7 results on '"Di Cristofaro, Sara"'
Search Results
2. Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation
- Author
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Franchini, Valeria, primary, Regalbuto, Elisa, additional, De Amicis, Andrea, additional, De Sanctis, Stefania, additional, Di Cristofaro, Sara, additional, Coluzzi, Elisa, additional, Marinaccio, Jessica, additional, Sgura, Antonella, additional, Ceccuzzi, Silvio, additional, Doria, Andrea, additional, Gallerano, Gian Piero, additional, Giovenale, Emilio, additional, Ravera, Gian Luca, additional, Bei, Roberto, additional, Benvenuto, Monica, additional, Modesti, Andrea, additional, Masuelli, Laura, additional, and Lista, Florigio, additional
- Published
- 2018
- Full Text
- View/download PDF
3. Study of the effects of 0.15 terahertz radiation on genome integrity of adult fibroblasts
- Author
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Franchini, Valeria, primary, De Sanctis, Stefania, additional, Marinaccio, Jessica, additional, De Amicis, Andrea, additional, Coluzzi, Elisa, additional, Di Cristofaro, Sara, additional, Lista, Florigio, additional, Regalbuto, Elisa, additional, Doria, Andrea, additional, Giovenale, Emilio, additional, Gallerano, Gian Piero, additional, Bei, Roberto, additional, Benvenuto, Monica, additional, Masuelli, Laura, additional, Udroiu, Ion, additional, and Sgura, Antonella, additional
- Published
- 2018
- Full Text
- View/download PDF
4. Dose Estimation Using Dicentric Chromosome Assay and Cytokinesis Block Micronucleus Assay
- Author
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De Amicis, Andrea, primary, De Sanctis, Stefania, additional, Di Cristofaro, Sara, additional, Franchini, Valeria, additional, Regalbuto, Elisa, additional, Mammana, Giacomo, additional, and Lista, Florigio, additional
- Published
- 2014
- Full Text
- View/download PDF
5. Study of the effects of 0.15 terahertz radiation on genome integrity of adult fibroblasts
- Author
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Valeria, Franchini, Stefania, De Sanctis, Jessica, Marinaccio, Andrea, De Amicis, Elisa, Coluzzi, Sara, Di Cristofaro, Florigio, Lista, Elisa, Regalbuto, Andrea, Doria, Emilio, Giovenale, Gian Piero, Gallerano, Roberto, Bei, Monica, Benvenuto, Laura, Masuelli, Ion, Udroiu, Antonella, Sgura, Franchini, Valeria, DE SANCTIS, Stefania, Marinaccio, Jessica, DE AMICIS, Andrea, Coluzzi, Elisa, Di Cristofaro, Sara, Lista, Florigio, Regalbuto, Elisa, Doria, Andrea, Giovenale, Emilio, Gallerano, Gian Piero, Bei, Roberto, Benvenuto, Monica, Masuelli, Laura, Udroiu, Ion, and Sgura, Antonella
- Subjects
Adult ,Chromosome Aberrations ,Micronucleus Tests ,human fibroblasts ,Cell Cycle ,Telomere Homeostasis ,Fibroblasts ,Chromosomal malsegregation ,DNA damage ,Human fibroblasts ,Spindle disturbance ,THz radiation ,Epidemiology ,Genetics (clinical) ,Health, Toxicology and Mutagenesis ,Aneuploidy ,Settore MED/04 ,Genomic Instability ,Cell Line ,spindle disturbance ,human fibroblast ,chromosomal malsegregation ,Humans ,Terahertz Radiation - Abstract
The applications of Terahertz (THz) technologies have significantly developed in recent years, and the complete understanding of the biological effects of exposure to THz radiation is becoming increasingly important. In a previous study, we found that THz radiation induced genomic damage in fetal fibroblasts. Although these cells demonstrated to be a useful model, exposure of human foetuses to THz radiation is highly improbable. Conversely, THz irradiation of adult dermal tissues is cause of possible concern for some professional and nonprofessional categories. Therefore, we extended our study to the investigation of the effects of THz radiation on adult fibroblasts (HDF). In this work, the effects of THz exposure on HDF cells genome integrity, cell cycle, cytological ultrastructure and proteins expression were assessed. Results of centromere-negative micronuclei frequencies, phosphorylation of H2AX histone, and telomere length modulation indicated no induction of DNA damage. Concordantly, no changes in the expression of proteins associated with DNA damage sensing and repair were detected. Conversely, our results showed an increase of centromere-positive micronuclei frequencies and chromosomal nondisjunction events, indicating induction of aneuploidy. Therefore, our results indicate that THz radiation exposure may affect genome integrity through aneugenic effects, and not by DNA breakage. Our findings are compared to published studies, and possible biophysical mechanisms are discussed. Environ. Mol. Mutagen. 59:476-487, 2018. © 2018 Wiley Periodicals, Inc.
- Published
- 2018
6. Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation
- Author
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Elisa Regalbuto, Antonella Sgura, Jessica Marinaccio, Valeria Franchini, Laura Masuelli, Andrea De Amicis, Gian Luca Ravera, Florigio Lista, Gian Piero Gallerano, Elisa Coluzzi, Emilio Giovenale, Silvio Ceccuzzi, Monica Benvenuto, Stefania De Sanctis, Roberto Bei, Andrea Modesti, Sara Di Cristofaro, Andrea Doria, Franchini, Valeria, Regalbuto, Elisa, De Amicis, Andrea, De Sanctis, Stefania, Di Cristofaro, Sara, Coluzzi, Elisa, Marinaccio, Jessica, Sgura, Antonella, Ceccuzzi, Silvio, Doria, Andrea, Gallerano, Gian Piero, Giovenale, Emilio, Ravera, Gian Luca, Bei, Roberto, Benvenuto, Monica, Modesti, Andrea, Masuelli, Laura, and Lista, Florigio
- Subjects
0301 basic medicine ,Radiology, Nuclear Medicine and Imaging ,Epidemiology ,Health, Toxicology and Mutagenesis ,Aneuploidy ,Micronuclei ,Histones ,Direct DNA damage ,Nuclear Medicine and Imaging ,Fetu ,Cells, Cultured ,Cultured ,Micronucleus Tests ,Histone ,Health ,Micronucleus test ,Fibroblast ,electromagnetic fields ,genetic effects, radiation ,microwaves ,radiation, nonionizing ,genetic effects ,Comet Assay ,Radiology ,Human ,Adult ,microwave ,DNA damage ,Cells ,Biology ,03 medical and health sciences ,Fetus ,electromagnetic field ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Toxicology and Mutagenesis ,Micronuclei, Chromosome-Defective ,Settore MED/04 - Patologia Generale ,DNA Damage ,Fibroblasts ,Microwaves ,medicine.disease ,Comet assay ,radiation ,030104 developmental biology ,nonionizing ,Cell culture ,Apoptosis ,Cancer research ,biology.protein ,Micronucleus Test ,Chromosome-Defective - Abstract
In the last decades, technological development has led to an increasing use of devices and systems based on microwave radiation. The increased employment of these devices has elicited questions about the potential long-term health consequences associated with microwave radiation exposure. From this perspective, biological effects of microwave radiation have been the focus of many studies, but the reported scientific data are unclear and contradictory. The aim of this study is to evaluate the potential genotoxic and cellular effects associated with in vitro exposure of human fetal and adult fibroblasts to microwave radiation at the frequency of 25 GHz. For this purpose, several genetic and biological end points were evaluated. Results obtained from comet assay, phosphorylation of H2AX histone, and antikinetochore antibody (CREST)-negative micronuclei frequency excluded direct DNA damage to human fetal and adult fibroblasts exposed to microwaves. No induction of apoptosis or changes in prosurvival signalling proteins were detected. Moreover, CREST analysis showed for both the cell lines an increase in the total number of micronuclei and centromere positive micronuclei in exposed samples, indicating aneuploidy induction due to chromosome loss.
- Published
- 2018
7. Biological Effects of 25 to 150 GHz Radiation After In Vitro Exposure of Human Fibroblasts: a Comparison of Experimental Results
- Author
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Laura Masuelli, Gian Piero Gallerano, Elisa Coluzzi, Roberto Bei, Andrea Modesti, Gian Luca Ravera, Jessica Marinaccio, Valeria Franchini, Stefania De Sanctis, Elisa Regalbuto, Antonella Sgura, Silvio Ceccuzzi, Andrea De Amicis, Monica Benvenuto, Emilio Giovenale, Florigio Lista, Sara Di Cristofaro, Andrea Doria, Franchini, Valeria, Ceccuzzi, Silvio, Doria, Andrea, Gallerano, Gian Piero, Giovenale, Emilio, Ravera, Gian Luca, De Amicis, Andrea, De Sanctis, Stefania, Di Cristofaro, Sara, Regalbuto, Elisa, Coluzzi, Elisa, Marinaccio, Jessica, Sgura, Antonella, Bei, Roberto, Benvenuto, Monica, Modesti, Andrea, Masuelli, Laura, and Lista, Florigio
- Subjects
0301 basic medicine ,Genome integrity ,Terahertz ,Biological effects ,Millimeter waves ,Condensed Matter Physic ,Radiation ,03 medical and health sciences ,0302 clinical medicine ,Fibroblasts ,Human primary cells ,In vitro exposure ,Non-ionizing radiation ,Instrumentation ,Condensed Matter Physics ,Electrical and Electronic Engineering ,Human primary cell ,Biological effect ,Pulsed radiation ,Settore MED/04 - Patologia Generale ,Chemistry ,Millimeter wave ,Cell cycle ,Cell biology ,030104 developmental biology ,030220 oncology & carcinogenesis ,Human fetal ,Ultrastructure ,Fibroblast - Abstract
In this paper, we present a comprehensive discussion of the results obtained after in vitro exposure of human fetal fibroblasts and human adult fibroblasts to pulsed radiation in a wide band between 100 and 150 GHz and to continuous wave radiation at 25 GHz. In order to assess potential effects of exposure, the genome integrity, cell cycle, cytological ultrastructure, and proteins expression were evaluated.
- Published
- 2018
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