC950, thus demonstrating the importance of both hits (depilation + allergen addition) in the consecution of the allergic phenotype. In addition, allergen encounter (but not depilation) promoted skin remodeling, as well as CD45+ infiltration not only in the sensitized area (the skin), but across several mucosal tissues (skin, lungs, and gut), furtherly validating the systemization of the response. Finally, a low-scale study with human ILC2s is reported, where we demonstrate that Prup3_complex can induce their phenotype switch (↑CD86, ↑S1P1) when cultured in vitro, although more data is needed to understand the implications of these changes in food allergy development.
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- 2022
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8. Corrigendum: Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines
- Author
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Diego Pazos-Castro, Clémence Margain, Zulema Gonzalez-Klein, Marina Amores-Borge, Carmen Yuste-Calvo, Maria Garrido-Arandia, Lucía Zurita, Vanesa Esteban, Jaime Tome-Amat, Araceli Diaz-Perales, and Fernando Ponz
- Subjects
virus-like particles ,antigen delivery ,food allergy ,immunotherapy ,plant biotechnology ,turnip mosaic virus ,Immunologic diseases. Allergy ,RC581-607 - Published
- 2023
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9. Developing an Optical Interferometric Detection Method based biosensor for detecting specific SARS-CoV-2 immunoglobulins in Serum and Saliva, and their corresponding ELISA correlation
- Author
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Murillo, A.M.M., Tomé-Amat, J., Ramírez, Y., Garrido-Arandia, M., Valle, L.G., Hernández-Ramírez, G., Tramarin, L., Herreros, P., Santamaría, B., Díaz-Perales, A., and Holgado, M.
- Published
- 2021
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10. Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines
- Author
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Diego Pazos-Castro, Clémence Margain, Zulema Gonzalez-Klein, Marina Amores-Borge, Carmen Yuste-Calvo, Maria Garrido-Arandia, Lucía Zurita, Vanesa Esteban, Jaime Tome-Amat, Araceli Diaz-Perales, and Fernando Ponz
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virus-like particles ,antigen delivery ,food allergy ,immunotherapy ,plant biotechnology ,turnip mosaic virus ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Virus-like particles (VLPs) have been gaining attention as potential platforms for delivery of cargos in nanomedicine. Although animal viruses are largely selected due to their immunostimulatory capacities, VLPs from plant viruses constitute a promising alternative to be considered. VLPs derived from Turnip mosaic virus (TuMV) have proven to present a tridimensional structure suited to display molecules of interest on their surface, making them interesting tools to be studied in theragnostic strategies. Here, we study their potential in the treatment of food allergy by genetically coupling TuMV-derived VLPs to Pru p 3, one of the most dominant allergens in Mediterranean climates. VLPs-Pru p 3 were generated by cloning a synthetic gene encoding the TuMV coat protein and Pru p 3, separated by a linker, into a transient high-expression vector, followed by agroinfiltration in Nicotiana benthamiana plants. The generated fusion protein self-assembled in planta to form the VLPs, which were purified by exclusion chromatography. Their elongated morphology was confirmed by electron microscopy and their size (~400 nm), and monodispersity was confirmed by dynamic light scattering. Initial in vitro characterization confirmed that they were able to induce proliferation of human immune cells. This proliferative capability was enhanced when coupled with the natural lipid ligand of Pru p 3. The resultant formulation, called VLP-Complex, was also able to be transported by intestinal epithelial cells, without affecting the monolayer integrity. In light of all these results, VLP-Complex was furtherly tested in a mouse model of food allergy. Sublingual administration of VLP-Complex could effectively reduce some serological markers associated with allergic responses in mice, such as anti-Pru p 3 sIgE and sIgG2a. Noteworthy, no associated macroscopic, nephritic, or hepatic toxicity was detected, as assessed by weight, blood urea nitrogen (BUN) and galectin-3 analyses, respectively. Our results highlight the standardized production of allergen-coated TuMV-VLPs in N. benthamiana plants. The resulting formula exerts notable immunomodulatory properties without the need for potentially hazardous adjuvants. Accordingly, no detectable toxicity associated to their administration was detected. As a result, we propose them as good candidates to be furtherly studied in the treatment of immune-based pathologies.
- Published
- 2022
- Full Text
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11. Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages
- Author
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Guadalupe Hernandez-Ramirez, Diego Pazos-Castro, Zulema Gonzalez-Klein, Jose Luis Resuela-Gonzalez, Sergio Fernandez-Bravo, Lucia Palacio-Garcia, Vanesa Esteban, Maria Garrido-Arandia, Jaime Tome-Amat, and Araceli Diaz-Perales
- Subjects
allergic asthma ,Alt a 1 ,TLR4 ,alveolar macrophage ,mouse model ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The mold Alternaria alternata is one of the main sources of asthma exacerbation, being its major allergen, Alt a 1, indispensable for its development. The main objective of this work was to answer two main questions: 1) can Alt a 1 by itself (without any other context) induce an asthmatic profile in vivo?; and 2) Which molecular mechanisms take place during this phenomenon? To answer both questions, we have developed a mouse model of allergic asthma using only Alt a 1 for mice sensitization. We also made use of in-vitro cellular models and computational studies to support some aspects of our hypothesis. Our results showed that Alt a 1 can induce an asthmatic phenotype, promoting tissue remodeling and infiltration of CD45+ cells, especially eosinophils and macrophages (Siglec F+ and F4/80+). Also, we have found that Alt a 1 sensitization is mediated by the TLR4-macrophage axis.
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- 2022
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12. Lipid Ligands and Allergenic LTPs: Redefining the Paradigm of the Protein-Centered Vision in Allergy
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Zulema Gonzalez-Klein, Diego Pazos-Castro, Guadalupe Hernandez-Ramirez, Maria Garrido-Arandia, Araceli Diaz-Perales, and Jaime Tome-Amat
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allergy ,LTP ,sensitization ,animal models ,lipid ligand ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Lipid Transfer Proteins (LTPs) have been described as one of the most prevalent and cross-reactive allergen families in the general population. They are widely distributed among the plant kingdom, as well as in different plant organs ranging from pollen to fruits. Thus, they can initiate allergic reactions with very different outcomes, such as asthma and food allergy. Several mouse models have been developed to unravel the mechanisms that lead LTPs to promote such strong sensitization patterns. Interestingly, the union of certain ligands can strengthen the allergenic capacity of LTPs, suggesting that not only is the protein relevant in the sensitization process, but also the ligands that LTPs carry in their cavity. In fact, different LTPs with pro-allergenic capacity have been shown to transport similar ligands, thus positioning lipids in a central role during the first stages of the allergic response. Here, we offer the latest advances in the use of experimental animals to study the topic, remarking differences among them and providing future researchers a tool to choose the most suitable model to achieve their goals. Also, recent results derived from metabolomic studies in humans are included, highlighting how allergic diseases alter the lipidic metabolism toward a pathogenic state in the individual. Altogether, this review offers a comprehensive body of work that sums up the background evidence supporting the role of lipids as modulators of allergic diseases. Studying the role of lipids during allergic sensitization might broaden our understanding of the molecular events leading to tolerance breakdown in the epithelium, thus helping us to understand how allergy is initiated and established in the individuals.
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- 2022
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13. Virus-like Particles as Vaccines for Allergen-Specific Therapy: An Overview of Current Developments.
- Author
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Berreiros-Hortala, Helena, Vilchez-Pinto, Gonzalo, Diaz-Perales, Araceli, Garrido-Arandia, Maria, and Tome-Amat, Jaime
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VIRUS-like particles ,IMMUNOMODULATORS ,IMMUNOREGULATION ,FOOD allergy ,PLANT viruses - Abstract
Immune engineering and modulation are the basis of a novel but powerful tool to treat immune diseases using virus-like particles (VLPs). VLPs are formed by the viral capsid without genetic material making them non-infective. However, they offer a wide variety of possibilities as antigen-presenting platforms, resulting in high immunogenicity and high efficacy in immune modulation, with low allergenicity. Both animal and plant viruses are being studied for use in the treatment of food allergies. These formulations are combined with adjuvants, T-stimulatory epitopes, TLR ligands, and other immune modulators to modulate or enhance the immune response toward the presented allergen. Here, the authors present an overview of VLP production systems, their immune modulation capabilities, and the applicability of actual VLP-based formulations targeting allergic diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
14. The TNF-like weak inducer of the apoptosis/fibroblast growth factor–inducible molecule 14 axis mediates histamine and platelet-activating factor–induced subcutaneous vascular leakage and anaphylactic shock
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Mendez-Barbero, Nerea, Yuste-Montalvo, Alma, Nuñez-Borque, Emilio, Jensen, Bettina M., Gutiérrez-Muñoz, Carmen, Tome-Amat, Jaime, Garrido-Arandia, María, Díaz-Perales, Araceli, Ballesteros-Martinez, Contanza, Laguna, Jose Julio, Beitia, J.M., Poulsen, Lars K., Cuesta-Herranz, Javier, Blanco-Colio, Luis Miguel, and Esteban, Vanesa
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- 2020
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15. Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTipTM Probes: Application to the Pru p 3-Phytosphingosine System
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Daza, Rafael, primary, Garrido-Arandia, María, additional, Corregidor-Ortiz, Daniel, additional, Pérez, Carla Isabel, additional, Colchero, Luis, additional, Tabraue-Rubio, Raquel, additional, Elices, Manuel, additional, Guinea, Gustavo V., additional, Diaz-Perales, Araceli, additional, and Pérez-Rigueiro, José, additional
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- 2023
- Full Text
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16. Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat
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Khawla Missaoui, Zulema Gonzalez-Klein, Sonia Jemli, Maria Garrido-Arandia, Araceli Diaz-Perales, Jaime Tome-Amat, and Faiçal Brini
- Subjects
Medicine ,Science - Abstract
Non-specific lipid transfer proteins (nsLTPs) are small, cysteine-rich proteins, a part of the pathogenesis-related protein family, and numerous of them act as positive regulators during plant disease resistance, growth, and reproduction. These proteins are involved also in the intracellular transfer of lipids, as well as in plant immune responses. Besides their differences in sequences, they show similar features in their structure. However, they show distinct lipid-binding specificities signifying their various biological roles that dictate further structural study. This study reports the identification, in silico characterization and purification of a novel member of the nsLTP2 protein family from durum wheat, TdLTP2. It was generated and purified using the combination of gel filtration chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). Its identity was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry (MALDI-TOF). TdLTP2 had been expressed in different stress to detect its localization; therefore, fluor-immunolocalization studies accomplished this data. In this approach, to assess the allergenicity of TdLTP2, thirty patients with baker’s asthma were enrolled and ELISA to detect the presence of specific IgE antibodies tested their sera. Moreover, the lipid-binding properties of TdLTP2 were examined in vitro and validated using a molecular docking study. In summary, our results demonstrate a new addition of member in plant nsLTPs family, TdLTP2, which can develop a better understanding about its biological functions and shed light on future applications.
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- 2022
17. Plant lipid transfer proteins' ligand enhances allergic sensitization to non‐related proteins in murine models
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Tome‐Amat, Jaime, primary, Gonzalez‐Klein, Zulema, additional, Pazos‐Castro, Diego, additional, Resuela‐Gonzalez, Jose Luis, additional, Vilchez‐Pinto, Gonzalo, additional, Berreiros‐Hortala, Helena, additional, Palacio‐Garcia, Lucia, additional, Fernandez‐Bravo, Sergio, additional, Esteban, Vanesa, additional, Diaz‐Perales, Araceli, additional, and Garrido‐Arandia, Maria, additional
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- 2023
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18. Effect of pre- and post-weaning dietary supplementation with arginine and glutamine on rabbit performance and intestinal health
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Rebeca Delgado, Rodrigo Abad-Guamán, Nuria Nicodemus, Araceli Diaz-Perales, Javier García, Rosa Carabaño, and David Menoyo
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Arginine ,Glutamine ,Rabbit ,Gut health ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background The purpose of the present study was to assess if the exposure to glutamine (Gln), arginine (Arg) or their combination from pregnancy, through the maternal diet, to a post weaning supplemented diet, can stimulate litter performance, gut development and immune function. To this end does and their litters were fed the same basal diet no supplemented (control C), or supplemented with 0.4% Gln, 0.4% Arg, or 0.4 Gln + 0.4 Arg. Rabbits were weaned at 25 d of age and fed the same experimental diet as their mothers for 10 additional days (35 d of age). Bacterial translocation to mesenteric lymph nodes (MLN) at 6 d of age and intestinal histology, enzymatic activity, phenotypical and functional analysis of intraepithelial lymphocytes (IEL) from the appendix were determined at 6, 25 and 35 d of age. Results No significant differences on animal performance or mortality rates were observed among dietary treatments. However, kits from rabbit does supplemented with Gln tended (P ≤ 0.10) to reduce the translocation of total number of both aerobic and facultative anaerobic bacteria to the MLN. Also, rabbits fed the Gln supplemented diets maintained intestinal villous height at weaning compared to the non-supplemented diets (P
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- 2019
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19. A relevant IgE-reactive 28 kDa protein identified from Salsola kali pollen extract by proteomics is a natural degradation product of an integral 47 kDa polygalaturonase
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Mas, Salvador, Oeo-Santos, Carmen, Cuesta-Herranz, Javier, Díaz-Perales, Araceli, Colás, Carlos, Fernández, Javier, Barber, Domingo, Rodríguez, Rosalía, de los Ríos, Vivian, Barderas, Rodrigo, and Villalba, Mayte
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- 2017
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20. Plant lipid transfer proteins' ligand enhances allergic sensitization to non‐related proteins in murine models.
- Author
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Tome‐Amat, Jaime, Gonzalez‐Klein, Zulema, Pazos‐Castro, Diego, Resuela‐Gonzalez, Jose Luis, Vilchez‐Pinto, Gonzalo, Berreiros‐Hortala, Helena, Palacio‐Garcia, Lucia, Fernandez‐Bravo, Sergio, Esteban, Vanesa, Diaz‐Perales, Araceli, and Garrido‐Arandia, Maria
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LIPID transfer protein ,PLANT lipids ,PROTEIN models ,CELL migration ,ALLERGIES ,FILAGGRIN - Abstract
The article discusses the role of plant lipid transfer proteins' (LTPs) ligand in allergic sensitization. The study found that the LTPs' ligand enhances allergic sensitization to unrelated proteins in murine models. The ligand was shown to induce immune cell migration and promote local inflammation, leading to systemic allergic sensitization. The findings highlight the importance of the ligand in the development of allergic reactions and suggest that the phosphorylation of the ligand is key to inducing allergic sensitization. Further research is needed to fully understand these mechanisms. The study was supported by various grants and there are no conflicts of interest declared by the authors. [Extracted from the article]
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- 2024
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21. Alternaria as an Inducer of Allergic Sensitization
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Guadalupe Hernandez-Ramirez, Domingo Barber, Jaime Tome-Amat, Maria Garrido-Arandia, and Araceli Diaz-Perales
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mold asthma ,Alternaria alternata ,Alternaria allergens ,Alt a 1 ,Biology (General) ,QH301-705.5 - Abstract
Alternaria alternata is a saprophytic mold whose spores are disseminated in warm dry air, the typical weather of the Mediterranean climate region (from 30° to 45°), with a peak during the late summer and early autumn. Alternaria spores are known to be biological contaminants and a potent source of aeroallergens. One consequence of human exposure to Alternaria is an increased risk of developing asthma, with Alt a 1 as its main elicitor and a marker of primary sensitization. Although the action mechanism needs further investigation, a key role of the epithelium in cytokine production, TLR-activated alveolar macrophages and innate lymphoid cells in the adaptive response was demonstrated. Furthermore, sensitization to A. alternata seems to be a trigger for the development of co-sensitization to other allergen sources and may act as an exacerbator of symptoms and an elicitor of food allergies. The prevalence of A. alternata allergy is increasing and has led to expanding research on the role of this fungal species in the induction of IgE-mediated respiratory diseases. Indeed, recent research has allowed new perspectives to be considered in the assessment of exposure and diagnosis of fungi-induced allergies, although more studies are needed for the standardization of immunotherapy formulations.
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- 2021
- Full Text
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22. Statistical Study of Low-Intensity Single-Molecule Recognition Events Using DeepTip TM Probes: Application to the Pru p 3-Phytosphingosine System.
- Author
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Daza, Rafael, Garrido-Arandia, María, Corregidor-Ortiz, Daniel, Pérez, Carla Isabel, Colchero, Luis, Tabraue-Rubio, Raquel, Elices, Manuel, Guinea, Gustavo V., Diaz-Perales, Araceli, and Pérez-Rigueiro, José
- Subjects
LIPID transfer protein ,ATOMIC force microscopes ,BIOMOLECULES ,PLANT lipids ,HYDROPHOBIC interactions - Abstract
The interaction between the plant lipid transfer protein Pru p 3 and phytosphingosine was assessed using an atomic force microscope. Phytosphingosine was covalently immobilized on DeepTip
TM probes and Pru p 3 on MicroDeckTM functionalized substrates. Single-molecular interaction events between both molecules were retrieved and classified and the distribution for each one of the identified types was calculated. A success rate of over 70% was found by comparing the number of specific Pru p 3-phytosphingosine interaction events with the total number of recorded curves. The analysis of the distribution established among the various types of curves was further pursued to distinguish between those curves that can mainly be used for assessing the recognition between phytosphingosine (sensor molecule) and Pru p 3 (target molecule) in the context of affinity atomic force microscopy, and those that entail details of the interaction and might be employed in the context of force spectroscopy. The successful application of these functionalized probes and substrates to the characterization of the low-intensity hydrophobic interaction characteristic of this system is a clear indication of the potential of exploiting this approach with an extremely wide range of different biological molecules of interest. The possibility of characterizing molecular assembly events with single-molecule resolution offers an advantageous procedure to plough into the field of molecular biomimetics. [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
23. Plant non-specific lipid transfer proteins: An overview
- Author
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Khawla Missaoui, Zulema Gonzalez-Klein, Diego Pazos-Castro, Guadalupe Hernandez-Ramirez, Maria Garrido-Arandia, Faical Brini, Araceli Diaz-Perales, Jaime Tome-Amat, Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie), Comunidad de Madrid, Universidad Politécnica de Madrid, Banco Santander, European Commission, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación (España), Gonzalez-Klein, Zulema [0000-0003-3762-3781], Pazos-Castro, Diego [0000-0002-0686-5131], Hernandez-Ramirez, Guadalupe [0000-0003-3031-6754], Garrido-Arandia, Maria [0000-0001-6114-5754], Brini, Faical [0000-0002-8435-381X], Diaz-Perales, Araceli [0000-0002-1093-3627], Tome-Amat, Jaime [0000-0003-4442-3649], Gonzalez-Klein, Zulema, Pazos-Castro, Diego, Hernandez-Ramirez, Guadalupe, Garrido-Arandia, Maria, Brini, Faical, Diaz-Perales, Araceli, and Tome-Amat, Jaime
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Lipid transfer protein ,Physiology ,LTPs function ,Genetics ,Plant Development ,Lipid ligand ,Plant Science ,Antigens, Plant ,Plants ,Plant metabolism ,Lipids ,Plant Proteins - Abstract
13 Pág., Plant non-specific lipid transfer proteins (nsLTPs) are usually defined as small, basic proteins, with a wide distribution in all orders of higher plants. Structurally, nsLTPs contain a conserved motif of eight cysteines, linked by four disulphide bonds, and a hydrophobic cavity in which the ligand is housed. This structure confers stability and enhances the ability to bind and transport a variety of hydrophobic molecules. Their highly conserved structural resemblance but low sequence identity reflects the wide variety of ligands they can carry, as well as the broad biological functions to which they are linked to, such as membrane stabilization, cell wall organization and signal transduction. In addition, they have also been described as essential in resistance to biotic and abiotic stresses, plant growth and development, seed development, and germination. Hence, there is growing interest in this family of proteins for their critical roles in plant development and for the many unresolved questions that need to be clarified, regarding their subcellular localization, transfer capacity, expression profile, biological function, and evolution., KM was supported through funding by the Ministry of Higher Education and Scientific Research of Tunisia (CP_2019–2022), ZGK was funded by the Community of Madrid (FOODAL-CM_S2018/BAAA-4574). DPC was granted by Universidad Politécnica de Madrid and Banco Santander for a predoctoral Programa Propio grant. GHR was funded by European Commission (H2020-NMBP-X-KET-2017-768641 AllerScreening). JTA was funded by Instituto de Salud Carlos III (ISCIII) co-founded by FEDER Thematic Networks and Cooperative Research Centers: ARADYAL (RD16/0006/0003). This work was also funded by the Spanish Ministry of Science and Innovation (LISSENTRA; PID2020-113629RB-I00).
- Published
- 2022
24. Real-life evaluation of molecular multiplex IgE test methods in the diagnosis of pollen associated food allergy
- Author
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Luisa Diem, Bellinda Neuherz, Johanna Rohrhofer, Larissa Koidl, Riccardo Asero, Knut Brockow, Araceli Diaz Perales, Margaretha Faber, Julia Gebhardt, María José Torres, Erika Jensen‐Jarolim, Sonja Zehetmayer, Eva Untersmayr, European Commission, Rohrhofer, Johanna [0000-0002-2783-2099], Koidl, Larissa [0000-0001-9974-7929], Asero, Riccardo [0000-0002-8277-1700], Brockow, Knut [0000-0002-2775-3681], Diaz Perales, Araceli [0000-0002-1093-3627], Faber, Margaretha [0000-0002-1277-5052], Gebhardt, Julia [0000-0002-5529-7337], Torres, María José [0000-0001-5228-471X], Jensen-Jarolim, Erika [0000-0003-4019-5765], Zehetmayer, Sonja [0000-0001-6863-7997], Untersmayr, Eva [0000-0002-1963-499X], Rohrhofer, Johanna, Koidl, Larissa, Asero, Riccardo, Brockow, Knut, Diaz Perales, Araceli, Faber, Margaretha, Gebhardt, Julia, Torres, María José, Jensen-Jarolim, Erika, Zehetmayer, Sonja, and Untersmayr, Eva
- Subjects
Immunology ,Allergens ,Immunoglobulin E ,Profilins ,Multiplex testing ,Food allergy ,Immunology and Allergy ,Humans ,Pollen ,Molecular diagnosis ,Pollen food syndrome ,Food Hypersensitivity ,Skin Tests - Abstract
13 Pág., Diagnosis of food allergies is challenging, as combining information from specific IgE (sIgE)-sensitization pattern and skin prick tests (SPTs) with clinical history is necessary for a personalized management of allergic patients. The aim of this study was to compare two molecular tests, the ImmunoCAP ISAC (ISAC) and the Allergy Explorer, version 2 (ALEX2 ) in the context of pollen food syndrome (PFS) diagnosis in a real-life scenario, to assess the benefit of multiplex testing in PFS patients., This work was financially supported by the H2020 EU project “AllerScreening” (H2020-NMBP-X-KET-2017-768641). EJJ was supported by the Danube Allergy Research Cluster, #08, Vienna and Krems, Austria
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- 2022
25. Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat
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Khawla Missaoui, Zulema Gonzalez-Klein, Sonia Jemli, Maria Garrido-Arandia, Araceli Diaz-Perales, Jaime Tome-Amat, Faiçal Brini, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Comunidad de Madrid, Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie), Gonzalez-Klein, Zulema [0000-0003-3762-3781], Jemli, Sonia [0000-0001-6265-581X], Garrido-Arandia, Maria [0000-0001-6114-5754], Diaz-Perales, Araceli [0000-0002-1093-3627], Tome-Amat, Jaime [0000-0003-4442-3649], Brini, Faiçal [0000-0002-8435-381X], Gonzalez-Klein, Zulema, Jemli, Sonia, Garrido-Arandia, Maria, Diaz-Perales, Araceli, Tome-Amat, Jaime, and Brini, Faiçal
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Electrophoresis ,Molecular Docking Simulation ,Polyacrylamide Gel ,Multidisciplinary ,Proteins ,Electrophoresis, Polyacrylamide Gel ,Allergens ,Carrier Proteins ,Lipids ,Triticum ,Plant Proteins - Abstract
17 Pág., Non-specific lipid transfer proteins (nsLTPs) are small, cysteine-rich proteins, a part of the pathogenesis-related protein family, and numerous of them act as positive regulators during plant disease resistance, growth, and reproduction. These proteins are involved also in the intracellular transfer of lipids, as well as in plant immune responses. Besides their differences in sequences, they show similar features in their structure. However, they show distinct lipid-binding specificities signifying their various biological roles that dictate further structural study. This study reports the identification, in silico characterization and purification of a novel member of the nsLTP2 protein family from durum wheat, TdLTP2. It was generated and purified using the combination of gel filtration chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). Its identity was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry (MALDI-TOF). TdLTP2 had been expressed in different stress to detect its localization; therefore, fluor-immunolocalization studies accomplished this data. In this approach, to assess the allergenicity of TdLTP2, thirty patients with baker's asthma were enrolled and ELISA to detect the presence of specific IgE antibodies tested their sera. Moreover, the lipid-binding properties of TdLTP2 were examined in vitro and validated using a molecular docking study. In summary, our results demonstrate a new addition of member in plant nsLTPs family, TdLTP2, which can develop a better understanding about its biological functions and shed light on future applications., This work was funded by the Spanish Ministry of Science and Innovation through the project LISENTRA, granted by the Spanish Research State Agency (PID2020-113629RBI00/AEI/10.13039/501100011033), the Comunidad de Madrid (FOODAL-CM: S2018/BAA4574) Spain, and a grant from the Ministry of Higher Education, and Scientific Research of Tunisia, and a grant from. There was no additional external funding received for this study
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- 2021
26. Immunological Changes Induced in Peach Allergy Patients with Systemic Reactions by Pru p 3 Sublingual Immunotherapy
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Palomares, Francisca, Gomez, Francisca, Bogas, Gador, Campo, Paloma, Perkins, James Richard, Diaz‐Perales, Araceli, Rodriguez, Maria J., Prieto, Ana, Barber, Domingo, Torres, María J., and Mayorga, Cristobalina
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- 2018
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27. Current (Food) Allergenic Risk Assessment: Is It Fit for Novel Foods? Status Quo and Identification of Gaps
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Mazzucchelli, Gabriel, Holzhauser, Thomas, Cirkovic Velickovic, Tanja, Diaz‐Perales, Araceli, Molina, Elena, Roncada, Paola, Rodrigues, Pedro, Verhoeckx, Kitty, and Hoffmann‐Sommergruber, Karin
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- 2018
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28. Effect of pre- and post-weaning dietary supplementation with arginine and glutamine on rabbit performance and intestinal health
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Delgado, Rebeca, Abad-Guamán, Rodrigo, Nicodemus, Nuria, Diaz-Perales, Araceli, García, Javier, Carabaño, Rosa, and Menoyo, David
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- 2019
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29. Corrigendum: Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines
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Pazos-Castro, Diego, primary, Margain, Clémence, additional, Gonzalez-Klein, Zulema, additional, Amores-Borge, Marina, additional, Yuste-Calvo, Carmen, additional, Garrido-Arandia, Maria, additional, Zurita, Lucía, additional, Esteban, Vanesa, additional, Tome-Amat, Jaime, additional, Diaz-Perales, Araceli, additional, and Ponz, Fernando, additional
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- 2023
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30. 6th International Symposium on Molecular Allergology (ISMA)
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Christiane Hilger, Kyra Swiontek, Jörg Fischer, François Hentges, Christiane Lehners, Martine Morisset, Bernadette Eberlein, Tilo Biedermann, Markus Ollert, Sabrina Wildner, Teresa Stemeseder, Regina Freier, Peter Briza, Roland Lang, Eva Batanero, Mayte Villalba, Jonas Lidholm, Thomas Hawranek, Fatima Ferreira, Hans Brandstetter, Gabriele Gadermaier, Philippe Moingeon, Rachel Groeme, Julien Bouley, Véronique Bordas, Maxime Le Mignon, Laetitia Bussières, Aurélie Lautrette, Laurent Mascarell, Vincent Lombardi, Véronique Baron-Bodo, Henri Chabre, Thierry Batard, Emmanuel Nony, Karine Marafigo De Amicis, Alexandra Sayuri Watanabe, Daniele Danella Figo, José Roberto Aparecido Dos Santos-Pinto, Mario Sergio Palma, Fabio Fernandes Morato Castro, Jorge Kalil, Therese Wohlschlager, Fatima Ferreira-Briza, Keity Souza Santos, Margaretha Faber, Athina Van Gasse, Vito Sabato, Margo M. Hagendorens, Chris H. Bridts, Luc S. De Clerck, Araceli Diaz Perales, Didier Ebo, Petra Zavadakova, Aurélie Buchwalder, Fabien Rebeaud, Iwan Märki, Barbara Gepp, Nina Lengger, Christian Möbs, Wolfgang Pfützner, Christian Radauer, Barbara Bohle, Clovis Eduardo Galvao, Jose Roberto Aparecido Santos-Pinto, Christian Schwager, Skadi Kull, Frauke Schocker, Jochen Behrends, Wolf-Meinhard Becker, Uta Jappe, Carla Mastrorilli, Salvatore Tripodi, Carlo Caffarelli, Riccardo Asero, Arianna Dondi, Giampaolo Ricci, Carlotta Povesi Dascola, Elisabetta Calamelli, Andrea Di Rienzo Businco, Annamaria Bianchi, Tullio Frediani, Carmen Verga, Iride Dello Iacono, Diego Peroni, Giuseppe Pingitore, Roberto Bernardini, Paolo Maria Matricardi, Heidi Hofer, Claudia Asam, Michael Hauser, Martin Himly, Christof Ebner, Pierrick Lemoine, Karine Jain, Kathy Abiteboul, Monica Arvidsson, Sabina Rak, Inês Mota, Filipe Benito Garcia, Angela Gaspar, Cristina Arêde, Susana Piedade, Graça Sampaio, Graça Pires, Luís Miguel Borrego, Cristina Santa-Marta, Mário Morais-Almeida, Florin-Dan Popescu, Mariana Vieru, Florin-Adrian Secureanu, Rosa Anita Rodrigues Fernandes, Isabel Carrapatoso, Raquel Gomes, Celso Pereira, Ana Todo-Bom, María Cecilia Martín Fernández De Basoa, Javier Barrios Regio, Juan De Castro Cordova, Antón Fernández Ferreiro, Olympia Tsilochristou, Serena Perna, Alina Schwarz, Alexander Rohrbach, Antonio Cappella, Laura Hatzler, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Raffael D’amelio, Ulrich Wahn, Thomas Keil, Susanne Lau, Pol André Apoil, Claire Mailhol, Anne Broué-Chabbert, Agnès Juchet, Alain Didier, Elodie Carrer, Thomas Lanot, Antoine Blancher, Almedina Kurtaj, Christoph Hillebrand, Gerda Fichtinger, Martin Danzer, Christian Gabriel, Theresa Thalhamer, Sandra Scheiblhofer, Josef Thalhamer, Richard Weiss, Martin Wolf, Ulrike Pichler, Teresa Twaroch, Hidenori Yokoi, Toshiro Takai, Alain Didierlaurent, Adriano Mari, Heidrun Behrendt, Angela Neubauer, Frank Stolz, Fátima Ferreira, Michael Wallner, Sara Carvalho, Tatiana Lourenço, Joana Cosme, Fátima Cabral Duarte, Amélia Spínola Santos, Ana Célia Costa, Manuel Pereira Barbosa, Eva Klinglmayr, Bettina Schweidler, Lisa Lueftenegger, Stephanie Moser, Patrick Doppler, Gertie J. Oostingh, Arne Bathke, Joerg Zumbach, Petr Panzner, Martina Vachova, Tomas Vlas, Marek Maly, Daniela Posa, Stephanie Hofmaier, Philippe Stock, Linus Grabenhenrich, Kuan-Wei Chen, Yvonne Resch, Susanne Vrtala, Rudolf Valenta, Tamar Abramidze, Nino Lomidze, Maia Gotua, Austeja Dapkeviciute, Ruta Einikyte, Jolita Norkuniene, Laima Skrickiene, Asta Miskiniene, Violeta Kvedariene, Maximilian Schiener, Carmen Moreno-Aguilar, Gunilla Pietsch, Mareike Mc Intyre, Lea Schwarze, Dennis Rußkamp, Edzard Spillner, Ulf Darsow, Carsten Schmidt-Weber, Simon Blank, Cyril Longé, Andrea Brazdova, Jean-Louis Brunet, Claire Schwartz, Bruno Girodet, François Lavaud, Joelle Birnbaum, Nhân Pham Thi, Magalie Duchateau, Julia Chamot-Rooke, Laurence Guilloux, Marie-Ange Selva, Rémy Couderc, Hélène Sénéchal, Jean-Pierre Sutra, Pascal Poncet, Steffen Augustin, Linda Pump, Martin Wald, Thomas Eichhorn, Frank Fischer, Christoph Willers, Michaela Miehe, Melanie Plum, Sara Wolf, Frederic Jabs, Tim Raiber, Frank Bantleon, Henning Seismann, Thilo Jakob, Danijela Apostolovic, Anh Thu Tran, Sara Sanchez-Vidaurre, Tanja Cirkovic Velickovic, Maria Starkhammar, Carl Hamsten, Marianne Van Hage, Pawel Dubiela, Piotr Humeniuk, Sabine Pfeifer, Merima Bublin, Tomasz Borowski, Karin Hoffmann-Sommergruber, Martie C. M. Verschuren, Shanna Bastiaan-Net, Defien Depoortere, Kay Foetisch, Stephan Scheurer, Harry J Wichers, Theo Noij, Nikki M.E. Van Uden, Karel Vandenberghe, Harry J. Wichers, Theo H. M. Noij, Anargyros Roulias, Maria Alejandra Parigiani, Linda Ahammer, Sarina Grutsch, Martin Tollinger, Raquel Moya, Mª Angeles López-Matas, Raquel Reyes, Jerónimo Carnés, Colette Larré, Hélène Rogniaux, Roberta Lupi, Sandra Denery-Papini, Isabel Maria Pablos, Stephanie Eichhorn, Yoan Machado, Jung-Won Park, Naveen Arora, Stefan Vieths, Charlene Tanaka, Florence Pineau, Martine Drouet, Etienne Beaudouin, Susan Altenbach, Hamza Mameri, Chantal Brossard, Jean Charles Gaudin, Denise Anne Moneret-Vautrin, Evelyne Paty, Olivier Tranquet, Stefania Masci, Denise-Anne Moneret-Vautrin, Arnd Petersen, Marisa Böttger, Sandra Rennert, Susanne Krause, Martin Ernst, Thomas Gutsmann, Johann Bauer, Buko Lindner, Stef Koppelman, Shyamali Jayasena, Dion Luykx, Erik Schepens, Govardus De Jong, Tom Isleib, Julie Nordlee, Joe Baumert, Steve Taylor, Soheila Maleki, Chiara Palladino, Sofía Sirvent, Alba Angelina, Thomas Eiwegger, Oscar Palomares, Heimo Breiteneder, Mathilde Claude, Grégory Bouchaud, Marie Bodinier, Robin Korte, Julia Bräcker, Jens Brockmeyer, Rie Satoh, Reiko Teshima, Angelika Tscheppe, Dieter Palmberger, Reingard Grabherr, Marianne Raith, Linda Sonnleitner, Doris Zach, Konrad Woroszylo, Margit Focke-Tejkl, Herbert Wank, Thorsten Graf, Annette Kuehn, Ines Swoboda, Sara Huber, Fabienne Gay-Crosier, Dominika Polak, Birgit Nagl, Claudia Kitzmüller, Nazanin Samadi, Rene Geyeregger, Beatrice Jahn-Schmid, Ariel Gomez, Jaana Haka, Liisa Hattara, Marika Heikkinen, Merja H Niemi, Juha Rouvinen, Petri Saviranta, Pekka Mattila, Kristiina Takkinen, Marja-Leena Laukkanen, Isabel Pablos, Bianca Kastner, Mira Silar, Julij Selb, Rok Kogovsek, Mitja Kosnik, Peter Korosec, Leticia Pestana, Alcinda Campos Melo, Ana Mendes, Maria Elisa Pedro, Maria Conceição Pereira Santos, Françoise Bienvenu, Claire Goursaud, Lorna Garnier, Sandrine Jacquenet, Michaël Degaud, Sébastien Viel, Annick Barre, Pierre Rougé, Jacques Bienvenu, Joana Vitte, Amel Bensalah, Isabelle Cleach, Laurent Mousseau, Chantal Agabriel, Valérie Liabeuf, Joëlle Birnbaum, Jean-Louis Mège, James Gardner, Minal Gandhi, Harsha Kariyawasam, Giuseppina Rotiroti, Frederico Regateiro, Emília Faria, Johannes Martin Schmid, Ronald Dahl, Hans Juergen Hoffmann, Letícia Pestana, Diana Silva, Teresa Vieira, Ana Maria Pereira, André Moreira, Luís Delgado, Sara Prates, Cátia Alves, Elena Finelli, Paula Leiria Pinto, Bárbara Kong Cardoso, Cíntia Cruz, Filipa Semedo, Elza Tomaz, Filipe Inácio, Santanu Maity, Ivona Baricevic-Jones, Justin T. Marsh, Phil E. Johnson, Anuradha Balasundaram, Anya-May Hope, Aafke Taekema, Angela Simpson, Aida Semic-Jusufagic, E. N. Clare Mills, Gourdon Dubois Nelly, Sellam Laetitia, Pereira Bruno, Michaud Elodie, Messaoudi Khaled, Evrard Bertrand, Fauquert Jean-Luc, Richard E. Goodman, Elena Rodríguez Plata, Luis Amaral, Borja Bartolomé, Alice Coimbra, Jose L Placido, Carmen Saviana Ganea, Carol Ann Costello, Martin Sorensen, Clare Mills, Adrian Rogers, Aage Otherhals, Tanja Kalic, Isabella Ellinger, Eva Waltl, Verena Niederberger-Leppin, Dawid Szczepankiewicz, Ewa Pruszynska-Oszmalek, Marek Skrzypski, Krzysztof W. Nowak, Aleksandra Szczepankiewicz, Gwang-Cheon Jang, Iva Markovic, Andreas Borowski, Tina Vetter, Andreas Wohlmann, Michael Kuepper, Karlheinz Friedrich, Ibon Eguiluz Gracia, Anthony Bosco, Ralph Dollner, Guro Reinholt Melum, Anya C Jones, Maria Lexberg, Patrick G Holt, Espen Sønderaal Bækkevold, Frode Lars Jahnsen, Paulina Sobkowiak, Marta Rachel, Beata Narozna, Dorota Jenerowicz, Witold Swiatowy, Anna Breborowicz, Reinhard Nestelbacher, and Hiroyuki Fukui
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Table of contents ORAL ABSTRACTS Symposium 1: Biochemistry, structure and environment of the allergen: what makes a protein an allergen? O1 Two cell-membrane peptidases carrying galactose-alpha-1,3-galactose are implicated in delayed anaphylactic reactions upon pork kidney ingestion in patients with IgE-antibodies to alpha-Gal Christiane Hilger, Kyra Swiontek, Jörg Fischer, François Hentges, Christiane Lehners, Martine Morisset, Bernadette Eberlein, Tilo Biedermann, Markus Ollert O2 Structure solution of Pla l 1 suggests similar folding of Ole e 1-like family members but distinct immunological properties Sabrina Wildner, Teresa Stemeseder, Regina Freier, Peter Briza, Roland Lang, Eva Batanero, Mayte Villalba, Jonas Lidholm, Thomas Hawranek, Fatima Ferreira, Hans Brandstetter, Gabriele Gadermaier Symposium 2: New allergen molecules in the spotlight O3 Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed (Ambrosia artemisiifolia) Philippe Moingeon, Rachel Groeme, Julien Bouley, Véronique Bordas, Maxime Le Mignon, Laetitia Bussières, Aurélie Lautrette, Laurent Mascarell, Vincent Lombardi, Véronique Baron-Bodo, Henri Chabre, Thierry Batard, Emmanuel Nony O4 Production and characterization of polybia paulista recombinant antigen 5: a valuable diagnostic tool Karine Marafigo De Amicis, Alexandra Sayuri Watanabe, Daniele Danella Figo, José Roberto Aparecido Dos Santos-Pinto, Mario Sergio Palma, Fabio Fernandes Morato Castro, Jorge Kalil, Therese Wohlschlager, Peter Briza, Sabrina Wildner, Fatima Ferreira-Briza, Gabriele Gadermaier, Keity Souza Santos Symposium 3: Progress in molecular and cellular diagnosis O5 Basophil activation test with recombinant Pru p 3; identifying genuine peach allergic patients Margaretha Faber, Athina Van Gasse, Vito Sabato, Margo M. Hagendorens, Chris H. Bridts, Luc S. De Clerck, Araceli Diaz Perales, Didier Ebo O6 Nanofluidic technology enables rapid, near-patient quantification of allergen-specific IgE Petra Zavadakova, Aurélie Buchwalder, Fabien Rebeaud, Iwan Märki Symposium 4: Relevance of molecular diagnostics for intervention and treatment O7 Longitudinal analysis of Bet v 1-specific epitope repertoires during birch pollen immunotherapy Barbara Gepp, Nina Lengger, Christian Möbs, Wolfgang Pfützner, Christian Radauer, Barbara Bohle O8 A natural CCD-free tool: is polistes sp. venom suitable for polybia paulista diagnosis and therapy? Karine Marafigo De Amicis, Alexandra Sayuri Watanabe, Clovis Eduardo Galvao, Daniele Danella Figo, Jose Roberto Aparecido Santos-Pinto, Mario Sergio Palma, Fabio Fernandes Morato Castro, Jorge Kalil, Fatima Ferreira, Gabriele Gadermaier, Keity Souza Santos Symposium 5: The advent of molecular allergology in epidemiology O9 Peanut oleosins: from identification to diagnostic testing Christian Schwager, Skadi Kull, Frauke Schocker, Jochen Behrends, Wolf-Meinhard Becker, Uta Jappe O10 Endotypes of oral allergy syndrome in childhood: a molecular diagnostic approach Carla Mastrorilli, Salvatore Tripodi, Carlo Caffarelli, Riccardo Asero, Arianna Dondi, Giampaolo Ricci, Carlotta Povesi Dascola, Elisabetta Calamelli, Andrea Di Rienzo Businco, Annamaria Bianchi, Tullio Frediani, Carmen Verga, Iride Dello Iacono, Diego Peroni, Giuseppe Pingitore, Roberto Bernardini, Paolo Maria Matricardi Symposium 6: Molecular AIT: which approaches will make it to market? O11 Mbc4: an innovative molecule to tackle birch pollen and concomitant food allergies Heidi Hofer, Claudia Asam, Michael Hauser, Peter Briza, Martin Himly, Christof Ebner, Fatima Ferreira O12 Challenges and solutions associated with the production of recombinant Bet v 1 allergen as a therapeutic protein Emmanuel Nony, Maxime Le Mignon, Pierrick Lemoine, Karine Jain, Kathy Abiteboul, Monica Arvidsson, Sabina Rak, Philippe Moingeon Clinical Cases: Breakthroughs and headaches from CRD: interactive session CC1 Anaphylaxis caused by lipid transfer proteins: a complex clinical pattern syndrome Inês Mota, Filipe Benito Garcia, Angela Gaspar, Cristina Arêde, Susana Piedade, Graça Sampaio, Graça Pires, Luís Miguel Borrego, Cristina Santa-Marta, Mário Morais-Almeida CC2 IgE sensitization profile in a patient with asteraceae pollen-exotic fruits association Florin-Dan Popescu, Mariana Vieru, Florin-Adrian Secureanu CC3 Food-dependent: exercise induced anaphylaxis. Which component to blame? Rosa Anita Rodrigues Fernandes, Isabel Carrapatoso, Raquel Gomes, Celso Pereira, Ana Todo-Bom CC4 Anaphylaxis to intravenous iron preparations in a patient that tolerates oral administration María Cecilia Martín Fernández De Basoa, Javier Barrios Regio, Juan De Castro Cordova, Antón Fernández Ferreiro CC5 IgE sensitization pattern in an adult patient with oral allergy syndrome to peanuts and pollinosis from southern Romania Florin-Dan Popescu, Mariana Vieru, Florin-Adrian Secureanu CC6 Evidence of specific IgE to plant-derived cross-reactive carbohydrate determinant in a patient with delayed anaphylaxis to red meat Mariana Vieru, Florin-Dan Popescu, Florin-Adrian Secureanu POSTER PRESENTATIONS Poster Session 1: Molecular allergology and epidemiology P1 Atopic children produce stronger and more frequent IgG responses than non-atopic children: longitudinal data from the German MAS birth cohort Olympia Tsilochristou, Serena Perna, Alina Schwarz, Alexander Rohrbach, Antonio Cappella, Laura Hatzler, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Raffael D’amelio, Ulrich Wahn, Thomas Keil, Susanne Lau, Paolo Maria Matricardi P2 The IgG sensitization profiles against 112 allergenic components support the absence of a protective role of IgG in allergic individuals, outside of the context of SIT Pol André Apoil, Claire Mailhol, Anne Broué-Chabbert, Agnès Juchet, Alain Didier, Elodie Carrer, Thomas Lanot, Antoine Blancher P3 The immune response against the timothy grass pollen allergen Phl p 5 in non-allergic humans Almedina Kurtaj, Christoph Hillebrand, Gerda Fichtinger, Martin Danzer, Christian Gabriel, Theresa Thalhamer, Sandra Scheiblhofer, Josef Thalhamer, Richard Weiss P4 Analyzing the cross-reactivity profile of the major ragweed allergen Amb a 1 Martin Wolf, Michael Hauser, Ulrike Pichler, Teresa Twaroch, Gabriele Gadermaier, Christof Ebner, Hidenori Yokoi, Toshiro Takai, Alain Didierlaurent, Adriano Mari, Peter Briza, Heidrun Behrendt, Angela Neubauer, Frank Stolz, Fátima Ferreira, Michael Wallner P5 LTP (Pru p 3) sensitisation in skin prick test: which means in clinical practice? Sara Carvalho, Tatiana Lourenço, Joana Cosme, Fátima Cabral Duarte, Amélia Spínola Santos, Ana Célia Costa, Manuel Pereira Barbosa P6 IgE profiles, allergen exposure and lifestyle of 501 Austrian pupils: investigation of influences on the development of allergic sensitizations Teresa Stemeseder, Eva Klinglmayr, Bettina Schweidler, Lisa Lueftenegger, Stephanie Moser, Patrick Doppler, Roland Lang, Martin Himly, Gertie J. Oostingh, Arne Bathke, Joerg Zumbach, Thomas Hawranek, Gabriele Gadermaier P7 Molecular profiles of sensitization to perennial inhalant allergens in a middle European region Petr Panzner, Martina Vachova, Tomas Vlas, Marek Maly P8 Evolution of the IgE response to house dust mite allergen molecules in childhood Daniela Posa, Serena Perna, Stephanie Hofmaier, Laura Hatzler, Alexander Rohrbach, Carl-Peter Bauer, Ute Hoffmann, Johannes Forster, Fred Zepp, Antje Schuster, Philippe Stock, Ulrich Wahn, Linus Grabenhenrich, Thomas Keil, Susanne Lau, Kuan-Wei Chen, Yvonne Resch, Susanne Vrtala, Rudolf Valenta, Paolo Maria Matricardi P9 Tropomyosin (Pen a1): to include or not to include in skin prick testing? Joana Cosme, Sara Carvalho, Tatiana Lourenço, Amélia Spínola Santos, Manuel Pereira Barbosa Immunoallergy Department - Hospital de Santa Maria – Centro Hospitalar Lisboa Norte, Lisbon, Portugal, Lisbon, Portugal; Immunoallergy Department - Hospital de Santa Maria – Centro Hospitalar Lisboa Norte, Lisbon, Portugal; Faculdade de Medicina de Lisboa, Lisbon, Portugal P10 Component-resolved IgE profiles in Georgian patients Tamar Abramidze, Nino Lomidze, Maia Gotua P11 Cross reactivity between food and pollen allergens in Lithuania according to spIgE evaluation Austeja Dapkeviciute, Ruta Einikyte, Jolita Norkuniene, Laima Skrickiene, Asta Miskiniene, Violeta Kvedariene P12 Distribution of inhalant allergy in the population of Lithuania Ruta Einikyte, Austeja Dapkeviciute, Jolita Norkuniene, Laima Skrickiene, Asta Miskiniene, Violeta Kvedariene Poster Session 2: Allergen molecules: identification, characterization, structure and function P13 Interference of antigen 5-based cross-reactivity in the diagnosis of hymenoptera venom allergy Maximilian Schiener, Bernadette Eberlein, Carmen Moreno-Aguilar, Gunilla Pietsch, Mareike Mc Intyre, Lea Schwarze, Dennis Rußkamp, Tilo Biedermann, Edzard Spillner, Ulf Darsow, Carsten Schmidt-Weber, Markus Ollert, Simon Blank P14 IgE cross-reactivity between European Hymenoptera and Asian hornet (Vespa velutina) venom allergens Cyril Longé, Andrea Brazdova, Jean-Louis Brunet, Claire Schwartz, Bruno Girodet, François Lavaud, Joelle Birnbaum, Nhân Pham Thi, Magalie Duchateau, Julia Chamot-Rooke, Laurence Guilloux, Marie-Ange Selva, Rémy Couderc, Hélène Sénéchal, Jean-Pierre Sutra, Pascal Poncet P15 Carbohydrate composition of house dust mite extracts and major group 1 and group 2 allergens Steffen Augustin, Linda Pump, Martin Wald, Thomas Eichhorn, Frank Fischer, Christoph Willers P16 Specificity of monoclonal antibodies against cross-reactive carbohydrate determinants Michaela Miehe, Melanie Plum, Sara Wolf, Frederic Jabs, Tim Raiber, Frank Bantleon, Henning Seismann, Thilo Jakob, Edzard Spillner P17 Red meat allergic patients have a selective IgE response to the a-Gal glycan Danijela Apostolovic, Anh Thu Tran, Sara Sanchez-Vidaurre, Tanja Cirkovic Velickovic, Maria Starkhammar, Carl Hamsten, Marianne Van Hage P18 Specificity of non-specific lipid transfer proteins and influence of the ligands on their three-dimensional structure Pawel Dubiela, Piotr Humeniuk, Sabine Pfeifer, Merima Bublin, Tomasz Borowski, Karin Hoffmann-Sommergruber P19 Real-time PCR analysis of Pru av 1 and Pru av 3 allergens Martie C.M. Verschuren, Shanna Bastiaan-Net, Defien Depoortere, Kay Foetisch, Stephan Scheurer, Harry J Wichers, Theo Noij P20 Specificity of anti-Pru av 1 antibodies for the detection of Pru av 1 isoallergens Martie C.M. Verschuren, Shanna Bastiaan-Net, Nikki M.E. Van Uden, Karel Vandenberghe, Kay Foetisch, Stephan Scheurer, Harry J. Wichers H.J., Theo H.M. Noij P21 Enhancing recombinant production yield of Bet v 1 through codon usage harmonization Anargyros Roulias, Maria Alejandra Parigiani, Heidi Hofer, Claudia Asam, Christof Ebner, Fátima Ferreira P22 Structural and dynamic insights into the world of PR-10 allergens Linda Ahammer, Sarina Grutsch, Martin Tollinger Poster Session 3: Allergen molecules: identification, characterization, structure and function P23 Purification of polcalcin from different pollen allergenic sources by antibody-affinity chromatography Raquel Moya, Mª Angeles López-Matas, Raquel Reyes, Jerónimo Carnés P24 Variations of wheat allergens in cultivars measured through a targeted quantitative mass spectrometry approach Colette Larré, Hélène Rogniaux, Roberta Lupi, Sandra Denery-Papini P25 Art v 1, Amb a 4 and Par h 1 defensin-like proteins share similar structural features but distinct immunological and allergenic properties Isabel Maria Pablos, Stephanie Eichhorn, Yoan Machado, Peter Briza, Christof Ebner, Jung-Won Park, Alain Didierlaurent, Naveen Arora, Stefan Vieths, Gabriele Gadermaier, Fatima Ferreira P26 Homogeneity or diversity of IgE-binding proteins in wheat dependant exercise induced anaphylaxis? Sandra Denery-Papini, Charlene Tanaka, Florence Pineau, Roberta Lupi, Martine Drouet, Etienne Beaudouin, Martine Morisset, Susan Altenbach P27 Deciphering the role of disulfide bonds and of repetitive epitopes in immunoglobulin E binding to wheat gliadins Sandra Denery-Papini, Hamza Mameri, Chantal Brossard, Roberta Lupi, Florence Pineau, Jean Charles Gaudin, Denise Anne Moneret-Vautrin, Etienne Beaudouin, Evelyne Paty, Martine Drouet, Olivier Tranquet, Colette Larré P28 Assessment of the allergenicity of soluble fractions from bread and durum wheats genotypes Roberta Lupi, Stefania Masci, Olivier Tranquet, Denise-Anne Moneret-Vautrin, Sandra Denery-Papini, Colette Larré P29 Isolation and characterization of Ara h 12 and Ara h 13: defensins, a novel class of peanut allergens Skadi Kull, Arnd Petersen, Marisa Böttger, Sandra Rennert, Wolf-Meinhard Becker, Susanne Krause, Martin Ernst, Thomas Gutsmann, Johann Bauer, Buko Lindner, Uta Jappe P30 Allergenicity attributes of different peanut market types Stef Koppelman, Shyamali Jayasena, Dion Luykx, Erik Schepens, Danijela Apostolovic, Govardus De Jong, Tom Isleib, Julie Nordlee, Joe Baumert, Steve Taylor, Soheila Maleki P31 The impact of peanut lipids on Ara h 1-induced immune responses in monocytes-derived dendritic cells Chiara Palladino, Barbara Gepp, Sofía Sirvent, Alba Angelina, Merima Bublin, Christian Radauer, Nina Lengger, Thomas Eiwegger, Oscar Palomares, Heimo Breiteneder P32 Compared allergenicity of native and thermally aggregated ovalbumin as large agglomerated particles Mathilde Claude, Roberta Lupi, Grégory Bouchaud, Marie Bodinier, Chantal Brossard, Sandra Denery-Papini P33 Simulation of the gastrointestinal digestion of the hazelnut allergens Cor a 9 and Cor a 11 by an in-vitro model and characterisation of peptidic products including epitopes by HPLC-MS/MS Robin Korte, Julia Bräcker, Jens Brockmeyer P34 Analysis of distribution of rice allergens in brown rice grain and allergenicity of the products containing rice bran Rie Satoh, Reiko Teshima Poster Session 4: Molecular approaches in AIT P35 Production of a recombinant hypoallergenic variant of the major peanut allergen Ara h 2 for allergen-specific immunotherapy Angelika Tscheppe, Dieter Palmberger, Merima Bublin, Christian Radauer, Chiara Palladino, Barbara Gepp, Nina Lengger, Reingard Grabherr, Heimo Breiteneder P36 Mutagenesis of amino acids critical for calcium-binding leads to the generation of a hypoallergenic Phl p 7 variant Marianne Raith, Linda Sonnleitner, Doris Zach, Konrad Woroszylo, Margit Focke-Tejkl, Herbert Wank, Thorsten Graf, Annette Kuehn, Ines Swoboda P37 Are birch pollen allergen immunotherapy induced blocking antibodies protective for cross-reactive allergens? Claudia Asam, Sara Huber, Heidi Hofer, Roland Lang, Thomas Hawranek, Fátima Ferreira, Michael Wallner P38 High success of 58 subcutaneous immunotherapy for pets allergy in a polyallergic cohort of patients: a component resolved individually adapted treatment (CRIAT) Fabienne Gay-Crosier P39 Neutrophils are potential antigen presenting cells in IgE- mediated allergy Dominika Polak, Birgit Nagl, Claudia Kitzmüller, Barbara Bohle P40 Characterization of allergen-specific CD8+ T cells in type I allergy Nazanin Samadi, Claudia Kitzmüller, Rene Geyeregger, Barbara Bohle, Beatrice Jahn-Schmid Poster Session 5: Molecular and cellular diagnostic tests P41 Nanofluidic-based biosensors allow quantification of total circulating IgE from a drop of blood in 5 minutes Aurélie Buchwalder, Ariel Gomez, Fabien Rebeaud, Iwan Märki P42 Allergen microarray for the analysis of serum IgE binding profile and allergenic activity Jaana Haka, Liisa Hattara, Marika Heikkinen, Merja H Niemi, Juha Rouvinen, Petri Saviranta, Pekka Mattila, Kristiina Takkinen, Marja-Leena Laukkanen P43 Generation of a well-characterized panel of periplaneta americana allergens for component resolved diagnosis Stephanie Eichhorn, Isabel Pablos, Bianca Kastner, Bettina Schweidler, Sabrina Wildner, Peter Briza, Jung-Won Park, Naveen Arora, Stefan Vieths, Gabriele Gadermaier, Fatima Ferreira P44 Improved diagnostic sensitivity of recombinant Api m 1 and Ves v 5 in diagnosis of Hymenoptera venom allergy Mira Silar, Julij Selb, Rok Kogovsek, Mitja Kosnik, Peter Korosec P45 Added value of biomarkers of primary sensitization and cross-reactivity in patients with hymenoptera venom allergy Leticia Pestana, Alcinda Campos Melo, Ana Mendes, Maria Elisa Pedro, Manuel Pereira Barbosa, Maria Conceição Pereira Santos P46 Cosensitization to Alt a 1 and Act d 2: more than a fortuitous association? Françoise Bienvenu, Claire Goursaud, Lorna Garnier, Sandrine Jacquenet, Michaël Degaud, Sébastien Viel, Annick Barre, Pierre Rougé, Jacques Bienvenu, Joana Vitte P47 Molecular diagnosis for peanut allergy: ALFA method performs as well as established methods for Ara h 1, Ara h 2, Ara h 6, Ara h 9 and CCD Amel Bensalah, Isabelle Cleach, Laurent Mousseau, Chantal Agabriel, Valérie Liabeuf, Joëlle Birnbaum, Jean-Louis Mège, Joana Vitte P48 Evaluation of a food challenge service in relation to specific IgE to molecular components in children with suspected peanut allergy James Gardner, Minal Gandhi, Harsha Kariyawasam, Giuseppina Rotiroti P49 Component resolved diagnosis in cereal allergy Isabel Carrapatoso, Celso Pereira, Frederico Regateiro, Emília Faria, Ana Todo-Bom Poster Session 6: Molecular diagnosis in prevention and therapy P50 Pretreatment molecular sensitizations determine the sIgG4 induction during the updosing of SCIT and may be useful to identify clinically relevant additional sensitizations Johannes Martin Schmid, Ronald Dahl, Hans Juergen Hoffmann P51 Usefulness of recombinant latex allergens in immunotherapy’s decision and follow-up Inês Mota, Filipe Benito Garcia, Angela Gaspar, Mário Morais-Almeida P52 Omega-5-gliadin in the diagnosis of wheat-dependent anaphylaxis induced by ibuprofen but not by exercise Joana Cosme, Letícia Pestana, Amélia Spínola Santos, Manuel Pereira Barbosa P53 Food dependent exercise-induced anaphylaxis: a component-resolved and in vitro depletion approach to access IgE cross-reactivity Diana Silva, Teresa Vieira, Ana Maria Pereira, André Moreira, Luís Delgado P54 Olive pollen allergens: what are we missing? Sara Prates, Cátia Alves, Elena Finelli, Paula Leiria Pinto P55 Purified Alt a 1 extract in Alternaria alternata allergy diagnosis Bárbara Kong Cardoso, Cíntia Cruz, Filipa Semedo, Elza Tomaz, Filipe Inácio P56 Use of specific IgE Bos d8 (casein) to aid early introduction of dietary baked milk in children with cows’ milk allergy James Gardner, Santanu Maity, Giuseppina Rotiroti, Minal Gandhi P57 Molecular characterisation and immunoreactivity of a peanut ingredient for use in oral food challenges Ivona Baricevic-Jones, Justin T. Marsh, Phil E. Johnson, Anuradha Balasundaram, Anya-May Hope, Aafke Taekema, Angela Simpson, Aida Semic-Jusufagic, E.N. Clare Mills P58 Specific IgE to recombinant allergens of hazelnut and oral food challenge in children Gourdon Dubois Nelly, Sellam Laetitia, Pereira Bruno, Michaud Elodie, Messaoudi Khaled, Evrard Bertrand, Fauquert Jean-Luc Poster session 7/8: miscellaneous P59 What defines a protein as an allergen? A discussion of sources and sufficiency Richard E. Goodman P60 Cat allergy: relationship between clinical and molecular diagnostic María Cecilia Martín Fernández De Basoa, Antón Fernández Ferreiro, Elena Rodríguez Plata P61 Anaphylaxis to rabbit: the cat came in last Luis Amaral, Borja Bartolomé, Alice Coimbra, Jose L Placido P62 Dog allergy: relationship between clinical and molecular diagnostic María Cecilia Martín Fernández De Basoa, Antón Fernández Ferreiro, Elena Rodríguez Plata P63 Correlation of serum timothy grass-pollen specific IgE levels determined by two immunoblot test systems Mariana Vieru, Florin-Dan Popescu, Florin-Adrian Secureanu, Carmen Saviana Ganea P64 Development of oral food challenge formulations for diagnosis of fish allergy using powdered fish ingredients Carol Ann Costello, Ivona Baricevic-Jones, Martin Sorensen, Clare Mills, Adrian Rogers, Aage Otherhals P65 Fish and peanut allergens interact with plasma membranes of intestinal and bronchial epithelial cells and induce differential gene expression of cytokines and chemokines Tanja Kalic, Isabella Ellinger, Chiara Palladino, Barbara Gepp, Eva Waltl, Verena Niederberger-Leppin, Heimo Breiteneder P66 Interleukin 4 affects fat tissue metabolism and expression of pro-inflammatory factors in isolated rat adipocytes Dawid Szczepankiewicz, Ewa Pruszynska-Oszmalek, Marek Skrzypski, Krzysztof W. Nowak, Aleksandra Szczepankiewicz P67 Ozone induced airway hyperreactivity in PD-L2−/− mice model Gwang-Cheon Jang P68 Thymic stromal lymphopoietin (TSLP) and its receptor as targets for the development of anti-inflammatory inhibitory agents Iva Markovic, Andreas Borowski, Tina Vetter, Andreas Wohlmann, Michael Kuepper, Karlheinz Friedrich P69 The mononuclear phagocyte system in experimentally-induced allergic rhinitis Ibon Eguiluz Gracia, Anthony Bosco, Ralph Dollner, Guro Reinholt Melum, Anya C Jones, Maria Lexberg, Patrick G Holt, Espen Sønderaal Bækkevold, Frode Lars Jahnsen P70 Expression of histamine metabolizing enzymes is increased in allergic children Aleksandra Szczepankiewicz, Paulina Sobkowiak, Marta Rachel, Beata Narozna, Dorota Jenerowicz, Witold Swiatowy, Anna Breborowicz P71 Modifying the glycosylation of human IgE towards oligomannosidic structures does not affect its biological activity Melanie Plum, Sara Wolf, Frank Bantleon, Henning Seismann, Frederic Jabs, Michaela Miehe, Thilo Jakob, Edzard Spillner P72 Flying Labs: an educational initiative to transfer allergy research into high-school settings Michael Wallner, Heidi Hofer, Fatima Ferreira, Reinhard Nestelbacher P73 Clinical significance of antihistamines and Kujin, an anti-allergic Kampo medicine Hiroyuki Fukui
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- 2016
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31. Applicability of epithelial models in protein permeability/transport studies and food allergy
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Cubells-Baeza, N., Verhoeckx, K.C.M., Larre, C., Denery-Papini, S., Gavrovic-Jankulovic, M., and Diaz Perales, A.
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- 2015
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32. Corrigendum: Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines(Front. Immunol., (2023), 13, (986823), 10.3389/fimmu.2022.986823)
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Pazos-Castro, Diego, Margain, Clémence, Gonzalez-Klein, Zulema, Amores-Borge, Marina, Yuste-Calvo, Carmen, Garrido-Arandia, Maria, Zurita, Lucía, Esteban, Vanesa, Tome-Amat, Jaime, Diaz-Perales, Araceli, and Ponz, Fernando
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Pru p 3 ,Urnip mosaic virus ,Antigen delivery ,Food allergy ,Plant biotechnology ,Immunotherapy ,Virus-like particles - Abstract
In the published article, there was an error in the author list, and author Marina Amores-Borge was erroneously excluded. The corrected author list appears below. “Diego Pazos-Castro, Clémence Margain, Zulema Gonzalez-Klein, Marina Amores- Borge, Carmen Yuste-Calvo, Maria Garrido-Arandia, Lucia Zurita, Vanesa Esteban, Jaime Tome-Amat, Araceli Diaz-Perales, Fernando Ponz”In the published article, there was an error. The missing author was not included in the Author Contributions section. A correction has been made to Author contributions. This sentence previously stated: “DP-C: Conceptualization, investigation, writing original draft. CM: Investigation. ZG-K: Conceptualization, investigation, writing review. CY-C: Investigation. MG-A: Investigation, writing review. LZ: Investigation. VE: Writing review, resources, funding acquisition. JT-A: Conceptualization, investigation, writing original draft. AD-P: Conceptualization, writing original draft, resources, funding acquisition. FP: Conceptualization, writing review,resources, funding acquisition. All authors contributed to the article and approved the submitted version.” The corrected sentence appears below: “DP-C: Conceptualization, investigation, writing original draft. CM: Investigation. ZG-K: Conceptualization, investigation, writing review. MA-B: Investigation. CY-C: Investigation. MG-A: Investigation, writing review. LZ: Investigation. VE: Writing review, resources, funding acquisition. JT-A: Conceptualization, investigation, writing original draft. AD-P: Conceptualization, writing original draft, resources, funding acquisition. FP: Conceptualization, writing review, resources, funding acquisition. All authors contributed to the article and approved the submitted version.” The authors apologize for these errors and state that they do not change the scientific conclusions of the article in any way. The original article has been updated.
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- 2023
33. NLRP3 priming due to skin damage precedes LTP allergic sensitization in a mouse model
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Diego Pazos-Castro, Zulema Gonzalez-Klein, Alma Yuste Montalvo, Guadalupe Hernandez-Ramirez, Alejandro Romero-Sahagun, Vanesa Esteban, Maria Garrido-Arandia, Jaime Tome-Amat, Araceli Diaz-Perales, Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Commission, Comunidad de Madrid, Universidad Politécnica de Madrid, Banco Santander, Pazos-Castro, Diego [0000-0002-0686-5131], Gonzalez-Klein, Zulema [0000-0003-3762-3781], Montalvo, Alma Yuste [0000-0002-4727-1284], Hernandez-Ramirez, Guadalupe [0000-0003-3031-6754], Romero-Sahagun, Alejandro [0000-0002-9417-8293], Garrido-Arandia, Maria [0000-0001-6114-5754], Tome-Amat, Jaime [0000-0003-4442-3649], Diaz-Perales, Araceli [0000-0002-1093-3627], Pazos-Castro, Diego, Gonzalez-Klein, Zulema, Montalvo, Alma Yuste, Hernandez-Ramirez, Guadalupe, Romero-Sahagun, Alejandro, Garrido-Arandia, Maria, Tome-Amat, Jaime, and Diaz-Perales, Araceli
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Sulfonamides ,Multidisciplinary ,Allergens ,Antigens, Plant ,Immunoglobulin E ,Immunity, Innate ,Disease Models, Animal ,Mice ,Indenes ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Lymphocytes ,Furans ,Food Hypersensitivity ,Plant Proteins - Abstract
Allergic sensitization is initiated by protein and epithelia interaction, although the molecular mechanisms leading this encounter toward an allergic phenotype remain unknown. Here, we apply the two-hit hypothesis of inflammatory diseases to the study of food allergy sensitization. First, we studied the effects of long-term depilation in mice by analyzing samples at different time points. Several weeks of depilation were needed until clear immunological changes were evidenced, starting with upregulation of NLRP3 protein levels, which was followed by overexpression of Il1b and Il18 transcripts. Secondly, we assessed the effects of allergen addition (in this case, Pru p 3 in complex with its natural lipid ligand) over depilated skin. Systemic sensitization was evaluated by intraperitoneal provocation with Pru p 3 and measure of body temperature. Anaphylaxis was achieved, but only in mice sensitized with Prup3_complex and not treated with the NLRP3 inhibitor MCC950, thus demonstrating the importance of both hits (depilation + allergen addition) in the consecution of the allergic phenotype. In addition, allergen encounter (but not depilation) promoted skin remodeling, as well as CD45+ infiltration not only in the sensitized area (the skin), but across several mucosal tissues (skin, lungs, and gut), furtherly validating the systemization of the response. Finally, a low-scale study with human ILC2s is reported, where we demonstrate that Prup3_complex can induce their phenotype switch (↑CD86, ↑S1P1) when cultured in vitro, although more data is needed to understand the implications of these changes in food allergy development., This research was funded by the Spanish Government (MINECO, grant BIO2017-84548- R); Instituto de Salud Carlos III (ISCIII) co-founded by FEDER Thematic Networks and Cooperative Research Centers: ARADYAL (RD16/0006/0003; RD16/0006/0013) and the Community of Madrid (FOODAL-CM_S2018/BAAA-4574). DPC was funded by Universidad Politécnica de Madrid and Banco Santander with a predoctoral Programa Propio grant. ARS was funded from the Community of Madrid included in the project FOOD-AL (FOODAL-CM_S2018/BAAA-4574). GHR was funded by European Comission (H2020-NMBP-X-KET-2017-768641—AllerScreening). JTA was funded from Instituto de Salud Carlos III (ISCIII) co-founded by FEDER Thematic Networks and Cooperative Research Centers: ARADYAL (RD16/0006/0003).
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- 2021
34. Suitability of potyviral recombinant virus-like particles bearing a complete food allergen for immunotherapy vaccines
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Pazos-Castro, Diego, primary, Margain, Clémence, additional, Gonzalez-Klein, Zulema, additional, Amores-Borge, Marina, additional, Yuste-Calvo, Carmen, additional, Garrido-Arandia, Maria, additional, Zurita, Lucía, additional, Esteban, Vanesa, additional, Tome-Amat, Jaime, additional, Diaz-Perales, Araceli, additional, and Ponz, Fernando, additional
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- 2022
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35. Distortion from planarity in arenes produced by internal rotation of one single hydroxyl hydrogen: The case of alternariol
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Garrido-Arandia, María, Gómez-Casado, Cristina, Díaz-Perales, Araceli, and Pacios, Luis F.
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- 2014
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36. Role of Art v 3 in pollinosis of patients allergic to Pru p 3
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Sánchez-López, Jaime, Tordesillas, Leticia, Pascal, Mariona, Muñoz-Cano, Rosa, Garrido, María, Rueda, Maria, Vilella, Ramón, Valero, Antonio, Díaz-Perales, Araceli, Picado, César, and Bartra, Joan
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- 2014
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37. Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages
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Hernandez-Ramirez, Guadalupe, primary, Pazos-Castro, Diego, additional, Gonzalez-Klein, Zulema, additional, Resuela-Gonzalez, Jose Luis, additional, Fernandez-Bravo, Sergio, additional, Palacio-Garcia, Lucia, additional, Esteban, Vanesa, additional, Garrido-Arandia, Maria, additional, Tome-Amat, Jaime, additional, and Diaz-Perales, Araceli, additional
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- 2022
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38. Lipid Ligands and Allergenic LTPs: Redefining the Paradigm of the Protein-Centered Vision in Allergy
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Gonzalez-Klein, Zulema, primary, Pazos-Castro, Diego, additional, Hernandez-Ramirez, Guadalupe, additional, Garrido-Arandia, Maria, additional, Diaz-Perales, Araceli, additional, and Tome-Amat, Jaime, additional
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- 2022
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39. Real‐life evaluation of molecular multiplex IgE test methods in the diagnosis of pollen associated food allergy
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Diem, Luisa, primary, Neuherz, Bellinda, additional, Rohrhofer, Johanna, additional, Koidl, Larissa, additional, Asero, Riccardo, additional, Brockow, Knut, additional, Diaz Perales, Araceli, additional, Faber, Margaretha, additional, Gebhardt, Julia, additional, Torres, María José, additional, Jensen‐Jarolim, Erika, additional, Zehetmayer, Sonja, additional, and Untersmayr, Eva, additional
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- 2022
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40. Modeling iron-catecholates binding to NGAL protein
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Gómez-Casado, Cristina, Roth-Walter, Franziska, Jensen-Jarolim, Erika, Díaz-Perales, Araceli, and Pacios, Luis F.
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- 2013
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41. Non‐specific lipid‐transfer proteins: Allergen structure and function, cross‐reactivity, sensitization, and epidemiology
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R Asero, Ronald van Ree, Olga Luengo, Joan Bartra, Karin Hoffmann-Sommergruber, Margaretha A. Faber, Lorenzo Cecchi, Stephen J. Till, Ines Swoboda, Elide A. Pastorello, Enrico Scala, Didier G. Ebo, Arazeli Diaz Perales, Domingo Barber, M. Fernandez-Rivas, Isabel Skypala, Francesca Gomez, Anastasios P Konstantinopoulos, Institut Català de la Salut, [Skypala IJ] Department of Allergy & Clinical Immunology, Royal Brompton & Harefield NHS Foundation Trust, Imperial College, London, UK. [Asero R] Ambulatorio di Allergologia, Clinica San Carlo, Milan, Italy. [Barber D] IMMA, School of Medicine, Universidad San Pablo CEU, CEU Universities, Madrid, Spain. RETIC ARADYAL RD16/0006/0015, Instituto de Salud Carlos III, Madrid, Spain. [Cecchi L] SOS Allergy and Clinical Immunology, USL Toscana Centro, Prato, Italy. [Diaz Perales A] Departamento de Biotecnología‐Biología Vegetal, Centro de Biotecnología y Genómica de Plantas (CBGP,UPM‐INIA),Universidad Politécnica de Madrid, Madrid, Spain. [Hoffmann-Sommergruber K] Department of Pathophysiology and Allergy Research, Medical University of Vienna, Austria. [Luengo O] Unitat d’Al·lèrgia, Servei de Medicina Interna, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. ARADyAL, Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus, and European Academy of Allergy, Clinical Immunology (EAACI) Task Force: Non-specific Lipid Transfer Protein Allergy Across Europe
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Pulmonary and Respiratory Medicine ,Ragweed ,Allergy ,Lipid transfer protein ,Epidemiology ,factores biológicos::antígenos::alérgenos [COMPUESTOS QUÍMICOS Y DROGAS] ,Immunology ,Otros calificadores::/diagnóstico [Otros calificadores] ,Biological Factors::Antigens::Allergens [CHEMICALS AND DRUGS] ,Review ,medicine.disease_cause ,Cross-reactivity ,Sensitization ,sensitization ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Allergen ,Mugwort ,Other subheadings::/diagnosis [Other subheadings] ,medicine ,Immunology and Allergy ,Artemisia vulgaris ,biology ,Immune System Diseases::Hypersensitivity::Hypersensitivity, Immediate::Food Hypersensitivity [DISEASES] ,business.industry ,Pharmacology. Therapy ,food ,Al·lèrgia alimentària - Diagnòstic ,lipid transfer protein ,RC581-607 ,biology.organism_classification ,medicine.disease ,allergy ,Anafilaxi ,medicine.anatomical_structure ,030228 respiratory system ,Food ,epidemiology ,Human medicine ,LTP ,Immunologic diseases. Allergy ,business ,Plant lipid transfer proteins ,enfermedades del sistema inmune::hipersensibilidad::hipersensibilidad inmediata::hipersensibilidad a los alimentos [ENFERMEDADES] - Abstract
Al·lèrgia; Epidemiologia; Proteïna de transferència de lípids Alergia; Epidemiología; Proteína de transferencia de lípidos Allergy; Epidemiology; Lipid transfer protein Background Discovered and described 40 years ago, non-specific lipid transfer proteins (nsLTP) are present in many plant species and play an important role protecting plants from stressors such as heat or drought. In the last 20 years, sensitization to nsLTP and consequent reactions to plant foods has become an increasing concern. Aim The aim of this paper is to review the evidence for the structure and function of nsLTP allergens, and cross-reactivity, sensitization, and epidemiology of nsLTP allergy. Materials and Methods A Task Force, supported by the European Academy of Allergy & Clinical Immunology (EAACI), reviewed current evidence and provide a signpost for future research. The search terms for this paper were “Non-specific Lipid Transfer Proteins”, “LTP syndrome”, “Pru p 3”, “plant food allergy”, “pollen-food syndrome”. Results Most nsLTP allergens have a highly conserved structure stabilised by 4-disulphide bridges. Studies on the peach nsLTP, Pru p 3, demonstrate that nsLTPs are very cross-reactive, with the four major IgE epitopes of Pru p 3 being shared by nsLTP from other botanically related fruits. These nsLTP allergens are to varying degrees resistant to heat and digestion, and sensitization may occur through the oral, inhaled or cutaneous routes. In some populations, Pru p 3 is the primary and sole sensitizing allergen, but many are poly-sensitised both to botanically un-related nsLTP in foods, and non-food sources of nsLTP such as Cannabis sativa, Platanus acerifolia, (plane tree), Ambrosia artemisiifolia (ragweed) and Artemisia vulgaris (mugwort). Initially, nsLTP sensitization appeared to be limited to Mediterranean countries, however more recent studies suggest clinically relevant sensitization occurs in North Atlantic regions and also countries in Northern Europe, with nsLTP sensitisation profiles being broadly similar. Discussion These robust allergens have the potential to sensitize and provoke symptoms to a large number of plant foods, including those which are raw, cooked or processed. It is unknown why some sensitized individuals develop clinical symptoms to foods whereas others do not, or indeed what other allergens besides Pru p 3 may be primary sensitising allergens. It is clear that these allergens are also relevant in non-Mediterranean populations and there needs to be more recognition of this. Conclusion Non-specific LTP allergens, present in a wide variety of plant foods and pollens, are structurally robust and so may be present in both raw and cooked foods. More studies are needed to understand routes of sensitization and the world-wide prevalence of clinical symptoms associated with sensitization to these complex allergens.
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- 2021
42. Supplementary data for the article: Costa, J.; Bavaro, S. L.; Benedé, S.; Diaz-Perales, A.; Bueno-Diaz, C.; Gelencser, E.; Klueber, J.; Larré, C.; Lozano-Ojalvo, D.; Lupi, R.; Mafra, I.; Mazzucchelli, G.; Molina, E.; Monaci, L.; Martín-Pedraza, L.; Piras, C.; Rodrigues, P. M.; Roncada, P.; Schrama, D.; Cirkovic-Velickovic, T.; Verhoeckx, K.; Villa, C.; Kuehn, A.; Hoffmann-Sommergruber, K.; Holzhauser, T. Are Physicochemical Properties Shaping the Allergenic Potency of Plant Allergens? Clinic Rev Allerg Immunol 2022, 62 (1), 37–63. https://doi.org/10.1007/s12016-020-08810-9.
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Costa, Joana, Bavaro, Simona Lucia, Benedé, Sara, Diaz-Perales, Araceli, Bueno-Diaz, Cristina, Gelencser, Eva, Klueber, Julia, Larré, Colette, Lozano-Ojalvo, Daniel, Lupi, Roberta, Mafra, Isabel, Mazzucchelli, Gabriel, Molina, Elena, Monaci, Linda, Martín-Pedraza, Laura, Piras, Cristian, Rodrigues, Pedro M., Roncada, Paola, Schrama, Denise, Ćirković-Veličković, Tanja, Verhoeckx, Kitty, Villa, Caterina, Kuehn, Annette, Hoffmann-Sommergruber, Karin, Holzhauser, Thomas, Costa, Joana, Bavaro, Simona Lucia, Benedé, Sara, Diaz-Perales, Araceli, Bueno-Diaz, Cristina, Gelencser, Eva, Klueber, Julia, Larré, Colette, Lozano-Ojalvo, Daniel, Lupi, Roberta, Mafra, Isabel, Mazzucchelli, Gabriel, Molina, Elena, Monaci, Linda, Martín-Pedraza, Laura, Piras, Cristian, Rodrigues, Pedro M., Roncada, Paola, Schrama, Denise, Ćirković-Veličković, Tanja, Verhoeckx, Kitty, Villa, Caterina, Kuehn, Annette, Hoffmann-Sommergruber, Karin, and Holzhauser, Thomas
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- 2022
43. Are Physicochemical Properties Shaping the Allergenic Potency of Animal Allergens?
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MS Dermatologie/Allergologie, Infection & Immunity, Lab Dermatologie/Allergologie, Costa, Joana, Villa, Caterina, Verhoeckx, Kitty, Cirkovic-Velickovic, Tanja, Schrama, Denise, Roncada, Paola, Rodrigues, Pedro M., Piras, Cristian, Martín-Pedraza, Laura, Monaci, Linda, Molina, Elena, Mazzucchelli, Gabriel, Mafra, Isabel, Lupi, Roberta, Lozano-Ojalvo, Daniel, Larré, Colette, Klueber, Julia, Gelencser, Eva, Bueno-Diaz, Cristina, Diaz-Perales, Araceli, Benedé, Sara, Bavaro, Simona Lucia, Kuehn, Annette, Hoffmann-Sommergruber, Karin, Holzhauser, Thomas, MS Dermatologie/Allergologie, Infection & Immunity, Lab Dermatologie/Allergologie, Costa, Joana, Villa, Caterina, Verhoeckx, Kitty, Cirkovic-Velickovic, Tanja, Schrama, Denise, Roncada, Paola, Rodrigues, Pedro M., Piras, Cristian, Martín-Pedraza, Laura, Monaci, Linda, Molina, Elena, Mazzucchelli, Gabriel, Mafra, Isabel, Lupi, Roberta, Lozano-Ojalvo, Daniel, Larré, Colette, Klueber, Julia, Gelencser, Eva, Bueno-Diaz, Cristina, Diaz-Perales, Araceli, Benedé, Sara, Bavaro, Simona Lucia, Kuehn, Annette, Hoffmann-Sommergruber, Karin, and Holzhauser, Thomas
- Published
- 2022
44. Are Physicochemical Properties Shaping the Allergenic Potency of Plant Allergens?
- Author
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Lab Dermatologie/Allergologie, Infection & Immunity, Costa, Joana, Bavaro, Simona Lucia, Benedé, Sara, Diaz-Perales, Araceli, Bueno-Diaz, Cristina, Gelencser, Eva, Klueber, Julia, Larré, Colette, Lozano-Ojalvo, Daniel, Lupi, Roberta, Mafra, Isabel, Mazzucchelli, Gabriel, Molina, Elena, Monaci, Linda, Martín-Pedraza, Laura, Piras, Cristian, Rodrigues, Pedro M., Roncada, Paola, Schrama, Denise, Cirkovic-Velickovic, Tanja, Verhoeckx, Kitty, Villa, Caterina, Kuehn, Annette, Hoffmann-Sommergruber, Karin, Holzhauser, Thomas, Lab Dermatologie/Allergologie, Infection & Immunity, Costa, Joana, Bavaro, Simona Lucia, Benedé, Sara, Diaz-Perales, Araceli, Bueno-Diaz, Cristina, Gelencser, Eva, Klueber, Julia, Larré, Colette, Lozano-Ojalvo, Daniel, Lupi, Roberta, Mafra, Isabel, Mazzucchelli, Gabriel, Molina, Elena, Monaci, Linda, Martín-Pedraza, Laura, Piras, Cristian, Rodrigues, Pedro M., Roncada, Paola, Schrama, Denise, Cirkovic-Velickovic, Tanja, Verhoeckx, Kitty, Villa, Caterina, Kuehn, Annette, Hoffmann-Sommergruber, Karin, and Holzhauser, Thomas
- Published
- 2022
45. Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients
- Author
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Gómez, Enrique, Díaz-Perales, Araceli, Tordesillas, Leticia, Doña, Inmaculada, Torres, María J., Blázquez, Ana B., Gómez, Francisca, Blanca, Miguel, and Mayorga, Cristobalina
- Published
- 2012
- Full Text
- View/download PDF
46. Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat
- Author
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Missaoui, Khawla, primary, Gonzalez-Klein, Zulema, additional, Jemli, Sonia, additional, Garrido-Arandia, Maria, additional, Diaz-Perales, Araceli, additional, Tome-Amat, Jaime, additional, and Brini, Faiçal, additional
- Published
- 2022
- Full Text
- View/download PDF
47. Expression and Interaction Analysis among Saffron ALDHs and Crocetin Dialdehyde
- Author
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Lourdes Gómez-Gómez, Luis F. Pacios, Araceli Diaz-Perales, María Garrido-Arandia, Javier Argandoña, Ángela Rubio-Moraga, and Oussama Ahrazem
- Subjects
saffron ,ALDHs ,crocetin ,structure modelling ,docking ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
In saffron, the cleavage of zeaxanthin by means of CCD2 generates crocetin dialdehyde, which is then converted by an unknown aldehyde dehydrogenase to crocetin. A proteome from saffron stigma was released recently and, based on the expression pattern and correlation analyses, five aldehyde dehydrogenases (ALDHs) were suggested as possible candidates to generate crocetin from crocetin dialdehydes. We selected four of the suggested ALDHs and analyzed their expression in different tissues, determined their activity over crocetin dialdehyde, and performed structure modeling and docking calculation to find their specificity. All the ALDHs were able to convert crocetin dialdehyde to crocetin, but two of them were stigma tissue-specific. Structure modeling and docking analyses revealed that, in all cases, there was a high coverage of residues in the models. All of them showed a very close conformation, indicated by the low root-mean-square deviation (RMSD) values of backbone atoms, which indicate a high similarity among them. However, low affinity between the enzymes and the crocetin dialdehyde were observed. Phylogenetic analysis and binding affinities calculations, including some ALDHs from Gardenia jasmonoides, Crocus sieberi, and Buddleja species that accumulate crocetin and Bixa orellana synthetizing the apocarotenoid bixin selected on their expression pattern matching with the accumulation of either crocins or bixin, pointed out that family 2 C4 members might be involved in the conversion of crocetin dialdehyde to crocetin with high specificity.
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- 2018
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48. Pollen and plant food profilin allergens show equivalent IgE reactivity
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Sirvent, Sofía, Tordesillas, Leticia, Villalba, Mayte, Díaz-Perales, Araceli, Cuesta-Herranz, Javier, Salcedo, Gabriel, and Rodríguez, Rosalía
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- 2011
- Full Text
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49. Plant non-specific lipid transfer proteins: An overview
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Missaoui, Khawla, primary, Gonzalez-Klein, Zulema, additional, Pazos-Castro, Diego, additional, Hernandez-Ramirez, Guadalupe, additional, Garrido-Arandia, Maria, additional, Brini, Faical, additional, Diaz-Perales, Araceli, additional, and Tome-Amat, Jaime, additional
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- 2022
- Full Text
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50. IgE-reactivity profiles to nonspecific lipid transfer proteins in a northwestern European country
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Faber, Margaretha A., Van Gasse, Athina L., Decuyper, Ine I., Uyttebroek, Astrid, Sabato, Vito, Hagendorens, Margo M., Bridts, Chris H., De Clerck, Luc S., Fernandez-Rivas, Montserrat, Pascal, Mariona, Diaz-Perales, Araceli, and Ebo, Didier G.
- Published
- 2017
- Full Text
- View/download PDF
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