1. Sequence and polymorphism analysis of the camel (Camelus dromedarius) myostatin gene
- Author
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Elena Ciani, Pamela A. Burger, Youcef Amine Cherifi, Stefania Muzzachi, Habib Yahyaoui, Bernard Faye, Giovanni Michele Lacalandra, Ahmad Oulmouden, Mohamed Ali Zayed, Department of Biosciences, Biotechnology and Biopharmaceutics, Università degli studi di Bari, Unité de Génétique Moléculaire Animale (UGMA), Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA), Département de Génétique Moléculaire Appliquée, Faculté des Sciences de la Nature, Laboratoire de Génétique Moléculaire et Cellulaire, Université des sciences et de la Technologie d'Oran Mohamed Boudiaf [Oran] (USTO MB), Laboratoire d'élevage et de la Faune Sauvage, Institut des Régions Arides de Médenine (IRA), Department of Animal Breeding, Meat Quality and Safety, Desert Research Centre (DRC), Institute of Population Genetics, University of Veterinary Medicine [Vienna] (Vetmeduni), Department of Emergency and Organ Transplantation, Section of Animal Production, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), MOA Camel Project, Food and Agriculture Organization, European Union within the ENPI-CBC-MED I.B/1.1/493, Università degli studi di Bari Aldo Moro (UNIBA), Unité de Génétique Moléculaire Animale (UMR GMA), Institut National de la Recherche Agronomique (INRA)-Université de Limoges (UNILIM), and Institut des Régions Arides (IRA)
- Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences ,Polymorphisme génétique ,Évolution ,Myostatin ,polymorphisme ,Applied Microbiology and Biotechnology ,polymorphism ,Séquence d'ADN ,Facteur de croissance ,camelus dromedarius ,myostatin ,sequence ,L52 - Physiologie animale - Croissance et développement ,Genetics ,education.field_of_study ,biology ,Nucleic acid sequence ,muscle squelettique ,Agricultural sciences ,variabilité de séquence ,Alimentation et Nutrition ,Camelus ,Population ,Dromadaire ,Locus (genetics) ,Variation génétique ,myostatine ,Food and Nutrition ,education ,Gene ,Génie génétique ,Genetic diversity ,Intron ,Tylopoda ,biology.organism_classification ,L10 - Génétique et amélioration des animaux ,biology.protein ,clonage ,Animal Science and Zoology ,Agronomy and Crop Science ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Sciences agricoles ,Food Science - Abstract
Myostatin (MSTN), a negative regulator of skeletal muscle development in mammals, represents a key target for genetic investigations in meat-producing animals, with mutations responsible for increased skeletal-muscle mass currently described in several livestock species. Dromedary camels play a major economic role as suppliers of meat for human consumption across several countries. Notwithstanding, a comprehensive characterization of the sequence variability at the Camelus dromedarius MSTN locus was still lacking. Here we present the first extensive sequence and polymorphism analysis of the MSTN gene in the Camelus dromedarius species. Out of more than 3.6 kb of nucleotide sequence screened on 22 animals from 3 different Northern African regions, only 3 variant sites in the first intron were detected. The low observed diversity may reflect the evolutionary history of the species, likely developed as domesticates from a low variable wild ancestor population. Sequence identity among Camelus dromedarius and other Cetartiodactyla highlighted a tree topology consistent with previous reports of a closer relationship between Tylopoda and Suiformes. A close similarity between C. ferus and Camelus dromedarius was observed within Tylopoda. A markedly higher sequence identity between Camelus dromedarius and the other vertebrate species was observed at the MSTN locus compared to other genes, thus confirming it as a highly conserved target across mammals.
- Published
- 2015
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