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238 results on '"Deniset-Besseau, Ariane"'

1. Multiscale approach to provide a better physicochemical description of women breast microcalcifications

Author

Petay, Margaux, Cherfan, Maguy, Bouderlique, Elise, Reguer, Solenn, Mathurin, Jérémie, Dazzi, Alexandre, L’Heronde, Maëva, Daudon, Michel, Letavernier, Emmanuel, Deniset-Besseau, Ariane, and Bazin, Dominique

Subjects
Breast cancer, Microcalcification, Scanning electron microscopy, Infrared microspectroscopy, Infrared nanospectroscopy, AFM-IR, Biochemistry, QD415-436, Physical and theoretical chemistry, QD450-801, Mathematics, QA1-939
Abstract

Despite the incidence of breast cancer among women, mammography and anatomopathology investigations are still the gold standard method for preventive screening and diagnosis. Several criteria are used to diagnose precisely the severity of the pathology like the distribution and shape of breast microcalcifications (BMCs). However, the link between the different chemical phases of BMCs and the cancer stage remains unclear. As BMCs physicochemical speciation has the potential to help clinicians during their diagnosis, this study aims to propose a methodology using advanced spectroscopical analysis techniques to finely characterize BMCs and uncover the relationship between mineralization processes and breast cancer. A state of the art in the domain is first proposed to highlight the role of BMCs and the importance of extensive analytical analysis using electron microscopy and vibrational techniques. Secondly, a detailed methodology for BMCs multiscale analysis is proposed and the relevance of each technique illustrated through the study of a biopsy from a patient suffering of an infiltrating low-grade ductal carcinoma: scanning electron microscopy analysis was used for the morphological description of BMCs, infrared micro and nanospectroscopy techniques for their chemical speciation at the micrometric and sub-micrometric scales.

Published
2022
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2. Profile of an 'at cutting edge' pathology laboratory for pathological human deposits: from nanometer to in vivo scale analysis on large scale facilities

Author

Bazin, Dominique, Lucas, Ivan T., Rouzière, Stéphan, Elkaim, Erik, Mocuta, Cristian, Réguer, Solenn, Reid, David G., Mathurin, Jérémie, Dazzi, Alexandre, Deniset-Besseau, Ariane, Petay, Margaux, Frochot, Vincent, Haymann, Jean-Philippe, Letavernier, Emmanuel, Verpont, Marie-Christine, Foy, Eddy, Bouderlique, Elise, Colboc, Hester, and Daudon, Michel

Subjects
Pathological calcifications, Characterization techniques, Experimental approach, Medical diagnostic, Abnormal deposits, Biochemistry, QD415-436, Physical and theoretical chemistry, QD450-801, Mathematics, QA1-939
Abstract

This contribution aims to define an analysis procedure for abnormal deposits in human tissues starting from in vivo characterization, down to the nanoscale using major instrumentation. Such an integrated approach is based on recent literature, but particularly on our research over the last twenty years on pathological calcifications. To this end, we begin by describing four successive analytical steps, on the injury site or physician’s surgery, at the hospital, at a typical physicochemical laboratory, and finally at a large scale (possibly multinational) facility. For the first step, we present various techniques which can be implemented on portable instruments. For the second step, commercial analytical setups are used. In a physicochemical laboratory, prototype or commercial setups are used and finally on large scale instruments, characterization techniques with better spatial resolution and/or higher sensitivity or techniques specific to synchrotron radiation are employed.

Published
2022
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3. Cystinuria and cystinosis are usually related to L-cystine: is this really the case for cystinosis? A physicochemical investigation at micrometre and nanometre scale

Author

Bazin, Dominique, Rabant, Marion, Mathurin, Jérémie, Petay, Margaux, Deniset-Besseau, Ariane, Dazzi, Alexandre, Su, Yangyang, Hessou, Etienne P., Tielens, Frederik, Borondics, Ferenc, Livrozet, Marine, Bouderlique, Elise, Haymann, Jean-Philippe, Letavernier, Emmanuel, Frochot, Vincent, and Daudon, Michel

Subjects
Cystinuria, Cystinosis, FTIR, SEM, AFM-IR, OPTIR, Biochemistry, QD415-436, Physical and theoretical chemistry, QD450-801, Mathematics, QA1-939
Abstract

Medical literature indicates clearly that cystinuria and cystinosis, two severe genetic pathologies, are related to the presence of abnormal L-cystine deposits. While L-cystine adopts a hexagonal crystal morphology consistent with its crystallographic structure (hexagonal, $\mathrm{P}6_{1}22$ space group), abnormal deposits related to cystinosis display a rectangular shape. Because this is unexpected from the hexagonal crystallographic structure of L-cystine, we have investigated this inconsistency using SEM (scanning electron microscopy) and IR (infrared) spectroscopy at micrometre and nanometre scales. Our data clearly indicate the presence of both L-cysteine and L-cystine. Considering that L-cysteine crystals display a rectangular shape, and that a transition phase between L-cysteine and L-cystine is well known, we propose the following model for deposit evolution in cystinosis. The initial abnormal deposit consists of L-cysteine, with a rectangular crystal morphology. The micrometre scale rectangular crystallite shape is retained after the phase transition equilibrium between L-cysteine and L-cystine is established, with some crystalline L-cysteine still remaining.

Published
2021
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4. Microscale Hydrogen, Carbon, and Nitrogen Isotopic Diversity of Organic Matter in Asteroid Ryugu

Author

Nittler, Larry R, Barosch, Jens, Burgess, Katherine, Stroud, Rhonda M, Wang, Jianhua, Yabuta, Hikaru, Enokido, Yuma, Matsumoto, Megumi, Nakamura, Tomoki, Kebukawa, Yoko, Yamashita, Shohei, Takahashi, Yoshio, Bejach, Laure, Bonal, Lydie, Cody, George D, Dartois, Emmanuel, Dazzi, Alexandre, De Gregorio, Bradley, Deniset-Besseau, Ariane, Duprat, Jean, Engrand, Cécile, Hashiguchi, Minako, Kilcoyne, A. L. David, Komatsu, Mutsumi, Martins, Zita, Mathurin, Jérémie, Montagnac, Gilles, Mostefaoui, Smail, Okumura, Taiga, Quirico, Eric, Remusat, Laurent, Sandford, Scott, Shigenaka, Miho, Suga, Hiroki, Takeichi, Yasuo, Tamenori, Yusuke, Verdier-Paoletti, Maximilien, Wakabayashi, Daisuke, Abe, Masanao, Kamide, Kanami, Miyazaki, Akiko, Nakato, Aiko, Nakazawa, Satoru, Nishimura, Masahiro, Okada, Tatsuaki, Saiki, Takanao, Tanaka, Satoshi, Terui, Fuyuto, Usui, Tomohiro, Yada, Toru, Yogata, Kasumi, Yoshikawa, Makoto, Yurimoto, Hisayoshi, Noguchi, Takaaki, Okazaki, Ryuji, Naraoka, Hiroshi, Sakamoto, Kanako, Tachibana, Shogo, Watanabe, Sei-ichiro, and Tsuda, Yuichi

Subjects
Astrophysics - Earth and Planetary Astrophysics
Abstract

We report the H, C, and N isotopic compositions of microscale (0.2 to 2$\mu$m) organic matter in samples of asteroid Ryugu and the Orgueil CI carbonaceous chondrite. Three regolith particles of asteroid Ryugu, returned by the Hayabusa2 spacecraft, and several fragments of Orgueil were analyzed by NanoSIMS isotopic imaging. The isotopic distributions of the Ryugu samples from two different collection spots are closely similar to each other and to the Orgueil samples, strengthening the proposed Ryugu-CI chondrite connection. Most individual sub-$\mu$m organic grains have isotopic compositions within error of bulk values, but 2-8% of them are outliers exhibiting large isotopic enrichments or depletions in D, $^{15}$N, and/or $^{13}$C. The H, C and N isotopic compositions of the outliers are not correlated with each other: while some C-rich grains are both D- and $^{15}$N-enriched, many are enriched or depleted in one or the other system. This most likely points to a diversity in isotopic fractionation pathways and thus diversity in the local formation environments for the individual outlier grains. The observation of a relatively small population of isotopic outlier grains can be explained either by escape from nebular and/or parent body homogenization of carbonaceous precursor material or addition of later isotopic outlier grains. The strong chemical similarity of isotopically typical and isotopically outlying grains, as reflected by synchrotron x-ray absorption spectra, suggests a genetic connection and thus favors the former, homogenization scenario. However, the fact that even the least altered meteorites show the same pattern of a small population of outliers on top of a larger population of homogenized grains indicates that some or most of the homogenization occurred prior to accretion of the macromolecular organic grains into asteroidal parent bodies., Comment: Accepted for publication in Earth and Planetary Science Letters. 8 figures (plus 3 supplementary figs), two tables

Published
2024

6. Variations of organic functional chemistry in carbonaceous matter from the asteroid 162173 Ryugu

Author

De Gregorio, Bradley, Cody, George D., Stroud, Rhonda M., David Kilcoyne, A. L., Sandford, Scott, Le Guillou, Corentin, Nittler, Larry R., Barosch, Jens, Yabuta, Hikaru, Martins, Zita, Kebukawa, Yoko, Okumura, Taiga, Hashiguchi, Minako, Yamashita, Shohei, Takeichi, Yasuo, Takahashi, Yoshio, Wakabayashi, Daisuke, Engrand, Cécile, Bejach, Laure, Bonal, Lydie, Quirico, Eric, Remusat, Laurent, Duprat, Jean, Verdier-Paoletti, Maximilien, Mostefaoui, Smail, Komatsu, Mutsumi, Mathurin, Jérémie, Dazzi, Alexandre, Deniset-Besseau, Ariane, Dartois, Emmanuel, Tamenori, Yusuke, Suga, Hiroki, Montagnac, Gilles, Kamide, Kanami, Shigenaka, Miho, Matsumoto, Megumi, Enokido, Yuma, Yoshikawa, Makoto, Saiki, Takanao, Tanaka, Satoshi, Terui, Fuyuto, Nakazawa, Satoru, Usui, Tomohiro, Abe, Masanao, Okada, Tatsuaki, Yada, Toru, Nishimura, Masahiro, Nakato, Aiko, Miyazaki, Akiko, Yogata, Kasumi, Yurimoto, Hisayoshi, Nakamura, Tomoki, Noguchi, Takaaki, Okazaki, Ryuji, Naraoka, Hiroshi, Sakamoto, Kanako, Tachibana, Shogo, Watanabe, Sei-ichiro, and Tsuda, Yuichi

Published
2024
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7. Presolar stardust in asteroid Ryugu

Author

Barosch, Jens, Nittler, Larry R., Wang, Jianhua, Alexander, Conel M. O'D., De Gregorio, Bradley T., Engrand, Cécile, Kebukawa, Yoko, Nagashima, Kazuhide, Stroud, Rhonda M., Yabuta, Hikaru, Abe, Yoshinari, Aléon, Jérôme, Amari, Sachiko, Amelin, Yuri, Bajo, Ken-ichi, Bejach, Laure, Bizzarro, Martin, Bonal, Lydie, Bouvier, Audrey, Carlson, Richard W., Chaussidon, Marc, Choi, Byeon-Gak, Cody, George D., Dartois, Emmanuel, Dauphas, Nicolas, Davis, Andrew M., Dazzi, Alexandre, Deniset-Besseau, Ariane, Di Rocco, Tommaso, Duprat, Jean, Fujiya, Wataru, Fukai, Ryota, Gautam, Ikshu, Haba, Makiko K., Hashiguchi, Minako, Hibiya, Yuki, Hidaka, Hiroshi, Homma, Hisashi, Hoppe, Peter, Huss, Gary R., Ichida, Kiyohiro, Iizuka, Tsuyoshi, Ireland, Trevor R., Ishikawa, Akira, Ito, Motoo, Itoh, Shoichi, Kamide, Kanami, Kawasaki, Noriyuki, Kilcoyne, A. L. David, Kita, Noriko T., Kitajima, Kouki, Kleine, Thorsten, Komatani, Shintaro, Komatsu, Mutsumi, Krot, Alexander N., Liu, Ming-Chang, Martins, Zita, Masuda, Yuki, Mathurin, Jérémie, McKeegan, Kevin D., Montagnac, Gilles, Morita, Mayu, Mostefaoui, Smail, Motomura, Kazuko, Moynier, Frédéric, Nakai, Izumi, Nguyen, Ann N., Ohigashi, Takuji, Okumura, Taiga, Onose, Morihiko, Pack, Andreas, Park, Changkun, Piani, Laurette, Qin, Liping, Quirico, Eric, Remusat, Laurent, Russell, Sara S., Sakamoto, Naoya, Sandford, Scott A., Schönbächler, Maria, Shigenaka, Miho, Suga, Hiroki, Tafla, Lauren, Takahashi, Yoshio, Takeichi, Yasuo, Tamenori, Yusuke, Tang, Haolan, Terada, Kentaro, Terada, Yasuko, Usui, Tomohiro, Verdier-Paoletti, Maximilien, Wada, Sohei, Wadhwa, Meenakshi, Wakabayashi, Daisuke, Walker, Richard J., Yamashita, Katsuyuki, Yamashita, Shohei, Yin, Qing-Zhu, Yokoyama, Tetsuya, Yoneda, Shigekazu, Young, Edward D., Yui, Hiroharu, Zhang, Ai-Cheng, Abe, Masanao, Miyazaki, Akiko, Nakato, Aiko, Nakazawa, Satoru, Nishimura, Masahiro, Okada, Tatsuaki, Saiki, Takanao, Tanaka, Satoshi, Terui, Fuyuto, Tsuda, Yuichi, Watanabe, Sei-ichiro, Yada, Toru, Yogata, Kasumi, Yoshikawa, Makoto, Nakamura, Tomoki, Naraoka, Hiroshi, Noguchi, Takaaki, Okazaki, Ryuji, Sakamoto, Kanako, Tachibana, Shogo, and Yurimoto, Hisayoshi

Subjects
Astrophysics - Earth and Planetary Astrophysics, Astrophysics - Solar and Stellar Astrophysics
Abstract

We have conducted a NanoSIMS-based search for presolar material in samples recently returned from C-type asteroid Ryugu as part of JAXA's Hayabusa2 mission. We report the detection of all major presolar grain types with O- and C-anomalous isotopic compositions typically identified in carbonaceous chondrite meteorites: 1 silicate, 1 oxide, 1 O-anomalous supernova grain of ambiguous phase, 38 SiC, and 16 carbonaceous grains. At least two of the carbonaceous grains are presolar graphites, whereas several grains with moderate C isotopic anomalies are probably organics. The presolar silicate was located in a clast with a less altered lithology than the typical extensively aqueously altered Ryugu matrix. The matrix-normalized presolar grain abundances in Ryugu are 4.8$^{+4.7}_{-2.6}$ ppm for O-anomalous grains, 25$^{+6}_{-5}$ ppm for SiC grains and 11$^{+5}_{-3}$ ppm for carbonaceous grains. Ryugu is isotopically and petrologically similar to carbonaceous Ivuna-type (CI) chondrites. To compare the in situ presolar grain abundances of Ryugu with CI chondrites, we also mapped Ivuna and Orgueil samples and found a total of SiC grains and 6 carbonaceous grains. No O-anomalous grains were detected. The matrix-normalized presolar grain abundances in the CI chondrites are similar to those in Ryugu: 23 $^{+7}_{-6}$ ppm SiC and 9.0$^{+5.3}_{-4.6}$ ppm carbonaceous grains. Thus, our results provide further evidence in support of the Ryugu-CI connection. They also reveal intriguing hints of small-scale heterogeneities in the Ryugu samples, such as locally distinct degrees of alteration that allowed the preservation of delicate presolar material., Comment: 12 pages, 3 figures, 2 tables. Published in ApJL

Published
2022
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8. The thermal history of Ryugu based on Raman characterization of Hayabusa2 samples

Author

Bonal, Lydie, Quirico, Eric, Montagnac, Gilles, Komatsu, Mutsumi, Kebukawa, Yoko, Yabuta, Hikaru, Amano, Kana, Barosch, Jens, Bejach, Laure, Cody, George D., Dartois, Emmanuel, Dazzi, Alexandre, De Gregorio, Bradley, Deniset-Besseau, Ariane, Duprat, Jean, Engrand, Cécile, Hashiguchi, Minako, Kamide, Kanami, Kilcoyne, David, Martins, Zita, Mathurin, Jérémie, Mostefaoui, Smail, Nittler, Larry, Ohigashi, Takuji, Okumura, Taiga, Remusat, Laurent, Sandford, Scott, Shigenaka, Miho, Stroud, Rhonda, Suga, Hiroki, Takahashi, Yoshio, Takeichi, Yasuo, Tamenori, Yusuke, Verdier-Paoletti, Maximilien, Yamashita, Shohei, Nakamura, Tomoki, Naraoka, Hiroshi, Noguchi, Takaaki, Okazaki, Ryuji, Yurimoto, Hisayoshi, Tachibana, Shogo, Abe, Masanao, Miyazaki, Akiko, Nakato, Aiko, Nakazawa, Satoru, Nishimura, Masahiro, Okada, Tatsuaki, Saiki, Takanao, Sakamoto, Kanako, Tanaka, Satoshi, Terui, Fuyuto, Tsuda, Yuichi, Usui, Tomohiro, Watanabe, Sei-ichiro, Yada, Toru, Yogata, Kasumi, and Yoshikawa, Makota

Published
2024
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10. Complex mixture of organic matter in a xenolithic clast from the Zag meteorite revealed by coordinated analyses using AFM-IR, NanoSIMS and STXM/XANES

Author

Kebukawa, Yoko, Mathurin, Jérémie, Dartois, Emmanuel, Dazzi, Alexandre, Deniset-Besseau, Ariane, Duprat, Jean, Remusat, Laurent, Noguchi, Takaaki, Miyake, Akira, Igami, Yohei, Paoletti, Maximilien Verdier, Zolensky, Michael E., Engrand, Cécile, Sandt, Christophe, Borondics, Ferenc, Yamashita, Shohei, Wakabayashi, Daisuke, Takeichi, Yasuo, and Takahashi, Yoshio

Published
2023
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14. AFM-IR nanospectroscopy of nanoglobule-like particles in Ryugu samples returned by the Hayabusa2 mission

Author

Mathurin, Jérémie, primary, Bejach, Laure, additional, Dartois, Emmanuel, additional, Engrand, Cécile, additional, Dazzi, Alexandre, additional, Deniset-Besseau, Ariane, additional, Duprat, Jean, additional, Kebukawa, Yoko, additional, Yabuta, Hikaru, additional, Bonal, Lydie, additional, Quirico, Eric, additional, Sandt, Christophe, additional, Borondics, Ferenc, additional, Barosch, Jens, additional, Beck, Pierre, additional, Cody, George D., additional, De Gregorio, Brad T., additional, Hashiguchi, Minako, additional, Kilcoyne, David A. L., additional, Komatsu, Mutsumi, additional, Martins, Zita, additional, Matsumoto, Megumi, additional, Montagnac, Gilles, additional, Mostefaoui, Smail, additional, Nittler, Larry R., additional, Ohigashi, Takuji, additional, Okumura, Taiga, additional, Phan, Van T. H., additional, Remusat, Laurent, additional, Sandford, Scott, additional, Shigenaka, Miho, additional, Stroud, Rhonda, additional, Suga, Hiroki, additional, Takahashi, Yoshio, additional, Takeichi, Yasuo, additional, Tamenori, Yusuke, additional, Verdier-Paoletti, Maximilien, additional, Yamashita, Shohei, additional, Nakamura, Tomoki, additional, Morita, Tomoyo, additional, Kikuiri, Mizuha, additional, Amano, Kana, additional, Kagawa, Eiichi, additional, Noguchi, Takaaki, additional, Naraoka, Hiroshi, additional, Okazaki, Ryuji, additional, Sakamoto, Kanako, additional, Yurimoto, Hisayoshi, additional, Abe, Masanao, additional, Kamide, Kanami, additional, Miyazaki, Akiko, additional, Nakato, Aiko, additional, Nakazawa, Satoru, additional, Nishimura, Masahiro, additional, Okada, Tatsuaki, additional, Saiki, Takanao, additional, Tachibana, Shogo, additional, Tanaka, Satoshi, additional, Terui, Fuyuto, additional, Tsuda, Yuichi, additional, Usui, Tomohiro, additional, Watanabe, Sei-ichiro, additional, Yada, Toru, additional, Yogata, Kasumi, additional, and Yoshikawa, Makoto, additional

Published
2024
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15. Photothermal AFM-IR Depth Sensitivity: An Original Pathway to Tomographic Reconstruction

Author

Dazzi, Alexandre, Mathurin, Jeremie, Leclere, Philippe, Nickmilder, Pierre, De Wolf, Peter, Wagner, Martin, Hu, Qichi, and Deniset-Besseau, Ariane

Abstract

Photothermal atomic force microscopy-infrared (AFM-IR) enables label-free chemical imaging and spectroscopy with nanometer-scale spatial resolution through the integration of atomic force microscopy (AFM) and infrared radiation. The capability for subsurface and three-dimensional (3D) tomographic material analysis remains, however, largely unexplored. Here, we establish a simple and robust empirical relationship between the probing depth and laser repetition rate for three important modes of AFM-IR operation: resonance-enhanced, tapping, and surface-sensitive AFM-IR. Using this empirical relationship, we demonstrate, based on the example of resonance-enhanced operation, how photothermal AFM-IR of thin surface/subsurface layers of polystyrene domains in the poly(methyl methacrylate) matrix can result in 3D representations revealing the size and thickness of small polystyrene domains in the poly(methyl methacrylate) matrix with nanometer-scale resolution. Experimental findings are confirmed by analytical models.

Published
2024
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16. Raman spectroscopy of Ryugu particles and their extracted residues: Fluorescence background characteristics and similarities to CI chondrites.

Author

Komatsu, Mutsumi, Yabuta, Hikaru, Kebukawa, Yoko, Bonal, Lydie, Quirico, Eric, Fagan, Timothy J., Cody, George D., Barosch, Jens, Bejach, Laure, Dartois, Emmanuel, Dazzi, Alexandre, De Gregorio, Bradley, Deniset‐Besseau, Ariane, Duprat, Jean, Engrand, Cecile, Hashiguchi, Minako, Martins, Zita, Mathurin, Jérémie, Montagnac, Gilles, and Mostefaoui, Smail

Subjects
RAMAN spectroscopy, CHONDRITES, FLUORESCENCE, ORGANIC compounds, GRAPHITE
Abstract

We present here an investigation of Ryugu particles recovered by the Hayabusa2 space mission and their extracted carbonaceous acid residues using Raman spectroscopy. Raman parameters of Ryugu intact grains and their acid residues are characterized by broad D (defect induced) and G (graphite) band widths, indicating the presence of polyaromatic carbonaceous matter with low thermal maturity. Raman spectra of Ryugu particles and CI (type 1) chondrites exhibit stronger laser‐induced fluorescence backgrounds compared to Type 2 and Type 3 carbonaceous chondrites. The high fluorescence signatures and wide bandwidths of the D and G bands of Ryugu intact grains are similar to the Raman spectra observed in CI chondrites, reflecting the low structural order of their aromatic carbonaceous matter, and strengthening the link between Ryugu particles and CI chondrites. The high fluorescence background intensity of the Ryugu particles is due to multiple causes, but it is likely that the relative abundance of geometry‐bearing macromolecular organic matter in total organic carbon contents makes a large contribution to the fluorescence intensities. Locally observed high fluorescence in the acid‐extracted residues of Ryugu is due to nitrogen‐bearing outlier phase. The high fluorescence signature is one consequence of the low degree of thermal maturity of the organic matter and supports evidence that the Ryugu particles have escaped significant parent body thermal metamorphism. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Compositional heterogeneity of insoluble organic matter extracted from asteroid Ryugu samples.

Author

Quirico, Eric, Bonal, Lydie, Kebukawa, Yoko, Amano, Kana, Yabuta, Hikaru, Phan, Van T. H., Beck, Pierre, Rémusat, Laurent, Dartois, Emmanuel, Engrand, Cecile, Martins, Zita, Bejach, Laure, Dazzi, Alexandre, Deniset‐Besseau, Ariane, Duprat, Jean, Mathurin, Jérémie, Montagnac, Gilles, Barosch, Jens, Cody, George D., and De Gregorio, Bradley

Subjects
HYDROTHERMAL alteration, CHONDRITES, FOURIER transforms, FUNCTIONAL analysis, FUNCTIONAL groups
Abstract

We report a Fourier transform infrared analysis of functional groups in insoluble organic matter (IOM) extracted from a series of 100–500 μm Ryugu grains collected during the two touchdowns of February 22 and July 11, 2019. IOM extracted from most of the samples is very similar to IOM in primitive CI, CM, and CR chondrites, and shows that the extent of thermal metamorphism in Ryugu regolith was, at best, very limited. One sample displays chemical signatures consistent with a very mild heating, likely due to asteroidal collision impacts. We also report a lower carbonyl abundance in Ryugu IOM samples compared to primitive chondrites, which could reflect the accretion of a less oxygenated precursor by Ryugu. The possible effects of hydrothermal alteration and terrestrial weathering are also discussed. Last, no firm conclusions could be drawn on the origin of the soluble outlier phases, observed along with IOM in this study and in the preliminary analysis of Ryugu samples. However, it is clear that the HF/HCl residues presented in this publication are a mix between IOM and the nitrogen‐rich outlier phase. [ABSTRACT FROM AUTHOR]

Published
2024
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18. Infrared absorption spectra from organic matter in the asteroid Ryugu samples: Some unique properties compared to unheated carbonaceous chondrites.

Author

Kebukawa, Yoko, Quirico, Eric, Dartois, Emmanuel, Yabuta, Hikaru, Bejach, Laure, Bonal, Lydie, Dazzi, Alexandre, Deniset‐Besseau, Ariane, Duprat, Jean, Engrand, Cecile, Mathurin, Jérémie, Barosch, Jens, Cody, George D., De Gregorio, Bradley, Hashiguchi, Minako, Kamide, Kanami, Kilcoyne, David, Komatsu, Mutsumi, Martins, Zita, and Montagnac, Gilles

Subjects
CARBONACEOUS chondrites (Meteorites), INFRARED absorption, ABSORPTION spectra, INFRARED spectra, METHYLENE group, ASTEROIDS
Abstract

The infrared spectral characteristics of organic‐rich acid residues prepared from Ryugu samples returned by the JAXA Hayabusa2 mission generally match those from unheated carbonaceous chondrite meteorites, but the residues from Ryugu are richer in methyl and methylene functional groups and have higher CH2/CH3 ratios. Moreover, two distinct outlier carbonaceous phases are found; one with spectral characteristics of N‐H functional groups, likely amides, and a second phase containing less nitrogen. Such infrared characteristics of Ryugu organic matter might indicate the pristine nature of the freshly collected samples and reflect the near‐surface chemistry in the parent asteroid. [ABSTRACT FROM AUTHOR]

Published
2024
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20. Compositional heterogeneity of insoluble organic matter extracted from asteroid Ryugu samples

Author

Quirico, Eric, primary, Bonal, Lydie, additional, Kebukawa, Yoko, additional, Amano, Kana, additional, Yabuta, Hikaru, additional, Phan, Van T. H., additional, Beck, Pierre, additional, Rémusat, Laurent, additional, Dartois, Emmanuel, additional, Engrand, Cecile, additional, Martins, Zita, additional, Bejach, Laure, additional, Dazzi, Alexandre, additional, Deniset‐Besseau, Ariane, additional, Duprat, Jean, additional, Mathurin, Jérémie, additional, Montagnac, Gilles, additional, Barosch, Jens, additional, Cody, George D., additional, De Gregorio, Bradley, additional, Enokido, Yuma, additional, Hashiguchi, Minako, additional, Kamide, Kanami, additional, Kilcoyne, David, additional, Komatsu, Mutsumi, additional, Matsumoto, Megumi, additional, Mostefaoui, Smail, additional, Nittler, Larry, additional, Ohigashi, Takuji, additional, Okumura, Taiga, additional, Sandford, Scott, additional, Shigenaka, Miho, additional, Stroud, Rhonda, additional, Suga, Hiroki, additional, Takahashi, Yoshio, additional, Takeichi, Yasuo, additional, Tamenori, Yusuke, additional, Verdier‐Paoletti, Maximilien, additional, Wakabayashi, Daisuke, additional, Yamashita, Shohei, additional, Nakamura, Tomoki, additional, Naraoka, Hiroshi, additional, Noguchi, Takaaki, additional, Okazaki, Ryuji, additional, Yurimoto, Hisayoshi, additional, Sakamoto, Kanako, additional, Tachibana, Shogo, additional, Watanabe, Sei‐Ichiro, additional, Tsuda, Yuichi, additional, Yada, Toru, additional, Nishimura, Masahiro, additional, Nakato, Aiko, additional, Miyazaki, Akiko, additional, Yogata, Kasumi, additional, Abe, Masanao, additional, Okada, Tatsuaki, additional, Usui, Tomohitro, additional, Yoshikawa, Makoto, additional, Saiki, Takanao, additional, Tanaka, Satoshi, additional, Terui, Fuyuto, additional, and Nakazawa, Satoru, additional

Published
2023
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21. Infrared absorption spectra from organic matter in the asteroid Ryugu samples: Some unique properties compared to unheated carbonaceous chondrites

Author

Kebukawa, Yoko, primary, Quirico, Eric, additional, Dartois, Emmanuel, additional, Yabuta, Hikaru, additional, Bejach, Laure, additional, Bonal, Lydie, additional, Dazzi, Alexandre, additional, Deniset‐Besseau, Ariane, additional, Duprat, Jean, additional, Engrand, Cecile, additional, Mathurin, Jérémie, additional, Barosch, Jens, additional, Cody, George D., additional, De Gregorio, Bradley, additional, Hashiguchi, Minako, additional, Kamide, Kanami, additional, Kilcoyne, David, additional, Komatsu, Mutsumi, additional, Martins, Zita, additional, Montagnac, Gilles, additional, Mostefaoui, Smail, additional, Nittler, Larry R., additional, Ohigashi, Takuji, additional, Okumura, Taiga, additional, Remusat, Laurent, additional, Sandford, Scott, additional, Shigenaka, Miho, additional, Stroud, Rhonda, additional, Suga, Hiroki, additional, Takahashi, Yoshio, additional, Takeichi, Yasuo, additional, Tamenori, Yusuke, additional, Verdier‐Paoletti, Maximilien, additional, Wakabayashi, Daisuke, additional, Yamashita, Shohei, additional, Yurimoto, Hisayoshi, additional, Nakamura, Tomoki, additional, Noguchi, Takaaki, additional, Okazaki, Ryuji, additional, Naraoka, Hiroshi, additional, Sakamoto, Kanako, additional, Tachibana, Shogo, additional, Yada, Toru, additional, Nishimura, Masahiro, additional, Nakato, Aiko, additional, Miyazaki, Akiko, additional, Yogata, Kasumi, additional, Abe, Masanao, additional, Okada, Tatsuaki, additional, Usui, Tomohiro, additional, Yoshikawa, Makoto, additional, Saiki, Takanao, additional, Tanaka, Satoshi, additional, Terui, Fuyuto, additional, Nakazawa, Satoru, additional, Watanabe, Sei‐ichiro, additional, and Tsuda, Yuichi, additional

Published
2023
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22. Photothermal AFM-IR spectroscopy and imaging: Status, challenges, and trends.

Author

Mathurin, Jeremie, Deniset-Besseau, Ariane, Bazin, Dominique, Dartois, Emmanuel, Wagner, Martin, and Dazzi, Alexandre

Subjects
*PHOTOTHERMAL spectroscopy, *SPECTRAL imaging, *ATOMIC force microscopy, *CHEMICAL microscopy, *SPATIAL resolution, *INFRARED spectroscopy
Abstract

This article focuses on the atomic force microscopy-infrared (AFM-IR) technique and its recent technological developments. Based on the detection of the photothermal sample expansion signal, AFM-IR combines the high spatial resolution of atomic force microscopy with the chemical identification capability of infrared spectroscopy to achieve submicrometric physico-chemical analyses. Since the first publication in 2005, technological improvements have dramatically advanced the capabilities of AFM-IR in terms of spatial and spectral resolution, sensitivity, and fields of applications. The goal of this paper is to provide an overview of these developments and ongoing limitations. We summarize recent progress in AFM-IR implementations based on the major AFM contact, tapping, and peak force tapping modes. Additionally, three new trends are presented, namely, AFM-IR applied to mineral samples, in fluid and a novel, purely surface sensitive AFM-IR configuration, to probe top layers. These trends demonstrate the immense potential of the technique and offer a good insight into the scope of AFM-IR. [ABSTRACT FROM AUTHOR]

Published
2022
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23. Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics

Author

Dulermo, Thierry, primary, Lejeune, Clara, additional, Aybeke, Ece, additional, Abreu, Sonia, additional, Bleton, Jean, additional, David, Michelle, additional, Deniset-Besseau, Ariane, additional, Chaminade, Pierre, additional, Thibessard, Annabelle, additional, Leblond, Pierre, additional, and Virolle, Marie-Joelle, additional

Published
2023
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25. Chemical composition of carbonaceous asteroid Ryugu from synchrotron spectroscopy in the mid- to far-infrared of Hayabusa2-returned samples

Author

Dartois, Emmanuel, primary, Kebukawa, Yoko, additional, Yabuta, Hikaru, additional, Mathurin, Jérémie, additional, Engrand, Cécile, additional, Duprat, Jean, additional, Bejach, Laure, additional, Dazzi, Alexandre, additional, Deniset-Besseau, Ariane, additional, Bonal, Lydie, additional, Quirico, Eric, additional, Sandt, Christophe, additional, Borondics, Ferenc, additional, Barosch, Jens, additional, Cody, George D., additional, De Gregorio, Brad T., additional, Hashiguchi, Minako, additional, Kilcoyne, David A. L., additional, Komatsu, Mutsumi, additional, Martins, Zita, additional, Matsumoto, Megumi, additional, Montagnac, Gilles, additional, Mostefaoui, Smail, additional, Nittler, Larry R., additional, Ohigashi, Takuji, additional, Okumura, Taiga, additional, Remusat, Laurent, additional, Sandford, Scott, additional, Shigenaka, Miho, additional, Stroud, Rhonda, additional, Suga, Hiroki, additional, Takahashi, Yoshio, additional, Takeichi, Yasuo, additional, Tamenori, Yusuke, additional, Verdier-Paoletti, Maximilien, additional, Yamashita, Shohei, additional, Nakamura, Tomoki, additional, Morita, Tomoyo, additional, Kikuiri, Mizuha, additional, Amano, Kana, additional, Kagawa, Eiichi, additional, Noguchi, Takaaki, additional, Naraoka, Hiroshi, additional, Okazaki, Ryuji, additional, Sakamoto, Kanako, additional, Yurimoto, Hisayoshi, additional, Abe, Masanao, additional, Kamide, Kanami, additional, Miyazaki, Akiko, additional, Nakato, Aiko, additional, Nakazawa, Satoru, additional, Nishimura, Masahiro, additional, Okada, Tatsuaki, additional, Saiki, Takanao, additional, Tachibana, Shogo, additional, Tanaka, Satoshi, additional, Terui, Fuyuto, additional, Tsuda, Yuichi, additional, Usui, Tomohiro, additional, Watanabe, Sei-ichiro, additional, Yada, Toru, additional, Yogata, Kasumi, additional, and Yoshikawa, Makoto, additional

Published
2023
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26. Macromolecular organic matter in samples of the asteroid (162173) Ryugu

Author

Yabuta, Hikaru, primary, Cody, George D., additional, Engrand, Cécile, additional, Kebukawa, Yoko, additional, De Gregorio, Bradley, additional, Bonal, Lydie, additional, Remusat, Laurent, additional, Stroud, Rhonda, additional, Quirico, Eric, additional, Nittler, Larry, additional, Hashiguchi, Minako, additional, Komatsu, Mutsumi, additional, Okumura, Taiga, additional, Mathurin, Jérémie, additional, Dartois, Emmanuel, additional, Duprat, Jean, additional, Takahashi, Yoshio, additional, Takeichi, Yasuo, additional, Kilcoyne, David, additional, Yamashita, Shohei, additional, Dazzi, Alexandre, additional, Deniset-Besseau, Ariane, additional, Sandford, Scott, additional, Martins, Zita, additional, Tamenori, Yusuke, additional, Ohigashi, Takuji, additional, Suga, Hiroki, additional, Wakabayashi, Daisuke, additional, Verdier-Paoletti, Maximilien, additional, Mostefaoui, Smail, additional, Montagnac, Gilles, additional, Barosch, Jens, additional, Kamide, Kanami, additional, Shigenaka, Miho, additional, Bejach, Laure, additional, Matsumoto, Megumi, additional, Enokido, Yuma, additional, Noguchi, Takaaki, additional, Yurimoto, Hisayoshi, additional, Nakamura, Tomoki, additional, Okazaki, Ryuji, additional, Naraoka, Hiroshi, additional, Sakamoto, Kanako, additional, Connolly, Harold C., additional, Lauretta, Dante S., additional, Abe, Masanao, additional, Okada, Tatsuaki, additional, Yada, Toru, additional, Nishimura, Masahiro, additional, Yogata, Kasumi, additional, Nakato, Aiko, additional, Yoshitake, Miwa, additional, Iwamae, Ayako, additional, Furuya, Shizuho, additional, Hatakeda, Kentaro, additional, Miyazaki, Akiko, additional, Soejima, Hiromichi, additional, Hitomi, Yuya, additional, Kumagai, Kazuya, additional, Usui, Tomohiro, additional, Hayashi, Tasuku, additional, Yamamoto, Daiki, additional, Fukai, Ryota, additional, Sugita, Seiji, additional, Kitazato, Kohei, additional, Hirata, Naru, additional, Honda, Rie, additional, Morota, Tomokatsu, additional, Tatsumi, Eri, additional, Sakatani, Naoya, additional, Namiki, Noriyuki, additional, Matsumoto, Koji, additional, Noguchi, Rina, additional, Wada, Koji, additional, Senshu, Hiroki, additional, Ogawa, Kazunori, additional, Yokota, Yasuhiro, additional, Ishihara, Yoshiaki, additional, Shimaki, Yuri, additional, Yamada, Manabu, additional, Honda, Chikatoshi, additional, Michikami, Tatsuhiro, additional, Matsuoka, Moe, additional, Hirata, Naoyuki, additional, Arakawa, Masahiko, additional, Okamoto, Chisato, additional, Ishiguro, Masateru, additional, Jaumann, Ralf, additional, Bibring, Jean-Pierre, additional, Grott, Matthias, additional, Schröder, Stefan, additional, Otto, Katharina, additional, Pilorget, Cedric, additional, Schmitz, Nicole, additional, Biele, Jens, additional, Ho, Tra-Mi, additional, Moussi-Soffys, Aurélie, additional, Miura, Akira, additional, Noda, Hirotomo, additional, Yamada, Tetsuya, additional, Yoshihara, Keisuke, additional, Kawahara, Kosuke, additional, Ikeda, Hitoshi, additional, Yamamoto, Yukio, additional, Shirai, Kei, additional, Kikuchi, Shota, additional, Ogawa, Naoko, additional, Takeuchi, Hiroshi, additional, Ono, Go, additional, Mimasu, Yuya, additional, Yoshikawa, Kent, additional, Takei, Yuto, additional, Fujii, Atsushi, additional, Iijima, Yu-ichi, additional, Nakazawa, Satoru, additional, Hosoda, Satoshi, additional, Iwata, Takahiro, additional, Hayakawa, Masahiko, additional, Sawada, Hirotaka, additional, Yano, Hajime, additional, Tsukizaki, Ryudo, additional, Ozaki, Masanobu, additional, Terui, Fuyuto, additional, Tanaka, Satoshi, additional, Fujimoto, Masaki, additional, Yoshikawa, Makoto, additional, Saiki, Takanao, additional, Tachibana, Shogo, additional, Watanabe, Sei-ichiro, additional, and Tsuda, Yuichi, additional

Published
2023
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29. Cystinuria and cystinosis are usually related to L-cystine: is this really the case for cystinosis? A physicochemical investigation at micrometre and nanometre scale

Author

Bazin, Dominique, primary, Rabant, Marion, additional, Mathurin, Jérémie, additional, Petay, Margaux, additional, Deniset-Besseau, Ariane, additional, Dazzi, Alexandre, additional, Su, Yangyang, additional, Hessou, Etienne P., additional, Tielens, Frederik, additional, Borondics, Ferenc, additional, Livrozet, Marine, additional, Bouderlique, Elise, additional, Haymann, Jean-Philippe, additional, Letavernier, Emmanuel, additional, Frochot, Vincent, additional, and Daudon, Michel, additional

Published
2022
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30. Presolar Stardust in Asteroid Ryugu

Author

Barosch, Jens, primary, Nittler, Larry R., additional, Wang, Jianhua, additional, O’D. Alexander, Conel M., additional, De Gregorio, Bradley T., additional, Engrand, Cécile, additional, Kebukawa, Yoko, additional, Nagashima, Kazuhide, additional, Stroud, Rhonda M., additional, Yabuta, Hikaru, additional, Abe, Yoshinari, additional, Aléon, Jérôme, additional, Amari, Sachiko, additional, Amelin, Yuri, additional, Bajo, Ken-ichi, additional, Bejach, Laure, additional, Bizzarro, Martin, additional, Bonal, Lydie, additional, Bouvier, Audrey, additional, Carlson, Richard W., additional, Chaussidon, Marc, additional, Choi, Byeon-Gak, additional, Cody, George D., additional, Dartois, Emmanuel, additional, Dauphas, Nicolas, additional, Davis, Andrew M., additional, Dazzi, Alexandre, additional, Deniset-Besseau, Ariane, additional, Di Rocco, Tommaso, additional, Duprat, Jean, additional, Fujiya, Wataru, additional, Fukai, Ryota, additional, Gautam, Ikshu, additional, Haba, Makiko K., additional, Hashiguchi, Minako, additional, Hibiya, Yuki, additional, Hidaka, Hiroshi, additional, Homma, Hisashi, additional, Hoppe, Peter, additional, Huss, Gary R., additional, Ichida, Kiyohiro, additional, Iizuka, Tsuyoshi, additional, Ireland, Trevor R., additional, Ishikawa, Akira, additional, Ito, Motoo, additional, Itoh, Shoichi, additional, Kamide, Kanami, additional, Kawasaki, Noriyuki, additional, David Kilcoyne, A. L., additional, Kita, Noriko T., additional, Kitajima, Kouki, additional, Kleine, Thorsten, additional, Komatani, Shintaro, additional, Komatsu, Mutsumi, additional, Krot, Alexander N., additional, Liu, Ming-Chang, additional, Martins, Zita, additional, Masuda, Yuki, additional, Mathurin, Jérémie, additional, McKeegan, Kevin D., additional, Montagnac, Gilles, additional, Morita, Mayu, additional, Mostefaoui, Smail, additional, Motomura, Kazuko, additional, Moynier, Frédéric, additional, Nakai, Izumi, additional, Nguyen, Ann N., additional, Ohigashi, Takuji, additional, Okumura, Taiga, additional, Onose, Morihiko, additional, Pack, Andreas, additional, Park, Changkun, additional, Piani, Laurette, additional, Qin, Liping, additional, Quirico, Eric, additional, Remusat, Laurent, additional, Russell, Sara S., additional, Sakamoto, Naoya, additional, Sandford, Scott A., additional, Schönbächler, Maria, additional, Shigenaka, Miho, additional, Suga, Hiroki, additional, Tafla, Lauren, additional, Takahashi, Yoshio, additional, Takeichi, Yasuo, additional, Tamenori, Yusuke, additional, Tang, Haolan, additional, Terada, Kentaro, additional, Terada, Yasuko, additional, Usui, Tomohiro, additional, Verdier-Paoletti, Maximilien, additional, Wada, Sohei, additional, Wadhwa, Meenakshi, additional, Wakabayashi, Daisuke, additional, Walker, Richard J., additional, Yamashita, Katsuyuki, additional, Yamashita, Shohei, additional, Yin, Qing-Zhu, additional, Yokoyama, Tetsuya, additional, Yoneda, Shigekazu, additional, Young, Edward D., additional, Yui, Hiroharu, additional, Zhang, Ai-Cheng, additional, Abe, Masanao, additional, Miyazaki, Akiko, additional, Nakato, Aiko, additional, Nakazawa, Satoru, additional, Nishimura, Masahiro, additional, Okada, Tatsuaki, additional, Saiki, Takanao, additional, Tanaka, Satoshi, additional, Terui, Fuyuto, additional, Tsuda, Yuichi, additional, Watanabe, Sei-ichiro, additional, Yada, Toru, additional, Yogata, Kasumi, additional, Yoshikawa, Makoto, additional, Nakamura, Tomoki, additional, Naraoka, Hiroshi, additional, Noguchi, Takaaki, additional, Okazaki, Ryuji, additional, Sakamoto, Kanako, additional, Tachibana, Shogo, additional, and Yurimoto, Hisayoshi, additional

Published
2022
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32. A versatile and straightforward process to turn plastics into antibacterial materials

Author

Hadiouch, Slim, Maresca, Marc, Gigmes, Didier, Machado, Guilherme, Maurel-Pantel, Aurelien, Frik, Sabrina, Saunier, Johanna, Deniset-Besseau, Ariane, Yagoubi, Najet, Michalek, Lukas, Barner-Kowollik, Christopher, Guillaneuf, Yohann, Lefay, Catherine, Hadiouch, Slim, Maresca, Marc, Gigmes, Didier, Machado, Guilherme, Maurel-Pantel, Aurelien, Frik, Sabrina, Saunier, Johanna, Deniset-Besseau, Ariane, Yagoubi, Najet, Michalek, Lukas, Barner-Kowollik, Christopher, Guillaneuf, Yohann, and Lefay, Catherine

Abstract

We demonstrate that antibacterial activity can be conferred to common plastic materials using amphiphilic cationic methacrylate-based block copolymers, specifically quaternized poly(butyl methacrylate)-b-poly(N,N-dimethyl-aminoethyl methacrylate) (PBMA-b-PDMAEMA) with 64 mol% of DMAEMA and Mn close to 20 000 g mol-1. With 0.5-2 wt% of these copolymers simply dispersed in the given matrix by extrusion, the modified materials prove to be active against E. coli, S. aureus, Listeria monocytogenes and enterohemorrhagic E. coli without toxicity against two cell lines, HaCaT and L929 fibroblasts, while keeping the mechanical properties of the materials intact. In addition, the study of the mechanism of action shows that the antibacterial materials target the bacterial membrane, which is expected to avoid antibacterial resistance. Our protocol is a cost-effective solution to generate antibacterial materials for several applications, including packaging or medical devices, without modification of the production process. This journal is

Published
2022

36. A versatile and straightforward process to turn plastics into antibacterial materials

Author

Hadiouch, Slim, primary, Maresca, Marc, additional, Gigmes, Didier, additional, Machado, Guilherme, additional, Maurel-Pantel, Aurelien, additional, Frik, Sabrina, additional, Saunier, Johanna, additional, Deniset-Besseau, Ariane, additional, Yagoubi, Najet, additional, Michalek, Lukas, additional, Barner-Kowollik, Christopher, additional, Guillaneuf, Yohann, additional, and Lefay, Catherine, additional

Published
2022
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37. Revealing Lipid Body Formation and its Subcellular Reorganization in Oleaginous Microalgae Using Correlative Optical Microscopy and Infrared Nanospectroscopy Atomic Force Microscopy-Induced Resonance (AFM-IR)

Author

Deniset-Besseau, Ariane, Coat, Rémy, Moutel, Benjamin, Rebois, Rolando, Mathurin, Jérémie, Grizeau, Dominique, Dazzi, Alexandre, Gonçalves, Olivier, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Bioprocédés Appliqués aux Microalgues (GEPEA-BAM), Laboratoire de génie des procédés - environnement - agroalimentaire (GEPEA), Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN), Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Universite Paris-Saclay, 91198 ´ Gif-sur-Yvette, France, Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut Universitaire de Technologie - Nantes (IUT Nantes), Université de Nantes (UN)-Institut Universitaire de Technologie Saint-Nazaire (IUT Saint-Nazaire), Université de Nantes (UN)-Institut Universitaire de Technologie - La Roche-sur-Yon (IUT La Roche-sur-Yon), Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), and Université de Nantes (UN)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Université Bretagne Loire (UBL)

Subjects
[SPI]Engineering Sciences [physics], [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Abstract

International audience; The purpose of this work is to develop an integrated imaging approach to characterize without labeling at the sub-cellular level the formation of lipid body droplets (LBs) in microalgae undergoing nitrogen starvation. First conventional optical microscopy approaches, gas chromatography, and turbidimetry measurements allowed to monitor the biomass and the total lipid content in the oleaginous microalgae Parachlorella kesslerii during the starvation process. Then a local analysis of the LBs was proposed using an innovative infrared nanospectroscopy technique called atomic force microscopy-based infrared spectroscopy (AFM-IR). This label-free technique assessed the formation of LBs and allowed to look into the LB composition thanks to the acquisition of local infrared spectra. Last correlative measurements using fluorescence microscopy and AFM-IR were performed to investigate the subcellular reorganization of LB and the chloroplasts.

Published
2021
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43. sj-pdf-1-asp-10.1177_00037028211050659 - Supplemental material for Revealing Lipid Body Formation and its Subcellular Reorganization in Oleaginous Microalgae Using Correlative Optical Microscopy and Infrared Nanospectroscopy Atomic Force Microscopy-Induced Resonance (AFM-IR)

Author

Deniset-Besseau, Ariane, Coat, R��my, Moutel, Benjamin, Rebois, Rolando, Mathurin, J��r��mie, Grizeau, Dominique, Dazzi, Alexandre, and Gon��alves, Olivier

Subjects
FOS: Other engineering and technologies, 99999 Engineering not elsewhere classified
Abstract

Supplemental material, sj-pdf-1-asp-10.1177_00037028211050659 for Revealing Lipid Body Formation and its Subcellular Reorganization in Oleaginous Microalgae Using Correlative Optical Microscopy and Infrared Nanospectroscopy Atomic Force Microscopy-Induced Resonance (AFM-IR) by Ariane Deniset-Besseau, R��my Coat, Benjamin Moutel, Rolando Rebois, J��r��mie Mathurin, Dominique Grizeau, Alexandre Dazzi and Olivier Gon��alves in Applied Spectroscopy

Published
2021
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44. Probing amyloid fibril secondary structures by infrared nanospectroscopy: Experimental and theoretical considerations

Author

Waeytens, Jehan, Mathurin, Jérémie, Deniset-Besseau, Ariane, Arluison, Véronique, Bousset, Luc, Rezaei, Human, Raussens, Vincent, Dazzi, Alexandre, Waeytens, Jehan, Mathurin, Jérémie, Deniset-Besseau, Ariane, Arluison, Véronique, Bousset, Luc, Rezaei, Human, Raussens, Vincent, and Dazzi, Alexandre

Abstract

Amyloid fibrils are composed of aggregated peptides or proteins in a fibrillary structure with a higher β-sheet content than their native structure. Attenuated total reflection Fourier transform infrared spectroscopy only provides bulk analysis of a sample therefore it is impossible to discriminate between different aggregated structures. To overcome this limitation, near-field techniques like AFM-IR have emerged in the last twenty years to allow infrared nanospectroscopy. This technique obtains IR spectra with a spatial resolution of ten nanometres, the size of isolated fibrils. Here, we present essential practical considerations to avoid misinterpretations and artefacts during these analyses. Effects of polarization of the incident IR laser, illumination configuration and coating of the AFM probes are discussed, including the advantages and drawbacks of their use. This approach will improve interpretation of AFM-IR spectra especially for the determination of secondary structures of species not accessible using classical ATR-FTIR., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2021

45. Nanostructural Evolution of Natural Rubber/Silica Nanoparticle Coagulation from Binary Colloidal Suspensions to Composites: Implications for Tire Materials

Author

Cattinari, Gianluca, primary, Steenkeste, Karine, additional, Lévêque-Fort, Sandrine, additional, Cabriel, Clément, additional, Deniset-Besseau, Ariane, additional, Canette, Alexis, additional, Oberdisse, Julian, additional, Gallopin, Matthieu, additional, Couty, Marc, additional, and Fontaine-Aupart, Marie-Pierre, additional

Published
2021
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48. Characterization by Nano-Infrared Spectroscopy of Individual Aggregated Species of Amyloid Proteins.

Author

Waeytens, Jehan, Van Hemelryck, Vincent, Deniset-Besseau, Ariane, Ruysschaert, Jean Marie, Dazzi, Alexandre, Raussens, Vincent, Waeytens, Jehan, Van Hemelryck, Vincent, Deniset-Besseau, Ariane, Ruysschaert, Jean Marie, Dazzi, Alexandre, and Raussens, Vincent

Abstract

Amyloid fibrils are composed of aggregated peptides or proteins in a fibrillar structure with a higher β-sheet content than in their native structure. To characterize them, we used an innovative tool that coupled infrared spectroscopy with atomic force microscopy (AFM-IR). With this method, we show that we can detect different individual aggregated species from oligomers to fibrils and study their morphologies by AFM and their secondary structures based on their IR spectra. AFM-IR overcomes the weak spatial resolution of usual infrared spectroscopy and achieves a resolution of ten nanometers, the size of isolated fibrils. We characterized oligomers, amyloid fibrils of Aβ42 and fibrils of α-synuclein. To our surprise, we figured out that the nature of some surfaces (ZnSe) used to study the samples induces destructuring of amyloid samples, leading to amorphous aggregates. We strongly suggest taking this into consideration in future experiments with amyloid fibrils. More importantly, we demonstrate the advantages of AFM-IR, with a high spatial resolution (≤ 10 nm) allowing spectrum recording on individual aggregated supramolecular entities selected thanks to the AFM images or on thin layers of proteins., info:eu-repo/semantics/published

Published
2020

49. Nanometric chemical speciation of abnormal deposits in kidney biopsy: Infrared-nanospectroscopy reveals heterogeneities within vancomycin casts.

Author

Esteve, Emmanuel, Luque, Y, Waeytens, Jehan, Bazin, Dominique, Mesnard, L, Jouanneau, Chantal, Ronco, Pierre, Dazzi, Alexandre, Daudon, Michel, Deniset-Besseau, Ariane, Esteve, Emmanuel, Luque, Y, Waeytens, Jehan, Bazin, Dominique, Mesnard, L, Jouanneau, Chantal, Ronco, Pierre, Dazzi, Alexandre, Daudon, Michel, and Deniset-Besseau, Ariane

Abstract

info:eu-repo/semantics/published

Published
2020

50. The Phosin PptA Plays a Negative Role in the Regulation of Antibiotic Production in Streptomyces lividans

Author

Shikura, Noriyasu, primary, Darbon, Emmanuelle, additional, Esnault, Catherine, additional, Deniset-Besseau, Ariane, additional, Xu, Delin, additional, Lejeune, Clara, additional, Jacquet, Eric, additional, Nhiri, Naima, additional, Sago, Laila, additional, Cornu, David, additional, Werten, Sebastiaan, additional, Martel, Cécile, additional, and Virolle, Marie-Joelle, additional

Published
2021
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