1,352 results on '"Di Silvestre, A"'
Search Results
2. Arabidopsis thaliana Early Foliar Proteome Response to Root Exposure to the Rhizobacterium Pseudomonas simiae WCS417
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Francesca Marzorati, Rossana Rossi, Letizia Bernardo, Pierluigi Mauri, Dario Di Silvestre, Emmanuelle Lauber, Laurent D. Noël, Irene Murgia, and Piero Morandini
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Arabidopsis thaliana ,formate dehydrogenase (FDH) ,glutathione S-transferase (GST) ,hydathodes ,proteome ,Pseudomonas simiae WCS417 ,Microbiology ,QR1-502 ,Botany ,QK1-989 - Abstract
Pseudomonas simiae WCS417 is a plant growth–promoting rhizobacterium that improves plant health and development. In this study, we investigate the early leaf responses of Arabidopsis thaliana to WCS417 exposure and the possible involvement of formate dehydrogenase (FDH) in such responses. In vitro–grown A. thaliana seedlings expressing an FDH::GUS reporter show a significant increase in FDH promoter activity in their roots and shoots after 7 days of indirect exposure (without contact) to WCS417. After root exposure to WCS417, the leaves of FDH::GUS plants grown in the soil also show an increased FDH promoter activity in hydathodes. To elucidate early foliar responses to WCS417 as well as FDH involvement, the roots of A. thaliana wild-type Col and atfdh1-5 knock-out mutant plants grown in soil were exposed to WCS417, and proteins from rosette leaves were subjected to proteomic analysis. The results reveal that chloroplasts, in particular several components of the photosystems PSI and PSII, as well as members of the glutathione S-transferase family, are among the early targets of the metabolic changes induced by WCS417. Taken together, the alterations in the foliar proteome, as observed in the atfdh1-5 mutant, especially after exposure to WCS417 and involving stress-responsive genes, suggest that FDH is a node in the early events triggered by the interactions between A. thaliana and the rhizobacterium WCS417. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
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- 2023
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3. Energy Hub Model for the Massive Adoption of Hydrogen in Power Systems
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Fabio Massaro, Maria Luisa Di Silvestre, Marco Ferraro, Francesco Montana, Eleonora Riva Sanseverino, and Salvatore Ruffino
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energy hub ,green hydrogen ,microgrid ,optimization ,renewables ,Technology - Abstract
A promising energy carrier and storage solution for integrating renewable energies into the power grid currently being investigated is hydrogen produced via electrolysis. It already serves various purposes, but it might also enable the development of hydrogen-based electricity storage systems made up of electrolyzers, hydrogen storage systems, and generators (fuel cells or engines). The adoption of hydrogen-based technologies is strictly linked to the electrification of end uses and to multicarrier energy grids. This study introduces a generic method to integrate and optimize the sizing and operation phases of hydrogen-based power systems using an energy hub optimization model, which can manage and coordinate multiple energy carriers and equipment. Furthermore, the uncertainty related to renewables and final demands was carefully assessed. A case study on an urban microgrid with high hydrogen demand for mobility demonstrates the method’s applicability, showing how the multi-objective optimization of hydrogen-based power systems can reduce total costs, primary energy demand, and carbon equivalent emissions for both power grids and mobility down to −145%. Furthermore, the adoption of the uncertainty assessment can give additional benefits, allowing a downsizing of the equipment.
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- 2024
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4. Legionella para-effectors target chromatin and promote bacterial replication
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Schator, Daniel, Mondino, Sonia, Berthelet, Jérémy, Di Silvestre, Cristina, Ben Assaya, Mathilde, Rusniok, Christophe, Rodrigues-Lima, Fernando, Wehenkel, Annemarie, Buchrieser, Carmen, and Rolando, Monica
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- 2023
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5. Proteomic Characterization of a 3D HER2+ Breast Cancer Model Reveals the Role of Mitochondrial Complex I in Acquired Resistance to Trastuzumab
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Ivana J. Tapia, Davide Perico, Virginia J. Wolos, Marcela S. Villaverde, Marianela Abrigo, Dario Di Silvestre, Pierluigi Mauri, Antonella De Palma, and Gabriel L. Fiszman
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HER2+ breast cancer ,Trastuzumab resistance ,3D cell culture ,proteomics ,systems biology ,mitochondria ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
HER2-targeted therapies, such as Trastuzumab (Tz), have significantly improved the clinical outcomes for patients with HER2+ breast cancer (BC). However, treatment resistance remains a major obstacle. To elucidate functional and metabolic changes associated with acquired resistance, we characterized protein profiles of BC Tz-responder spheroids (RSs) and non-responder spheroids (nRSs) by a proteomic approach. Three-dimensional cultures were generated from the HER2+ human mammary adenocarcinoma cell line BT-474 and a derived resistant cell line. Before and after a 15-day Tz treatment, samples of each condition were collected and analyzed by liquid chromatography–mass spectrometry. The analysis of differentially expressed proteins exhibited the deregulation of energetic metabolism and mitochondrial pathways. A down-regulation of carbohydrate metabolism and up-regulation of mitochondria organization proteins, the tricarboxylic acid cycle, and oxidative phosphorylation, were observed in nRSs. Of note, Complex I-related proteins were increased in this condition and the inhibition by metformin highlighted that their activity is necessary for nRS survival. Furthermore, a correlation analysis showed that overexpression of Complex I proteins NDUFA10 and NDUFS2 was associated with high clinical risk and worse survival for HER2+ BC patients. In conclusion, the non-responder phenotype identified here provides a signature of proteins and related pathways that could lead to therapeutic biomarker investigation.
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- 2024
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6. Lung-to-Heart Nano-in-Micro Peptide Promotes Cardiac Recovery in a Pig Model of Chronic Heart Failure
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Alogna, Alessio, Berboth, Leonhard, Faragli, Alessandro, Ötvös, Jens, lo Muzio, Francesco Paolo, di Mauro, Vittoria, Modica, Jessica, Quarta, Eride, Semmler, Lukas, Deißler, Peter Maximilian, Berger, Yannic Wanja, Tran, Khai Liem, de Marchi, Beatrice, Longinotti-Buitoni, Gianluigi, Degli Esposti, Lorenzo, Guillot, Etienne, Bazile, Didier, Iafisco, Michele, Dotti, Alessandro, Bang, Marie-Louise, de Luca, Claudio, Brandenberger, Christina, Benazzi, Louise, di Silvestre, Dario, de Palma, Antonella, Primeßnig, Uwe, Hohendanner, Felix, Perna, Simone, Buttini, Francesca, Colombo, Paolo, Mühlfeld, Christian, Steendijk, Paul, Mauri, Pierluigi, Tschöpe, Carsten, Borlaug, Barry, Pieske, Burkert M., Attanasio, Philipp, Post, Heiner, Heinzel, Frank R., and Catalucci, Daniele
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- 2024
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7. Legionella para-effectors target chromatin and promote bacterial replication
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Daniel Schator, Sonia Mondino, Jérémy Berthelet, Cristina Di Silvestre, Mathilde Ben Assaya, Christophe Rusniok, Fernando Rodrigues-Lima, Annemarie Wehenkel, Carmen Buchrieser, and Monica Rolando
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Science - Abstract
Abstract Legionella pneumophila replicates intracellularly by secreting effectors via a type IV secretion system. One of these effectors is a eukaryotic methyltransferase (RomA) that methylates K14 of histone H3 (H3K14me3) to counteract host immune responses. However, it is not known how L. pneumophila infection catalyses H3K14 methylation as this residue is usually acetylated. Here we show that L. pneumophila secretes a eukaryotic-like histone deacetylase (LphD) that specifically targets H3K14ac and works in synergy with RomA. Both effectors target host chromatin and bind the HBO1 histone acetyltransferase complex that acetylates H3K14. Full activity of RomA is dependent on the presence of LphD as H3K14 methylation levels are significantly decreased in a ∆lphD mutant. The dependency of these two chromatin-modifying effectors on each other is further substantiated by mutational and virulence assays revealing that the presence of only one of these two effectors impairs intracellular replication, while a double knockout (∆lphD∆romA) can restore intracellular replication. Uniquely, we present evidence for “para-effectors”, an effector pair, that actively and coordinately modify host histones to hijack the host response. The identification of epigenetic marks modulated by pathogens has the potential to lead to the development of innovative therapeutic strategies to counteract bacterial infection and strengthening host defences.
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- 2023
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8. An innovative strategy to investigate microbial protein modifications in a reliable fast and sensitive way: A therapy oriented proof of concept based on UV-C irradiation of SARS-CoV-2 spike protein
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Strizzi, Sergio, Bernardo, Letizia, D’Ursi, Pasqualina, Urbinati, Chiara, Bianco, Andrea, Limanaqi, Fiona, Manconi, Andrea, Milanesi, Maria, Macchi, Alberto, Di Silvestre, Dario, Cavalleri, Adalberto, Pareschi, Giovanni, Rusnati, Marco, Clerici, Mario, Mauri, PierLuigi, and Biasin, Mara
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- 2023
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9. Flexibility of grid interactive water heaters: The situation in the US
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Di Silvestre, M.L., Riva Sanseverino, E., Telaretti, E., and Zizzo, G.
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- 2023
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10. An innovative strategy to investigate microbial protein modifications in a reliable fast and sensitive way: A therapy oriented proof of concept based on UV-C irradiation of SARS-CoV-2 spike protein
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Sergio Strizzi, Letizia Bernardo, Pasqualina D’Ursi, Chiara Urbinati, Andrea Bianco, Fiona Limanaqi, Andrea Manconi, Maria Milanesi, Alberto Macchi, Dario Di Silvestre, Adalberto Cavalleri, Giovanni Pareschi, Marco Rusnati, Mario Clerici, PierLuigi Mauri, and Mara Biasin
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UV light ,Spike protein ,Proteomics ,Structural biology ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The characterization of modifications of microbial proteins is of primary importance to dissect pathogen lifecycle mechanisms and could be useful in identifying therapeutic targets. Attempts to solve this issue yielded only partial and non-exhaustive results. We developed a multidisciplinary approach by coupling in vitro infection assay, mass spectrometry (MS), protein 3D modelling, and surface plasma resonance (SPR). As a proof of concept, the effect of low UV-C (273 nm) irradiation on SARS-CoV-2 spike (S) protein was investigated. Following UV-C exposure, MS analysis identified, among other modifications, the disruption of a disulphide bond within the conserved S2 subunit of S protein. Computational analyses revealed that this bond breakage associates with an allosteric effect resulting in the generation of a closed conformation with a reduced ability to bind the ACE2 receptor. The UV-C-induced reduced affinity of S protein for ACE2 was further confirmed by SPR analyses and in vitro infection assays. This comprehensive approach pinpoints the S2 domain of S protein as a potential therapeutic target to prevent SARS-CoV-2 infection. Notably, this workflow could be used to screen a wide variety of microbial protein domains, resulting in a precise molecular fingerprint and providing new insights to adequately address future epidemics.
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- 2023
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11. An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking
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Toffali, Lara, D’Ulivo, Beatrice, Giagulli, Cinzia, Montresor, Alessio, Zenaro, Elena, Delledonne, Massimo, Rossato, Marzia, Iadarola, Barbara, Sbarbati, Andrea, Bernardi, Paolo, Angelini, Gabriele, Rossi, Barbara, Lopez, Nicola, Linke, Wolfgang A., Unger, Andreas, Di Silvestre, Dario, Benazzi, Louise, De Palma, Antonella, Motta, Sara, Constantin, Gabriela, Mauri, Pierluigi, and Laudanna, Carlo
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- 2023
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12. A blockchain platform for Demand Response in Mediterranean islands: a smart contract for remuneration.
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Maria Luisa Di Silvestre, Pierluigi Gallo, G. L. Restifo, Eleonora Riva Sanseverino, Giuseppe Sciumè, and Gaetano Zizzo
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- 2022
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13. Development of a Blockchain Network for DR Service Traceability and Certification
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Sciumè, G., Riva Sanseverino, E., Gallo, P., Di Silvestre, M. L., Zizzo, G., Riva Sanseverino, Eleonora, Editor-in-Chief, Amenta, Carlo, Series Editor, Carapezza, Marco, Series Editor, Chiodi, Marcello, Series Editor, Laghi, Andrea, Series Editor, Maresca, Bruno, Series Editor, Micale, Giorgio Domenico Maria, Series Editor, Mocciaro Li Destri, Arabella, Series Editor, Öchsner, Andreas, Series Editor, Piva, Mariacristina, Series Editor, Russo, Antonio, Series Editor, Seel, Norbert M., Series Editor, Sciumè, Giuseppe, editor, and Gallo, Pierluigi, editor
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- 2022
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14. Introduction
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Sciumè, G., Riva Sanseverino, E., Gallo, P., Di Silvestre, M. L., Zizzo, G., Musca, R., Vasquez, J. C., Guerrero, J. M., Riva Sanseverino, Eleonora, Editor-in-Chief, Amenta, Carlo, Series Editor, Carapezza, Marco, Series Editor, Chiodi, Marcello, Series Editor, Laghi, Andrea, Series Editor, Maresca, Bruno, Series Editor, Micale, Giorgio Domenico Maria, Series Editor, Mocciaro Li Destri, Arabella, Series Editor, Öchsner, Andreas, Series Editor, Piva, Mariacristina, Series Editor, Russo, Antonio, Series Editor, Seel, Norbert M., Series Editor, Sciumè, Giuseppe, editor, and Gallo, Pierluigi, editor
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- 2022
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15. An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking
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Lara Toffali, Beatrice D’Ulivo, Cinzia Giagulli, Alessio Montresor, Elena Zenaro, Massimo Delledonne, Marzia Rossato, Barbara Iadarola, Andrea Sbarbati, Paolo Bernardi, Gabriele Angelini, Barbara Rossi, Nicola Lopez, Wolfgang A. Linke, Andreas Unger, Dario Di Silvestre, Louise Benazzi, Antonella De Palma, Sara Motta, Gabriela Constantin, Pierluigi Mauri, and Carlo Laudanna
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CP: Immunology ,Biology (General) ,QH301-705.5 - Abstract
Summary: Response to multiple microenvironmental cues and resilience to mechanical stress are essential features of trafficking leukocytes. Here, we describe unexpected role of titin (TTN), the largest protein encoded by the human genome, in the regulation of mechanisms of lymphocyte trafficking. Human T and B lymphocytes express five TTN isoforms, exhibiting cell-specific expression, distinct localization to plasma membrane microdomains, and different distribution to cytosolic versus nuclear compartments. In T lymphocytes, the LTTN1 isoform governs the morphogenesis of plasma membrane microvilli independently of ERM protein phosphorylation status, thus allowing selectin-mediated capturing and rolling adhesions. Likewise, LTTN1 controls chemokine-triggered integrin activation. Accordingly, LTTN1 mediates rho and rap small GTPases activation, but not actin polymerization. In contrast, chemotaxis is facilitated by LTTN1 degradation. Finally, LTTN1 controls resilience to passive cell deformation and ensures T lymphocyte survival in the blood stream. LTTN1 is, thus, a critical and versatile housekeeping regulator of T lymphocyte trafficking.
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- 2023
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16. Divertor Tokamak Test facility project: status of design and implementation
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Francesco Romanelli, on behalf of DTT Contributors, D. Abate, E. Acampora, D. Agguiaro, R. Agnello, P. Agostinetti, M. Agostini, A. Aimetta, R. Albanese, G. Alberti, M. Albino, E. Alessi, S. Almaviva, M. Alonzo, R. Ambrosino, P. Andreoli, M. Angelone, M. Angelucci, C. Angioni, A. Angrisani Armenio, P. Antonini, D. Aprile, G. Apruzzese, M. Aquilini, G. Aragone, P. Arena, M. Ariola, G. Artaserse, L. Aucone, A. Augieri, F. Auriemma, J. Ayllon Guerola, N. Badodi, B. Baiocchi, L. Balbinot, C. Baldacchini, A. Balestri, T. Barberis, G. Barone, L. Barucca, M. Baruzzo, S. Begozzi, V. Belardi, F. Belli, A. Belpane, F. Beone, S. Bertolami, S. Bianucci, S. Bifaretti, S. Bigioni, W. Bin, P. Boccali, B. Boeswirth, E. Bogazzi, R. Bojoi, S. Bollanti, T. Bolzonella, F. Bombarda, M. Bonan, N. Bonanomi, A. Bonaventura, L. Boncagni, M. Bonesso, D. Bonfiglio, R. Bonifetto, D. Bonomi, D. Borgogno, T. Borzone, S. Botti, E. Boz, F. Braghin, M. Brena, S. Brezinsek, M. Brombin, A. Bruschi, S. Buonocore, P. Buratti, D. Busi, G. Calabrò, M. Caldora, G. Calvo, G. Camera, G. Campana, S. Candela, V. Candela, F. Cani, L. Cantone, F. Capaldo, S. Cappello, M. Caponero, S. Carchella, A. Cardinali, D. Carnevale, L. Carraro, C. Carrelli, V. Casalegno, I. Casiraghi, C. Castaldo, A. Castaldo, G. Castro, A. Carpignano, F. Causa, R. Cavazzana, M. Cavedon, M. Cavenago, M. Cecchini, S. Ceccuzzi, G. Celentano, L. Celona, C. Centioli, G.V. Centomani, S. Cesaroni, A.G. Chiariello, R. Chomicz, C. Cianfarani, F. Cichocki, M. Cinque, A. Cioffi, M. Ciotti, M. Cipriani, S. Ciufo, V. Claps, G. Claps, V. Coccorese, D. Coccorese, A. Colangeli, T. Coltella, F. Consoli, F. Cordella, D. Corradini, O. Costa, F. Crea, A. Cremona, F. Crescenzi, F. Crisanti, G. Cristofari, G. Croci, A. Cucchiaro, D. D’Ambrosio, M. Dal Molin, M. Dalla Palma, F. Danè, C. Day, M. De Angeli, V. De Leo, R. De Luca, E. De Marchi, G. De Marzi, G. De Masi, E. De Nardi, C. De Piccoli, G. De Sano, M. De Santis, G. De Tommasi, A. Del Nevo, A. Delfino, A. Della Corte, P. Deodati, S. Desiderati, E. Di Ferdinando, M.G. Di Florio, G. Di Gironimo, L.E. Di Grazia, V. Di Marzo, F. Di Paolo, E. Di Pietro, M. Di Pietrantonio, M. Di Prinzio, A. Di Silvestre, A. Di Zenobio, R. Dima, A. Domenichelli, A. Doria, G. Dose, S. Dubbioso, S. Dulla, I. Duran, M. Eboli, M. Elitropi, E. Emanuelli, B. Esposito, P. Ettorre, C. Fabbri, F. Fabbri, M. Fadone, M.M. Faggiano, F. Falcioni, M.V. Falessi, F. Fanale, P. Fanelli, A. Fassina, M. Favaretto, G. Favero, M. Ferraris, F. Ferrazza, C. Ferretti, A. Ferro, N. Ferron, C. Fiamozzi Zignani, L. Figini, F. Filippi, M. Filippini, A. Fimiani, M. Fincato, F. Fiorenza, D. Fiorucci, D. Flammini, F. Flora, N. Fonnesu, P. Franz, L. Frassinetti, A. Frattolillo, R. Freda, R. Fresa, A. Frescura, P. Frosi, M. Fulici, M. Furno Palumbo, V. Fusco, P. Fusco, L. Gabellier, P. Gaetani, E. Gaio, E. Gajetti, A. Galatà, J. Galdon Quiroga, D.L. Galindo Huertas, S. Gammino, G. Gandolfo, S. Garavaglia, J. Garcia Lopez, M. Garcia Muñoz, P. Gaudio, M. Gelfusa, G. Gervasini, L. Giannini, M. Giarrusso, C. Gil, F. Giorgetti, E. Giovannozzi, G. Giruzzi, L. Giudicotti, M. Gobbin, G. Gorini, G. Granucci, D. Grasso, T. Grasso, S. Grazioso, H. Greuner, G. Griva, G. Grosso, S. Guerini, J.P. Gunn, V. Hauer, J. Hidalgo Salaverri, M. Hoppe, M. Houry, M. Hoelzl, A. Iaboni, M. Iafrati, A. Iaiunese, V. Imbriani, D. Indrigo, P. Innocente, F. Koechl, B. Končar, A. Kryzhanovskyy, L. Laguardia, D.A. Lampasi, C. Lanchi, F. Lanzotti, A. Lanzotti, M. Laquaniti, F. Leone, J. Li, M. Libè, F. Lisanti, D. Liuzza, F. Locati, R. Lombroni, R. Lorenzini, P. Lorusso, L. Lotto, J. Loureiro, F. Lucca, T. Luda Di Cortemiglia, P. Maccari, G. Maddaluno, S. Magagnino, G. Manca, A. Mancini, P. Mandalà, B. Mandolesi, F. Mandrile, G. Manduchi, S. Manfrin, M. Manganelli, P. Mantica, G. Marchiori, N. Marconato, G. Marelli, A. Mariani, A. Marin, R. Marinari, M. Marinelli, F. Marino, P. Marino, D. Marocco, R. Marsilio, E. Martelli, P. Martin, F. Martinelli, G. Martini, R. Martone, A. Marucci, D. Marzullo, V. Masala, D. Mascali, F. Mascari, A. Masi, N. Massanova, S. Mastrostefano, M. Mattei, G. Mauro, S. Mauro, C. Meineri, L. Melaragni, A. Mele, P. Meller, S. Meloni, I. Menicucci, G. Messina, L. Mezi, G. Miccichè, M. Micheletti, S. Migliori, D. Milanesio, F. Milazzo, R. Milazzo, P. Minelli, S. Minucci, F. Mirizzi, M. Missirlian, D. Monarca, C. Monti, M. Mori, A. Moriani, L. Morici, A. Moro, F. Moro, P. Mosetti, R. Mozzillo, A. Murari, A. Muraro, D. Murra, P. Muscente, S. Musumeci, L. Muzzi, G.F. Nallo, F. Napoli, E. Nardon, E. Naselli, R. Neu, M. Nocente, M. Notazio, S. Nowak, E. Ocello, A. Oliva, V. Orsetti, A. Orsini, F.P. Orsitto, M. Ortino, M. Ottavi, G. Paccagnella, D. Pacella, I. Pagani, N. Paganucci, A. Pagliaro, V. Palazzolo, M. Palermo, S. Palomba, F. Panza, D. Paoletti, M. Parisi, R. Pasqualotto, S. Passarello, M. Passoni, T. Patton, L. Pelliccia, A. Peloso, A. Pepato, E. Perelli, A. Perencin, S. Peruzzo, A. Pesenti, N. Pedroni, P. Petrolini, V. Piergotti, A. Pidatella, L. Pigatto, M. Pillon, T. Pinna, S. Pipolo, S. Piras, C. Piron, L. Piron, A. Pironti, M. Pistilli, D. Placido, A. Pizzuto, P. Platania, A. Polimadei, F. Pollastrone, G.M. Polli, N. Pomaro, F. Pompili, C. Ponti, F. Porcelli, V. Prandelli, A. Previti, A. Princiotta, G. Pucino, F. Quaglia, A. Quercia, F. Raffaelli, G. Ramogida, G. Ranieri, B. Raspante, D. Ravarotto, G.L. Ravera, A. Reale, P. Rebesan, M. Recchia, D. Regine, F. Renno, B. Riccardi, D. Ricci, D. Rigamonti, M. Ripani, N. Rispoli, S. Roccella, G. Rocchi, H. Roche, M. Romanato, F. Romanelli, G. Romanelli, R. Romaniello, A. Romano, M. Romano, R. Romano, R. Rossi, G. Rubinacci, G. Rubino, S. Rubino, J. Rueda Rueda, A. Rufoloni, C. Salvia, P. Salvini, M. Scarpari, A. Salvitti, L. Salvò, S. Sandri, F. Santoro, A. Satriano, L. Savoldi, C. Scardino, G. Schettini, S. Schmuck, J. Scionti, M. Scisciò, M. Scungio, K. Sedlak, L. Senni, G. Sias, A. Sibio, A. Simonetto, L. Singh, A. Sirignano, C. Sozzi, I. Spada, S. Spagnolo, L. Spinicci, G. Spizzo, M. Spolaore, C. Stefanini, H. Strobel, F. Subba, F. Taccogna, B. Taheri, C. Tantos, A. Tarallo, M. Tarantino, G. Tardini, M. Tardocchi, P. Tarfila, A. Tenaglia, C. Terlizzi, D. Terranova, D. Testa, E. Testa, R. Testoni, V. Toigo, G. Torrisi, A. Trotta, G. Trovato, E. Tsitrone, A. Tuccillo, O. Tudisco, M. Turcato, S. Turtù, A. Uccello, M. Ugoletti, O. Uras, M. Uras, M. Utili, V. Vaccaro, F. Valentini, L. Valletti, M. Valisa, D. Van Eester, D. Vanzan, E. Vassallo, G. Vecchi, M. Vellucci, I. Venneri, G. Ventura, M. Veranda, L. Verdini, C. Verona, G. Verona Rinati, F. Veronese, N. Vianello, F. Viganò, O. Villano, R. Villari, F. Villone, P. Vincenzi, V. Vitale, F. Vivio, G. Vlad, M. Wischmeier, H.S. Wu, I. Wyss, R. Zanino, B. Zaniol, F. Zanon, A. Zappatore, G. Zavarise, P. Zito, A. Zoppoli, M. Zucchetti, M. Zuin, and P. Zumbolo
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divertor ,exhaust ,plasma scenarios ,Nuclear and particle physics. Atomic energy. Radioactivity ,QC770-798 - Abstract
An overview is presented of the progress since 2021 in the construction and scientific programme preparation of the Divertor Tokamak Test (DTT) facility. Licensing for building construction has been granted at the end of 2021. Licensing for Cat. A radiologic source has been also granted in 2022. The construction of the toroidal field magnet system is progressing. The prototype of the 170 GHz gyrotron has been produced and it is now under test on the FALCON facility. The design of the vacuum vessel, the poloidal field coils and the civil infrastructures has been completed. The shape of the first DTT divertor has been agreed with EUROfusion to test different plasma and exhaust scenarios: single null, double null, X-divertor and negative triangularity plasmas. A detailed research plan is being elaborated with the involvement of the EUROfusion laboratories.
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- 2024
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17. Spectroscopic investigation of faeces with surface-enhanced Raman scattering: a case study with coeliac patients on gluten-free diet
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Fornasaro, Stefano, Esposito, Alessandro, Florian, Fiorella, Pallavicini, Alberto, De Leo, Luigina, Not, Tarcisio, Lagatolla, Cristina, Mezzarobba, Marica, Di Silvestre, Alessia, Sergo, Valter, and Bonifacio, Alois
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- 2022
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18. Integrated Strategies for a Holistic View of Extracellular Vesicles
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Valeria Rondelli, Sally Helmy, Giulia Passignani, Pietro Parisse, and Dario Di Silvestre
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Chemistry ,QD1-999 - Published
- 2022
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19. Reduced G protein signaling despite impaired internalization and β-arrestin recruitment in patients carrying a CXCR4Leu317fsX3 mutation causing WHIM syndrome
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Rajesh Kumar, Samantha Milanesi, Martyna Szpakowska, Laura Dotta, Dario Di Silvestre, Anna Maria Trotta, Anna Maria Bello, Mauro Giacomelli, Manuela Benedito, Joana Azevedo, Alexandra Pereira, Emilia Cortesao, Alessandro Vacchini, Alessandra Castagna, Marinella Pinelli, Daniele Moratto, Raffaella Bonecchi, Massimo Locati, Stefania Scala, Andy Chevigné, Elena M. Borroni, and Raffaele Badolato
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Cell biology ,Immunology ,Medicine - Abstract
WHIM syndrome is an inherited immune disorder caused by an autosomal dominant heterozygous mutation in CXCR4. The disease is characterized by neutropenia/leukopenia (secondary to retention of mature neutrophils in bone marrow), recurrent bacterial infections, treatment-refractory warts, and hypogammaglobulinemia. All mutations reported in WHIM patients lead to the truncations in the C-terminal domain of CXCR4, R334X being the most frequent. This defect prevents receptor internalization and enhances both calcium mobilization and ERK phosphorylation, resulting in increased chemotaxis in response to the unique ligand CXCL12. Here, we describe 3 patients presenting neutropenia and myelokathexis, but normal lymphocyte count and immunoglobulin levels, carrying what we believe to be a novel Leu317fsX3 mutation in CXCR4, leading to a complete truncation of its intracellular tail. The analysis of the L317fsX3 mutation in cells derived from patients and in vitro cellular models reveals unique signaling features in comparison with R334X mutation. The L317fsX3 mutation impairs CXCR4 downregulation and β-arrestin recruitment in response to CXCL12 and reduces other signaling events — including ERK1/2 phosphorylation, calcium mobilization, and chemotaxis — all processes that are typically enhanced in cells carrying the R334X mutation. Our findings suggest that, overall, the L317fsX3 mutation may be causative of a form of WHIM syndrome not associated with an augmented CXCR4 response to CXCL12.
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- 2023
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20. The use of complementary alternative medicines in advanced cancer patients followed at home
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Mercadante, Sebastiano, Bellavia, Giuseppe, Cascio, Alessio Lo, Dabbene, Marcella, di Silvestre, Grazia, and Casuccio, Alessandra
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- 2022
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21. Pattern and characteristics of patients admitted to a hospice connected with an acute palliative care unit in a comprehensive cancer center
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Mercadante, Sebastiano, Giuliana, Fausto, Bellingardo, Rosanna, Albegiani, Gianluca, Di Silvestre, Grazia, and Casuccio, Alessandra
- Published
- 2022
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22. Energy Hub Model for the Massive Adoption of Hydrogen in Power Systems.
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Massaro, Fabio, Di Silvestre, Maria Luisa, Ferraro, Marco, Montana, Francesco, Riva Sanseverino, Eleonora, and Ruffino, Salvatore
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GREEN fuels , *ENERGY consumption , *ELECTRIC power distribution grids , *CLEAN energy , *RENEWABLE energy sources - Abstract
A promising energy carrier and storage solution for integrating renewable energies into the power grid currently being investigated is hydrogen produced via electrolysis. It already serves various purposes, but it might also enable the development of hydrogen-based electricity storage systems made up of electrolyzers, hydrogen storage systems, and generators (fuel cells or engines). The adoption of hydrogen-based technologies is strictly linked to the electrification of end uses and to multicarrier energy grids. This study introduces a generic method to integrate and optimize the sizing and operation phases of hydrogen-based power systems using an energy hub optimization model, which can manage and coordinate multiple energy carriers and equipment. Furthermore, the uncertainty related to renewables and final demands was carefully assessed. A case study on an urban microgrid with high hydrogen demand for mobility demonstrates the method's applicability, showing how the multi-objective optimization of hydrogen-based power systems can reduce total costs, primary energy demand, and carbon equivalent emissions for both power grids and mobility down to −145%. Furthermore, the adoption of the uncertainty assessment can give additional benefits, allowing a downsizing of the equipment. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Integration of Omics Data and Network Models to Unveil Negative Aspects of SARS-CoV-2, from Pathogenic Mechanisms to Drug Repurposing
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Letizia Bernardo, Andrea Lomagno, Pietro Luigi Mauri, and Dario Di Silvestre
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omics ,networks ,drug repurposing ,SARS-CoV-2 ,COVID-19 ,Biology (General) ,QH301-705.5 - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the COVID-19 health emergency, affecting and killing millions of people worldwide. Following SARS-CoV-2 infection, COVID-19 patients show a spectrum of symptoms ranging from asymptomatic to very severe manifestations. In particular, bronchial and pulmonary cells, involved at the initial stage, trigger a hyper-inflammation phase, damaging a wide range of organs, including the heart, brain, liver, intestine and kidney. Due to the urgent need for solutions to limit the virus’ spread, most efforts were initially devoted to mapping outbreak trajectories and variant emergence, as well as to the rapid search for effective therapeutic strategies. Samples collected from hospitalized or dead COVID-19 patients from the early stages of pandemic have been analyzed over time, and to date they still represent an invaluable source of information to shed light on the molecular mechanisms underlying the organ/tissue damage, the knowledge of which could offer new opportunities for diagnostics and therapeutic designs. For these purposes, in combination with clinical data, omics profiles and network models play a key role providing a holistic view of the pathways, processes and functions most affected by viral infection. In fact, in addition to epidemiological purposes, networks are being increasingly adopted for the integration of multiomics data, and recently their use has expanded to the identification of drug targets or the repositioning of existing drugs. These topics will be covered here by exploring the landscape of SARS-CoV-2 survey-based studies using systems biology approaches derived from omics data, paying particular attention to those that have considered samples of human origin.
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- 2023
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24. INFECCIÓN DE DISPOSITIVO INTRACARDIACO POR CANDIDA LUSITANIAE
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D. García Rosado, M.M. Alonso Soca, C. Borja-Goyeneche, M. di Silvestre Alonso, P.C. Prada Arrondo, A. Madueño Alonso, M.J. García González, M.M. Martín Cabeza, I.D. Poveda Pinedo, and J. La Calzada Almeida
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Medicine ,Surgery ,RD1-811 - Published
- 2023
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25. MCT1-dependent energetic failure and neuroinflammation underlie optic nerve degeneration in Wolfram syndrome mice
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Greta Rossi, Gabriele Ordazzo, Niccolò N Vanni, Valerio Castoldi, Angelo Iannielli, Dario Di Silvestre, Edoardo Bellini, Letizia Bernardo, Serena G Giannelli, Mirko Luoni, Sharon Muggeo, Letizia Leocani, PierLuigi Mauri, and Vania Broccoli
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eye ,retinal development ,glial cell ,astrocytes ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Wolfram syndrome 1 (WS1) is a rare genetic disorder caused by mutations in the WFS1 gene leading to a wide spectrum of clinical dysfunctions, among which blindness, diabetes, and neurological deficits are the most prominent. WFS1 encodes for the endoplasmic reticulum (ER) resident transmembrane protein wolframin with multiple functions in ER processes. However, the WFS1-dependent etiopathology in retinal cells is unknown. Herein, we showed that Wfs1 mutant mice developed early retinal electrophysiological impairments followed by marked visual loss. Interestingly, axons and myelin disruption in the optic nerve preceded the degeneration of the retinal ganglion cell bodies in the retina. Transcriptomics at pre-degenerative stage revealed the STAT3-dependent activation of proinflammatory glial markers with reduction of the homeostatic and pro-survival factors glutamine synthetase and BDNF. Furthermore, label-free comparative proteomics identified a significant reduction of the monocarboxylate transport isoform 1 (MCT1) and its partner basigin that are highly enriched on retinal glia and myelin-forming oligodendrocytes in optic nerve together with wolframin. Loss of MCT1 caused a failure in lactate transfer from glial to neuronal cell bodies and axons leading to a chronic hypometabolic state. Thus, this bioenergetic impairment is occurring concurrently both within the axonal regions and cell bodies of the retinal ganglion cells, selectively endangering their survival while impacting less on other retinal cells. This metabolic dysfunction occurs months before the frank RGC degeneration suggesting an extended time-window for intervening with new therapeutic strategies focused on boosting retinal and optic nerve bioenergetics in WS1.
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- 2023
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26. Interatrial septum dissection, severe aortic stenosis, and paravalvular mitral regurgitation: A rare combination and their hemodynamic interrelationship
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Di Silvestre M, Muñoz‐Rodríguez R, Tindaya‐Herraiz A, Bosa‐Ojeda F, Barragan‐Acea A, Bonilla A, and Lacalzada‐Almeida J
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Atrial dissection ,Hemolytic anemia ,Mitral valve regurgitation ,Mitral valve replacement ,Transesophageal echocardiography ,Transcatheter Aortic Valve Implantation ,Medicine ,Medicine (General) ,R5-920 - Abstract
Abstract Left atrial wall dissection is a rare entity with controversial management approaches. We report the case of an 85‐year‐old woman with a mitral bioprosthetic admitted for heart failure. Diagnosis of atrial septum dissection, severe aortic stenosis, and paravalvular mitral regurgitation was established. A percutaneous approach provided clinical improvements.
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- 2023
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27. Trojan-horse silk fibroin nanocarriers loaded with a re-call antigen to redirect immunity against cancer
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Luca Mastracci, Patrizio Castagnola, Elia Bari, Francesca Ferrera, Tiziana Altosole, Sara Perteghella, Pierluigi Mauri, Rossana Rossi, Giulia Passignani, Martina Galati, Giuseppina Iliana Astone, Maddalena Mastrogiacomo, Daniela Fenoglio, Dario Di Silvestre, Maria Luisa Torre, and Gilberto Filaci
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background The current challenge for immunotherapies is to generate effective antitumor immunity. Since tumor immune escape mechanisms do not impact pre-existing and consolidated immune responses, we tested the hypothesis of redirecting a pregenerated immunity to cancer: to recall a non-tumor antigen response against the tumor, silk fibroin nanoparticles (SFNs) have been selected as ‘Trojan-horse’ carriers, promoting the antigen uptake by the tumor cells.Methods SFNs have been loaded with either ovalbumin (OVA) or CpG oligonucleotide (CpG) as antigen or adjuvant, respectively. In vitro uptake of SFNs by tumor (B16/F10 melanoma and MB49 bladder cancer) or dendritic cells, as well as the presence of OVA-specific T cells in splenic and tumor-infiltrating lymphocytes, were assessed by cytometric analyses. Proof-of-concept of in vivo efficacy was achieved in an OVA-hyperimmune B16/F10 murine melanoma model: SFNs-OVA or SFNs-CpG were injected, separately or in association, into the subcutaneous peritumoral area. Cancer dimensions/survival time were monitored, while, at the molecular level, system biology approaches based on graph theory and experimental proteomic data were performed.Results SFNs were efficiently in vitro uptaken by cancer and dendritic cells. In vivo peritumor administration of SFNs-OVA redirected OVA-specific cytotoxic T cells intratumorally. Proteomics and systems biology showed that peritumoral treatment with either SFNs-OVA or SFNs-CpG dramatically modified tumor microenvironment with respect to the control (CTR), mainly involving functional modules and hubs related to angiogenesis, inflammatory mediators, immune function, T complex and serpins expression, redox homeostasis, and energetic metabolism. Both SFNs-OVA and SFNs-CpG significantly delayed melanoma growth/survival time, and their effect was additive.Conclusions Both SFNs-OVA and SFNs-CpG induce effective anticancer response through complementary mechanisms and show the efficacy of an innovative active immunotherapy approach based on the redirection of pre-existing immunity against cancer cells. This approach could be universally applied for solid cancer treatments if translated into the clinic using re-call antigens of childhood vaccination.
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- 2023
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28. Autonomous role of Wiskott-Aldrich syndrome platelet deficiency in inducing autoimmunity and inflammation
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Sereni, Lucia, Castiello, Maria Carmina, Marangoni, Francesco, Anselmo, Achille, di Silvestre, Dario, Motta, Sara, Draghici, Elena, Mantero, Stefano, Thrasher, Adrian J, Giliani, Silvia, Aiuti, Alessandro, Mauri, Pierluigi, Notarangelo, Luigi D, Bosticardo, Marita, and Villa, Anna
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Biomedical and Clinical Sciences ,Immunology ,Clinical Research ,Rare Diseases ,2.1 Biological and endogenous factors ,Aetiology ,Inflammatory and immune system ,Adolescent ,Adult ,Animals ,Autoimmunity ,Blood Platelets ,CD40 Ligand ,Child ,Child ,Preschool ,Female ,Humans ,Infant ,Inflammation ,Mice ,Inbred C57BL ,Mice ,Knockout ,Platelet Count ,Wiskott-Aldrich Syndrome ,Wiskott-Aldrich Syndrome Protein ,Young Adult ,Wiskott-Aldrich syndrome ,platelet deficiency ,CD40 ligand ,autoantibodies ,autoimmunity ,Allergy - Abstract
BACKGROUND:Wiskott-Aldrich syndrome (WAS) is an X-linked immunodeficiency characterized by eczema, infections, and susceptibility to autoimmunity and malignancies. Thrombocytopenia is a constant finding, but its pathogenesis remains elusive. OBJECTIVE:To dissect the basis of the WAS platelet defect, we used a novel conditional mouse model (CoWas) lacking Wiskott-Aldrich syndrome protein (WASp) only in the megakaryocytic lineage in the presence of a normal immunologic environment, and in parallel we analyzed samples obtained from patients with WAS. METHODS:Phenotypic and functional characterization of megakaryocytes and platelets in mutant CoWas mice and patients with WAS with and without autoantibodies was performed. Platelet antigen expression was examined through a protein expression profile and cluster proteomic interaction network. Platelet immunogenicity was tested by using ELISAs and B-cell and platelet cocultures. RESULTS:CoWas mice showed increased megakaryocyte numbers and normal thrombopoiesis in vitro, but WASp-deficient platelets had short lifespan and high expression of activation markers. Proteomic analysis identified signatures compatible with defects in cytoskeletal reorganization and metabolism yet surprisingly increased antigen-processing capabilities. In addition, WASp-deficient platelets expressed high levels of surface and soluble CD40 ligand and were capable of inducing B-cell activation in vitro. WASp-deficient platelets were highly immunostimulatory in mice and triggered the generation of antibodies specific for WASp-deficient platelets, even in the context of a normal immune system. Patients with WAS also showed platelet hyperactivation and increased plasma soluble CD40 ligand levels correlating with the presence of autoantibodies. CONCLUSION:Overall, these findings suggest that intrinsic defects in WASp-deficient platelets decrease their lifespan and dysregulate immune responses, corroborating the role of platelets as modulators of inflammation and immunity.
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- 2018
29. Phosphoproteomic mapping of CCR5 and ACKR2 signaling properties
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Alessandro Vacchini, Elisa Maffioli, Dario Di Silvestre, Cinzia Cancellieri, Samantha Milanesi, Simona Nonnis, Sabrina Badanai, Pierluigi Mauri, Armando Negri, Massimo Locati, Gabriella Tedeschi, and Elena Monica Borroni
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ACKR2 ,CCR5 ,signaling ,SILAC ,phosphoproteome ,Biology (General) ,QH301-705.5 - Abstract
ACKR2 is an atypical chemokine receptor which is structurally uncoupled from G proteins and is unable to activate signaling pathways used by conventional chemokine receptors to promote cell migration. Nonetheless, ACKR2 regulates inflammatory and immune responses by shaping chemokine gradients in tissues via scavenging inflammatory chemokines. To investigate the signaling pathways downstream to ACKR2, a quantitative SILAC-based phosphoproteomic analysis coupled with a systems biology approach with network analysis, was carried out on a HEK293 cell model expressing either ACKR2 or its conventional counterpart CCR5. The model was stimulated with the common agonist CCL3L1 for short (3 min) and long (30 min) durations. As expected, many of the identified proteins are known to participate in conventional signal transduction pathways and in the regulation of cytoskeleton dynamics. However, our analyses revealed unique phosphorylation and network signatures, suggesting roles for ACKR2 other than its scavenger activity. In conclusion, the mapping of phosphorylation events at a holistic level indicated that conventional and atypical chemokine receptors differ in signaling properties. This provides an unprecedented level of detail in chemokine receptor signaling and identifying potential targets for the regulation of ACKR2 and CCR5 function.
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- 2022
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30. Awake clavicle and arm surgery in critical ill patient: how to make it possible
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SCIAMANNA, Paola, primary, DI SILVESTRE, Gabriele, additional, GODERECCI, Remo, additional, MURGIA, Federico, additional, VENTURONI, Federica, additional, MARINANGELI, Franco, additional, and SCIMIA, Paolo, additional
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- 2024
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31. Energy self-consumers and renewable energy communities in Italy: New actors of the electric power systems
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Di Silvestre, Maria Luisa, Ippolito, Mariano Giuseppe, Sanseverino, Eleonora Riva, Sciumè, Giuseppe, and Vasile, Antony
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- 2021
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32. Gender Differences in Osteoporosis: A Single- Center Observational Study
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Massimo De Martinis, Maria Maddalena Sirufo, Matteo Polsinelli, Giuseppe Placidi, Daniela Di Silvestre, and Lia Ginaldi
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aging ,bone ,bone density ,gender ,men ,osteoporosis ,Medicine ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Purpose: Osteoporosis affects more than 200 million people worldwide: its prevalence increases with age and is actually growing due to the constant population aging. Women are at greater risk than men, but in recent years it has become increasingly evident that osteoporosis represents a significantly important problem also for men. However, osteoporosis in men is still poorly studied, underdiagnosed and inadequately treated. Materials and Methods: We conducted an observational study to identify any gender disparities in osteoporosis screening. For this purpose we observed people consecutively admitted at our Outpatient Service for the Diagnosis of Osteoporosis during the last 3 years. Patients underwent clinical and laboratory assessment and bone mineral density (BMD) measurements by dual-energy X-ray absorptiometry. Bone turnover serum markers have been evaluated and stratified according to gender. Results: Out of 3,752 patients, 2,376 subjects who met the inclusion criteria were identified. As expected, the great majority (94.5%) of the screened subjects were women and only 5.4% were men. Women exhibited lower BMD compared to men (Tscore values: -2.33±1.14 vs. -1.31±1.55; p
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- 2021
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33. Ductal Carcinoma In Situ (DCIS) and Microinvasive DCIS: Role of Surgery in Early Diagnosis of Breast Cancer
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Francesca Magnoni, Beatrice Bianchi, Giovanni Corso, Erica Anna Alloggio, Susanna Di Silvestre, Giuliarianna Abruzzese, Virgilio Sacchini, Viviana Galimberti, and Paolo Veronesi
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breast cancer ,prevention ,ductal carcinoma in situ ,DCIS ,microinvasion ,microinvasive breast cancer ,Medicine - Abstract
Advances in treatments, screening, and awareness have led to continually decreasing breast cancer-related mortality rates in the past decades. This achievement is coupled with early breast cancer diagnosis. Ductal carcinoma in situ (DCIS) and microinvasive breast cancer have increasingly been diagnosed in the context of mammographic screening. Clinical management of DCIS is heterogenous, and the clinical significance of microinvasion in DCIS remains elusive, although microinvasive DCIS (DCIS-Mi) is distinct from “pure” DCIS. Upfront surgery has a fundamental role in the overall treatment of these breast diseases. The growing number of screen-detected DCIS diagnoses with clinicopathological features of low risk for local recurrence (LR) allows more conservative surgical options, followed by personalised adjuvant radiotherapy plans. Furthermore, studies are underway to evaluate the validity of surgery omission in selected low-risk categories. Nevertheless, the management, the priority of axillary surgical staging, and the prognosis of DCIS-Mi remain the subject of debate, demonstrating how the paucity of data still necessitates adequate studies to provide conclusive guidelines. The current scientific scenario for DCIS and DCIS-Mi surgical approach consists of highly controversial and diversified sources, which this narrative review will delineate and clarify.
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- 2023
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34. Development of a Blockchain Network for DR Service Traceability and Certification
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Sciumè, G., primary, Riva Sanseverino, E., additional, Gallo, P., additional, Di Silvestre, M. L., additional, and Zizzo, G., additional
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- 2022
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35. Introduction
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Sciumè, G., primary, Riva Sanseverino, E., additional, Gallo, P., additional, Di Silvestre, M. L., additional, Zizzo, G., additional, Musca, R., additional, Vasquez, J. C., additional, and Guerrero, J. M., additional
- Published
- 2022
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36. Analysing omics data sets with weighted nodes networks (WNNets)
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Gabriele Tosadori, Dario Di Silvestre, Fausto Spoto, Pierluigi Mauri, Carlo Laudanna, and Giovanni Scardoni
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Medicine ,Science - Abstract
Abstract Current trends in biomedical research indicate data integration as a fundamental step towards precision medicine. In this context, network models allow representing and analysing complex biological processes. However, although effective in unveiling network properties, these models fail in considering the individual, biochemical variations occurring at molecular level. As a consequence, the analysis of these models partially loses its predictive power. To overcome these limitations, Weighted Nodes Networks (WNNets) were developed. WNNets allow to easily and effectively weigh nodes using experimental information from multiple conditions. In this study, the characteristics of WNNets were described and a proteomics data set was modelled and analysed. Results suggested that degree, an established centrality index, may offer a novel perspective about the functional role of nodes in WNNets. Indeed, degree allowed retrieving significant differences between experimental conditions, highlighting relevant proteins, and provided a novel interpretation for degree itself, opening new perspectives in experimental data modelling and analysis. Overall, WNNets may be used to model any high-throughput experimental data set requiring weighted nodes. Finally, improving the power of the analysis by using centralities such as betweenness may provide further biological insights and unveil novel, interesting characteristics of WNNets.
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- 2021
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37. Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healing
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Erratico, Silvia, Belicchi, Marzia, Meregalli, Mirella, Di Silvestre, Dario, Tripodi, Luana, De Palma, Antonella, Jones, Rebecca, Ferrari, Emanuele, Porretti, Laura, Trombetta, Elena, Merlo, Giorgio R., Mauri, Pierluigi, and Torrente, Yvan
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- 2022
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38. Caloric Restriction Promotes Immunometabolic Reprogramming Leading to Protection from Tuberculosis
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Palma, Carla, La Rocca, Claudia, Gigantino, Vincenzo, Aquino, Gabriella, Piccaro, Giovanni, Di Silvestre, Dario, Brambilla, Francesca, Rossi, Rossana, Bonacina, Fabrizia, Lepore, Maria Teresa, Audano, Matteo, Mitro, Nico, Botti, Gerardo, Bruzzaniti, Sara, Fusco, Clorinda, Procaccini, Claudio, De Rosa, Veronica, Galgani, Mario, Alviggi, Carlo, Puca, Annibale, Grassi, Fabio, Rezzonico-Jost, Tanja, Norata, Giuseppe Danilo, Mauri, Pierluigi, Netea, Mihai G., de Candia, Paola, and Matarese, Giuseppe
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- 2021
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39. A Method for Assessing the Feasibility of Integrating Planned Unidirectional EV Chargers into the Distribution Grid: A Case Study in Danang, Vietnam
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Nam Hoai Nguyen, Quynh T. Tran, Thao V. Nguyen, Nam Tran, Leon Roose, Saeed Sepasi, and Maria Luisa Di Silvestre
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electric vehicles ,unidirectional charger ,distribution grid ,fast chargers ,Technology - Abstract
The journey towards transportation electrification started with electric vehicles and has attracted more and more attention on a global scale in recent years. EVs are seen as a substantial, effective, and urgent solution for transportation electrification. In this paper, we investigate the operation requirements for integrating charger stations into the distribution grid in Vietnam. We also propose a simple evaluation method for assessing the feasibility of integrating planned unidirectional EV chargers into the distribution grid. The assessment method is applied to two main distribution feeders in Danang, Vietnam, where the new charger stations are already planned to be deployed in 2025 and 2030. The results showed that with addition of pre-planned EV chargers, both feeders still meet operation requirements in 2025 and 2030. However, the feeder with voltage indices close to the limit needs to be considered for an upgrade in configuration.
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- 2023
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40. Magnetic Resonance Imaging in the Study of Cardiac Masses: A Case Series
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Jorge Joaquín Castro-Martín, Mauro Andrés Di Silvestre-Alonso, Manuel Rivero-García, Rebeca Muñoz-Rodríguez, María Manuela Izquierdo-Gómez, Flor Baeza-Garzón, and Juan Lacalzada-Almeida
- Subjects
cardiac masses ,cardiac tumors ,cardiac metastases ,myxoma ,cardiac magnetic resonance imaging ,intracardiac thrombi ,Medicine (General) ,R5-920 - Abstract
Cardiac masses are currently studied using multimodality imaging. For diagnosis, different imaging techniques that can provide complementary information are used. Cardiac magnetic resonance imaging (MRI) has become a fundamental tool for this type of pathology owing to its ability to provide tissue characterization, spatial accuracy, and the anatomic relationships of the different structures. This study presents a series of four clinical cases with an initial diagnosis of a cardiac mass. All cases were evaluated at a single center, and patients were aged 57 to 72 years. An etiological study was conducted on all patients using different imaging techniques, including MRI. This study describes the diagnostic and therapeutic procedures of the four cases, which included two intracardiac metastases and two benign tumors. Cardiac MRI was decisive in the diagnostic process, determining the clinical decision-making in all four cases. Cardiac MRI has emerged as a pivotal technique in the diagnosis of cardiac masses. It can provide a highly accurate histological diagnosis without the need for invasive techniques.
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- 2023
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41. Critical Assessments of the Potential for Integrating Renewable Energy into Isolated Grids on Vietnamese Islands: The Case of the An-Binh Grid
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Hang Thi-Thuy Le, Eleonora Riva Sanseverino, Ninh Quang Nguyen, Maria Luisa Di Silvestre, Salvatore Favuzza, Binh Doan Van, and Rossano Musca
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photovoltaic ,isolated networks ,dynamic security ,NEPLAN ,Vietnam ,Technology - Abstract
Renewable electricity for off-grid areas is widely seen as one of the top choices in supporting local economic development in most countries, and so is Vietnam. Over the years, many isolated networks using renewable energy sources have been deployed for off-grid areas in Vietnam. However, the use of these energy sources in Vietnam’s isolated networks is still facing many challenges due to its infancy here. The issues of reliability and vulnerability of these networks are not given the expected attention. Another challenge is that the issues of the operational security of these systems could also be negatively affected by the variable nature of renewable sources, including static and dynamic security. For this reason, this study aims to contribute to a better understanding of integrating renewable energy into isolated networks, and in this case, using solar power for the An-Binh Island grid in Vietnam. The findings from this study suggest that choosing the right structure of the power mix could contribute to improving the operational security of isolated networks. Moreover, several solutions to enhance the reliability of this grid are also proposed. The NEPLAN environment was selected for simulation and analysis for all the scenarios in this study.
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- 2023
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42. The Protein Network in Subcutaneous Fat Biopsies from Patients with AL Amyloidosis: More Than Diagnosis?
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Dario Di Silvestre, Francesca Brambilla, Francesca Lavatelli, Maila Chirivì, Diana Canetti, Claudia Bearzi, Roberto Rizzi, Johan Bijzet, Bouke P. C. Hazenberg, Vittorio Bellotti, Julian D. Gillmore, and Pierluigi Mauri
- Subjects
amyloidosis ,proteomics ,systems biology ,networks ,PPI ,co-expression ,Cytology ,QH573-671 - Abstract
AL amyloidosis is caused by the misfolding of immunoglobulin light chains leading to an impaired function of tissues and organs in which they accumulate. Due to the paucity of -omics profiles from undissected samples, few studies have addressed amyloid-related damage system wide. To fill this gap, we evaluated proteome changes in the abdominal subcutaneous adipose tissue of patients affected by the AL isotypes κ and λ. Through our retrospective analysis based on graph theory, we have herein deduced new insights representing a step forward from the pioneering proteomic investigations previously published by our group. ECM/cytoskeleton, oxidative stress and proteostasis were confirmed as leading processes. In this scenario, some proteins, including glutathione peroxidase 1 (GPX1), tubulins and the TRiC complex, were classified as biologically and topologically relevant. These and other results overlap with those already reported for other amyloidoses, supporting the hypothesis that amyloidogenic proteins could induce similar mechanisms independently of the main fibril precursor and of the target tissues/organs. Of course, further studies based on larger patient cohorts and different tissues/organs will be essential, which would be a key point that would allow for a more robust selection of the main molecular players and a more accurate correlation with clinical aspects.
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- 2023
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43. Plasma exosomes characterization reveals a perioperative protein signature in older patients undergoing different types of on-pump cardiac surgery
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Carrozzo, Alessandro, Casieri, Valentina, Di Silvestre, Dario, Brambilla, Francesca, De Nitto, Emanuele, Sardaro, Nicola, Papini, Gaia, Storti, Simona, Settanni, Giuseppina, Solinas, Marco, Mauri, Pierluigi, Paparella, Domenico, and Lionetti, Vincenzo
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- 2021
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44. Repeated double cross-validation applied to the PCA-LDA classification of SERS spectra: a case study with serum samples from hepatocellular carcinoma patients
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Gurian, Elisa, Di Silvestre, Alessia, Mitri, Elisa, Pascut, Devis, Tiribelli, Claudio, Giuffrè, Mauro, Crocè, Lory Saveria, Sergo, Valter, and Bonifacio, Alois
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- 2021
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45. Proteomic Characterization of a 3D HER2+ Breast Cancer Model Reveals the Role of Mitochondrial Complex I in Acquired Resistance to Trastuzumab.
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Tapia, Ivana J., Perico, Davide, Wolos, Virginia J., Villaverde, Marcela S., Abrigo, Marianela, Di Silvestre, Dario, Mauri, Pierluigi, De Palma, Antonella, and Fiszman, Gabriel L.
- Subjects
HER2 positive breast cancer ,PROTEOMICS ,KREBS cycle ,LIQUID chromatography-mass spectrometry ,METABOLIC regulation ,TRASTUZUMAB - Abstract
HER2-targeted therapies, such as Trastuzumab (Tz), have significantly improved the clinical outcomes for patients with HER2+ breast cancer (BC). However, treatment resistance remains a major obstacle. To elucidate functional and metabolic changes associated with acquired resistance, we characterized protein profiles of BC Tz-responder spheroids (RSs) and non-responder spheroids (nRSs) by a proteomic approach. Three-dimensional cultures were generated from the HER2+ human mammary adenocarcinoma cell line BT-474 and a derived resistant cell line. Before and after a 15-day Tz treatment, samples of each condition were collected and analyzed by liquid chromatography–mass spectrometry. The analysis of differentially expressed proteins exhibited the deregulation of energetic metabolism and mitochondrial pathways. A down-regulation of carbohydrate metabolism and up-regulation of mitochondria organization proteins, the tricarboxylic acid cycle, and oxidative phosphorylation, were observed in nRSs. Of note, Complex I-related proteins were increased in this condition and the inhibition by metformin highlighted that their activity is necessary for nRS survival. Furthermore, a correlation analysis showed that overexpression of Complex I proteins NDUFA10 and NDUFS2 was associated with high clinical risk and worse survival for HER2+ BC patients. In conclusion, the non-responder phenotype identified here provides a signature of proteins and related pathways that could lead to therapeutic biomarker investigation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
46. Electrifying the Road to Net-Zero: Implications of Electric Vehicles and Carbon Emission Coefficient Factors in European Power Systems.
- Author
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Ali, Qais, Di Silvestre, Maria Luisa, Lombardi, Pio Alessandro, Riva Sanseverino, Eleonora, and Zizzo, Gaetano
- Abstract
The global trend is shifting towards adopting low-carbon transportation solutions, with electrification emerging as a prominent approach. The effectiveness of this transition in mitigating climate change hinges significantly on the source of electricity used for charging electric vehicles. This study focuses on four European Union countries: Switzerland, Italy, Germany, and Poland, each characterized by varying levels of carbon emissions from their power systems. Assumptions are made for the short-term (10%), medium-term (30%), and long-term (60%) penetration of electric vehicles, aligning with the 2050 net zero emissions targets. The study investigates the impact of these penetration levels on energy demand, exploring scenarios ranging from 100% renewable source-generated electricity to 100% coal-generated electricity for EV charging. Finally, utilizing PSS
® E 35.5 simulation software, the study assesses the implications of the electric vehicles' load on medium-voltage transmission lines. The findings highlight the substantial influence of electrifying the transport sector on both environmental sustainability and the power system infrastructure, underscoring the critical role of regional energy mixes and the power system carbon emissions coefficient factor. Regions with lower carbon emission coefficient factors witness significant benefits even with a modest transition to electric vehicles, whereas regions with high carbon emission coefficient factors experience minimal impact despite large-scale EV adoption. Additionally, densely populated urban areas may encounter challenges related to transmission line congestion to meet the growing demand for electric vehicle charging. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
47. Editorial: A Systems View of Plant Cellular Communication
- Author
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Dario Di Silvestre, Luca Tadini, Andrea Trotta, Luis Valledor, Ghasem Hosseini Salekdeh, and Jesus V. Jorrin Novo
- Subjects
systems biology ,plant communication ,OMICS data ,network analysis ,modeling ,Plant culture ,SB1-1110 - Published
- 2022
- Full Text
- View/download PDF
48. Circulating extracellular vesicles as non-invasive biomarker of rejection in heart transplant
- Author
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Castellani, Chiara, Burrello, Jacopo, Fedrigo, Marny, Burrello, Alessio, Bolis, Sara, Di Silvestre, Dario, Tona, Francesco, Bottio, Tomaso, Biemmi, Vanessa, Toscano, Giuseppe, Gerosa, Gino, Thiene, Gaetano, Basso, Cristina, Longnus, Sarah L., Vassalli, Giuseppe, Angelini, Annalisa, and Barile, Lucio
- Published
- 2020
- Full Text
- View/download PDF
49. Blockchain for power systems: Current trends and future applications
- Author
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Di Silvestre, Maria Luisa, Gallo, Pierluigi, Guerrero, Josep M., Musca, Rossano, Riva Sanseverino, Eleonora, Sciumè, Giuseppe, Vásquez, Juan C., and Zizzo, Gaetano
- Published
- 2020
- Full Text
- View/download PDF
50. Plasma Galectin-3 and urine proteomics predict FEV1 improvement in omalizumab-treated patients with severe allergic asthma: Results from the PROXIMA sub-study
- Author
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Riccio, Anna Maria, Mauri, Pierluigi, De Ferrari, Laura, Rossi, Rossana, Di Silvestre, Dario, Bartezaghi, Marta, Saccheri, Fabiana, and Canonica, Giorgio Walter
- Published
- 2020
- Full Text
- View/download PDF
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