1. Comparison of the Idylla™ Respiratory (IFV-RSV) panel with the GeneXpert Xpert® Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples
- Author
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Diana Koletzki, W. Laffut, Marc Van Ranst, Els Rousseau, Steven Vissers, Yannick Wouters, Theresa Pattery, Ellen Van Even, Els Keyaerts, and Annabel Rector
- Subjects
0301 basic medicine ,Microbiology (medical) ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,030106 microbiology ,Respiratory Syncytial Virus Infections ,Virus ,Specimen Handling ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Internal medicine ,Nasopharynx ,Influenza, Human ,medicine ,Sample Type ,Humans ,Sampling (medicine) ,030212 general & internal medicine ,Respiratory system ,Child ,Reference standards ,Aged ,Retrospective Studies ,Aged, 80 and over ,GeneXpert MTB/RIF ,business.industry ,Infant, Newborn ,Infant ,Retrospective cohort study ,Influenza a ,General Medicine ,Middle Aged ,Orthomyxoviridae ,Infectious Diseases ,Molecular Diagnostic Techniques ,Child, Preschool ,Respiratory Syncytial Virus, Human ,Female ,Nasal Cavity ,business - Abstract
The objective of this study was to compare the performance of the Idylla™ Respiratory (IFV-RSV) panel to the GeneXpert Xpert® Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert® assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla™ assay showed excellent performance compared to the GeneXpert® assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab.
- Published
- 2018