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A biological experiment was conducted to evaluate the ability of glucomannan to adsorb aflatoxin B1(AFB1 and T-2 toxin in gut conditions of broiler chickens. Glucomannan (GM) was tested at 0.1 percent (1kg / ton) on a total of two hundred uniformly weighing five-week-old commercial broiler birds, which were randomly assigned to one of the ten dietary treatments with four replicates each. Four birds were sacrificed at 30 minutes intervals i.e., 0, 30, 60, 90 and 120 minutes from each treatment, and the gut contents were collected. The toxin concentrations in the dried gut samples were estimated and percent of AFB1 and T-2 toxin recovered was measured. The in vivo results revealed that glucomannan had the ability to adsorb Aflatoxin upto 75–90% and T-2 toxin upto 30–35% in gastrointestinal tract of broilers. [ABSTRACT FROM AUTHOR]