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To better understand the limitations of dormancy on Odontomyia Meigen, 1803 (Diptera: Stratiomyidae) survival and body condition, we conducted a series of laboratory-based microcosm experiments. We found that Odontomyia larvae can survive at least two extended dry periods (≥30 days) through dormancy. The presence of moisture in the substrate during dormancy is not necessary to survive and had no effect on survival or larval body mass in our experiments. Energy and bodily water loss likely limit survival in dormant stages. Although these results suggest Odontomyia are fairly resistant to drying, more data is needed on body mass, pupation, and reproductive success across hydroregimes. The more we understand about the survival limitations of rock pool aquatic invertebrates, the more successful conservation efforts will be for their unique aquatic biota. [ABSTRACT FROM AUTHOR]

Desert strains of the genus Chroococcidiopsis are among the most desiccation-resistant cyanobacteria capable of anhydrobiosis. The accumulation of two sugars, sucrose and trehalose, facilitates the entrance of anhydrobiotes into a reversible state of dormancy by stabilizing cellular components upon water removal. This study aimed to evaluate, at the atomistic level, the role of trehalose in desiccation resistance by using as a model system the 30S ribosomal subunit of the desert cyanobacterium Chroococcidiopsis sp. 029. Molecular dynamic simulations provided atomistic evidence regarding its protective role on the 30S molecular structure. Trehalose forms an enveloping shell around the ribosomal subunit and stabilizes the structures through a network of direct interactions. The simulation confirmed that trehalose actively interacts with the 30S ribosomal subunit and that, by replacing water molecules, it ensures ribosomal structural integrity during desiccation, thus enabling protein synthesis to be carried out upon rehydration. [ABSTRACT FROM AUTHOR]

Ostracods have frequently been reported as part of the active faunas in the wet phase of rice fields, but knowledge on the dormant ostracods during the dry period of the rice cycle has been limited, hindering a comprehensive understanding of their adaptation to the rice field environment. In this study, we record 11 dormant ostracod species by incubating soil samples from six desiccated rice fields in Yunnan Province of southwestern China. The dormant ostracod community was dominated by species of the family Cyprididae, accompanied by members of Candonidae, Ilyocyprididae, and Notodromadidae. Our data provide unequivocal evidence for the presence of dormant ostracods from all the four families of the superfamily Cypridoidea in desiccated rice fields, verifying that these ostracods adapt to the rice fields by desiccation-resistant stage. Considering the usually shallow penetration depth of ostracods, the frequent discoveries of dormant ostracods in 2‒4 cm depth in the soil profile were probably the result of ploughing. Despite overlap in species composition, the dormant ostracod community differs from the active community sampled at the same sites more than four years ago. This suggests temporal changes of faunal composition, although the ecological memory of the dormant community containing multiple generations and the different seasonal windows represented by the two types of communities should also be considered. The emergence of adults within the first two weeks of the incubation experiment suggests the presence of dormant instars in the soil rather than resting eggs alone. [ABSTRACT FROM AUTHOR]

Garcinia xanthochymus is a climax tree species growing in the Xishuangbanna tropical rainforests, which possesses a unique adaptation mechanism to the tropical monsoon climate, i.e., its seeds mature at the end of the rainy season and shed, mostly do not germinate until next rainy season, distinctly differing from most other climax species there. This study investigated the morphological structure, desiccation tolerance, and germination characteristics of G. xanthochymus seeds to understand their cytological basis and ecological adaptation. It was found that G. xanthochymus seeds germinate when incubated at temperatures between 20 and 35 °C, with a thermal optimum of 30 °C, but have a short period of dormancy (> 40 days even at 30 °C). The seeds were intolerant to desiccation, and completely lost viability when desiccated to 25% miosture content (fresh weight basis), exhibiting typical recalcitrant seed traits. However, they also possessed strong desiccation resistance, maintaining approximately 33% misture content and 50% viability even after 20 days of silica gel drying. Until germination, G. xanthochymus seeds did not exhibit visible structural differentiation. They are composed of a cluster of uniformly shaped, shallowly differentiated parenchymal cells possessing embryonic and storage functions. Thus, they germinate totipotently and can regenerate into seedlings even after being subjected to various cutting, which protects them from animal grazing. This explains how G. xanthochymus seeds withstand biotic and abiotic stresses during the dry season in Xishuangbanna, meanwhile implys that G. xanthochymus is more dependent on the forest environment and more sensitive to habitat changes. [ABSTRACT FROM AUTHOR]

Cuticular hydrocarbons (CHCs) of Drosophila ananassae (Doleschall) was characterized and identified using gas chromatography and mass spectrometry (GC-MS) analysis. A high % of methyl-branched alkanes were identified in all inversion (2LA, 3LA and 2LA+3LA) and inversion-free strains followed by linear alkanes and alkenes. The present work unfolds the significant patterns of variations in the isomeric forms of methylated alkanes between the inversion and inversion free strains (F-46.6; df-3, p 0.005), and non-significant between the sex (F-2.14; df-1, p 0.2394). But in linear alkanes shows significant variation between the inversion strains (F-30.49; df-3, p 0.009) and between the male and female (F-115.45; df-1, p 0.001) was observed. In particular there is a significant correlation between the chromosomal inversion and synthesis of CHCs in D. ananassae. Unique blend of CHCs in Drosophila performs dual role as desiccation resistance and act as chemical signalling molecule. Linear alkanes are majorly involved in desiccation resistance but in methyl- branched CHCs length variation is a key determinant of desiccation resistance. Presence of longer methyl- branched alkanes and higher desiccation resistance, shorter the carbon chain length act as a signalling molecules. The current study revealed the influence of chromosomal inversion on the cuticular hydrocarbon profile in D. ananassae. [ABSTRACT FROM AUTHOR]

This study conducted diallel hybridization experiments on three excellent populations of Procambarus clarkii, namely Gehu, Jiangsu; Jianhu, Jiangsu; and Wuhu, Anhui, with high genetic diversity and fast growth. Six hybrid groups were obtained ［Gehu ♀ × Jianhu ♂ （GJ）, Jianhu ♀ × Gehu ♂ （JG）, Gehu ♀ × Wuhu ♂ （GW）, Wuhu ♀ × Gehu ♂ （WG）, Jianhu ♀ × Wuhu ♂ （JW）, Wuhu ♀ × Jianhu ♂ （WJ）］, and three self crossing groups ［ Gehu ♀ × Gehu ♂（ GG）, Jianhu ♀ × Jianhu ♂（ JJ）, Wuhu ♀ × Wuhu ♂ （WW）］. A comparative analysis was conducted on the growth performance and ecological adaptability of 9 combinations. The results showed that: （1） During the breeding cycle, the weight gain and weight gain rate of both male and female individuals in the WW and JJ combinations were significantly higher than those in the other combinations （P<0. 05）; Except for GJ and JG, the weight gain and growth rate of male individuals in all combinations were significantly higher than those in females. From March to May, no heterosis was observed in the growth traits of each hybrid group; （2） With the increase of breeding time, there were significant changes in the length/body length of the head and chest armor, as well as the length/body length of the abdomen in the 9 populations of crayfish. The length/body length of the head and chest armor gradually increased, while the length/body length of the abdomen gradually decreased. In May, there was differentiation between male and female individuals, and the proportion of female abdomen length was significantly higher than that of male. The ratio of head to chest armor width/body length, abdominal width/body length, and tail segment length/body length only showed a significant increase in April; （3） After 24 hours of dry dew, each group of shrimp began to die, and at 48 hours, the survival rate of each group showed significant differentiation. At 48 hours, the survival rate of the WW combination was significantly higher than that of the other combinations. The GJ and JG cross groups showed heterosis in terms of dry dew tolerance. This study found that the mixed germplasm of crayfish limited the heterosis of growth traits to a certain extent, but showed heterosis in stress resistance. Proper hybridization and re-selection are important breeding strategies. The growth and abdominal length ratio of crayfish showed obvious male and female dimorphism, and single sex breeding is also an important direction for future research. [ABSTRACT FROM AUTHOR]

Free‐living organisms frequently encounter unfavorable abiotic environmental factors. Those who adapt and cope with sudden changes in the external environment survive. Desiccation is one of the most common and frequently encountered stresses in nature. On the contrary, ionizing radiations are limited to high local concentrations of naturally occurring radioactive materials and related anthropogenic activities. Yet, resistance to high doses of ionizing radiation is evident across the tree of life. The evolution of desiccation resistance has been linked to the evolution of ionizing radiation resistance, although, evidence to support the idea that the evolution of desiccation tolerance is a necessary precursor to ionizing radiation resistance is lacking. Moreover, the presence of radioresistance in hyperthermophiles suggests multiple paths lead to radiation resistance. In this minireview, we focus on the molecular aspects of damage dynamics and damage response pathways comprising protective and restorative functions with a definitive survival advantage, to explore the serendipitous genesis of ionizing radiation resistance. [ABSTRACT FROM AUTHOR]

Cronobacter sakazakii, an important Gram‐negative foodborne pathogen, can cause neonatal meningitis and sepsis with high rates of infection and death. Gene ESA_RS15745 encodes a sugar transporter protein, which is not only essential for osmotic pressure maintenance during bacterial growth and reproduction but also associated with their desiccation tolerance, motility, and biofilm formation. Here, a mutant strain of ESA_RS15745 (ΔESA_RS15745) and the complementation strain (cpESA_RS15745) were constructed using a suicide vector knockout and gene complementation. ΔESA_RS15745 was found to have a decrease in its ability to transport maltose and trehalose and resist desiccation, whereas an increase in the ability of motility and biofilm formation, implying that ESA_RS15745 may positively regulate sugar transport and desiccation tolerance and negatively regulate motility and biofilm formation. To further investigate the molecular mechanisms underlying the function of related genes, RNA‐seq was performed to explore the differentially expressed genes in the mutants. RNA‐seq results showed the upregulation of 114 genes (mainly including those regulating chemotaxis and flagellar motility) and the downregulation of 22 genes (mainly including those regulating sugar transport). qRT‐PCR analysis supported the RNA‐seq results and showed that ESA_RS15745 may influence the dehydration tolerance though decreasing the intracellular trehalose content and negatively regulate the motility though the chemotactic signaling pathway. In addition, the biofilm formation of C. sakazakii should also be speculated to negatively regulate by ESA_RS15745 by consuming the extracellular carbohydrates concentration and then downregulating the intracellular cyclic diguanosine monophosphate. This study offers a reference for comprehending the molecular mechanism of gene ESA_RS15745 in C. sakazakii. [ABSTRACT FROM AUTHOR]

Desert strains of the genus Chroococcidiopsis are among the most desiccation-resistant cyanobacteria capable of anhydrobiosis. The accumulation of two sugars, sucrose and trehalose, facilitates the entrance of anhydrobiotes into a reversible state of dormancy by stabilizing cellular components upon water removal. This study aimed to evaluate, at the atomistic level, the role of trehalose in desiccation resistance by using as a model system the 30S ribosomal subunit of the desert cyanobacterium Chroococcidiopsis sp. 029. Molecular dynamic simulations provided atomistic evidence regarding its protective role on the 30S molecular structure. Trehalose forms an enveloping shell around the ribosomal subunit and stabilizes the structures through a network of direct interactions. The simulation confirmed that trehalose actively interacts with the 30S ribosomal subunit and that, by replacing water molecules, it ensures ribosomal structural integrity during desiccation, thus enabling protein synthesis to be carried out upon rehydration.

Drosophila larvae and pupae are vulnerable to seasonal abiotic stressors such as humidity and temperature. In wild low-humidity habitats, desiccation stress can occur as Drosophila larvae forsake wet food in search of a drier pupation site. Henceforth, the hypothesis that developmental humidity impacts pupation height, affecting larval and pupae water balance and fitness-related traits, was examined. Accordingly , warm-adapted Drosophilid - Zaprionus indianus from two seasons were reared under season-specific simulated conditions, with significantly varying relative humidity (summer RH: 40%; rainy RH: 80%), but nearly identical temperatures. A trade-off between pupation height and developmental humidity was observed. Drier summer conditions lead to pupae wandering farther from drier glass surfaces, resulting in higher pupation height (17.3 cm) while rainy pupae prefer wet food, resulting in lower pupation height (7.12 cm). Additionally, density-dependent pupation height was developmental humidity-specific, with most rainy-season pupae pupated on wetter food, while dry summer pupae pupated on glass surfaces or cotton. Nevertheless, flies from far pupation exhibited greater desiccation resistance, fecundity, and copulation duration than those from near pupation. The cuticular lipid mass of larvae and pupae was higher during far-than-near pupation, indicating decreased water loss rates compared to near-pupation. Finally, pupae eclosion (%) was unaffected by greater humidity (85%) in either season. Still, it considerably decreased at lower humidity (RH: 0% and 38%) for rainy pupae, further supporting the selection of low-humidity desiccation resistance in pupae. In conclusion, low humidity is crucial for survival of pre-adult stages of Zaprionus indianus under desiccation stress and for preference of pupation site. [Display omitted] • Drosophila larvae and pupae are vulnerable to humidity and temperature. • Zaprionus indianus showed a trade-off between pupation height and developmental humidity. • Density-dependent pupation height was developmental humidity-specific. • Dry summer increased pupation height, while wet rains lower pupation height. • Pupae eclosion (%) significantly decreased at lower humidity levels, indicating the importance of low-humidity desiccation resistance in pupae selection. [ABSTRACT FROM AUTHOR]

Environmental stress is one of the important causes of biological dispersal. At the same time, the process of dispersal itself can incur and/or increase susceptibility to stress for the dispersing individuals. Therefore, in principle, stress can serve as both a cause and a cost of dispersal. We studied these potentially contrasting roles of a key environmental stress (desiccation) using Drosophila melanogaster. By modulating water and rest availability, we asked whether (a) dispersers are individuals that are more susceptible to desiccation stress, (b) dispersers pay a cost in terms of reduced resistance to desiccation stress, (c) dispersal evolution alters the desiccation cost of dispersal, and (d) females pay a reproductive cost of dispersal. We found that desiccation was a clear cause of dispersal in both sexes, as both male and female dispersal propensity increased with increasing duration of desiccation. However, the desiccation cost of dispersal was male biased, a trend unaffected by dispersal evolution. Instead, females paid a fecundity cost of dispersal. We discuss the complex relationship between desiccation and dispersal, which can lead to both positive and negative associations. Furthermore, the sex differences highlighted here may translate into differences in movement patterns, thereby giving rise to sex-biased dispersal patterns. [ABSTRACT FROM AUTHOR]

Cuticular hydrocarbons (CHCs) are diverse in insects, and include variable classes of cuticular lipids, contributing to waterproofing for insects under desiccation environments. However, this waterproofing function of CHCs is still not well characterized in aphids. In this study, we compared CHC profiles for desiccation-resistant and nonresistant genotypes of the grain aphid, Sitobion avenae (Fabricius), in responses to desiccation. Our result showed that a total of 27 CHCs were detected in S. avenae, and linear alkanes (e.g., n-C29) were found to be the predominant components. Long-chain monomethyl alkanes were found to associate closely with water loss rates in S. avenae in most cases. Resistant genotypes of both wing morphs had higher contents of short-chain n-alkanes under control than nonresistant genotypes, showing the importance of short-chain n-alkanes in constitutive desiccation resistance. Among these, n-C25 might provide a CHC signature to distinguish between desiccation-resistant and nonresistant individuals. Compared with linear alkanes, methyl-branched CHCs appeared to display higher plasticity in rapid responses to desiccation, especially for 2-MeC26, implying that methyl-branched CHCs could be more sensitive to desiccation, and play more important roles in induced desiccation-resistance. Thus, both constitutive and induced CHCs (linear or methyl-branched) can contribute to adaptive responses of S. avenae populations under desiccation environments. Our results provide substantial evidence for adaptive changes of desiccation resistance and associated CHCs in S. avenae, and have significant implications for aphid evolution and management in the context of global climate change. [ABSTRACT FROM AUTHOR]

Zoologists have long tried to culture the limnoterrestrial heterotardigrades associated with bryophytes and lichens without success. By carefully observing heterotardigrades in the genera Echiniscus, Pseudechiniscus, and Viridiscus over many months, we determined that these organisms feed on chloroplasts and cytoplasm from the cells of moss (typically moss protonema), and on the single‐celled green algae associated with the moss (typically Chlorella vulgaris). We also determined that the cryptogams they associate with, and hence the heterotardigrades themselves, spend more time in a dried state than do most eutardigrades, which are more easily cultured. Taking these observations into account, we varied food, water, and desiccation cycle conditions with the aim of developing a viable culturing protocol for heterotardigrades. We used this experimentally derived protocol to maintain laboratory cultures of the tardigrades Echiniscus sp., Pseudechiniscus aff. P. ramazzotti and Viridiscus viridianus, which regularly produced new eggs and hatchlings. Both moss and algae from their natural habitats were used as food sources, and a small layer of rain or spring water was added every morning and allowed to partially evaporate overnight. Furthermore, the organisms were more likely to lay eggs on a dense mat of moss protonema, grown by inserting tips of moss branches into a solidified KCM–agar medium. The medium also provided a walking substrate for the tardigrades, and possibly a source of cations. Crucially, the cultures were allowed to dry out completely every 10 days for a period of at least 3 days. Moss in the culture dish significantly improved the chances of the tardigrades coming out of their desiccation‐resistant states successfully. The ability to culture heterotardigrades makes these organisms available for modern genomics and other studies with implications for understanding desiccation resistance mechanisms beyond those studied in a few model eutardigrade species. [ABSTRACT FROM AUTHOR]

1. Protection against desiccation and chemical communication are two fundamental functions of cuticular hydrocarbons (CHCs) in insects. In the parasitoid jewel wasp Nasonia vitripennis (Walker), characterised by a cosmopolitan distribution through largely different environments, CHCs function as universally recognised female sex pheromones. However, CHC uniformity as basis for sexual recognition may conflict with the desiccation protection function, expected to display considerable flexibility through adaptation to different environmental conditions. 2. We compared male and female CHC profiles of N. vitripennis across a wide latitudinal gradient in Europe and correlated their CHC variation with climatic factors associated with desiccation. Additionally, we tested male mate discrimination behaviour between populations to detect potential variations in female sexual attractiveness. 3. Results did not conform to the general expectation that longer, straight‐chain CHCs occur in higher proportions in warmer and drier climates. Instead, unexpected environmental correlations of intermediate chain‐length CHCs (C31) were found exclusively in females, potentially reflecting the different life histories of the sexes in N. vitripennis. 4. Furthermore, we found no indication of population‐specific male mate preference, confirming the stability of female sexual attractiveness, likely conveyed through their CHC profiles. C31 mono‐ and C33 di‐methyl‐branched alkanes were consistently and most strongly associated with sexual dimorphism, suggesting their potential role in encoding the female‐specific sexual signalling function. 5. Our study sheds light on how both adaptive flexibility and conserved sexual attractiveness can potentially be integrated and encoded in CHC profiles of N. vitripennis females across a wide distribution range in Europe. [ABSTRACT FROM AUTHOR]

Abstract Background The highly polyphagous Queensland fruit fly (Bactrocera tryoni Froggatt) expanded its range substantially during the twentieth century and is now the most economically important insect pest of Australian horticulture, prompting intensive efforts to develop a Sterile Insect Technique (SIT) control program. Using a “common garden” approach, we have screened for natural genetic variation in key environmental fitness traits among populations from across the geographic range of this species and monitored changes in those traits induced during domestication. Results Significant variation was detected between the populations for heat, desiccation and starvation resistance and wing length (as a measure of body size). Desiccation resistance was correlated with both starvation resistance and wing length. Bioassay data for three resampled populations indicate that much of the variation in desiccation resistance reflects persistent, inherited differences among the populations. No latitudinal cline was detected for any of the traits and only weak correlations were found with climatic variables for heat resistance and wing length. All three stress resistance phenotypes and wing length changed significantly in certain populations with ongoing domestication but there was also a strong population by domestication interaction effect for each trait. Conclusions Ecotypic variation in heat, starvation and desiccation resistance was detected in Australian Qfly populations, and these stress resistances diminished rapidly during domestication. Our results indicate a need to select source populations for SIT strains which have relatively high climatic stress resistance and to minimise loss of that resistance during domestication.

Insects have the ability to readily adapt to changes in environmental conditions, however the strength of local environmental adaptation signals under divergent conditions and the occurrence of trait inertia after relaxation of selection, remains poorly understood, especially for traits of climate stress resistance (CSR) and their phenotypic plasticity. The strength of environmental adaptation signals depend on several selection pressures present in the local environment, while trait inertia often occurs when there is a weakening or removal of a source of selection. Here, using Drosophila melanogaster, we asked whether signals of adaptation in CSR traits (critical thermal limits, heat and chill survival and, desiccation and starvation resistance) persist after exposure to laboratory culture for different durations (two vs. ten generations) across four climatically distinct populations. We show that culture duration has large effects on CSR traits and can both amplify or dilute signals of local adaptation. Effects were however dependent upon interactions between the source population, acclimation (adult acclimation at either 18 °C, 23 °C or 28 °C) conditions and the sex of the flies. Trait plasticity is markedly affected by the interaction between the source population, the specific acclimation conditions employed, and the duration in the laboratory. Therefore, a complex matrix of dynamic CSR trait responses is shown in space and time. Given these strong interaction effects, ‘snapshot’ estimates of environmental adaptation can result in misleading conclusions about the fitness consequences of climate variability.

ABSTRACT Although aedine eggs are resistant to desiccation, little is known about this trait in Haemagogus species. In this study, we assessed the desiccation resistance of Hg. spegazzinii eggs. The eggs were collected using ovitraps over a period of one week in March 2020. The positive pallets were stored until experimentation and immersed in a solution of water and yeast for larval hatching and subsequent species identification. The emerged adults were morphologically identified as Hg. spegazzinii. Because of color variations, adults were classified into three groups. The desiccation resistance of the eggs collected was measured for 380 days. Desiccation resistance is likely the result of selection mechanisms responsible for maintaining populations of the species in the seasonally dry tropical forest (Caatinga) in the Brazilian semiarid region. Variations in scale coloration can represent either individual variations or new species.

This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus). A total of 540 (body weight of 0.41 ± 0.05) crayfish were randomly divided into six groups for a feeding experiment. Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg−1 were prepared to feed juvenile red claw crayfish for 8 weeks. The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1). Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6 were significantly higher than those of the control group. The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control. The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group. However, acid phosphatase (ACP) activity was not significantly different among all experimental groups. The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels. Compared with the control group, supplementation of 5–15 g kg−1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA. In addition, supplementation of 5–15 g kg−1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress. These results suggested that supplementation of 5–15 g kg−1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish. • Dietary trehalose improved growth performance and non-specific immunity of crayfish. • Dietary trehalose increased muscle crude protein content and reduced lipid content. • Dietary trehalose increased trehalose content in hepatopancreas and intestine. • Supplementation of 5–15 g kg−1 trehalose improved the resistance to desiccation. [ABSTRACT FROM AUTHOR]

Extremophilic prokaryotes live under harsh environmental conditions which require far-reaching cellular adaptations. The acquisition of novel genetic information via natural transformation plays an important role in bacterial adaptation. This mode of DNA transfer permits the transfer of genetic information between microorganisms of distant evolutionary lineages and even between members of different domains. This phenomenon, known as horizontal gene transfer (HGT), significantly contributes to genome plasticity over evolutionary history and is a driving force for the spread of fitness-enhancing functions including virulence genes and antibiotic resistances. In particular, HGT has played an important role for adaptation of bacteria to extreme environments. Here, we present a survey of the natural transformation systems in bacteria that live under extreme conditions: the thermophile Thermus thermophilus and two desiccation-resistant members of the genus Acinetobacter such as Acinetobacter baylyi and Acinetobacter baumannii. The latter is an opportunistic pathogen and has become a world-wide threat in health-care institutions. We highlight conserved and unique features of the DNA transporter in Thermus and Acinetobacter and present tentative models of both systems. The structure and function of both DNA transporter are described and the mechanism of DNA uptake is discussed. [ABSTRACT FROM AUTHOR]

Insects, similarly to other small terrestrial invertebrates, are particularly susceptible to climatic stress. Physiological adjustments to cope with the environment (i.e., acclimation) together with genetic makeup eventually determine the tolerance of a species to climatic extremes, and constrain its distribution. Temperature and desiccation resistance in insects are both conditioned by acclimation and may be interconnected, particularly for species inhabiting xeric environments. We determined the effect of temperature acclimation on desiccation resistance of the peach fruit fly (Bactrocera zonata, Tephritidae) – an invasive, polyphagous pest, currently spreading through both xeric and mesic environments in Africa and the Eurasian continent. Following acclimation at three constant temperatures (20, 25, and 30°C), the survival of adult flies deprived of food and water was monitored in extreme dry and humid conditions (90% relative humidity, respectively). We found that flies acclimated at higher temperatures were significantly heavier, and contained more lipids and protein. Acclimation temperature significantly and similarly affected the survival of males and females at both high and low humidity conditions. In both cases, flies maintained at 30°C survived longer compared to 20 and 25°C – habituated counterparts. Regardless of the effect of acclimation temperature on survival, overall life expectancy was significantly shortened when flies were assayed under desiccating conditions. Additionally, our experiments indicate no significant difference in survival patterns between males and females, and that acclimation temperature had similar effects after both short (5–10 days) and long (11–20 days) acclimation periods. We conclude that acclimation at 30°C prolongs the survival of B. zonata, regardless of ambient humidity levels. Temperature probably affected survival through modulating feeding and metabolism, allowing for accumulation of larger energetic reserves, which in turn, promoted a greater ability to resist starvation, and possibly desiccation as well. Our study set the grounds for understanding the phenotypic plasticity of B. zonata from the hydric perspective, and for further evaluating the invasion potential of this pest.

Insects, similarly to other small terrestrial invertebrates, are particularly susceptible to climatic stress. Physiological adjustments to cope with the environment (i.e., acclimation) together with genetic makeup eventually determine the tolerance of a species to climatic extremes, and constrain its distribution. Temperature and desiccation resistance in insects are both conditioned by acclimation and may be interconnected, particularly for species inhabiting xeric environments. We determined the effect of temperature acclimation on desiccation resistance of the peach fruit fly (Bactrocera zonata , Tephritidae) – an invasive, polyphagous pest, currently spreading through both xeric and mesic environments in Africa and the Eurasian continent. Following acclimation at three constant temperatures (20, 25, and 30°C), the survival of adult flies deprived of food and water was monitored in extreme dry and humid conditions (<10 and >90% relative humidity, respectively). We found that flies acclimated at higher temperatures were significantly heavier, and contained more lipids and protein. Acclimation temperature significantly and similarly affected the survival of males and females at both high and low humidity conditions. In both cases, flies maintained at 30°C survived longer compared to 20 and 25°C – habituated counterparts. Regardless of the effect of acclimation temperature on survival, overall life expectancy was significantly shortened when flies were assayed under desiccating conditions. Additionally, our experiments indicate no significant difference in survival patterns between males and females, and that acclimation temperature had similar effects after both short (5–10 days) and long (11–20 days) acclimation periods. We conclude that acclimation at 30°C prolongs the survival of B. zonata , regardless of ambient humidity levels. Temperature probably affected survival through modulating feeding and metabolism, allowing for accumulation of larger energetic reserves, which in turn, promoted a greater ability to resist starvation, and possibly desiccation as well. Our study set the grounds for understanding the phenotypic plasticity of B. zonata from the hydric perspective, and for further evaluating the invasion potential of this pest. [ABSTRACT FROM AUTHOR]

Background and aims - Tracheophyte leaf morphology is well studied but it is unclear if the findings generalize to poikilohydric plants. We tested combinations of hypotheses to determine if microhabitat characteristics, including light exposure, moisture availability, and substrate slope, controlled for morphological differences between upright and prostrate growth forms, affect phyllid surface area and costa length of mosses. Material and methods - We quantified mean phyllid surface-area and costa lengths for four replicates of 38 moss species from Alabama. Phylogenetic comparative methods that model adaptation were used to evaluate the relative evidence for each hypothesis using information criteria. To further explore mechanistic explanations involving substrate slope, we tested whether mosses on vertical substrates differed from those on horizontal substrates in the average amount of water-retaining, nutrient-rich litter they accumulated. Key results - Substrate slope and growth form combined were the best predictors of phyllid surface area. Mosses growing on vertical substrates exhibited smaller phyllid surface area for both growth forms. Although growth form and phyllid length best explained costa length variation, a more complex model including substrate slope performed nearly as well. Within the prostrate growth forms, species growing on vertical substrates exhibit longer relative costa than those on horizontal substrates. We also estimated rapid rates of adaptation for both traits. Conclusion - The smaller phyllid surface area of both upright and prostrate growth forms is possibly an adaptive response to reduced habitat moisture-retention or nutrient quality that vertical substrates offer. The longer costa lengths of prostrate mosses growing on vertical surfaces relative to prostrate mosses on horizontal surfaces, possibly make up for the decreased ability of smaller phyllids to rapidly reabsorb water when it is available. Further work is required to determine if it is truly substrate slope itself that matters or other variables associated with the differences in slope, and to determine how general this phenomenon is. [ABSTRACT FROM AUTHOR]

Cuticular hydrocarbons form a barrier that protects terrestrial insects from water loss via the epicuticle. Lipophorin loads and transports lipids, including hydrocarbons, from one tissue to another. In some insects, the lipophorin receptor (LpR), which binds to lipophorin and accepts its lipid cargo, is essential for female fecundity because it mediates the incorporation of lipophorin by developing oocytes. However, it is unclear whether LpR is involved in the accumulation of cuticular hydrocarbons and its precise role in aphid reproduction remains unknown. We herein present the results of our molecular characterization, phylogenetic analysis, and functional annotation of the pea aphid (Acyrthosiphon pisum) LpR gene (ApLpR). This gene was transcribed throughout the A. pisum life cycle, but especially during the embryonic stage and in the abdominal cuticle. Furthermore, we optimized the RHA interference (RNAi) parameters by determining the ideal dose and duration for gene silencing in the pea aphid. We observed that the RNAi‐based ApLpR suppression significantly decreased the internal and cuticular hydrocarbon contents as well as adult fecundity. Additionally, a deficiency in cuticular hydrocarbons increased the susceptibility of aphids to desiccation stress, with decreased survival rates under simulated drought conditions. Moreover, ApLpR expression levels significantly increased in response to the desiccation treatment. These results confirm that ApLpR is involved in transporting hydrocarbons and protecting aphids from desiccation stress. Furthermore, this gene is vital for aphid reproduction. Therefore, the ApLpR gene of A. pisum may be a novel RNAi target relevant for insect pest management. [ABSTRACT FROM AUTHOR]

Cuticular hydrocarbons (CHCs) are important, multi-function components of the insect epicuticle. In Drosophila spp., CHCs provide protection from desiccation and serve as semiochemicals for both intra- and interspecific communication. We developed a non-lethal method for the modification of Drosophila CHCs profiles through the exposure of live insects to a high dose of ozone gas (~ 45,000 ppm). Drosophila suzukii that were treated with ozone showed a 1.63–3.10 fold reduction in unsaturated hydrocarbons with these CHCs shown to regenerate over 108 h. Changes in CHCs were correlated with significantly reduced desiccation resistance in both male and female D. suzukii at one h after ozone treatment. Interestingly, individuals treated with ozone showed increased desiccation resistance in comparison to controls at 108 h after ozone treatment. The methodology reported in this paper provides a novel approach to investigate the biosynthesis and functions of CHCs during the lifespan of an insect. [ABSTRACT FROM AUTHOR]

• Warmer temperatures or drier conditions accelerated bumble bee water loss. • Warmer temperatures, but not drier conditions, resulted in earlier mortality. • Bumble bees had less water in their body at mortality under low humidity. • Larger bees could withstand more stressful conditions. • Colonies varied in their water balance traits. Ongoing climate change has increased temperatures and the frequency of droughts in many parts of the world, potentially intensifying the desiccation risk for insects. Because resisting desiccation becomes more difficult at higher temperatures and lower humidity, avoiding water loss is a key challenge facing terrestrial insects. However, few studies have examined the interactive effects of temperature and environmental humidity on desiccation resistance in insects. Such studies on bees (Hymenoptera: Apoidea: Anthophila) are especially rare, despite their ecological and economic importance. Here, we crossed temperature (20, 25, and 30 °C) with humidity (<5, 50, >95 % RH) manipulations and measured time to mortality, water loss rates, and the water content at mortality of bumble bees (Bombus impatiens). We found that both higher temperature and lower humidity increased water loss rates, while warmer temperatures reduced survival time and lower humidity decreased water content at mortality. Additionally, we observed large intraspecific variation in water balance traits between colonies, and larger individuals survived longer and could tolerate more water loss before mortality. This study raises important questions about the mechanisms underpinning water loss in bumble bees and suggests that frequent access to nectar may be especially important for bumble bees' water balance and survival in a warming and drying climate. [ABSTRACT FROM AUTHOR]

Abstract Background Aedes aegypti is a major disease vector in urban habitats, involved in the transmission of dengue, chikungunya and Zika. Despite innumerous attempts to contain disease outbreaks, there are neither efficient vaccines nor definite vector control methods nowadays. In recent years, an innovative strategy to control arboviruses, which exploits the endosymbiotic bacterium Wolbachia pipientis, emerged with great expectations. The success of the method depends on many aspects, including Wolbachia’s cytoplasmic incompatibility and pathogen interference phenotypes, as well as its effect on host fitness. In this work, we investigated the influence the Wolbachia strain wMel exerts on embryo development and egg viability and speculate on its field release use. Methods Wild-type (Br or Rockefeller) and Wolbachia-harboring specimens (wMelBr) were blood-fed and submitted to synchronous egg laying for embryo development assays. Samples were analyzed for morphological markers, developmental endpoint and egg resistance to desiccation (ERD). Quiescent egg viability over time was also assessed. Results wMelBr samples completed embryogenesis 2–3 hours later than wild-type. This delay was also observed through the onset of both morphological and physiological markers, respectively by the moments of germband extension and ERD acquisition. Following the end of embryonic development, wMelBr eggs were slightly less resistant to desiccation and showed reduced viability levels, which rapidly decayed after 40 days into quiescence, from approximately 75% to virtually 0% in less than a month. Conclusions Our data revealed that the wMel strain of Wolbachia slightly delays embryogenesis and also affects egg quality, both through reduced viability and desiccation resistance. These findings suggest that, although embryonic fitness is somehow compromised by wMel infection, an efficient host reproductive manipulation through cytoplasmic incompatibility seems sufficient to overcome these effects in nature and promote bacterial invasion, as shown by successful ongoing field implementation.

Desiccation resistance is a key trait determining the distribution of aquatic insects, their potential for overland dispersal, and survival during drought periods in temporary waterbodies, as well as the spread of invasive species. Passive dispersal by waterbirds is considered to be a key process favouring the spread of invasive species and is more likely to occur for insects with eggs that are resistant to desiccation or gut passage.We investigated the hypothesis that the eggs of the alien boatman Trichocorixa verticalis verticalis are resistant to desiccation and digestion and thus able to disperse via waterbirds.We conducted experiments to test for egg resistance to desiccation in still air and under moving air conditions, to simulate bird flight. Oviposition on greater flamingo (Phoenicopterus roseus) legs and wetland plants was tested in the laboratory and in the field. Resistance of eggs to simulated gut passage was studied, as was the viability of eggs recovered from Eurasian coot (Fulica atra) droppings in the field.Only a fraction of eggs hatched after 8 hr of exposure to still air at 15°C (4% of exposed eggs), and after 2 hr exposure to air flow conditions at 20°C (8%). Oviposition on flamingo legs was confirmed in the laboratory but not in the field. However, oviposition rates were higher on plant stems than on flamingo legs. Digestion simulations showed very low resistance to chemical treatment and no resistance to scarification, and eggs recovered from coot droppings did not hatch.Our experiments demonstrated that T. v. verticalis cannot be considered to have eggs resistant to desiccation or gut passage. However, the dispersal of eggs on legs of flamingos or other waterbirds may occur as rare events. This epizoochory of eggs could potentially contribute to the expansion and gene flow of T. v. verticalis around the Mediterranean region. [ABSTRACT FROM AUTHOR]

For a period of 6-15 days prior to death, the fecundity and virility of Drosophila melanogaster fall significantly below those of same-aged flies that are not near death. It is likely that other aspects of physiology may decline during this period. This study attempts to document changes in two physiological characteristics prior to death: desiccation resistance and time-in-motion. Using individual fecundity estimates and previously described models, it is possible to accurately predict which flies in a population are near death at any given age; these flies are said to be in the "death spiral". In this study of approximately 7,600 females, we used cohort mortality data and individual fecundity estimates to dichotomize each of five replicate populations of same-aged D. melanogaster into "death spiral" and "non-spiral" groups. We then compared these groups for two physiological characteristics that decline during aging. We describe the statistical properties of a new multivariate test statistic that allows us to compare the desiccation resistance and time-in-motion for two populations chosen on the basis of their fecundity. This multivariate representation of the desiccation resistance and time-in-motion of spiral and non-spiral females was shown to be significantly different with the spiral females characterized by lower desiccation resistance and time spent in motion. Our results suggest that D. melanogaster may be used as a model organism to study physiological changes that occur when death is imminent.

Global warming and climate change are affecting many insect species in numerous ways. These species can develop diverse mechanisms as a response to variable environmental conditions. The rise in mean and extreme temperatures due to global warming and the importance of the population’s ability to adapt to temperature stress will further increase. In this study, we investigated thermal stress response, which is considered to be one of the crucial elements of population fitness and survival in fast-changing environments. The dynamics and variation of thermal stress resistance traits in D. subobscura flies originating from two natural populations sampled from different altitudes were analysed. Three different temperature regimes (25 °C, 19 °C, and 16 °C) were used for the F1 progeny from both localities to establish six experimental groups and investigate stress resistance traits: desiccation resistance, heat knock-down resistance, starvation resistance, and chill-coma recovery time. We detected that laboratory thermal conditions and population origin may have an effect on the analysed traits, and that sex also significantly influences stress resistance. Individuals from the lower altitude reared at higher temperatures show inferior resistance to thermal shock.

The adaptive significance of sympatric mitochondrial (mtDNA) variation and the role of selective mechanisms that maintain it are debated to this day. Isofemale lines of Drosophila obscura collected from four populations were backcrossed within populations to construct experimental lines, with all combinations of mtDNA Cyt b haplotypes and nuclear genetic backgrounds (nuDNA). Individuals of both sexes from these lines were then subjected to four fitness assays (desiccation resistance, developmental time, egg-to-adult viability and sex ratio) on two experimental temperatures to examine the role of temperature fluctuations and sex-specific selection, as well as the part that interactions between the two genomes play in shaping mtDNA variation. The results varied across populations and fitness components. In the majority of comparisons, they show that sympatric mitochondrial variants affect fitness. However, their effect should be examined in light of interactions with nuDNA, as mito-nuclear genotype was even more influential on fitness across all components. We found both sex-specific and temperature-specific differences in mitochondrial and mito-nuclear genotype ranks in all fitness components. The effect of temperature-specific selection was found to be more prominent, especially in desiccation resistance. From the results of different components tested, we can also infer that temperature-specific mito-nuclear interactions rather than sex-specific selection on mito-nuclear genotypes have a more substantial role in preserving mtDNA variation in this model species.

Cronobacter spp. (formerly Enterobacter sakazakii) are special foodborne pathogens. Cronobacter infection can cause necrotizing enterocolitis, sepsis and meningitis in all age groups, especially neonates and infants, with a high fatality of up to 80%, although the infection is rare. Outbreaks of Cronobacter infection are epidemiologically proven to be associated with contaminated powdered infant formula (PIF). Cronobacter spp. can resist dry environments and survive for a long period in food with low water activity. Therefore, Cronobacter spp. have become serious pathogens of neonates and infants, as well as in the dairy industry. In this review, we present the taxonomy, pathogenesis, resistance, detection and control of Cronobacter spp.

One intrinsic characteristic of low-moisture foods that is frequently overlooked is pH. Although pH affects the survival of microorganisms in high-moisture foods, its influence in low-moisture foods with less available moisture has not been examined. Escherichia coli O157:H7, E. coli O121, Salmonella enterica Anatum, and S. enterica Agona were grown on solid media with and without added glucose, harvested, and then suspended in buffer at pH 4, 5, and 7 for 10 min. All cultures were spotted individually onto cellulose filters and dried in a biohazard cabinet (23 ± 2°C) overnight (24 ± 2 h) and then stored in a 25°C incubator at 33% relative humidity. Populations were examined at regular intervals up to 26 (E. coli) or 29 (Salmonella) days. Additional controls for pH consisted of cultures held in buffer at pH 4, 5, and 7 at 25°C for the same time periods as the desiccated cells. For all strains tested, pH had an effect on survival whether stored dried or in liquid buffer (P < 0.05). However, when grown on solid media, acid adaptation (grown with glucose) before acid treatment did not appear beneficial to Salmonella during desiccation. Instead, both acid-adapted Salmonella serovars appeared less resistant during drying than did non–acid-adapted cells. Once dried, the rates of decline for Salmonella were not significantly different for acid-adapted and nonadapted cells (P > 0.05), indicating similar persistence following desiccation. A reverse trend was observed for E. coli O121; acid adaptation on solid media improved survival during desiccation and subsequent storage at low pH (P < 0.05). E. coli O157:H7 survival was significantly lower than that of either Salmonella or E. coli O121 under all conditions tested. Results indicate that the response to desiccation and pH stress differs between the microorganisms and under different growth conditions. [ABSTRACT FROM AUTHOR]

[Display omitted] • Essential genes envZ - ompR , recA , and flhD were identified as critical for Cronobacter sakazaki i desiccation tolerance. • Comparative proteomics highlighted maltodextrin-binding protein ESA_03421 as a key factor regulated by these genes in desiccation tolerance. • Knockout of ESA_03421 significantly reduced desiccation tolerance. • Maltose and maltodextrin enhance desiccation tolerance, molecular docking emphasizes their vital protein binding.Maltose and maltodextrin enhance desiccation tolerance， molecular docking emphasizes their vital protein binding. Cronobacter sakazakii (C. sakazakii) is a notorious pathogen responsible for infections in infants and newborns, often transmitted through contaminated infant formula. Despite the use of traditional pasteurization methods, which can reduce microbial contamination, there remains a significant risk of pathogenic C. sakazakii surviving due to its exceptional stress tolerance. In our study, we employed a comparative proteomic approach by comparing wild-type strains with gene knockout strains to identify the essential genes crucial for the successful survival of C. sakazakii during desiccation. Our investigation revealed the significance of envZ-ompR , recA , and flhD gene cassettes in contributing to desiccation tolerance in C. sakazakii. Furthermore, through our comparative proteomic profiling, we identified the maltodextrin-binding protein encoded by ESA_03421 as a potential factor influencing dry tolerance. This protein is regulated by EnvZ-OmpR , RecA , and FlhD. Notably, the knockout of ESA_03421 resulted in a 150% greater reduction in Log CFU compared to the wild-type C. sakazakii. Overall, our findings offer valuable insights into the mechanisms underlying C. sakazakii desiccation tolerance and provide potential targets for the development of new antimicrobial strategies aimed at reducing the risk of infections in infants and newborns. [ABSTRACT FROM AUTHOR]

Some Salmonella enterica serovars are frequently associated with disease outbreaks in low-moisture foods (LMF) due to their ability to adapt efficiently to desiccation stress. These serovars are often persistent during food processing. Disruption of these resistance responses was accomplished previously using the membrane-active lipopeptide, paenibacterin. This study was initiated to determine how desiccation resistance mechanisms are overcome when Salmonella Tennessee, a known resistant serovar, is treated with the membrane-active food additives carvacrol and thymol. Knowing that the minimum inhibitory concentrations (MICs) of carvacrol and thymol against Salmonella Tennessee are 200 and 100 µg/mL, the concentrations tested were 100–400 and 50–200 µg/mL, respectively. Results show that desiccation-adapted Salmonella Tennessee, prepared by air drying at 40% relative humidity and 22–25 °C for 24 h, was not inactivated when exposed for 4.0 h to less than 2xMIC of the two additives. Additionally, treatment of desiccation-adapted Salmonella Tennessee for 120 min with carvacrol and thymol at the MIC-level sensitized the cells (1.4–1.5 log CFU/mL reduction) to further desiccation stress. Treating desiccation-adapted Salmonella Tennessee with carvacrol and thymol induced leakage of intracellular potassium ions, reduced the biosynthesis of the osmoprotectant trehalose, reduced respiratory activity, decreased ATP production, and caused leakage of intracellular proteins and nucleic acids. Carvacrol, at 200–400 µg/mL, significantly downregulated the transcription of desiccation-related genes (proV, STM1494, and kdpA) as determined by the reverse-transcription quantitative PCR. The current study revealed some of the mechanisms by which carvacrol and thymol combat desiccation-resistant Salmonella Tennessee, raising the feasibility of using these additives to control desiccation-adapted S. enterica in LMF.

The highly xerotolerant bacterium classified as Exiguobacterium sp. Helios isolated from a solar panel in Spain showed a close relationship to Exiguobacterium sibiricum 255-15 isolated from Siberian permafrost. Xerotolerance has not been previously described as a characteristic of the extremely diverse Exiguobacterium genus, but both strains Helios and 255-15 showed higher xerotolerance than that described in the reference xerotolerant model strain Deinococcus radiodurans. Significant changes observed in the cell morphology after their desiccation suggests that the structure of cellular surface plays an important role in xerotolerance. Apart from its remarkable resistance to desiccation, Exiguobacterium sp. Helios strain shows several polyextremophilic characteristics that make it a promising chassis for biotechnological applications. Exiguobacterium sp. Helios cells produce nanoparticles of selenium in the presence of selenite linked to its resistance mechanism. Using the Lactobacillus plasmid pRCR12 that harbors a cherry marker, we have developed a transformation protocol for Exiguobacterium sp. Helios strain, being the first time that a bacterium of Exiguobacterium genus has been genetically modified. The comparison of Exiguobacterium sp. Helios and E. sibiricum 255-15 genomes revealed several interesting similarities and differences. Both strains contain a complete set of competence-related DNA transformation genes, suggesting that they might have natural competence, and an incomplete set of genes involved in sporulation; moreover, these strains not produce spores, suggesting that these genes might be involved in xerotolerance.

Salmonella enterica is increasingly linked to disease outbreaks associated with consumption of low-water activity (low-aw) foods. Persistence of the pathogen in these foods was attributed to its ability to implement desiccation-resistance mechanisms. Published knowledge about methods that disrupt desiccation resistance in S. enterica is lacking. We hypothesize that strong membrane-active compounds disrupt the desiccation resistance that S. enterica may acquire in low-aw foods or environments. The newly-discovered antimicrobial lipopeptide, paenibacterin, was the membrane-active agent investigated in this study. Strains of two S. enterica serovars, Tennessee and Eimsbuettel, with history of association with low-moisture foods were investigated. The viability of these strains did not decrease significantly during dehydration and subsequent storage in the dehydrated state. Considering that paenibacterin minimum inhibitory concentration (MIC) against S. enterica strains was 8 µg/mL, 4 to 16 µg/mL paenibacterin concentrations were tested. Within this range, desiccation-adapted S. Eimsbuettel was much more tolerant to the antimicrobial agent than was the desiccation-adapted S. Tennessee. Pre-treatment with 8 µg/mL paenibacterin increased inactivation of S. enterica during desiccation. The use of 16 µg/mL of paenibacterin or higher resulted in leakage of intracellular potassium ions from desiccation-adapted cells. Paenibacterin significantly decreased the biosynthesis of the intracellular osmoprotectant solute, trehalose, in a concentration-dependent manner. Treatment with 64 µg/mL paenibacterin increased the permeability of cytoplasmic membrane of desiccation-adapted cells. Transcription of desiccation-related genes; proV, STM1494, kdpA and otsB, in response to paenibacterin treatment, was investigated using reverse-transcription quantitative PCR. Transcription of some of these genes was down-regulated in a concentration-and strain-dependent manner. [ABSTRACT FROM AUTHOR]

Adult holometabolous insects may derive metabolic resources from either larval or adult feeding, but little is known of whether adult diets can compensate for deficiencies in the larval diet in terms of stress resistance. We investigated how stress resistance is affected and compensated for by diet across life stages in the marula fruit fly, Ceratitis cosyra (Walker) (Diptera: Tephritidae). Larvae were fed diets containing either 8% torula yeast, the standard diet used to rear this species, or 1% yeast (low protein content similar to known host fruit). At emergence, adults from each larval diet were tested for initial mass, water content, body composition, and desiccation and starvation resistance or they were allocated to one of two adult diet treatments: sucrose only, or sucrose and yeast hydrolysate. The same assays were then repeated after 10 days of adult feeding. Development on a low protein larval diet led to lower body mass and improved desiccation and starvation resistance in newly emerged adults, even though adults from the high protein larval diet had the highest water content. Adult feeding decreased desiccation or starvation resistance, regardless of the diet provided. Irrespective of larval diet history, newly emerged, unfed adults had significantly higher dehydration tolerance than those that were fed. Lipid reserves played a role in starvation resistance. There was no evidence for metabolic water from stored nutrients extending desiccation resistance. Our findings show the possibility of a nutrient-poor larval environment leading to correlated improvement in adult performance, at least in the short term. [ABSTRACT FROM AUTHOR]

Some Salmonella enterica strains survive well in low-water activity (low-aw) foods and cause frequent salmonellosis outbreaks in these products. Methods are needed to overcome such desiccation-resistant Salmonella and to improve the safety of low-aw foods. Building on a recent finding, we hypothesized that natural antimicrobial food additives, which are active against cytoplasmic membrane, could overcome this desiccation resistance phenomenon, and thus, sensitize the pathogen to drying and mild processing. Food additives were screened for the ability to cause leakage of intracellular potassium ions; retention of these ions is vital for protecting Salmonella against desiccation. Two antimicrobial food additives, carvacrol and thymol, caused considerable potassium leakage from the desiccation-resistant S. enterica serovars, Tennessee and Livingstone. Thus, carvacrol and thymol were investigated for their ability to sensitize the desiccation-adapted S. enterica to heat treatment. The combined use of food additives, at their minimum inhibitory concentrations, with heat treatment at 55 °C for 15 min caused 3.1 ± 0.21 to more than 5.5 log colony forming unit (CFU)/mL reduction in desiccation-adapted S. enterica, compared to 2.4 ± 0.53–3.2 ± 0.11 log CFU/mL reduction by sole heat treatment. Carvacrol was the additive that caused the greatest potassium leakage and sensitization of Salmonella to heat; hence, the application of this compound was investigated in a food model against Salmonella Typhimurium ASD200. Addition of carvacrol at 200 or 500 ppm into liquid milk followed by spray-drying reduced the strain’s population by 0.9 ± 0.02 and 1.3 ± 0.1 log CFU/g, respectively, compared to 0.6 ± 0.02 log CFU/g reduction for non-treated spray-dried milk. Additionally, freeze-drying of milk treated with high levels of carvacrol (5000 ppm) reduced the population of Salmonella Typhimurium ASD200 by more than 4.5 log CFU/g, compared to 1.1 ± 0.4 log CFU/g reduction for the freeze-dried untreated milk. These findings suggest that carvacrol can combat desiccation-resistant S. enterica, and thus, potentially improve the safety of low-aw foods.

The impact of drought on insects has become increasingly evident in the context of global climate change, but the physiological mechanisms of aphids’ responses to desiccating environments are still not well understood. We sampled the wheat aphid Sitobion avenae (Fabricius) (Hemiptera: Aphididae) from arid areas of northwestern China. Both desiccation-resistant and -nonresistant genotypes were identified, providing direct evidence of genetic divergence in desiccation resistance of S. avenae. Resistant genotypes of wingless S. avenae showed longer survival time and LT50 under the desiccation stress (i.e., 10% relative humidity) than nonresistant genotypes, and wingless individuals tended to have higher desiccation resistance than winged ones. Both absolute and relative water contents did not differ between the two kinds of genotypes. Resistant genotypes had lower water loss rates than nonresistant genotypes for both winged and wingless individuals, suggesting that modulation of water loss rates could be the primary strategy in resistance of this aphid against desiccation stress. Contents of cuticular hydrocarbons (CHC) (especially methyl-branched alkanes) showed significant increase for both resistant and nonresistant genotypes after exposure to the desiccation stress for 24 h. Under desiccation stress, survival time was positively correlated with contents of methyl-branched alkanes for resistant genotypes. Thus, the content of methyl-branched alkanes and their high plasticity could be closely linked to water loss rate and desiccation resistance in S. avenae. Our results provide insights into fundamental aspects and underlying mechanisms of desiccation resistance in aphids, and have significant implications for the evolution of aphid populations in the context of global warming.

The high surface area to volume ratio of terrestrial insects makes them highly susceptible to desiccation mainly through the cuticle. Cuticular permeability (CP) is usually the most important factor limiting water loss in terrestrial insects. Water loss rate, percentage of total body water (%TBW) content, CP, and desiccation tolerance were investigated in workers of four Solenopsis species in the southeastern USA. We hypothesized that tropical/subtropical ants (S. invicta and S. geminata) will have lower CP values and tolerate higher levels of desiccation than temperate ants (S. richteri and S. invicta × S. richteri). The %TBW content was similar among species. Solenopsis invicta had a 1.3-fold and 1.1-fold lower CP value than S. invicta × S. richteri and S. richteri, respectively. Solenopsis geminata had a 1.3-fold lower CP value than S. invicta × S. richteri, and a 1.2-fold lower CP value than S. richteri. The LT50 values (lethal time to kill 50% of the population) ranged from 1.5 h (small S. geminata) to 8.5 h (large S. invicta). Desiccation tolerance ranged between 36 and 50 %TBW lost at death and was not related to a species’ location of origin. This study is the first report of water relations of S. invicta × S. richteri. It demonstrates that desiccation stress differentially can affect the survival of different Solenopsis species and implies that environmental stress can affect the distribution of these species in the southeastern USA.