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This study aims to determine the effect of the preparation method and the number of samples on the quality of purified DNA from beef sausage. The study was conducted using a Randomized Block Design. The main factor is the sample preparation method which consists of P1: fresh sample; P2: oven-dried sample; and P3: Freeze-drying dry samples. Each treatment consisted of 4 groups, namely K1: sample weight 25 mg; K2: sample weight 50 mg; K3: sample weight 75 mg; and K4: sample weight 100 mg. Each treatment group was repeated 2 times so that the total sample was 24. The results showed that DNA purification using different preparation methods and sample weights was successfully carried out, as seen from the presence of DNA bands visualized on agarose gel with EtBr staining. The average DNA quality of the P1, P2, and P3 preparation methods were 1.60±0.90 ng/μl, 2.63±0.99 ng/μl, and 2.94 ±0.89, ng/μl respectively, with DNA purity 1.023 ±0.165, 0.937±0.148, and 1.014±0.163. The average DNA quality at K1, K2, K3, and K4 obtained DNA concentrations of 3.03±1.64 ng/μl, 3.15±0.74 ng/μl, 2.28± 1.66 ng/μl, and 2.54±1.50 ng/μl with a purity of 1.059±0.142, 0.981±0.130, 0.908±0.061, and 1.061± 0.215. The average total concentration of purified DNA from beef sausage was 2.75±1.28 ng/μl with a purity of 0.991±0.149. The results of variance showed that the treatment did not affect the concentration and purity of purified DNA from beef sausage. This study concludes that DNA purification from beef sausage can be carried out, but the preparation method and number of samples do not affect the quality and quantity of DNA.

Tujuan penelitian adalah untuk menganalisis motivasi peternak bagi hasil usaha ternak sapi potong dan faktor-faktor yang mempengaruhi motivasi peternak usaha ternak sapi potong dengan pola sistem bagi hasil. Waktu pengambilan data mulai bulan januari sampai dengan bulan februari 2017. Penelitian dilakukan di Kecamatan Maiwa Kabupaten Enrekang, Metode Penelitian survey. Sampel berjumlah 64 peternak diambil (total sampling). Metode analisis data yaitu pengujian model statistik menggunakan SEM SmartPLS (Partial Least Square) 2.0. Hasil penelitian menjelaskan bahwa 1. Motivasi peternak sistem bagi hasil usaha sapi potong yaitu memenuhi kebutuhan hidup keluarga, memenuhi kebutuhan sekolah anak-anak, memenuhi kebutuhan sekunder, hubungan dengan pemilik modal, hubungan dengan penyuluh, hubungan dengan pemerintah, hubungan dengan masyarakat, hubungan dengan peternak lain, memperoleh penghargaan dari sistem bagi hasil, serta memperoleh pengetahuan dan pengalaman beternak. 2. Faktor-faktor yang mempengaruhi motivasi peternak sistem bagi hasil usaha sapi potong yaitu jumlah ternak yang dimiliki, pendapatan non peternak, pendapatan sistem bagi hasil, penguasaan lahan pertanian, dan jumlah pedet yang dihasilkan.

Salah satu gangguan reproduksi yang banyak terjadi di Peternakan sapi perah, yaitu kawin berulang (Repeat Breeding). Tujuan penelitian ini adalah untuk melihat efisiensi reproduksi induk sapi perah yang mengalami kawin berulang. Penelitian ini dilakukan secara survei dengan pengumpulan data primer dan sekunder. Pengambilan data primer dilakukan dengan cara pengamatan, melihat recording dan wawancara langsung dengan peternak menggunakan daftar pertanyaan yang tersedia, sedangkan data sekunder diperoleh dari petugas inseminator di Dusun Panette Kecamatan Cendana Kabupaten Enrekang Sulawesi Selatan. Hasil penelitian menunjukkan bahwa pada ternak normal rata-rata calving interval adalah 13 bulan sedangkan pada ternak yang mengalami kawin berulang rata-rata calving interval adalah 24 bulan. Sapi perah dengan calving interval yang panjang menunjukkan bahwa sapi perah tersebut mempunyai efisiensi reproduksi yang rendah. Sebaliknya, sapi perah betina dengan calving interval yang pendek menunjukkan bahwa sapi perah tersebut memiliki efisiensi reproduksi yang tinggi. Kesimpulan penelitian ini adalah efisiensi reproduksi ternak yang mengalami kawin berulang jika dilihat dari calving interval yaitu lebih panjang dibandingkan dengan ternak dengan kondisi normal. Sapi perah dengan calving interval yang panjang menunjukkan bahwa sapi perah tersebut mempunyai efisiensi reproduksi yang rendah.

The aim of this study was to know the incidence of repeat breeding in dairy cows and to know the effectiveness of Heatsynch protocol on dairy repeat breeding cows. The study was conducted in two stages. The first stage, all dairy cows were recorded and clinically examined through palpation per rectum and for their reproductive statuses. Parameter measured in the study was the incidence of repeat breeding. The second stage of the study was that the repeat breeder cows subjected to Heatsynch protocol for estrus induction. The protocol was GnRH injection on day-0, followed by PGF2α, estradiol injections on day-7 and day-8, respectively, and inseminated artificially on day-9. The results of the study indicated that the incidence of repeat breeding was 41.6%. The Heatsynch protocol was successfully increase the number of pregnant repeat breeder cows; 75%, higher compared to untreated repeat breeder cows; 20%. It can be concluded that high incidence of repeat breeding in dairy cows. Heatsynch protocol can be used to treat repeat breeder cows and improve the reproductive efficiency.

The aim of this study was to determine the incidence of abnormalities of reproductive organs, and to determine the effectiveness of Heatsynchprotocol in dairy cows that suffered from abnormalities of reproductive organs. This study was conducted in smallholder dairy farms in Panette, Cendana Sub-district, Enrekang Regency and the study was conducted in two stages. The first stage; a total of 77 dairy cows were used to determine the incidence of reproductive organs abnormalities. The second stage; a total of 20 dairy cows that suffered from reproductive organs abnormalities were divided into two groups. The first 10 dairy cows were treated with Heatsynch, and the other 10 dairy cows were used as a negative control (without Heatsynch treatment). For positive control, 10 dairy cows that did not suffered from abnormalities of reproduction organs were used. The results of this study showed that the incidence of reproductive organs abnormalities was 36.4% and the highest of abnormalities was uterine disorders at 26%. The percentage of cows suffered from reproductive organs abnormalities that became pregnant after treated with Heatsynch was 60%, while none cows become pregnant without treatment. The average interval from calving to pregnancy in Heatsynch group was 512 days, and in control negative cows, none become pregnant up to 557 days after calving. It can be concluded that the incidence of abnormalities of reproductive organs in dairy cattle was still high. The Heatsynchprotocol can improve the abnormalities of the reproductive organs in the dairy cows and improve reproductive efficiency by increase the pregnancy rate and shortening calving interval.

The aim of this study was to find out the application of Heatsynch protocol in overcome the delayed puberty of dairy heifers that suffered from infertility. This study was conducted at small dairy farms in Sub-district of Cendana, Enrekang Regency, Indonesia. The study was conducted in two stages. In the first stage, a total of 50 dairy heifers were used to determine the percentage of heifers that experienced delay in puberty. In the second stage, 20 dairy heifers with delayed puberty were divided into two groups. The first group, 10 dairy heifers were treated with Heatsynch and the other 10 dairy heifers were used as negative control (without Heatsynch). For control positive, 10 dairy heifers that did not suffered from delayed puberty were used. In Heatsynch treated dairy heifers, GnRH were injected on day-0, followed by PGF2α, estradiol injections on day-7 and day-8, respectively, and inseminated artificially on day-9. The results of this study showed that the percentage of dairy heifers that experienced in delayed puberty was 44%. The mean age at first pregnant after treated with Heatsynch was 545 days, shorter than those heifers in control negative (644 days). The percentage of dairy heifers become pregnant after treating with Heatsynch was 80%, higher than those untreated delayed puberty heifers (50%). It can be concluded that the incidence of delayed puberty in dairy heifers was still high. Heatsynch protocol can be one way that can be used to overcome the problem of delayed puberty in dairy heifers and to improve reproductive efficiency.