1. Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation
- Author
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Denise Gusmão de Oliveira, Vinícius Carvalho Porto, Ana Paula Chappuis Chocano, Jefferson Ricardo Pereira, Flora Freitas Fernandes Távora, Karin Hermana Neppelenbroek, and Ricardo Almeida
- Subjects
Denture Bases ,Surface Properties ,PLACA BACTERIANA ,Acrylic Resins ,engineering.material ,confocal microscopy ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Coating ,Candida albicans ,Cyanoacrylates ,General Dentistry ,Acrylic resin ,biology ,Chemistry ,Sealant ,Biofilm ,030206 dentistry ,Sterilization (microbiology) ,biology.organism_classification ,Corpus albicans ,Ethyl cyanoacrylate ,Biofilms ,cyanoacrylates ,030220 oncology & carcinogenesis ,visual_art ,denture liners ,biofilm formation ,engineering ,visual_art.visual_art_medium ,candida albicans ,Brazil ,Nuclear chemistry - Abstract
The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p
- Published
- 2019