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Tropical mountainous regions are often identified as landslide hotspots with growing population pressure. Anthropogenic factors are assumed to play a role in the occurrence of landslides in these densely populated regions, yet the relative importance of these human-induced factors remains poorly documented. In this work, we aim to explore the impact of forest cover dynamics, roads and mining activities on the characteristics and causes of landslides in the rift flank west of Lake Kivu in the Democratic Republic of the Congo (DR Congo). To do so, we compile a comprehensive multi-temporal inventory of 2730 landslides. The landslides are of different types and are grouped into five categories that are adapted to study the impact of human activities on slope stability: old (pre-1950s) and recent (post-1950s) deep-seated landslides, shallow landslides, landslides associated with mining and landslides associated with road construction. We analyse the landslides according to this classification protocol via frequency–area statistics, frequency ratio distribution and logistic regression susceptibility assessment. We find that natural factors contributing to the cause of recent and old deep-seated landslides were either different or changed over time. Under similar topographic conditions, shallow landslides are more frequent, but of a smaller size, in areas where deforestation has occurred since the 1950s. We attribute this size reduction to the decrease in regolith cohesion due to forest loss, which allows for a smaller minimum critical area for landsliding. In areas that were already deforested in the 1950s, shallow landslides are less frequent, larger and occur on less steep slopes. This suggests a combined role between regolith availability and soil management practices that influence erosion and water infiltration. Mining activities increase the odds of landsliding. Landslides associated with mining and roads are larger than shallow landslides but smaller than the recent deep-seated instabilities, and they are controlled by environmental factors that are not present under natural conditions. Our analysis demonstrates the role of human activities on the occurrence of landslides in the Lake Kivu region. Overall, it highlights the need to consider this context when studying hillslope instability characteristics and distribution patterns in regions under anthropogenic pressure. Our work also highlights the importance of using landslide classification criteria adapted to the context of the Anthropocene.

Deforestation is associated with a decrease in slope stability through the alteration of hydrological and geotechnical conditions. As such, deforestation increases landslide activity over short, decadal timescales. However, over longer timescales (0.1–10 Myr) the location and timing of landsliding is controlled by the interaction between uplift and fluvial incision. Yet, the interaction between (human-induced) deforestation and landscape evolution has hitherto not been explicitly considered. We address this issue in the North Tanganyika–Kivu rift region (East African Rift). In recent decades, the regional population has grown exponentially, and the associated expansion of cultivated and urban land has resulted in widespread deforestation. In the past 11 Myr, active continental rifting and tectonic processes have forged two parallel mountainous rift shoulders that are continuously rejuvenated (i.e., actively incised) through knickpoint retreat, enforcing topographic steepening. In order to link deforestation and rejuvenation to landslide erosion, we compiled an inventory of nearly 8000 recent shallow landslides in © Google Earth imagery from 2000–2019. To accurately calculate landslide erosion rates, we developed a new methodology to remediate inventory biases linked to the spatial and temporal inconsistency of this satellite imagery. Moreover, to account for the impact of rock strength on both landslide occurrence and knickpoint retreat, we limit our analysis to rock types with threshold angles of 24–28∘. Rejuvenated landscapes were defined as the areas draining towards Lake Kivu or Lake Tanganyika and downstream of retreating knickpoints. We find that shallow landslide erosion rates in these rejuvenated landscapes are roughly 40 % higher than in the surrounding relict landscapes. In contrast, we find that slope exerts a stronger control on landslide erosion in relict landscapes. These two results are reconciled by the observation that landslide erosion generally increases with slope gradient and that the relief is on average steeper in rejuvenated landscapes. The weaker effect of slope steepness on landslide erosion rates in the rejuvenated landscapes could be the result of three factors: the absence of earthquake-induced landslide events in our landslide inventory, a thinner regolith mantle, and a drier climate. More frequent extreme rainfall events in the relict landscapes, and the presence of a thicker regolith, may explain a stronger landslide response to deforestation compared to rejuvenated landscapes. Overall, deforestation initiates a landslide peak that lasts approximately 15 years and increases landslide erosion by a factor 2 to 8. Eventually, landslide erosion in deforested land falls back to a level similar to that observed under forest conditions, most likely due to the depletion of the most unstable regolith. Landslides are not only more abundant in rejuvenated landscapes but are also smaller in size, which may again be a consequence of a thinner regolith mantle and/or seismic activity that fractures the bedrock and reduces the minimal critical area for slope failure. With this paper, we highlight the importance of considering the geomorphological context when studying the impact of recent land use changes on landslide activity.

In recent decades, large wildfires have inflicted considerable damage on valuable Natura 2000 regions in Belgium. Despite these events and the general perception that global change will exacerbate wildfire prevalence, this has not been studied yet in the Belgian context. Therefore, the national government initiated the national action plan on wildfires in order to evaluate the wildfire risk, on the one hand, and the materials, procedures, and training of fire services, on the other hand. This study focuses on the spatial distribution of the ignition probability, a component of the wildfire risk framework. In a first stage, we compile a historical wildfire database using (i) newspaper articles between 1994 and 2016 and (ii) a list of wildfire interventions between 2010 and 2013, provided by the government. In a second stage, we use a straightforward method relying on Bayes' rule and a limited number of covariates to calculate the ignition probability. It appears that most wildfire-prone areas in Belgium are located in heathland where military exercises are held. The provinces that have the largest relative areas with a high or very high wildfire risk are Limburg and Antwerp. Our study also revealed that most wildfire ignitions in Belgium are caused by humans (both arson and negligence) and that natural causes such as lightning are rather scarce. Wildfire prevention can be improved by (i) excluding military activity in fire-prone areas during the fire season, (ii) improving collaboration with foreign emergency services, (iii) concentrating the dedicated resources in the areas that display the highest ignition probabilities, (iv) improving fire detection methods, and (v) raising more awareness among the public.

Although many recombinant proteins have been produced in seeds at high yields without adverse effects on the plant, endoplasmic reticulum (ER) stress and aberrant localization of endogenous or recombinant proteins have also been reported. The production of murine interleukin-10 (mIL-10) in Arabidopsis thaliana seeds resulted in the de novo formation of ER-derived structures containing a large fraction of the recombinant protein in an insoluble form. These bodies containing mIL-10 were morphologically similar to Russell bodies found in mammalian cells. We confirmed that the compartment containing mIL-10 was enclosed by ER membranes, and 3D electron microscopy revealed that these structures have a spheroidal shape. Another feature shared with Russell bodies is the continued viability of the cells that generate these organelles. To investigate similarities in the formation of Russell-like bodies and the plant-specific protein bodies formed by prolamins in cereal seeds, we crossed plants containing ectopic ER-derived prolamin protein bodies with a line accumulating mIL-10 in Russell-like bodies. This resulted in seeds containing only one population of protein bodies in which mIL-10 inclusions formed a central core surrounded by the prolamin-containing matrix, suggesting that both types of protein aggregates are together removed from the secretory pathway by a common mechanism. We propose that, like mammalian cells, plant cells are able to form Russell-like bodies as a self-protection mechanism, when they are overloaded with a partially transport-incompetent protein, and we discuss the resulting challenges for recombinant protein production.

Accurate and detailed multitemporal inventories of landslides and their process characterization are crucial for the evaluation of landslide hazards and the implementation of disaster risk reduction strategies in densely-populated mountainous regions. Such investigations are, however, rare in many regions of the tropical African highlands, where landslide research is often in its infancy and not adapted to the local needs. Here, we have produced a comprehensive multitemporal investigation of the landslide processes in the hillslopes of Bujumbura, situated in the landslide-prone East African Rift. We inventoried more than 1200 landslides by combining careful field investigation and visual analysis of satellite images, very-high-resolution topographic data, and historical aerial photographs. More than 20% of the hillslopes of the city are affected by landslides. Recent landslides (post-1950s) are mostly shallow, triggered by rainfall, and located on the steepest slopes. The presence of roads and river quarrying can also control their occurrence. Deep-seated landslides typically concentrate in landscapes that have been rejuvenated through knickpoint retreat. The difference in size distributions between old and recent deep-seated landslides suggests the long-term influence of potentially changing slope-failure drivers. Of the deep-seated landslides, 66% are currently active, those being mostly earthflows connected to the river system. Gully systems causing landslides are commonly associated with the urbanization of the hillslopes. Our results provide a much more accurate record of landslide processes and their impacts in the region than was previously available. These insights will be useful for land management and disaster risk reduction strategies.

Campylobacteriosis is a widespread infectious disease, leading to a major health and economic burden. Chickens are considered as the most common infection source for humans. Campylobacter mainly multiplies in the mucus layer of their caeca. No effective control measures are currently available, but passive immunisation of chickens with pathogen-specific maternal IgY antibodies, present in egg yolk of immunised chickens, reduces Campylobacter colonisation. To explore this strategy further, anti-Campylobacter nanobodies, directed against the flagella and major outer membrane proteins, were fused to the constant domains of chicken IgA and IgY, combining the benefits of nanobodies and the effector functions of the Fc-domains. The designer chimeric antibodies were effectively produced in leaves of Nicotiana benthamiana and seeds of Arabidopsis thaliana. Stable expression of the chimeric antibodies in seeds resulted in production levels between 1% and 8% of the total soluble protein. These in planta produced antibodies do not only bind to their purified antigens but also to Campylobacter bacterial cells. In addition, the anti-flagellin chimeric antibodies are reducing the motility of Campylobacter bacteria. These antibody-containing Arabidopsis seeds can be tested for oral passive immunisation of chickens and, if effective, the chimeric antibodies can be produced in crop seeds.

To evaluate the chromosomal background of different Agrobacterium strains on floral dip transformation frequency, eight wild-type Agrobacterium strains, provided by Laboratorium voor Microbiologie Gent (LMG) and classified in different genomic groups, were compared with the commonly used Agrobacterium strains C58C1 Rifr (pMP90) and LBA4404 in Arabidopsis thaliana Columbia (Col-0) and C24 ecotypes. The C58C1 Rifr chromosomal background in combination with the pMP90 virulence plasmid showed high Col-0 floral dip transformation frequencies (0.76 to 1.57%). LMG201, which is genetically close to the Agrobacterium C58 strain, with the same virulence plasmid showed comparable or even higher transformation frequencies (1.22 to 2.28%), whereas the LBA4404 strain displayed reproducibly lower transformation frequencies (