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16. Prophylaxis of implant-related infections by local release of vancomycin from a hydrogel in rabbits

Author

Orthopaedie Onderzoek, Other research (not in main researchprogram), MS Orthopaedie Algemeen, Infection & Immunity, Pathologie Pathologen staf, Cancer, MMB Medische Staf, MKA/BT Onderzoek, Regenerative Medicine and Stem Cells, Boot, W, Vogely, H C, Nikkels, P G, Pouran, B, van Rijen, M H, Ekkelenkamp, M B, Hänsch, G M, Dhert, W J, Gawlitta, D, Orthopaedie Onderzoek, Other research (not in main researchprogram), MS Orthopaedie Algemeen, Infection & Immunity, Pathologie Pathologen staf, Cancer, MMB Medische Staf, MKA/BT Onderzoek, Regenerative Medicine and Stem Cells, Boot, W, Vogely, H C, Nikkels, P G, Pouran, B, van Rijen, M H, Ekkelenkamp, M B, Hänsch, G M, Dhert, W J, and Gawlitta, D

Published
2020

20. LOCAL INDUCTION OF INFLAMMATION AFFECTS BONE FORMATION

Author

Croes, M, Kruyt, M C, Loozen, L, Kragten, A H, Yuan, H, Dhert, W J, Öner, F C, Alblas, J, Faculteit Diergeneeskunde, Regenerative Medicine, Stem Cells & Cancer, dES RMSC, Division Instructive Biomaterials Eng, RS: MERLN - Complex Tissue Regeneration (CTR), RS: MERLN - Instructive Biomaterials Engineering (IBE), Faculteit Diergeneeskunde, Regenerative Medicine, Stem Cells & Cancer, and dES RMSC

Subjects
0301 basic medicine, Calcium Phosphates, Lipopolysaccharides, Male, Pathology, OSTEOGENIC DIFFERENTIATION, lcsh:Diseases of the musculoskeletal system, Osteoimmunology, Bone Morphogenetic Protein 2, Rabbit, ILIAC CREST AUTOGRAFT, Biochemistry, lipoteichoicacid, tumour necrosis factor alpha, Subcutaneous Tissue, Implants, Experimental, bone regeneration, Osteogenesis, bone morphogenetic protein, Lymphocytes, GROWTH-FACTORS, Cell Aggregation, Chemistry, lipopolysaccharide, Bone cement, TNF-ALPHA, Cell biology, Bone regeneration, Bone morphogenetic protein 7, lipoteichoic acid, Lipoteichoic acid, Rabbits, medicine.symptom, medicine.medical_specialty, lcsh:Surgery, Biomedical Engineering, rabbit, Inflammation, Lipopolysaccharide, Bioengineering, Bone morphogenetic protein, MESENCHYMAL STEM-CELLS, Tumour necrosis factor alpha, Biomaterials, 03 medical and health sciences, TISSUE REGENERATION, In vivo, medicine, Journal Article, Animals, INDUCED MEMBRANES, HETEROTOPIC OSSIFICATION, Fluorescent Dyes, osteoimmunology, TOLL-LIKE RECEPTORS, NECROSIS-FACTOR-ALPHA, lcsh:RD1-811, Cell Biology, Rats, 030104 developmental biology, inflammation, tumournecrosis factor alpha, lcsh:RC925-935
Abstract

To explore the influence of inflammatory processes on bone formation, we applied a new in vivo screening model. Confined biological pockets were first created in rabbits as a response to implanted bone cement discs. These biomembrane pockets were subsequently used to study the effects of inflammatory stimuli on ectopic bone formation within biphasic calcium phosphate (BCP) constructs loaded with TNF-alpha, lipopolysaccharide (LPS) or lipoteichoic acid (LTA), all with or without bone morphogenetic protein (BMP)-2. Analysis of bone formation after 12 weeks demonstrated that the inflammatory mediators were not bone-inductive in combination with the BCP alone, but inhibited or enhanced BMP-induced bone formation. LPS was associated with a strong inhibition of bone formation by BMP-2, while LTA and TNF-alpha showed a positive interaction with BMP-2. Since the biomembrane pockets did not interfere with bone formation and prevented the leakage of pro-inflammatory compounds to the surrounding tissue, the biomembrane model can be used for in vivo approaches to study local inflammation in conjunction with new bone formation. Using this model, it was shown that the modulation of the inflammatory response could be beneficial or detrimental to the subsequent bone formation process. The co-delivery of inflammatory factors and bone-related growth factors should be further explored as a strategy to enhance the bone-forming efficacy of bone substitutes.

Published
2017
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22. The role of bacterial stimuli in inflammation-driven bone formation

Author

Croes, M., Kruyt, M. C., Boot, W., Pouran, B., Braham, M. V., Pakpahan, S. A., Weinans, H., Vogely, H. C., Fluit, A. C., Dhert, W. J., Alblas, J., Öner, F. C., Croes, M., Kruyt, M. C., Boot, W., Pouran, B., Braham, M. V., Pakpahan, S. A., Weinans, H., Vogely, H. C., Fluit, A. C., Dhert, W. J., Alblas, J., and Öner, F. C.

Published
2019

23. The role of bacterial stimuli in inflammation-driven bone formation

Author

Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, Infection & Immunity, MMB Zorg, MS Orthopaedie Spine, Croes, M., Kruyt, M. C., Boot, W., Pouran, B., Braham, M. V., Pakpahan, S. A., Weinans, H., Vogely, H. C., Fluit, A. C., Dhert, W. J., Alblas, J., Öner, F. C., Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, Infection & Immunity, MMB Zorg, MS Orthopaedie Spine, Croes, M., Kruyt, M. C., Boot, W., Pouran, B., Braham, M. V., Pakpahan, S. A., Weinans, H., Vogely, H. C., Fluit, A. C., Dhert, W. J., Alblas, J., and Öner, F. C.

Published
2019

25. Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

Author

Vonk, L A, de Windt, T S, Kragten, A H M, Beekhuizen, M, Mastbergen, S C, Dhert, W J A, Lafeber, F P J G, Creemers, L B, Saris, D B F, Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, CSCA TR1, Tissue Repair, Faculty of Science and Technology, Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, CSCA TR1, and Tissue Repair

Subjects
Cartilage, Articular, musculoskeletal diseases, Pathology, medicine.medical_specialty, Bone Regeneration, Knee Joint, Biomedical Engineering, Cell- and Tissue-Based Therapy, Osteoarthritis, Matrix (biology), Cell therapy, Chondrons, Chondrocytes, Cartilage repair, Rheumatology, Articular cartilage repair, medicine, Animals, Orthopedics and Sports Medicine, Autologous chondrocyte implantation, Cells, Cultured, biology, Regeneration (biology), Cartilage, Goats, Anatomy, Osteoarthritis, Knee, medicine.disease, Disease Models, Animal, medicine.anatomical_structure, Proteoglycan, biology.protein, Female
Abstract

OBJECTIVE: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. METHODS: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. RESULTS: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P < 0.01). Besides, chondrons still reflected whether they were isolated from a damaged joint, even if they where isolated from the opposing or adjacent condyle. Although chondrocytes did not reflect this diseased status of the joint, chondrons always outperformed chondrocytes, even when isolated from the damaged joints (P < 0.0001). Besides increased cartilage production, the chondrons showed less collagenase activity compared to the chondrocytes. CONCLUSION: Chondrons still outperform chondrocytes when they were isolated from a damaged joint and they might be a superior cell source for articular cartilage repair and cell-induced cartilage formation.

Published
2014
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26. Hyaluronic Acid-Based Hydrogel Coating Does Not Affect Bone Apposition at the Implant Surface in a Rabbit Model

Author

Boot, W, Gawlitta, D, Nikkels, P G J, Pouran, B, van Rijen, M H P, Dhert, W J A, Vogely, H Ch, Boot, W, Gawlitta, D, Nikkels, P G J, Pouran, B, van Rijen, M H P, Dhert, W J A, and Vogely, H Ch

Abstract

BACKGROUND: Uncemented orthopaedic implants rely on the bone-implant interface to provide stability, therefore it is essential that a coating does not interfere with the bone-forming processes occurring at the implant interface. In addition, local application of high concentrations of antibiotics for prophylaxis or treatment of infection may be toxic for osteoblasts and could impair bone growth.QUESTIONS/PURPOSES: In this animal study, we investigated the effect of a commercially available hydrogel, either unloaded or loaded with 2% vancomycin. We asked, does unloaded hydrogel or hydrogel with vancomycin (1) interfere with bone apposition and timing of bone deposition near the implant surface; and (2) induce a local or systemic inflammatory reaction as determined by inflammation around the implant and hematologic parameters.METHODS: In 18 New Zealand White rabbits, an uncoated titanium rod (n = 6), a rod coated with unloaded hydrogel (n = 6), or a rod coated with 2% vancomycin-loaded hydrogel (n = 6) was implanted in the intramedullary canal of the left tibia. After 28 days, the bone volume fraction near the implant was measured with microCT analysis, inflammation was semiquantitatively scored on histologic sections, and timing of bone apposition was followed by semiquantitative scoring of fluorochrome incorporation on histologic sections. Two observers, blinded to the treatment, scored the sections and reconciled their scores if there was a disagreement. The hematologic inflammatory reaction was analyzed by measuring total and differential leukocyte counts and erythrocyte sedimentation rates in blood. With group sizes of six animals per group, we had 79% power to detect a difference of 25% in histologic scoring for infection and inflammation.RESULTS: No differences were found in the amount of bone apposition near the implant in the No Gel group (48.65% ± 14.95%) compared with the Gel group (59.97% ± 5.02%; mean difference [MD], 11.32%; 95% C

Published
2017

27. Local induction of inflammation affects bone formation

Author

Croes, M., Kruyt, M. C., Loozen, L., Kragten, A. H M, Yuan, H., Dhert, W. J., Öner, F. C., Alblas, Jacqueline, Croes, M., Kruyt, M. C., Loozen, L., Kragten, A. H M, Yuan, H., Dhert, W. J., Öner, F. C., and Alblas, Jacqueline

Published
2017

28. Local induction of inflammation affects bone formation

Author

Faculteit Diergeneeskunde, Regenerative Medicine, Stem Cells & Cancer, dES RMSC, Croes, M, Kruyt, M C, Loozen, L, Kragten, A H, Yuan, H, Dhert, W J, Öner, F C, Alblas, J, Faculteit Diergeneeskunde, Regenerative Medicine, Stem Cells & Cancer, dES RMSC, Croes, M, Kruyt, M C, Loozen, L, Kragten, A H, Yuan, H, Dhert, W J, Öner, F C, and Alblas, J

Published
2017

30. Hyaluronic Acid-Based Hydrogel Coating Does Not Affect Bone Apposition at the Implant Surface in a Rabbit Model

Author

Zorgeenheid Orthopaedie Zorg, Other research (not in main researchprogram), MKA/BT Onderzoek, Regenerative Medicine and Stem Cells, Pathologie patiënten zorg, Cancer, Infection & Immunity, Boot, W., Gawlitta, Debby, Nikkels, P. G J, Pouran, B., van Rijen, M. H P, Dhert, W. J A, Vogely, H. C., Zorgeenheid Orthopaedie Zorg, Other research (not in main researchprogram), MKA/BT Onderzoek, Regenerative Medicine and Stem Cells, Pathologie patiënten zorg, Cancer, Infection & Immunity, Boot, W., Gawlitta, Debby, Nikkels, P. G J, Pouran, B., van Rijen, M. H P, Dhert, W. J A, and Vogely, H. C.

Published
2017

31. Local induction of inflammation affects bone formation

Author

Zorgeenheid Orthopaedie Zorg, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Croes, M., Kruyt, M. C., Loozen, L., Kragten, A. H M, Yuan, H., Dhert, W. J., Öner, F. C., Alblas, Jacqueline, Zorgeenheid Orthopaedie Zorg, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Croes, M., Kruyt, M. C., Loozen, L., Kragten, A. H M, Yuan, H., Dhert, W. J., Öner, F. C., and Alblas, Jacqueline

Published
2017

32. Comparative study of depth-dependent characteristics of equine and human osteochondral tissue from the medial and lateral femoral condyles

Author

Malda, J, Benders, K E M, Klein, T J, de Grauw, J C, Kik, M J L, Hutmacher, D W, Saris, D B F, van Weeren, P R, Dhert, W J A, Dep Gezondheidszorg Paard, LS Equine Muscoskeletal Biology, Afd Veterinaire Anesthesiologie, Veterinair Pathologisch Diagnostisch Cnt, Faculteit Diergeneeskunde, Onderzoek, ES TR, Dep Gezondheidszorg Paard, LS Equine Muscoskeletal Biology, Afd Veterinaire Anesthesiologie, Veterinair Pathologisch Diagnostisch Cnt, Faculteit Diergeneeskunde, Onderzoek, and ES TR

Subjects
Cartilage, Articular, 0206 medical engineering, Biomedical Engineering, 02 engineering and technology, Osteoarthritis, Glycosaminoglycan, 03 medical and health sciences, Rheumatology, Species Specificity, Models, In vivo, Medicine, Animals, Humans, Orthopedics and Sports Medicine, Animal model, Femur, Horses, Process (anatomy), 030304 developmental biology, Aged, Glycosaminoglycans, 0303 health sciences, Tissue Engineering, Animal, business.industry, Cartilage, FEMORAL CONDYLE, Histology, Anatomy, DNA, medicine.disease, 090300 BIOMEDICAL ENGINEERING, 020601 biomedical engineering, Zonal, medicine.anatomical_structure, International, Models, Animal, Joints, Implant, Collagen, business, Articular
Abstract

Articular cartilage defects are common after joint injuries. When left untreated, the biomechanical protective function of cartilage is gradually lost, making the joint more susceptible to further damage, causing progressive loss of joint function and eventually osteoarthritis (OA). In the process of translating promising tissue-engineering cartilage repair approaches from bench to bedside, pre-clinical animal models including mice, rabbits, goats, and horses, are widely used. The equine species is becoming an increasingly popular model for the in vivo evaluation of regenerative orthopaedic approaches. As there is also an increasing body of evidence suggesting that successful lasting tissue reconstruction requires an implant that mimics natural tissue organization, it is imperative that depth-dependent characteristics of equine osteochondral tissue are known, to assess to what extent they resemble those in humans. Therefore, osteochondral cores (4-8 mm) were obtained from the medial and lateral femoral condyles of equine and human donors. Cores were processed for histology and for biochemical quantification of DNA, glycosaminoglycan (GAG) and collagen content. Equine and human osteochondral tissues possess similar geometrical (thickness) and organizational (GAG, collagen and DNA distribution with depth) features. These comparable trends further underscore the validity of the equine model for the evaluation of regenerative approaches for articular cartilage.

Published
2012

33. Arthroscopic airbrush assisted cell implantation for cartilage repair in the knee: a controlled laboratory and human cadaveric study

Author

de Windt, T S, Vonk, L A, Buskermolen, J K, Visser, J., Karperien, M, Bleys, R L A W, Dhert, W J A, Saris, D B F, de Windt, T S, Vonk, L A, Buskermolen, J K, Visser, J., Karperien, M, Bleys, R L A W, Dhert, W J A, and Saris, D B F

Abstract

OBJECTIVE: The objective of this study was to investigate the feasibility of arthroscopic airbrush assisted cartilage repair.METHODS: An airbrush device (Baxter) was used to spray both human expanded osteoarthritic chondrocytes and choncrocytes with their pericellular matrix (chondrons) at 1 × 10(6) cells/ml fibrin glue (Tissucol, Baxter) in vitro. Depth-dependent cell viability was assessed for both methods with confocal microscopy. Constructs were cultured for 21 days to assess matrix production. A controlled human cadaveric study (n = 8) was performed to test the feasibility of the procedure in which defects were filled with either arthroscopic airbrushing or needle extrusion. All knees were subjected to 60 min of continuous passive motion and scored on outline attachment and defect filling.RESULTS: Spraying both chondrocytes and chondrons in fibrin glue resulted in a homogenous cell distribution throughout the scaffold. No difference in viability or matrix production between application methods was found nor between chondrons and chondrocytes. The cadaveric study revealed that airbrushing was highly feasible, and that defect filling through needle extrusion was more difficult to perform based on fibrin glue adhesion and gravity-induced seepage. Defect outline and coverage scores were consistently higher for extrusion, albeit not statistically significant.CONCLUSION: Both chondrons and chondrocytes can be evenly distributed in a sprayed fibrin glue scaffold without affecting viability while supporting matrix production. The airbrush technology is feasible, easier to perform than needle extrusion and allows for reproducible arthroscopic filling of cartilage defects.

Published
2015

34. Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation onset and volume

Author

Wegman, F.C.M. (author), Poldervaart, M. T. (author), van der Helm, Y. J. (author), Oner, F. C. (author), Dhert, W. J. (author), Alblas, J. (author), Wegman, F.C.M. (author), Poldervaart, M. T. (author), van der Helm, Y. J. (author), Oner, F. C. (author), Dhert, W. J. (author), and Alblas, J. (author)

Abstract

Bone morphogenetic protein-2 (BMP-2) gene delivery has shown to induce bone formation in vivo in cell-based tissue engineering. In addition, the chemoattractant stromal cell-derived factor-1α (SDF-1α, also known as CXCL12) is known to recruit multipotent stromal cells towards its release site where it enhances vascularisation and possibly contributes to osteogenic differentiation. To investigate potential cooperative behaviour for bone formation, we investigated combined release of BMP-2 and SDF-1α on ectopic bone formation in mice. Multipotent stromal cellseeded and cell-free constructs with BMP-2 plasmid DNA and /or SDF-1α loaded onto gelatin microparticles, were implanted subcutaneously in mice for a period of 6 weeks. Histological analysis and histomorphometry revealed that the onset of bone formation and the formed bone volume were both enhanced by the combination of BMP-2 and SDF-1α compared to controls in cell-seeded constructs. Samples without seeded multipotent stromal cells failed to induce any bone formation. We conclude that the addition of stromal cellderived factor-1α to a cell-seeded alginate based bone morphogenetic protein-2 plasmid DNA construct has an additive effect on bone formation and can be considered a promising combination for bone regeneration., Transport and Planning

Published
2015

37. Arthroscopic airbrush assisted cell implantation for cartilage repair in the knee: a controlled laboratory and human cadaveric study

Author

CSCA TR1, Regenerative Medicine, Stem Cells & Cancer, Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, de Windt, T S, Vonk, L A, Buskermolen, J K, Visser, J., Karperien, M, Bleys, R L A W, Dhert, W J A, Saris, D B F, CSCA TR1, Regenerative Medicine, Stem Cells & Cancer, Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, de Windt, T S, Vonk, L A, Buskermolen, J K, Visser, J., Karperien, M, Bleys, R L A W, Dhert, W J A, and Saris, D B F

Published
2015

39. Growth factor-induced osteogenesis in a novel radiolucent bone chamber

Author

dES RMSC, Poldervaart, M. T., van der Stok, J., de Haas, M., ‘T Hart, M. C., Öner, F. C., Dhert, W. J A, Weinans, H., Alblas, J., dES RMSC, Poldervaart, M. T., van der Stok, J., de Haas, M., ‘T Hart, M. C., Öner, F. C., Dhert, W. J A, Weinans, H., and Alblas, J.

Published
2015

40. Arthroscopic airbrush assisted cell implantation for cartilage repair in the knee: A controlled laboratory and human cadaveric study

Author

Orthopaedie Onderzoek, Orthopaedie Opleiding, Regenerative Medicine and Stem Cells, Anatomie, Other research (not in main researchprogram), de Windt, T. S., Vonk, L. A., Buskermolen, J. K., Visser, J., Karperien, M., Bleys, R. L A W, Dhert, W. J A, Saris, D. B F, Orthopaedie Onderzoek, Orthopaedie Opleiding, Regenerative Medicine and Stem Cells, Anatomie, Other research (not in main researchprogram), de Windt, T. S., Vonk, L. A., Buskermolen, J. K., Visser, J., Karperien, M., Bleys, R. L A W, Dhert, W. J A, and Saris, D. B F

Published
2015

41. Combination of bone morphogenetic protein-2 plasmid DNA with chemokine CXCL12 creates an additive effect on bone formation onset and volume

Author

Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, MS Orthopaedie Spine, Wegman, F., Poldervaart, M. T., van der Helm, Y. J., Oner, F. C., Dhert, W. J., Alblas, J., Orthopaedie Onderzoek, Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, MS Orthopaedie Spine, Wegman, F., Poldervaart, M. T., van der Helm, Y. J., Oner, F. C., Dhert, W. J., and Alblas, J.

Published
2015

42. Growth factor-induced osteogenesis in a novel radiolucent bone chamber

Author

MS Orthopaedie Spine, Regenerative Medicine and Stem Cells, Lab Reumatologie/Klinische Immunologie, Orthopaedie Onderzoek, Poldervaart, M. T., van der Stok, J., de Haas, M., ‘T Hart, M. C., Oner, F. Cumhur, Dhert, W. J A, Weinans, H., Alblas, J., MS Orthopaedie Spine, Regenerative Medicine and Stem Cells, Lab Reumatologie/Klinische Immunologie, Orthopaedie Onderzoek, Poldervaart, M. T., van der Stok, J., de Haas, M., ‘T Hart, M. C., Oner, F. Cumhur, Dhert, W. J A, Weinans, H., and Alblas, J.

Published
2015

43. Overexpression of hsa-miR-148a promotes cartilage production and inhibits cartilage degradation by osteoarthritic chondrocytes

Author

Vonk, L A, Kragten, A H M, Dhert, W J A, Saris, D B F, Creemers, L B, Vonk, L A, Kragten, A H M, Dhert, W J A, Saris, D B F, and Creemers, L B

Abstract

OBJECTIVE: Hsa-miR-148a expression is decreased in Osteoarthritis (OA) cartilage, but its functional role in cartilage has never been studied. Therefore, our aim was to investigate the effects of overexpressing hsa-miR-148a on cartilage metabolism of OA chondrocytes.DESIGN: OA chondrocytes were transfected with a miRNA precursor for hsa-miR-148a or a miRNA precursor negative control. After 3, 7, 14 and 21 days, real-time PCR was performed to examine gene expression levels of aggrecan (ACAN), type I, II, and X collagen (COL1A1, COL2A1, COl10A1), matrix metallopeptidase 13 (MMP13), a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) and the serpin peptidase inhibitor, clade H (heat shock protein 47), member 1 (SERPINH1). After 3 weeks, DNA content and proteoglycan and collagen content and release were determined. Type II collagen was analyzed at the protein level by Western blot.RESULTS: Overexpression of hsa-miR-148a had no effect on ACAN, COL1A1 and SERPINH1 gene expression, but increased COL2A1 and decreased COL10A1, MMP13 and ADAMTS5 gene expression. Luciferase reporter assay confirmed direct interaction of miR-148a and COL10A1, MMP13 and ADAMTS5. The matrix deposited by the miR-148a overexpressing cells contained more proteoglycans and collagen, in particular type II collagen. Proteoglycan and collagen release into the culture medium was inhibited, but total collagen production was increased.CONCLUSION: Overexpression of hsa-miR-148a inhibits hypertrophic differentiation and increases the production and deposition of type II collagen by OA chondrocytes, which is accompanied by an increased retention of proteoglycans. Hsa-miR-148a might be a potential disease-modifying compound in OA, as it promotes hyaline cartilage production.

Published
2014

44. Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

Author

Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, CSCA TR1, Tissue Repair, Vonk, L A, de Windt, T S, Kragten, A H M, Beekhuizen, M, Mastbergen, S C, Dhert, W J A, Lafeber, F P J G, Creemers, L B, Saris, D B F, Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, CSCA TR1, Tissue Repair, Vonk, L A, de Windt, T S, Kragten, A H M, Beekhuizen, M, Mastbergen, S C, Dhert, W J A, Lafeber, F P J G, Creemers, L B, and Saris, D B F

Published
2014

46. Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) can be effectively applied for longitudinal cohort evaluation of articular cartilage regeneration

Author

Bekkers, J E J, Bartels, L W, Benink, R J, Tsuchida, A I, Vincken, K L, Dhert, W J A, Creemers, L B, Saris, D B F, Bekkers, J E J, Bartels, L W, Benink, R J, Tsuchida, A I, Vincken, K L, Dhert, W J A, Creemers, L B, and Saris, D B F

Abstract

OBJECTIVE: Delayed gadolinium enhanced magnetic resonance imaging (MRI) of cartilage (dGEMRIC) facilitates non-invasive evaluation of the glycosaminoglycan content in articular cartilage. The primary aim of this study was to show that the dGEMRIC technique is able to monitor cartilage repair following regenerative cartilage treatment.DESIGN: Thirty-one patients with a focal cartilage lesion underwent a dGEMRIC scan prior to cartilage repair surgery and at 3 and 12 months follow-up. At similar time points clinical improvement was monitored using the Knee injury and Osteoarthritis Outcome Score (KOOS) and Lysholm questionnaires. Per MRI scan several regions-of-interest (ROIs) were defined for different locations in the joint. The dGEMRIC index (T1gd) was calculated for each ROI. Repeated-measures analysis of variance (RMANOVA) analysis was used to evaluate improvement in clinical scores and MRI T1gd over time. Also regression analysis was performed to show the influence of local repair on cartilage quality at distant locations in the knee.RESULTS: Clinical scores and the dGEMRIC T1gd per ROI showed a statistically significant improvement (P < 0.01), from baseline, at 12 months follow-up. Also, improvement from baseline in T1gd of the ROI defining the treated cartilage defect showed a direct relationship (P < 0.007) to the improvement of the T1gd of ROI at other locations in the joint.CONCLUSIONS: The dGEMRIC MRI protocol is a useful method to evaluate cartilage repair. In addition, local cartilage repair influenced the cartilage quality at other location in the joint. These findings validate the use of dGEMRIC for non-invasive evaluation of the effects of cartilage regeneration.

Published
2013

47. Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) can be effectively applied for longitudinal cohort evaluation of articular cartilage regeneration

Author

Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, Bekkers, J E J, Bartels, L W, Benink, R J, Tsuchida, A I, Vincken, K L, Dhert, W J A, Creemers, L B, Saris, D B F, Faculteit Diergeneeskunde, LS Equine Muscoskeletal Biology, Onderzoek, Bekkers, J E J, Bartels, L W, Benink, R J, Tsuchida, A I, Vincken, K L, Dhert, W J A, Creemers, L B, and Saris, D B F

Published
2013

48. Comparative study of depth-dependent characteristics of equine and human osteochondral tissue from the medial and lateral femoral condyles

Author

Dep Gezondheidszorg Paard, LS Equine Muscoskeletal Biology, Afd Veterinaire Anesthesiologie, Veterinair Pathologisch Diagnostisch Cnt, Faculteit Diergeneeskunde, Onderzoek, ES TR, Malda, J, Benders, K E M, Klein, T J, de Grauw, J C, Kik, M J L, Hutmacher, D W, Saris, D B F, van Weeren, P R, Dhert, W J A, Dep Gezondheidszorg Paard, LS Equine Muscoskeletal Biology, Afd Veterinaire Anesthesiologie, Veterinair Pathologisch Diagnostisch Cnt, Faculteit Diergeneeskunde, Onderzoek, ES TR, Malda, J, Benders, K E M, Klein, T J, de Grauw, J C, Kik, M J L, Hutmacher, D W, Saris, D B F, van Weeren, P R, and Dhert, W J A

Published
2012

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