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1. Fluorescent polarization assay, two versions of indirect and competitive enzyme- linked immunoassay for brucellosis diagnosis in vaccinated buffaloes (Bubalus bubalis)

Author

Diana Elina Martinez, Nerina Aguirre, María Fabiana Cipolini Galarza, and Susana Marta Torioni de Echaide

Subjects
brucellosis, buffaloes, serological diagnosis, Agriculture, Agriculture (General), S1-972
Abstract

ABSTRACT: The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent assay (I-ELISA) and the competitive enzyme-linked immunosorbent assay (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. The V1 of I-ELISA identifies them through the monoclonal (M23) anti-bovine IgG (I-ELISAM23) and the V2 through the ProteinA / G (I-ELISA-A/G). Serum samples of 862 buffaloes (Bubalus bubalis) from the Northeast of Argentina (NEA) were analyzed using the complement fixation test (CFT) as the reference. Receiving Operator Characteristic (ROC) analysis defined for the area under the curve (AUC) determined the cutoff points, sensitivity (Se) and specificity (Sp) for each test. CFT identified 107 positive and 755 negative sera. The best AUC (0.986), Concordance with CFT (96.3%) and kappa value (0.843) was obtained by I-ELISA A/G test. This assay showed the highest Se (95.33%) and C-ELISA the highest Sp (97%). FPA failed to measure the antibodies in 23 (2.65%) serum samples due to unsuccessful reading. I-ELISA M23 proved to be ineffective to diagnose brucellosis in bubaline sera. The four serological tests showed cutoff points lower than those standardized for bovines. As conclusion, I-ELISA A/G, C-ELISA and FPA with its limitations would be effective techniques for the diagnosis of brucellosis in buffaloes in the NEA, requiring an appropriate cut-off point to guarantee their maximum performance in this species.

Published
2021
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2. ESTUDIO PROSPECTIVO DE LAS PRIMO INFECCIONES POR BABESIA BOVIS EN TERNEROS BRAHMAN Y BRANGUS DE UN ÁREA ENZOÓTICA DE CORRIENTES, ARGENTINA

Author

I.E. Echaide, I. Martínez, R. Jacobo, F. Cipolini, C. Storani, A. Ragazzi, S. Torioni de Echaide, and Diana Elina Martinez

Subjects
Breeds (animals), Raza Brahman, Raza Brangus, Babesiosis, Ganado Bovino, Babesia bovis, Cattle, Corrientes, Calves, Enfermedades de los Animales, Razas (animales), Ternero, Animal Diseases
Abstract

Se evaluaron primo-infecciones causadas por Babesia bovis en terneros Brahman y Brangus en zona endémica de Corrientes, mediante la detección de: anticuerpos por ELISA-i, hematocrito y parasitemia en 48 vacas gestantes (195 días), de sus crías dentro de las 6 horas de vida (M1) y periódicamente hasta los 10 meses de edad (M2-M9). Para determinar la ingestión de calostro se utilizó la prueba del glutaraldehído (M1). La prevalencia de B. bovis en vacas fue del 97,91%. Los niveles de anticuerpos (M1) en terneros calostrados (n=15) fue 79,4 (14/15) Porcentaje de Positividad (PP), y el de sus madres 57,5% (47/48) PP (p

Published
2015
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3. Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas

Author

Carolina S. Thompson, Vilma Canavesio, Diana Elina Martinez, Patricia Zimmer, Roberto Jacobo, Ana M. Nicola, Sebastian Elena, Graciela Draghi, and Susana Torioni de Echaide

Subjects
Buffaloes, Genotype, Biovar, Argentina, Brucella abortus, Brucella, Polymerase Chain Reaction, Microbiology, Brucellosis, Fetus, medicine, Animals, Typing, Insertion sequence, Genotyping, General Veterinary, biology, Immune Sera, General Medicine, biology.organism_classification, medicine.disease, Virology, Bacterial Typing Techniques, Phenotype, Bubalus
Abstract

An isolate of Brucella spp. from an aborted water buffalo ( Bubalus bubalis ) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab , and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus – B. melitensis – B. ovis – B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis . Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas.

Published
2014
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