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Salmonella enterica is well known for its ability to survive and persist in low-moisture environments. Previous studies have indicated a link between the initial cell level and the population of Salmonella that survives after desiccation and subsequent storage; however, how the initial cell concentration affects survival is unknown. This study was conducted to examine this phenomenon and to determine whether it occurs in other microorganisms, specifically Shiga toxigenic Escherichia coli (STEC) and Enterococcus faecium. Salmonella, STEC, and E. faecium were grown as sessile cells on Trypticase soy agar with yeast extract (TSAYE) and harvested in buffered peptone water (BPW). To determine recovery at different initial cell levels, cultures were diluted to 9, 7, and 5 log CFU/mL and applied to filters. Filters were dried for 24 h and then stored for 28 days at 25°C and 33% relative humidity. During storage, cells were recovered from filters with BPW and cultivated on TSAYE. Recovery of both Salmonella and E. coli, but not E. faecium, was nonproportional. Lower initial populations were less viable after 24 h of desiccation; ≥10 log CFU/mL was recovered when 11 log CFU/mL was desiccated, but < 3 log CFU/mL was recovered when 5 log CFU/mL was desiccated. Once dried, persistence did not appear affected by initial cell concentration. When inactivated (heat-treated) cells were added to the diluent, recovery of Salmonella was proportional with respect to the initial cell level. To further examine the response to desiccation, Salmonella was diluted in BPW containing 1 of 11 test cell components related to quorum sensing or known to affect desiccation resistance to assess recovery and persistence. Of the 11 additions, only cell debris fractions, cell-free extract, and peptidoglycan improved recovery of Salmonella. Desiccation survival appears related to cell wall components; however, the exact mechanism affecting survival remains unknown. Salmonella and E. coli desiccation survival was dependent on initial levels. E. faecium recovery after desiccation was linear. Peptidoglycan and cell fractions improved desiccation resistance of Salmonella. [ABSTRACT FROM AUTHOR]

Infective Haemonchus contortus larvae (L3s) are able to protect themselves from desiccation. To explore the molecular mechanisms of desiccation survival, mRNA differential display RT-PCR was used to screen differentially expressed genes in L3s upon desiccation, followed by RNAi experiments to define gene functions. In this, 58 differentially expressed transcripts were obtained. Among these, the BF-U01A and CH-U02A fragments represent genes with the highest identity percentage in bioinformatic analysis. They were named Hc-ubq and Hc-gst based on their respective homologous ubiquitin in Caenorhabditis elegans and glutathione S-transferase in H. contortus . Quantitive RT-PCR results indicated that they were both up-regulated in desiccated L3s. Hc-ubq and Hc-gst RNAi in H. contortus showed reduced survival rate of L3s, with unchanged locomotion behavior. Homologous Ce-ubq-2 and Ce-gst-7 RNAi in C. elegans also displayed higher larval death rate. These results suggest that ubq and gst may play important roles in nematode desiccation tolerance. Our study analyzed desiccation resistance related genes in H. contortus L3s, and revealed significant research implications on the mechanisms behind nematode desiccation survival. [ABSTRACT FROM AUTHOR]

Fishing activities strongly contribute to biological invasions in freshwaters. The purpose of this study was to investigate the potential risks of live bait capture using dip nets and of crayfish trapping as vectors for invasive freshwater macrofauna dispersal. In the Tagus river basin (Portugal), where both activities are common, we evaluated the probability of capture and the electivity of the local aquatic macrofauna according to the method used. During the compulsory removal of the invasive species captured we also quantified fish desiccation survival capacities. We found, for both vectors, that the species exhibiting the highest probability of capture and the highest electivity were invasive, respectively, Gambusia holbrooki and Crangonyx pseudogracilis with the dip net, Procambarus clarkii and several invasive species with special relevance for Ameiurus melas with the crayfish trapping. Moreover, the desiccation survival capacities, of all invasive fishes analyzed, are compatible with long distance dispersal out of water, with special relevance to G. holbrooki . This study demonstrates that fishing activities contribute to long-distance dispersal of invasive fauna. Therefore, according to our findings, it is important to update the fishing regulation and simultaneously to raise fishermen awareness of this problem. [ABSTRACT FROM AUTHOR]

Osmotolerance is the ability to grow in an environment with a high osmotic pressure. In this study we compared the physiological parameters and tolerance to osmotic and non-osmotic stresses of three osmotolerant yeast species, Debaryomyces hansenii, Pichia farinosa ( sorbitophila) and Zygosaccharomyces rouxii, with those of wild-type Saccharomyces cerevisiae. Although the osmotolerant species did not differ significantly in their basic parameters, such as cell size or growth capacity, they had different abilities to survive anhydrobiosis, potassium limitation or the presence of toxic cationic drugs. When their osmotolerance was compared, the results revealed that some of the species isolated as sugar/polyol-tolerant (e.g. P. farinosa) are also highly tolerant to salts and, vice versa, some strains isolated from an environment with high concentration of salt (e.g. Z. rouxii ATCC 42981) tolerate high concentrations of sugars. None of the tested strains and species was osmophilic. Taken together, our results showed that P. farinosa ( sorbitophila) is the most robust species when coping with various stresses, while Z. rouxii CBS 732, although osmotolerant in general, is not specifically salt-tolerant and is quite sensitive to most of the tested stress conditions. Copyright © 2014 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

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Abstract: The foodborne bacterial pathogen, Listeria monocytogenes, commonly contaminates foods during processing, where the microorganisms are potentially subjected to low relative humidity (RH) conditions for extended periods of time. The objective of this study was to examine survival during desiccation (43% RH and 15°C) of biofilm L. monocytogenes N53-1 cells on stainless steel coupons and to assess subsequent transfer to salmon products. Formation of static biofilm (2days at 100% RH and 15°C) prior to desiccation for 23days significantly (P <0.05) improved survival of cells desiccated in initial low salt concentrations (0.5%) compared to the survival for non-biofilm cells also desiccated in low salt, indicating the protective effect of the biofilm matrix. Osmoadaptation of cells in 5% NaCl before formation of the static biofilm significantly (P <0.05) increased long-term desiccation survival (49days) irrespectively of the initial salt levels (0.5% and 5% NaCl). The efficiency of transfer (EOT) of desiccated biofilm cells was significantly (P <0.05) lower than EOTs for desiccated non-biofilm bacteria, however, as biofilm formation enhanced desiccation survival more bacteria were still transferred to smoked and fresh salmon. In conclusion, the current work shows the protective effect of biofilm formation, salt and osmoadaptation on the desiccation survival of L. monocytogenes, which in turn increases the potential for cross-contamination during food processing. [Copyright &y& Elsevier]