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15. Dietary (n-3) polyunsaturated fatty acids affect the kinetics of pro- and antiinflammatory responses in mice with Pseudomonas aeruginosa lung infection

Author

Tiesset, Helene, Pierre, Maud, Desseyn, Jean-Luc, Guery, Benoit, Beermann, Christopher, Galabert, Claude, Gottrand, Frederic, and Husson, Marie-Odile

Subjects
Pseudomonas aeruginosa infections -- Physiological aspects, Pseudomonas aeruginosa infections -- Diet therapy, Unsaturated fatty acids -- Health aspects, Inflammation -- Evaluation, Food/cooking/nutrition
Abstract

The underlying mechanisms by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) affect host resistance to Pseudomonas aeruginosa are unclear. The aim of this study was to determine their role on the kinetic of pro- and antiinflammatory response in lung infection. Mice fed either a control diet or a diet enriched with EPA and DHA were infected intratracheally and we studied lung expression of proinflammatory markers [CXCL1, interleukin (IL)-6, tumor necrosis factor[alpha], antiinflammatory markers (IL-10, A20, and I[kappa]B[alpha]), and PPAR[alpha] and PPAR[gamma]. The inflammatory response was assessed using recruitment of neutrophils and macrophages into bronchoalveolar lavage fluid, bacterial clearance from the lung, pulmonary injury, and 7-d survival rate. Compared with the control group, EPA and DHA delayed the expression of proinflammatory markers during the first 2 h (P < 0.05), upregulated proinflammatory marker expression (P < 0.05), and induced overexpression of antiinflammatory markers at 8 h (P < 0.05), enhanced recruitment of neutrophils at 16 h (P < 0.05), and induced PPAR[alpha] and PPAR[gamma] overexpression at 4 and 8 h (P < 0.01), respectively. Pulmonary bacterial load decreased and pulmonary injury and mortality were reduced during the first 24 h (P< 0.05). In conclusion, EPA and DHA modulate the balance between pro- and antiinfiammatory cytokines, alter the early response of the host to P. aeruginosa infection, and affect the early outcome of infection.

Published
2009

16. Impact and consequences of intensive chemotherapy on intestinal barrier and microbiota in acute myeloid leukemia: the role of mucosal strengthening

Author

Hueso, Thomas, Ekpe, Kenneth, Mayeur, Camille, Gatse, Anna, Joncquel-Chevallier Curt, Marie, Gricourt, Guillaume, Rodriguez, Christophe, Burdet, Charles, Ulmann, Guillaume, Neut, Christel, Amini, Salah-Eddine, Lepage, Patricia, Raynard, Bruno, Willekens, Christophe, Micol, Jean-Baptiste, de Botton, Stéphane, Yakoub-Agha, Ibrahim, Gottrand, Frédéric, Desseyn, Jean-Luc, Thomas, Muriel, Woerther, Paul-Louis, Seguy, David, Institute for Translational Research in Inflammation - U 1286 (INFINITE (Ex-Liric)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Universitaire de Lille (CHU Lille), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Emergence de la résistance bactérienne in vivo (EA3964), Université Paris Diderot - Paris 7 (UPD7), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Département de cancérologie de l'enfant et de l'adolescent [Gustave Roussy], Institut Gustave Roussy (IGR), Département d’Innovation Thérapeutique et essais précoces [Gustave Roussy] (DITEP), Centre hospitalier universitaire Henri-Mondor [Créteil], Dynamic Microbiology - EA 7380 (DYNAMIC), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service des Maladies de l'Appareil Digestif et de la Nutrition [CHRU Lille], Hôpital Claude Huriez [Lille], CHU Lille-CHU Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), University Hospital of Lille, MSD, École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU Lille, and Tâche, Roselyne

Subjects
Adult, Male, [SDV]Life Sciences [q-bio], Mice, Transgenic, chemotherapy, Feces, Mice, mucus, Antineoplastic Combined Chemotherapy Protocols, microbiota, Animals, Humans, acute leukemia, Intestinal Mucosa, lcsh:RC799-869, Aged, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Induction Chemotherapy, Middle Aged, Fatty Acids, Volatile, Gastrointestinal Microbiome, [SDV] Life Sciences [q-bio], Leukemia, Myeloid, Acute, intestinal barrier, Dysbiosis, Female, lcsh:Diseases of the digestive system. Gastroenterology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Research Article, Research Paper
Abstract

International audience; Induction chemotherapy (7 + 3 regimen) remains the gold standard for patients with acute myeloid leukemia (AML) but is responsible for gut damage leading to several complications such as bloodstream infection (BSI). We aimed to investigate the impact of induction chemotherapy on the intestinal barrier of patients with AML and in wild-type mice. Next, we assessed the potential benefit of strengthening the mucosal barrier in transgenic mice releasing a recombinant protein able to reinforce the mucus layer (Tg222). In patients, we observed a decrease of plasma citrulline, which is a marker of the functional enterocyte mass, of short-chain fatty acids and of fecal bacterial load, except forEscherichia coliandEnterococcusspp., which became dominant. Both the alpha and beta-diversities of fecal microbiota decreased. In wild-type mice, citrulline levels decreased under chemotherapy along with an increase ofE. coliandEnterococcusspp load associated with concomitant histologic impairment. By comparison with wild-type mice, Tg222 mice, 3 days after completing chemotherapy, had higher citrulline levels, a faster healing epithelium, and preserved alpha-diversity of their intestinal microbiota. This was associated with reduced bacterial translocations. Our results highlight the intestinal damage and the dysbiosis induced by the 7 + 3 regimen. As a proof of concept, our transgenic model suggests that strengthening the intestinal barrier is a promising approach to limit BSI and improve AML patients' outcome.

Published
2020
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17. Omega-3 polyunsaturated fatty acids improve host response in chronic Pseudomonas aeruginosa lung infection in mice

Author

Pierre, Maud, Husson, Marie-Odile, Le Berre, Rozenn, Desseyn, Jean-Luc, Galabert, Claude, Beghin, Laurent, Beermann, Christopher, Dagenais, Andre, Berthiaume, Yves, Cardinaud, Bruno, Barbry, Pascal, Gottrand, Frederic, and Guery, Benoit P.

Subjects
Omega-3 fatty acids -- Research, Omega-3 fatty acids -- Health aspects, Pseudomonas aeruginosa infections -- Research, Pseudomonas aeruginosa infections -- Risk factors, Pulmonary alveoli -- Research, Biological sciences
Abstract

Pseudomonas aeruginosa is a gram-negative bacilli frequently encountered in human pathology. This pathogen is involved in a large number of nosocomial infections and chronic diseases. Herein we investigated the effects of polyunsaturated fatty acids (PUFA) in chronic Pseudomonas aeruginosa lung infection. C57BL/6 mice were fed for 5 wk with specifically designed diets with high contents in either omega-3 ([omega]-3) or [omega]-6 PUFA and compared to a control diet. P. aeruginosa included in agarose beads was then instilled intratracheally, and the animals were studied for 7 days. On the 4th day, the mice fed with the [omega]-3 diet had a higher lean body mass gain and a lower [omega]-6:[omega]-3 ratio of fatty acids extracted from the lung tissue compared with the other groups (P < 0.05). The [omega]-3 group had the lowest mortality. Distal alveolar fluid clearance (DAFC) as well as the inflammatory response and the cellular recruitment were higher in the [omega]-3 group on the 4th day. The effect on DAFC was independent of [alpha]-epithelial [Na.sup.+] channels ([alpha]-ENaC), [beta]-ENaC, and [[alpha].sub.1]-Na-K-ATPase mRNA expressions, which were not altered by the different diets. In conclusion, a diet enriched in [omega]-3 PUFA can change lung membrane composition and improve survival in chronic pneumonia. This effect on survival is probably multifactorial involving the increased DAFC capacity as well as the optimization of the initial inflammatory response. This work suggests that a better control of the [omega]-6/[omega]-3 PUFA balance may represent an interesting target in the prevention and/or control of P. aeruginosa infection in patients. alveolar liquid clearance doi:10.1152/ajplung.00337.2006.

Published
2007

23. [Gel-forming mucins structure governs mucus gels viscoelasticity]

Author

Demouveaux, Bastien, Gouyer, Valérie, Magnien, Mylene, Plet, Ségolène, Gottrand, Frederic, Narita, Tetsuharu, Desseyn, Jean-Luc, Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Inserm, Université de Lille, CHU Lille, Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286, Lille Inflammation Research International Center - U 995 [LIRIC], Lille Inflammation Research International Center (LIRIC) - U995, and Université de Lille, LillOA

Subjects
[SDV] Life Sciences [q-bio], Mucus, Viscosity, [SDV]Life Sciences [q-bio], Mucins, Animals, Humans, Rheology, Gels, Elasticity
Abstract

International audience; Mucus is the first line of innate mucosal defense in all mammals. Gelforming mucins control the rheological properties of mucus hydrogels by forming a network in which hydrophilic and hydrophobic regions coexist, and it has been revealed that the network is formed through both covalent links and reversible links such as hydrophobic interactions in order to modulate the structure as a function of the physiological necessities. Here, we review the structure and functions of the mucus in terms of the gel-forming mucins protein-protein interactions, also called interactome. Since it is difficult to characterize the low energy reversible interactions due to their dependence on physico-chemical environment, their role is not well understood. Still, they constitute a promising target to counteract mucus abnormalities observed in mucus-associated diseases.

Published
2018
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24. Long-term dietary (n-3) polyunsaturated fatty acids show benefits to the lungs of Cftr F508del mice

Author

Portal, Céline, Gouyer, Valérie, Léonard, Renaud, Husson, Marie-Odile, Gottrand, Frédéric, Desseyn, Jean-Luc, Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Inserm, Université de Lille, CHU Lille, CNRS, Lille Inflammation Research International Center - U 995 [LIRIC], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Lille Inflammation Research International Center (LIRIC), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Time Factors, Physiology, [SDV]Life Sciences [q-bio], lcsh:Medicine, Pathology and Laboratory Medicine, Mice, Immune Physiology, Medicine and Health Sciences, lcsh:Science, Immune Response, Lung, Innate Immune System, Ecology, Pseudomonas Aeruginosa, Animal Models, respiratory system, Trophic Interactions, Body Fluids, Bacterial Pathogens, Experimental Organism Systems, Community Ecology, Medical Microbiology, Cytokines, Female, lipids (amino acids, peptides, and proteins), Growth and Development, Anatomy, Pathogens, Research Article, Histology, Immunology, Mouse Models, Research and Analysis Methods, Microbiology, Model Organisms, Signs and Symptoms, Diagnostic Medicine, Pseudomonas, Fatty Acids, Omega-3, Animals, Microbial Pathogens, Nutrition, Inflammation, Bacteria, Ecology and Environmental Sciences, lcsh:R, Organisms, Biology and Life Sciences, Biological Transport, Molecular Development, Diet, respiratory tract diseases, Mucus, Immune System, Dietary Supplements, lcsh:Q, Developmental Biology
Abstract

INTRODUCTION: The pro-inflammatory status of cystic fibrosis (CF) patients promotes pulmonary colonization with opportunist and pathogenic bacteria, which is favored by a sticky mucus. Oral supplementation with (n-3) long chain polyunsaturated fatty acids (LC-PUFA) has shown anti-inflammatory effects. The aim of this study was to demonstrate the positive effects of a long-term diet enriched in (n-3) LC-PUFA on the lungs of Cftr F508del mice. MATERIALS AND METHODS: Breeding CftrDeltaF508del/+ mice received a control diet or a diet enriched in (n-3) LC-PUFA for 5 weeks before mating, gestation and lactation. After weaning, the offspring were given the same diet as their mother until post-natal day 60. The effects of (n-3) LC-PUFA supplementation on the lungs were evaluated in homozygous Cftr F508del mice and their wild-type littermates after acute lung inflammation induced by Pseudomonas aeruginosa lipopolysaccharide (LPS) inhalation. RESULTS: (n-3) LC-PUFA enrichment of mothers contributes to enrichment of mammary milk and cell membrane of suckling pups. Cftr F508del mice exhibited growth retardation and lung damage with collapsed alveoli, hyperplasia of bronchial epithelial cells and inflammatory cell infiltration. The (n-3) LC-PUFA diet corrected the growth delay of Cftr F508del mice and decreased hyperplasia of bronchial epithelial cells. Besides decreasing metaplasia of Club cells after LPS inhalation, (n-3) LC-PUFA modulated lung inflammation and restricted lung damage. CONCLUSION: Long-term (n-3) LC-PUFA supplementation shows moderate benefits to the lungs of Cftr F508del mice. 13

Published
2018
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25. Muc5b-deficient mice develop early histological lung abnormalities

Author

Valque, Hélène, primary, Gouyer, Valérie, additional, Duez, Catherine, additional, Leboeuf, Christophe, additional, Marquillies, Philippe, additional, Le Bert, Marc, additional, Plet, Ségolène, additional, Ryffel, Bernhard, additional, Janin, Anne, additional, Gottrand, Frédéric, additional, and Desseyn, Jean-Luc, additional

Published
2019
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27. Dietary n-3 fatty acids have suppressive effects on mucin upregulation in mice infected with Pseudomonas aeruginosa

Author

Gottrand Frédéric, Galabert Claude, Béghin Laurent, Husson Marie-Odile, Demeyer Dominique, Pierre Maud, Tetaert Daniel, Beermann Christopher, Guery Benoit, and Desseyn Jean-Luc

Subjects
Diseases of the respiratory system, RC705-779
Abstract

Abstract Background Mucin hypersecretion and mucus plugging in the airways are characteristic features of chronic respiratory diseases like cystic fibrosis (CF) and contribute to morbidity and mortality. In CF, Pseudomonas aeruginosa superinfections in the lung exacerbate inflammation and alter mucus properties. There is increasing evidence that n-3 polyunsaturated fatty acids (PUFAs) exhibit anti-inflammatory properties in many inflammatory diseases while n-6 PUFA arachidonic acid (AA) favors inflammatory mediators such as eicosanoids prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) that may enhance inflammatory reactions. This suggests that n-3 PUFAs may have a protective effect against mucus over-production in airway diseases. Therefore, we hypothesized that n-3 PUFAs may downregulate mucins expression. Methods We designed an absolute real-time PCR assay to assess the effect of a 5-week diet enriched either with n-3 or n-6 PUFAs on the expression of large mucins in the lungs of mice infected by P. aeruginosa. Results Dietary fatty acids did not influence mucin gene expression in healthy mice. Lung infection induced an increase of the secreted gel-forming mucin Muc5b and a decrease of the membrane bound mucin Muc4. These deregulations are modulated by dietary fatty acids with a suppressive effect of n-3 PUFAs on mucin (increase of Muc5b from 19-fold up to 3.6 × 105-fold for the n-3 PUFAs treated group and the control groups, respectively, 4 days post-infection and decrease of Muc4 from 15-fold up to 3.2 × 104-fold for the control and the n-3 PUFAs treated groups, respectively, 4 days post-infection). Conclusion Our data suggest that n-3 PUFAs enriched diet represents an inexpensive strategy to prevent or treat mucin overproduction in pulmonary bacterial colonization.

Published
2007
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28. Impact and consequences of intensive chemotherapy on intestinal barrier and microbiota in acute myeloid leukemia: the role of mucosal strengthening.

Author

Hueso, Thomas, Ekpe, Kenneth, Mayeur, Camille, Gatse, Anna, Joncquel-Chevallier Curt, Marie, Gricourt, Guillaume, Rodriguez, Christophe, Burdet, Charles, Ulmann, Guillaume, Neut, Christel, Amini, Salah-Eddine, Lepage, Patricia, Raynard, Bruno, Willekens, Christophe, Micol, Jean-Baptiste, De Botton, Stéphane, Yakoub-Agha, Ibrahim, Gottrand, Frédéric, Desseyn, Jean-Luc, and Thomas, Muriel

Published
2021
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29. Assembly of the Respiratory Mucin MUC5B: A new model for a gel-forming mucin

Author

Ridley, Caroline, Kouvatsos, Nikos, Raynal, Bertrand D, Howard, Marj, Collins, Richard F, Desseyn, Jean-Luc, Jowitt, Thomas A, Baldock, Clair, Davis, C William, Hardingham, Timothy E, Thornton, David J, University of Manchester [Manchester], Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Department of Medicine and Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC)-University of North Carolina System (UNC), and This work was supported by grants from Cystic Fibrosis Foundation Therapeutics (THORNT07XXX0) and Biotechnology and Biological Sciences Research Council (BBSRC). The Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, is supported by core funding from the Wellcome Trust (088785/Z/09/Z).

Subjects
Recombinant Protein Expression, Microscopy, Single Particle Analysis, Cystic Fibrosis, Respiratory System, Glycobiology and Extracellular Matrices, Hydrogen-Ion Concentration, Electron, Mucin-5B, Goblet Cell, Mucus, Microscopy, Electron, Transmission, Mucin, Transmission, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Calcium, Analytical Ultracentrifugation, Ultracentrifugation
Abstract

Background: Mucin polymer formation is a complex intracellular process. Results: MUC5B N-terminal D3-domains form reversible pH-sensitive calcium mediated cross-links between linear MUC5B polymer chains. Conclusion: Intracellular assembly of MUC5B generates disulfide-bonded polymers which form calcium mediated condensed networks in secretory granules. Significance: This identifies a new model for mucin assembly that may be common to other polymeric mucins., Mucins are essential components in mucus gels that form protective barriers at all epithelial surfaces, but much remains unknown about their assembly, intragranular organization, and post-secretion unfurling to form mucus. MUC5B is a major polymeric mucin expressed by respiratory epithelia, and we investigated the molecular mechanisms involved during its assembly. Studies of intact polymeric MUC5B revealed a single high affinity calcium-binding site, distinct from multiple low affinity sites on each MUC5B monomer. Self-diffusion studies with intact MUC5B showed that calcium binding at the protein site catalyzed reversible cross-links between MUC5B chains to form networks. The site of cross-linking was identified in the MUC5B D3-domain as it was specifically blocked by D3 peptide antibodies. Biophysical analysis and single particle EM of recombinant MUC5B N terminus (D1D2D′D3; NT5B) and subdomains (D1, D1-D2, D2-D′-D3, and D3) generated structural models of monomers and disulfide-linked dimers and suggested that MUC5B multimerizes by disulfide linkage between D3-domains to form linear polymer chains. Moreover, these analyses revealed reversible homotypic interactions of NT5B at low pH and in high calcium, between disulfide-linked NT5B dimers, but not monomers. These results enable a model of MUC5B to be derived, which predicts mechanisms of mucin intracellular assembly and storage, which may be common to the other major gel-forming polymeric mucins.

Published
2014
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33. Systematic gene overexpression inCandida albicansidentifies a regulator of early adaptation to the mammalian gut

Author

Znaidi, Sadri, primary, van Wijlick, Lasse, additional, Hernández-Cervantes, Arturo, additional, Sertour, Natacha, additional, Desseyn, Jean-Luc, additional, Vincent, Frédéric, additional, Atanassova, Ralitsa, additional, Gouyer, Valérie, additional, Munro, Carol A., additional, Bachellier-Bassi, Sophie, additional, Dalle, Frédéric, additional, Jouault, Thierry, additional, Bougnoux, Marie-Elisabeth, additional, and d'Enfert, Christophe, additional

Published
2018
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37. Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier

Author

Gouyer, Valérie, Dubuquoy, Laurent, Robbe-Masselot, Catherine, Neut, Christel, Singer, Elisabeth, Plet, Ségolène, Geboes, Karel, Desreumaux, Pierre, Gottrand, Frederic, Desseyn, Jean Luc, Lille Inflammation Research International Center - U 995 (LIRIC), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Inserm, Université de Lille, CHU Lille, Lille Inflammation Research International Center - U 995 [LIRIC], Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), and Université de Lille, LillOA

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], Mesh:Animals, Mesh:Citrobacter rodentium/immunology, Mesh:Cysteine/chemistry, Mesh:Escherichia coli/immunology, Mesh:Gastrointestinal Microbiome/immunology, Mesh:Inflammatory Bowel Diseases/immunology, Mesh:Intestinal Mucosa/microbiology, Mesh:Intestines/microbiology, Mesh:Mice, Mesh:Inbred C57BL, Mesh:Mucin-2/genetics, Mesh:Mucin-2/metabolism, Mesh:Mucus/metabolism, Mesh:Epithelial Cells, Mesh:Mucin-6/metabolism, Mesh:Transgenic, Mesh:Goblet Cells, Mesh:Intestinal Mucosa/metabolism, Mesh:Protein Structure, Mesh:Tertiary, Mesh:Inflammatory Bowel Diseases/metabolism, Mesh:Tight Junctions/physiology, Mesh:Intestinal Mucosa/cytology, Mesh:Lactobacillus/isolation & purification, Mesh:Mucin-3/metabolism, Mesh:Microspheres, Mesh:Glycosylation, Mesh:Mucin-1/metabolism, MESH: Mesh:Glycosylation, MESH: Mesh:Microspheres, MESH: Mesh:Mucin-3/metabolism, MESH: Mesh:Lactobacillus/isolation & purification, ComputingMilieux_MISCELLANEOUS, MESH: Mesh:Mucin-1/metabolism
Abstract

International audience

Published
2015
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42. A highly parallel conditional overexpression screen, in vivo, identifies a new regulator of Candida albicans commensalism

Author

Znaidi, Sadri, primary, Cervantes, Arturo Hernández, additional, Desseyn, Jean-Luc, additional, Sertour, Natacha, additional, Vincent, Frédéric, additional, Gouyer, Valérie, additional, Dalle, Frédéric, additional, Jouault, Thierry, additional, Bougnoux, Marie-Élisabeth, additional, and d’Enfert, Christophe, additional

Published
2015
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44. A novel lipoxygenase in olives (Olea europaea L.), with dual positional specificity, expressed late during fruit development

Author

Palmieri-Thiers, Cynthia, Canaan, Stéphane, Brunini, Virginie, Lorenzi, Vannina, Tomi, Félix, Desseyn, Jean-Luc, Berti, Liliane, Maury, Jacques, Sciences pour l'environnement (SPE), Centre National de la Recherche Scientifique (CNRS)-Université Pascal Paoli (UPP), Laboratoire Enzymologie Interfaciale et de Physiologie de la Lipolyse - UPR9025 (EIPL), Université de la Méditerranée - Aix-Marseille 2-Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Université Pascal Paoli (UPP)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), and Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille

Subjects
integumentary system, food and beverages, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Abstract

International audience; Plant lipoxygenases (LOXs) are a class of widespread dioxygenase catalyzing the hydroperoxidation of polyunsaturated fatty acids. Although multiple isoforms of LOX have been detected in a wide range of plants, their physiological role is still unclear. With the aim to clarify the occurrence of LOXs and their contribution to the elaboration of the olive oil aroma, we carried out the biochemical and molecular characterization of the LOX isoform expressed during olive development. A full-length cDNA was isolated by Reverse Transcription PCR and Rapid amplification of cDNA end carried out on total RNA from mature olives. The 2852 bp sequence displays an open reading frame of 2592 bp encoding a putative polypeptide of 864 amino acids with a calculated molecular mass of 98.4 kDa and a pI of 5.95. The olive LOX is a type-1 LOX and shows a high degree of identity towards hazelnut (77.3%), tobacco (76.3%) and almond (75.5%) LOXs. The recombinant enzyme produced in E. coli shows a dual positional specificity, as it releases both 9- and 13-hydroperoxide of linoleic acid in a 2:1 ratio. Although LOX activity was detected throughout the olive development, the 9/13-LOX is mainly expressed at late developmental stages. Our data suggest the presence of at least two LOX isoforms in black olives, and that the 9/13-LOX is associated with the ripening and senescence processes. However, due to its dual positional specificity ant its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered.

Published
2008

45. Architecture of the gel-forming mucins

Author

Desseyn, Jean-Luc, Gouyer, Valérie, Tetaert, Daniel, Lille Inflammation Research International Center - U 995 (LIRIC), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Université de Lille, LillOA, Université de Lille, Inserm, CHU Lille, Lille Inflammation Research International Center - U 995 [LIRIC], Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 [JPArc], Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 (JPArc), and Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio]
Abstract

International audience; This review article focuses on the multi-domainorganization of the five human gel-forming mucinsMUC2, MUC5B, MUC5AC, MUC6 and MUC19. Gel-forming mucins are O-glycoproteins with highmolecular mass that are secreted at the cell surface ofmany secretory organs by specialized epithelial cells.They form dimers through their carboxy-terminiregions and then multimers through their amino-termini regions before being secreted into the lumenof organs as long linear filaments. In addition, anoverview of their genomic organization is given andwe took the opportunity of this article to determine the genomic organisation of the 5'-end region of MUC5AC. Finally, we discuss thepolymorphism of mucins and a few features of long messengers.

Published
2008

46. Dietary Pectin–Derived Acidic Oligosaccharides Improve the Pulmonary Bacterial Clearance ofPseudomonas aeruginosaLung Infection in Mice by Modulating Intestinal Microbiota and Immunity

Author

Bernard, Henry, primary, Desseyn, Jean-Luc, additional, Bartke, Nana, additional, Kleinjans, Lennart, additional, Stahl, Bernd, additional, Belzer, Clara, additional, Knol, Jan, additional, Gottrand, Frédéric, additional, and Husson, Marie-Odile, additional

Published
2014
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47. Characterization of mouse muc6 and evidence of conservation of the gel-forming mucin gene cluster between human and mouse

Author

Desseyn, Jean-Luc, Laine, Anne, Mucines Epitheliales : du Gene a la Fonction, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, This work was supported by l’Association de Recherche contre le Cancer (No. 4458) and l’Institut National de la Santé et de la Recherche Médicale, and The authors thank Dominique Demeyer for the nucleotide sequencing and Marie-Paule Delescaut for RNA extractions.

Subjects
Base Sequence, Blotting, Reverse Transcriptase Polymerase Chain Reaction, [SDV]Life Sciences [q-bio], Gene Expression Profiling, Molecular Sequence Data, Mucins, Chromosome Mapping, Blotting, Southern, Mice, Animals, Humans, Southern, Mucin-6, DNA Primers
Abstract

International audience; Using degenerate primers designed from conserved cysteine-rich domains of gel-forming mucins, we cloned two new mouse mucin cDNAs. Blast searching showed that they belong to the same new gene assigned to chromosome 7 band F5. This gene is clustered with the three secreted large gel-forming mucins Muc2, Muc5ac, and Muc5b in a region that exhibits synteny with human chromosome 11p15. Computer analysis and sequence alignments with mucin genes predict that the new gene is composed of 33 exons and spans 30 kb from the initiation ATG codon to the Stop codon. Sequence similarities, domain organization of the deduced peptide, and expression analysis allow us to conclude that this newly cloned mouse gene is Muc6, i.e., the mouse ortholog of human MUC6. Like those of their human homologs, the genomic order and arrangement of the four mucins within the cluster of mucin genes are conserved.

Published
2003
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48. Organisation du gène de mucine humaine MUC5B et bases moléculaires d’une nouvelle classification des gènes de mucines

Author

Desseyn, Jean-Luc, Laine, Anne, Université de Lille, LillOA, Lille Inflammation Research International Center - U 995 (LIRIC), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], Mucins, Mucines, TGF-beta, von Willebrand factor, MUC5B, Facteur de von Willebrand, ComputingMilieux_MISCELLANEOUS, EGF
Abstract

International audience

Published
1999

49. A lipoxygenase with dual positional specificity is expressed in olives (Olea europaea L.) during ripening

Author

Palmieri-Thiers, Cynthia, Canaan, Stéphane, Brunini, Virginie, Lorenzi, Vannina, Tomi, Félix, Desseyn, Jean-Luc, Garscha, Ulrike, Oliw, Ernst H, Berti, Liliane, Maury, Jacques, Palmieri-Thiers, Cynthia, Canaan, Stéphane, Brunini, Virginie, Lorenzi, Vannina, Tomi, Félix, Desseyn, Jean-Luc, Garscha, Ulrike, Oliw, Ernst H, Berti, Liliane, and Maury, Jacques

Abstract

Plant lipoxygenases (LOXs) are a class of widespread dioxygenases catalysing the hydroperoxidation of polyunsaturated fatty acids. Although multiple isoforms of LOX have been detected in a wide range of plants, their physiological roles remain to be clarified. With the aim to clarify the occurrence of LOXs in olives and their contribution to the elaboration of the olive oil aroma, we cloned and characterized the first cDNA of the LOX isoform which is expressed during olive development. The open reading frame encodes a polypeptide of 864 amino acids. This olive LOX is a type-1 LOX which shows a high degree of identity at the peptide level towards hazelnut (77.3%), tobacco (76.3%) and almond (75.5%) LOXs. The recombinant enzyme shows a dual positional specificity, as it forms both 9- and 13-hydroperoxide of linoleic acid in a 2:1 ratio, and would be defined as 9/13-LOX. Although a LOX activity was detected throughout the olive development, the 9/13-LOX is mainly expressed at late developmental stages. Our data suggest that there are at least two Lox genes expressed in black olives, and that the 9/13-LOX is associated with the ripening and senescence processes. However, due to its dual positional specificity and its expression pattern, its contribution to the elaboration of the olive oil aroma might be considered.

Published
2009
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