69 results on '"Dentener MA"'
Search Results
2. Presence of bactericidal/permeability-increasing protein in disease: detection by ELISA.
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Dentener MA, Francot GJ, Smit FT, Froon AH, Pennings HJ, Wouters EF, Buurman WA, Dentener, M A, Francot, G J, Smit, F T, Froon, A H, Pennings, H J, Wouters, E F, and Buurman, W A
- Abstract
A sandwich ELISA was developed specific for human bactericidal/permeability-increasing protein (BPI), using Mg++ ions to abrogate disturbance by lipopolysaccharide of BPI measurement and to prevent aspecific adherence of BPI to solid phase. In fresh EDTA or heparinized plasma of healthy volunteers BPI was not detectable, whereas in serum BPI was present, indicating that coagulation activates polymorphonuclear leukocytes to release BPI. Furthermore, BPI was present in plasma of critically ill intensive care unit (ICU) patients, in bronchoalveolar lavage fluid of patients suspected of having pneumonia, in wound fluid, and in pleural fluid. In sub-groups of samples with culture-proven bacteria, mean BPI levels were increased compared with subgroups without bacteria, although the differences were only significant in EDTA plasma of ICU patients. These findings indicate the presence of BPI during pathologic conditions. The physiologic role of the released BPI has to be further elucidated. [ABSTRACT FROM AUTHOR]
- Published
- 1995
3. Tumor necrosis factor inhibitors for rheumatoid arthritis.
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Roos JC, Ostor AJ, Okamoto H, Dentener MA, Wouters EFM, Scott DL, and Kingsley GH
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- 2006
4. Smoking-Associated Exposure of Human Primary Bronchial Epithelial Cells to Aldehydes: Impact on Molecular Mechanisms Controlling Mitochondrial Content and Function.
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Tulen CBM, Duistermaat E, Cremers JWJM, Klerx WNM, Fokkens PHB, Weibolt N, Kloosterboer N, Dentener MA, Gremmer ER, Jessen PJJ, Koene EJC, Maas L, Opperhuizen A, van Schooten FJ, Staal YCM, and Remels AHV
- Subjects
- Humans, Acrolein toxicity, Acrolein metabolism, Epithelial Cells metabolism, Mitochondria metabolism, Acetaldehyde toxicity, Acetaldehyde metabolism, Nicotiana, Formaldehyde, RNA, Messenger metabolism, Smoking, Aldehydes metabolism, Pulmonary Disease, Chronic Obstructive metabolism
- Abstract
Chronic obstructive pulmonary disease (COPD) is a devastating lung disease primarily caused by exposure to cigarette smoke (CS). During the pyrolysis and combustion of tobacco, reactive aldehydes such as acetaldehyde, acrolein, and formaldehyde are formed, which are known to be involved in respiratory toxicity. Although CS-induced mitochondrial dysfunction has been implicated in the pathophysiology of COPD, the role of aldehydes therein is incompletely understood. To investigate this, we used a physiologically relevant in vitro exposure model of differentiated human primary bronchial epithelial cells (PBEC) exposed to CS (one cigarette) or a mixture of acetaldehyde, acrolein, and formaldehyde (at relevant concentrations of one cigarette) or air, in a continuous flow system using a puff-like exposure protocol. Exposure of PBEC to CS resulted in elevated IL-8 cytokine and mRNA levels, increased abundance of constituents associated with autophagy, decreased protein levels of molecules associated with the mitophagy machinery, and alterations in the abundance of regulators of mitochondrial biogenesis. Furthermore, decreased transcript levels of basal epithelial cell marker KRT5 were reported after CS exposure. Only parts of these changes were replicated in PBEC upon exposure to a combination of acetaldehyde, acrolein, and formaldehyde. More specifically, aldehydes decreased MAP1LC3A mRNA (autophagy) and BNIP3 protein (mitophagy) and increased ESRRA protein (mitochondrial biogenesis). These data suggest that other compounds in addition to aldehydes in CS contribute to CS-induced dysregulation of constituents controlling mitochondrial content and function in airway epithelial cells.
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- 2022
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5. Crystalline silica alters Sulfatase-1 expression in rat lungs which influences hyper-proliferative and fibrogenic effects in human lung epithelial cells.
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Perkins TN, Peeters PM, Albrecht C, Schins RPF, Dentener MA, Mossman BT, Wouters EFM, and Reynaert NL
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- Animals, Cell Line, Collagen metabolism, Crystallization, Down-Regulation, Epithelial Cells enzymology, Epithelial Cells pathology, Female, Heparin analogs & derivatives, Heparin metabolism, Humans, Lung enzymology, Lung pathology, Proteoglycans metabolism, Pulmonary Fibrosis enzymology, Pulmonary Fibrosis genetics, Pulmonary Fibrosis pathology, Rats, Wistar, Signal Transduction drug effects, Silicon Dioxide chemistry, Silicosis enzymology, Silicosis genetics, Silicosis pathology, Sulfotransferases genetics, Time Factors, Cell Proliferation drug effects, Epithelial Cells drug effects, Lung drug effects, Pulmonary Fibrosis chemically induced, Silicon Dioxide toxicity, Silicosis etiology, Sulfotransferases metabolism
- Abstract
Lung epithelial cells are the first cell-type to come in contact with hazardous dust materials. Upon deposition, they invoke complex reactions in attempt to eradicate particles from the airways, and repair damage. The cell surface is composed of a heterogeneous network of matrix proteins and proteoglycans, which act as scaffold and control cell-signaling networks. These functions are controlled, in part, by the sulfation patterns of heparin-sulfate proteoglycans (HSPGs), which are enzymatically regulated. Although there is evidence of altered HSPG-sulfation in idiopathic pulmonary fibrosis (IPF), this is not investigated in silicosis. Our previous studies revealed down-regulation of Sulfatase-1 (SULF1) in human bronchial epithelial cells (BECs) by crystalline silica (CS). In this study, CS-induced down-regulation of SULF1, and increases in Sulfated-HSPGs, were determined in human BECs, and in rat lungs. By siRNA and plasmid transfection techniques the effects of SULF1 expression on silica-induced fibrogenic and proliferative gene expression were determined. These studies confirmed down-regulation of SULF1 and subsequent increases in sulfated-HSPGs in vitro. Moreover, short-term exposure of rats to CS resulted in similar changes in vivo. Conversely, effects were reversed after long term CS exposure of rats. SULF1 knockdown, and overexpression alleviated and exacerbated silica-induced decrease in cell viability, respectively. Furthermore, overexpression of SULF1 promoted silica-induced proliferative and fibrogenic gene expression, and collagen production. These findings demonstrate that the HSPG modification enzyme SULF1 and HSPG sulfation are altered by CS in vitro and in vivo. Furthermore, these changes may contribute to CS-induced lung pathogenicity by affecting injury tolerance, hyperproliferation, and fibrotic effects., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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6. Involvement of c-Jun N-Terminal Kinase in TNF-α-Driven Remodeling.
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Eurlings IM, Reynaert NL, van de Wetering C, Aesif SW, Mercken EM, de Cabo R, van der Velden JL, Janssen-Heininger YM, Wouters EF, and Dentener MA
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- Animals, Biomarkers metabolism, Cell Hypoxia drug effects, Cell Line, Epithelial Cells drug effects, Epithelial Cells metabolism, Extracellular Matrix drug effects, Humans, JNK Mitogen-Activated Protein Kinases antagonists & inhibitors, Lung drug effects, Lung metabolism, Mesoderm metabolism, Mice, Phenotype, Phosphorylation drug effects, Pulmonary Disease, Chronic Obstructive metabolism, Pulmonary Disease, Chronic Obstructive pathology, Pulmonary Surfactant-Associated Protein C metabolism, Signal Transduction drug effects, Extracellular Matrix metabolism, JNK Mitogen-Activated Protein Kinases metabolism, Tumor Necrosis Factor-alpha pharmacology
- Abstract
Lung tissue remodeling in chronic obstructive pulmonary disease (COPD) is characterized by airway wall thickening and/or emphysema. Although the bronchial and alveolar compartments are functionally independent entities, we recently showed comparable alterations in matrix composition comprised of decreased elastin content and increased collagen and hyaluronan contents of alveolar and small airway walls. Out of several animal models tested, surfactant protein C (SPC)-TNF-α mice showed remodeling in alveolar and airway walls similar to what we observed in patients with COPD. Epithelial cells are able to undergo a phenotypic shift, gaining mesenchymal properties, a process in which c-Jun N-terminal kinase (JNK) signaling is involved. Therefore, we hypothesized that TNF-α induces JNK-dependent epithelial plasticity, which contributes to lung matrix remodeling. To this end, the ability of TNF-α to induce a phenotypic shift was assessed in A549, BEAS2B, and primary bronchial epithelial cells, and phenotypic markers were studied in SPC-TNF-α mice. Phenotypic markers of mesenchymal cells were elevated both in vitro and in vivo, as shown by the expression of vimentin, plasminogen activator inhibitor-1, collagen, and matrix metalloproteinases. Concurrently, the expression of the epithelial markers, E-cadherin and keratin 7 and 18, was attenuated. A pharmacological inhibitor of JNK attenuated this phenotypic shift in vitro, demonstrating involvement of JNK signaling in this process. Interestingly, activation of JNK signaling was also clearly present in lungs of SPC-TNF-α mice and patients with COPD. Together, these data show a role for TNF-α in the induction of a phenotypic shift in vitro, resulting in increased collagen production and the expression of elastin-degrading matrix metalloproteinases, and provide evidence for involvement of the TNF-α-JNK axis in extracellular matrix remodeling.
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- 2017
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7. Alteration of canonical and non-canonical WNT-signaling by crystalline silica in human lung epithelial cells.
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Perkins TN, Dentener MA, Stassen FR, Rohde GG, Mossman BT, Wouters EF, and Reynaert NL
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- Cell Line, Cells, Cultured, Epithelial Cells metabolism, Humans, Lung cytology, Oligonucleotide Array Sequence Analysis, Transcriptome, Epithelial Cells drug effects, Silicon Dioxide toxicity, Wnt Signaling Pathway drug effects
- Abstract
Growth and development of the mature lung is a complex process orchestrated by a number of intricate developmental signaling pathways. Wingless-type MMTV-integration site (WNT) signaling plays critical roles in controlling branching morphogenesis cell differentiation, and formation of the conducting and respiratory airways. In addition, WNT pathways are often re-activated in mature lungs during repair and regeneration. WNT- signaling has been elucidated as a crucial contributor to the development of idiopathic pulmonary fibrosis as well as other hyper-proliferative lung diseases. Silicosis, a detrimental occupational lung disease caused by excessive inhalation of crystalline silica dust, is hallmarked by repeated cycles of damaging inflammation, epithelial hyperplasia, and formation of dense, hyalinized nodules of whorled collagen. However, mechanisms of epithelial cell hyperplasia and matrix deposition are not well understood, as most research efforts have focused on the pronounced inflammatory response. Microarray data from our previous studies has revealed a number of WNT-signaling and WNT-target genes altered by crystalline silica in human lung epithelial cells. In the present study, we utilize pathway analysis to designate connections between genes altered by silica in WNT-signaling networks. Furthermore, we confirm microarray findings by QRT-PCR and demonstrate both activation of canonical (β-catenin) and down-regulation of non-canonical (WNT5A) signaling in immortalized (BEAS-2B) and primary (PBEC) human bronchial epithelial cells. These findings suggest that WNT-signaling and cross-talk with other pathways (e.g. Notch), may contribute to proliferative, fibrogenic and inflammatory responses to silica in lung epithelial cells., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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8. Exposure to common respiratory bacteria alters the airway epithelial response to subsequent viral infection.
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Bellinghausen C, Gulraiz F, Heinzmann AC, Dentener MA, Savelkoul PH, Wouters EF, Rohde GG, and Stassen FR
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- Adenoviridae pathogenicity, Animals, Bacteria immunology, Chlorocebus aethiops, Cytokines metabolism, Dogs, Epithelial Cells metabolism, Haemophilus influenzae pathogenicity, HeLa Cells, Host-Pathogen Interactions, Humans, Inflammation Mediators metabolism, Influenza B virus pathogenicity, Lung metabolism, Madin Darby Canine Kidney Cells, Primary Cell Culture, Pseudomonas aeruginosa pathogenicity, Respiratory Syncytial Viruses pathogenicity, Respiratory Tract Infections metabolism, Streptococcus pneumoniae pathogenicity, Time Factors, Vero Cells, Viruses immunology, Bacteria pathogenicity, Coinfection, Epithelial Cells microbiology, Epithelial Cells virology, Lung microbiology, Lung virology, Respiratory Tract Infections microbiology, Respiratory Tract Infections virology, Viruses pathogenicity
- Abstract
Background: Colonization of the airways with potential pathogenic bacteria is observed in a number of chronic respiratory diseases, such as COPD or cystic fibrosis. Infections with respiratory viruses are known triggers of exacerbations of these diseases. We here investigated if pre-exposure to bacteria alters the response of lung epithelial cells to subsequent viral infection., Methods: Bronchial epithelial cells (BEAS-2B cells and primary bronchial epithelial cells) were exposed to heat-inactivated Haemophilus influenzae, Pseudomonas aeruginosa or Streptococcus pneumoniae and subsequently infected with respiratory syncytial virus (RSV), type 2 human adenovirus or influenza B. Levels of pro-inflammatory cytokines, viral replication and expression of pattern recognition receptors were determined in culture supernatants and/or cell lysates., Results: Exposure of BEAS-2B cells to H. influenzae before and during RSV-infection synergistically increased the release of IL-6 (increase above calculated additive effect at 72 h: 56 % ± 3 %, mean ± SEM) and IL-8 (53 % ± 12 %). This effect was sustained even when bacteria were washed away before viral infection and was neither associated with enhanced viral replication, nor linked to increased expression of key pattern recognition receptors. P. aeruginosa enhanced the release of inflammatory cytokines to a similar extent, yet only if bacteria were also present during viral infection. S. pneumoniae did not enhance RSV-induced cytokine release. Surprisingly, adenovirus infection significantly reduced IL-6 release in cells exposed to either of the three tested bacterial strains by on average more than 50 %. Infection with influenza B on the other hand did not affect cytokine production in BEAS-2B cells exposed to the different bacterial strains., Conclusion: Pre-exposure of epithelial cells to bacteria alters the response to subsequent viral infection depending on the types of pathogen involved. These findings highlight the complexity of microbiome interactions in the airways, possibly contributing to the susceptibility to exacerbations and the natural course of airway diseases.
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- 2016
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9. Cigarette smoke extract induces a phenotypic shift in epithelial cells; involvement of HIF1α in mesenchymal transition.
- Author
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Eurlings IM, Reynaert NL, van den Beucken T, Gosker HR, de Theije CC, Verhamme FM, Bracke KR, Wouters EF, and Dentener MA
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- Animals, Biomarkers metabolism, Bronchi cytology, Cell Hypoxia drug effects, Cell Line, Humans, Mice, Phenotype, Pulmonary Alveoli cytology, Signal Transduction drug effects, Transforming Growth Factor beta metabolism, Epithelial Cells cytology, Epithelial Cells drug effects, Epithelial-Mesenchymal Transition drug effects, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Smoke adverse effects, Nicotiana chemistry
- Abstract
In COPD, matrix remodeling contributes to airflow limitation. Recent evidence suggests that next to fibroblasts, the process of epithelial-mesenchymal transition can contribute to matrix remodeling. CSE has been shown to induce EMT in lung epithelial cells, but the signaling mechanisms involved are largely unknown and subject of this study. EMT was assessed in A549 and BEAS2B cells stimulated with CSE by qPCR, Western blotting and immunofluorescence for epithelial and mesenchymal markers, as were collagen production, cell adhesion and barrier integrity as functional endpoints. Involvement of TGF-β and HIF1α signaling pathways were investigated. In addition, mouse models were used to examine the effects of CS on hypoxia signaling and of hypoxia per se on mesenchymal expression. CSE induced EMT characteristics in A549 and BEAS2B cells, evidenced by decreased expression of epithelial markers and a concomitant increase in mesenchymal marker expression after CSE exposure. Furthermore cells that underwent EMT showed increased production of collagen, decreased adhesion and disrupted barrier integrity. The induction of EMT was found to be independent of TGF-β signaling. On the contrary, CS was able to induce hypoxic signaling in A549 and BEAS2B cells as well as in mice lung tissue. Importantly, HIF1α knock-down prevented induction of mesenchymal markers, increased collagen production and decreased adhesion after CSE exposure, data that are in line with the observed induction of mesenchymal marker expression by hypoxia in vitro and in vivo. Together these data provide evidence that both bronchial and alveolar epithelial cells undergo a functional phenotypic shift in response to CSE exposure which can contribute to increased collagen deposition in COPD lungs. Moreover, HIF1α signaling appears to play an important role in this process.
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- 2014
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10. A comparative study of matrix remodeling in chronic models for COPD; mechanistic insights into the role of TNF-α.
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Eurlings IM, Dentener MA, Mercken EM, de Cabo R, Bracke KR, Vernooy JH, Wouters EF, and Reynaert NL
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- Airway Remodeling immunology, Animals, Disease Models, Animal, Elastin metabolism, Extracellular Matrix pathology, Fibrillar Collagens metabolism, Gene Expression, Hyaluronic Acid metabolism, Lipopolysaccharides pharmacology, Male, Matrix Metalloproteinase 2 genetics, Matrix Metalloproteinase 2 metabolism, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Mice, Mice, Inbred C57BL, Pulmonary Alveoli immunology, Pulmonary Alveoli metabolism, Pulmonary Alveoli pathology, Pulmonary Disease, Chronic Obstructive etiology, Pulmonary Disease, Chronic Obstructive immunology, Smoking adverse effects, Extracellular Matrix metabolism, Pulmonary Disease, Chronic Obstructive metabolism, Tumor Necrosis Factor-alpha physiology
- Abstract
Remodeling in chronic obstructive pulmonary disease (COPD) has at least two dimensions: small airway wall thickening and destruction of alveolar walls. Recently we showed comparable alterations of the extracellular matrix (ECM) compounds collagen, hyaluoran, and elastin in alveolar and small airway walls of COPD patients. The aim of this study was to characterize and assess similarities in alveolar and small airway wall matrix remodeling in chronic COPD models. From this comparative characterization of matrix remodeling we derived and elaborated underlying mechanisms to the matrix changes reported in COPD. Lung tissue sections of chronic models for COPD, either induced by exposure to cigarette smoke, chronic intratracheal lipopolysaccharide instillation, or local tumor necrosis factor (TNF) expression [surfactant protein C (SPC)-TNFα mice], were stained for elastin, collagen, and hyaluronan. Furthermore TNF-α matrix metalloproteinase (MMP)-2, -9, and -12 mRNA expression was analyzed using qPCR and localized using immunohistochemistry. Both collagen and hyaluronan were increased in alveolar and small airway walls of all three models. Interestingly, elastin contents were differentially affected, with a decrease in both alveolar and airway walls in SPC-TNFα mice. Furthermore TNF-α and MMP-2 and -9 mRNA and protein levels were found to be increased in alveolar walls and around airway walls only in SPC-TNFα mice. We show that only SPC-TNFα mice show changes in elastin remodeling that are comparable to what has been observed in COPD patients. This reveals that the SPC-TNFα model is a suitable model to study processes underlying matrix remodeling and in particular elastin breakdown as seen in COPD. Furthermore we indicate a possible role for MMP-2 and MMP-9 in the breakdown of elastin in airways and alveoli of SPC-TNFα mice., (Copyright © 2014 the American Physiological Society.)
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- 2014
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11. Similar matrix alterations in alveolar and small airway walls of COPD patients.
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Eurlings IM, Dentener MA, Cleutjens JP, Peutz CJ, Rohde GG, Wouters EF, and Reynaert NL
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- Biopsy, Needle, Case-Control Studies, Chi-Square Distribution, Elastin metabolism, Female, Fibrillar Collagens metabolism, Humans, Immunohistochemistry, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive physiopathology, Reference Values, Severity of Illness Index, Smad2 Protein metabolism, Airway Remodeling, Bronchi pathology, Extracellular Matrix metabolism, Pulmonary Alveoli pathology, Pulmonary Disease, Chronic Obstructive pathology
- Abstract
Background: Remodelling in COPD has at least two dimensions: small airway wall thickening and destruction of alveolar walls. Recent studies indicate that there is some similarity between alveolar and small airway wall matrix remodelling. The aim of this study was to characterise and assess similarities in alveolar and small airway wall matrix remodelling, and TGF-β signalling in COPD patients of different GOLD stages., Methods: Lung tissue sections of 14 smoking controls, 16 GOLD II and 19 GOLD IV patients were included and stained for elastin and collagens as well as hyaluronan, a glycosaminoglycan matrix component and pSMAD2., Results: Elastin was significantly decreased in COPD patients not only in alveolar, but also in small airway walls. Interestingly, both collagen and hyaluronan were increased in alveolar as well as small airway walls. The matrix changes were highly comparable between GOLD stages, with collagen content in the alveolar wall increasing further in GOLD IV. A calculated remodelling index, defined as elastin divided over collagen and hyaluronan, was decreased significantly in GOLD II and further lowered in GOLD IV patients, suggesting that matrix component alterations are involved in progressive airflow limitation. Interestingly, there was a positive correlation present between the alveolar and small airway wall stainings of the matrix components, as well as for pSMAD2. No differences in pSMAD2 staining between controls and COPD patients were found., Conclusions: In conclusion, remodelling in the alveolar and small airway wall in COPD is markedly similar and already present in moderate COPD. Notably, alveolar collagen and a remodelling index relate to lung function.
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- 2014
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12. Efficacy of IFN-λ1 to protect human airway epithelial cells against human rhinovirus 1B infection.
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Gulraiz F, Bellinghausen C, Dentener MA, Reynaert NL, Gaajetaan GR, Beuken EV, Rohde GG, Bruggeman CA, and Stassen FR
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- Antiviral Agents pharmacology, Antiviral Agents therapeutic use, Cell Line, Cytoprotection drug effects, Epithelial Cells drug effects, Humans, Interferon-beta metabolism, Interferons, Interleukins pharmacology, Picornaviridae Infections virology, Recombinant Proteins pharmacology, Recombinant Proteins therapeutic use, Rhinovirus drug effects, Time Factors, Bronchi pathology, Epithelial Cells pathology, Epithelial Cells virology, Interleukins therapeutic use, Picornaviridae Infections drug therapy, Picornaviridae Infections prevention & control, Rhinovirus physiology
- Abstract
Impaired interferon (IFN) production has been observed in various obstructive respiratory diseases. This contributes to enhanced sensitivity towards viral infections triggering acute exacerbations. To compensate for this impaired host IFN response, there is need to explore new therapeutic strategies, like exogenous administration of IFNs as prophylactic treatment. In the present study, we examined the protective potential of IFN-λ1 and compared it with the previously established protecting effect of IFN-β. A549 cells and human primary bronchial epithelial cells were first treated with either IFN-β (500 IU/ml) or IFN-λ1 (500 ng/ml) for 18 h. For infection, two approaches were adopted: i) Continuous scenario: after pre-treatment, cells were infected immediately for 24 h with human rhinovirus 1B (HRV1B) in IFN-containing medium, or were cultured for another 72 h in IFN-containing medium, and then infected for 24 h with HRV1B, ii) Pre-treatment scenario: IFN-containing medium was replaced after 18 h and cells were infected for 4 h either immediately after pre-treatment or after additional culturing for 72 h in IFN-free medium. The protective effect was evaluated in terms of reduction in the number of viral copies/infectious progeny, and enhanced expression of IFN-stimulated genes (ISGs). In both cell types and in both approaches, IFN-λ1 and IFN-β treatment resulted in pronounced and long-lasting antiviral effects exemplified by significantly reduced viral copy numbers and diminished infectious progeny. This was associated with strong up-regulation of multiple ISGs. However, in contrast to the IFN-β induced expression of ISGs, which decreased over time, expression of ISGs induced by IFN-λ1 was sustained or even increased over time. Here we demonstrate that the protective potential of IFN-λ1 is comparable to IFN-β. Yet, the long-lasting induction of ISGs by IFN-λ1 and most likely less incitement of side effects due to more localized expression of its receptors could make it an even more promising candidate for prophylactic treatment than IFN-β.
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- 2014
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13. Increased glutaredoxin-1 and decreased protein S-glutathionylation in sputum of asthmatics.
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Kuipers I, Louis R, Manise M, Dentener MA, Irvin CG, Janssen-Heininger YM, Brightling CE, Wouters EF, and Reynaert NL
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- Adult, Antioxidants metabolism, Female, Glutathione metabolism, Humans, Male, Middle Aged, Oxidation-Reduction, Oxidative Stress, Smoking, Asthma metabolism, Gene Expression Regulation, Glutaredoxins metabolism, Protein S metabolism, Sputum metabolism
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- 2013
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14. Interferon-β induces a long-lasting antiviral state in human respiratory epithelial cells.
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Gaajetaan GR, Geelen TH, Vernooy JH, Dentener MA, Reynaert NL, Rohde GG, Beuken EV, Grauls GE, Bruggeman CA, and Stassen FR
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- Antiviral Agents immunology, Antiviral Agents toxicity, Cell Line, Dose-Response Relationship, Drug, Epithelial Cells metabolism, Gene Expression Regulation drug effects, Humans, Interferon-beta immunology, Interferon-beta toxicity, Respiratory Mucosa metabolism, Rhinovirus drug effects, Rhinovirus immunology, Rhinovirus physiology, Antiviral Agents pharmacology, Epithelial Cells drug effects, Epithelial Cells virology, Interferon-beta pharmacology, Respiratory Mucosa drug effects, Respiratory Mucosa virology
- Abstract
Objectives: Interferon-β (IFNβ) induces strong antiviral effects and is therefore an attractive agent to prevent or reduce the incidence of virus-mediated exacerbations in asthmatic or chronic obstructive pulmonary disease (COPD) patients. We therefore investigated the effects of prophylactic IFNβ on respiratory epithelial cells infected with rhinovirus (RV)., Methods: A549 cells and primary bronchial epithelial cells (PBECs) were exposed for 18 h to IFNβ. Then, IFNβ was either removed or maintained in the supernatant for the rest of the experiment and cells were infected with RV-1B at t = 0 or 72 h after the initial exposure to IFNβ., Results: Viral RNA levels were decreased in both cell types. Furthermore, both viral RNA and infectious virus levels in the supernatant of infected A549 cells were still significantly reduced at 72 h after removal of IFNβ. This pronounced antiviral pre-treatment effect was associated with increased expression of the antiviral genes IFN-stimulated protein of MR15000 (ISG15) and Myxovirus resistance 1 (Mx1) and the effect was maintained even when IFNβ levels in the supernatant of A549 cells were undetectable., Conclusions: These data show that IFNβ has not only a strong, but also a long-lasting protective effect against RV infection of respiratory epithelium., (Copyright © 2012 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
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- 2013
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15. Decreased plasma sRAGE levels in COPD: influence of oxygen therapy.
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Gopal P, Rutten EP, Dentener MA, Wouters EF, and Reynaert NL
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- Aged, Biomarkers blood, Biomarkers metabolism, Case-Control Studies, Enzyme-Linked Immunosorbent Assay, Female, Humans, Lysine analogs & derivatives, Lysine blood, Male, Middle Aged, Oxidative Stress physiology, Receptor for Advanced Glycation End Products, Respiratory Function Tests, Time Factors, Glycation End Products, Advanced blood, Oxygen Inhalation Therapy methods, Pulmonary Disease, Chronic Obstructive blood, Pulmonary Disease, Chronic Obstructive therapy, Receptors, Immunologic blood
- Abstract
Background: Chronic obstructive pulmonary disease (COPD) is associated with systemic inflammation and oxidative stress. N(ε) -(carboxymethyl) lysine (CML), an advanced glycation end product (AGE) and the soluble decoy receptor, sRAGE, are exciting new molecules linked to oxidative stress and inflammation. Here the levels of plasma sRAGE and CML were determined and their variation in relation to lung function, external long-term oxygen therapy (LTOT) and plasma levels of inflammatory molecules in COPD evaluated., Methods: Plasma sRAGE and CML levels were measured by ELISA in 146 patients with stable COPD and 81 healthy subjects, subgrouped from a larger case-control study and matched for age, gender and pack-years smoked., Results: Decreased levels of plasma sRAGE and no significant difference in levels of plasma CML were found in patients with COPD in comparison with controls. In the total group, plasma sRAGE was positively associated with FEV(1) and forced vital capacity and negatively with pack-years smoked. In patients receiving LTOT, levels of plasma sRAGE were lower compared with those without LTOT. Only in controls, a weak correlation was found between plasma sRAGE and CML. sRAGE did not correlate with measured inflammatory markers, whereas CML was negatively correlated with fibrinogen., Conclusion: Plasma sRAGE levels are lower in patients with COPD compared with healthy control subjects, and even lower levels in patients receiving LTOT. Because sRAGE correlated with lung function only in the whole group, sRAGE can be considered a marker of COPD, but not of disease severity. A lack of clear association between sRAGE, CML and systemic inflammation is furthermore evident., (© 2012 The Authors. European Journal of Clinical Investigation © 2012 Stichting European Society for Clinical Investigation Journal Foundation.)
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- 2012
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16. Gender differences in the adipose secretome system in chronic obstructive pulmonary disease (COPD): a pivotal role of leptin.
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Breyer MK, Rutten EP, Vernooy JH, Spruit MA, Dentener MA, van der Kallen C, vanGreevenbroek MM, and Wouters EF
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- Adipose Tissue physiopathology, Aged, Biomarkers metabolism, Body Mass Index, Cardiovascular Diseases immunology, Cardiovascular Diseases physiopathology, Case-Control Studies, Female, Humans, Interleukin-6 metabolism, Leptin metabolism, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive immunology, Pulmonary Disease, Chronic Obstructive physiopathology, Sex Factors, Tumor Necrosis Factor-alpha metabolism, Adipokines metabolism, Adipose Tissue metabolism, C-Reactive Protein metabolism, Cardiovascular Diseases metabolism, Pulmonary Disease, Chronic Obstructive metabolism
- Abstract
Background: COPD is characterized by a multi-component character involving a state of low-grade systemic inflammation and an increased prevalence of cardiovascular co-morbidity. The role of circulating leptin and other adipokines in the involvement of the systemic inflammation in COPD is only studied scarcely., Objective: To investigate gender related differences in the adipokine metabolism in relation to systemic inflammatory biomarkers in clinically stable subjects with COPD., Methods: In total, 91 clinically stable COPD patients and 35 healthy control subjects, matched for body mass index (BMI) with the COPD subjects, were included. Lung function measurement and body composition were performed in patients with COPD. In the total group, plasma concentration of the adipokines (leptin, adiponectin and resistin) and systemic inflammatory biomarkers C-reactive protein (CRP), interleukin 6 (IL-6), tumor necrosis factor α (TNFα), and its soluble receptors 55 and 75 (sTNFα-R55, R75) were analyzed., Results: The COPD group was characterized by increased levels of CRP, IL-6 and leptin. Plasma adiponectin and resistin concentrations were not different between the COPD and the control group. Within the COPD group, there was a significant interaction between gender and BMI on the leptin/fat mass ratio. In COPD women, a significant correlation between leptin and CRP was present., Conclusions: In men with clinically stable COPD, leptin, adiponectin and resistin appear to be physiologically regulated, while in women, leptin metabolism is altered. Leptin secretion is increased in COPD women when compared to healthy women and compared to COPD men, and to a greater extent in overweight women with COPD., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
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17. Enhanced deposition of low-molecular-weight hyaluronan in lungs of cigarette smoke-exposed mice.
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Bracke KR, Dentener MA, Papakonstantinou E, Vernooy JH, Demoor T, Pauwels NS, Cleutjens J, van Suylen RJ, Joos GF, Brusselle GG, and Wouters EF
- Subjects
- Animals, Bronchi pathology, Bronchi physiopathology, Bronchoalveolar Lavage Fluid cytology, Collagen metabolism, Disease Models, Animal, Fibronectins metabolism, Gene Expression Regulation, Enzymologic, Glucuronosyltransferase genetics, Hyaluronan Synthases, Hyaluronoglucosaminidase genetics, Macrophages, Alveolar metabolism, Male, Mice, Mice, Inbred C57BL, Molecular Weight, Pulmonary Alveoli pathology, Pulmonary Alveoli physiopathology, Pulmonary Disease, Chronic Obstructive genetics, Pulmonary Disease, Chronic Obstructive pathology, Pulmonary Disease, Chronic Obstructive physiopathology, Pulmonary Emphysema genetics, Pulmonary Emphysema pathology, Pulmonary Emphysema physiopathology, RNA, Messenger metabolism, Time Factors, Airway Remodeling genetics, Bronchi metabolism, Hyaluronic Acid metabolism, Pulmonary Alveoli metabolism, Pulmonary Disease, Chronic Obstructive metabolism, Pulmonary Emphysema metabolism, Smoking adverse effects
- Abstract
Chronic obstructive pulmonary disease (COPD) is characterized by infiltration of inflammatory cells, destruction of lung parenchyma, and airway wall remodeling. Hyaluronan (HA) is a component of the extracellular matrix, and low-molecular-weight (LMW) HA fragments have proinflammatory capacities. We evaluated the presence of HA in alveolar and airway walls of C57BL/6 mice that were exposed to air or cigarette smoke (CS) for 4 weeks (subacute) or 24 weeks (chronic). We measured deposition of the extracellular matrix proteins collagen and fibronectin in airway walls and determined the molecular weight of HA purified from lung tissue. In addition, we studied the expression of HA-modulating genes by RT-PCR. HA staining in alveolar walls was significantly enhanced upon chronic CS exposure, whereas HA levels in the airway walls were already significantly higher upon subacute CS exposure and remained elevated upon chronic CS exposure. This differed from the deposition of collagen and fibronectin, which are only elevated at the chronic time point. In lungs of CS-exposed mice, the molecular weight of HA clearly shifted toward more LMW HA fragments. CS exposure significantly increased the mRNA expression of the HA synthase gene Has3 in total lung tissue, whereas the expression of Has1 was decreased. These in vivo studies in an experimental model of COPD show that CS exposure leads to enhanced deposition of (mostly LMW) HA in alveolar and bronchial walls by altering the expression of HA-modulating enzymes. This may contribute to airway wall remodeling and pulmonary inflammation in COPD.
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- 2010
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18. Systemic and local inflammation in asthma and chronic obstructive pulmonary disease: is there a connection?
- Author
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Wouters EF, Reynaert NL, Dentener MA, and Vernooy JH
- Subjects
- Adipose Tissue pathology, Humans, Inflammation pathology, Asthma complications, Inflammation complications, Pulmonary Disease, Chronic Obstructive complications
- Abstract
Increasing evidence indicates that chronic obstructive pulmonary disease (COPD) and probably asthma are associated with low-grade systemic inflammatory changes. In patients with COPD, systemic inflammation is considered a key factor in the pathogenesis of the multicomponent disease manifestations. Spillover of inflammatory mediators into the circulation is generally considered to be the source of this systemic inflammation. Despite this attractive hypothesis, the nature of systemic inflammation in COPD and asthma remains unclear. Available scientific data challenge the spill-over hypothesis. Interventions with biologicals such as TNF-alpha do not modify local or systemic inflammation in these inflammatory respiratory diseases. Adipose tissue-mediated inflammation is discussed as a connecting link of systemic inflammation in asthma and COPD.
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- 2009
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19. Association of glutathione-S-transferase omega haplotypes with susceptibility to chronic obstructive pulmonary disease.
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Yanbaeva DG, Wouters EF, Dentener MA, Spruit MA, and Reynaert NL
- Subjects
- Aged, Case-Control Studies, Female, Forced Expiratory Volume genetics, Genetic Association Studies, Genetic Predisposition to Disease, Genotype, Haplotypes genetics, Humans, Male, Middle Aged, Mutation, Missense, Netherlands, Point Mutation, Pulmonary Disease, Chronic Obstructive epidemiology, Pulmonary Disease, Chronic Obstructive etiology, Smoking adverse effects, Vital Capacity genetics, Glutathione Transferase genetics, Polymorphism, Single Nucleotide, Pulmonary Disease, Chronic Obstructive genetics
- Abstract
Cigarette smoking is the main risk factor for developing the inflammatory lung disease chronic obstructive pulmonary disease (COPD). Differences in susceptibility among smokers have been attributed to a genetic predisposition. A recent publication on the Framingham Heart Study found a strong association of the Asn142Asp SNP in Glutatthione-S-transferase Omega (GSTO) 2 with forced expiratory volume in the first second (FEV(1)) and forced vital capacity (FVC). FEV(1) is the main parameter reflecting the degree of airflow limitation in patients with COPD. Therefore the present study was undertaken to investigate whether the Asn142Asp polymorphism in GSTO2 occurs more frequently in patients with COPD than healthy subjects and to replicate the finding that it strongly correlates with FEV(1). Furthermore, the Ala140Asp substitution in GSTO1 was examined. Genotyping was carried out in 195 healthy controls and 355 patients with COPD. The results demonstrate that the Asn142Asp polymorphism in GSTO2 and the GSTO1140Asp/GSTO2142Asp haplotype were associated with increased risk of COPD. However, single-marker and haplotype-based analyses failed to reveal an association between lung function parameters and investigated non-synonymous coding SNPs in the GSTO genes. In conclusion, GSTO2 is a candidate gene for COPD, but is not associated with FEV(1).
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- 2009
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20. IL6 and CRP haplotypes are associated with COPD risk and systemic inflammation: a case-control study.
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Yanbaeva DG, Dentener MA, Spruit MA, Houwing-Duistermaat JJ, Kotz D, Passos VL, and Wouters EF
- Subjects
- Adult, Aged, Case-Control Studies, Female, Fibrinogen genetics, Genome-Wide Association Study, Genotype, Haplotypes, Humans, Inflammation blood, Inflammation genetics, Inflammation Mediators blood, Male, Middle Aged, Phenotype, Polymorphism, Single Nucleotide, Pulmonary Disease, Chronic Obstructive blood, C-Reactive Protein genetics, Interleukin-6 genetics, Pulmonary Disease, Chronic Obstructive genetics
- Abstract
Background: Elevated circulating levels of C-reactive protein (CRP), interleukin (IL)-6 and fibrinogen (FG) have been repeatedly associated with many adverse outcomes in patients with chronic obstructive pulmonary disease (COPD). To date, it remains unclear whether and to what extent systemic inflammation is primary or secondary in the pathogenesis of COPD. The aim of this study was to examine the association between haplotypes of CRP, IL6 and FGB genes, systemic inflammation, COPD risk and COPD-related phenotypes (respiratory impairment, exercise capacity and body composition)., Methods: Eighteen SNPs in three genes, representing optimal haplotype-tagging sets, were genotyped in 355 COPD patients and 195 healthy smokers. Plasma levels of CRP, IL-6 and FG were measured in the total study group. Differences in haplotype distributions were tested using the global and haplotype-specific statistics., Results: Raised plasma levels of CRP, IL-6 and fibrinogen were demonstrated in COPD patients. However, COPD population was very heterogeneous: about 40% of patients had no evidence of systemic inflammation (CRP < 3 mg/uL or no inflammatory markers in their top quartile). Global test for haplotype effect indicated association of CRP gene and CRP plasma levels (P = 0.0004) and IL6 gene and COPD (P = 0.003). Subsequent analysis has shown that IL6 haplotype H2, associated with an increased COPD risk (p = 0.004, OR = 4.82; 1.64 to 4.18), was also associated with very low CRP levels (p = 0.0005). None of the genes were associated with COPD-related phenotypes., Conclusion: Our findings suggest that common genetic variation in CRP and IL6 genes may contribute to heterogeneity of COPD population associated with systemic inflammation.
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- 2009
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21. Enhanced pulmonary leptin expression in patients with severe COPD and asymptomatic smokers.
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Vernooy JH, Drummen NE, van Suylen RJ, Cloots RH, Möller GM, Bracke KR, Zuyderduyn S, Dentener MA, Brusselle GG, Hiemstra PS, and Wouters EF
- Subjects
- Blotting, Western, Bronchi metabolism, Bronchi pathology, Cells, Cultured, Epithelial Cells metabolism, Female, Forced Expiratory Volume physiology, Humans, Immunohistochemistry, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive pathology, Pulmonary Disease, Chronic Obstructive physiopathology, RNA, Messenger metabolism, Vital Capacity physiology, Leptin metabolism, Lung metabolism, Pulmonary Disease, Chronic Obstructive metabolism, Receptors, Leptin metabolism, Smoking metabolism
- Abstract
Background: Chronic obstructive pulmonary disease (COPD) is characterised by an abnormal inflammatory reaction of the lungs involving activation of epithelial cells. Leptin is a pleiotropic cytokine important in the regulation of immune responses via its functional receptor Ob-Rb. This study was undertaken to test the hypothesis that severe COPD is associated with increased leptin expression in epithelial cells., Methods: Immunohistochemistry for leptin was performed on peripheral lung specimens from 20 patients with COPD (GOLD stage 4), 14 asymptomatic ex-smokers and 13 never smokers. Leptin and Ob-Rb mRNA expression were determined by rtPCR in cultured primary bronchial epithelial cells and primary type II pneumocytes. NCI-H292 and A549 cell lines were used to study functional activation of leptin signalling., Results: Leptin immunoreactivity in lung tissue was observed in bronchial epithelial cells, type II pneumocytes, macrophages (tissue/alveolar) and interstitial lymphocytic infiltrates. rtPCR analysis confirmed pulmonary leptin and Ob-Rb mRNA expression in primary bronchial epithelial cells and pneumocytes. Leptin-expressing bronchial epithelial cells and alveolar macrophages were markedly higher in patients with severe COPD and ex-smokers than in never smokers (p<0.02). Exposure of cultured primary bronchial epithelial cells to smoke resulted in increased expression of both leptin and Ob-Rb (p<0.05). Leptin induced phosphorylation of STAT3 in both NCI-H292 and A549 cells., Conclusions: Leptin expression is increased in bronchial epithelial cells and alveolar macrophages of ex-smokers with or without severe COPD compared with never smokers. A functional leptin signalling pathway is present in lung epithelial cells.
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- 2009
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22. Effect of infliximab on local and systemic inflammation in chronic obstructive pulmonary disease: a pilot study.
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Dentener MA, Creutzberg EC, Pennings HJ, Rijkers GT, Mercken E, and Wouters EF
- Subjects
- Acute-Phase Proteins metabolism, Adult, Aged, Aged, 80 and over, Biomarkers metabolism, C-Reactive Protein metabolism, Cachexia metabolism, Carrier Proteins metabolism, Cytokines metabolism, Double-Blind Method, Female, Fibrinogen metabolism, Humans, Infliximab, Intercellular Adhesion Molecule-1 metabolism, Male, Membrane Glycoproteins metabolism, Middle Aged, Peroxidase metabolism, Pilot Projects, Receptors, Tumor Necrosis Factor metabolism, Severity of Illness Index, Uteroglobin metabolism, Anti-Inflammatory Agents therapeutic use, Antibodies, Monoclonal therapeutic use, Pulmonary Disease, Chronic Obstructive drug therapy, Pulmonary Disease, Chronic Obstructive metabolism
- Abstract
Background: Chronic obstructive pulmonary disease (COPD) with cachexia is characterized by inflammation reflected by increased levels of tumor necrosis factor-alpha (TNF-alpha)., Objectives: In this study, infliximab, an anti-TNF-alpha antibody, was evaluated for its effects on systemic (plasma) and local (exhaled breath condensate, EBC) inflammation in cachectic patients with COPD. Also, baseline levels of new inflammatory markers were compared to control subjects., Methods: Sixteen cachectic patients with moderate to severe COPD were examined for inflammatory status at baseline and compared to 25 control subjects. Patients were randomized (1:1) to receive infliximab (5 mg/kg) or placebo at weeks 0, 2 and 6. Patients were evaluated at weeks 8 and 12 and followed through week 26., Results: EBC analysis revealed increased levels of several novel inflammatory markers, including macrophage migration inhibitory factor, IL-12, RANTES and sICAM-1, in patients with COPD compared to controls. EBC levels of inflammatory markers were unchanged in patients receiving infliximab. In addition, systemic levels of acute-phase proteins (C-reactive protein, fibrinogen and lipopolysaccharide-binding protein), IL-6 and soluble TNF receptor (sTNFR) 55 had not changed at weeks 8 or 12. Small increases in circulating levels of sTNFR75, myeloperoxidase and Clara cell protein 16 were seen at week 8, but not at week 12., Conclusions: In this small study, infliximab did not produce an observable decrease in local inflammation in cachectic patients with COPD and had minor effects on systemic inflammation. The detection of new inflammatory markers in EBC can help to further characterize local inflammatory processes in COPD., (Copyright 2008 S. Karger AG, Basel.)
- Published
- 2008
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23. Systemic inflammation in chronic obstructive pulmonary disease: the role of exacerbations.
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Wouters EF, Groenewegen KH, Dentener MA, and Vernooy JH
- Subjects
- Adiponectin metabolism, C-Reactive Protein metabolism, Endothelium, Vascular physiopathology, Hemostasis physiology, Humans, Immunity, Innate, Inflammation immunology, Leptin metabolism, Pulmonary Disease, Chronic Obstructive immunology, Inflammation physiopathology, Pulmonary Disease, Chronic Obstructive physiopathology
- Abstract
The systemic manifestations of chronic obstructive pulmonary disease (COPD) exacerbations are recognized, but our understanding of their etiology and importance is lacking largely due to the small number of systematic and longitudinal studies. Most of the systemic manifestations are likely the result of inflammatory processes. Serum biomarkers, such as various cytokines, adipokines, C-reactive protein, and coagulation factors, are elevated during exacerbations. Our understanding of the systemic manifestations can be greatly enhanced if we integrate what is known about the basic science of systemic mediators with the translational science of their role in COPD exacerbations. Many overlapping connections and promising avenues of future research come to light with such a viewpoint.
- Published
- 2007
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24. Longitudinal follow-up of systemic inflammation after acute exacerbations of COPD.
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Groenewegen KH, Dentener MA, and Wouters EF
- Subjects
- Aged, Antimicrobial Cationic Peptides metabolism, Antioxidants metabolism, Blood Proteins metabolism, Case-Control Studies, Disease Progression, Enzyme-Linked Immunosorbent Assay, Female, Forced Expiratory Volume physiology, Hospitalization, Humans, Interleukins metabolism, Longitudinal Studies, Male, Membrane Proteins metabolism, Pulmonary Disease, Chronic Obstructive drug therapy, Pulmonary Disease, Chronic Obstructive physiopathology, Sputum microbiology, Time Factors, Vital Capacity physiology, Inflammation blood, Inflammation etiology, Inflammation Mediators metabolism, Pulmonary Disease, Chronic Obstructive complications
- Abstract
Background: Acute exacerbations are important in the clinical course of COPD, yet the underlying mechanisms are poorly understood. Systemic inflammation is now considered as an important component in the disease process. In this study we evaluated longitudinally the systemic inflammation during hospital treatment for acute exacerbation and after clinical recovery., Methods: Blood was collected on day 0, 1, 4 and 8 in 21 patients admitted for an acute exacerbation of COPD and at 1 month, 3 months and 6 months after discharge. Systemic inflammation was determined by measurement of the pro-inflammatory markers interleukin (IL)-6, soluble tumor necrosis factor (TNF) receptors sTNFR55 and sTNFR75, the anti-inflammatory mediator sIL-1RII, and bactericidal permeability increasing protein (BPI) as a marker of neutrophil activation. In addition, plasma level of Trolox antioxidant capacity (TEAC) was determined. Healthy age-matched controls were measured for the same markers at one time-point., Results: All inflammatory markers analyzed were elevated on first day of admission for exacerbation of COPD, as compared to healthy controls. During treatment, levels of IL-6, and sTNFR75 rapidly decreased, whereas sTNFR55 and BPI remained elevated. Moreover, sIL-1RII and TEAC increased during first 8 days of treatment. In the stable condition all inflammatory markers returned to values comparable to healthy controls, with the exception of BPI, which remained persistently elevated compared to healthy controls., Conclusion: This study clearly demonstrates upregulation of systemic inflammation in acute exacerbations of COPD. Attenuation of systemic inflammation may offer new perspectives in the management of COPD patients to reduce the burden of exacerbations.
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- 2007
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25. Systemic effects of smoking.
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Yanbaeva DG, Dentener MA, Creutzberg EC, Wesseling G, and Wouters EF
- Subjects
- Atherosclerosis etiology, Atherosclerosis physiopathology, Blood Coagulation Disorders etiology, Blood Coagulation Disorders physiopathology, Chronic Disease, Endothelium, Vascular physiopathology, Environment, Genetic Predisposition to Disease, Hemostasis physiology, Humans, Inflammation physiopathology, Oxidative Stress physiology, Smoking genetics, Inflammation etiology, Smoking adverse effects
- Abstract
Smoking is one of the major lifestyle factors influencing the health of human beings. Life-long cigarette smokers have a higher prevalence of common diseases such as atherosclerosis and COPD with significant systemic impact. The present review evaluates current knowledge concerning possible pathways through which cigarette smoking can affect human health, with special focus on extrapulmonary effects. Long-term smoke exposure can result in systemic oxidants-antioxidants imbalance as reflected by increased products of lipid peroxidation and depleted levels of antioxidants like vitamins A and C in plasma of smokers. A low-grade systemic inflammatory response is evident in smokers as confirmed by numerous population-based studies: elevated levels of C-reactive protein (CRP), fibrinogen, and interleukin-6, as well as increased counts of WBC have been reported. Furthermore, rheologic, coagulation and endothelial function markers like hematocrit, blood and/or plasma viscosity, fibrin d-dimer, circulating adhesion molecules (intracellular adhesion molecule-1, selectins), tissue plasminogen activator antigen, and plasminogen activator inhibitor type I are altered in chronic cigarette smokers. Although most of smoking-induced changes are reversible after quitting, some inflammatory mediators like CRP are still significantly raised in ex-smokers up to 10 to 20 years after quitting, suggesting ongoing low-grade inflammatory response persisting in former smokers. New longitudinal epidemiologic and genetic studies are required to evaluate the role of smoking itself and possible gene/environment interplay in initiation and development of smoking-induced common diseases affecting humans.
- Published
- 2007
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26. Tumor necrosis factor inhibitors for rheumatoid arthritis.
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Dentener MA and Wouters EF
- Subjects
- Arthritis, Rheumatoid drug therapy, Humans, Infliximab, Anti-Inflammatory Agents therapeutic use, Antibodies, Monoclonal therapeutic use, Pulmonary Disease, Chronic Obstructive drug therapy, Tumor Necrosis Factor-alpha antagonists & inhibitors
- Published
- 2006
27. Human bancroftian filariasis: immunological markers of morbidity and infection.
- Author
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Satapathy AK, Sartono E, Sahoo PK, Dentener MA, Michael E, Yazdanbakhsh M, and Ravindran B
- Subjects
- Acute-Phase Proteins immunology, Adolescent, Adult, Aged, Aged, 80 and over, Animals, Biomarkers blood, Carrier Proteins blood, Carrier Proteins immunology, Child, Cytokines immunology, Elephantiasis, Filarial blood, Female, Humans, Intercellular Adhesion Molecule-1 blood, Intercellular Adhesion Molecule-1 immunology, Interleukins blood, Interleukins immunology, Male, Membrane Glycoproteins blood, Membrane Glycoproteins immunology, Middle Aged, Morbidity, Receptors, Tumor Necrosis Factor blood, Receptors, Tumor Necrosis Factor immunology, Receptors, Tumor Necrosis Factor, Type I blood, Receptors, Tumor Necrosis Factor, Type I immunology, Receptors, Tumor Necrosis Factor, Type II blood, Receptors, Tumor Necrosis Factor, Type II immunology, Tumor Necrosis Factor-alpha blood, Tumor Necrosis Factor-alpha immunology, Cytokines blood, Elephantiasis, Filarial immunology, Wuchereria bancrofti immunology
- Abstract
Induction of host cytokines plays a critical role in infection as well as disease in human filariasis. Measurements of such molecules in plasma could be used as windows of markers both for understanding the pathogenesis of the disease and for identifying markers of morbidity. Eight inflammatory and non-inflammatory host molecules in circulation were quantified in 207 subjects in filariasis endemic area of Orissa, India. IL-6, IL-8, IL-10, TNF-alpha, TNFR-I, TNFR-II, LBP and sICAM-1 were quantified by immunoassays and were analyzed by multivariate exploratory data analysis methods followed by multivariate analysis of variance. Raised levels of IL-6 and IL-8 emerged as markers of acute as well as chronic disease, while increased TNF-alpha was a feature found only in acute filariasis. Decreased sICAM-1 was a feature found only in asymptomatic subjects with filarial infection. There was a dichotomy in plasma levels of two TNF receptors between infected subjects and patients with filarial disease. Since plasma levels of these cytokines are often determined by host genetics, studies on cytokine genetic polymorphisms could offer new insights into the relationship between infection and disease in human lymphatic filariasis.
- Published
- 2006
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28. Differences in local versus systemic TNFalpha production in COPD: inhibitory effect of hyaluronan on LPS induced blood cell TNFalpha release.
- Author
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Dentener MA, Louis R, Cloots RH, Henket M, and Wouters EF
- Subjects
- Adult, Aged, Bronchitis, Case-Control Studies, Enzyme-Linked Immunosorbent Assay, Female, Forced Expiratory Volume physiology, Humans, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive physiopathology, Sputum cytology, Sputum drug effects, Tumor Necrosis Factor-alpha antagonists & inhibitors, Vital Capacity physiology, Blood Cells metabolism, Hyaluronic Acid pharmacology, Lipopolysaccharides pharmacology, Pulmonary Disease, Chronic Obstructive metabolism, Tumor Necrosis Factor-alpha biosynthesis
- Abstract
Background: Chronic obstructive pulmonary disease (COPD) is characterised by both airway inflammation and systemic changes. To elucidate the relationship between local and systemic inflammation, tumour necrosis factor alpha (TNFalpha) production by sputum cells and blood cells of patients with COPD and controls was compared and the effect of the extracellular matrix compound hyaluronan (HA) on TNFalpha release was studied., Methods: Four study groups were included: 10 steroid free COPD patients, 8 steroid treated patients, 10 healthy smokers, and 11 healthy non-smokers. Sputum cells and blood were incubated for 24 hours with or without lipopolysaccharide (LPS) in the absence or presence of HA (122 kDa or HMW fragment). TNFalpha was measured by ELISA., Results: Sputum cells produced spontaneously high levels of TNFalpha but were unresponsive to LPS. Sputum cells from COPD patients (both steroid free and steroid treated) produced significantly less TNFalpha than cells from healthy non-smoking subjects (p=0.017 and p=0.001, respectively). In contrast, blood cells produced TNFalpha only in response to LPS. No differences were observed in TNFalpha production by blood cells between the patient groups and the control groups. HA (both fragments) partially blocked LPS (1 ng/ml) induced TNFalpha release by blood cells from all study groups, whereas TNFalpha production by sputum cells was not influenced by HA., Conclusion: These data indicate a difference between local and systemic TNFalpha production. Sputum cells of patients with COPD produced less TNFalpha than controls, which could contribute to impaired local defence. An inhibitory effect of HA on TNFalpha release in blood cells was observed which was similar in both patients and controls.
- Published
- 2006
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29. Patterns of inflammation and the use of reversibility testing in smokers with airway complaints.
- Author
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Chavannes NH, Vernooy JH, Schermer TR, Jacobs JA, Dentener MA, van Weel C, van Schayck OC, and Wouters EF
- Subjects
- Adult, Aged, Asthma etiology, Asthma immunology, Biomarkers blood, Bronchitis etiology, Bronchitis immunology, Case-Control Studies, Eosinophilia blood, Female, Humans, Inflammation, Male, Middle Aged, Primary Health Care, Pulmonary Disease, Chronic Obstructive etiology, Pulmonary Disease, Chronic Obstructive immunology, Regression Analysis, Sputum cytology, Asthma diagnosis, Biomarkers analysis, Bronchitis diagnosis, Eosinophilia diagnosis, Pulmonary Disease, Chronic Obstructive diagnosis, Smoking adverse effects
- Abstract
Background: Although both smoking and respiratory complaints are very common, tools to improve diagnostic accuracy are scarce in primary care. This study aimed to reveal what inflammatory patterns prevail in clinically established diagnosis groups, and what factors are associated with eosinophilia., Method: Induced sputum and blood plasma of 59 primary care patients with COPD (n = 17), asthma (n = 11), chronic bronchitis (CB, n = 14) and smokers with no respiratory complaints ('healthy smokers', n = 17) were collected, as well as lung function, smoking history and clinical work-up. Patterns of inflammatory markers per clinical diagnosis and factors associated with eosinophilia were analyzed by multiple regression analyses, the differences expressed in odds ratios (OR) with 95% confidence intervals., Results: Multivariately, COPD was significantly associated with raised plasma-LBP (OR 1.2 [1.04-1.37]) and sTNF-R55 in sputum (OR 1.01 [1.001-1.01]), while HS expressed significantly lowered plasma-LBP (OR 0.8 [0.72-0.95]). Asthma was characterized by higher sputum eosinophilic counts (OR 1.3 [1.05-1.54]), while CB showed a significantly higher proportion of sputum lymphocytic counts (OR 1.5 [1.12-1.9]). Sputum eosinophilia was significantly associated with reversibility after adjusting for smoking, lung function, age, gender and allergy., Conclusion: Patterns of inflammatory markers in a panel of blood plasma and sputum cells and mediators were discernable in clinical diagnosis groups of respiratory disease. COPD and so-called healthy smokers showed consistent opposite associations with plasma LBP, while chronic bronchitics showed relatively predominant lymphocytic inflammation compared to other diagnosis groups. Only sputum eosinophilia remained significantly associated with reversibility across the spectrum of respiratory disease in smokers with airway complaints.
- Published
- 2006
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30. Systemic inflammation in COPD: is genetic susceptibility a key factor?
- Author
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Yanbaeva DG, Dentener MA, Creutzberg EC, and Wouters EF
- Subjects
- C-Reactive Protein genetics, C-Reactive Protein physiology, Humans, Interleukin-6 analysis, Interleukin-6 genetics, Lymphotoxin-alpha genetics, Polymorphism, Genetic, Receptors, Tumor Necrosis Factor genetics, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha physiology, Genetic Predisposition to Disease, Inflammation genetics, Pulmonary Disease, Chronic Obstructive genetics
- Abstract
COPD is a multicomponent disease characterized by abnormal inflammatory response of the lungs to noxious particles that is accompanied by systemic effects like weight loss, muscle wasting, reduced functional capacity and impaired health status. A persistent low-grade systemic inflammatory response reflected by enhanced levels of acute phase proteins like C-reactive protein (CRP) and pro-inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, is present in part of the COPD population. The production of inflammatory proteins is partly genetically determined. Several studies have shown that polymorphisms within genes coding for these inflammatory mediators may modulate systemic inflammatory responses. Among all of these genes, the TNF family (TNF-alpha, lymphotoxin (LT)-alph and their receptors TNF-R55 and TNF-R75), interleukin (IL)-6 and CRP gene polymorphisms are the most prominent candidates. However, large carefully designed studies in well-characterized COPD cohorts are required to unravel the exact role of genetic background in the systemic component of this disease.
- Published
- 2006
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31. Rapid pulmonary expression of acute-phase reactants after local lipopolysaccharide exposure in mice is followed by an interleukin-6 mediated systemic acute-phase response.
- Author
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Vernooy JH, Reynaert N, Wolfs TG, Cloots RH, Haegens A, de Vries B, Dentener MA, Buurman WA, and Wouters EM
- Subjects
- Animals, Carrier Proteins blood, Carrier Proteins genetics, Liver metabolism, Male, Membrane Glycoproteins blood, Membrane Glycoproteins genetics, Mice, Orosomucoid analysis, Orosomucoid genetics, Trachea drug effects, alpha 1-Antitrypsin analysis, alpha 1-Antitrypsin genetics, Acute-Phase Proteins genetics, Acute-Phase Reaction, Interleukin-6 physiology, Lipopolysaccharides toxicity, Lung metabolism
- Abstract
This study investigated local and systemic innate immune responses in lipopolysaccharide (LPS)-induced lung inflammation in mice. Intratracheal LPS exposure resulted in increased pulmonary mRNA expression for acute-phase reactants (APRs) alpha(1)-antitrypsin (alpha(1)-AT), alpha(1)-acid glycoprotein (AGP), and LPS-binding protein (LBP) from 4 hours post exposure. Although pulmonary serum amyloid P component (SAP) mRNA was not increased, systemic levels of SAP, AGP, and LBP were elevated from 24 hours post exposure. Systemic APRs increase was associated with hepatic mRNA expression. As in vivo neutralization of interleukin (IL)-6, but not tumor necrosis factor (TNF)-alpha, fully ablated hepatic APR mRNA expression, IL-6 may act as signaling molecule between lung and liver. In conclusion, pulmonary LPS exposure induced rapid APR expression in lung, which precedes IL-6-mediated systemic elevation of APRs associated with hepatic APRs expression.
- Published
- 2005
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32. Lipopolysaccharide-binding protein is produced in the epididymis and associated with spermatozoa and prostasomes.
- Author
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Malm J, Nordahl EA, Bjartell A, Sørensen OE, Frohm B, Dentener MA, and Egesten A
- Subjects
- Acute-Phase Proteins genetics, Carrier Proteins genetics, Epididymis cytology, Epithelial Cells immunology, Gene Expression, Humans, Male, Membrane Glycoproteins genetics, RNA, Messenger analysis, RNA, Messenger metabolism, Secretory Vesicles chemistry, Semen immunology, Sperm Head immunology, Sperm Tail immunology, Spermatozoa immunology, Acute-Phase Proteins analysis, Acute-Phase Proteins biosynthesis, Carrier Proteins analysis, Carrier Proteins biosynthesis, Epididymis metabolism, Membrane Glycoproteins analysis, Membrane Glycoproteins biosynthesis, Prostate metabolism, Spermatozoa chemistry
- Abstract
Lipopolysaccharide-binding protein (LBP) is an acute phase protein known to play a central role in the defense against Gram-negative bacteria. It binds lipopolysaccharides of Gram-negative bacteria and, after binding to CD14, the complex signals through Toll-like receptor (TLR)-4, eliciting host-defense responses, such as cytokine production, in inflammatory cells. The present study demonstrates constitutive expression of the gene encoding lipopolysaccharide-binding protein in the epithelium of the human epididymis by in situ hybridization. Using immunohistochemistry lipopolysaccharide-binding protein was shown to be present in the same cells and also attached to the heads and tails of spermatozoa. Cell-free seminal plasma, lysed spermatozoa and lysed prostasomes were subjected to Western blot; all showed immunoreactive bands corresponding to the size of lipopolysaccharide-binding protein. Gel filtration demonstrated that lipopolysaccharide-binding protein colocalizes with prostasomes. The concentration of lipopolysacharide-binding protein in seminal plasma was 127+/-42ng/mL (mean+/-S.D.; range 73-215ng/mL). Taken together, our results suggest roles for lipopolysaccharide-binding protein during human reproduction.
- Published
- 2005
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33. Enhanced levels of hyaluronan in lungs of patients with COPD: relationship with lung function and local inflammation.
- Author
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Dentener MA, Vernooy JH, Hendriks S, and Wouters EF
- Subjects
- Adult, Aged, Cell Adhesion Molecules metabolism, Female, Forced Expiratory Volume physiology, GPI-Linked Proteins, Glucuronosyltransferase metabolism, Humans, Hyaluronan Synthases, Hyaluronoglucosaminidase metabolism, Lung physiopathology, Male, Middle Aged, Pneumonia physiopathology, Pulmonary Disease, Chronic Obstructive etiology, Pulmonary Disease, Chronic Obstructive physiopathology, RNA analysis, Reverse Transcriptase Polymerase Chain Reaction methods, Sputum metabolism, Hyaluronic Acid metabolism, Lung metabolism, Pneumonia metabolism, Pulmonary Disease, Chronic Obstructive metabolism
- Abstract
Background: Chronic inflammation and airway remodelling are characteristics of chronic obstructive pulmonary disease (COPD). Hyaluronan (HA) is an extracellular matrix compound with proinflammatory activity. HA levels in induced sputum from patients with COPD were measured and related to local inflammation. The expression of hyaluronan synthase 2 (HAS2) and hyaluronidase 2 (HYAL2) was analysed in lung tissue., Methods: Sputum was obtained from 18 patients with COPD (forced expiratory volume in 1 second (FEV(1)) 62% predicted (range 20-76)) and 14 healthy smokers. HA and inflammatory markers were measured using ELISA assays. Lung sections were obtained from five patients with severe COPD (FEV(1) <30%) and from five smokers, and mRNA levels of HAS2 and HYAL2 were analysed by polymerase chain reaction., Results: HA levels were significantly higher in the sputum from patients with COPD than controls. The COPD population appeared to consist of two subpopulations with either high or moderate HA levels. The subgroup of patients with high HA levels had lower FEV(1) than the moderate HA group. In addition, neutrophil influx and levels of interleukin-8, and the soluble tumour necrosis factor receptors R55 and R75 were significantly higher in patients with high HA levels than in those with moderate HA levels and controls. Semiquantitative analysis revealed enhanced expression of HYAL2 in lung tissue of patients with severe COPD compared with control subjects., Conclusion: These data indicate a relationship between HA levels, local inflammation and severity of disease, and suggest enhanced breakdown of HA in the lungs of patients with COPD.
- Published
- 2005
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34. Leptin as local inflammatory marker in COPD.
- Author
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Broekhuizen R, Vernooy JH, Schols AM, Dentener MA, and Wouters EF
- Subjects
- Adult, Aged, Anthropometry, Biomarkers metabolism, C-Reactive Protein metabolism, Forced Expiratory Volume, Humans, Inflammation Mediators metabolism, Leptin analysis, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive physiopathology, Sputum metabolism, Tumor Necrosis Factor-alpha metabolism, Vital Capacity, Leptin metabolism, Pulmonary Disease, Chronic Obstructive metabolism
- Abstract
Introduction: Chronic inflammation of the lung is a characteristic finding in chronic obstructive pulmonary disease (COPD). Leptin is a pleiotropic cytokine thought to play a role in host response to inflammation. As recent studies have shown that leptin receptors are present in the lung, this study aimed to determine if leptin is detectable in induced sputum of COPD patients and if there is a relationship between leptin and other inflammatory markers in sputum., Methods: Sputum was induced in 14 male patients with moderate COPD (FEV1: 56 (15) % pred.). Leptin, total tumour necrosis factor (TNF)-alpha, and C-reactive protein (CRP) were analyzed in induced sputum supernatant by ELISA. Leptin was also determined in EDTA plasma., Results: Leptin was detectable in induced sputum of 10 COPD patients. A significant relationship was found between sputum leptin and CRP (r = 0.943, P < 0.001) and total TNF-alpha (r = 0.690, P < 0.01). Plasma leptin and sputum leptin were inversely correlated (r = -0.643, P < 0.01)., Conclusion: The present study demonstrated that leptin is detectable in induced sputum of patients with moderate COPD and is related to other inflammatory markers. The observed correlations between leptin and inflammatory markers in sputum may indicate that leptin is involved in the local inflammatory response in COPD.
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- 2005
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35. Childhood asthma: exhaled markers of airway inflammation, asthma control score, and lung function tests.
- Author
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Rosias PP, Dompeling E, Dentener MA, Pennings HJ, Hendriks HJ, Van Iersel MP, and Jöbsis Q
- Subjects
- Adolescent, Albumins analysis, Asthma immunology, C-Reactive Protein analysis, Carbon Monoxide analysis, Case-Control Studies, Child, Cytokines analysis, Enzyme-Linked Immunosorbent Assay, Exhalation, Humans, Hydrogen-Ion Concentration, Intercellular Adhesion Molecule-1 analysis, Interleukin-6 analysis, Interleukin-8 analysis, Nitric Oxide analysis, Patient Selection, Receptors, Tumor Necrosis Factor, Type II analysis, Respiratory Function Tests, Surveys and Questionnaires, Tumor Necrosis Factor-alpha analysis, Asthma diagnosis, Breath Tests, Inflammation Mediators analysis
- Abstract
Exhaled markers of airway inflammation become increasingly important in the management of childhood asthma. The aims of the present study are: 1) to compare exhaled markers of inflammation (nitric oxide, carbon monoxide, and acidity of breath condensate) with conventional asthma measures (lung function tests and asthma control score) in childhood asthma; and 2) to investigate the detectability of albumin, CRP, IL-6, IL-8, TNF-alpha, sICAM-1, and sTNF-R75 in the exhaled breath condensate (EBC) of asthmatic children. Thirty-two children with mild to moderate persistent asthma and healthy controls aged 6-12 years were studied. We measured exhaled NO and CO, and subsequently EBC was collected. Inflammatory mediators in EBC were measured using an enzyme-linked immunosorbent assay. Respiratory symptoms and asthma control were assessed using the asthma control questionnaire (ACQ) of Juniper et al. (Eur Respir J 1999;14:902-907). Exhaled NO showed a significant correlation with exhaled CO (r = 0.59, P < 0.05) and FEV1 (r = -0.59, P < 0.05), but not with ACQ score (r = 0.48, P = 0.06). Exhaled CO was correlated with prebronchodilator FEV1 (r = -0.45, P < 0.05), but not with asthma control (r = 0.18, P = 0.35). Acidity of EBC was significantly lower in asthmatic children than in healthy controls (P < 0.05), but did not correlate with any of the conventional asthma measures. We were not able to demonstrate the presence of CRP, IL-6, IL-8, TNF-alpha, sICAM-1, and sTNF-R75 in EBC. Albumin was found in two EBC samples of asthmatic children. We conclude that exhaled NO had a better correlation with lung function parameters and asthma control than exhaled CO and acidity of EBC, in mild to moderate persistent childhood asthma. However, exhaled NO, CO, and deaerated pH of EBC did not differ between asthmatic children and controls, possibly because of a too homogeneous and well-controlled study population. To further evaluate the clinical utility of exhaled markers in monitoring childhood asthma, more studies are required on a wider range of asthma severity, and preferably with repeated measurements of markers and of asthma control., (Copyright 2004 Wiley-Liss, Inc.)
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- 2004
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36. Chronic obstructive pulmonary disease is associated with the -1055 IL-13 promoter polymorphism.
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van der Pouw Kraan TC, Küçükaycan M, Bakker AM, Baggen JM, van der Zee JS, Dentener MA, Wouters EF, and Verweij CL
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- Adult, Aged, Female, Humans, Immunoglobulin E blood, Male, Middle Aged, Polymorphism, Genetic, Genetic Predisposition to Disease, Interleukin-13 genetics, Promoter Regions, Genetic, Pulmonary Disease, Chronic Obstructive genetics
- Abstract
IL-13 is strongly implicated in the development of asthma and chronic obstructive pulmonary disease (COPD). We previously identified an IL-13 promoter polymorphism (-1055 C to T) that is associated with allergic asthma. We now report an increased frequency of the -1055 T allele in COPD patients compared to healthy controls (P=0.002) and compared to a second control group consisting of smoking individuals with normal lung function (P=0.01). A closely linked IL-13 exon polymorphism is present at normal allelic frequencies (P=0.3 and 0.4, respectively). In addition, we observed a normal distribution of two IL-4 polymorphisms at positions -590 and +33 (P=0.2 and 0.9, respectively). These results could implicate a functional role for the IL-13 promoter polymorphism in the enhanced risk to develop COPD.
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- 2002
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37. Local and systemic inflammation in patients with chronic obstructive pulmonary disease: soluble tumor necrosis factor receptors are increased in sputum.
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Vernooy JH, Küçükaycan M, Jacobs JA, Chavannes NH, Buurman WA, Dentener MA, and Wouters EF
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- Aged, Etanercept, Female, Humans, Male, Middle Aged, Pneumonia blood, Pulmonary Disease, Chronic Obstructive blood, Smoking blood, Antineoplastic Agents analysis, Immunoglobulin G analysis, Immunologic Factors analysis, Interleukin-8 analysis, Pneumonia etiology, Pulmonary Disease, Chronic Obstructive complications, Receptors, Tumor Necrosis Factor analysis, Smoking adverse effects, Sputum chemistry, Tumor Necrosis Factor-alpha analysis
- Abstract
Chronic obstructive pulmonary disease (COPD) is characterized by significant chronic inflammation in the pulmonary compartment as well as in the circulation. This study aimed to elucidate the relationship between local and systemic inflammation in smoking-induced COPD by assessing levels of soluble (s) tumor necrosis factor (TNF) receptors, TNF-alpha, and interleukin-8 (IL-8) in induced sputum and in plasma. Sputum induction was performed in 18 subjects with COPD (FEV(1) 56% predicted) and 17 healthy smokers (FEV(1) 99% predicted). Patients with COPD showed significantly higher percentages of neutrophils and levels of sTNF-R55 and IL-8 in sputum as compared with control subjects, whereas sputum sTNF-R75 levels tended to be higher in COPD. Sputum TNF-alpha levels were similar in both groups. When comparing sTNF receptors in sputum and plasma, no direct correlations were found despite elevation of circulating sTNF-R75 levels in patients with COPD. In addition, sputum sTNF receptors were inversely related to the FEV(1) in patients with COPD, whereas circulating sTNF receptors were not, suggesting different regulation of inflammation in the pulmonary and systemic compartment. When subjects were divided according to their current smoking status, levels of sTNF-R55, sTNF-R75, and IL-8 in sputum were significantly elevated in ex-smoking versus currently smoking patients with COPD, suggesting ongoing inflammation in airways and circulation of patients with COPD after smoking cessation.
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- 2002
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38. Tumor necrosis factor-alpha +489G/A gene polymorphism is associated with chronic obstructive pulmonary disease.
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Küçükaycan M, Van Krugten M, Pennings HJ, Huizinga TW, Buurman WA, Dentener MA, and Wouters EF
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- Adult, Aged, Aged, 80 and over, Alleles, Female, Genotype, Humans, Male, Middle Aged, Netherlands epidemiology, Pulmonary Disease, Chronic Obstructive epidemiology, Pulmonary Emphysema diagnostic imaging, Pulmonary Emphysema epidemiology, Pulmonary Emphysema genetics, Radiography, Adenine, Guanine, Polymorphism, Genetic genetics, Pulmonary Disease, Chronic Obstructive genetics, Tumor Necrosis Factor-alpha genetics
- Abstract
Background: Chronic obstructive pulmonary disease (COPD) is characterized by a chronic inflammatory process, in which the pro-inflammatory cytokine Tumor Necrosis Factor (TNF)-alpha is considered to play a role. In the present study the putative involvement of TNF-alpha gene polymorphisms in pathogenesis of COPD was studied by analysis of four TNF-alpha gene polymorphisms in a Caucasian COPD population., Methods: TNF-alpha gene polymorphisms at positions -376G/A, -308G/A, -238G/A, and +489G/A were examined in 169 Dutch COPD patients, who had a mean forced expiratory volume in one second (FEV1) of 37 +/- 13%, and compared with a Dutch population control group of 358 subjects., Results: The data showed that the TNF-alpha +489G/A genotype frequency tended to be different in COPD patients as compared to population controls, which was due to an enhanced frequency of the GA genotype. In line herewith, carriership of the minor allele was associated with enhanced risk of development of COPD (odds ratio = 1.9, p = 0.009). The other TNF-alpha gene polymorphisms studied revealed no discrimination between patients and controls. No differences in the examined four TNF-alpha polymorphisms were found between subtypes of COPD, which were stratified for the presence of radiological emphysema. However, comparison of the COPD subtypes with controls showed a significant difference in the TNF-alpha +489G/A genotype in patients without radiological emphysema (chi2-test: p < 0.025 [Bonferroni adjusted]), while no differences between COPD patients with radiological emphysema and controls were observed., Conclusion: Based on the reported data, it is concluded that COPD, and especially a subgroup of COPD patients without radiological emphysema, is associated with TNF-alpha +489G/A gene polymorphism.
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- 2002
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39. Long-term intratracheal lipopolysaccharide exposure in mice results in chronic lung inflammation and persistent pathology.
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Vernooy JH, Dentener MA, van Suylen RJ, Buurman WA, and Wouters EF
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- Animals, Antigens, CD19 biosynthesis, CD4-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes metabolism, Cytokines biosynthesis, Escherichia coli metabolism, Immunohistochemistry, Lung pathology, Lymphocytes metabolism, Macrophages metabolism, Male, Mice, Mucous Membrane metabolism, Pulmonary Alveoli immunology, Pulmonary Alveoli metabolism, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Inflammation, Lipopolysaccharides pharmacology, Lung immunology, Trachea metabolism
- Abstract
Lipopolysaccharide (LPS), a major proinflammatory glycolipid component of the gram-negative bacterial cell wall, is one of the agents ubiquitously present as contaminant on airborne particles, including air pollution, organic dusts, and cigarette smoke. Chronic exposure to significant levels of LPS is reported to be associated with the development and/or progression of many types of lung diseases, including asthma, chronic bronchitis, and progressive irreversible airflow obstruction, that are all characterized by chronic inflammatory processes in the lung. In the present study, pathologic effects of long-term LPS exposure to the lung were investigated in detail. To this end, a murine model in which mice were exposed to repeated intratracheal instillation of Escherichia coli LPS was developed. We show that long-term LPS instillation in mice results in persistent chronic pulmonary inflammation, characterized by peribronchial and perivascular lymphocytic aggregates (CD4(+), CD8(+), and CD19(+)), parenchymal accumulation of macrophages and CD8(+) T cells, and altered cytokine expression. Furthermore, airway and alveolar alterations such as mucus cell metaplasia, airway wall thickening, and irreversible alveolar enlargement accompanied the chronic inflammatory response. Interestingly, the observed inflammatory and pathologic changes mimic changes observed in human subjects with chronic inflammatory lung diseases, especially chronic obstructive pulmonary disease (COPD), suggesting that this murine model could be applicable to dissect the role of inflammation in the pathogenesis of these disease conditions.
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- 2002
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40. Systemic anti-inflammatory mediators in COPD: increase in soluble interleukin 1 receptor II during treatment of exacerbations.
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Dentener MA, Creutzberg EC, Schols AM, Mantovani A, van't Veer C, Buurman WA, and Wouters EF
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- Adult, Aged, Aged, 80 and over, Antigens, CD analysis, Carrier Proteins, Chronic Disease, Cyclic AMP Receptor Protein analysis, DNA-Binding Proteins analysis, Female, Forced Expiratory Volume physiology, Humans, Lung Diseases, Obstructive physiopathology, Male, Middle Aged, Receptors, Interleukin-1 Type II, Receptors, Tumor Necrosis Factor analysis, Receptors, Tumor Necrosis Factor, Type I, Receptors, Tumor Necrosis Factor, Type II, Signal Transduction, Transcription Factors, Vital Capacity physiology, Lung Diseases, Obstructive metabolism, Receptors, Interleukin-1 metabolism
- Abstract
Background: The aim of this study was to test the hypothesis that the chronic inflammatory process present in chronic obstructive pulmonary disease (COPD) is due to a defective endogenous anti-inflammatory mechanism., Methods: Systemic levels of the anti-inflammatory mediators soluble interleukin 1 receptor II (sIL-1RII), soluble tumour necrosis factor receptor p55 (sTNF-R55) and sTNF-R75, and of C reactive protein (CRP) and lipopolysaccharide binding protein (LBP) were analysed in 55 patients with stable COPD (median forced expiratory volume in one second (FEV(1)) 34% predicted (range 15-78)) and compared with levels in 23 control subjects. In addition, changes in these mediators were studied in 13 patients with COPD (median FEV(1) 34% predicted (range 19-51)) during the first 7 days in hospital with an exacerbation of the disease., Results: Patients with stable COPD were characterised by a systemic inflammatory process indicated by an increased leucocyte count (7.2 (4.7-16.4) v 4.8 (3.5-8.3) x 10(9)/l), raised levels of CRP (11.8 (1.1-75.0) v 4.1 (0.6-75.0) microg/ml) and LBP (45.6 (8.1-200.0) v 27.9 (14.1-71.5) microg/ml), and moderate increases in both sTNF-Rs. In contrast, the sIL-1RII level did not differ between patients and controls (4.53 (2.09-7.60) v 4.63 (3.80-5.93) ng/ml). During treatment of disease exacerbations, systemic levels of both CRP (at day 3) and LBP (at day 7) were significantly reduced compared with day 1, whereas sIL-1RII levels increased., Conclusions: These data suggest an imbalance in systemic levels of pro- and anti-inflammatory mediators in patients with stable COPD. The increase in the anti-inflammatory mediator sIL-1RII during treatment of exacerbations may contribute to the clinical improvement.
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- 2001
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41. Intratracheal instillation of lipopolysaccharide in mice induces apoptosis in bronchial epithelial cells: no role for tumor necrosis factor-alpha and infiltrating neutrophils.
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Vernooy JH, Dentener MA, van Suylen RJ, Buurman WA, and Wouters EF
- Subjects
- Animals, Bronchi cytology, In Situ Nick-End Labeling, Instillation, Drug, Lung cytology, Lung drug effects, Lymphotoxin-alpha genetics, Lymphotoxin-alpha metabolism, Male, Mice, Mice, Knockout, Neutrophil Infiltration drug effects, Peroxidase metabolism, Respiratory Mucosa cytology, Trachea, Tumor Necrosis Factor-alpha administration & dosage, Tumor Necrosis Factor-alpha antagonists & inhibitors, Tumor Necrosis Factor-alpha genetics, Apoptosis, Bronchi drug effects, Epithelial Cells drug effects, Lipopolysaccharides administration & dosage, Respiratory Mucosa drug effects
- Abstract
This study investigated apoptosis in lungs after local exposure to lipopolysaccharide (LPS). Mice were instilled intratracheally with 5 microg LPS, which corresponds to the amount acquired by smoking approximately 25 cigarettes, and killed at different time points after exposure. Our data demonstrate that local LPS exposure resulted in apoptosis in lungs from 2 h and peaked at 24 h, as detected by ligation-mediated polymerase chain reaction. Morphologic examination and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end label staining demonstrated apoptosis in bronchial epithelial cells early after intratracheal (IT) LPS challenge, whereas infiltrating neutrophils displayed positive staining at 24 and 72 h after exposure. Apoptosis in lungs clearly preceded pulmonary neutrophil infiltration, confirming that neutrophils did not contribute to pulmonary apoptosis at early time points. Further, using three experimental approaches--namely, anti-tumor necrosis factor (TNF)-alpha treatment, IT TNF-alpha instillation, and TNF/lymphotoxin-alpha knockout mice--we demonstrate that TNF-alpha, which was elevated in lungs at both messenger RNA and protein levels after IT LPS challenge, was no primary mediator in LPS-induced apoptosis at early time points. Thus, local LPS exposure in mice resulted in early apoptosis of bronchial epithelial cells independent of infiltrating neutrophils and TNF-alpha, which suggests that apoptosis of bronchial epithelium may be involved in airway injury during exposure to LPS.
- Published
- 2001
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42. Disturbances in leptin metabolism are related to energy imbalance during acute exacerbations of chronic obstructive pulmonary disease.
- Author
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Creutzberg EC, Wouters EF, Vanderhoven-Augustin IM, Dentener MA, and Schols AM
- Subjects
- Acute Disease, Aged, Blood Glucose metabolism, Body Weight physiology, Female, Homeostasis physiology, Humans, Lung Volume Measurements, Male, Middle Aged, Respiratory Insufficiency physiopathology, Risk Factors, Systemic Inflammatory Response Syndrome physiopathology, Energy Metabolism physiology, Leptin blood, Lung Diseases, Obstructive physiopathology
- Abstract
Previously we reported an impaired energy balance in patients with chronic obstructive pulmonary disease (COPD) during an acute disease exacerbation, but limited data are available on the underlying mechanisms. Experimental and clinical research supports the hypothesis of involvement of the hormone leptin in body weight and energy balance homeostasis. The aim of this study was to investigate the course of the energy balance in relation to leptin and the soluble tumor necrosis factor (TNF) receptors (sTNF-R) 55 and 75, plasma glucose, and serum insulin in patients with severe COPD during the first 7 d of hospitalization for an acute exacerbation (n = 17, 11 men, age mean [SD] 66 [10] yr, FEV(1) 36 [12] %pred). For reference values of the laboratory parameters, blood was collected from 23 (16 men) healthy, elderly subjects. On admission, the dietary intake/resting energy expenditure (REE) ratio was severely depressed (1.28 [0.57]), but gradually restored until Day 7 (1.65 [0. 45], p = 0.005 versus Day 1). Glucose and insulin concentrations were elevated on admission, but on Day 7 only plasma glucose was decreased. The sTNF-Rs were not different from healthy subjects and did not change. Plasma leptin, adjusted for fat mass expressed as percentage of body weight (%FM), was elevated on Day 1 compared with healthy subjects (1.82 [3.85] versus 0.32 [0.72] ng%/ml, p = 0.008), but decreased significantly until Day 7 (1.46 [3.77] ng%/ml, p = 0. 015 versus Day 1). On Day 7, sTNF-R55 was, independently of %FM, correlated with the natural logarithm (LN) of leptin (r = 0.65, p = 0.041) and with plasma glucose (r = 0.81, p = 0.015). In addition, the dietary intake/REE ratio was not only inversely related with LN leptin (-0.74, p = 0.037), but also with sTNF-R55 (r = -0.93, p = 0. 001) on day seven. In conclusion, temporary disturbances in the energy balance were seen during an acute exacerbation of COPD, related to increased leptin concentrations as well as to the systemic inflammatory response. Evidence was found that the elevated leptin concentrations were in turn under control of the systemic inflammatory response, and, presumably, the high-dose systemic glucocorticosteroid treatment.
- Published
- 2000
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43. Strong association of interleukin-6 and lipopolysaccharide-binding protein with severity of adverse reactions after diethylcarbamazine treatment of microfilaremic patients.
- Author
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Haarbrink M, Abadi GK, Buurman WA, Dentener MA, Terhell AJ, and Yazdanbakhsh M
- Subjects
- Adolescent, Adult, Aged, Animals, Antibodies, Helminth blood, Female, Humans, Indonesia, Interleukin-10 blood, Male, Microfilariae, Middle Aged, Acute-Phase Proteins, Brugia malayi, Carrier Proteins blood, Diethylcarbamazine adverse effects, Filariasis drug therapy, Filaricides adverse effects, Interleukin-6 blood, Membrane Glycoproteins
- Abstract
To assess the involvement of inflammatory mediators in the development of adverse reactions in filarial patients undergoing treatment, 29 microfilaremic subjects were treated with diethylcarbamazine (DEC). Before and at serial time points after initiation of treatment, plasma levels of inflammatory mediators and DEC were measured, and adverse reactions were recorded. Patients experienced no or mild, moderate, or severe adverse reactions. Increasing pretreatment microfilarial counts were associated with escalating severity of adverse reactions. Plasma concentrations of DEC were not different among patients suffering from varying degrees of illness. Interleukin (IL)-6, IL-10, lipopolysaccharide-binding protein (LBP), and soluble tumor necrosis factor receptors (sTNF-Rs) increased after treatment. IL-6 and LBP, however, showed the strongest association with adverse reactions. Increasing levels of these molecules were closely correlated with the mounting severity of adverse reactions, which raises the possibility that they play an important role in systemic inflammation that arises after DEC treatment of filarial patients.
- Published
- 2000
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44. Production of the acute-phase protein lipopolysaccharide-binding protein by respiratory type II epithelial cells: implications for local defense to bacterial endotoxins.
- Author
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Dentener MA, Vreugdenhil AC, Hoet PH, Vernooy JH, Nieman FH, Heumann D, Janssen YM, Buurman WA, and Wouters EF
- Subjects
- Animals, Blotting, Western, CHO Cells, Carrier Proteins genetics, Cell Line, Cricetinae, Cytokines pharmacology, Dexamethasone pharmacology, Dose-Response Relationship, Drug, Epithelial Cells drug effects, Humans, Interleukin-1 pharmacology, Interleukin-6 pharmacology, Liver cytology, Liver drug effects, Liver metabolism, Lung cytology, Lung drug effects, Lung metabolism, Mice, Pulmonary Alveoli cytology, Pulmonary Alveoli drug effects, Pulmonary Alveoli metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Respiratory Mucosa cytology, Respiratory Mucosa drug effects, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha pharmacology, Acute-Phase Proteins metabolism, Carrier Proteins metabolism, Epithelial Cells metabolism, Membrane Glycoproteins, Respiratory Mucosa metabolism
- Abstract
This study demonstrates for the first time that respiratory epithelial cells are able to produce the acute phase protein lipopolysaccharide (LPS)-binding protein (LBP), which is known to play a central role in the defense to bacterial endotoxins (or LPS). Indications for local presence of LBP in human lung was obtained via reverse transcriptase/polymerase chain reaction that showed LBP messenger RNA (mRNA) expression. Therefore, LBP production by the human lung epithelial cell line A549, a human adenocarcinoma with features of type II pneumocytes, was studied. These cells produced LBP in response to interleukin (IL)-1beta, IL-6, and tumor necrosis factor- alpha, a response that was strongly enhanced by dexamethasone. In addition, LBP mRNA was detected in A549 cells, in increasing amounts as a result of stimulation. The pattern of cytokine-induced LBP production in A549 cells was similar to the pattern in the human liver epithelial cell line HuH-7. Moreover, the molecular weight of A549-derived LBP was approximately 60 kD, which is similar to HuH-7-derived LBP. Biologic activity of LBP produced by A549 cells was evaluated on the basis of its ability to interact with LPS. Further indications that type II alveolar epithelial cells are able to produce LBP were obtained from the observations that the murine lung type II epithelial cell line C10 produced murine LBP, and that isolated human primary type II pneumocytes expressed LBP mRNA, which was enhanced after stimulation of cells. The local production of this endotoxin binding protein by lung epithelial cells might contribute to a highly specific response at the site of exposure to bacteria and bacterial endotoxins.
- Published
- 2000
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45. Release of endotoxin-binding proteins during major elective surgery: role of soluble CD14 in phagocytic activation.
- Author
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Hiki N, Berger D, Mimura Y, Frick J, Dentener MA, Buurman WA, Seidelmann M, Kaminishi M, and Beger HG
- Subjects
- Adult, Aged, Antimicrobial Cationic Peptides, Colectomy, Female, Gastrectomy, Humans, Leukocytes, Mononuclear metabolism, Lipopolysaccharide Receptors blood, Male, Membrane Proteins blood, Middle Aged, Pancreatectomy, Prospective Studies, Acute-Phase Proteins biosynthesis, Blood Proteins biosynthesis, Carrier Proteins biosynthesis, Elective Surgical Procedures, Lipopolysaccharide Receptors biosynthesis, Lipopolysaccharide Receptors immunology, Membrane Glycoproteins, Membrane Proteins biosynthesis, Phagocytes immunology
- Abstract
Our previous study demonstrated that soluble CD14 (sCD14) modulates the biologic activity of circulating endotoxin, which appears after surgery. In this study, we examined the behavior of endotoxin-binding proteins, such as sCD14, lipopolysaccharide-binding protein (LBP), and bactericidal/permeability-increasing protein (BPI), in patients' plasma after major abdominal surgery and the phagocytic secretion of sCD14 from peripheral blood mononuclear cells (PBMCs) throughout the observation period. In a prospective study, 15 patients undergoing major abdominal surgery (gastrectomy, n = 3; pancreatectomy, n = 10: colectomy, n = 2) were involved in this study. The endotoxin-binding proteins were perioperatively (preoperatively; postoperative hour 6; days 1, 2, 3, 4, 5, 7, and 10) measured by an enzyme-linked immunosorbent assay (ELISA). To exclude the hemodilution effect of samples, each parameter was corrected by dividing the respective value by the albumin concentration. The phagocytic activity at each time point was tested as an ex vivo sCD14 secretion from PBMCs in the presence and absence of exogenously added endotoxin, Escherichia coli 055B5 (1 ng/ml). Significant endotoxemia (0.35 +/- 0.13 EU/ml; p < 0.05) was observed 6 hours after the beginning of surgery. The sCD14/albumin value rapidly increased at 6 hours after surgery, peaked on day 1, and sequentially declined, whereas the BPI/albumin and LBP/albumin ratios increased more gradually and peaked on day 2. The secretion of sCD14 from 2 x 10(6) PBMCs was significantly enhanced from 6 hours after operation. The increased plasma level of sCD14 may be explained by the parallel-enhanced sCD14 PBMC production. Activated secretion of these endotoxin-binding proteins may play a role in regulating the biologic activity of circulating endotoxin.
- Published
- 2000
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46. Changes in endotoxin-binding proteins during major elective surgery: important role for soluble CD14 in regulation of biological activity of systemic endotoxin.
- Author
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Hiki N, Berger D, Dentener MA, Mimura Y, Buurman WA, Prigl C, Seidelmann M, Tsuji E, Kaminishi M, and Beger HG
- Subjects
- Adult, Aged, Anti-Bacterial Agents, Antimicrobial Cationic Peptides, Apolipoproteins A blood, Apolipoproteins B blood, Elective Surgical Procedures, Female, Humans, Kinetics, Limulus Test, Lipopolysaccharides analysis, Lipopolysaccharides blood, Lipopolysaccharides immunology, Male, Middle Aged, Monocytes chemistry, Monocytes immunology, Polymyxin B, Postoperative Period, Serum Albumin, Tumor Necrosis Factor-alpha analysis, Tumor Necrosis Factor-alpha immunology, Abdomen surgery, Acute-Phase Proteins, Blood Proteins metabolism, Carrier Proteins blood, Lipopolysaccharide Receptors blood, Lipopolysaccharide Receptors immunology, Membrane Glycoproteins, Membrane Proteins
- Abstract
Assessment of circulating endotoxin during the perioperative period, which is only demonstrated by the Limulus amebocyte lysate (LAL) test, may be modulated by several endotoxin-binding proteins. Endotoxin-neutralizing capacity (ENC) and the plasma levels of soluble CD14 (sCD14), lipopolysaccharide-binding protein, and bactericidal/permeability-increasing protein (BPI) were determined in 40 patients 6 h prior to skin incision for major abdominal surgery. The bioactivity of plasma endotoxin was tested by the polymyxin B-inhibited stimulatory activity of the plasma samples on healthy monocytes as measured by the release of tumor necrosis factor alpha. Plasma endotoxin levels in almost all patients increased from 0.05 +/- 0.01 to 0.23 +/- 0.03 experimental units (EU) per ml (P < 0.001); more specifically, 17 of 40 samples showed endotoxin levels of greater than 0.2 EU per ml and corresponding reductions in ENC. Soluble CD14 plasma levels were decreased from 5. 6 +/- 0.3 to 4.6 +/- 0.3 microg per ml (P < 0.05). ENC was strongly correlated with the sCD14 plasma concentration throughout the period of observation. The addition of sCD14-neutralizing monoclonal anti-sCD14 antibodies reduced ENC both pre- and postoperatively. No correlation could be established between ENC and the plasma levels of BPI, high-density lipoproteins, or low-density lipoproteins determined by measuring the concentrations of apoprotein A and apoprotein B. Biologically active endotoxin was found in only 6 of 17 samples with endotoxin levels greater than 0.2 EU per ml in the LAL test. These samples could be characterized by their perioperative loss of at least 35% of their sCD14. No change in sCD14 was detected in the remaining 11 samples. The perioperative loss of ENC is partly caused by the loss of sCD14 resulting from its consumption by endotoxin reaching the bloodstream. This study demonstrated the role of sCD14 on the bioactivity of circulating endotoxin in a human model of endotoxemia after major abdominal surgery.
- Published
- 1999
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47. Lipopolysaccharide binding protein and serum amyloid A secretion by human intestinal epithelial cells during the acute phase response.
- Author
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Vreugdenhil AC, Dentener MA, Snoek AM, Greve JW, and Buurman WA
- Subjects
- Acute-Phase Reaction immunology, Adjuvants, Immunologic pharmacology, Apolipoproteins biosynthesis, Apolipoproteins immunology, Caco-2 Cells drug effects, Caco-2 Cells immunology, Caco-2 Cells metabolism, Carcinoma, Hepatocellular metabolism, Carrier Proteins biosynthesis, Carrier Proteins chemistry, Carrier Proteins immunology, Cell Line, Cytokines pharmacology, Dexamethasone pharmacology, Endotoxins pharmacology, Escherichia coli immunology, Humans, Ileum metabolism, Intestinal Mucosa drug effects, Intestinal Mucosa immunology, Jejunum metabolism, Kinetics, Serum Amyloid A Protein biosynthesis, Serum Amyloid A Protein immunology, Time Factors, Acute-Phase Proteins, Acute-Phase Reaction metabolism, Apolipoproteins metabolism, Carrier Proteins metabolism, Intestinal Mucosa metabolism, Lipopolysaccharides metabolism, Membrane Glycoproteins, Serum Amyloid A Protein metabolism
- Abstract
The acute phase proteins LPS binding protein (LBP) and serum amyloid A (SAA) are produced by the liver and are present in the circulation. Both proteins have been shown to participate in the immune response to endotoxins. The intestinal mucosa forms a large surface that is continuously exposed to these microbial products. By secretion of antimicrobial and immunomodulating agents, the intestinal epithelium contributes to the defense against bacteria and their products. The aim of this study was to explore the influence of the inflammatory mediators TNF-alpha, IL-6, and IL-1beta on the release of LBP and SAA by intestinal epithelial cells (IEC). In addition, the induction of LBP and SAA release by cell lines of intestinal epithelial cells and hepatic cells was compared. The data obtained show that in addition to liver cells, IEC also expressed LBP mRNA and released bioactive LBP and SAA upon stimulation. Regulation of LBP and SAA release by IEC and hepatocytes was typical for class 1 acute phase proteins, although differences in regulation between the cell types were observed. Endotoxin did not induce LBP and SAA release. Glucocorticoids were demonstrated to strongly enhance the cytokine-induced release of LBP and SAA by IEC, corresponding to hepatocytes. The data from this study, which imply that human IEC can produce LBP and SAA, suggest a role for these proteins in the local defense mechanism of the gut to endotoxin. Furthermore, the results demonstrate that tissues other than the liver are involved in the acute phase response.
- Published
- 1999
48. Prediction of clinical severity and outcome of ventilator-associated pneumonia. Comparison of simplified acute physiology score with systemic inflammatory mediators.
- Author
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Froon AH, Bonten MJ, Gaillard CA, Greve JW, Dentener MA, de Leeuw PW, Drent M, Stobberingh EE, and Buurman WA
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Antimicrobial Cationic Peptides, Bacteria isolation & purification, Blood Bactericidal Activity, Blood Proteins analysis, Bronchoscopy, Case-Control Studies, Cause of Death, Cohort Studies, E-Selectin blood, Forecasting, Humans, Intercellular Adhesion Molecule-1 blood, Middle Aged, Pneumococcal Infections blood, Pneumonia blood, Pneumonia etiology, Pneumonia microbiology, Pseudomonas Infections blood, Pseudomonas aeruginosa, Sepsis microbiology, Shock, Septic microbiology, Streptococcal Infections blood, Survival Rate, Treatment Outcome, APACHE, Inflammation Mediators blood, Membrane Proteins, Pneumonia physiopathology, Ventilators, Mechanical adverse effects
- Abstract
Systemic kinetics of three inflammatory mediators (bactericidal/permeability-increasing protein [BPI], soluble intercellular adhesion molecule [sICAM], and soluble E-selectin [sE-selectin]) were studied during the development of ventilator-associated pneumonia (VAP) (n = 42), diagnosed on quantitative cultures of bronchoscopic samples. From a pool of collected samples, nested samples were used to measure mediators on Days -4, -2, 0, and +2, relative to diagnosis. Correlations between systemic levels of mediators and clinical severity of infection (VAP with or without severe sepsis or septic shock) and patient outcome (mortality at Day 10 after diagnosis) were studied. Predictive values of inflammatory mediators were compared with daily Simplified Acute Physiology Score II (SAPS II) values and the logarithmic number of bacteria in bronchoscopic samples. During the development of VAP, increasing SAPS II scores and rising systemic mediator levels were only found in patients in whom VAP was accompanied with severe sepsis or septic shock. Values of SAPS II and plasma levels of BPI and sE-selectin, but not sICAM, increased from the day of diagnosis on in patients who died within 10 d of diagnosis. Systemic levels of inflammatory mediators did not better predict clinical severity or patient outcome than daily SAPS II scores. The logarithmic number of bacteria in bronchoscopic samples poorly correlated with circulating levels of inflammatory mediators, severity of infection, and patient outcome. Our findings show that a clinical scoring system (SAPS II score) is at least as good as a predictor for the clinical severity of infection and patient outcome, and provide new information on the kinetics of inflammatory mediators during the development of VAP.
- Published
- 1998
- Full Text
- View/download PDF
49. The bactericidal/permeability-increasing protein (BPI) is present in specific granules of human eosinophils.
- Author
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Calafat J, Janssen H, Tool A, Dentener MA, Knol EF, Rosenberg HF, and Egesten A
- Subjects
- Antimicrobial Cationic Peptides, Blood Bactericidal Activity, Blotting, Western, Humans, Microscopy, Electron, Blood Proteins metabolism, Cytoplasmic Granules metabolism, Eosinophils metabolism, Eosinophils ultrastructure, Membrane Proteins
- Abstract
Eosinophils participate in the inflammatory response seen in allergy and parasitic infestation, but a role in host defense against bacterial infection is not settled. The bactericidal/permeability-increasing protein (BPI) has been demonstrated in neutrophils and it exerts bacteriostatic and bactericidal effects against a wide variety of Gram-negative bacterial species. Using the Western blot technique, a 55-kD band, corresponding to BPI, was detected in lysates from both neutrophils and eosinophils. The localization of BPI in immature and mature eosinophils was investigated using immunoelectron microscopy. BPI was found in immature and mature specific granules of eosinophils and was detected in phagosomes as well, indicating release of the protein from the granules into the phagosomes. Using a specific enzyme-linked immunosorbent assay, eosinophils were shown to contain 179 ng of BPI/5 x 10(6) eosinophils compared with 710 ng BPI/5 x 10(6) neutrophils. The presence of BPI in eosinophils suggests a role for these cells in host defense against Gram-negative bacterial invasion or may suggest a role for BPI against parasitic infestation.
- Published
- 1998
50. The enhanced inflammatory response in non-small cell lung carcinoma is not reflected in the alveolar compartment.
- Author
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Staal-van den Brekel AJ, Dentener MA, Drent M, ten Velde GP, Buurman WA, and Wouters EF
- Subjects
- Analysis of Variance, Cells, Cultured, Cytokines analysis, E-Selectin analysis, Female, Humans, Intercellular Adhesion Molecule-1 analysis, Interleukin-6 analysis, Interleukin-8 analysis, Macrophages immunology, Male, Middle Aged, Receptors, Tumor Necrosis Factor analysis, Statistics, Nonparametric, Tumor Necrosis Factor-alpha analysis, Bronchoalveolar Lavage Fluid immunology, Carcinoma, Non-Small-Cell Lung immunology, Inflammation Mediators analysis, Lung Neoplasms immunology
- Abstract
An inflammatory response has been observed in lung cancer both locally and systemically. The aim of the present study was to investigate whether the alveolar compartment was involved in the inflammatory response in non-small cell lung carcinoma (NSCLC). Both inflammatory mediators in bronchoalveolar lavage fluid (BALF) and cytokines produced by alveolar macrophages (AM) were investigated. Twenty patients with newly detected NSCLC and nine control subjects were studied. The patients had not been treated with chemotherapy, radiotherapy or with systemic or inhaled corticosteroids. All patients and control subjects were current smokers or stopped smoking recently. BAL was performed in the affected lung as well as in the contralateral lung of NSCLC patients, and only unilaterally in control subjects. Comparable results were demonstrated for the levels of the of the inflammatory mediators TNF-a, Interleukin (IL)-6, IL-8, both soluble TNF receptors and the soluble adhesion molecules E-selectin and intercellular adhesion molecule (ICAM)-1 between the affected lung and the contralateral lung in the NSCLC population as well as between the NSCLC population and the control subjects. Moreover, no significant differences in cytokine profiles of AM were found between AM obtained from the affected lung and from the contralateral lung. Although BAL is a useful tool in the diagnostic procedure for NSCLC, the present findings suggest that BAL does not reflect the enhanced inflammatory state, as reported in plasma and in the interstitial compartment around the tumour cells in NSCLC.
- Published
- 1998
- Full Text
- View/download PDF
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