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41 results on '"Devendran, Citsabehsan"'

1. Bacteriophages evolve enhanced persistence to a mucosal surface

Author

Chin, Wai Hoe, Kett, Ciaren, Cooper, Oren, Müseler, Deike, Zhang, Yaqi, Bamert, Rebecca S., Patwa, Ruzeen, Woods, Laura C., Devendran, Citsabehsan, Korneev, Denis, Tiralongo, Joe, Lithgow, Trevor, McDonald, Michael J., Neild, Adrian, and Barr, Jeremy J.

Published
2022

17. Bacteriophage adaptation to a mammalian mucosa reveals a trans–domain evolutionary axis

Author

Chin, Wai Hoe, primary, Kett, Ciaren, additional, Cooper, Oren, additional, Müseler, Deike, additional, Zhang, Yaqi, additional, Bamert, Rebecca S, additional, Patwa, Ruzeen, additional, Woods, Laura C, additional, Devendran, Citsabehsan, additional, Tiralongo, Joe, additional, Lithgow, Trevor, additional, McDonald, Michael J, additional, Neild, Adrian, additional, and Barr, Jeremy J, additional

Published
2021
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18. Bacteriophages evolve enhanced persistence to a mucosal surface.

Author

Wai Hoe Chin, Kett, Ciaren, Cooper, Oren, Müseler, Deike, Yaqi Zhang, Bamert, Rebecca S., Patwa, Ruzeen, Woods, Laura C., Devendran, Citsabehsan, Korneev, Denis, Tiralongo, Joe, Lithgow, Trevor, McDonald, Michael J., Neild, Adrian, and Barr, Jeremy J.

Subjects
GENETIC recombination, BACTERIOPHAGES, BACTERIAL communities, LABS on a chip, GLYCANS
Abstract

The majority of viruses within the gut are obligate bacterial viruses known as bacteriophages (phages). Their bacteriotropism underscores the study of phage ecology in the gut, where they modulate and coevolve with gut bacterial communities. Traditionally, these ecological and evolutionary questions were investigated empirically via in vitro experimental evolution and, more recently, in vivo models were adopted to account for physiologically relevant conditions of the gut. Here, we probed beyond conventional phage-bacteria coevolution to investigate potential tripartite evolutionary interactions between phages, their bacterial hosts, and the mammalian gut mucosa. To capture the role of the mammalian gut, we recapitulated a life-like gut mucosal layer using in vitro lab-on-a-chip devices (to wit, the gut-on-a-chip) and showed that the mucosal environment supports stable phage-bacteria coexistence. Next, we experimentally coevolved lytic phage populations within the gut-on-a-chip devices alongside their bacterial hosts. We found that while phages adapt to the mucosal environment via de novo mutations, genetic recombination was the key evolutionary force in driving mutational fitness. A single mutation in the phage capsid protein Hoc--known to facilitate phage adherence to mucus--caused altered phage binding to fucosylated mucin glycans. We demonstrated that the altered glycan-binding phenotype provided the evolved mutant phage a competitive fitness advantage over its ancestral wild-type phage in the gut-on-a-chip mucosal environment. Collectively, our findings revealed that phages--in addition to their evolutionary relationship with bacteria--are able to evolve in response to a mammalian-derived mucosal environment. [ABSTRACT FROM AUTHOR]

Published
2022
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27. Self-Aligned Acoustofluidic Particle Focusing and Patterning in Microfluidic Channels from Channel-Based Acoustic Waveguides

Author

Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Collins, David, Han, Jongyoon, O’Rorke, Richard, Devendran, Citsabehsan, Ma, Zhichao, Neild, Adrian, Ai, Ye, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Collins, David, Han, Jongyoon, O’Rorke, Richard, Devendran, Citsabehsan, Ma, Zhichao, Neild, Adrian, and Ai, Ye

Abstract

Acoustic fields have been widely used for manipulation of particles and cells within microfluidic systems. In this Letter, we explore a novel acoustofluidic phenomenon for particle patterning and focusing, where a periodic acoustic pressure field is produced parallel to internal channel boundaries with the imposition of either a traveling or standing surface acoustic wave (SAW). This effect results from the propagation and intersection of edge waves from the channel walls according to the Huygens-Fresnel principle and classical wave fronts from the substrate-fluid interface. We demonstrate versatile control over this effect to produce both one- and two-dimensional acoustic patterning from one-dimensional SAW fields and its utility for continuous particle focusing. Uniquely, this channel-guided acoustic focusing permits the generation of robust acoustic fields without channel resonance conditions and particle focusing positions that are difficult or impossible to produce otherwise.

Published
2018

28. Microfluidic batch process platforms for lab on a chip applications using acoustofluidics

Author

Devendran, Citsabehsan

Subjects
Uncategorized
Abstract

The inception of microfluidics, utilising borrowed technology from the microelectronics industry, has enabled a wide range of applications in various fields ranging from engineering to biochemistry. Advancements in terms of microfabrication and micro-scale fluid control has led to the rise of Lab on a Chip. These systems aim to replicate the results of conventional laboratory procedures in miniaturised systems. Lab on a Chip systems, offer immense potential for a wide range of diagnostic and therapeutic tools. Furthermore, when sample volumes are extremely scarce, rendering conventional diagnostic methods and continuous flow micro fluidic techniques impractical, other methods need to be established. To this end, microfluidic batch process systems offers a solution, although relatively underdeveloped. Batch process systems are a single or multi-stage process in which a certain quantity of inputs are processed to achieve the desired outcome one sample set at a time. It should be noted, as these systems operate at a much smaller scale, some conventional forcing techniques, such as centrifugation are no longer practical. Therefore, different actuation mechanisms need to be developed to replicate the results of their larger scale conventional laboratory and continuous flow counterparts. Acoustic excitation is a potential actuation mechanism which enables the handling of micron-sized particles and cells. Here, we look at different acoustic excitation methods and the underlying principles that allow acoustofluidic systems to manipulate particles for sample preparation and as point- of-care diagnostic tools. In this thesis, three systems are developed to perform particle manipulation both in liquid and air based batch process systems. Firstly, an open bulk acoustic wave system, allowing the ease of external gripping mechanisms is developed to perform size-deterministic separation of 3 μm and 10 μm particles. The task of particle separation is further explored using a different underlying principle and actuation method, and separation of 3.1 μm and 5.1 μm is achieved utilising surface acoustic waves, a different excitation mechanism that enables operation at relatively higher frequencies. Finally, optimisation of an acoustic resonator in air is carried out and serves as a building block for a complete 3-dimensional (3D) acoustic trapping microgripper to be used for individualised particle transport and inspection. Throughout this thesis, a case is made for acoustic based methods to be utilised in developing essential batch process systems for sample preparation and diagnostics.

Published
2017
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