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Xiaopei Xu, Qingnan Sun, Yang Liu, Dong Wang, Shuo Diao, Hanzhou Wang, Yuling Gao, Tianchao Lu, Junlin Zhou Department of Orthopedic Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People’s Republic of ChinaCorrespondence: Junlin Zhou, Department of Orthopedic Surgery, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, People’s Republic of China, Tel/Fax +86-01085231227, Email junlinzhou_article@outlook.comObjective: The aim was to evaluate the effects of different glenosphere eccentricities on impingement, range of motion (ROM), and muscle length during standard activities in reverse total shoulder arthroplasty (RSA).Methods: In this study, we utilized computational modeling techniques to create native shoulder and shoulder models undergoing RSA and simulate shoulder movements in all abduction-adduction, flexion-extension, and rotation. We tested a total of 36 different glenosphere configurations, which included three different inferior tilts (0°, +10°, +20°) and two different lateral offsets (0 mm and +4 mm), as well as six different glenosphere eccentricities (concentricity, inferior, posterior, anterior, anteroinferior, and posteroinferior). We evaluated the maximum impingement-free ROM, impingement sites, and muscle lengths.Results: All glenosphere configurations exceeded 50% of native shoulder ROM in three planes and total global ROM. In abduction-adduction, there was no significant difference among the different glenosphere eccentricities (p > 0.05). In flexion-extension, the posteroinferior eccentricity had the maximum ROM among the different eccentricities, but no significant difference among the different glenosphere eccentricities (p > 0.05). In rotation, there was a significant difference overall, and anteroinferior eccentricity had a significant advantage over concentricity (p < 0.05). In total global ROM, anteroinferior eccentricity had a significant advantage over concentricity when lateral offset was 0 mm (p < 0.05). In all models of glenosphere eccentricities, only the elongation of the infraspinatus muscle was statistically significant (p < 0.05).Conclusion: Glenosphere eccentricity significantly influenced rotation, total global ROM, and the length of the subscapularis muscle. Among them, anteroinferior offset achieved the maximum ROM in abduction-adduction, rotation, and total global activities. Both anteroinferior and inferior glenoid eccentricity showed significant advantages over the concentricity in rotation and total global ROM.Level of Evidence: Basic Science Study; Computer Modeling.Graphical Abstract: Keywords: reverse shoulder arthroplasty, glenosphere eccentricities, impingement, notching, range of motion, muscle length

Xiaopei Xu,&ast; Hanzhou Wang,&ast; Yang Liu, Dong Wang, Shuo Diao, Yuling Gao, Junlin Zhou Department of Orthopedic Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Junlin Zhou, Department of Orthopedic Surgery, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, People’s Republic of China, Tel/Fax +86-1085231227, Email junlinzhou_article@outlook.comObjective: The aim of this study is to evaluate the diagnostic accuracy of infection-related biomarkers in high-risk lower limb injury patients with fracture-related infection (FRI) caused by high-/low-virulence microorganisms.Methods: This study was a retrospective analysis of patients with high-risk lower extremity fractures (including tibial plateau, calcaneus, and Pilon fractures) who underwent open reduction internal fixation (ORIF) surgery from January 2017 to February 2022. Peripheral blood samples were collected within 24 hours of admission, and the following information was evaluated: gender, age, BMI, smoking, comorbidities, injury information, surgical details, values for serum C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), as well as neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR).Results: A total of 576 patients receiving lower extremity fracture surgery were included in this study. Fifty-one patients (8.85%) were identified as FRI, and 28 (54.9%) of these 51 cases were further classified as high-virulence group. The median levels of CRP, ESR, NLR, and MLR were significantly higher in the FRI group than in the non-FRI group (p < 0.01). Similarly, the marginally significantly higher levels of CRP and NLR presented in the high-virulence group, compared to the low-virulence group (p < 0.1). The AUC areas of CRP, NLR, and CRP+NLR were 0.826, 0.650, and 0.873, respectively. We calculated the optimal cut-off points for CRP+NLR as diagnostic markers of high-virulent infection was 0.377.Conclusion: This study showed the incidence of FRI in high-risk lower extremity fractures was 8.9%, and identified preoperative serum biomarkers, including CRP, ESR, NLR, and PLR, as useful tools for assisting in the diagnosis of infection. Additionally, the combination of CRP with NLR played a discriminating clinical role in postoperative infections caused by different virulence.Level of Evidence: Clinical study.Keywords: high-risk lower extremity fractures, fracture-related infection, high- / low-virulence, serum inflammatory biomarkers, receiver operating characteristic

Dong Wang, Yang Liu, Shuo Diao, Lei Shan, Junlin Zhou Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People’s Republic of ChinaCorrespondence: Junlin Zhou, Department of Orthopedics, Beijing Chaoyang hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, People’s Republic of China, Tel +86-13240718193, Email junlinzhou_article@outlook.comPurpose: To investigate the effect of macrophage exosomal long non-coding (lnc)RNAs on bone mesenchymal stem cell (BMSC) osteogenesis and the associated mechanism.Methods: Rat BMSCs and spleen macrophages were co-cultured with serum derived from the fracture microenvironment of rat tibia. BMSC osteogenesis was evaluated using Alizarin red staining and the expression of BMP-2, RUNX2, OPN, and OC mRNA. BMSC osteogenesis was evaluated after co-culture with macrophages stimulated using hypoxic conditions or colony-stimulating factor (CSF). The uptake of macrophage-derived exosomes by BMSCs was evaluated using the exosome uptake assay. High-throughput sequencing and bioinformatics analyses were performed to identify key lncRNAs in the macrophage exosomes. The effect of lncRNA expression levels on BMSC osteogenesis was also assessed using a lncRNA overexpression plasmid and siRNA technology. M1 and M2 macrophages were distinguished using flow cytometry and the key exosomal lncRNA was detected by in situ hybridization.Results: In the fracture microenvironment, macrophages (stimulated using either hypoxia or CSF) significantly increased the osteogenic ability of BMSCs. We showed that BMSCs assimilated macrophage-derived vesicles and that the inhibition of exosomal secretion significantly attenuated the macrophage-mediated induction of BMSC osteogenesis. The hypoxia condition led to the up-regulation of 310 lncRNAs and the down-regulation of 575 lncRNAs in macrophage exosomes, while CSF stimulation caused the up-regulation of 557 lncRNAs and the down-regulation of 407 lncRNAs. In total, 108 lncRNAs were co-up-regulated and 326 lncRNAs were co-down-regulated under both conditions. We eventually identified LOC103691165 as a key lncRNA that promoted BMSC osteogenesis and was expressed at similar levels in both M1 and M2 macrophages.Conclusion: In the fracture microenvironment, M1 and M2 macrophages promoted BMSC osteogenesis by secreting exosomes containing LOC103691165.Keywords: macrophage exosomes, BMSCs osteogenesis, lncRNAs, LOC103691165, bone fracture microenvironment

Bacterial infections are common during induction therapy in children and adolescents with acute leukaemia and may cause infection-related mortality. To determine the efficacy and safety of prophylactic antibiotics in paediatric patients with acute leukaemia receiving induction chemotherapy. From three English databases and four Chinese databases, we searched for randomized controlled trials (RCTs) and cohort studies that compared prophylactic antibiotics to placebo, no prophylaxis, or that compared one antibiotic versus another in paediatric patients with acute leukaemia undergoing induction chemotherapy. Two reviewers independently screened the studies, extracted data, and assessed the risk of bias using Cochrane Risk of Bias 2 tool and Newcastle–Ottawa Scale, and the certainty of evidence using Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Two RCTs and ten cohort studies were finally included. For children with acute lymphoblastic leukaemia, antibiotic prophylaxis, including levofloxacin, sulfamethoxazole–trimethoprim, or other antibiotics, probably reduced bacteraemia (risk ratio (RR): 0.44; 95% confidence interval (CI): 0.33–0.60; moderate certainty) without significantly increasing Clostridioides difficile infection (CDI) or invasive fungal infection. Levofloxacin reduced the CDI rate (RR: 0.08; 95% CI: 0.01–0.62; high certainty). Ciprofloxacin prophylaxis probably reduced infection-related mortality (RR: 0.12; 95% CI: 0.01–0.97; moderate certainty). In children with acute myeloid leukaemia, ciprofloxacin plus vancomycin may reduce febrile neutropenia (RR: 0.79; 95% CI: 0.66–0.94; low certainty). Individual studies indicated that prophylaxis increased antibiotic exposure but reduced non-preventive antibiotic exposure. In children with acute leukaemia undergoing induction therapy, antibiotic prophylaxis may improve the bacterial infection and mortality. [ABSTRACT FROM AUTHOR]

Shi-Tong Diao, Run Dong, Jin-Min Peng, Yan Chen, Shan Li, Shu-Hua He, Yi-Fan Wang, Bin Du, Li Weng On behalf of the China Critical Care Clinical Trials Group (CCCCTG)Medical Intensive Care Unit, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, People’s Republic of ChinaCorrespondence: Li Weng, Medical Intensive Care Unit, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, 1 Shuai Fu Yuan, Beijing, 100730, People’s Republic of China, Email wengli@gmail.comBackground: Sepsis surveillance was important for resources allocation, prevention, and development of health policy.Objective: The aim of the study was to validate a modified International Classification of Diseases (ICD)-10 based algorithm for identifying hospitalized patients with sepsis.Methods: We retrospectively analyzed a prospective, single-center cohort of adult patients who were consecutively admitted to one medical ICU ward and ten non-ICU wards with suspected or confirmed infections during a 6-month period. A modified ICD-10 based algorithm was validated against a reference standard of Sequential Organ Failure Assessment (SOFA) score based on Sepsis-3. Sensitivity (SE), specificity (SP), positive predictive value (PPV), negative predictive value (NPV), and areas under the receiver operating characteristic curves (AUROCs) were calculated for modified ICD-10 criteria, eSOFA criteria, Martin’s criteria, and Angus’s criteria.Results: Of the 547 patients in the cohort, 332 (61%) patients met Sepsis-3 criteria and 274 (50%) met modified ICD-10 criteria. In the ICU setting, modified ICD-10 criteria had SE (84.47%), SP (88.57%), PPV (95.60), and NPV (65.96). In non-ICU settings, modified ICD-10 had SE (64.19%), SP (80.00%), PPV (80.33), and NPV (63.72). In the whole cohort, the AUROCs of modified ICD-10 criteria, eSOFA, Angus’s criteria, and Martin’s criteria were 0.76, 0.75, 0.62, and 0.62, respectively.Conclusion: This study demonstrated that modified ICD-10 criteria had higher validity compared with Angus’s criteria and Martin’s criteria. Validity of the modified ICD-10 criteria was similar to eSOFA criteria. Modified ICD-10 algorithm can be used to provide an accurate estimate of population-based sepsis burden of China.Keywords: sepsis, validation, International Classification of Diseases, administrative health data, identification

Objective To investigate the clinical and imaging features of the characteristic "heart appearance" isolated cerebral infarction involving bilateral pons-medulla oblongata. Methods The clinical and imaging data of 2 patients with bilateral pons-medulla oblongata "heart appearance" isolated infarction treated in our hospital from May 2019 to February 2020 were retrospectively analyzed. The published domestic and foreign literature of patients with this disease collected in PubMed, Web of Science, CNKI, WanFang and other databases in recent 10 years were also searched by computer, and the literature was subsequently reviewed and analyzed. Results The course of the disease in both patients was progressive, with clinical manifestations of bulbar palsy, sensory disturbance, progressive quadriplegia, and respiratory involvement. Brain magnetic resonance imaging (MRI) showed a typical "heart appearance" isolated infarction in the medial medulla or pons. Digital subtraction angiography (DSA) revealed vertebrobasilar atherosclerosis in case 1 and vertebrobasilar atherosclerosis, stenosis, and occlusion in case 2. A total of 29 patients were contained in 24 pieces of literature, including 21 cases of medulla oblongata infarction and 8 cases of pontine infarction, all with "heart appearance". Thereby a total of 31 patients, including 28 males, and 3 females, were reported by our center and the literature, with onset age of 32~87 (60.3±12.1) years. Among them, there were 29 cases of bulbar palsy symptoms (29/31, 93.55%), 23 of sensory impairment (23/31, 74.19%), 31 of quadriplegia (100%), 29 with progressive disease course (29/31, 93.55%), 23 with respiratory involvement (23/31, 74.19%), 24 with poor prognosis (24/29, 82.76%), and death in 6 cases (6/29, 20.69%). Of the 29 patients examined by MRA, CTA or DSA, 7 had no obvious abnormality or only atherosclerosis in the vertebrobasilar artery (7/29, 24.14%), 11 had vertebrobasilar stenosis or dysplasia (11/29, 37.93%), and the remaining 11 showed vertebrobasilar artery occlusion (11/29, 37.93%). Conclusion Isolated "heart appearance" infarction of the bilateral medial pons-medulla oblongata is clinically rare and has special morphological features on brain MRI. The etiology of such patients is mainly vertebrobasilar atherosclerosis, stenosis or occlusion. The course of the disease is often progressive, quadriplegia, and involves breathing, with critical condition, which is prone to misdiagnosis and missed diagnosis. Brain MRI and DSA examination are suggested to be performed as soon as possible to facilitate early diagnosis and treatment to improve the prognosis.

The soil organic matter (SOM) and soil total nitrogen (STN) is a significant concern in Qinghai–Tibet Plateau, China. This study analysed the spatial distribution of SOM and STN and determined their influencing factors to support the conservation of cultivated soil and development of sustainable agricultural strategies in the Plateau. In total, 120 soil samples were collected from the 0–20-cm soil layer in Huzhu County, Qinghai Province. Traditional statistical and geostatistical methods were used to analyse the spatial distribution of SOM and STN; a geographical detector (GeoDetector)was used to explore the factors influencing the spatial variation. The SOM and STN concentrations were 6.92–44.57 and 0.52–2.54 g/kg, respectively. The Cokriging interpolation map showed a similar spatial distribution pattern for SOM and STN concentrations, which decreased from the northeast to southwest directions in the study area. GeoDetector results revealed that the three primary factors influencing the spatial variability of SOM and STN were soil type, annual accumulated precipitation and elevation, with their explanatory power ranging between 38.4% and 59.5%. Two-factor interactions enhanced the explanatory power of the spatial variability of SOM and STN. The research results provide a reference for conservation tillage and precision agriculture.

Copyright of Bulletin de la Société de Pathologie Exotique is the property of John Libbey Eurotext Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bulletin de la Société de Pathologie Exotique is the property of John Libbey Eurotext Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Assessing the risk and sources of heavy metals in cultivated soil is important for the agricultural development of the Qinghai–Tibet Plateau. A survey was conducted in this study to determine the spatial distribution and possible sources of heavy metals in the agricultural soils in Huzhu county, a representative agricultural suburb in Qinghai-Tibet Plateau. A total of 120 surface soil samples were collected, soil pH and concentrations of chromium(Cr), cadmium(Cd), plumbum(Pb), arsenic(As), and hydrargyrum(Hg) were analyzed. The pollution risk degree of heavy metal pollution was also evaluated. The mean concentrations of the heavy metals were 70.8, 0.24, 23.7, 12.2, and 0.08 mg/kg for Cr, Cd, Pb, As, and Hg, respectively, higher than the soil background values of Qinghai Province with the exception of As, but lower than the guideline values of Chinese standard for soil contamination of agricultural land. The sampling point contamination risk of the heavy metals showed a site feature, and most sampling points of Cd and Hg was exhibit polluted. The potential ecological risk index indicated that most of the cultivated soils have low and moderate ecological risks. Multivariate and geostatistical analysis suggested that Cr, As, and Pb were due mainly to soil parent materials, Cd contamination was caused by agricultural and transportation activities, and Hg contamination was caused by coal combustion and industrial activities. The results of this study is a basis for undertaking more effective and accurate heavy metal control strategies to protect the safety of agricultural ecosystems.

Cellulose graphene composite aerogels (CMC/GA) were prepared by one-step hydrothermal reaction based on graphite oxidation with the addition of low cost carboxymethyl cellulose. The functional group structure and microstructure of CMC/GA were characterized. The mechanism and capacity of the adsorption of Methylene Blue (MB) were investigated by CMC/GA in water. The results show that the higher temperature and higher initial concentration of MB are beneficial to the adsorption of CMC/GA. The adsorption isotherm is fitted with Langmuir model, the adsorption activation energy of the system is 57.951 kJ·mol-1, indicating that the adsorption of MB by CMC/GA is monolayer and belongs to chemical adsorption. The adsorption kinetics of MB is fitted with pseudo-second-order kinetics model. The intrapartical diffusion model of MB with different concentration shows that the adsorption process of CMC/GA for MB can be divided into two stages:macroporous diffusion and microporous diffusion, and the diffusion rate of macroporous diffusion is obviously higher than that of microporous diffusion.

It is necessary to develop novel nanocomposite films for reducing soil pollution caused by the use of petroleum-based materials. In this paper, a simple solvent-cast method was used to fabricate gutta percha films with carbon nanotubes (CNTs) by homogeneously dispersing different amount of CNTs (0%, 0.5%, 1%, 1.5% and 2%, wt/%) through gutta percha matrix. The obtained nanocomposite films were characterized by Raman spectroscopy, atomic force microscopy (AFM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Meanwhile, the wettability, mechanical properties, water vapor barrier properties, electromagnetic absorption of films as well as their photo-thermal conversion of the soil were investigated in detail. The results showed that the 1% loading of CNTs film significantly improved the mechanical properties of the nanocomposite films compared to the control: the tensile strength, elongation at break and Young's modulus were increased by 44.0%, 30.5% and 26.1%, respectively. In addition, the CNTs/gutta percha films demonstrated lower thermal stability, glass transition temperature (Tg), melting enthalpy (ΔHm) crystallinity and water vapor permeability (WVP) than the pristine gutta percha film. Especially, the WVP value of the film with 1% CNTs was the lowest. And finally, the soil warming experiments showed that the 1% CNTs film had the highest 83.73% photo-thermal conversion efficiency, compared to the 18.61% for control. This nanocomposite film has the potential for application in agriculture in arid regions because of its high stretchability and photo-thermal conversion efficiency, which could be potential application in agricultural mulch.

An efficient source of all-solid-state broadly tunable mid-infrared optical parametric oscillator based on a periodically poled MgO-doped lithium niobate is reported. The pump source is a 1064nm acousto-optically Q-switched diode-pumped Nd:YAG laser. A broadly tunable mid-infrared output from 1.56 to 1.67 µm were generated, with corresponding idler wavelengths of 3.34 to 2.93 µm by temperature tuning from 40 to 200°C. When the average pump power is 1.61 W with about 70 ns pulse duration operating at a repetition rate of 10 kHz, the maximum signal output power of the PPMgLN-OPO is about 211 mW at 1631 nm. [ABSTRACT FROM AUTHOR]

Under inventory financing mode, port offers joint logistics and financial services to a capital-constrained cargo-owner whose cargos are taken as collaterals. Port takes financial risk while gaining profit from both traditional logistics and derived financial services. This paper adopts Mean-CVaR criterion to formulate objective function and derive the equilibrium loan-to-value ratio. The Mean-CVaR model proposed in this article depicts risk-aversion attitude and decision preference of port under the uncertainty of collateral’s demand. The result shows that there is a positive relation between decision preference and loan-to-value ratio. The growth in risk aversion attitude prompts port to act cautiously by reducing the loan-to-value ratio.

Major depressive disorder is a common emotional mood disorder disease, which mainly manifests as depression, cognitive impairment, weakened volition activities, loss of appetite, and sleep disorders. Depression is a common mental disorder affecting more than 264 million people worldwide. There are many antidepressant drugs in clinic, e.g., tricyclic antidepressants, monoamine oxidase inhibitors, selective serotonin (5-HT) reuptake inhibitors and selective norepinephrine (NA) reuptake inhibitors. Although great progress has been made in the research of new antidepressant drugs, the current clinical use of drugs still has many shortcomings, e.g., the unmet efficiency, slow onset of action, and multiple adverse reactions, which are difficult to meet the requirements of the increasing number of the depressed patients. This article mainly focuses on the development process of antidepressant drugs and compares their effects. Specifically, the N-methyl-D-aspartate receptor as a new antidepressant has a good effect, which is worthy of further study. These results shed light for providing a research idea for the study of new antidepressants.

Electrosorption is a novel desalination technique that has many advantages in the treatment of sewage. However, commercially available activated carbon electrodes for electrosorption commonly have low microporosity, poor moulding performance, and low adsorption and regeneration efficiency. Here, we evaluated a novel adsorbent material, activated carbon fibre felt (ACFF), for electrosorption of chromium ions (Cr6+) in sewage treatment. The ACFF was modified with 20% nitric acid and its modified structure was characterized. The modified ACFF was used as an adsorbing electrode to investigate its desalination effect by electrosorption. Results showed that compared with those of unmodified ACFF, the modified ACFF had more carbonyl and carboxyl groups and the specific surface area, average pore size and micropore volume of the modified ACFF also improved by 32.2%, 2.5% and 23.1%, respectively. The kinetics of Cr6+ adsorption conformed to the pseudo-second-order kinetic equation, and the adsorption isotherm conformed to the Langmuir model. In addition, the regeneration rate of the modified ACFF electrode was more than 94%. In conclusion, the modified ACFF exhibits excellent electrosorption and regeneration performance for Cr6+ removal from water and thus is of great value for promotion in sewage treatment.

The twenty-first century is a new century in ocean development and utilization. With increasing attention paid by the government, geological marine surveys, in particular geological marine analysis and testing technology has gained significant progress. The latest progresses on China′s geological marine analysis and testing technology is briefly summarized in this paper. Progress is listed as follows: establishing a fast and efficient systematic method for simultaneous multi-element analysis of geological marine samples by large analytical instruments, in order to meet the demand of regional geological marine surveys and geological coastal surveys; establishing and expanding test technology of anomaly identification and application for gas hydrate samples，including the methods of rapid qualitative tests on anions (Cl-, Br-, SO42-) and cations (Na+, NH4+, K+, Ca2+, Mg2+) in pore water by ion chromatography, and new detection technologies on natural gas hydrate simulated experiments based on ultrasonic, resistance and time domain reflectometry (TDR). Establishing an integrated method for organic pollutant samples in marine sediments to simultaneously purify, separate and test, which mainly applies a composite SPE column to separate and uses C6H14 and C6H14/CH2Cl2 solution to leach, so as to effectively retain the substrate impurity and thus significantly improve the test efficiency is also summarized. In addition, other progresses are also introduced, such as ocean mineral sample analysis technology, geological marine reference material development and ship-carried and in-situ chemical detection technology. It is speculated that the ship-carried and in-situ testing technology in the marine investigation and monitoring will become more and more important, and that strengthening the performance of marine chemical sensors and detection of integrated technology is an important development direction in the future.

So far, gas hydrates not only have been explored in marine and frozen areas of China, but also have been sampled successfully. The thermal conductivity of sediments with gas hydrates is a key parameter for estimating the resource and designing a proper exploitation plan. Restricted to the stable conditions for hydrates and their measurement technique, much more work need to be done on the thermal conductivity investigation of hydrates. In this paper, a description of the measurement technique of the thermal conductivity and saturation of gas hydrates formed in marine sediment is given. Marine sediment collected from the Shenhu area of the South China Sea was taken as the porous media. The methane hydrates in them were synthesized under the conditions of 7.8 MPa pressure and 2℃ temperature. The thermal conductivity and saturation were measured by thermal pulse probe technique coupled with time domain reflection method. Based on the results, when the hydrate saturation changed from 0 to 49%, the thermal conductivity appeared to be initially increase and then decrease. It was deduced that the relationship between thermal conductivity and saturation was controlled by the hydrate filling pattern. In this experiment, gas hydrates priority selection in particle void nucleation and growth, and gradually cement with sediments.