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31 results on '"Desprat, Romain"'

2. Generation of patient-specific induced pluripotent stem cell lines with Type 2 Long QT Syndrome and the KCNH2 c.379C > T pathogenic variant

Author

Goual, Lamia, primary, Bounasri, Elisa, additional, Vincenti, Marie, additional, Amédro, Pascal, additional, Desprat, Romain, additional, Bernex, Florence, additional, Lemaitre, Jean-Marc, additional, Pasquié, Jean-Luc, additional, Lacampagne, Alain, additional, Thireau, Jérôme, additional, and Meli, Albano C., additional

Published
2023
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3. Generation of human induced pluripotent stem cell lines (iPSC) from adipose-derived mesenchymal stromal cells from two patients with systemic sclerosis

Author

Velier, Mélanie, Appay, Romain, Arcani, Robin, Desprat, Romain, Simoncini, Stéphanie, Zavarro, Anouck, Noel, Danièle, Bendaass, Lenha, Jorgensen, Christian, Gomes De Pinho, Quentin, Benyamine, Audrey, Granel, Brigitte, Daumas, Aurélie, Dignat George, Francoise, Sabatier, Florence, and Magalon, Jérémy

Published
2025
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4. The Molecular Machinery of Somatic Cell Reprogramming

Author

Desprat, Romain, Milhavet, Ollivier, Lemaitre, Jean-Marc, Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), River Publishers 2014, and Philips, Alexandre

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2022
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5. A single short reprogramming early in life initiates and propagates an epigenetically related mechanism improving fitness and promoting an increased healthy lifespan

Author

Alle, Quentin, primary, Le Borgne, Enora, additional, Bensadoun, Paul, additional, Lemey, Camille, additional, Béchir, Nelly, additional, Gabanou, Mélissa, additional, Estermann, Fanny, additional, Bertrand‐Gaday, Christelle, additional, Pessemesse, Laurence, additional, Toupet, Karine, additional, Desprat, Romain, additional, Vialaret, Jérôme, additional, Hirtz, Christophe, additional, Noël, Danièle, additional, Jorgensen, Christian, additional, Casas, François, additional, Milhavet, Ollivier, additional, and Lemaitre, Jean‐Marc, additional

Published
2022
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8. Generation of three Duchenne Muscular Dystrophy patient-specific induced pluripotent 4 stem cell lines DMD_YoTaz_PhyMedEXp, DMD_RaPer_PhyMedEXp, 5 DMD_OuMen_PhyMedEXp (INSRMi008-A, INSRMi009-A and INSRMi010-A)

Author

Souidi, Monia, Amedro, Pascal, Meyer, Pierre, Desprat, Romain, Lemaitre, Jean-Marc, Rivier, Francois, Lacampagne, Alain, Meli, Albano C, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), and MORNET, Dominique

Subjects
[SDV] Life Sciences [q-bio], musculoskeletal diseases, [SDV]Life Sciences [q-bio]
Abstract

International audience; Abstract: Duchenne Muscular Dystrophy (DMD) is a X-linked degenerative pathology with a prevalence of 1/3600-6000 boys due to the absence of functional dystrophin in muscles. This muscular disease leads to skeletal muscle damages, respiratory failure and in the later stages dilated cardiomyopathy (DCM) leading to heart failure. We generated iPSC lines from three different DMD patients carrying respectively deletions of exons 1, 52 and 55 in the dystrophin gene. The reprogrammed iPSC lines showed expression of pluripotent markers, capacity to differentiate in trilineage embryonic layers and a normal karyotype.

Published
2020
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15. Cellular senescence induces replication stress without affecting DNA replication timing

Author

Rivera-Mulia, Juan Carlos, Schwerer, Helene, Besnard, Emilie, Desprat, Romain, Trevilla-Garcia, Claudia, Sima, Jiao, Bensadoun, Paul, Zouaoui, Anissa, David, Gilbert, Lemaitre, Jean-Marc, Florida State University [Tallahassee] (FSU), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), CHU Montpellier, and Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018
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16. Pathologies des hélicases et vieillissement précoce : modèle d’étude par dérivation de cellules souches pluripotentes induites

Author

Gatinois, Vincent, primary, Desprat, Romain, additional, Corsini, Carole, additional, Puechberty, Jacques, additional, Gaillard, Jean-Baptiste, additional, Schneider, Anouck, additional, Guignard, Thomas, additional, Lavabre-Bertrand, Thierry, additional, Lemaitre, Jean-Marc, additional, and Pellestor, Franck, additional

Published
2019
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19. Predictable dynamic program of timing of DNA replication in human cells

Author

Desprat, Romain, Thierry-Mieg, Danielle, Lailler, Nathalie, Lajugie, Julien, Schildkraut, Carl, Thierry-Mieg, Jean, and Bouhassira, Eric E.

Subjects
DNA replication -- Analysis, Human genome -- Research, Embryonic stem cells -- Research, Nucleotide sequencing -- Usage, Gene expression -- Analysis, Health
Published
2009

20. Embryonic stem cells or induced pluripotent stem cells? A DNA integrity perspective

Author

Bai, Qiang, Desprat, Romain, Klein, Bernard, Lemaitre, Jean-Marc, de Vos, John, Frei, Monique, Cellules souches normales et cancéreuses, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut de recherche en biothérapie (IRB), Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université Montpellier 1 - UFR de Médecine (UM1 Médecine), Université Montpellier 1 (UM1), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Computationnelle (IBC), Institut National de la Recherche Agronomique (INRA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Association Française contre les Myopathies (AFM, Paris, France), and Région Languedoc-Roussillon, France

Subjects
Induced pluripotent stem cells, genome integrity, [SDV.IMM] Life Sciences [q-bio]/Immunology, cell reprogramming, genetic abnormalities, DNA damage, [SDV.IMM]Life Sciences [q-bio]/Immunology, pluripotency
Abstract

International audience; Induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) are two types of pluripotent stem cells that hold great promise for biomedical research and medical applications. iPSCs were initially favorably compared to ESCs. This view was first based on ethical arguments (the generation of iPSCs does not require the destruction of an embryo) and on immunological reasons (it is easier to derive patient HLA-matched iPSCs than ESCs). However, several reports suggest that iPSCs might be characterized by higher occurrence of epigenetic and genetic aberrations than ESCs as a consequence of the reprogramming process. We focus here on the DNA integrity of pluripotent stem cells and examine the three main sources of genomic abnormalities in iPSCs: (1) genomic variety of the parental cells, (2) cell reprogramming, and (3) in vitro cell culture. Recent reports claim that it is possible to generate mouse or human iPSC lines with a mutation level similar to that of the parental cells, suggesting that "genome-friendly" reprogramming techniques can be developed. The issue of iPSC DNA integrity clearly highlights the crucial need of guidelines to define the acceptable level of genomic integrity of pluripotent stem cells for biomedical applications. We discuss here the main issues that such guidelines should address.

Published
2013
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23. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery.

Author

Kiprilov, Enko N, Awan, Aashir, Desprat, Romain, Velho, Michelle, Clement, Christian A, Byskov, Anne Grete, Andersen, Claus Y, Satir, Peter, Bouhassira, Eric E, Christensen, Søren T, Hirsch, Rhoda Elison, Kiprilov, Enko N, Awan, Aashir, Desprat, Romain, Velho, Michelle, Clement, Christian A, Byskov, Anne Grete, Andersen, Claus Y, Satir, Peter, Bouhassira, Eric E, Christensen, Søren T, and Hirsch, Rhoda Elison

Abstract

Human embryonic stem cells (hESCs) are potential therapeutic tools and models of human development. With a growing interest in primary cilia in signal transduction pathways that are crucial for embryological development and tissue differentiation and interest in mechanisms regulating human hESC differentiation, demonstrating the existence of primary cilia and the localization of signaling components in undifferentiated hESCs establishes a mechanistic basis for the regulation of hESC differentiation. Using electron microscopy (EM), immunofluorescence, and confocal microscopies, we show that primary cilia are present in three undifferentiated hESC lines. EM reveals the characteristic 9 + 0 axoneme. The number and length of cilia increase after serum starvation. Important components of the hedgehog (Hh) pathway, including smoothened, patched 1 (Ptc1), and Gli1 and 2, are present in the cilia. Stimulation of the pathway results in the concerted movement of Ptc1 out of, and smoothened into, the primary cilium as well as up-regulation of GLI1 and PTC1. These findings show that hESCs contain primary cilia associated with working Hh machinery. Udgivelsesdato: 2008-Mar-10

Published
2008

25. Genome-scale analysis of metazoan replication origins reveals their organization in specific but flexible sites defined by conserved features

Author

Cayrou, Christelle, primary, Coulombe, Philippe, additional, Vigneron, Alice, additional, Stanojcic, Slavica, additional, Ganier, Olivier, additional, Peiffer, Isabelle, additional, Rivals, Eric, additional, Puy, Aurore, additional, Laurent-Chabalier, Sabine, additional, Desprat, Romain, additional, and Méchali, Marcel, additional

Published
2011
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29. Production of Embryonic and Fetal-Like Red Blood Cells from Human Induced Pluripotent Stem Cells.

Author

Chan-Jung Chang, Mitra, Koyel, Koya, Mariko, Velho, Michelle, Desprat, Romain, Lenz, Jack, and Bouhassira, Eric E.

Subjects
BLOOD cells, INDUCED pluripotent stem cells, ERYTHROPOIESIS, MESENCHYMAL stem cells, FIBROBLASTS
Abstract

We have previously shown that human embryonic stem cells can be differentiated into embryonic and fetal type of red blood cells that sequentially express three types of hemoglobins recapitulating early human erythropoiesis. We report here that we have produced iPS from three somatic cell types: adult skin fibroblasts as well as embryonic and fetal mesenchymal stem cells. We show that regardless of the age of the donor cells, the iPS produced are fully reprogrammed into a pluripotent state that is undistinguishable from that of hESCs by low and high-throughput expression and detailed analysis of globin expression patterns by HPLC. This suggests that reprogramming with the four original Yamanaka pluripotency factors leads to complete erasure of all functionally important epigenetic marks associated with erythroid differentiation regardless of the age or the tissue type of the donor cells, at least as detected in these assays. The ability to produce large number of erythroid cells with embryonic and fetal-like characteristics is likely to have many translational applications. [ABSTRACT FROM AUTHOR]

Published
2011
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30. DNA Methylation Supports Intrinsic Epigenetic Memory in Mammalian Cells.

Author

Yong-Qing Feng, Desprat, Romain, Haiqing Fu, Olivier, Emmanuel, Chii Mei Lin, Lobell, Amanda, Gowda, Shilpa N., Aladjem, Mirit I., and Bouhassira, Eric E.

Subjects
*DNA, *METHYLATION, *GENE silencing, *CHROMATIN, *SOMATIC cells, *DNA replication, *TRANSGENES, *ACETYLATION
Abstract

We have investigated the role of DNA methylation in the initiation and maintenance of silenced chromatin in somatic mammalian cells. We found that a mutated transgene, in which all the CpG dinucleotides have been eliminated, underwent transcriptional silencing to the same extent as the unmodified transgene. These observations demonstrate that DNA methylation is not required for silencing. The silenced CpG-free transgene exhibited all the features of heterochromatin, including silencing of transcriptional activity, delayed DNA replication, lack of histone H3 and H4 acetylation, lack of H3-K4 methylation, and enrichment in tri-methyl-H3-K9. In contrast, when we tested for transgene reactivation using a Cre recombinase-mediated inversion assay, we observed a marked difference between a CpG-free and an unmodified transgene: the CpG-free transgene resumed transcription and did not exhibit markers of heterochromatin whereas the unmodified transgene remained silenced. These data indicate that methylation of CpG residues conferred epigenetic memory in this system. These results also suggest that replication delay, lack of histone H3 and H4 acetylation, H3-K4 methylation, and enrichment in tri-methyl-H3-K9 are not sufficient to confer epigenetic memory. We propose that DNA methylation within transgenes serves as an intrinsic epigenetic memory to permanently silence transgenes and prevent their reactivation. [ABSTRACT FROM AUTHOR]

Published
2006
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31. Embryonic stem cells or induced pluripotent stem cells? A DNA integrity perspective.

Author

Bai Q, Desprat R, Klein B, Lemaître JM, and De Vos J

Subjects
Animals, Cell Culture Techniques, Cell Differentiation, Embryonic Stem Cells immunology, Epigenesis, Genetic, Humans, Induced Pluripotent Stem Cells immunology, Mice, DNA genetics, Embryonic Stem Cells cytology, Genomic Instability, Induced Pluripotent Stem Cells cytology
Abstract

Induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) are two types of pluripotent stem cells that hold great promise for biomedical research and medical applications. iPSCs were initially favorably compared to ESCs. This view was first based on ethical arguments (the generation of iPSCs does not require the destruction of an embryo) and on immunological reasons (it is easier to derive patient HLA-matched iPSCs than ESCs). However, several reports suggest that iPSCs might be characterized by higher occurrence of epigenetic and genetic aberrations than ESCs as a consequence of the reprogramming process. We focus here on the DNA integrity of pluripotent stem cells and examine the three main sources of genomic abnormalities in iPSCs: (1) genomic variety of the parental cells, (2) cell reprogramming, and (3) in vitro cell culture. Recent reports claim that it is possible to generate mouse or human iPSC lines with a mutation level similar to that of the parental cells, suggesting that "genome-friendly" reprogramming techniques can be developed. The issue of iPSC DNA integrity clearly highlights the crucial need of guidelines to define the acceptable level of genomic integrity of pluripotent stem cells for biomedical applications. We discuss here the main issues that such guidelines should address.

Published
2013
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